pre-miRNA Information
pre-miRNA hsa-mir-6819   
Genomic Coordinates chr22: 36286847 - 36286907
Description Homo sapiens miR-6819 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-6819-3p
Sequence 41| AAGCCUCUGUCCCCACCCCAG |61
Evidence Experimental
Experiments Meta-analysis
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1279530967 2 dbSNP
rs967579979 3 dbSNP
rs776891214 4 dbSNP
rs771166635 8 dbSNP
rs747037324 9 dbSNP
rs879248090 11 dbSNP
rs1485325191 12 dbSNP
rs778157316 16 dbSNP
Putative Targets

Gene Information
Gene Symbol ZNF91   
Synonyms HPF7, HTF10
Description zinc finger protein 91
Transcript NM_003430   
Expression
Putative miRNA Targets on ZNF91
3'UTR of ZNF91
(miRNA target sites are highlighted)
>ZNF91|NM_003430|3'UTR
   1 AAAATACAAAAAATTAGCCGGGCGTGGTGGCGGGCGCCTGTAGTCCCAGCTATGCGAGAGGCTGAGGCAGGAGAATGGCG
  81 TGAACCCGGGAGGCGGAGCTTGCAGTGAGCCGAGATCACGCCACTGCACTCAAGCCTGGGCGACAGAGCGAGACTCCGTC
 161 TCAAAAAAAAAAAAAAGAAAATTCATACTAGAGAGAAACCCTACAAATATAAAGAATGTGGCAAATCTTTTAACCGGTCT
 241 TCAACCTTTACTAAACATAAGGTAATTCATACTGGAGTAAAACTCTACAAATGTGAAGAATGTGGCAAATCCTTTTTCTG
 321 GTCCTCAGCCCTAACCAGACATAAGAAAATTCATACTGGACAGCAACCCTACAAACAGGAAAAATTTGGCAAAGCCTTTA
 401 ACCAGTTTTCACACCTTACTACAGATAAGATAACTCATATTGGAGAGAAATCTTACAAGTGTGAATAATGTGGTAAAGTC
 481 TATAAAAAGTCCTGAATTCTTAACAGACATAATATTATTCATACTGGAGAGAAACTCTACAAACCTGAAAGATGTGCCAA
 561 CGCTTTTGACAAGTCAAACTTTTCTAAACATCAAGAAAATCATGCTGCTGAGAAATCCTAGAAATGTGAAGAATGTGACA
 641 AAGCCTCTAAATGATTGTCACACTTGATCGTAGGTAAGATAATTCATACTGGAGAAAACTACCAGTGTGAACAATGTGGC
 721 CAAGCTTTGAACTAATGCTCACACCTTAATGCATAGGAAAGCATTTATGCTTGAGAAAAAGTGAACAAATATAGACAAAA
 801 AGCCATTAATACCTGCTCACATCGTACTCAAATTCAGAGCGTTCATACTAAATAAGAAGTGTAATTACTGTCAAACGACT
 881 GATCAGAAAATATGTCTTTAAAGTACAGAAGAGTATTTTGAAAAAGCATTACAAATATGAAGAGGCATTATTTATGACCT
 961 TTTCTATGGAAAGGTAAGGACATTAAAAATGTAAGATGCATGATGAAAATTTAAGTAGAGAGGATCTTTATGGTTAACTT
1041 ATAATATCGAGTGATGCATGAGGTAGATGTTCAGATTAATATTCTGCATTATTATGAAAGAAAAACATTCTTAATTTTAG
1121 TTAAAGTTTAGTATGATTTATTGTATTTTTATGAAATATATGTAGCATATTTTTAAATTATAAATTGCATGTGAACTTTT
1201 CAACCAAACAGTTTTAACATGTTAAATACTATCATGAATTCAATGAAGTGTTATTATGCCAGTAACTTTAACCTATTCCA
1281 CCTTACTCAAGGGTGTAGGTAAAAGATGGTAAAAATACACTATTTGGTAAGATAATGGACTGACATCTCTAGTGATTTTT
1361 TTTGCCAGTGAGTTTTAATTGCCATTAAGTTAAAGAATATTGTTCCCATAGGCTAAATTTTTATTTTTATTTTCTCATTT
1441 AAATGTATTTGACTTAATTTTTGTGGATACCTAATATGTGTACATATTATGCCATATGTGGCATATTTTGATACGGACAC
1521 ACAATATATAACAATCGCATTAGACTAAATGCGGTCTCCATTACCTCTAGCATTTATTCTTTGTATTACAAATAATTCAA
1601 TTCTACAGTTTTAGTTATTTTTAAATGTACAATTAAATTGTTGTTGACTACAGGATTATTTTTATGGTCATAATAAAACT
1681 TATATACAAACATAAGTAAAATCCATAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' gaccccaccccugUCUCCGAa 5'
