>RPS6|NM_001010|3'UTR
   1 GATTTTTTGAGTAACAAATAAATAAGATCAGACTCTG

ID	Duplex structure	Position	Score	MFE
1	miRNA 3' ucuccguccaaggacCUAGUa 5' ||||| Target 5' gtaacaaataaataaGATCAg 3'	11 - 31	100.00	-5.60

Mutant ID	Mutant Position	Mutant Source
COSN26993339	1	COSMIC
COSN30152106	1	COSMIC
COSN8892665	9	COSMIC
COSN31511629	26	COSMIC
COSN14386693	29	COSMIC
COSN31591789	31	COSMIC
COSN30157218	35	COSMIC
COSN22734571	42	COSMIC
COSN28191719	124	COSMIC
COSN2314926	334	COSMIC
rs67906804	33	GWAS
rs67710536	36	GWAS

Mutant ID	Mutant Position	Mutant Source
rs1012019872	1	dbSNP
rs1439605729	2	dbSNP
rs553421652	3	dbSNP
rs777831669	4	dbSNP
rs760802711	11	dbSNP
rs773586781	12	dbSNP
rs772673878	14	dbSNP
rs562946854	15	dbSNP
rs768929394	16	dbSNP
rs1442525977	17	dbSNP
rs1291097398	18	dbSNP
rs745442351	22	dbSNP
rs757445661	26	dbSNP
rs758470032	26	dbSNP
rs933881985	27	dbSNP
rs369681273	29	dbSNP
rs765126928	29	dbSNP
rs67906804	33	dbSNP
rs759052038	33	dbSNP
rs1055228895	34	dbSNP
rs781008957	35	dbSNP
rs67710536	36	dbSNP
rs1234635351	37	dbSNP
rs746587364	37	dbSNP
rs1279298293	38	dbSNP
rs1375985279	39	dbSNP
rs1351922977	45	dbSNP
rs758411939	46	dbSNP
rs1417604031	47	dbSNP
rs1429241953	48	dbSNP
rs753550645	49	dbSNP
rs994439195	51	dbSNP
rs367650988	52	dbSNP
rs754802484	56	dbSNP
rs1208072462	70	dbSNP
rs1269573310	73	dbSNP
rs1452058270	74	dbSNP
rs897066932	75	dbSNP
rs937704805	77	dbSNP
rs1038272670	81	dbSNP
rs1441291397	83	dbSNP
rs1159089489	85	dbSNP
rs1255495801	85	dbSNP
rs1384216855	86	dbSNP
rs927621630	87	dbSNP
rs1294726789	89	dbSNP
rs1390868355	92	dbSNP
rs1390357309	97	dbSNP
rs1328745030	105	dbSNP
rs1340687170	106	dbSNP
rs1187439551	109	dbSNP
rs1242765949	114	dbSNP
rs1358920419	115	dbSNP
rs562166683	115	dbSNP
rs1242908398	117	dbSNP
rs941353754	118	dbSNP
rs889798806	122	dbSNP
rs1049779861	124	dbSNP
rs115304926	126	dbSNP
rs1202824643	127	dbSNP
rs113373506	135	dbSNP
rs10119465	147	dbSNP
rs934865885	151	dbSNP
rs1216837257	154	dbSNP
rs926208062	156	dbSNP
rs979020644	157	dbSNP
rs1398660865	158	dbSNP
rs967681458	160	dbSNP
rs1332402807	168	dbSNP
rs775195856	178	dbSNP
rs1394691346	180	dbSNP
rs1315075995	183	dbSNP
rs1292163893	184	dbSNP
rs1017707206	185	dbSNP
rs1256240157	189	dbSNP
rs1356942707	192	dbSNP
rs1199230587	193	dbSNP
rs1296162029	196	dbSNP
rs540173060	197	dbSNP
rs371528908	202	dbSNP
rs1172360367	203	dbSNP
rs1035218830	205	dbSNP
rs1256421748	206	dbSNP
rs1415704671	206	dbSNP
rs551722797	207	dbSNP
rs1190807194	214	dbSNP
rs1425173569	233	dbSNP
rs1467604176	233	dbSNP
rs1171966994	239	dbSNP
rs879137949	243	dbSNP
rs368590261	245	dbSNP
rs1359142444	249	dbSNP
rs1475597670	250	dbSNP
rs897007007	252	dbSNP
rs1464846593	255	dbSNP
rs1030336018	264	dbSNP
rs1380074304	265	dbSNP
rs1399642597	268	dbSNP
rs1276341842	272	dbSNP
rs1016780099	273	dbSNP
rs1342420346	273	dbSNP
rs181741745	274	dbSNP
rs1282546685	275	dbSNP
rs557594963	281	dbSNP
rs1211899946	282	dbSNP
rs897083085	284	dbSNP
rs1485314572	288	dbSNP
rs188634227	291	dbSNP
rs1264929962	292	dbSNP
rs1477847125	295	dbSNP
