pre-miRNA Information
pre-miRNA hsa-mir-5691   
Genomic Coordinates chr11: 9090312 - 9090379
Description Homo sapiens miR-5691 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-5691
Sequence 9| UUGCUCUGAGCUCCGAGAAAGC |30
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1042238449 3 dbSNP
rs554804494 11 dbSNP
rs1172229307 13 dbSNP
rs112511786 14 dbSNP
Putative Targets

Gene Information
Gene Symbol GSN   
Synonyms ADF, AGEL
Description gelsolin
Transcript NM_000177   
Other Transcripts NM_001127662 , NM_001127663 , NM_001127664 , NM_001127665 , NM_001127666 , NM_001127667 , NM_198252   
Expression
Putative miRNA Targets on GSN
3'UTR of GSN
(miRNA target sites are highlighted)
>GSN|NM_000177|3'UTR
   1 GGAGGGGCAGGGCCCACCCATGTCACCGGTCAGTGCCTTTTGGAACTGTCCTTCCCTCAAAGAGGCCTTAGAGCGAGCAG
  81 AGCAGCTCTGCTATGAGTGTGTGTGTGTGTGTGTGTTGTTTCTTTTTTTTTTTTTTACAGTATCCAAAAATAGCCCTGCA
 161 AAAATTCAGAGTCCTTGCAAAATTGTCTAAAATGTCAGTGTTTGGGAAATTAAATCCAATAAAAACATTTTGAAGTGTGA
 241 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' cgaaaGAGCCUCG--AGUCUCGUu 5'
               ||: ||||   ||||||| 
Target 5' gaggcCTTAGAGCGAGCAGAGCAg 3'
62 - 85 155.00 -18.80
2
miRNA  3' cgaaagagccUCGAGUCUCGuu 5'
                    | :||||||:  
Target 5' gccctgcaaaAATTCAGAGTcc 3'
153 - 174 112.00 -8.10
3
miRNA  3' cgaaagagCCU-CGAGUCUCGuu 5'
                  ||| |  |||:||  
Target 5' --------GGAGGGGCAGGGCcc 3'
1 - 15 110.00 -11.80
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
915197 29 ClinVar
364831 97 ClinVar
364832 97 ClinVar
364833 97 ClinVar
364834 97 ClinVar
912486 99 ClinVar
364835 117 ClinVar
364836 117 ClinVar
364837 121 ClinVar
364838 123 ClinVar
364839 125 ClinVar
364840 127 ClinVar
364841 186 ClinVar
364842 195 ClinVar
364843 206 ClinVar
COSN30169330 9 COSMIC
COSN30501596 23 COSMIC
COSN28127729 34 COSMIC
COSN27272972 117 COSMIC
COSN18719934 119 COSMIC
COSN27296704 136 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1406292881 5 dbSNP
rs539998674 6 dbSNP
rs1421376273 10 dbSNP
rs1160410677 11 dbSNP
rs754155730 12 dbSNP
rs1358479520 14 dbSNP
rs756688937 16 dbSNP
rs781360178 18 dbSNP
rs753134056 21 dbSNP
rs1445666383 25 dbSNP
rs568601953 27 dbSNP
rs371549019 28 dbSNP
rs376801564 29 dbSNP
rs1224151536 30 dbSNP
rs771042562 38 dbSNP
rs780246906 47 dbSNP
rs778890943 49 dbSNP
rs1265150944 51 dbSNP
rs1001838675 61 dbSNP
rs1300920522 61 dbSNP
rs1051142 63 dbSNP
rs1218709506 70 dbSNP
rs1423674465 70 dbSNP
rs865914463 73 dbSNP
rs374518331 74 dbSNP
rs143181937 75 dbSNP
rs557506583 76 dbSNP
rs1474703033 80 dbSNP
rs1373028953 85 dbSNP
rs1051174 89 dbSNP
rs1193937465 94 dbSNP
rs147410423 97 dbSNP
rs757946835 97 dbSNP
rs760072243 97 dbSNP
rs886063408 97 dbSNP
rs1247683945 98 dbSNP
rs903037306 99 dbSNP
rs1229869161 104 dbSNP
rs1488398937 106 dbSNP
rs999153987 110 dbSNP
rs1218653012 114 dbSNP
rs1341823208 116 dbSNP
rs751044080 117 dbSNP
rs1224984417 118 dbSNP
rs1273562539 119 dbSNP
rs1265635253 120 dbSNP
rs1350163292 120 dbSNP
rs1403921617 120 dbSNP
rs886063409 121 dbSNP
rs1196505809 123 dbSNP
rs1325890106 123 dbSNP
rs15034 123 dbSNP
rs377469209 123 dbSNP
