pre-miRNA Information | |
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pre-miRNA | hsa-mir-4697 |
Genomic Coordinates | chr11: 133898504 - 133898581 |
Description | Homo sapiens miR-4697 stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Mature miRNA Information | ||||||||||||||||||||||
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Mature miRNA | hsa-miR-4697-5p | |||||||||||||||||||||
Sequence | 10| AGGGGGCGCAGUCACUGACGUG |31 | |||||||||||||||||||||
Evidence | Experimental | |||||||||||||||||||||
Experiments | Illumina | |||||||||||||||||||||
SNPs in miRNA |
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Putative Targets |
miRNA Expression profile | |
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Human miRNA Tissue Atlas | |
Circulating MicroRNA Expression Profiling |
Gene Information | |||||||||||||||||||||
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Gene Symbol | FAM151B | ||||||||||||||||||||
Synonyms | UNQ9217 | ||||||||||||||||||||
Description | family with sequence similarity 151 member B | ||||||||||||||||||||
Transcript | NM_205548 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on FAM151B | |||||||||||||||||||||
3'UTR of FAM151B (miRNA target sites are highlighted) |
>FAM151B|NM_205548|3'UTR 1 GAAGAAGATTCTCAATTATTTCCTGTGTTTTGGTTTCATAATCCTTCTCTCCATTGGTCTGAATTAATTACCATATAAAT 81 TATGGTTATTGATTGACGTTCCAAGTCATCTAATCAAGAAACGTTTATTGTATGCTTACTCTGTGGGCATATGTCCTTAT 161 AATAGTGCACTTACATAAAAGATTTGGAAAGAAGAGATTTATTTACACACGTGGCCTAGTCTAATAATAATTCAGGAAAA 241 TGATACTCTGCACCCCTTCATAAAAATAGTTTTGATAAACTAAAGATGATTGGCAGAATCCATATGTCATAAAACAGGTA 321 TAAATCACATGTGCTTGGCACTTGCATTACAGAGATTATGAAAAATATGGTACTCTTTTCCTTTTTCCTAGAAAACTGGT 401 TATTTTAGAGATAACAAAAAAGGAAGAGGGCTTTTAAAATGTTTTTAATTTTCACAGGGGTCAGCAGCTGTCTCATATCA 481 AGATCTATTAATCCATTTCCTGAAACTGTAGATTAAATTAGATAGATCTAGAATTTCCATGTGATTGAGAAGCATGCTAG 561 TGGCTAAGTGTTTTTTTTATATTTCTTATTTCTATAGTATCTGATTTATAGTGGACAATATACTGTTGGAGAGCAGTTTT 641 AGTCTTTGTTATGTATCATTTGTTTTCCAAGTTTCTTACTGCCACCACCTTCATTCAGGTCCTTGTTCTACTAGTAAAAC 721 TTTATGAACTAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
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miRNA:Target | ---- | ||||||
Validation Method |
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Conditions | HEK293S | ||||||
Location of target site | 3'UTR | ||||||
Tools used in this research | TargetScan , miRTarCLIP , Piranha | ||||||
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM1084065. RNA binding protein: AGO2. Condition:CLIP_emetine_AbnovaAb
HITS-CLIP data was present in GSM1084077. RNA binding protein: AGO2. Condition:CLIP_hippuristanol_rep1_SigmaAb
... - Karginov FV; Hannon GJ, 2013, Genes & development. |
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miRNA-target interactions (Provided by authors) |
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Article |
- Karginov FV; Hannon GJ - Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
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CLIP-seq Support 1 for dataset GSM1084065 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_emetine_AbnovaAb |
Location of target site | ENST00000282226.4 | 3UTR | CAGCCCCCAAAGUGCUGGGAUU |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset GSM1084077 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_hippuristanol_rep1_SigmaAb |
Location of target site | ENST00000282226.4 | 3UTR | CUCAGCCCCCAAAGUGC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
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MiRNA-Target Expression Profile (TCGA) | |||||||
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67 hsa-miR-4697-5p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT441332 | C19orf26 | CACN beta subunit associated regulatory protein | ![]() |
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2 | 4 | ||||||
MIRT451390 | FARSA | phenylalanyl-tRNA synthetase alpha subunit | ![]() |
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2 | 2 | ||||||
MIRT452310 | EIF5AL1 | eukaryotic translation initiation factor 5A-like 1 | ![]() |
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2 | 2 | ||||||
MIRT455043 | MEN1 | menin 1 | ![]() |
