pre-miRNA Information | |
---|---|
pre-miRNA | hsa-mir-3622b |
Genomic Coordinates | chr8: 27701673 - 27701767 |
Description | Homo sapiens miR-3622b stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Mature miRNA Information | ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mature miRNA | hsa-miR-3622b-3p | |||||||||||||||||||||||||||
Sequence | 58| UCACCUGAGCUCCCGUGCCUG |78 | |||||||||||||||||||||||||||
Evidence | Experimental | |||||||||||||||||||||||||||
Experiments | Illumina | |||||||||||||||||||||||||||
SNPs in miRNA |
|
|||||||||||||||||||||||||||
Putative Targets |
miRNA Expression profile | |
---|---|
Human miRNA Tissue Atlas | |
Circulating MicroRNA Expression Profiling |
Gene Information | |||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Gene Symbol | MYO1H | ||||||||||||||||||||
Synonyms | - | ||||||||||||||||||||
Description | myosin IH | ||||||||||||||||||||
Transcript | NM_001101421 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on MYO1H | |||||||||||||||||||||
3'UTR of MYO1H (miRNA target sites are highlighted) |
>MYO1H|NM_001101421|3'UTR 1 TAGAGGATGACGTCTGACCTCTACCATCGCCATTTTTGCTCCAACTGAGGAAACTACAGGGGAAGTGGGGATTGGATCCA 81 GTTAGCTACCTCTTCAAGGTACCAGGCCCGCAGCACTAACAGATCACATCTGAAGAAACTGAGGGGCGTTAGGCCAAAGC 161 CTAATCCCAGCTCCTCAGCTGCCCCATGGCACCTTATTTAAAATGTCACCTTCTGGAAGCAGACCCCAGGTCACCCGAAG 241 GCGCAATTCTAAACACCCTCGACAGGATCTATGTTCAGTGCGCAGTCCAACGTGTCATTAGAGGGTGGGCAAGATTCTCT 321 ACCTCTGTCAAGCTGCCCTGAGACATGATGAAATACTGTGTACAGCAAGTTCTCAAATAACATTTCGTTATATGTTGATA 401 AGACATCTGATTCCTGGGCAGGGCCACTGTCTGGGTGGAGTCTGTATGTTCTCCCCATGTCTCTGGGTTTCTTCTGGGTG 481 CTCTGGTTTCCTCCCACGTCCCCAAGGTGTGTGTGTCAGGTGCACTGGCGTGTCTATGCGGTCCCAGTCTGAGTGAGTGT 561 GGGTGCGTGAGCCCCGTGAGAGGAGGACATCCTGTCCAGGGTGGGTCCCGCCTTCTGCCCTGAGCTGCTGGGATTGGGCT 641 CTGGCCATCTGCAACCCTGAACGTGCAGGTTGGAGAACGAATGAATACAAATTTTATTGTAAAATAAAAATGTATATAAA 721 GCATATGAGCACACAAATGGACAATAAATGCCACAGCGCAAAAGCGTGTAGCGAGCCTGCCACGCTTGTGACTGGGGAGG 801 GGCTCCGTATAATCTCACCTTTGCAAGCGTTTATTTACTGATGTATGCGGCCACCACTGTGACCACCATCACTTACCGAT 881 TCACCAAAACTTAGGTAATATGGTCTTATTTTTGATTTAATTAAATATACGTGTAGCTCATGTATTTCACAGTTTAATAT 961 GAGAAGTGCTTTGGTCTTTATTTAGAAATTGGGTGATGTTTTTGTGCCCAGCAACATGCTGTAGGAATTTAACTCTTGTT 1041 TACATCCATTAGCCTCATGGTTTTGCTTCAAGTCTCAGCTTCCAAGAACCTATCGATGACTTACTGTGCATGGTCTGAGA 1121 CTAAGTAAAGGAAATTTCCTAAAGCTGTGAAATTTGAATTTTGCTTTAAATAAATGGAACTCACATGTGAAAAAAAAAAA 1201 AAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
|
||||||||||||||||||||
miRNA-target interactions (Predicted by miRanda) |
|
||||||||||||||||||||
DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
---|---|---|---|---|---|---|---|
miRNA:Target | ---- | ||||||
Validation Method |
|
||||||
Conditions | HEK293S | ||||||
Location of target site | 3'UTR | ||||||
Tools used in this research | TargetScan , miRTarCLIP , Piranha | ||||||
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM1084065. RNA binding protein: AGO2. Condition:CLIP_emetine_AbnovaAb
HITS-CLIP data was present in GSM1084079. RNA binding protein: AGO2. Condition:CLIP_hippuristanol_rep2_AbnovaAb
... - Karginov FV; Hannon GJ, 2013, Genes & development. |
||||||
miRNA-target interactions (Provided by authors) |
|
||||||
Article |
- Karginov FV; Hannon GJ - Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
|
CLIP-seq Support 1 for dataset GSM1084065 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_emetine_AbnovaAb |
