pre-miRNA Information
pre-miRNA hsa-mir-4697   
Genomic Coordinates chr11: 133898504 - 133898581
Description Homo sapiens miR-4697 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4697-5p
Sequence 10| AGGGGGCGCAGUCACUGACGUG |31
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs549326907 2 dbSNP
rs897782230 7 dbSNP
rs748950280 8 dbSNP
rs978998780 15 dbSNP
rs968922289 19 dbSNP
rs3802919 20 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol FAM151B   
Synonyms UNQ9217
Description family with sequence similarity 151 member B
Transcript NM_205548   
Expression
Putative miRNA Targets on FAM151B
3'UTR of FAM151B
(miRNA target sites are highlighted)
>FAM151B|NM_205548|3'UTR
   1 GAAGAAGATTCTCAATTATTTCCTGTGTTTTGGTTTCATAATCCTTCTCTCCATTGGTCTGAATTAATTACCATATAAAT
  81 TATGGTTATTGATTGACGTTCCAAGTCATCTAATCAAGAAACGTTTATTGTATGCTTACTCTGTGGGCATATGTCCTTAT
 161 AATAGTGCACTTACATAAAAGATTTGGAAAGAAGAGATTTATTTACACACGTGGCCTAGTCTAATAATAATTCAGGAAAA
 241 TGATACTCTGCACCCCTTCATAAAAATAGTTTTGATAAACTAAAGATGATTGGCAGAATCCATATGTCATAAAACAGGTA
 321 TAAATCACATGTGCTTGGCACTTGCATTACAGAGATTATGAAAAATATGGTACTCTTTTCCTTTTTCCTAGAAAACTGGT
 401 TATTTTAGAGATAACAAAAAAGGAAGAGGGCTTTTAAAATGTTTTTAATTTTCACAGGGGTCAGCAGCTGTCTCATATCA
 481 AGATCTATTAATCCATTTCCTGAAACTGTAGATTAAATTAGATAGATCTAGAATTTCCATGTGATTGAGAAGCATGCTAG
 561 TGGCTAAGTGTTTTTTTTATATTTCTTATTTCTATAGTATCTGATTTATAGTGGACAATATACTGTTGGAGAGCAGTTTT
 641 AGTCTTTGTTATGTATCATTTGTTTTCCAAGTTTCTTACTGCCACCACCTTCATTCAGGTCCTTGTTCTACTAGTAAAAC
 721 TTTATGAACTAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' gugCAGUCACUGAC-GCGGGGga 5'
             ||:| |||:|| ||::||  
Target 5' atgGTTATTGATTGACGTTCCaa 3'
82 - 104 119.00 -17.80
2
miRNA  3' gugcAGUC-----ACU----GACGCGGGGGa 5'
              ||||     |||    |||| ||||: 
Target 5' taatTCAGGAAAATGATACTCTGCACCCCTt 3'
228 - 258 112.00 -17.20
3
miRNA  3' gugCAGUCACUGACGCGGGGGa 5'
             ||:  | ||  :||| || 
Target 5' caaGTTTCTTAC--TGCCACCa 3'
668 - 687 99.00 -9.80
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30129479 12 COSMIC
COSN31558966 16 COSMIC
COSN30456643 23 COSMIC
COSN31604526 23 COSMIC
COSN30150370 38 COSMIC
COSN31587589 50 COSMIC
COSN31502514 52 COSMIC
COSN30126650 54 COSMIC
COSN22011527 62 COSMIC
COSN30154091 92 COSMIC
COSN31529496 181 COSMIC
COSN26154581 229 COSMIC
COSN17182120 338 COSMIC
COSN6928187 339 COSMIC
COSN8386435 458 COSMIC
COSN28198263 526 COSMIC
COSN17934460 729 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs771330562 1 dbSNP
rs1323894812 2 dbSNP
rs542011842 10 dbSNP
rs759821618 13 dbSNP
rs765376708 19 dbSNP
rs1290141913 26 dbSNP
rs775444232 27 dbSNP
rs1411291160 33 dbSNP
rs763037726 41 dbSNP
rs767387859 49 dbSNP
rs1240106317 51 dbSNP
rs1376956144 54 dbSNP
rs1310884019 58 dbSNP
