pre-miRNA Information
pre-miRNA hsa-mir-3133   
Genomic Coordinates chr2: 241477905 - 241477982
Description Homo sapiens miR-3133 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-3133
Sequence 10| UAAAGAACUCUUAAAACCCAAU |31
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1030052469 1 dbSNP
rs955010137 12 dbSNP
Putative Targets

miRNA Expression profile
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol ORAI1   
Synonyms CRACM1, IMD9, ORAT1, TAM2, TMEM142A
Description ORAI calcium release-activated calcium modulator 1
Transcript NM_032790   
Expression
Putative miRNA Targets on ORAI1
3'UTR of ORAI1
(miRNA target sites are highlighted)
>ORAI1|NM_032790|3'UTR
   1 GCCCATGTGGTCTGGGCCCTTCCAGTGCTTTGGCCTTACGCCCTTCCCCTTGACCTTGTCCTGCCCCAGCCTCACGGACA
  81 GCCTGCGCAGGGGGCTGGGCTTCAGCAAGGGGCAGAGCATGGAGGGAAGAGGATTTTTATAAGAGAAATTTCTGCACTTT
 161 GAAACTGTCCTCTAAGAGAATAAGCATTTCCTGTTCTTCCAGCTCCAGGTCCACCTCCTGTTGGGAGGCGGTGGGGGGCC
 241 AAAGTGGGGCCACACACTCGCTGTGTCCCCTCTCCTCCCCTGTGCCAGTGCCACCTGGGTGCCTCCTCCTGTCCTGTCCG
 321 TCTCAACCTCCCTCCCGTCCAGCATTGAGTGTGTACATGTGTGTGTGACACATAAATATACTCATAAGGACACCTCC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' uaACCCAAAAUUCUCAAGAAAu 5'
            ||||   |   ||| |||| 
Target 5' tcTGGGCCCTTCCAGTGCTTTg 3'
11 - 32 120.00 -8.54
2
miRNA  3' uaaCCCAAAAUUCU-CAAGAAAu 5'
             |||    |||| | |:||| 
Target 5' ggaGGG----AAGAGGATTTTTa 3'
121 - 139 105.00 -9.10
3
miRNA  3' uaacccaaaauucuCAAgaaau 5'
                        |||     
Target 5' caggtccacctcctGTTgggag 3'
206 - 227 60.00 -7.41
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30191314 15 COSMIC
COSN30479595 20 COSMIC
COSN26987572 40 COSMIC
COSN13374427 41 COSMIC
COSN30517185 67 COSMIC
COSN26987574 87 COSMIC
COSN30108558 95 COSMIC
COSN19632158 120 COSMIC
COSN20110239 139 COSMIC
COSN28793406 144 COSMIC
COSN31591485 153 COSMIC
COSN5713290 214 COSMIC
COSN1143509 390 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs782539967 3 dbSNP
rs782672437 5 dbSNP
rs782202819 6 dbSNP
rs1450750500 7 dbSNP
rs782317329 11 dbSNP
rs1341623906 22 dbSNP
rs1328822457 23 dbSNP
rs1408560244 25 dbSNP
rs782617012 33 dbSNP
rs368266882 35 dbSNP
rs1171572265 38 dbSNP
rs1461134919 39 dbSNP
rs559471213 40 dbSNP
rs782165393 41 dbSNP
rs1473191920 48 dbSNP
rs782407788 49 dbSNP
rs11548651 51 dbSNP
rs991605346 54 dbSNP
rs1023124875 65 dbSNP
rs544943023 67 dbSNP
rs982103461 68 dbSNP
rs782717156 70 dbSNP
rs563241286 76 dbSNP
rs908168808 77 dbSNP
rs782478951 85 dbSNP
rs1228364256 86 dbSNP
rs76753792 87 dbSNP
rs548927860 94 dbSNP
rs1440652606 95 dbSNP
rs932417665 101 dbSNP
rs1379502744 113 dbSNP
rs1332487985 115 dbSNP
rs567672699 119 dbSNP
rs712853 120 dbSNP
rs1392511929 130 dbSNP
rs35558190 134 dbSNP
rs782647878 135 dbSNP
rs546759203 138 dbSNP
rs912404568 140 dbSNP
rs782671184 142 dbSNP
rs943937766 144 dbSNP
rs1441484631 155 dbSNP
rs112873263 158 dbSNP
rs1192810627 161 dbSNP
rs903160496 173 dbSNP
rs1248072284 179 dbSNP
rs73227930 186 dbSNP
