pre-miRNA Information
pre-miRNA hsa-mir-6132   
Genomic Coordinates chr7: 117020211 - 117020319
Description Homo sapiens miR-6132 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-6132
Sequence 21| AGCAGGGCUGGGGAUUGCA |39
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1272646274 4 dbSNP
rs1211969145 8 dbSNP
rs1310278496 11 dbSNP
rs1044115534 13 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol VDAC2   
Synonyms POR
Description voltage dependent anion channel 2
Transcript NM_001184823   
Other Transcripts NM_001184783 , NM_003375   
Expression
Putative miRNA Targets on VDAC2
3'UTR of VDAC2
(miRNA target sites are highlighted)
>VDAC2|NM_001184823|3'UTR
   1 TCCAGCTGAAAGAAACCTTTGGGAATGGATATCAGAAGATTTGGCCTTAATATATTTCCATTGTGACCAGCAGCAGGCTT
  81 TTTTCCCCCAAGAAGATGATCAAAACAAAGGATGATCTCAACAAGAGCTGTATTTTAAGTATTTAGACAGTTCTTTGTTA
 161 GCTGGTTTCTAGTTGGTTATCTAGTTACCAATGCTGCAGTCCTGCAGTCACCTATACATTATTTAAATGTATTTAACTGT
 241 TAAATGCGCTACCCACCAATAATGAAATAGACCTTTATGAAAACTGTGCAATTGTGTGCATGTTTGTTTTTATGTTCCTT
 321 TAGAAAACATTGACTGTTACCATTGAATGAGATGGATCAGTGGATATTAAGATGAGGTTACAAATTTTGTTAAGTTCAGC
 401 CATTATTACTTTTGGTATCCCAGAACATGACAAATTATGAATAAAACAAGTATACATAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' acGUUA-GGGGUCGGGACGa 5'
            |||| |: |||:||||| 
Target 5' acCAATGCTGCAGTCCTGCa 3'
187 - 206 149.00 -19.40
2
miRNA  3' acgUUAGGGGUCGGGAcga 5'
             ||  ::::|||:|   
Target 5' cagAAGATTTGGCCTTaat 3'
33 - 51 92.00 -5.12
3
miRNA  3' acguUAGGGGU----CGGGACGa 5'
              ||::|||    | || || 
Target 5' atatATTTCCATTGTGACCAGCa 3'
50 - 72 89.00 -7.54
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN31532412 4 COSMIC
COSN30528470 27 COSMIC
COSN8863016 30 COSMIC
COSN30128546 72 COSMIC
COSN1122574 266 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs775155901 3 dbSNP
rs1407593475 5 dbSNP
rs760626166 21 dbSNP
rs1804199 30 dbSNP
rs753641029 33 dbSNP
rs989209619 34 dbSNP
rs761490870 41 dbSNP
rs1219655549 42 dbSNP
rs765122164 48 dbSNP
rs1159791786 49 dbSNP
rs1340079130 52 dbSNP
rs1438994520 60 dbSNP
rs1273285660 63 dbSNP
rs914965468 71 dbSNP
rs1213558091 79 dbSNP
rs1256249568 85 dbSNP
rs947572313 86 dbSNP
rs1336956896 89 dbSNP
rs11527434 91 dbSNP
rs553789955 95 dbSNP
rs1225941974 96 dbSNP
rs565733559 101 dbSNP
rs1303035177 102 dbSNP
rs539547562 104 dbSNP
rs763572441 106 dbSNP
rs1329869769 107 dbSNP
rs938939680 116 dbSNP
rs1450294007 121 dbSNP
rs1057365860 122 dbSNP
rs962285093 132 dbSNP
rs993690050 138 dbSNP
rs1178281808 142 dbSNP
rs1030481148 157 dbSNP
rs1428541676 159 dbSNP
rs954888231 170 dbSNP
rs368379528 174 dbSNP
rs1232950336 176 dbSNP
rs1243020215 179 dbSNP
rs1477305331 188 dbSNP
rs1804198 192 dbSNP
rs958322265 195 dbSNP
rs1324657804 197 dbSNP
rs929937609 211 dbSNP
rs1283223233 212 dbSNP
rs1375687425 218 dbSNP
rs7304 219 dbSNP
rs15249 231 dbSNP
rs1294668600 232 dbSNP
rs1441800927 238 dbSNP
rs888524954 247 dbSNP
rs543225247 248 dbSNP
rs1461494744 258 dbSNP
rs1162636397 261 dbSNP
rs1018102844 269 dbSNP
rs1388444395 276 dbSNP
rs1402332239 285 dbSNP
rs1408142938 287 dbSNP
rs111321825 290 dbSNP
rs1318751735 294 dbSNP
rs901118236 300 dbSNP
rs927815086 301 dbSNP
rs1399633019 313 dbSNP
rs1183764238 317 dbSNP
rs1333467285 322 dbSNP
rs1276538539 324 dbSNP
rs761599349 330 dbSNP
rs1245583707 331 dbSNP
rs1319216434 332 dbSNP
rs1055321411 335 dbSNP
rs1030854604 341 dbSNP
rs956389159 343 dbSNP
rs1259502 354 dbSNP
