pre-miRNA Information
pre-miRNA hsa-mir-4524a   
Genomic Coordinates chr17: 69099564 - 69099632
Description Homo sapiens miR-4524a stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4524a-5p
Sequence 6| AUAGCAGCAUGAACCUGUCUCA |27
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1322445998 8 dbSNP
rs1350790266 13 dbSNP
rs1263495678 21 dbSNP
Putative Targets

Gene Information
Gene Symbol ASAH2   
Synonyms BCDase, HNAC1, LCDase, N-CDase, NCDase
Description N-acylsphingosine amidohydrolase 2
Transcript NM_001143974   
Other Transcripts NM_019893   
Expression
Putative miRNA Targets on ASAH2
3'UTR of ASAH2
(miRNA target sites are highlighted)
>ASAH2|NM_001143974|3'UTR
   1 TGAAAAGTTGATAGATCATTTAAAGAACAGCTTTACTCTCTACACATTATATAAGTGATTTCAAATGAATGTGAAAAAAA
  81 AAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' acUC-UGUCCAAGUACGACGAUa 5'
            || |||| ||:|  || ||| 
Target 5' aaAGAACAGCTTTACTCT-CTAc 3'
22 - 43 88.00 -5.60
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN29410838 152 COSMIC
COSN28475638 188 COSMIC
COSN28714656 199 COSMIC
COSN29698299 228 COSMIC
COSN20914875 240 COSMIC
COSN28811868 250 COSMIC
COSN20555490 748 COSMIC
COSN28546587 1355 COSMIC
COSN6478816 1491 COSMIC
COSN20294793 1762 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1224876343 1 dbSNP
rs1426361274 15 dbSNP
rs1269049927 31 dbSNP
rs1260479231 41 dbSNP
rs574856071 49 dbSNP
rs1166677154 67 dbSNP
rs1467814095 71 dbSNP
rs1214112928 72 dbSNP
rs1269281172 83 dbSNP
rs1453509922 89 dbSNP
rs1191363444 91 dbSNP
rs1241542253 92 dbSNP
rs1478205218 97 dbSNP
rs1403201682 107 dbSNP
rs1416350860 111 dbSNP
rs1384265069 117 dbSNP
rs1164198997 119 dbSNP
rs1393384476 122 dbSNP
rs1380153419 126 dbSNP
rs1317231755 137 dbSNP
rs1326463855 138 dbSNP
rs1401313577 139 dbSNP
rs1158701940 145 dbSNP
rs1441064183 147 dbSNP
rs1377953361 148 dbSNP
rs1175933554 149 dbSNP
rs1017014297 150 dbSNP
rs1480605990 150 dbSNP
rs1321850114 151 dbSNP
rs1382687125 151 dbSNP
rs1459132544 152 dbSNP
rs1266964629 153 dbSNP
rs1348306240 153 dbSNP
rs1203573258 154 dbSNP
rs1247617154 154 dbSNP
rs1383304324 156 dbSNP
rs1224295365 157 dbSNP
rs980222554 157 dbSNP
rs1195914857 159 dbSNP
rs1450062724 159 dbSNP
rs1291394782 161 dbSNP
rs1475151289 168 dbSNP
rs1370518546 175 dbSNP
rs1452125966 176 dbSNP
rs1157563464 177 dbSNP
rs1360783574 179 dbSNP
rs1240963639 180 dbSNP
rs1355855843 181 dbSNP
rs1307190566 182 dbSNP
rs1415270297 183 dbSNP
rs1223092439 184 dbSNP
rs1368249859 185 dbSNP
rs1295036788 186 dbSNP
rs1434090144 187 dbSNP
rs1312410218 188 dbSNP
rs1298502848 189 dbSNP
rs868803905 190 dbSNP
rs1385505648 191 dbSNP
rs9416380 192 dbSNP
rs373974597 193 dbSNP
rs1426548454 194 dbSNP
rs372193823 195 dbSNP
rs1171385681 197 dbSNP
rs1242525520 197 dbSNP
rs1304454677 197 dbSNP
rs1309669961 197 dbSNP
rs1369899094 197 dbSNP
rs1370881909 197 dbSNP
rs1374280097 197 dbSNP
rs1378903150 197 dbSNP
rs1381392479 197 dbSNP
rs1394227635 197 dbSNP
rs1441133093 197 dbSNP
rs1466107687 197 dbSNP
rs867389598 197 dbSNP
rs1479702236 198 dbSNP
rs1259270165 199 dbSNP
rs1290732306 199 dbSNP
rs1324066493 199 dbSNP
rs1331988589 199 dbSNP
rs1367155948 199 dbSNP
rs1387630302 199 dbSNP
rs1402153741 199 dbSNP
rs1438453006 199 dbSNP
