pre-miRNA Information | |
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pre-miRNA | hsa-mir-6132 |
Genomic Coordinates | chr7: 117020211 - 117020319 |
Description | Homo sapiens miR-6132 stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Mature miRNA Information | ||||||||||||||||
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Mature miRNA | hsa-miR-6132 | |||||||||||||||
Sequence | 21| AGCAGGGCUGGGGAUUGCA |39 | |||||||||||||||
Evidence | Experimental | |||||||||||||||
Experiments | Illumina | |||||||||||||||
SNPs in miRNA |
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Putative Targets |
miRNA Expression profile | |
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Human miRNA Tissue Atlas | |
Circulating MicroRNA Expression Profiling |
Gene Information | |||||||||||||||||||||
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Gene Symbol | VDAC2 | ||||||||||||||||||||
Synonyms | POR | ||||||||||||||||||||
Description | voltage dependent anion channel 2 | ||||||||||||||||||||
Transcript | NM_001184823 | ||||||||||||||||||||
Other Transcripts | NM_001184783 , NM_003375 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on VDAC2 | |||||||||||||||||||||
3'UTR of VDAC2 (miRNA target sites are highlighted) |
>VDAC2|NM_001184823|3'UTR 1 TCCAGCTGAAAGAAACCTTTGGGAATGGATATCAGAAGATTTGGCCTTAATATATTTCCATTGTGACCAGCAGCAGGCTT 81 TTTTCCCCCAAGAAGATGATCAAAACAAAGGATGATCTCAACAAGAGCTGTATTTTAAGTATTTAGACAGTTCTTTGTTA 161 GCTGGTTTCTAGTTGGTTATCTAGTTACCAATGCTGCAGTCCTGCAGTCACCTATACATTATTTAAATGTATTTAACTGT 241 TAAATGCGCTACCCACCAATAATGAAATAGACCTTTATGAAAACTGTGCAATTGTGTGCATGTTTGTTTTTATGTTCCTT 321 TAGAAAACATTGACTGTTACCATTGAATGAGATGGATCAGTGGATATTAAGATGAGGTTACAAATTTTGTTAAGTTCAGC 401 CATTATTACTTTTGGTATCCCAGAACATGACAAATTATGAATAAAACAAGTATACATAAAAAAAAAAAAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | HEK293S |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM1084066. RNA binding protein: AGO2. Condition:CLIP_noemetine_SantaCruzAb
HITS-CLIP data was present in GSM1084080. RNA binding protein: AGO2. Condition:CLIP_nohippuristanol_rep2_SantaCruzAb
HITS-CLIP data was present in GSM1084081. RNA binding protein: AGO2. Condition:CLIP_hippuristanol_rep2_SantaCruzAb
... - Karginov FV; Hannon GJ, 2013, Genes & development. |
Article |
- Karginov FV; Hannon GJ - Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
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CLIP-seq Support 1 for dataset GSM1084066 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_noemetine_SantaCruzAb |
Location of target site | ENST00000394893.1 | 3UTR | GGCAGGGAGGACAAGGC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset GSM1084081 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_hippuristanol_rep2_SantaCruzAb |
Location of target site | ENST00000394893.1 | 3UTR | GGCAGGGAGGACAAGGC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
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MiRNA-Target Expression Profile (TCGA) | |||||||
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82 hsa-miR-6132 Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT067294 | NECAP1 | NECAP endocytosis associated 1 | ![]() |
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2 | 10 | ||||||
MIRT100110 | ABT1 | activator of basal transcription 1 | ![]() |
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2 | 8 | ||||||
MIRT358583 | CANX | calnexin | ![]() |
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2 | 2 | ||||||
MIRT445247 | SEMA5A | semaphorin 5A | ![]() |
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2 | 2 | ||||||
MIRT445764 | CCND3 | cyclin D3 | ![]() |
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2 | 2 | ||||||
MIRT452388 | LY6E | lymphocyte antigen 6 family member E | ![]() |
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2 | 4 | ||||||
MIRT452829 | FAM131B | family with sequence similarity 131 member B | ![]() |
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2 | 2 | ||||||
MIRT453450 | GLG1 | golgi glycoprotein 1 | ![]() |
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2 | 2 | ||||||
MIRT455435 | ID3 | inhibitor of DNA binding 3, HLH protein | ![]() |
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2 | 2 | ||||||
MIRT460629 | IGFBP4 | insulin like growth factor binding protein 4 | ![]() |
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2 | 2 | ||||||
MIRT461607 | DPH2 | DPH2 homolog | ![]() |
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2 | 2 | ||||||
MIRT461989 | PACSIN1 | protein kinase C and casein kinase substrate in neurons 1 | ![]() |
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2 | 2 | ||||||
MIRT464258 | VCL | vinculin | ![]() |