                       ||||||| 
Target 5' tcccagctatgcgAGAGGCTg 3'
44 - 64 140.00 -9.40
2
miRNA  3' gaccccacccCUGUCUCCGaa 5'
                    || ||||||  
Target 5' ttacaaatatGA-AGAGGCat 3'
929 - 948 122.00 -7.60
3
miRNA  3' gaccCCACCCCUG---UCUCCGaa 5'
              || | | ||   :|||||  
Target 5' gaatGGCGTGAACCCGGGAGGCgg 3'
73 - 96 113.00 -12.70
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30167118 36 COSMIC
COSN31581382 56 COSMIC
COSN30533508 87 COSMIC
COSN1213368 103 COSMIC
COSN31514586 141 COSMIC
COSN31527108 155 COSMIC
COSN20115670 163 COSMIC
COSN31553928 182 COSMIC
COSN22869969 222 COSMIC
COSN21189775 227 COSMIC
COSN13794471 257 COSMIC
COSN31598405 272 COSMIC
COSN31573185 284 COSMIC
COSN31487960 295 COSMIC
COSN30515464 308 COSMIC
COSN22049425 318 COSMIC
COSN30521545 342 COSMIC
COSN30493849 347 COSMIC
COSN31529149 348 COSMIC
COSN30185818 355 COSMIC
COSN30485183 380 COSMIC
COSN31542098 447 COSMIC
COSN30493622 448 COSMIC
COSN30539759 452 COSMIC
COSN31609383 485 COSMIC
COSN26264570 538 COSMIC
COSN30541863 577 COSMIC
COSN31559319 675 COSMIC
COSN31526717 705 COSMIC
COSN5850533 1081 COSMIC
COSN6552150 1123 COSMIC
COSN26911911 1205 COSMIC
COSN23479187 1340 COSMIC
COSN15037289 1451 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs766964707 3 dbSNP
rs377238175 4 dbSNP
rs1297048760 5 dbSNP
rs1315207240 5 dbSNP
rs773721704 7 dbSNP
rs769274115 10 dbSNP
rs1284557477 12 dbSNP
rs1127612 14 dbSNP
rs749727661 17 dbSNP
rs776051430 19 dbSNP
rs559032423 20 dbSNP
rs1006376072 23 dbSNP
rs576328885 24 dbSNP
rs1168387740 27 dbSNP
rs537967627 31 dbSNP
rs1422829345 32 dbSNP
rs537339545 35 dbSNP
rs112364077 36 dbSNP
rs1264924338 38 dbSNP
rs777707589 39 dbSNP
rs1414924250 42 dbSNP
rs1480727915 43 dbSNP
rs1197698836 44 dbSNP
rs1260773280 46 dbSNP
rs1055260306 52 dbSNP
rs529280791 54 dbSNP
rs937790801 55 dbSNP
rs752780454 56 dbSNP
rs1046075122 58 dbSNP
rs1445769163 60 dbSNP
rs560321473 66 dbSNP
rs1185749327 67 dbSNP
rs552161364 79 dbSNP
rs949176461 80 dbSNP
rs1046100471 86 dbSNP
rs527938856 87 dbSNP
rs567097894 88 dbSNP
rs1336173237 90 dbSNP
rs1488609662 94 dbSNP
rs989293833 95 dbSNP
rs1285695269 98 dbSNP
rs1370962371 102 dbSNP
rs925108380 104 dbSNP
rs556172275 105 dbSNP
rs1307336302 106 dbSNP
rs910399566 111 dbSNP
rs540642276 112 dbSNP
rs1261978581 115 dbSNP
rs530264735 119 dbSNP
rs954817256 120 dbSNP
rs1480537167 125 dbSNP
rs1030742322 128 dbSNP
rs972070949 129 dbSNP
rs1177979996 131 dbSNP
rs1293542856 133 dbSNP
rs962253715 134 dbSNP
rs867158095 136 dbSNP
rs913251689 138 dbSNP
rs977189213 139 dbSNP
rs965389521 140 dbSNP
rs150388867 141 dbSNP
rs1368614413 142 dbSNP
rs1006969939 144 dbSNP
rs1441296444 146 dbSNP