rs1192615168 201 dbSNP
rs1212191728 201 dbSNP
rs1252833043 201 dbSNP
rs1259000607 201 dbSNP
rs1488583476 201 dbSNP
rs1427443349 202 dbSNP
rs1163944252 203 dbSNP
rs1171223343 203 dbSNP
rs1350296467 203 dbSNP
rs1425711486 203 dbSNP
rs1437393409 203 dbSNP
rs1459864532 203 dbSNP
rs1161130821 205 dbSNP
rs1232038957 205 dbSNP
rs1278976503 205 dbSNP
rs1295535170 205 dbSNP
rs1359874177 205 dbSNP
rs1399492403 205 dbSNP
rs1268627763 207 dbSNP
rs1217541875 211 dbSNP
rs1289584386 212 dbSNP
rs1489405136 216 dbSNP
rs1258995410 217 dbSNP
rs1223264840 223 dbSNP
rs1258591138 224 dbSNP
rs1215202021 226 dbSNP
rs1485229048 227 dbSNP
rs1193738957 230 dbSNP
rs1187198117 231 dbSNP
rs1264790124 231 dbSNP
rs1414198039 231 dbSNP
rs1474168007 231 dbSNP
rs150599615 231 dbSNP
rs1237998125 232 dbSNP
rs1287516759 232 dbSNP
rs1484421341 232 dbSNP
rs201330700 232 dbSNP
rs763380647 232 dbSNP
rs1312678358 233 dbSNP
rs1303393089 237 dbSNP
rs1407024264 238 dbSNP
rs1047279734 239 dbSNP
rs539716343 240 dbSNP
rs1344573131 243 dbSNP
rs1362498580 249 dbSNP
rs61856759 250 dbSNP
rs1334022657 251 dbSNP
rs1360242529 262 dbSNP
rs1450902602 263 dbSNP
rs1296780667 267 dbSNP
rs1401419672 277 dbSNP
rs61856758 284 dbSNP
rs1311003229 291 dbSNP
rs1345043738 297 dbSNP
rs1218114967 300 dbSNP
rs970231370 308 dbSNP
rs1287762064 311 dbSNP
rs1444031771 312 dbSNP
rs1466158427 337 dbSNP
rs1360250254 339 dbSNP
rs1178791544 341 dbSNP
rs1458069275 346 dbSNP
rs1024951111 360 dbSNP
rs1014530080 362 dbSNP
rs1477196240 372 dbSNP
rs896102988 373 dbSNP
rs1426015266 376 dbSNP
rs1416637441 391 dbSNP
rs867624310 405 dbSNP
rs1447997063 407 dbSNP
rs1333854390 408 dbSNP
rs1368325084 410 dbSNP
rs1435361337 417 dbSNP
rs1274901626 418 dbSNP
rs1346533067 423 dbSNP
rs1212692698 431 dbSNP
rs1257354894 433 dbSNP
rs1318261403 442 dbSNP
rs1200292315 454 dbSNP
rs1250979954 463 dbSNP
rs1450056134 465 dbSNP
rs1198488532 470 dbSNP
rs1247319287 477 dbSNP
rs1479067408 485 dbSNP
rs1169088860 493 dbSNP
rs1194883666 495 dbSNP
rs1466660547 502 dbSNP
rs1173229375 506 dbSNP
rs201798837 509 dbSNP
rs1404341815 517 dbSNP
rs1323841902 519 dbSNP
rs1332252374 537 dbSNP
rs1428057261 538 dbSNP
rs1341049614 540 dbSNP
rs1220437499 546 dbSNP
rs1271019254 547 dbSNP
rs1355411200 554 dbSNP
rs1209428412 572 dbSNP
rs1290868858 585 dbSNP
rs1259971138 589 dbSNP
rs1191588190 605 dbSNP
rs1262612371 607 dbSNP
rs1478421949 615 dbSNP
rs1030672236 617 dbSNP
rs1367044554 620 dbSNP
rs1185989349 630 dbSNP
rs1163421849 637 dbSNP
rs1392417366 638 dbSNP
rs1417552900 650 dbSNP
rs1297006954 651 dbSNP
rs1401718071 652 dbSNP
rs1291400947 653 dbSNP
rs999223376 667 dbSNP
rs1245225393 675 dbSNP
rs1483658066 677 dbSNP
rs903601643 683 dbSNP
rs1257836930 689 dbSNP
rs1488676132 690 dbSNP
rs1219343810 700 dbSNP
rs1254700481 701 dbSNP
rs1423805660 703 dbSNP
rs1043517324 707 dbSNP
rs1370604044 708 dbSNP
rs1437410916 712 dbSNP
rs1157704664 716 dbSNP
rs1380338399 717 dbSNP
rs1420995539 718 dbSNP
rs1357302590 719 dbSNP
rs1322774493 729 dbSNP
rs1258434169 737 dbSNP
rs1220292534 739 dbSNP
rs1355756 748 dbSNP
rs1294492421 749 dbSNP
rs1354429104 751 dbSNP
rs1242392042 759 dbSNP
rs1358875274 764 dbSNP
rs1254409294 768 dbSNP
rs1483191707 770 dbSNP
rs1201579636 775 dbSNP