Location of target site | ENST00000310903.5 | 3UTR | CCAAGGUGUGUGUGUCAG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset GSM1084079 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_hippuristanol_rep2_AbnovaAb |
Location of target site | ENST00000310903.5 | 3UTR | CCAAGGUGUGUGUGUCAG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
---|---|---|---|---|---|---|---|
|
MiRNA-Target Expression Profile (TCGA) | |||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
102 hsa-miR-3622b-3p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
![]() |
![]() |
|||||||
Strong evidence | Less strong evidence | |||||||||||
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
|||||
MIRT076713 | NUFIP2 | NUFIP2, FMR1 interacting protein 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT080212 | PRKACB | protein kinase cAMP-activated catalytic subunit beta | ![]() |
![]() |
2 | 2 | ||||||
MIRT081398 | GTPBP3 | GTP binding protein 3, mitochondrial | ![]() |
![]() |
2 | 2 | ||||||
MIRT107158 | ZBTB43 | zinc finger and BTB domain containing 43 | ![]() |
![]() |
2 | 2 | ||||||
MIRT254071 | BACH1 | BTB domain and CNC homolog 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT409790 | FOXO3 | forkhead box O3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT448679 | MAPK9 | mitogen-activated protein kinase 9 | ![]() |
![]() |
2 | 2 | ||||||
MIRT450206 | ABHD15 | abhydrolase domain containing 15 | ![]() |
![]() |
2 | 2 | ||||||
MIRT486621 | PDK3 | pyruvate dehydrogenase kinase 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT489267 | TTLL1 | tubulin tyrosine ligase like 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT493117 | MKNK2 | MAP kinase interacting serine/threonine kinase 2 | ![]() |
![]() |
2 | 4 | ||||||
MIRT494570 | BAK1 | BCL2 antagonist/killer 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT497395 | RALY | RALY heterogeneous nuclear ribonucleoprotein | ![]() |
![]() |
2 | 2 | ||||||
MIRT504900 | CCDC86 | coiled-coil domain containing 86 | ![]() |
![]() |
2 | 2 | ||||||
MIRT505192 | USP46 | ubiquitin specific peptidase 46 | ![]() |
![]() |
2 | 4 | ||||||
MIRT506522 | MSANTD4 | Myb/SANT DNA binding domain containing 4 with coiled-coils | ![]() |
![]() |
2 | 6 | ||||||
MIRT508162 | ABCC5 | ATP binding cassette subfamily C member 5 | ![]() |
![]() |
2 | 8 | ||||||
MIRT508545 | PARVG | parvin gamma | ![]() |
![]() |
2 | 4 | ||||||
MIRT509850 | BIRC5 | baculoviral IAP repeat containing 5 | ![]() |
![]() |
2 | 6 | ||||||
MIRT510088 | PPWD1 | peptidylprolyl isomerase domain and WD repeat containing 1 | ![]() |
![]() |
2 | 8 | ||||||
MIRT512094 | CRK | CRK proto-oncogene, adaptor protein | ![]() |
![]() |
2 | 4 | ||||||
MIRT515381 | RPL7 | ribosomal protein L7 | ![]() |
![]() |
2 | 2 | ||||||
MIRT519176 | SCO1 | SCO1, cytochrome c oxidase assembly protein | ![]() |
![]() |
2 | 2 | ||||||
MIRT519964 | ZCCHC8 | zinc finger CCHC-type containing 8 | ![]() |
![]() |
2 | 2 | ||||||
MIRT522292 | NKAP | NFKB activating protein | ![]() |
![]() |
2 | 2 | ||||||
MIRT523160 | HMGB2 | high mobility group box 2 | ![]() |
![]() |
2 | 4 | ||||||
MIRT525314 | FANCA | Fanconi anemia complementation group A | ![]() |
![]() |
2 | 2 | ||||||
MIRT528194 | PLEKHM2 | pleckstrin homology and RUN domain containing M2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT532885 | ZNF451 | zinc finger protein 451 | ![]() |