rs1298537906 148 dbSNP
rs544290560 149 dbSNP
rs894609060 150 dbSNP
rs1422956091 154 dbSNP
rs532254646 157 dbSNP
rs1034534374 158 dbSNP
rs564823066 160 dbSNP
rs78216832 162 dbSNP
rs1205598386 163 dbSNP
rs78466301 163 dbSNP
rs1489910174 174 dbSNP
rs1199024799 177 dbSNP
rs1269670492 177 dbSNP
rs1417219782 177 dbSNP
rs1481493634 177 dbSNP
rs71163490 177 dbSNP
rs1409315921 181 dbSNP
rs1170819620 182 dbSNP
rs1027117082 185 dbSNP
rs906322607 187 dbSNP
rs1302198549 190 dbSNP
rs994441313 199 dbSNP
rs1438065565 201 dbSNP
rs116827273 210 dbSNP
rs1339253492 211 dbSNP
rs1215704582 214 dbSNP
rs1277178673 215 dbSNP
rs1357805191 222 dbSNP
rs1046668453 224 dbSNP
rs1200398475 234 dbSNP
rs371348033 235 dbSNP
rs573085260 236 dbSNP
rs1191041862 247 dbSNP
rs1241618633 249 dbSNP
rs1475018896 250 dbSNP
rs894808136 251 dbSNP
rs1391758433 254 dbSNP
rs554604466 256 dbSNP
rs533234475 257 dbSNP
rs1162527128 259 dbSNP
rs1365737162 260 dbSNP
rs1406764366 260 dbSNP
rs1315567305 262 dbSNP
rs936397837 267 dbSNP
rs1432086738 268 dbSNP
rs1300522805 270 dbSNP
rs542787413 271 dbSNP
rs575071114 275 dbSNP
rs1381035712 278 dbSNP
rs1245617366 284 dbSNP
rs556749990 288 dbSNP
rs1194957295 295 dbSNP
rs752984386 297 dbSNP
rs925118221 298 dbSNP
rs1283610350 300 dbSNP
rs537905687 301 dbSNP
rs945307268 318 dbSNP
rs570613243 321 dbSNP
rs1249890321 331 dbSNP
rs986018248 337 dbSNP
rs1182702384 344 dbSNP
rs1368869967 345 dbSNP
rs558906615 352 dbSNP
rs1473558800 353 dbSNP
rs533978001 366 dbSNP
rs923384988 367 dbSNP
rs570354845 369 dbSNP
rs1321978918 374 dbSNP
rs567137385 377 dbSNP
rs548628925 379 dbSNP
rs961996055 380 dbSNP
rs1016234901 387 dbSNP
rs759732126 401 dbSNP
rs1287993142 403 dbSNP
rs530445093 405 dbSNP
rs184008634 408 dbSNP
rs1391134864 418 dbSNP
rs543301935 419 dbSNP
rs1351486621 421 dbSNP
rs1027169995 422 dbSNP
rs958990760 425 dbSNP
rs1459896293 428 dbSNP
rs994366092 430 dbSNP
rs1256571100 432 dbSNP
rs1466986523 434 dbSNP
rs1179221144 440 dbSNP
rs961518231 443 dbSNP
rs1034928791 479 dbSNP
rs296094 482 dbSNP
rs906262806 490 dbSNP
rs1469709116 495 dbSNP
rs1024706141 498 dbSNP
rs1338012441 500 dbSNP
rs1013828607 505 dbSNP
rs1390090751 509 dbSNP
rs1328602228 512 dbSNP
rs1336514923 520 dbSNP
rs767931822 520 dbSNP
rs191208041 521 dbSNP
rs1322985694 526 dbSNP
rs185902520 532 dbSNP
rs1053451739 533 dbSNP
rs3745110 534 dbSNP
rs1249583603 536 dbSNP
rs6511433 537 dbSNP
rs1050671860 557 dbSNP
rs945251913 560 dbSNP
rs145988188 561 dbSNP
rs923128143 562 dbSNP
rs1280321173 563 dbSNP
rs1238564119 568 dbSNP
rs1167649724 572 dbSNP
rs901679644 574 dbSNP
rs1040703539 576 dbSNP
rs1301591105 577 dbSNP
rs543826846 583 dbSNP
rs972095289 584 dbSNP
rs542519554 585 dbSNP
rs940789230 593 dbSNP