rs1258649463 780 dbSNP
rs1396259226 787 dbSNP
rs1238423825 802 dbSNP
rs942496096 809 dbSNP
rs1173299841 811 dbSNP
rs1376621050 828 dbSNP
rs1380628827 833 dbSNP
rs1174277504 845 dbSNP
rs1397765992 855 dbSNP
rs1300588469 858 dbSNP
rs571103917 859 dbSNP
rs879123446 860 dbSNP
rs1171802858 864 dbSNP
rs1234557465 871 dbSNP
rs1467565066 895 dbSNP
rs1316389274 910 dbSNP
rs1198399582 938 dbSNP
rs1259153335 942 dbSNP
rs1458797335 951 dbSNP
rs1189116927 955 dbSNP
rs1266008101 958 dbSNP
rs1433271337 959 dbSNP
rs1200838312 963 dbSNP
rs1421372777 972 dbSNP
rs1465101874 977 dbSNP
rs1171089063 996 dbSNP
rs1402256449 1008 dbSNP
rs1463678075 1016 dbSNP
rs1292038469 1017 dbSNP
rs1366922700 1029 dbSNP
rs1371552553 1037 dbSNP
rs1285343151 1038 dbSNP
rs1339045426 1040 dbSNP
rs1218613881 1043 dbSNP
rs1259049578 1044 dbSNP
rs1309661476 1049 dbSNP
rs1218838520 1051 dbSNP
rs1169925797 1066 dbSNP
rs1424639177 1067 dbSNP
rs1211102194 1073 dbSNP
rs1260464877 1089 dbSNP
rs1477443325 1095 dbSNP
rs1425454465 1100 dbSNP
rs1421841818 1112 dbSNP
rs1162879736 1118 dbSNP
rs1358502649 1125 dbSNP
rs1458666856 1126 dbSNP
rs1258302434 1132 dbSNP
rs1358722918 1136 dbSNP
rs1181868939 1150 dbSNP
rs1279390839 1152 dbSNP
rs1364453659 1163 dbSNP
rs1226950032 1166 dbSNP
rs1312543086 1172 dbSNP
rs1247782147 1190 dbSNP
rs1460800935 1192 dbSNP
rs1487694359 1193 dbSNP
rs1217168051 1195 dbSNP
rs1265268942 1203 dbSNP
rs1482270779 1204 dbSNP
rs1181068361 1210 dbSNP
rs1238782659 1211 dbSNP
rs764166473 1214 dbSNP
rs1424562933 1225 dbSNP
rs1165732837 1226 dbSNP
rs1378482375 1228 dbSNP
rs1419187267 1231 dbSNP
rs1298943631 1235 dbSNP
rs1337129664 1237 dbSNP
rs1446511091 1240 dbSNP
rs1302614594 1241 dbSNP
rs1380382039 1245 dbSNP
rs1247666303 1246 dbSNP
rs1306636012 1248 dbSNP
rs1352267384 1257 dbSNP
rs1240231282 1261 dbSNP
rs1310339408 1265 dbSNP
rs1209359956 1273 dbSNP
rs1250567211 1274 dbSNP
rs1237795453 1290 dbSNP
rs1462487878 1294 dbSNP
rs1185587753 1297 dbSNP
rs1236565728 1300 dbSNP
rs1439387955 1304 dbSNP
rs1318061912 1311 dbSNP
rs1410322624 1317 dbSNP
rs1413217169 1318 dbSNP
rs1176015290 1343 dbSNP
rs1360043965 1345 dbSNP
rs1454932899 1349 dbSNP
rs1329810037 1351 dbSNP
rs1244999095 1355 dbSNP
rs1447118988 1359 dbSNP
rs1280735063 1361 dbSNP
rs1323174216 1363 dbSNP
rs1312237990 1366 dbSNP
rs878957940 1367 dbSNP
rs889640076 1379 dbSNP
rs1218501773 1384 dbSNP
rs1279257059 1387 dbSNP
rs1325653191 1403 dbSNP
rs1196521493 1408 dbSNP
rs1257232769 1416 dbSNP
rs1457465858 1416 dbSNP
rs1182829841 1429 dbSNP
rs1451339576 1430 dbSNP
rs1234631602 1432 dbSNP
rs1382677050 1439 dbSNP
rs1477085446 1439 dbSNP
rs1426471721 1443 dbSNP
rs1513247 1452 dbSNP
rs4641410 1462 dbSNP
rs1394713402 1466 dbSNP
rs1297996333 1473 dbSNP
rs1330474852 1479 dbSNP
rs1328788919 1484 dbSNP
rs1051428979 1487 dbSNP
rs4245004 1491 dbSNP
rs1347289597 1492 dbSNP
rs1216773028 1504 dbSNP
rs1277280171 1514 dbSNP
rs1318373909 1517 dbSNP
rs1196536926 1518 dbSNP
rs1275402845 1520 dbSNP
rs1450149849 1531 dbSNP
rs1220925930 1532 dbSNP
rs1269748734 1537 dbSNP
rs1481695283 1544 dbSNP
rs1370470614 1545 dbSNP
rs1475952538 1546 dbSNP
rs1161734889 1548 dbSNP
rs1388126618 1552 dbSNP
rs1404436993 1559 dbSNP
rs1323619323 1565 dbSNP
rs1366921468 1568 dbSNP