![]() |
2 | 2 | ||||||
MIRT538209 | CYR61 | cysteine rich angiogenic inducer 61 | ![]() |
![]() |
2 | 2 | ||||||
MIRT539497 | ACTN4 | actinin alpha 4 | ![]() |
![]() |
2 | 2 | ||||||
MIRT554600 | RRAGC | Ras related GTP binding C | ![]() |
![]() |
2 | 2 | ||||||
MIRT562444 | DCTN6 | dynactin subunit 6 | ![]() |
![]() |
2 | 2 | ||||||
MIRT562748 | AOC3 | amine oxidase, copper containing 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT565798 | SEC14L5 | SEC14 like lipid binding 5 | ![]() |
![]() |
2 | 2 | ||||||
MIRT565838 | SCML2 | Scm polycomb group protein like 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT566072 | RCC2 | regulator of chromosome condensation 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT566104 | RBPJ | recombination signal binding protein for immunoglobulin kappa J region | ![]() |
![]() |
2 | 2 | ||||||
MIRT572407 | MRPS14 | mitochondrial ribosomal protein S14 | ![]() |
![]() |
2 | 2 | ||||||
MIRT576198 | Vsig2 | V-set and immunoglobulin domain containing 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT576313 | Acbd7 | acyl-Coenzyme A binding domain containing 7 | ![]() |
![]() |
2 | 2 | ||||||
MIRT576651 | Mill2 | MHC I like leukocyte 2 | ![]() |
1 | 1 | |||||||
MIRT576859 | Socs6 | suppressor of cytokine signaling 6 | ![]() |
![]() |
2 | 2 | ||||||
MIRT606819 | BICD2 | BICD cargo adaptor 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT610738 | NUDT16 | nudix hydrolase 16 | ![]() |
![]() |
2 | 4 | ||||||
MIRT614798 | RORA | RAR related orphan receptor A | ![]() |
![]() |
2 | 2 | ||||||
MIRT619006 | NTMT1 | N-terminal Xaa-Pro-Lys N-methyltransferase 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT619420 | NOS1AP | nitric oxide synthase 1 adaptor protein | ![]() |
![]() |
2 | 2 | ||||||
MIRT620405 | MYO1H | myosin IH | ![]() |
![]() |
2 | 2 | ||||||
MIRT624869 | ABHD13 | abhydrolase domain containing 13 | ![]() |
![]() |
2 | 2 | ||||||
MIRT633527 | ZFP30 | ZFP30 zinc finger protein | ![]() |
![]() |
2 | 2 | ||||||
MIRT633927 | DNAH9 | dynein axonemal heavy chain 9 | ![]() |
![]() |
2 | 2 | ||||||
MIRT636611 | CLIC5 | chloride intracellular channel 5 | ![]() |
![]() |
2 | 2 | ||||||
MIRT640616 | MIOX | myo-inositol oxygenase | ![]() |
![]() |
2 | 2 | ||||||
MIRT642605 | C14orf180 | chromosome 14 open reading frame 180 | ![]() |
![]() |
2 | 2 | ||||||
MIRT644624 | SRSF2 | serine and arginine rich splicing factor 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT655113 | PHLDA3 | pleckstrin homology like domain family A member 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT658976 | DNAJB5 | DnaJ heat shock protein family (Hsp40) member B5 | ![]() |
![]() |
2 | 4 | ||||||
MIRT661495 | CHMP1B | charged multivesicular body protein 1B | ![]() |
![]() |
2 | 2 | ||||||
MIRT662996 | TMEM59 | transmembrane protein 59 | ![]() |
![]() |
2 | 2 | ||||||
MIRT663075 | SFR1 | SWI5 dependent homologous recombination repair protein 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT665405 | WEE1 | WEE1 G2 checkpoint kinase | ![]() |
![]() |
2 | 2 | ||||||
MIRT666106 | SSR1 | signal sequence receptor subunit 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT669562 | ALDOA | aldolase, fructose-bisphosphate A | ![]() |
![]() |
2 | 2 | ||||||
MIRT670070 | ZNF783 | zinc finger family member 783 | ![]() |
![]() |
2 | 2 | ||||||