rs763563169 595 dbSNP
rs984849062 602 dbSNP
rs1332708762 607 dbSNP
rs575182677 617 dbSNP
rs563093507 626 dbSNP
rs544849374 632 dbSNP
rs1225194503 633 dbSNP
rs774700760 638 dbSNP
rs985451024 639 dbSNP
rs1342670748 641 dbSNP
rs959205566 645 dbSNP
rs1289227829 650 dbSNP
rs1453990774 656 dbSNP
rs576915509 661 dbSNP
rs558817294 669 dbSNP
rs920030614 670 dbSNP
rs1193160780 674 dbSNP
rs534175239 678 dbSNP
rs972809031 679 dbSNP
rs1432090595 684 dbSNP
rs982000365 685 dbSNP
rs1025106964 688 dbSNP
rs1458627034 689 dbSNP
rs1458320579 690 dbSNP
rs1412018845 695 dbSNP
rs971525734 699 dbSNP
rs1468267965 704 dbSNP
rs1296859466 705 dbSNP
rs373754459 714 dbSNP
rs3745111 720 dbSNP
rs544757982 721 dbSNP
rs763287990 724 dbSNP
rs295368 733 dbSNP
rs1443951418 735 dbSNP
rs1241147767 738 dbSNP
rs1201889740 742 dbSNP
rs1221886452 743 dbSNP
rs1466342791 745 dbSNP
rs1484641883 755 dbSNP
rs1205060584 756 dbSNP
rs536900096 757 dbSNP
rs1451895811 779 dbSNP
rs1000514582 781 dbSNP
rs1385041802 784 dbSNP
rs1032000751 786 dbSNP
rs747735302 791 dbSNP
rs1167231242 796 dbSNP
rs1343494200 802 dbSNP
rs1453230491 802 dbSNP
rs903527858 802 dbSNP
rs1389207784 810 dbSNP
rs889011051 818 dbSNP
rs780940195 821 dbSNP
rs997856701 823 dbSNP
rs183063688 824 dbSNP
rs1314520810 829 dbSNP
rs40561 833 dbSNP
rs1237636265 837 dbSNP
rs539272920 840 dbSNP
rs571300983 841 dbSNP
rs909334842 844 dbSNP
rs1313602664 847 dbSNP
rs889069448 848 dbSNP
rs1049434541 852 dbSNP
rs1183812846 856 dbSNP
rs1049520460 858 dbSNP
rs937383320 859 dbSNP
rs1159935981 860 dbSNP
rs546352612 863 dbSNP
rs1345326147 864 dbSNP
rs1174264667 873 dbSNP
rs981682494 876 dbSNP
rs971641801 877 dbSNP
rs1304389414 881 dbSNP
rs1392364488 884 dbSNP
rs542587513 886 dbSNP
rs1288397744 893 dbSNP
rs1330667089 906 dbSNP
rs1443743027 907 dbSNP
rs1383121709 916 dbSNP
rs1222207057 920 dbSNP
rs1190488954 924 dbSNP
rs190105804 926 dbSNP
rs186651061 927 dbSNP
rs956427891 933 dbSNP
rs867560144 943 dbSNP
rs1032114104 946 dbSNP
rs1192488852 953 dbSNP
rs1231546987 955 dbSNP
rs1001039727 956 dbSNP
rs918095650 957 dbSNP
rs1445517905 958 dbSNP
rs1254056405 959 dbSNP
rs1479537249 963 dbSNP
rs992309511 966 dbSNP
rs181387084 970 dbSNP
rs1359232502 978 dbSNP
rs1033976646 979 dbSNP
rs1029085064 993 dbSNP
rs1177918648 998 dbSNP
rs997406303 1000 dbSNP
rs901809209 1001 dbSNP
rs1036146522 1002 dbSNP
rs189420638 1007 dbSNP
rs979647849 1014 dbSNP
rs533800480 1020 dbSNP
rs1402479796 1024 dbSNP
rs967698068 1037 dbSNP
rs1368853437 1038 dbSNP
rs1020540116 1039 dbSNP
rs887845858 1042 dbSNP
rs1269097127 1047 dbSNP
rs1009290539 1048 dbSNP
rs1427985568 1049 dbSNP
rs1049122011 1052 dbSNP
rs1489925998 1067 dbSNP
rs937429694 1071 dbSNP