rs1384706815 59 dbSNP
rs995274741 66 dbSNP
rs1215715475 67 dbSNP
rs905330027 74 dbSNP
rs1249132799 76 dbSNP
rs879250560 77 dbSNP
rs1466630740 81 dbSNP
rs1358413199 86 dbSNP
rs1028461476 92 dbSNP
rs1181898323 94 dbSNP
rs186380131 98 dbSNP
rs1445523225 99 dbSNP
rs1183768323 110 dbSNP
rs1184147935 116 dbSNP
rs545776704 123 dbSNP
rs1398668249 124 dbSNP
rs1158308038 130 dbSNP
rs1192020791 133 dbSNP
rs1489649171 150 dbSNP
rs1284379045 152 dbSNP
rs1216496796 160 dbSNP
rs1355126045 162 dbSNP
rs953876874 169 dbSNP
rs892539564 175 dbSNP
rs1009643921 186 dbSNP
rs1301473003 197 dbSNP
rs986661441 207 dbSNP
rs1019572162 211 dbSNP
rs965354808 212 dbSNP
rs1400520013 219 dbSNP
rs975991559 231 dbSNP
rs1464567673 243 dbSNP
rs1377173455 246 dbSNP
rs1177023486 247 dbSNP
rs1381370799 249 dbSNP
rs912393189 255 dbSNP
rs953215447 268 dbSNP
rs1448326224 269 dbSNP
rs527969769 274 dbSNP
rs1488822534 287 dbSNP
rs139154523 291 dbSNP
rs1285547392 297 dbSNP
rs1199263252 301 dbSNP
rs978375068 305 dbSNP
rs1489851760 309 dbSNP
rs1343616404 319 dbSNP
rs760102285 330 dbSNP
rs1256116007 331 dbSNP
rs924645820 335 dbSNP
rs940419121 346 dbSNP
rs1277326885 349 dbSNP
rs190846988 352 dbSNP
rs1331897021 355 dbSNP
rs917553634 368 dbSNP
rs1054566259 373 dbSNP
rs949043393 376 dbSNP
rs916006579 386 dbSNP
rs1401560421 387 dbSNP
rs1303560035 389 dbSNP
rs1189885370 394 dbSNP
rs1471078337 398 dbSNP
rs948791961 403 dbSNP
rs1045910055 407 dbSNP
rs1478322925 416 dbSNP
rs907376307 417 dbSNP
rs1186004719 418 dbSNP
rs115102419 424 dbSNP
rs1256223450 426 dbSNP
rs115931396 429 dbSNP
rs1256977014 430 dbSNP
rs1218896744 431 dbSNP
rs375263325 436 dbSNP
rs1473558188 454 dbSNP
rs1054357434 457 dbSNP
rs1404757059 458 dbSNP
rs1341066250 472 dbSNP
rs570009796 479 dbSNP
rs892677328 488 dbSNP
rs1352473274 500 dbSNP
rs770616603 504 dbSNP
rs1466371028 507 dbSNP
rs145191880 509 dbSNP
rs889574962 511 dbSNP
rs1222258364 519 dbSNP
rs1007979685 530 dbSNP
rs1369485160 560 dbSNP
rs1041120246 561 dbSNP
rs1437236416 561 dbSNP
rs901154493 565 dbSNP
rs1287467910 571 dbSNP
rs528344560 571 dbSNP
rs57294326 571 dbSNP
rs182650735 572 dbSNP
rs1208951812 575 dbSNP
rs750511054 579 dbSNP
rs1267088040 582 dbSNP
rs1006177160 586 dbSNP
rs978050223 588 dbSNP
rs1032652873 591 dbSNP
rs1202305329 598 dbSNP
rs1270965923 602 dbSNP
rs534898108 603 dbSNP
rs1337823123 607 dbSNP
rs187748310 610 dbSNP
rs1321833530 619 dbSNP
rs1405076516 620 dbSNP
rs915902879 627 dbSNP
rs576102386 635 dbSNP
rs577735486 648 dbSNP
rs1379141620 653 dbSNP
rs1178788311 654 dbSNP
rs543408057 671 dbSNP
rs115090899 672 dbSNP
rs980830037 676 dbSNP
rs544401292 682 dbSNP
rs1293718851 687 dbSNP
rs190959488 690 dbSNP
rs940155003 691 dbSNP
rs1478314332 700 dbSNP
rs1285390029 711 dbSNP