rs1297512121 1569 dbSNP
rs1398029209 1569 dbSNP
rs1340836915 1575 dbSNP
rs1444160721 1588 dbSNP
rs1229324655 1601 dbSNP
rs1381011127 1603 dbSNP
rs1228015531 1606 dbSNP
rs547710722 1608 dbSNP
rs200833764 1609 dbSNP
rs1215007711 1614 dbSNP
rs1263082607 1618 dbSNP
rs1448141658 1621 dbSNP
rs1195738486 1627 dbSNP
rs530058292 1637 dbSNP
rs1367136582 1643 dbSNP
rs1472231702 1662 dbSNP
rs1335049347 1663 dbSNP
rs2813181 1665 dbSNP
rs1417337163 1667 dbSNP
rs568505887 1669 dbSNP
rs796311126 1670 dbSNP
rs1385577893 1675 dbSNP
rs1383896838 1680 dbSNP
rs1331930387 1685 dbSNP
rs1339683391 1700 dbSNP
rs1447483661 1708 dbSNP
rs1316006300 1714 dbSNP
rs1361931720 1719 dbSNP
rs1238047862 1720 dbSNP
rs1285450310 1729 dbSNP
rs1210251189 1734 dbSNP
rs1207433296 1740 dbSNP
rs548652527 1746 dbSNP
rs1489243440 1753 dbSNP
rs2813182 1762 dbSNP
rs1183761465 1767 dbSNP
rs1244586337 1769 dbSNP
rs1437510814 1778 dbSNP
rs1157499470 1790 dbSNP
rs1363929265 1800 dbSNP
rs1455243355 1811 dbSNP
rs1170782021 1813 dbSNP
rs1401157469 1820 dbSNP
rs375515526 1839 dbSNP
rs1338533354 1858 dbSNP
rs1385220354 1892 dbSNP
rs1444902886 1924 dbSNP
rs797014303 1926 dbSNP
rs1310830387 1929 dbSNP
rs1241721385 1936 dbSNP
rs1254779569 1937 dbSNP
rs1372490618 1956 dbSNP
rs531744023 1957 dbSNP
rs1334554433 1967 dbSNP
rs1177654104 1975 dbSNP
rs1250571855 1980 dbSNP
rs1420364003 1988 dbSNP
rs1196197300 1999 dbSNP
rs1306710415 2003 dbSNP
rs1376992689 2004 dbSNP
rs1387768791 2006 dbSNP
rs1178294899 2009 dbSNP
rs1397873904 2012 dbSNP
rs562835961 2016 dbSNP
rs2813183 2017 dbSNP
rs1397232650 2019 dbSNP
rs1301457206 2021 dbSNP
rs1299372343 2028 dbSNP
rs532330844 2031 dbSNP
rs77165194 2035 dbSNP
rs560379211 2038 dbSNP
rs1160571280 2052 dbSNP
rs1197549458 2073 dbSNP
rs1251509341 2074 dbSNP
rs1468511538 2090 dbSNP
rs1207518647 2092 dbSNP
rs1266110122 2116 dbSNP
rs1480693192 2117 dbSNP
rs1201540348 2130 dbSNP
rs1421119340 2133 dbSNP
rs1477380463 2134 dbSNP
rs1170958287 2137 dbSNP
rs878945379 2141 dbSNP
rs879030936 2159 dbSNP
rs933931207 2163 dbSNP
rs1365064037 2166 dbSNP
rs1406051823 2167 dbSNP
rs1305497535 2173 dbSNP
rs1339163779 2182 dbSNP
rs1266515806 2189 dbSNP
rs878888202 2191 dbSNP
rs1308869061 2193 dbSNP
rs1488426181 2194 dbSNP
rs1290343896 2205 dbSNP
rs1490371551 2212 dbSNP
rs1224554599 2217 dbSNP
rs1260920156 2234 dbSNP
rs1487960798 2236 dbSNP
rs918532768 2237 dbSNP
rs972706510 2240 dbSNP
rs1430474877 2243 dbSNP
rs941389618 2252 dbSNP
rs1349144326 2256 dbSNP
rs1384616957 2256 dbSNP
rs1425400990 2260 dbSNP
rs1165618705 2271 dbSNP
rs1359149262 2273 dbSNP
rs1279553627 2280 dbSNP
rs1364559871 2282 dbSNP
rs1393864276 2299 dbSNP
rs1313941632 2304 dbSNP
rs1359784194 2323 dbSNP
rs1247527131 2324 dbSNP
rs1283312486 2325 dbSNP
rs1316249848 2327 dbSNP
rs1234606085 2333 dbSNP
rs1482158298 2335 dbSNP
rs1180282271 2341 dbSNP
rs1347707923 2344 dbSNP
rs909958387 2346 dbSNP
rs1185468806 2355 dbSNP
rs1378567929 2362 dbSNP
rs879165092 2365 dbSNP
rs1281792932 2366 dbSNP
rs2942774 2368 dbSNP
rs1160473826 2369 dbSNP
rs1347258420 2370 dbSNP
rs980170331 2377 dbSNP
rs1336561737 2383 dbSNP
rs1454293098 2401 dbSNP
rs970610427 2410 dbSNP
rs1372024449 2425 dbSNP
rs1024482710 2431 dbSNP
rs993451757 2446 dbSNP