rs905823816 1074 dbSNP
rs563282102 1081 dbSNP
rs372490644 1096 dbSNP
rs529594323 1096 dbSNP
rs1478297889 1102 dbSNP
rs1192902594 1106 dbSNP
rs1417114546 1110 dbSNP
rs1423582525 1114 dbSNP
rs1168992567 1118 dbSNP
rs1371960628 1120 dbSNP
rs1363549440 1124 dbSNP
rs1170249802 1134 dbSNP
rs1350000534 1143 dbSNP
rs186144057 1146 dbSNP
rs577443087 1153 dbSNP
rs1418642733 1180 dbSNP
rs1269004090 1181 dbSNP
rs142143561 1188 dbSNP
rs1229527283 1203 dbSNP
rs159356 1205 dbSNP
rs1049040341 1207 dbSNP
rs1357502283 1210 dbSNP
rs1290657776 1211 dbSNP
rs994722813 1211 dbSNP
rs1453460286 1212 dbSNP
rs1223666210 1214 dbSNP
rs767841294 1219 dbSNP
rs1271113027 1223 dbSNP
rs1448805308 1243 dbSNP
rs573291193 1245 dbSNP
rs1200489235 1254 dbSNP
rs1490245723 1257 dbSNP
rs1267214988 1260 dbSNP
rs1391811491 1261 dbSNP
rs1207673608 1265 dbSNP
rs1162330190 1266 dbSNP
rs1413866721 1291 dbSNP
rs554663456 1298 dbSNP
rs1406538599 1301 dbSNP
rs936129330 1302 dbSNP
rs1308626083 1304 dbSNP
rs200829637 1305 dbSNP
rs560918286 1306 dbSNP
rs1388491288 1307 dbSNP
rs926129075 1308 dbSNP
rs940110782 1311 dbSNP
rs918036388 1316 dbSNP
rs1244319604 1318 dbSNP
rs1056931778 1320 dbSNP
rs938228155 1326 dbSNP
rs1358741008 1335 dbSNP
rs1272877016 1336 dbSNP
rs1213295284 1346 dbSNP
rs926751700 1350 dbSNP
rs538438571 1351 dbSNP
rs1283353275 1352 dbSNP
rs1209317967 1362 dbSNP
rs913584022 1364 dbSNP
rs968250627 1364 dbSNP
rs1404024851 1366 dbSNP
rs1473589801 1367 dbSNP
rs575995379 1372 dbSNP
rs557716286 1373 dbSNP
rs1442611383 1383 dbSNP
rs1458210450 1388 dbSNP
rs1028657560 1389 dbSNP
rs975755680 1390 dbSNP
rs539365060 1393 dbSNP
rs965921078 1398 dbSNP
rs1395739481 1399 dbSNP
rs571712777 1402 dbSNP
rs1342437548 1411 dbSNP
rs1440331736 1426 dbSNP
rs1416085952 1428 dbSNP
rs1287766811 1444 dbSNP
rs751626952 1455 dbSNP
rs547184778 1471 dbSNP
rs1027450522 1473 dbSNP
rs887583920 1478 dbSNP
rs1177155558 1482 dbSNP
rs1027903631 1484 dbSNP
rs112482832 1485 dbSNP
rs897714921 1486 dbSNP
rs1250408637 1491 dbSNP
rs1472672044 1494 dbSNP
rs1036265531 1495 dbSNP
rs1427405383 1497 dbSNP
rs1465998526 1500 dbSNP
rs534377067 1507 dbSNP
rs567403681 1514 dbSNP
rs896472711 1515 dbSNP
rs1232645074 1524 dbSNP
rs1351175949 1524 dbSNP
rs766600861 1528 dbSNP
rs1281484147 1531 dbSNP
rs29991 1533 dbSNP
rs182212335 1537 dbSNP
rs1043845788 1538 dbSNP
rs569594142 1539 dbSNP
rs551234172 1552 dbSNP
rs533073596 1553 dbSNP
rs965772625 1565 dbSNP
rs1289876301 1569 dbSNP
rs1206617660 1570 dbSNP
rs559236233 1572 dbSNP
rs891818457 1573 dbSNP
rs1433078023 1579 dbSNP
rs911512310 1586 dbSNP
rs1379718885 1592 dbSNP
rs541266920 1594 dbSNP
rs1450130877 1597 dbSNP
rs1053334811 1600 dbSNP
rs953224500 1601 dbSNP
rs1431896251 1604 dbSNP
