pre-miRNA Information
pre-miRNA hsa-mir-3622b   
Genomic Coordinates chr8: 27701673 - 27701767
Description Homo sapiens miR-3622b stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-3622b-3p
Sequence 58| UCACCUGAGCUCCCGUGCCUG |78
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1232922758 2 dbSNP
rs530939348 5 dbSNP
rs1439657384 10 dbSNP
rs1157333896 12 dbSNP
rs66683138 14 dbSNP
rs1044808718 15 dbSNP
rs946438979 19 dbSNP
rs551845360 21 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol NTMT1   
Synonyms AD-003, C9orf32, HOMT1A, METTL11A, NRMT, NRMT1, NTM1A
Description N-terminal Xaa-Pro-Lys N-methyltransferase 1
Transcript NM_014064   
Expression
Putative miRNA Targets on NTMT1
3'UTR of NTMT1
(miRNA target sites are highlighted)
>NTMT1|NM_014064|3'UTR
   1 GCCGGGGCTGGCAGGAGAAACTGAGGAACCACAGTCCTGGTGGGGGGAGCTGGCAGCTGGGCAAGATCCAGGCGCCACGC
  81 TGGCGGTTCGTGAGTGTCGAGGCACCACTAAATATAGCTGTCTGCCGTCCACTCATTATGCGGGCTCTTCTTCAAAAGGC
 161 AAGGTGGGACCCGGCGGGGAGGGTGCTGCTGAACCAGCGGTGAGGCAGGAGCCCAGACCCTGCTCTCCTGCGAGATGGGA
 241 TTGGGTGGAAGGGGCTCCAGGGCTCCTGGTGCTCCCCATGTGGGAATAGGGTGGCCACATCAGTAACCGATTCCCTGGGC
 321 CTCCAGCCCGGCCTTCCAGCCATGGGCCTGGCTGCCTGAAGAGGAAGGAAACCACCCCCAACTTGGTTGCTGGGGACTTG
 401 AAGACTTCAGTGTGATCTTTACCTAGGGGAGGGACAGAGCTGGGCTGGTGGAGCCCATGGGTGCTGCCTGATGGTGCTGT
 481 GGGGTGGGTGCTCATGTGCCAAGTCCTGCTTGGCAGTGGAGACCTGGGGCCCTTAAAGTGTTACAACAGCCTGCAGCAAG
 561 GGAAGTTCCCCGTAGCCTCAGTCTTCTCTGGGCTTGAGGCTGCTCCTTGGCAGGCATTTAAAGTAAAAAATAAAATGGGG
 641 CTGGGCGCGGTGGCTCACGCCTGTAAT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' guCCGUGCCCUCGAGUCCACu 5'
            || ::||| ||||| ||| 
Target 5' gtGGGGTGGGTGCTCATGTGc 3'
479 - 499 139.00 -21.40
2
miRNA  3' guccgUGCCCUCGAGUCCACu 5'
               |:||||  |::|||| 
Target 5' gcgagATGGGA--TTGGGTGg 3'
230 - 248 124.00 -16.60
3
miRNA  3' guCCGUG--C-CCUCGAGUC-CACu 5'
            || ||  |  || :|||| ||| 
Target 5' tgGGGACTTGAAGACTTCAGTGTGa 3'
391 - 415 117.00 -14.30
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30177617 5 COSMIC
COSN30191708 8 COSMIC
COSN30582166 18 COSMIC
COSN31579965 23 COSMIC
COSN26552679 43 COSMIC
COSN26987119 48 COSMIC
COSN30492831 48 COSMIC
COSN30535493 76 COSMIC
COSN14375453 86 COSMIC
COSN26987118 87 COSMIC
COSN30529925 102 COSMIC
COSN19564835 114 COSMIC
COSN31611025 125 COSMIC
COSN30670780 142 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1265496702 1 dbSNP
rs769513261 2 dbSNP
rs762457236 3 dbSNP
rs55943174 4 dbSNP
rs369469508 5 dbSNP
rs761847792 6 dbSNP
rs767314125 7 dbSNP
rs541925695 19 dbSNP
rs755807015 20 dbSNP
rs769251299 25 dbSNP
rs766628583 27 dbSNP
rs1391223609 31 dbSNP
rs1435218431 33 dbSNP
rs1307575853 34 dbSNP
rs935517544 36 dbSNP
rs372578515 37 dbSNP
rs1241168790 38 dbSNP
rs1279800812 39 dbSNP
rs755236847 40 dbSNP
rs1252425430 41 dbSNP
rs1228943074 42 dbSNP
rs775408144 42 dbSNP
rs779185577 43 dbSNP
rs748210162 44 dbSNP
rs758976003 45 dbSNP
rs1239787007 47 dbSNP
rs1440004005 48 dbSNP
rs1328964614 57 dbSNP
rs939123509 66 dbSNP
rs1397099232 69 dbSNP
rs1169621485 70 dbSNP
rs78602607 74 dbSNP
rs1039720016 75 dbSNP
rs1423258396 79 dbSNP
rs901194971 80 dbSNP
rs1454826417 81 dbSNP
rs1402832566 83 dbSNP
rs997440678 85 dbSNP
rs1052149975 86 dbSNP