rs1310434942 2449 dbSNP
rs1202758912 2452 dbSNP
rs1263579614 2455 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084064. RNA binding protein: AGO2. Condition:CLIP_noemetine_AbnovaAb HITS-CLIP data was present in GSM1084065. RNA binding protein: AGO2. Condition:CLIP_emetine_AbnovaAb HITS-CLIP data was present in GSM1084068. RNA binding protein: AGO2. Condition:CLIP_noemetine_SigmaAb HITS-CLIP data was present in GSM1084072. RNA binding protein: AGO2. Condition:CLIP_nohippuristanol_rep1_AbnovaAb HITS-CLIP data was present in GSM1084073. RNA binding protein: AGO2. Condition:CLIP_hippuristanol_rep1_AbnovaAb HITS-CLIP data was present in GSM1084075. RNA binding protein: AGO2. Condition:CLIP_hippuristanol_rep1_SantaCruzAb HITS-CLIP data was present in GSM1084079. RNA binding protein: AGO2. Condition:CLIP_hippuristanol_rep2_AbnovaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084064
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_noemetine_AbnovaAb
Location of target site ENST00000395526.4 | 3UTR | AUGGGGUGGAGGGGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGUU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM1084065
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_emetine_AbnovaAb
Location of target site ENST00000395526.4 | 3UTR | UAAUGGGGUGGAGGGGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGUUU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM1084068
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_noemetine_SigmaAb
Location of target site ENST00000395526.4 | 3UTR | AAUGGGGUGGAGGGGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGAGAGAGAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM1084072
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_nohippuristanol_rep1_AbnovaAb
Location of target site ENST00000395526.4 | 3UTR | AAUGGGGUGGAGGGGUGUGUGUGUGUGUGUGUGUGUGUGUGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 5 for dataset GSM1084073
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_hippuristanol_rep1_AbnovaAb
Location of target site ENST00000395526.4 | 3UTR | UAAUGGGGUGGAGGGGUGUGUGUGUGUGUGUGUGUGUGUGUGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 6 for dataset GSM1084075
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_hippuristanol_rep1_SantaCruzAb
Location of target site ENST00000395526.4 | 3UTR | AAUGGGGUGGAGGGGUGUGUGUGUGUGUGUGUGUGUGUGUGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 7 for dataset GSM1084079
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_hippuristanol_rep2_AbnovaAb
Location of target site ENST00000395526.4 | 3UTR | AAUGGGGUGGAGGGGUGUGUGUGUGUGUGUGUGUGUGUGUGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
180 hsa-miR-4524a-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT055251 CNNM2 cyclin and CBS domain divalent metal cation transport mediator 2 2 2
MIRT055826 PLEKHA1 pleckstrin homology domain containing A1 2 2
MIRT060573 CCND1 cyclin D1 2 2
MIRT061013 C1ORF21 chromosome 1 open reading frame 21 2 6
MIRT064694 CCND2 cyclin D2 2 4
MIRT075268 SNTB2 syntrophin beta 2 2 4
MIRT079668 NAPG NSF attachment protein gamma 2 12
MIRT081651 CCNE1 cyclin E1 2 4
MIRT082996 PNPLA6 patatin like phospholipase domain containing 6 2 2