rs71680051 123 dbSNP
rs750556612 123 dbSNP
rs1479119993 125 dbSNP
rs886063410 125 dbSNP
rs886063411 127 dbSNP
rs1193081395 130 dbSNP
rs1198840124 137 dbSNP
rs1480873294 138 dbSNP
rs1248706717 139 dbSNP
rs73660440 141 dbSNP
rs11550202 143 dbSNP
rs951519707 154 dbSNP
rs1425609945 156 dbSNP
rs1199192888 160 dbSNP
rs1319969716 168 dbSNP
rs1280787957 178 dbSNP
rs1222400423 180 dbSNP
rs757682798 186 dbSNP
rs1051286 188 dbSNP
rs886063412 195 dbSNP
rs1368971014 204 dbSNP
rs1335530398 205 dbSNP
rs779431879 206 dbSNP
rs1007266095 207 dbSNP
rs746309321 222 dbSNP
rs1404597865 226 dbSNP
rs1480235147 236 dbSNP
rs963152920 240 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084076. RNA binding protein: AGO2. Condition:CLIP_nohippuristanol_rep1_SigmaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' cgaaagagccucgagUCUCGUu 5'
                         |||||| 
Target 5' ---------------AGAGCAg 3'
1 - 7
Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084076
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_nohippuristanol_rep1_SigmaAb
Location of target site ENST00000373823.3 | 3UTR | AGAGCAGCUCUGCUAUGAGUGUGUGUGUGUGUGUGUGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
91 hsa-miR-5691 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT091162 TBL1XR1 transducin beta like 1 X-linked receptor 1 2 2
MIRT242227 TTC9 tetratricopeptide repeat domain 9 2 4
MIRT246192 TXNIP thioredoxin interacting protein 2 2
MIRT251553 DCAF7 DDB1 and CUL4 associated factor 7 2 4
MIRT387267 SOX9 SRY-box 9 2 2
MIRT463486 ZC3H11A zinc finger CCCH-type containing 11A 2 12
MIRT494446 BTG2 BTG anti-proliferation factor 2 2 2
MIRT496478 ADAMTS17 ADAM metallopeptidase with thrombospondin type 1 motif 17 2 2
MIRT501825 NCOA3 nuclear receptor coactivator 3 2 2
MIRT503072 C6orf120 chromosome 6 open reading frame 120 2 2
MIRT504755 TEP1 telomerase associated protein 1 2 4
MIRT505971 RAB11FIP1 RAB11 family interacting protein 1 2 4
MIRT511868 GOLGA7 golgin A7 2 6
MIRT512900 UBL4A ubiquitin like 4A 2 2
MIRT513048 LYPD6 LY6/PLAUR domain containing 6 2 6
MIRT523772 FAM83D family with sequence similarity 83 member D 2 2
MIRT525126 RPS11 ribosomal protein S11 2 2
MIRT525816 VIMP selenoprotein S 2 10
MIRT531222 HIST1H2BD histone cluster 1 H2B family member d 2 2
MIRT531729 SLC2A9 solute carrier family 2 member 9 2 2
MIRT536282 LIMA1 LIM domain and actin binding 1 2 2
MIRT537291 G3BP1 G3BP stress granule assembly factor 1 2 2
MIRT537724 ELAVL2 ELAV like RNA binding protein 2 2 2
MIRT547940 HNRNPA0 heterogeneous nuclear ribonucleoprotein A0 2 2
MIRT554177 SLC35E2B solute carrier family 35 member E2B 2 2
MIRT555015 RAB2B RAB2B, member RAS oncogene family 2 2
MIRT576717 Wars tryptophanyl-tRNA synthetase 2 2
MIRT607484 HEBP2 heme binding protein 2 2 2
MIRT610786 KLK2 kallikrein related peptidase 2 2 2
MIRT611491 ZNF440 zinc finger protein 440 2 2
MIRT613986 DBT dihydrolipoamide branched chain transacylase E2 2 2
MIRT615782 KIAA0319L KIAA0319 like 2 2
MIRT620563 WBSCR27 methyltransferase like 27 2 4
MIRT624157 DGKE diacylglycerol kinase epsilon 2 2
MIRT626088 MKLN1 muskelin 1 2 2