rs936247009 1605 dbSNP
rs1337492104 1607 dbSNP
rs1027499785 1614 dbSNP
rs1301981769 1617 dbSNP
rs994699895 1618 dbSNP
rs757176000 1631 dbSNP
rs528567045 1643 dbSNP
rs1014887657 1647 dbSNP
rs1014555537 1649 dbSNP
rs986073940 1653 dbSNP
rs549638003 1672 dbSNP
rs931744357 1680 dbSNP
rs561466675 1683 dbSNP
rs1035411284 1684 dbSNP
rs1175779313 1692 dbSNP
rs1270010689 1705 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084040. RNA binding protein: AGO2. Condition:CLIP_noarsenite_rep1 HITS-CLIP data was present in GSM1084041. RNA binding protein: AGO2. Condition:CLIP_arsenite_rep1 HITS-CLIP data was present in GSM1084042. RNA binding protein: AGO2. Condition:CLIP_noarsenite_rep2 HITS-CLIP data was present in GSM1084065. RNA binding protein: AGO2. Condition:CLIP_emetine_AbnovaAb HITS-CLIP data was present in GSM1084072. RNA binding protein: AGO2. Condition:CLIP_nohippuristanol_rep1_AbnovaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Conditions Prostate Tissue
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... PAR-CLIP data was present in SRX1760616. RNA binding protein: AGO2. Condition:AGO-CLIP-PC3_A PAR-CLIP data was present in SRX1760630. RNA binding protein: AGO2. Condition:AGO-CLIP-22RV1_A ...

- Hamilton MP; Rajapakshe KI; Bader DA; Cerne et al., 2016, Neoplasia (New York, N.Y.).

Article - Hamilton MP; Rajapakshe KI; Bader DA; Cerne et al.
- Neoplasia (New York, N.Y.), 2016
MicroRNA (miRNA) deregulation in prostate cancer (PCa) contributes to PCa initiation and metastatic progression. To comprehensively define the cancer-associated changes in miRNA targeting and function in commonly studied models of PCa, we performed photoactivatable ribonucleoside-enhanced cross-linking immunoprecipitation of the Argonaute protein in a panel of PCa cell lines modeling different stages of PCa progression. Using this comprehensive catalogue of miRNA targets, we analyzed miRNA targeting on known drivers of PCa and examined tissue-specific and stage-specific pathway targeting by miRNAs. We found that androgen receptor is the most frequently targeted PCa oncogene and that miR-148a targets the largest number of known PCa drivers. Globally, tissue-specific and stage-specific changes in miRNA targeting are driven by homeostatic response to active oncogenic pathways. Our findings indicate that, even in advanced PCa, the miRNA pool adapts to regulate continuing alterations in the cancer genome to balance oncogenic molecular changes. These findings are important because they are the first to globally characterize miRNA changes in PCa and demonstrate how the miRNA target spectrum responds to staged tumorigenesis.