rs989095128 356 dbSNP
rs75096837 357 dbSNP
rs969163458 361 dbSNP
rs574018581 364 dbSNP
rs993742367 373 dbSNP
rs927493421 377 dbSNP
rs1158229255 378 dbSNP
rs541326156 382 dbSNP
rs890659387 384 dbSNP
rs1386493946 395 dbSNP
rs1183877781 398 dbSNP
rs1259147491 408 dbSNP
rs993097437 409 dbSNP
rs1180506172 413 dbSNP
rs1487025051 416 dbSNP
rs918812877 417 dbSNP
rs187746320 420 dbSNP
rs1186265772 421 dbSNP
rs532912333 422 dbSNP
rs1366152056 424 dbSNP
rs1351684339 435 dbSNP
rs1048404664 439 dbSNP
rs1471126236 441 dbSNP
rs909994122 453 dbSNP
rs1358960465 459 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084066. RNA binding protein: AGO2. Condition:CLIP_noemetine_SantaCruzAb HITS-CLIP data was present in GSM1084080. RNA binding protein: AGO2. Condition:CLIP_nohippuristanol_rep2_SantaCruzAb HITS-CLIP data was present in GSM1084081. RNA binding protein: AGO2. Condition:CLIP_hippuristanol_rep2_SantaCruzAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084066
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_noemetine_SantaCruzAb
Location of target site ENST00000394893.1 | 3UTR | GGCAGGGAGGACAAGGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM1084081
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_hippuristanol_rep2_SantaCruzAb
Location of target site ENST00000394893.1 | 3UTR | GGCAGGGAGGACAAGGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
82 hsa-miR-6132 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT067294 NECAP1 NECAP endocytosis associated 1 2 10
MIRT100110 ABT1 activator of basal transcription 1 2 8
MIRT358583 CANX calnexin 2 2
MIRT445247 SEMA5A semaphorin 5A 2 2
MIRT445764 CCND3 cyclin D3 2 2
MIRT452388 LY6E lymphocyte antigen 6 family member E 2 4
MIRT452829 FAM131B family with sequence similarity 131 member B 2 2
MIRT453450 GLG1 golgi glycoprotein 1 2 2
MIRT455435 ID3 inhibitor of DNA binding 3, HLH protein 2 2
MIRT460629 IGFBP4 insulin like growth factor binding protein 4 2 2
MIRT461607 DPH2 DPH2 homolog 2 2
MIRT461989 PACSIN1 protein kinase C and casein kinase substrate in neurons 1 2 2
MIRT464258 VCL vinculin 2 2
MIRT465713 TNFAIP1 TNF alpha induced protein 1 2 2
MIRT466475 TECPR2 tectonin beta-propeller repeat containing 2 2 7
MIRT467119 SRGAP1 SLIT-ROBO Rho GTPase activating protein 1 2 4
MIRT468299 SFT2D2 SFT2 domain containing 2 2 2
MIRT469696 RAB5B RAB5B, member RAS oncogene family 2 8
MIRT469904 PTRF caveolae associated protein 1 2 2
MIRT470015 PTPLB 3-hydroxyacyl-CoA dehydratase 2 1 1
MIRT471385 PDPR pyruvate dehydrogenase phosphatase regulatory subunit 2 2
MIRT471409 PDP2 pyruvate dehyrogenase phosphatase catalytic subunit 2 2 2
MIRT471719 OTUB1 OTU deubiquitinase, ubiquitin aldehyde binding 1 2 2
MIRT473608 MARK2 microtubule affinity regulating kinase 2 2 2
MIRT476328 GLTSCR1L BRD4 interacting chromatin remodeling complex associated protein like 2 2
MIRT479448 CDK6 cyclin dependent kinase 6 2 2
MIRT482032 AMER1 APC membrane recruitment protein 1 2 2
MIRT482360 AGO2 argonaute 2, RISC catalytic component 2 2
MIRT484661 HOXD3 homeobox D3 2 4
MIRT486828 NDOR1 NADPH dependent diflavin oxidoreductase 1 2 2
MIRT487069 CLASP1 cytoplasmic linker associated protein 1 2 4
MIRT487196 NFASC neurofascin 2 4
MIRT487448 TFAP2B transcription factor AP-2 beta 2 4
MIRT487517 GXYLT2 glucoside xylosyltransferase 2 2 2
MIRT487640 BRSK2 BR serine/threonine kinase 2 2 4
MIRT487755 SKI SKI proto-oncogene 2 4