MIRT083463 RALGAPB Ral GTPase activating protein non-catalytic beta subunit 2 4
MIRT085755 RIF1 replication timing regulatory factor 1 2 2
MIRT086022 UBR3 ubiquitin protein ligase E3 component n-recognin 3 (putative) 2 2
MIRT087431 ZNRF3 zinc and ring finger 3 2 2
MIRT088786 SOCS5 suppressor of cytokine signaling 5 2 2
MIRT089221 ACTR2 ARP2 actin related protein 2 homolog 2 2
MIRT093696 PI4K2B phosphatidylinositol 4-kinase type 2 beta 2 6
MIRT095090 SEC24A SEC24 homolog A, COPII coat complex component 2 4
MIRT096249 CANX calnexin 2 2
MIRT100215 PPP1R11 protein phosphatase 1 regulatory inhibitor subunit 11 2 2
MIRT100746 VEGFA vascular endothelial growth factor A 2 12
MIRT100904 CD2AP CD2 associated protein 2 2
MIRT102647 UBN2 ubinuclein 2 2 10
MIRT103882 FOXK1 forkhead box K1 2 2
MIRT104246 DMTF1 cyclin D binding myb like transcription factor 1 2 2
MIRT106310 ZFHX4 zinc finger homeobox 4 2 6
MIRT107696 RECK reversion inducing cysteine rich protein with kazal motifs 2 2
MIRT114943 CHAC1 ChaC glutathione specific gamma-glutamylcyclotransferase 1 2 2
MIRT117671 SCAMP4 secretory carrier membrane protein 4 2 2
MIRT133799 SKI SKI proto-oncogene 2 2
MIRT140167 SPRED1 sprouty related EVH1 domain containing 1 2 2
MIRT142279 DCTN5 dynactin subunit 5 2 8
MIRT143288 N4BP1 NEDD4 binding protein 1 2 2
MIRT165939 CREBRF CREB3 regulatory factor 2 2
MIRT175251 PSAT1 phosphoserine aminotransferase 1 2 6
MIRT186381 PNRC2 proline rich nuclear receptor coactivator 2 2 2
MIRT191470 PPM1A protein phosphatase, Mg2+/Mn2+ dependent 1A 2 2
MIRT196480 TAOK1 TAO kinase 1 2 2
MIRT201470 SNRPB2 small nuclear ribonucleoprotein polypeptide B2 2 8
MIRT204615 HSPE1-MOB4 HSPE1-MOB4 readthrough 2 8
MIRT204646 MOB4 MOB family member 4, phocein 2 8
MIRT204749 BZW1 basic leucine zipper and W2 domains 1 2 12
MIRT206031 NUP50 nucleoporin 50 2 6
MIRT211196 FGF2 fibroblast growth factor 2 2 4
MIRT229353 ZNF449 zinc finger protein 449 2 2
MIRT247138 WEE1 WEE1 G2 checkpoint kinase 2 4
MIRT249461 ZNF691 zinc finger protein 691 2 4
MIRT256314 CDC42SE2 CDC42 small effector 2 2 2
MIRT258419 WIPI2 WD repeat domain, phosphoinositide interacting 2 2 2
MIRT265083 CHEK1 checkpoint kinase 1 2 2
MIRT270561 SETD1B SET domain containing 1B 2 2
MIRT274749 RAB3IP RAB3A interacting protein 2 2
MIRT277515 PPP2R5C protein phosphatase 2 regulatory subunit B'gamma 2 4
MIRT289642 CBX2 chromobox 2 2 2
MIRT301001 MTMR3 myotubularin related protein 3 2 2
MIRT307149 CTDSPL CTD small phosphatase like 2 4
MIRT309021 USP53 ubiquitin specific peptidase 53 2 2
MIRT314100 PIK3R1 phosphoinositide-3-kinase regulatory subunit 1 2 8
MIRT319338 CAPZA2 capping actin protein of muscle Z-line alpha subunit 2 2 2
MIRT320619 ZNRF2 zinc and ring finger 2 2 2
MIRT324285 LURAP1L leucine rich adaptor protein 1 like 2 2
MIRT446498 ASCC1 activating signal cointegrator 1 complex subunit 1 2 2
MIRT448437 TLL1 tolloid like 1 2 2
MIRT461537 ACTR3B ARP3 actin related protein 3 homolog B 2 2
MIRT463162 ZNF367 zinc finger protein 367 2 10
MIRT463493 ZC3H10 zinc finger CCCH-type containing 10 2 2
MIRT465154 TSC22D2 TSC22 domain family member 2 2 2
MIRT466418 TFAP2A transcription factor AP-2 alpha 2 8