rs541975307 90 dbSNP
rs183816323 91 dbSNP
rs1481980716 100 dbSNP
rs1015945357 102 dbSNP
rs1044030645 103 dbSNP
rs963112557 114 dbSNP
rs1012436994 115 dbSNP
rs1349540502 121 dbSNP
rs867993043 127 dbSNP
rs1239124370 132 dbSNP
rs1436486837 135 dbSNP
rs970837220 136 dbSNP
rs982375852 142 dbSNP
rs891169556 143 dbSNP
rs1008277255 146 dbSNP
rs1312314091 160 dbSNP
rs187872989 161 dbSNP
rs924197677 173 dbSNP
rs1397090994 174 dbSNP
rs1273890923 176 dbSNP
rs35274982 176 dbSNP
rs550458768 177 dbSNP
rs1184441954 180 dbSNP
rs1422513585 182 dbSNP
rs956819227 187 dbSNP
rs1210952061 189 dbSNP
rs1486389615 192 dbSNP
rs762917352 199 dbSNP
rs1204242869 200 dbSNP
rs373184580 205 dbSNP
rs532750590 210 dbSNP
rs546602332 212 dbSNP
rs996043241 217 dbSNP
rs1028026423 231 dbSNP
rs951999561 232 dbSNP
rs1040233761 233 dbSNP
rs983655806 235 dbSNP
rs1385948684 238 dbSNP
rs1160616226 246 dbSNP
rs1456757679 251 dbSNP
rs1417737064 252 dbSNP
rs566366093 254 dbSNP
rs1014774460 260 dbSNP
rs535496886 267 dbSNP
rs758733240 269 dbSNP
rs778008792 281 dbSNP
rs886378409 285 dbSNP
rs745498374 286 dbSNP
rs774392110 291 dbSNP
rs555178296 292 dbSNP
rs1273731713 296 dbSNP
rs1037485577 298 dbSNP
rs979678046 309 dbSNP
rs145444758 310 dbSNP
rs1327194747 314 dbSNP
rs1320579578 321 dbSNP
rs1011994369 324 dbSNP
rs935602332 325 dbSNP
rs1328828065 327 dbSNP
rs1023837498 328 dbSNP
rs2270402 330 dbSNP
rs557674962 331 dbSNP
rs1031234693 333 dbSNP
rs1349656145 339 dbSNP
rs1226284813 343 dbSNP
rs774962206 344 dbSNP
rs995430414 347 dbSNP
rs1467855722 349 dbSNP
rs1253329800 353 dbSNP
rs1215337773 373 dbSNP
rs989758130 377 dbSNP
rs1315941971 378 dbSNP
rs1026995469 387 dbSNP
rs753630070 391 dbSNP
rs1004821839 393 dbSNP
rs1340229415 395 dbSNP
rs1272133130 397 dbSNP
rs746909615 398 dbSNP
rs1015152359 406 dbSNP
rs1370540957 410 dbSNP
rs1183248448 423 dbSNP
rs964595152 434 dbSNP
rs960557897 436 dbSNP
rs975490258 443 dbSNP
rs991899614 449 dbSNP
rs1328817755 451 dbSNP
rs922682957 454 dbSNP
rs933985227 458 dbSNP
rs1011880874 460 dbSNP
rs1471820583 477 dbSNP
rs139412944 478 dbSNP
rs908580697 485 dbSNP
rs1453348997 493 dbSNP
rs940591822 496 dbSNP
rs1419245265 503 dbSNP
rs1472787895 504 dbSNP
rs1456312762 512 dbSNP
rs1205207360 513 dbSNP
rs979471299 519 dbSNP
rs1232225711 535 dbSNP
rs1347369308 536 dbSNP
rs1161967472 540 dbSNP
rs1236919652 543 dbSNP
rs924983133 545 dbSNP
rs1356790318 547 dbSNP
rs1037620263 548 dbSNP
rs1310499196 554 dbSNP
rs527281940 555 dbSNP
rs947885127 556 dbSNP
rs1175169380 560 dbSNP
rs758881383 561 dbSNP
rs1406903213 570 dbSNP
rs759529651 572 dbSNP
rs546203629 573 dbSNP
rs1235688904 577 dbSNP
rs1294949030 586 dbSNP
rs1340819168 590 dbSNP
rs1030658369 595 dbSNP
rs892176069 599 dbSNP
rs1011156313 607 dbSNP
rs1306650108 612 dbSNP
rs553300288 617 dbSNP
rs573166243 621 dbSNP
rs1341562371 625 dbSNP
rs976007332 632 dbSNP
rs1205887889 637 dbSNP
rs1029720431 644 dbSNP
rs1482172890 647 dbSNP
rs955631716 648 dbSNP
rs1314191339 649 dbSNP
rs931411215 655 dbSNP
rs796948906 657 dbSNP
rs1451093808 658 dbSNP
rs542263406 659 dbSNP
rs1048851279 668 dbSNP
rs1213245408 671 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084079. RNA binding protein: AGO2. Condition:CLIP_hippuristanol_rep2_AbnovaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' guccgugcccucgaGUCCACu 5'