rs1445850692 191 dbSNP
rs1281542372 193 dbSNP
rs1238545331 194 dbSNP
rs1356502831 198 dbSNP
rs117741979 207 dbSNP
rs143888039 222 dbSNP
rs893215312 224 dbSNP
rs34428119 226 dbSNP
rs376038517 230 dbSNP
rs1365997478 241 dbSNP
rs35143334 241 dbSNP
rs537053154 244 dbSNP
rs183557346 250 dbSNP
rs1437467511 258 dbSNP
rs1344628615 260 dbSNP
rs782288402 260 dbSNP
rs1012993489 261 dbSNP
rs1380809505 268 dbSNP
rs1172989085 271 dbSNP
rs1023049552 273 dbSNP
rs1191355094 276 dbSNP
rs1474889599 279 dbSNP
rs1239819244 281 dbSNP
rs1218122704 283 dbSNP
rs146865378 286 dbSNP
rs1488358346 292 dbSNP
rs1287348809 294 dbSNP
rs1210875308 295 dbSNP
rs1310746326 305 dbSNP
rs1303886043 310 dbSNP
rs971979950 320 dbSNP
rs782255248 321 dbSNP
rs1276590302 327 dbSNP
rs1401901112 328 dbSNP
rs1361316403 333 dbSNP
rs1312195516 337 dbSNP
rs552929411 338 dbSNP
rs75886182 346 dbSNP
rs782673796 354 dbSNP
rs138241823 357 dbSNP
rs1409194964 357 dbSNP
rs1195467644 378 dbSNP
rs1477857092 380 dbSNP
rs774657981 381 dbSNP
rs961672627 386 dbSNP
rs1482577180 387 dbSNP
rs1258095761 395 dbSNP
rs1211226076 398 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084080. RNA binding protein: AGO2. Condition:CLIP_nohippuristanol_rep2_SantaCruzAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
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174 hsa-miR-3133 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT057804 SLC30A7 solute carrier family 30 member 7 2 2
MIRT075401 ZFHX3 zinc finger homeobox 3 2 2
MIRT088350 MAPRE3 microtubule associated protein RP/EB family member 3 2 6
MIRT107288 FAM73B mitoguardin 2 2 2
MIRT141571 ABHD2 abhydrolase domain containing 2 2 2
MIRT161036 CDV3 CDV3 homolog 2 2
MIRT167732 RNF146 ring finger protein 146 8 3
MIRT209559 PAK2 p21 (RAC1) activated kinase 2 2 2
MIRT214631 SMAD5 SMAD family member 5 2 8
MIRT258393 KIAA0895 KIAA0895 2 2
MIRT272744 ALG10B ALG10B, alpha-1,2-glucosyltransferase 2 2
MIRT309347 SMARCA5 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 2 6
MIRT329705 SCD stearoyl-CoA desaturase 2 2
MIRT336556 SDE2 SDE2 telomere maintenance homolog 2 2
MIRT363351 AGO2 argonaute 2, RISC catalytic component 2 4
MIRT386753 TAOK1 TAO kinase 1 2 2
MIRT441580 EXOC5 exocyst complex component 5 2 2
MIRT441735 SEPT8 septin 8 2 2
MIRT441817 LRRC40 leucine rich repeat containing 40 2 2
MIRT442180 TRIM59 tripartite motif containing 59 2 4
MIRT442277 TLX3 T-cell leukemia homeobox 3 2 2
MIRT442489 TBC1D19 TBC1 domain family member 19 2 2
MIRT443242 ANKRD26 ankyrin repeat domain 26 2 2
MIRT443270 ZWINT ZW10 interacting kinetochore protein 2 2
MIRT444114 CEPT1 choline/ethanolamine phosphotransferase 1 2 2
MIRT444725 LAMA2 laminin subunit alpha 2 2 2
MIRT445346 FAM169A family with sequence similarity 169 member A 2 2
MIRT445693 GJB1 gap junction protein beta 1 2 2
MIRT446051 CDC73 cell division cycle 73 2 2
MIRT447746 TMCC3 transmembrane and coiled-coil domain family 3 2 2
MIRT447955 AKR7A2 aldo-keto reductase family 7 member A2 2 2