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2 | 2 | ||||||
MIRT465713 | TNFAIP1 | TNF alpha induced protein 1 | ![]() |
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2 | 2 | ||||||
MIRT466475 | TECPR2 | tectonin beta-propeller repeat containing 2 | ![]() |
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2 | 7 | ||||||
MIRT467119 | SRGAP1 | SLIT-ROBO Rho GTPase activating protein 1 | ![]() |
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2 | 4 | ||||||
MIRT468299 | SFT2D2 | SFT2 domain containing 2 | ![]() |
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2 | 2 | ||||||
MIRT469696 | RAB5B | RAB5B, member RAS oncogene family | ![]() |
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2 | 8 | ||||||
MIRT469904 | PTRF | caveolae associated protein 1 | ![]() |
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2 | 2 | ||||||
MIRT470015 | PTPLB | 3-hydroxyacyl-CoA dehydratase 2 | ![]() |
1 | 1 | |||||||
MIRT471385 | PDPR | pyruvate dehydrogenase phosphatase regulatory subunit | ![]() |
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2 | 2 | ||||||
MIRT471409 | PDP2 | pyruvate dehyrogenase phosphatase catalytic subunit 2 | ![]() |
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2 | 2 | ||||||
MIRT471719 | OTUB1 | OTU deubiquitinase, ubiquitin aldehyde binding 1 | ![]() |
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2 | 2 | ||||||
MIRT473608 | MARK2 | microtubule affinity regulating kinase 2 | ![]() |
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2 | 2 | ||||||
MIRT476328 | GLTSCR1L | BRD4 interacting chromatin remodeling complex associated protein like | ![]() |
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2 | 2 | ||||||
MIRT479448 | CDK6 | cyclin dependent kinase 6 | ![]() |
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2 | 2 | ||||||
MIRT482032 | AMER1 | APC membrane recruitment protein 1 | ![]() |
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2 | 2 | ||||||
MIRT482360 | AGO2 | argonaute 2, RISC catalytic component | ![]() |
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2 | 2 | ||||||
MIRT484661 | HOXD3 | homeobox D3 | ![]() |
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2 | 4 | ||||||
MIRT486828 | NDOR1 | NADPH dependent diflavin oxidoreductase 1 | ![]() |
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2 | 2 | ||||||
MIRT487069 | CLASP1 | cytoplasmic linker associated protein 1 | ![]() |
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2 | 4 | ||||||
MIRT487196 | NFASC | neurofascin | ![]() |
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2 | 4 | ||||||
MIRT487448 | TFAP2B | transcription factor AP-2 beta | ![]() |
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2 | 4 | ||||||
MIRT487517 | GXYLT2 | glucoside xylosyltransferase 2 | ![]() |
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2 | 2 | ||||||
MIRT487640 | BRSK2 | BR serine/threonine kinase 2 | ![]() |
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2 | 4 | ||||||
MIRT487755 | SKI | SKI proto-oncogene | ![]() |
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2 | 4 | ||||||
MIRT489944 | CPLX1 | complexin 1 | ![]() |
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2 | 2 | ||||||
MIRT491101 | MSI1 | musashi RNA binding protein 1 | ![]() |
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2 | 4 | ||||||
MIRT491181 | LAMA5 | laminin subunit alpha 5 | ![]() |
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2 | 2 | ||||||
MIRT492355 | SEMA7A | semaphorin 7A (John Milton Hagen blood group) | ![]() |
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2 | 2 | ||||||
MIRT493918 | FAM127B | retrotransposon Gag like 8A | ![]() |
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2 | 4 | ||||||
MIRT493932 | FAM127A | retrotransposon Gag like 8C | ![]() |
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2 | 4 | ||||||
MIRT494679 | ARID3A | AT-rich interaction domain 3A | ![]() |
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2 | 2 | ||||||
MIRT494814 | AKAP11 | A-kinase anchoring protein 11 | ![]() |
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2 | 2 | ||||||
MIRT494835 | ADCY9 | adenylate cyclase 9 | ![]() |
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2 | 2 | ||||||
MIRT495455 | PNMAL2 | paraneoplastic Ma antigen family member 8B | ![]() |
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2 | 2 | ||||||
MIRT496962 | MAP1LC3B | microtubule associated protein 1 light chain 3 beta | ![]() |
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2 | 2 | ||||||
MIRT526228 | MTRNR2L5 | MT-RNR2-like 5 | ![]() |