                        |||||| 
Target 5' -------------aCAGGUGa 3'
1 - 8
Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084079
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_hippuristanol_rep2_AbnovaAb
Location of target site ENST00000372481.3 | 3UTR | ACAGGUGAAACCCCGUCUCUACU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
102 hsa-miR-3622b-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT076713 NUFIP2 NUFIP2, FMR1 interacting protein 2 2 2
MIRT080212 PRKACB protein kinase cAMP-activated catalytic subunit beta 2 2
MIRT081398 GTPBP3 GTP binding protein 3, mitochondrial 2 2
MIRT107158 ZBTB43 zinc finger and BTB domain containing 43 2 2
MIRT254071 BACH1 BTB domain and CNC homolog 1 2 2
MIRT409790 FOXO3 forkhead box O3 2 2
MIRT448679 MAPK9 mitogen-activated protein kinase 9 2 2
MIRT450206 ABHD15 abhydrolase domain containing 15 2 2
MIRT486621 PDK3 pyruvate dehydrogenase kinase 3 2 2
MIRT489267 TTLL1 tubulin tyrosine ligase like 1 2 2
MIRT493117 MKNK2 MAP kinase interacting serine/threonine kinase 2 2 4
MIRT494570 BAK1 BCL2 antagonist/killer 1 2 2
MIRT497395 RALY RALY heterogeneous nuclear ribonucleoprotein 2 2
MIRT504900 CCDC86 coiled-coil domain containing 86 2 2
MIRT505192 USP46 ubiquitin specific peptidase 46 2 4
MIRT506522 MSANTD4 Myb/SANT DNA binding domain containing 4 with coiled-coils 2 6
MIRT508162 ABCC5 ATP binding cassette subfamily C member 5 2 8
MIRT508545 PARVG parvin gamma 2 4
MIRT509850 BIRC5 baculoviral IAP repeat containing 5 2 6
MIRT510088 PPWD1 peptidylprolyl isomerase domain and WD repeat containing 1 2 8
MIRT512094 CRK CRK proto-oncogene, adaptor protein 2 4
MIRT515381 RPL7 ribosomal protein L7 2 2
MIRT519176 SCO1 SCO1, cytochrome c oxidase assembly protein 2 2
MIRT519964 ZCCHC8 zinc finger CCHC-type containing 8 2 2
MIRT522292 NKAP NFKB activating protein 2 2
MIRT523160 HMGB2 high mobility group box 2 2 4
MIRT525314 FANCA Fanconi anemia complementation group A 2 2
MIRT528194 PLEKHM2 pleckstrin homology and RUN domain containing M2 2 2
MIRT532885 ZNF451 zinc finger protein 451 2 2
MIRT538209 CYR61 cysteine rich angiogenic inducer 61 2 2
MIRT539497 ACTN4 actinin alpha 4 2 2
MIRT554600 RRAGC Ras related GTP binding C 2 2
MIRT562444 DCTN6 dynactin subunit 6 2 2
MIRT562748 AOC3 amine oxidase, copper containing 3 2 2
MIRT565798 SEC14L5 SEC14 like lipid binding 5 2 2
MIRT565838 SCML2 Scm polycomb group protein like 2 2 2
MIRT566072 RCC2 regulator of chromosome condensation 2 2 2
MIRT566104 RBPJ recombination signal binding protein for immunoglobulin kappa J region 2 2
MIRT572407 MRPS14 mitochondrial ribosomal protein S14 2 2
MIRT576198 Vsig2 V-set and immunoglobulin domain containing 2 2 2
MIRT576313 Acbd7 acyl-Coenzyme A binding domain containing 7 2 2
MIRT576651 Mill2 MHC I like leukocyte 2 1 1
MIRT576859 Socs6 suppressor of cytokine signaling 6 2 2
MIRT606819 BICD2 BICD cargo adaptor 2 2 2
MIRT610738 NUDT16 nudix hydrolase 16 2 4
MIRT614798 RORA RAR related orphan receptor A 2 2
MIRT619006 NTMT1 N-terminal Xaa-Pro-Lys N-methyltransferase 1 2 2
MIRT619420 NOS1AP nitric oxide synthase 1 adaptor protein 2 2
MIRT620405 MYO1H myosin IH 2 2