MIRT671192 | ZNF891 | zinc finger protein 891 | ![]() |
![]() |
2 | 2 | ||||||
MIRT675361 | KLHL26 | kelch like family member 26 | ![]() |
![]() |
2 | 2 | ||||||
MIRT678857 | LINC00598 | long intergenic non-protein coding RNA 598 | ![]() |
![]() |
2 | 2 | ||||||
MIRT679443 | C19orf52 | translocase of inner mitochondrial membrane 29 | ![]() |
![]() |
2 | 2 | ||||||
MIRT684163 | ALDH1B1 | aldehyde dehydrogenase 1 family member B1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT684543 | ZNF460 | zinc finger protein 460 | ![]() |
![]() |
2 | 2 | ||||||
MIRT684674 | SLC2A11 | solute carrier family 2 member 11 | ![]() |
![]() |
2 | 2 | ||||||
MIRT684971 | MINOS1 | mitochondrial inner membrane organizing system 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT686006 | NEK4 | NIMA related kinase 4 | ![]() |
![]() |
2 | 2 | ||||||
MIRT687758 | KIAA1328 | KIAA1328 | ![]() |
![]() |
2 | 2 | ||||||
MIRT688961 | ATXN3 | ataxin 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT689409 | UQCR11 | ubiquinol-cytochrome c reductase, complex III subunit XI | ![]() |
![]() |
2 | 2 | ||||||
MIRT690001 | MMP17 | matrix metallopeptidase 17 | ![]() |
![]() |
2 | 2 | ||||||
MIRT690165 | ELP3 | elongator acetyltransferase complex subunit 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT690202 | C5orf45 | MRN complex interacting protein | ![]() |
![]() |
2 | 2 | ||||||
MIRT690477 | ZNF33A | zinc finger protein 33A | ![]() |
![]() |
2 | 2 | ||||||
MIRT690566 | MICA | MHC class I polypeptide-related sequence A | ![]() |
![]() |
2 | 2 | ||||||
MIRT692160 | C10orf111 | chromosome 10 open reading frame 111 | ![]() |
![]() |
2 | 2 | ||||||
MIRT693430 | PLGLB2 | plasminogen-like B2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT693547 | ZNF708 | zinc finger protein 708 | ![]() |
![]() |
2 | 2 | ||||||
MIRT693697 | PLGLB1 | plasminogen-like B1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT695006 | HSPA6 | heat shock protein family A (Hsp70) member 6 | ![]() |
![]() |
2 | 2 | ||||||
MIRT698525 | TFRC | transferrin receptor | ![]() |
![]() |
2 | 2 | ||||||
MIRT699574 | SIKE1 | suppressor of IKBKE 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT703446 | FYTTD1 | forty-two-three domain containing 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT704397 | CTSS | cathepsin S | ![]() |
![]() |
2 | 2 | ||||||
MIRT704485 | CPT1A | carnitine palmitoyltransferase 1A | ![]() |
![]() |
2 | 2 | ||||||
MIRT709822 | STPG1 | sperm tail PG-rich repeat containing 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT710859 | COQ7 | coenzyme Q7, hydroxylase | ![]() |
![]() |
2 | 2 | ||||||
MIRT711893 | INSIG2 | insulin induced gene 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT713796 | CPLX2 | complexin 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT718800 | C1GALT1C1 | C1GALT1 specific chaperone 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT719501 | SEC24B | SEC24 homolog B, COPII coat complex component | ![]() |
![]() |
2 | 2 | ||||||
MIRT719722 | PDE6B | phosphodiesterase 6B | ![]() |
![]() |
2 | 2 | ||||||
MIRT722204 | URM1 | ubiquitin related modifier 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT723523 | CLPTM1L | CLPTM1 like | ![]() |
![]() |
2 | 2 | ||||||
MIRT725471 | GRAP2 | GRB2-related adaptor protein 2 | ![]() |
![]() |
2 | 2 |
miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|