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2 | 2 | ||||||
MIRT455256 | DDX39B | DExD-box helicase 39B | ![]() |
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2 | 10 | ||||||
MIRT461279 | COX10 | COX10, heme A:farnesyltransferase cytochrome c oxidase assembly factor | ![]() |
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2 | 2 | ||||||
MIRT464945 | TXLNA | taxilin alpha | ![]() |
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2 | 4 | ||||||
MIRT468043 | SIK1 | salt inducible kinase 1 | ![]() |
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2 | 2 | ||||||
MIRT472523 | NACC1 | nucleus accumbens associated 1 | ![]() |
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2 | 2 | ||||||
MIRT472929 | MSN | moesin | ![]() |
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2 | 2 | ||||||
MIRT473247 | MIDN | midnolin | ![]() |
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2 | 2 | ||||||
MIRT475828 | HDGF | heparin binding growth factor | ![]() |
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2 | 2 | ||||||
MIRT478744 | CS | citrate synthase | ![]() |
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2 | 2 | ||||||
MIRT480081 | CALR | calreticulin | ![]() |
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2 | 2 | ||||||
MIRT483482 | STMN3 | stathmin 3 | ![]() |
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2 | 4 | ||||||
MIRT483643 | QSOX2 | quiescin sulfhydryl oxidase 2 | ![]() |
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2 | 4 | ||||||
MIRT483721 | THSD4 | thrombospondin type 1 domain containing 4 | ![]() |
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2 | 2 | ||||||
MIRT484539 | BARHL1 | BarH like homeobox 1 | ![]() |
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2 | 6 | ||||||
MIRT486041 | WSCD1 | WSC domain containing 1 | ![]() |
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2 | 4 | ||||||
MIRT486142 | SIX5 | SIX homeobox 5 | ![]() |
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2 | 6 | ||||||
MIRT486498 | MYH11 | myosin heavy chain 11 | ![]() |
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2 | 2 | ||||||
MIRT486977 | STEAP3 | STEAP3 metalloreductase | ![]() |
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2 | 4 | ||||||
MIRT487279 | AGPAT6 | glycerol-3-phosphate acyltransferase 4 | ![]() |
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2 | 4 | ||||||
MIRT487742 | MIB2 | mindbomb E3 ubiquitin protein ligase 2 | ![]() |
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2 | 2 | ||||||
MIRT487987 | RXRB | retinoid X receptor beta | ![]() |
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2 | 2 | ||||||
MIRT488338 | PAX2 | paired box 2 | ![]() |
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2 | 2 | ||||||
MIRT488668 | WWP2 | WW domain containing E3 ubiquitin protein ligase 2 | ![]() |
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2 | 4 | ||||||
MIRT488752 | FXYD1 | FXYD domain containing ion transport regulator 1 | ![]() |
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2 | 2 | ||||||
MIRT488812 | TBC1D28 | TBC1 domain family member 28 | ![]() |
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2 | 2 | ||||||
MIRT489380 | RAB11B | RAB11B, member RAS oncogene family | ![]() |
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2 | 2 | ||||||
MIRT489744 | TACC3 | transforming acidic coiled-coil containing protein 3 | ![]() |
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2 | 2 | ||||||
MIRT489960 | GNB2 | G protein subunit beta 2 | ![]() |
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2 | 2 | ||||||
MIRT490418 | VPS51 | VPS51, GARP complex subunit | ![]() |
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2 | 4 | ||||||
MIRT490639 | FEM1A | fem-1 homolog A | ![]() |
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2 | 2 | ||||||
MIRT491086 | MSI1 | musashi RNA binding protein 1 | ![]() |
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2 | 4 | ||||||
MIRT491298 | VGF | VGF nerve growth factor inducible | ![]() |
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2 | 4 | ||||||
MIRT491366 | SLC12A5 | solute carrier family 12 member 5 | ![]() |