MIRT624869 ABHD13 abhydrolase domain containing 13 2 2
MIRT633527 ZFP30 ZFP30 zinc finger protein 2 2
MIRT633927 DNAH9 dynein axonemal heavy chain 9 2 2
MIRT636611 CLIC5 chloride intracellular channel 5 2 2
MIRT640616 MIOX myo-inositol oxygenase 2 2
MIRT642605 C14orf180 chromosome 14 open reading frame 180 2 2
MIRT644624 SRSF2 serine and arginine rich splicing factor 2 2 2
MIRT655113 PHLDA3 pleckstrin homology like domain family A member 3 2 2
MIRT658976 DNAJB5 DnaJ heat shock protein family (Hsp40) member B5 2 4
MIRT661495 CHMP1B charged multivesicular body protein 1B 2 2
MIRT662996 TMEM59 transmembrane protein 59 2 2
MIRT663075 SFR1 SWI5 dependent homologous recombination repair protein 1 2 2
MIRT665405 WEE1 WEE1 G2 checkpoint kinase 2 2
MIRT666106 SSR1 signal sequence receptor subunit 1 2 2
MIRT669562 ALDOA aldolase, fructose-bisphosphate A 2 2
MIRT670070 ZNF783 zinc finger family member 783 2 2
MIRT671192 ZNF891 zinc finger protein 891 2 2
MIRT675361 KLHL26 kelch like family member 26 2 2
MIRT678857 LINC00598 long intergenic non-protein coding RNA 598 2 2
MIRT679443 C19orf52 translocase of inner mitochondrial membrane 29 2 2
MIRT684163 ALDH1B1 aldehyde dehydrogenase 1 family member B1 2 2
MIRT684543 ZNF460 zinc finger protein 460 2 2
MIRT684674 SLC2A11 solute carrier family 2 member 11 2 2
MIRT684971 MINOS1 mitochondrial inner membrane organizing system 1 2 2
MIRT686006 NEK4 NIMA related kinase 4 2 2
MIRT687758 KIAA1328 KIAA1328 2 2
MIRT688961 ATXN3 ataxin 3 2 2
MIRT689409 UQCR11 ubiquinol-cytochrome c reductase, complex III subunit XI 2 2
MIRT690001 MMP17 matrix metallopeptidase 17 2 2
MIRT690165 ELP3 elongator acetyltransferase complex subunit 3 2 2
MIRT690202 C5orf45 MRN complex interacting protein 2 2
MIRT690477 ZNF33A zinc finger protein 33A 2 2
MIRT690566 MICA MHC class I polypeptide-related sequence A 2 2
MIRT692160 C10orf111 chromosome 10 open reading frame 111 2 2
MIRT693430 PLGLB2 plasminogen-like B2 2 2
MIRT693547 ZNF708 zinc finger protein 708 2 2
MIRT693697 PLGLB1 plasminogen-like B1 2 2
MIRT695006 HSPA6 heat shock protein family A (Hsp70) member 6 2 2
MIRT698525 TFRC transferrin receptor 2 2
MIRT699574 SIKE1 suppressor of IKBKE 1 2 2
MIRT703446 FYTTD1 forty-two-three domain containing 1 2 2
MIRT704397 CTSS cathepsin S 2 2
MIRT704485 CPT1A carnitine palmitoyltransferase 1A 2 2
MIRT709822 STPG1 sperm tail PG-rich repeat containing 1 2 2
MIRT710859 COQ7 coenzyme Q7, hydroxylase 2 2
MIRT711893 INSIG2 insulin induced gene 2 2 2
MIRT713796 CPLX2 complexin 2 2 2
MIRT718800 C1GALT1C1 C1GALT1 specific chaperone 1 2 2
MIRT719501 SEC24B SEC24 homolog B, COPII coat complex component 2 2
MIRT719722 PDE6B phosphodiesterase 6B 2 2
MIRT722204 URM1 ubiquitin related modifier 1 2 2
MIRT723523 CLPTM1L CLPTM1 like 2 2
MIRT725471 GRAP2 GRB2-related adaptor protein 2 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-3622b-3p Platinum 23939 sensitive tissue
hsa-miR-3622b-3p Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-3622b-3p Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (1500 ng/ml)
hsa-miR-3622b-3p Paclitaxel/Docetaxel/Vinorelbine/Doxorubicin/Etoposide resistant cell line (Bads-200)

Error report submission