MIRT489944 CPLX1 complexin 1 2 2
MIRT491101 MSI1 musashi RNA binding protein 1 2 4
MIRT491181 LAMA5 laminin subunit alpha 5 2 2
MIRT492355 SEMA7A semaphorin 7A (John Milton Hagen blood group) 2 2
MIRT493918 FAM127B retrotransposon Gag like 8A 2 4
MIRT493932 FAM127A retrotransposon Gag like 8C 2 4
MIRT494679 ARID3A AT-rich interaction domain 3A 2 2
MIRT494814 AKAP11 A-kinase anchoring protein 11 2 2
MIRT494835 ADCY9 adenylate cyclase 9 2 2
MIRT495455 PNMAL2 paraneoplastic Ma antigen family member 8B 2 2
MIRT496962 MAP1LC3B microtubule associated protein 1 light chain 3 beta 2 2
MIRT526228 MTRNR2L5 MT-RNR2-like 5 2 2
MIRT531028 TDGF1P3 teratocarcinoma-derived growth factor 1 pseudogene 3 2 2
MIRT557110 HOXA3 homeobox A3 2 2
MIRT560514 POGK pogo transposable element derived with KRAB domain 2 2
MIRT567753 DLC1 DLC1 Rho GTPase activating protein 2 2
MIRT569608 TRIM29 tripartite motif containing 29 2 2
MIRT570242 CPNE5 copine 5 2 2
MIRT572327 HSPB6 heat shock protein family B (small) member 6 2 2
MIRT572373 ATOX1 antioxidant 1 copper chaperone 2 2
MIRT575024 Tecpr2 tectonin beta-propeller repeat containing 2 2 5
MIRT576146 Hmox1 heme oxygenase 1 2 2
MIRT612443 SMOC2 SPARC related modular calcium binding 2 2 2
MIRT615404 VDAC2 voltage dependent anion channel 2 2 2
MIRT629114 CYCS cytochrome c, somatic 2 2
MIRT631362 FOXI2 forkhead box I2 2 2
MIRT639322 THBD thrombomodulin 2 2
MIRT643797 ABCC12 ATP binding cassette subfamily C member 12 2 2
MIRT669687 ABLIM1 actin binding LIM protein 1 2 2
MIRT670595 LLGL1 LLGL1, scribble cell polarity complex component 2 4
MIRT691190 NIF3L1 NGG1 interacting factor 3 like 1 2 2
MIRT691688 FLOT2 flotillin 2 2 2
MIRT697127 OTUD5 OTU deubiquitinase 5 2 2
MIRT700814 PHLDA2 pleckstrin homology like domain family A member 2 2 2
MIRT701248 NUP35 nucleoporin 35 2 2
MIRT702362 KLHL15 kelch like family member 15 2 2
MIRT703325 GDPD5 glycerophosphodiester phosphodiesterase domain containing 5 2 2
MIRT706060 PKD1 polycystin 1, transient receptor potential channel interacting 2 2
MIRT710475 CDH5 cadherin 5 2 2
MIRT713018 SLC4A2 solute carrier family 4 member 2 2 2
MIRT716427 RAB15 RAB15, member RAS oncogene family 2 2
MIRT718093 ABHD12 abhydrolase domain containing 12 2 2
MIRT718542 PIGQ phosphatidylinositol glycan anchor biosynthesis class Q 2 2
MIRT719122 CACFD1 calcium channel flower domain containing 1 2 2
MIRT721393 LDLRAD4 low density lipoprotein receptor class A domain containing 4 2 2
MIRT736283 CDC42 cell division cycle 42 2 0
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-6132 Vemurafenib 42611257 NSC761431 approved resistant High Melanoma cell line (A375, IGR37, 501Mel)
hsa-miR-6132 Dabrafenib 44462760 NSC764134 approved resistant High Melanoma cell line (A375, IGR37, 501Mel)
hsa-miR-6132 Paclitaxel 36314 NSC125973 approved resistant High Non-Small Cell Lung Cancer cell line (A549)
hsa-miR-6132 Cisplatin 5460033 NSC119875 approved resistant High Hypopharyngeal Cancer cell line (FaDu)
hsa-miR-6132 Temozolomide 5394 NSC362856 approved sensitive cell line (U251)
hsa-miR-6132 Cisplatin 5460033 NSC119875 approved sensitive cell line
hsa-miR-6132 Doxorubicin 31703 NSC123127 approved resistant cell line (HS578T)
hsa-miR-6132 Osimertinib 71496458 NSC779217 approved resistant cell line (H1975)
hsa-miR-6132 Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (100 ng/ml)

Error report submission