rs1198132240	296	dbSNP
rs1425074872	296	dbSNP
rs764338890	298	dbSNP
rs1233852284	305	dbSNP
rs1178610260	307	dbSNP
rs914836359	308	dbSNP
rs1207185187	318	dbSNP
rs888261335	318	dbSNP
rs1296559464	320	dbSNP
rs1467513018	325	dbSNP
rs1273156931	326	dbSNP
rs1054912828	328	dbSNP
rs1362755831	328	dbSNP
rs1293024488	329	dbSNP
rs566896294	333	dbSNP
rs1220899449	339	dbSNP
rs3198076	342	dbSNP
rs1352670780	346	dbSNP
rs1237747270	348	dbSNP
rs183996616	348	dbSNP
rs1336385933	353	dbSNP
rs927670712	356	dbSNP
rs981743864	357	dbSNP
rs1244490212	359	dbSNP
rs1373737077	360	dbSNP
rs1487762693	361	dbSNP
rs1299687129	362	dbSNP
rs986828411	368	dbSNP
rs1049727917	370	dbSNP
rs958254007	375	dbSNP
rs1382334052	376	dbSNP
rs138886167	379	dbSNP
rs901255266	382	dbSNP
rs1053637990	383	dbSNP
rs1462015386	384	dbSNP
rs1438748527	385	dbSNP
rs1294014481	387	dbSNP
rs934833989	388	dbSNP
rs1311190175	389	dbSNP
rs1157146060	390	dbSNP
rs1353902107	390	dbSNP
rs1227405687	391	dbSNP
rs1287079283	392	dbSNP
rs1418746860	392	dbSNP
rs954780604	393	dbSNP
rs1221740072	398	dbSNP
rs1254170395	399	dbSNP
rs1457716412	403	dbSNP
rs1177102070	405	dbSNP
rs1240443583	406	dbSNP
rs540996275	410	dbSNP
rs764824143	411	dbSNP
rs1030388263	413	dbSNP
rs1409057516	413	dbSNP
rs993510830	413	dbSNP
rs1165649596	416	dbSNP
rs1399368405	419	dbSNP
rs1464011127	425	dbSNP
rs1043290018	426	dbSNP
rs1333059701	426	dbSNP
rs1447080777	433	dbSNP
rs1180870345	435	dbSNP
rs1282054847	436	dbSNP
rs1380973738	437	dbSNP
rs948957695	441	dbSNP
rs866888528	442	dbSNP
rs566684166	443	dbSNP
rs41270109	444	dbSNP
rs1252906425	447	dbSNP
rs990480599	450	dbSNP
rs1054880829	451	dbSNP
rs1438727287	453	dbSNP
rs71496816	453	dbSNP
rs796408109	453	dbSNP
rs953648286	460	dbSNP
rs1214879338	461	dbSNP
rs1235937493	463	dbSNP
rs1046100822	465	dbSNP
rs1182462007	471	dbSNP
rs1423035745	473	dbSNP
rs1463962185	475	dbSNP
rs1172691306	476	dbSNP
rs1469365482	482	dbSNP
rs1258467397	485	dbSNP
rs1401016774	487	dbSNP
rs1413697687	489	dbSNP
rs960674071	490	dbSNP
rs765854116	492	dbSNP
rs1317115817	496	dbSNP
rs927720701	498	dbSNP
rs1231795821	499	dbSNP
rs1276979924	503	dbSNP
rs1337733490	504	dbSNP
rs1370048551	504	dbSNP
rs972909235	505	dbSNP
rs1054070475	507	dbSNP
rs533298373	511	dbSNP
rs1340764672	512	dbSNP
rs1392913653	521	dbSNP
rs1276963125	523	dbSNP
rs1287171029	528	dbSNP
rs569066805	530	dbSNP
rs1211904718	532	dbSNP
rs761400368	533	dbSNP
rs1453201116	535	dbSNP
rs986306897	536	dbSNP
rs143324464	540	dbSNP
rs923368321	542	dbSNP
rs1477665661	543	dbSNP
rs953957290	543	dbSNP
rs1427048755	548	dbSNP
rs371789176	548	dbSNP
rs1297181075	549	dbSNP
rs1016672226	554	dbSNP
rs548423959	554	dbSNP
rs998179747	555	dbSNP
rs1298278209	557	dbSNP
rs1433185131	558	dbSNP
rs1273399884	559	dbSNP
rs1394863213	559	dbSNP
rs1235536708	561	dbSNP
rs1347537695	561	dbSNP
rs1285575628	565	dbSNP
rs562048924	566	dbSNP
rs1435444381	568	dbSNP
rs1218812121	577	dbSNP
rs1005508191	579	dbSNP

- Genes & development, 2013

When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.