MIRT449199 ZNF623 zinc finger protein 623 2 2
MIRT449564 GPC5 glypican 5 2 2
MIRT450938 BDP1 B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB 2 2
MIRT459811 POTED POTE ankyrin domain family member D 2 8
MIRT461692 ZNF426 zinc finger protein 426 2 2
MIRT462014 SNRNP27 small nuclear ribonucleoprotein U4/U6.U5 subunit 27 2 2
MIRT465150 TSC22D2 TSC22 domain family member 2 2 2
MIRT467144 SREK1IP1 SREK1 interacting protein 1 2 2
MIRT474929 KCTD15 potassium channel tetramerization domain containing 15 2 8
MIRT475118 IPPK inositol-pentakisphosphate 2-kinase 2 2
MIRT476844 FIGN fidgetin, microtubule severing factor 2 10
MIRT477336 EPHA2 EPH receptor A2 2 2
MIRT477853 DYRK3 dual specificity tyrosine phosphorylation regulated kinase 3 2 2
MIRT481113 B2M beta-2-microglobulin 2 2
MIRT481296 ATP6V1B2 ATPase H+ transporting V1 subunit B2 2 10
MIRT485094 SLC30A1 solute carrier family 30 member 1 2 4
MIRT491908 YWHAE tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon 2 2
MIRT492458 RBPJ recombination signal binding protein for immunoglobulin kappa J region 2 8
MIRT493341 LAPTM4A lysosomal protein transmembrane 4 alpha 2 12
MIRT494771 AP1G1 adaptor related protein complex 1 gamma 1 subunit 2 2
MIRT497837 GPR26 G protein-coupled receptor 26 2 2
MIRT497958 DGAT2 diacylglycerol O-acyltransferase 2 2 2
MIRT498445 INO80D INO80 complex subunit D 2 2
MIRT498997 HAT1 histone acetyltransferase 1 2 10
MIRT499257 VAV3 vav guanine nucleotide exchange factor 3 2 4
MIRT500067 SFT2D2 SFT2 domain containing 2 2 4
MIRT501385 RBFOX2 RNA binding protein, fox-1 homolog 2 2 10
MIRT503084 BTG2 BTG anti-proliferation factor 2 2 4
MIRT503272 SLC7A5 solute carrier family 7 member 5 2 2
MIRT504341 ASGR2 asialoglycoprotein receptor 2 2 2
MIRT505453 SUGT1 SGT1 homolog, MIS12 kinetochore complex assembly cochaperone 2 6
MIRT505905 RIMS3 regulating synaptic membrane exocytosis 3 2 6
MIRT507917 C7orf60 base methyltransferase of 25S rRNA 2 homolog 2 6
MIRT508174 FRK fyn related Src family tyrosine kinase 2 4
MIRT508287 YES1 YES proto-oncogene 1, Src family tyrosine kinase 2 4
MIRT509232 KIF14 kinesin family member 14 2 6
MIRT509481 GNL3L G protein nucleolar 3 like 2 6
MIRT509530 INTU inturned planar cell polarity protein 2 6
MIRT509548 BTLA B and T lymphocyte associated 2 8
MIRT511328 KIAA1522 KIAA1522 2 4
MIRT511427 HSP90AA1 heat shock protein 90 alpha family class A member 1 2 4
MIRT511477 HNRNPC heterogeneous nuclear ribonucleoprotein C (C1/C2) 2 4
MIRT512471 CCDC149 coiled-coil domain containing 149 2 2
MIRT512996 SEZ6L seizure related 6 homolog like 2 2
MIRT514755 ACER3 alkaline ceramidase 3 2 8
MIRT515764 KRTAP5-6 keratin associated protein 5-6 2 2
MIRT517392 TBK1 TANK binding kinase 1 2 4
MIRT518720 FTH1 ferritin heavy chain 1 2 2
MIRT521923 PHIP pleckstrin homology domain interacting protein 2 4
MIRT522849 KIAA2022 neurite extension and migration factor 2 4
MIRT522887 KDELR1 KDEL endoplasmic reticulum protein retention receptor 1 2 2