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2 | 2 | ||||||
MIRT531028 | TDGF1P3 | teratocarcinoma-derived growth factor 1 pseudogene 3 | ![]() |
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2 | 2 | ||||||
MIRT557110 | HOXA3 | homeobox A3 | ![]() |
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2 | 2 | ||||||
MIRT560514 | POGK | pogo transposable element derived with KRAB domain | ![]() |
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2 | 2 | ||||||
MIRT567753 | DLC1 | DLC1 Rho GTPase activating protein | ![]() |
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2 | 2 | ||||||
MIRT569608 | TRIM29 | tripartite motif containing 29 | ![]() |
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2 | 2 | ||||||
MIRT570242 | CPNE5 | copine 5 | ![]() |
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2 | 2 | ||||||
MIRT572327 | HSPB6 | heat shock protein family B (small) member 6 | ![]() |
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2 | 2 | ||||||
MIRT572373 | ATOX1 | antioxidant 1 copper chaperone | ![]() |
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2 | 2 | ||||||
MIRT575024 | Tecpr2 | tectonin beta-propeller repeat containing 2 | ![]() |
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2 | 5 | ||||||
MIRT576146 | Hmox1 | heme oxygenase 1 | ![]() |
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2 | 2 | ||||||
MIRT612443 | SMOC2 | SPARC related modular calcium binding 2 | ![]() |
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2 | 2 | ||||||
MIRT615404 | VDAC2 | voltage dependent anion channel 2 | ![]() |
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2 | 2 | ||||||
MIRT629114 | CYCS | cytochrome c, somatic | ![]() |
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2 | 2 | ||||||
MIRT631362 | FOXI2 | forkhead box I2 | ![]() |
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2 | 2 | ||||||
MIRT639322 | THBD | thrombomodulin | ![]() |
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2 | 2 | ||||||
MIRT643797 | ABCC12 | ATP binding cassette subfamily C member 12 | ![]() |
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2 | 2 | ||||||
MIRT669687 | ABLIM1 | actin binding LIM protein 1 | ![]() |
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2 | 2 | ||||||
MIRT670595 | LLGL1 | LLGL1, scribble cell polarity complex component | ![]() |
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2 | 4 | ||||||
MIRT691190 | NIF3L1 | NGG1 interacting factor 3 like 1 | ![]() |
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2 | 2 | ||||||
MIRT691688 | FLOT2 | flotillin 2 | ![]() |
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2 | 2 | ||||||
MIRT697127 | OTUD5 | OTU deubiquitinase 5 | ![]() |
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2 | 2 | ||||||
MIRT700814 | PHLDA2 | pleckstrin homology like domain family A member 2 | ![]() |
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2 | 2 | ||||||
MIRT701248 | NUP35 | nucleoporin 35 | ![]() |
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2 | 2 | ||||||
MIRT702362 | KLHL15 | kelch like family member 15 | ![]() |
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2 | 2 | ||||||
MIRT703325 | GDPD5 | glycerophosphodiester phosphodiesterase domain containing 5 | ![]() |
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2 | 2 | ||||||
MIRT706060 | PKD1 | polycystin 1, transient receptor potential channel interacting | ![]() |
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2 | 2 | ||||||
MIRT710475 | CDH5 | cadherin 5 | ![]() |
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2 | 2 | ||||||
MIRT713018 | SLC4A2 | solute carrier family 4 member 2 | ![]() |
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2 | 2 | ||||||
MIRT716427 | RAB15 | RAB15, member RAS oncogene family | ![]() |
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2 | 2 | ||||||
MIRT718093 | ABHD12 | abhydrolase domain containing 12 | ![]() |
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2 | 2 | ||||||
MIRT718542 | PIGQ | phosphatidylinositol glycan anchor biosynthesis class Q | ![]() |
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2 | 2 | ||||||
MIRT719122 | CACFD1 | calcium channel flower domain containing 1 | ![]() |
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2 | 2 | ||||||
MIRT721393 | LDLRAD4 | low density lipoprotein receptor class A domain containing 4 | ![]() |
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2 | 2 | ||||||
MIRT736283 | CDC42 | cell division cycle 42 | ![]() |
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2 | 0 |
miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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