MIRT629481 GSN gelsolin 2 2
MIRT636645 CDK4 cyclin dependent kinase 4 2 2
MIRT638074 HS3ST1 heparan sulfate-glucosamine 3-sulfotransferase 1 2 2
MIRT638442 PLXDC2 plexin domain containing 2 2 2
MIRT641025 KLHL7 kelch like family member 7 2 2
MIRT641539 MOCOS molybdenum cofactor sulfurase 2 2
MIRT643004 ZNF829 zinc finger protein 829 2 2
MIRT643092 NDUFB5 NADH:ubiquinone oxidoreductase subunit B5 2 2
MIRT644979 IL2RA interleukin 2 receptor subunit alpha 2 2
MIRT645912 PLXNA3 plexin A3 2 2
MIRT646605 ORAI2 ORAI calcium release-activated calcium modulator 2 2 2
MIRT648033 FADS6 fatty acid desaturase 6 2 2
MIRT657491 HBEGF heparin binding EGF like growth factor 2 2
MIRT658381 FAM26E calcium homeostasis modulator family member 5 2 2
MIRT659695 CD200 CD200 molecule 2 2
MIRT661276 TEX9 testis expressed 9 2 2
MIRT661348 DYRK4 dual specificity tyrosine phosphorylation regulated kinase 4 2 2
MIRT662948 JPH2 junctophilin 2 2 2
MIRT663225 PPY pancreatic polypeptide 2 2
MIRT663280 SPN sialophorin 2 2
MIRT663335 ZNF74 zinc finger protein 74 2 2
MIRT664344 C16orf45 chromosome 16 open reading frame 45 2 2
MIRT664855 TBRG4 transforming growth factor beta regulator 4 2 2
MIRT665484 VPS53 VPS53, GARP complex subunit 2 2
MIRT666428 SH2B3 SH2B adaptor protein 3 2 2
MIRT668162 GDE1 glycerophosphodiester phosphodiesterase 1 2 2
MIRT670170 CCDC142 coiled-coil domain containing 142 2 2
MIRT671277 MTO1 mitochondrial tRNA translation optimization 1 2 2
MIRT672284 GP2 glycoprotein 2 2 2
MIRT672435 RAB10 RAB10, member RAS oncogene family 2 2
MIRT672646 SLC25A16 solute carrier family 25 member 16 2 4
MIRT672760 UBE2V2 ubiquitin conjugating enzyme E2 V2 2 2
MIRT672834 AKR7L aldo-keto reductase family 7 like (gene/pseudogene) 2 2
MIRT672841 ICOSLG inducible T-cell costimulator ligand 2 2
MIRT673080 AK1 adenylate kinase 1 2 2
MIRT673148 C1orf50 chromosome 1 open reading frame 50 2 2
MIRT673323 THAP1 THAP domain containing 1 2 2
MIRT673893 DCTN6 dynactin subunit 6 2 2
MIRT674182 PLEKHM3 pleckstrin homology domain containing M3 2 2
MIRT674607 RBBP4 RB binding protein 4, chromatin remodeling factor 2 2
MIRT674740 SLC16A1 solute carrier family 16 member 1 2 2
MIRT675140 MOGAT1 monoacylglycerol O-acyltransferase 1 2 4
MIRT675194 NKPD1 NTPase KAP family P-loop domain containing 1 2 2
MIRT675886 SNAP29 synaptosome associated protein 29 2 2
MIRT679163 PSMB2 proteasome subunit beta 2 2 2
MIRT680337 ZNF281 zinc finger protein 281 2 2
MIRT706699 GPR155 G protein-coupled receptor 155 2 2
MIRT706911 THAP6 THAP domain containing 6 2 2
MIRT707880 SLC45A4 solute carrier family 45 member 4 2 2
MIRT709097 SEPT4 septin 4 2 2
MIRT709408 FBXL20 F-box and leucine rich repeat protein 20 2 2
MIRT710845 FAM210A family with sequence similarity 210 member A 2 2
MIRT711358 VPS8 VPS8, CORVET complex subunit 2 2
MIRT718590 SCD5 stearoyl-CoA desaturase 5 2 2
MIRT723649 RPTN repetin 2 2
MIRT724208 NUP205 nucleoporin 205 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-5691 Vemurafenib 42611257 NSC761431 approved sensitive cell line (451Lu)

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