LinkOut: [PMID: 27292025]
CLIP-seq Support 1 for dataset GSM1084040
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_noarsenite_rep1
Location of target site ENST00000300619.7 | 3UTR | GCGAGAGGCUGAGGCAGG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM1084041
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_arsenite_rep1
Location of target site ENST00000300619.7 | 3UTR | AGAGGCUGAGGCAGGAGAAUGGCGUGAACCCGGGAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM1084042
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_noarsenite_rep2
Location of target site ENST00000300619.7 | 3UTR | AUGCGAGAGGCUGAGGCAGGAGAAUGGCGUGAACCC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM1084065
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_emetine_AbnovaAb
Location of target site ENST00000300619.7 | 3UTR | AUGCGAGAGGCUGAGGCAGGAGAAUGGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 5 for dataset GSM1084072
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_nohippuristanol_rep1_AbnovaAb
Location of target site ENST00000300619.7 | 3UTR | AUGCGAGAGGCUGAGGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
108 hsa-miR-6819-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT358318 PANK3 pantothenate kinase 3 2 2
MIRT454719 UBQLNL ubiquilin like 2 2
MIRT471362 PEG10 paternally expressed 10 2 2
MIRT478101 DLG5 discs large MAGUK scaffold protein 5 2 4
MIRT495554 GOLGA6L4 golgin A6 family-like 4 2 2
MIRT495587 GOLGA6L10 golgin A6 family-like 10 2 2
MIRT508315 RPL18 ribosomal protein L18 2 4
MIRT529183 ADAM33 ADAM metallopeptidase domain 33 2 2
MIRT532035 FHDC1 FH2 domain containing 1 2 2
MIRT545131 ANXA5 annexin A5 2 2
MIRT567288 HNRNPL heterogeneous nuclear ribonucleoprotein L 2 2
MIRT568094 CEBPG CCAAT/enhancer binding protein gamma 2 2
MIRT573291 DLX4 distal-less homeobox 4 2 2
MIRT575169 Fam120c family with sequence similarity 120, member C 2 2
MIRT609495 DNASE1L3 deoxyribonuclease 1 like 3 2 2
MIRT611264 EHD3 EH domain containing 3 2 2
MIRT613580 POC5 POC5 centriolar protein 2 2
MIRT616520 C9orf170 chromosome 9 open reading frame 170 2 2
MIRT617098 ZNF667 zinc finger protein 667 2 2
MIRT618268 DDX51 DEAD-box helicase 51 2 2
MIRT618294 ZNF682 zinc finger protein 682 2 2
MIRT618617 SHOX short stature homeobox 2 2
MIRT619741 SRFBP1 serum response factor binding protein 1 2 2
MIRT619784 NRIP2 nuclear receptor interacting protein 2 2 2
MIRT621119 SP110 SP110 nuclear body protein 2 2
MIRT622530 RAD51 RAD51 recombinase 2 2
MIRT623088 NME6 NME/NM23 nucleoside diphosphate kinase 6 2 2
MIRT623938 FKBP14 FK506 binding protein 14 2 2
MIRT624389 CD84 CD84 molecule 2 2
MIRT624860 ABI2 abl interactor 2 2 2
MIRT628752 TRPV2 transient receptor potential cation channel subfamily V member 2 2 2
MIRT632243 VTA1 vesicle trafficking 1 2 2
MIRT632987 E2F2 E2F transcription factor 2 2 2
MIRT633695 RBM43 RNA binding motif protein 43 2 2
MIRT633766 CENPBD1 CENPB DNA-binding domain containing 1 2 2
MIRT633808 ZNF91 zinc finger protein 91 2 2
MIRT638914 CBLN2 cerebellin 2 precursor 2 2
MIRT641298 SLAMF1 signaling lymphocytic activation molecule family member 1 2 2
MIRT642123 SRCAP Snf2 related CREBBP activator protein 2 2
MIRT642179 HEBP2 heme binding protein 2 2 2
MIRT642617 CDKN3 cyclin dependent kinase inhibitor 3 2 2
MIRT643325 TMEM151B transmembrane protein 151B 2 2
MIRT644534 TMEM134 transmembrane protein 134 2 2
MIRT645820 OMA1 OMA1 zinc metallopeptidase 2 2
MIRT646093 MGST3 microsomal glutathione S-transferase 3 2 2
MIRT646861 SLC35E4 solute carrier family 35 member E4 2 2
MIRT647466 DZIP1L DAZ interacting zinc finger protein 1 like 2 2
MIRT647702 NADSYN1 NAD synthetase 1 2 2
MIRT648082 ZMIZ2 zinc finger MIZ-type containing 2 2 2
MIRT648547 TMEM169 transmembrane protein 169 2 2
MIRT648690 AP1M1 adaptor related protein complex 1 mu 1 subunit 2 2
MIRT649747 GNB5 G protein subunit beta 5 2 2