MIRT523153 HNRNPU heterogeneous nuclear ribonucleoprotein U 2 2
MIRT525149 ZNF256 zinc finger protein 256 2 2
MIRT525941 C11orf74 chromosome 11 open reading frame 74 2 2
MIRT526876 ERCC8 ERCC excision repair 8, CSA ubiquitin ligase complex subunit 2 2
MIRT527366 KRTAP13-2 keratin associated protein 13-2 2 2
MIRT527549 DLGAP5 DLG associated protein 5 2 4
MIRT527604 ANKRD42 ankyrin repeat domain 42 2 2
MIRT527803 KLRD1 killer cell lectin like receptor D1 2 2
MIRT528046 PRKD3 protein kinase D3 2 2
MIRT528397 PGK1 phosphoglycerate kinase 1 2 2
MIRT529673 SLC28A1 solute carrier family 28 member 1 2 2
MIRT530358 HPSE heparanase 2 2
MIRT530908 KIAA1161 myogenesis regulating glycosidase (putative) 2 2
MIRT531169 ZNF333 zinc finger protein 333 2 2
MIRT531438 PAK1 p21 (RAC1) activated kinase 1 2 2
MIRT531562 CENPC1P1 centromere protein C pseudogene 1 1 1
MIRT532783 LDHD lactate dehydrogenase D 2 2
MIRT532848 ZNF699 zinc finger protein 699 2 2
MIRT534287 SLC12A7 solute carrier family 12 member 7 2 2
MIRT535078 PPIG peptidylprolyl isomerase G 2 4
MIRT535968 MFAP3L microfibril associated protein 3 like 2 2
MIRT536163 MAP3K9 mitogen-activated protein kinase kinase kinase 9 2 4
MIRT537417 FBXO47 F-box protein 47 2 2
MIRT538665 CCSAP centriole, cilia and spindle associated protein 2 2
MIRT540291 RGR retinal G protein coupled receptor 2 4
MIRT543332 KIAA1456 KIAA1456 2 2
MIRT543392 CC2D2A coiled-coil and C2 domain containing 2A 2 2
MIRT543891 AIMP1 aminoacyl tRNA synthetase complex interacting multifunctional protein 1 2 2
MIRT545609 SIGLEC9 sialic acid binding Ig like lectin 9 2 2
MIRT545839 ZNF275 zinc finger protein 275 2 2
MIRT546689 RORA RAR related orphan receptor A 2 4
MIRT547513 MAT2A methionine adenosyltransferase 2A 2 4
MIRT549431 ACVR1 activin A receptor type 1 2 2
MIRT550567 MYC MYC proto-oncogene, bHLH transcription factor 2 4
MIRT552517 ZIC5 Zic family member 5 2 2
MIRT552743 YRDC yrdC N6-threonylcarbamoyltransferase domain containing 2 2
MIRT552942 VKORC1L1 vitamin K epoxide reductase complex subunit 1 like 1 2 2
MIRT555752 PDCD10 programmed cell death 10 2 2
MIRT558002 FAM160B1 family with sequence similarity 160 member B1 2 2
MIRT559422 ASF1B anti-silencing function 1B histone chaperone 2 2
MIRT560276 SF1 splicing factor 1 2 2
MIRT560713 ZNF324B zinc finger protein 324B 2 2
MIRT561190 TNFRSF10A TNF receptor superfamily member 10a 2 2
MIRT561817 NUP50 nucleoporin 50 2 2
MIRT562018 LCLAT1 lysocardiolipin acyltransferase 1 2 2
MIRT562847 LINC00346 long intergenic non-protein coding RNA 346 2 2
MIRT563252 SETD6 SET domain containing 6 2 2
MIRT563978 GCC2 GRIP and coiled-coil domain containing 2 2 2
MIRT564383 BAAT bile acid-CoA:amino acid N-acyltransferase 2 2
MIRT566552 PANK3 pantothenate kinase 3 2 2
MIRT566933 LIN28B lin-28 homolog B 2 2
MIRT566989 LBR lamin B receptor 2 2
MIRT567645 EXT1 exostosin glycosyltransferase 1 2 2
MIRT568268 BICD2 BICD cargo adaptor 2 2 2