rs1037303110 724 dbSNP
rs759047626 727 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084065. RNA binding protein: AGO2. Condition:CLIP_emetine_AbnovaAb HITS-CLIP data was present in GSM1084077. RNA binding protein: AGO2. Condition:CLIP_hippuristanol_rep1_SigmaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' gugcagucacugacgCGGGGGa 5'
                         |||||| 
Target 5' -----------cucaGCCCCCa 3'
1 - 11
Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084065
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_emetine_AbnovaAb
Location of target site ENST00000282226.4 | 3UTR | CAGCCCCCAAAGUGCUGGGAUU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM1084077
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_hippuristanol_rep1_SigmaAb
Location of target site ENST00000282226.4 | 3UTR | CUCAGCCCCCAAAGUGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
67 hsa-miR-4697-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT441332 C19orf26 CACN beta subunit associated regulatory protein 2 4
MIRT451390 FARSA phenylalanyl-tRNA synthetase alpha subunit 2 2
MIRT452310 EIF5AL1 eukaryotic translation initiation factor 5A-like 1 2 2
MIRT455043 MEN1 menin 1 2 2
MIRT455256 DDX39B DExD-box helicase 39B 2 10
MIRT461279 COX10 COX10, heme A:farnesyltransferase cytochrome c oxidase assembly factor 2 2
MIRT464945 TXLNA taxilin alpha 2 4
MIRT468043 SIK1 salt inducible kinase 1 2 2
MIRT472523 NACC1 nucleus accumbens associated 1 2 2
MIRT472929 MSN moesin 2 2
MIRT473247 MIDN midnolin 2 2
MIRT475828 HDGF heparin binding growth factor 2 2
MIRT478744 CS citrate synthase 2 2
MIRT480081 CALR calreticulin 2 2
MIRT483482 STMN3 stathmin 3 2 4
MIRT483643 QSOX2 quiescin sulfhydryl oxidase 2 2 4
MIRT483721 THSD4 thrombospondin type 1 domain containing 4 2 2
MIRT484539 BARHL1 BarH like homeobox 1 2 6
MIRT486041 WSCD1 WSC domain containing 1 2 4
MIRT486142 SIX5 SIX homeobox 5 2 6
MIRT486498 MYH11 myosin heavy chain 11 2 2
MIRT486977 STEAP3 STEAP3 metalloreductase 2 4
MIRT487279 AGPAT6 glycerol-3-phosphate acyltransferase 4 2 4
MIRT487742 MIB2 mindbomb E3 ubiquitin protein ligase 2 2 2
MIRT487987 RXRB retinoid X receptor beta 2 2
MIRT488338 PAX2 paired box 2 2 2
MIRT488668 WWP2 WW domain containing E3 ubiquitin protein ligase 2 2 4
MIRT488752 FXYD1 FXYD domain containing ion transport regulator 1 2 2
MIRT488812 TBC1D28 TBC1 domain family member 28 2 2
MIRT489380 RAB11B RAB11B, member RAS oncogene family 2 2
MIRT489744 TACC3 transforming acidic coiled-coil containing protein 3 2 2
MIRT489960 GNB2 G protein subunit beta 2 2 2
MIRT490418 VPS51 VPS51, GARP complex subunit 2 4
MIRT490639 FEM1A fem-1 homolog A 2 2
MIRT491086 MSI1 musashi RNA binding protein 1 2 4