MIRT468278 SFT2D2 SFT2 domain containing 2 2 2
MIRT469399 REL REL proto-oncogene, NF-kB subunit 2 6
MIRT471941 NR6A1 nuclear receptor subfamily 6 group A member 1 2 2
MIRT473688 MAPK8 mitogen-activated protein kinase 8 2 4
MIRT479618 CDC25A cell division cycle 25A 2 2
MIRT482098 AKT3 AKT serine/threonine kinase 3 2 4
MIRT483995 ATAD5 ATPase family, AAA domain containing 5 2 12
MIRT485205 PRKAR2A protein kinase cAMP-dependent type II regulatory subunit alpha 2 8
MIRT498763 C3orf38 chromosome 3 open reading frame 38 2 8
MIRT498961 ORC4 origin recognition complex subunit 4 2 8
MIRT499440 ODF2L outer dense fiber of sperm tails 2 like 2 8
MIRT500080 L2HGDH L-2-hydroxyglutarate dehydrogenase 2 8
MIRT500305 ZNF622 zinc finger protein 622 2 8
MIRT500410 ZMAT3 zinc finger matrin-type 3 2 8
MIRT500789 TLK1 tousled like kinase 1 2 6
MIRT500930 SRPR SRP receptor alpha subunit 2 6
MIRT500943 SREK1 splicing regulatory glutamic acid and lysine rich protein 1 2 8
MIRT501068 SMAD7 SMAD family member 7 2 8
MIRT501711 PARD6B par-6 family cell polarity regulator beta 2 2
MIRT502627 DDX3X DEAD-box helicase 3, X-linked 2 8
MIRT502910 CDCA4 cell division cycle associated 4 2 8
MIRT502935 CDC37L1 cell division cycle 37 like 1 2 8
MIRT504531 ZNF620 zinc finger protein 620 2 6
MIRT505106 YTHDC1 YTH domain containing 1 2 6
MIRT505337 TMEM245 transmembrane protein 245 2 6
MIRT505383 TMEM100 transmembrane protein 100 2 2
MIRT505678 SESTD1 SEC14 and spectrin domain containing 1 2 6
MIRT506157 PLAG1 PLAG1 zinc finger 2 8
MIRT506183 PHKA1 phosphorylase kinase regulatory subunit alpha 1 2 6
MIRT506475 MYO5A myosin VA 2 6
MIRT506826 KIF23 kinesin family member 23 2 6
MIRT507160 GAS2L3 growth arrest specific 2 like 3 2 2
MIRT507511 DYNLL2 dynein light chain LC8-type 2 2 4
MIRT507845 CCNE2 cyclin E2 2 6
MIRT510403 ZNF507 zinc finger protein 507 2 2
MIRT518078 TRIM35 tripartite motif containing 35 2 2
MIRT518982 NNT nicotinamide nucleotide transhydrogenase 2 4
MIRT521045 SLC2A3 solute carrier family 2 member 3 2 4
MIRT521190 SBNO1 strawberry notch homolog 1 2 6
MIRT522088 NUFIP2 NUFIP2, FMR1 interacting protein 2 2 4
MIRT524846 ARPP19 cAMP regulated phosphoprotein 19 2 2
MIRT527787 TMEM44 transmembrane protein 44 2 4
MIRT537803 EFNB2 ephrin B2 2 4
MIRT540830 GNAT1 G protein subunit alpha transducin 1 2 4
MIRT541140 PISD phosphatidylserine decarboxylase 2 2
MIRT541419 CBX4 chromobox 4 2 2
MIRT543517 PRSS21 protease, serine 21 2 2
MIRT543824 GSG1 germ cell associated 1 2 2
MIRT544959 UGT2B4 UDP glucuronosyltransferase family 2 member B4 2 2
MIRT545179 MAP4K2 mitogen-activated protein kinase kinase kinase kinase 2 2 4
MIRT545335 CCDC83 coiled-coil domain containing 83 2 2
MIRT545518 RSL24D1 ribosomal L24 domain containing 1 2 2
MIRT545670 DECR1 2,4-dienoyl-CoA reductase 1 2 2
MIRT545931 ZBTB44 zinc finger and BTB domain containing 44 2 4
MIRT546102 USP48 ubiquitin specific peptidase 48 2 4
MIRT546598 SALL1 spalt like transcription factor 1 2 4
MIRT546626 RTN4 reticulon 4 2 2
MIRT547651 KPNA3 karyopherin subunit alpha 3 2 2
MIRT547987 HCFC2 host cell factor C2 2 4
MIRT548717 CRK CRK proto-oncogene, adaptor protein 2 2
MIRT548931 CDK17 cyclin dependent kinase 17 2 2