MIRT571407 MED4 mediator complex subunit 4 2 2
MIRT571784 PPP2CA protein phosphatase 2 catalytic subunit alpha 2 2
MIRT572437 ATM ATM serine/threonine kinase 2 2
MIRT573613 COX6A1P2 cytochrome c oxidase subunit 6A1 pseudogene 2 2 2
MIRT574289 COX6A1 cytochrome c oxidase subunit 6A1 2 2
MIRT574375 YY1 YY1 transcription factor 2 2
MIRT613142 DSEL dermatan sulfate epimerase-like 2 2
MIRT616661 ORAI1 ORAI calcium release-activated calcium modulator 1 2 2
MIRT619345 SASH1 SAM and SH3 domain containing 1 2 2
MIRT626315 ABCC9 ATP binding cassette subfamily C member 9 2 2
MIRT628235 FAM117B family with sequence similarity 117 member B 2 2
MIRT630452 GTPBP8 GTP binding protein 8 (putative) 2 2
MIRT631013 TMEM30B transmembrane protein 30B 2 2
MIRT637315 FBXO18 F-box protein, helicase, 18 2 2
MIRT640658 FRY FRY microtubule binding protein 2 2
MIRT642081 FUT11 fucosyltransferase 11 2 2
MIRT644607 C17orf77 chromosome 17 open reading frame 77 2 2
MIRT644829 DNAJC21 DnaJ heat shock protein family (Hsp40) member C21 2 2
MIRT652465 TMEM2 transmembrane protein 2 2 2
MIRT657514 HAS2 hyaluronan synthase 2 2 2
MIRT658290 FAM86C1 family with sequence similarity 86 member C1 2 2
MIRT659570 CGGBP1 CGG triplet repeat binding protein 1 2 2
MIRT675457 SRP19 signal recognition particle 19 2 2
MIRT687828 ITGB8 integrin subunit beta 8 2 2
MIRT693550 MYO16 myosin XVI 2 2
MIRT697294 ZNF654 zinc finger protein 654 2 2
MIRT699804 SEC24A SEC24 homolog A, COPII coat complex component 2 2
MIRT704867 CD164 CD164 molecule 2 2
MIRT705309 AVL9 AVL9 cell migration associated 2 2
MIRT705323 ATP2A2 ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 2 2
MIRT707180 HOXC4 homeobox C4 2 2
MIRT709331 CLEC12B C-type lectin domain family 12 member B 2 2
MIRT712321 PER2 period circadian clock 2 2 2
MIRT713817 XRCC2 X-ray repair cross complementing 2 2 2
MIRT713854 HHLA2 HERV-H LTR-associating 2 2 2
MIRT717257 SLC44A1 solute carrier family 44 member 1 2 2
MIRT719956 BLOC1S6 biogenesis of lysosomal organelles complex 1 subunit 6 2 2
MIRT725408 KIF6 kinesin family member 6 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-3133 5-Fluorouracil approved 3385 Microarray CNE cells 22614822 2012 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-3133 Imatinib 5291 NSC743414 approved sensitive High Gastrointestinal Stromal Tumor cell line (882R-NC, 882R-OE, 882R-KD)
hsa-mir-3133 Androstenedione+Anastrozole sensitive cell line (MCF-7)
hsa-mir-3133 Cisplatin 5460033 NSC119875 approved sensitive cell line (BxPC3)
hsa-miR-3133 Gefitinib 123631 NSC715055 approved sensitive cell line (PC9)
hsa-miR-3133 Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-3133 Osimertinib 71496458 NSC779217 approved resistant cell line (HCC827)
hsa-miR-3133 Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-3133 Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-3133 Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (1500 ng/ml)

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