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2 | 2 | ||||||
MIRT492464 | RASD1 | ras related dexamethasone induced 1 | ![]() |
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2 | 4 | ||||||
MIRT492872 | NFIX | nuclear factor I X | ![]() |
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2 | 2 | ||||||
MIRT492951 | NEUROD2 | neuronal differentiation 2 | ![]() |
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2 | 2 | ||||||
MIRT493704 | H2AFX | H2A histone family member X | ![]() |
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2 | 2 | ||||||
MIRT493977 | EIF1 | eukaryotic translation initiation factor 1 | ![]() |
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2 | 4 | ||||||
MIRT500360 | ZNF385A | zinc finger protein 385A | ![]() |
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2 | 2 | ||||||
MIRT501154 | SLC10A7 | solute carrier family 10 member 7 | ![]() |
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2 | 6 | ||||||
MIRT509625 | RRP7A | ribosomal RNA processing 7 homolog A | ![]() |
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2 | 4 | ||||||
MIRT512232 | ATG2A | autophagy related 2A | ![]() |
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2 | 8 | ||||||
MIRT529822 | ARGFX | arginine-fifty homeobox | ![]() |
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2 | 4 | ||||||
MIRT531179 | SIGLEC12 | sialic acid binding Ig like lectin 12 (gene/pseudogene) | ![]() |
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2 | 2 | ||||||
MIRT538971 | BCL7A | BCL tumor suppressor 7A | ![]() |
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2 | 2 | ||||||
MIRT548357 | ENTPD5 | ectonucleoside triphosphate diphosphohydrolase 5 | ![]() |
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2 | 4 | ||||||
MIRT553634 | TJAP1 | tight junction associated protein 1 | ![]() |
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2 | 2 | ||||||
MIRT558050 | EVI5L | ecotropic viral integration site 5 like | ![]() |
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2 | 2 | ||||||
MIRT562548 | CCDC71L | coiled-coil domain containing 71 like | ![]() |
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2 | 4 | ||||||
MIRT568941 | RUNX3 | runt related transcription factor 3 | ![]() |
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2 | 2 | ||||||
MIRT569114 | ONECUT3 | one cut homeobox 3 | ![]() |
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2 | 2 | ||||||
MIRT573591 | CERS1 | ceramide synthase 1 | ![]() |
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2 | 2 | ||||||
MIRT619317 | ARHGAP18 | Rho GTPase activating protein 18 | ![]() |
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2 | 2 | ||||||
MIRT621202 | ARPC1B | actin related protein 2/3 complex subunit 1B | ![]() |
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2 | 2 | ||||||
MIRT628844 | FAM151B | family with sequence similarity 151 member B | ![]() |
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2 | 2 | ||||||
MIRT670497 | LYRM4 | LYR motif containing 4 | ![]() |
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2 | 2 | ||||||
MIRT670545 | SHISA2 | shisa family member 2 | ![]() |
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2 | 2 | ||||||
MIRT671026 | PCDHB2 | protocadherin beta 2 | ![]() |
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2 | 2 | ||||||
MIRT688989 | ATP6AP1 | ATPase H+ transporting accessory protein 1 | ![]() |
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2 | 2 | ||||||
MIRT709275 | MAPK8IP2 | mitogen-activated protein kinase 8 interacting protein 2 | ![]() |
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2 | 2 | ||||||
MIRT715084 | ELOF1 | elongation factor 1 homolog | ![]() |
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2 | 2 | ||||||
MIRT718348 | NPBWR1 | neuropeptides B and W receptor 1 | ![]() |
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2 | 2 | ||||||
MIRT737406 | MMP7 | matrix metallopeptidase 7 | ![]() |
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2 | 0 |
miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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