MIRT549067 CACUL1 CDK2 associated cullin domain 1 2 2
MIRT549266 ASH1L ASH1 like histone lysine methyltransferase 2 2
MIRT550460 OSCAR osteoclast associated, immunoglobulin-like receptor 2 4
MIRT550806 FAM229B family with sequence similarity 229 member B 2 2
MIRT552024 DNAJC10 DnaJ heat shock protein family (Hsp40) member C10 2 2
MIRT552334 ZNF704 zinc finger protein 704 2 2
MIRT552732 YRDC yrdC N6-threonylcarbamoyltransferase domain containing 2 2
MIRT553795 SZRD1 SUZ RNA binding domain containing 1 2 4
MIRT554694 RNF149 ring finger protein 149 2 2
MIRT555133 PTPRD protein tyrosine phosphatase, receptor type D 2 2
MIRT555264 PRDM4 PR/SET domain 4 2 2
MIRT556848 KANK1 KN motif and ankyrin repeat domains 1 2 4
MIRT557474 GPR27 G protein-coupled receptor 27 2 4
MIRT558018 EXT1 exostosin glycosyltransferase 1 2 2
MIRT558498 CYP26B1 cytochrome P450 family 26 subfamily B member 1 2 4
MIRT558579 CREBL2 cAMP responsive element binding protein like 2 2 4
MIRT558610 COX6B1 cytochrome c oxidase subunit 6B1 2 4
MIRT558650 CNKSR3 CNKSR family member 3 2 2
MIRT558986 CA8 carbonic anhydrase 8 2 2
MIRT559141 BTN3A3 butyrophilin subfamily 3 member A3 2 2
MIRT559327 ATP5G3 ATP synthase, H+ transporting, mitochondrial Fo complex subunit C3 (subunit 9) 2 2
MIRT562021 LANCL1 LanC like 1 2 2
MIRT562869 KIAA1456 KIAA1456 2 2
MIRT563074 SLC25A12 solute carrier family 25 member 12 2 2
MIRT563496 DLGAP3 DLG associated protein 3 2 2
MIRT563890 RAPH1 Ras association (RalGDS/AF-6) and pleckstrin homology domains 1 2 2
MIRT564311 CCNT1 cyclin T1 2 2
MIRT564942 XKR7 XK related 7 2 2
MIRT564979 WNK3 WNK lysine deficient protein kinase 3 2 2
MIRT565423 TEF TEF, PAR bZIP transcription factor 2 2
MIRT566824 MAP3K7 mitogen-activated protein kinase kinase kinase 7 2 2
MIRT571961 KIF5B kinesin family member 5B 2 2
MIRT575877 Cask calcium/calmodulin-dependent serine protein kinase (MAGUK family) 2 3
MIRT576523 Txlna taxilin alpha 2 2
MIRT614693 TRAK1 trafficking kinesin protein 1 2 2
MIRT616065 ZC3H14 zinc finger CCCH-type containing 14 2 2
MIRT618838 ASAH2B N-acylsphingosine amidohydrolase 2B 2 2
MIRT624626 ATXN2 ataxin 2 2 2
MIRT624652 ASAH2 N-acylsphingosine amidohydrolase 2 2 2
MIRT640314 MMAB methylmalonic aciduria (cobalamin deficiency) cblB type 2 2
MIRT659248 CUL3 cullin 3 2 2
MIRT680972 DCAF17 DDB1 and CUL4 associated factor 17 2 2
MIRT682261 RS1 retinoschisin 1 2 2
MIRT682505 GLP2R glucagon like peptide 2 receptor 2 2
MIRT693904 HNRNPA1L2 heterogeneous nuclear ribonucleoprotein A1-like 2 2 2
MIRT699214 SLCO3A1 solute carrier organic anion transporter family member 3A1 2 2
MIRT699373 SLC30A6 solute carrier family 30 member 6 2 2
MIRT699451 SLC16A9 solute carrier family 16 member 9 2 2
MIRT701230 OCRL OCRL, inositol polyphosphate-5-phosphatase 2 2
MIRT702849 HNRNPA1 heterogeneous nuclear ribonucleoprotein A1 2 2
MIRT706163 CASK calcium/calmodulin dependent serine protein kinase 2 3
MIRT718990 UTP15 UTP15, small subunit processome component 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-4524a-5p Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (1500 ng/ml)
hsa-miR-4524a-5p Cetuximab resistant tissue (colorectal carcinoma)

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