MIRT491298 VGF VGF nerve growth factor inducible 2 4
MIRT491366 SLC12A5 solute carrier family 12 member 5 2 2
MIRT492464 RASD1 ras related dexamethasone induced 1 2 4
MIRT492872 NFIX nuclear factor I X 2 2
MIRT492951 NEUROD2 neuronal differentiation 2 2 2
MIRT493704 H2AFX H2A histone family member X 2 2
MIRT493977 EIF1 eukaryotic translation initiation factor 1 2 4
MIRT500360 ZNF385A zinc finger protein 385A 2 2
MIRT501154 SLC10A7 solute carrier family 10 member 7 2 6
MIRT509625 RRP7A ribosomal RNA processing 7 homolog A 2 4
MIRT512232 ATG2A autophagy related 2A 2 8
MIRT529822 ARGFX arginine-fifty homeobox 2 4
MIRT531179 SIGLEC12 sialic acid binding Ig like lectin 12 (gene/pseudogene) 2 2
MIRT538971 BCL7A BCL tumor suppressor 7A 2 2
MIRT548357 ENTPD5 ectonucleoside triphosphate diphosphohydrolase 5 2 4
MIRT553634 TJAP1 tight junction associated protein 1 2 2
MIRT558050 EVI5L ecotropic viral integration site 5 like 2 2
MIRT562548 CCDC71L coiled-coil domain containing 71 like 2 4
MIRT568941 RUNX3 runt related transcription factor 3 2 2
MIRT569114 ONECUT3 one cut homeobox 3 2 2
MIRT573591 CERS1 ceramide synthase 1 2 2
MIRT619317 ARHGAP18 Rho GTPase activating protein 18 2 2
MIRT621202 ARPC1B actin related protein 2/3 complex subunit 1B 2 2
MIRT628844 FAM151B family with sequence similarity 151 member B 2 2
MIRT670497 LYRM4 LYR motif containing 4 2 2
MIRT670545 SHISA2 shisa family member 2 2 2
MIRT671026 PCDHB2 protocadherin beta 2 2 2
MIRT688989 ATP6AP1 ATPase H+ transporting accessory protein 1 2 2
MIRT709275 MAPK8IP2 mitogen-activated protein kinase 8 interacting protein 2 2 2
MIRT715084 ELOF1 elongation factor 1 homolog 2 2
MIRT718348 NPBWR1 neuropeptides B and W receptor 1 2 2
MIRT737406 MMP7 matrix metallopeptidase 7 2 0
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-4697 Ceritinib 57379345 NSC776422 approved sensitive cell line (H3122)
hsa-miR-4697-5p Fulvestrant 17756771 NSC719276 approved resistant High Breast Cancer cell line (MCF-7)
hsa-miR-4697-5p Paclitaxel 36314 NSC125973 approved resistant High Breast Cancer cell line (Bads-200, Bats-72, BCap37)
hsa-miR-4697-5p Cisplatin 5460033 NSC119875 approved sensitive cell line (CAL-27) (total RNA)
hsa-miR-4697-5p Osimertinib 71496458 NSC779217 approved resistant cell line (H1975)
hsa-miR-4697-5p Cisplatin 5460033 NSC119875 approved resistant cell line (MGC-803)
hsa-miR-4697-5p Cisplatin 5460033 NSC119875 approved sensitive cell line (A2780)
hsa-miR-4697-5p Neoadjuvant chemotherapy resistant tissue (breast cancer)
hsa-miR-4697-5p Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (100 ng/ml)
hsa-miR-4697-5p Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (1500 ng/ml)

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