MIRT650154 ZNF426 zinc finger protein 426 2 2
MIRT650450 CPXM2 carboxypeptidase X, M14 family member 2 2 2
MIRT650943 CTNS cystinosin, lysosomal cystine transporter 2 2
MIRT651544 WNK1 WNK lysine deficient protein kinase 1 2 2
MIRT652304 TNRC6B trinucleotide repeat containing 6B 2 2
MIRT652890 SYVN1 synoviolin 1 2 2
MIRT654222 RNF19B ring finger protein 19B 2 2
MIRT654676 PSMB5 proteasome subunit beta 5 2 2
MIRT658249 FAXC failed axon connections homolog 2 2
MIRT658363 FAM65B RHO family interacting cell polarization regulator 2 2 2
MIRT659622 CELF1 CUGBP Elav-like family member 1 2 2
MIRT660171 BNIP3L BCL2 interacting protein 3 like 2 2
MIRT660634 ANKRD52 ankyrin repeat domain 52 2 2
MIRT662428 TMCO1 transmembrane and coiled-coil domains 1 2 2
MIRT662984 PAK3 p21 (RAC1) activated kinase 3 2 2
MIRT663939 ZNF554 zinc finger protein 554 2 2
MIRT666960 PKHD1 PKHD1, fibrocystin/polyductin 2 2
MIRT667945 HNRNPC heterogeneous nuclear ribonucleoprotein C (C1/C2) 2 2
MIRT668847 CYCS cytochrome c, somatic 2 2
MIRT671792 RGS17 regulator of G protein signaling 17 2 2
MIRT672382 RPL37 ribosomal protein L37 2 2
MIRT673516 TNFAIP8L1 TNF alpha induced protein 8 like 1 2 4
MIRT674716 FAM73A mitoguardin 1 2 2
MIRT676352 KLF8 Kruppel like factor 8 2 2
MIRT677820 MRPS10 mitochondrial ribosomal protein S10 2 2
MIRT678440 PDE4C phosphodiesterase 4C 2 2
MIRT678499 ARHGEF39 Rho guanine nucleotide exchange factor 39 2 2
MIRT679243 LRP10 LDL receptor related protein 10 2 2
MIRT693607 SLC39A1 solute carrier family 39 member 1 2 2
MIRT695927 ZNF174 zinc finger protein 174 2 2
MIRT697987 TSPAN6 tetraspanin 6 2 2
MIRT700821 PHLDA2 pleckstrin homology like domain family A member 2 2 2
MIRT701350 NR4A3 nuclear receptor subfamily 4 group A member 3 2 2
MIRT702348 KLHL7 kelch like family member 7 2 2
MIRT703387 GABPB1 GA binding protein transcription factor beta subunit 1 2 2
MIRT703883 ERBB2IP erbb2 interacting protein 2 2
MIRT708237 PPP1R26 protein phosphatase 1 regulatory subunit 26 2 2
MIRT708629 NUDT18 nudix hydrolase 18 2 2
MIRT708990 CABP4 calcium binding protein 4 2 2
MIRT709804 FOXE1 forkhead box E1 2 2
MIRT710203 FLVCR1 feline leukemia virus subgroup C cellular receptor 1 2 2
MIRT710248 VAMP1 vesicle associated membrane protein 1 2 2
MIRT710281 FGF1 fibroblast growth factor 1 2 2
MIRT712992 IRGQ immunity related GTPase Q 2 2
MIRT713887 MOB3A MOB kinase activator 3A 2 2
MIRT715600 ADAM17 ADAM metallopeptidase domain 17 2 2
MIRT715639 ZBTB8B zinc finger and BTB domain containing 8B 2 2
MIRT715686 FRK fyn related Src family tyrosine kinase 2 2
MIRT717053 WIZ widely interspaced zinc finger motifs 2 2
MIRT717611 DSTYK dual serine/threonine and tyrosine protein kinase 2 2
MIRT721539 DKK3 dickkopf WNT signaling pathway inhibitor 3 2 2
MIRT722096 SUSD1 sushi domain containing 1 2 2
MIRT722435 HRNR hornerin 2 2
MIRT722855 NEGR1 neuronal growth regulator 1 2 2
MIRT724621 SNAP25 synaptosome associated protein 25 2 2
MIRT724777 PSG4 pregnancy specific beta-1-glycoprotein 4 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-6819 Ceritinib 57379345 NSC776422 approved resistant High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-miR-6819-3p Ceritinib 57379345 NSC776422 approved resistant High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-miR-6819-3p Cisplatin 5460033 NSC119875 approved resistant High Hypopharyngeal Cancer cell line (FaDu)
hsa-miR-6819-3p Gefitinib 123631 NSC715055 approved sensitive cell line (PC9)
hsa-miR-6819-3p Osimertinib 71496458 NSC779217 approved resistant cell line (HCC827)
hsa-miR-6819-3p Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-6819-3p Osimertinib 71496458 NSC779217 approved resistant cell line (H1975)
hsa-miR-6819-3p Cisplatin 5460033 NSC119875 approved resistant cell line (A2780)

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