ID	Duplex structure	Position	Score	MFE
1	miRNA 3' gacaggaACCGU---UUCUCUUgu 5' || || :|||||| Target 5' taattgaTGACATTTGAGAGAAat 3'	1000 - 1023	126.00	-5.90
2	miRNA 3' gaCAGGAACCGU----UUCUCUUGu 5' || :|| | | |||| ||| Target 5' aaGTGTTTAGGATTTCAAGACAACa 3'	340 - 364	121.00	-8.40
3	miRNA 3' gaCAGGAACCG--UUUCUCUUgu 5' || :|| : ||:||||| Target 5' aaGTATTTTATAAAAGGAGAAag 3'	243 - 265	117.00	-6.60

Mutant ID	Mutant Position	Mutant Source
COSM240903	1	COSMIC
COSN31502248	7	COSMIC
COSN30497304	13	COSMIC
COSN5076842	26	COSMIC
COSN30535149	68	COSMIC
COSN31509711	70	COSMIC
COSN30191290	88	COSMIC
COSN31502465	167	COSMIC
COSN20101203	190	COSMIC
COSN20101204	435	COSMIC
COSN20566433	506	COSMIC
COSN15734450	559	COSMIC
COSN29618111	618	COSMIC
COSN8631079	1004	COSMIC
COSN14616786	1091	COSMIC
COSN15343447	1286	COSMIC
COSN23106773	1387	COSMIC
COSN25088279	1428	COSMIC
COSN9447607	1527	COSMIC
COSN23228947	1715	COSMIC
COSN27995977	1988	COSMIC
COSN27304089	2074	COSMIC
COSN16501844	2176	COSMIC
COSN17226150	2269	COSMIC
COSN7942993	2331	COSMIC
COSN19552123	2356	COSMIC
COSN28505059	2483	COSMIC
COSN27755851	2848	COSMIC
COSN2107110	2893	COSMIC

Mutant ID	Mutant Position	Mutant Source
rs922340043	5	dbSNP
rs1278045307	10	dbSNP
rs945738367	16	dbSNP
rs1216350199	17	dbSNP
rs750730374	19	dbSNP
rs756238212	23	dbSNP
rs191475277	28	dbSNP
rs754329623	30	dbSNP
rs1269410935	31	dbSNP
rs1163048024	35	dbSNP
rs755463308	36	dbSNP
rs55984047	37	dbSNP
rs925578528	43	dbSNP
rs16873502	51	dbSNP
rs1056099615	56	dbSNP
rs147932866	58	dbSNP
rs1225304813	60	dbSNP
rs1357969918	63	dbSNP
rs1288920472	64	dbSNP
rs1244671407	66	dbSNP
rs1317128931	69	dbSNP
rs894431719	71	dbSNP
rs1221722203	76	dbSNP
rs534489214	78	dbSNP
rs1476458045	82	dbSNP
rs1054833510	84	dbSNP
rs1363686799	85	dbSNP
rs184272260	86	dbSNP
rs948794691	89	dbSNP
rs897667100	92	dbSNP
rs1157116177	93	dbSNP
rs1008185384	104	dbSNP
rs1418742046	117	dbSNP
rs1044522730	136	dbSNP
rs1339122870	142	dbSNP
rs907307577	145	dbSNP
rs1019612724	148	dbSNP
rs1268874974	148	dbSNP
rs1333144780	149	dbSNP
rs1027753514	155	dbSNP
rs1261990766	156	dbSNP
rs371409962	161	dbSNP
rs777029045	165	dbSNP
rs538347397	166	dbSNP
rs1283657135	167	dbSNP
rs1225174067	179	dbSNP
rs34053391	184	dbSNP
rs1056248207	193	dbSNP
rs886998545	195	dbSNP
rs558187103	209	dbSNP
rs187591100	217	dbSNP
rs113832882	221	dbSNP
rs1407177291	223	dbSNP
rs34304579	223	dbSNP
rs1016993800	230	dbSNP
rs962447762	232	dbSNP
rs996992904	234	dbSNP
rs1021109684	236	dbSNP
rs1028038942	239	dbSNP
rs1430834954	241	dbSNP
rs1262759896	246	dbSNP
rs1202902062	256	dbSNP
rs1464148520	258	dbSNP
rs1448813717	260	dbSNP
rs955110685	263	dbSNP
rs1191606833	265	dbSNP
rs1380005595	269	dbSNP
rs1321078740	273	dbSNP
rs1263031790	276	dbSNP
rs1228013244	280	dbSNP
rs1328171801	283	dbSNP
rs1293496408	284	dbSNP
rs986942421	294	dbSNP
rs1383794136	295	dbSNP
rs1338872730	297	dbSNP
rs924785805	305	dbSNP
rs1405752193	311	dbSNP
rs1178986143	315	dbSNP
rs1168889254	323	dbSNP
rs1376253083	332	dbSNP
rs977154098	338	dbSNP
rs925746350	339	dbSNP
rs745989161	341	dbSNP
rs956261921	343	dbSNP
rs1448279492	356	dbSNP
rs935663440	361	dbSNP
rs991204921	363	dbSNP
rs1184998443	369	dbSNP
rs1171627324	373	dbSNP
rs1255177142	376	dbSNP
rs1196210174	390	dbSNP
rs1341372225	396	dbSNP
rs915568418	410	dbSNP
rs73753606	411	dbSNP
rs914552447	420	dbSNP
rs1045642830	425	dbSNP
rs138980708	425	dbSNP
rs796540619	425	dbSNP
rs1340145074	426	dbSNP
rs111607585	427	dbSNP
rs770327974	428	dbSNP
rs1394785480	435	dbSNP
rs1339708449	441	dbSNP
rs1167783098	442	dbSNP
rs1425868852	458	dbSNP
rs1405447817	460	dbSNP
rs1385269456	462	dbSNP
rs796462613	464	dbSNP
rs574685772	465	dbSNP
rs540520330	469	dbSNP
rs1007966393	497	dbSNP
rs141702531	498	dbSNP
rs184650537	500	dbSNP
rs886967328	523	dbSNP
rs1294008090	528	dbSNP
rs1243941582	529	dbSNP
rs1355900594	537	dbSNP
rs72760484	538	dbSNP
rs1285441269	557	dbSNP
rs1241463430	577	dbSNP
rs546335560	583	dbSNP
rs1334299334	586	dbSNP
rs1038325107	591	dbSNP
rs368502379	596	dbSNP
rs1368059165	600	dbSNP
rs1304417628	601	dbSNP
rs1403165755	605	dbSNP
rs1412452390	612	dbSNP
rs1021243967	621	dbSNP
rs1470973113	623	dbSNP
rs1361811667	629	dbSNP
rs1181241049	637	dbSNP
rs1265953367	640	dbSNP
rs1480712790	650	dbSNP
rs1271042677	651	dbSNP
rs143831033	652	dbSNP
rs1183001907	656	dbSNP
rs1220162371	669	dbSNP
rs372923830	671	dbSNP
rs1451047493	675	dbSNP
rs1363967283	680	dbSNP
rs751693152	687	dbSNP
rs547287174	688	dbSNP
rs1348861254	690	dbSNP
rs996486777	698	dbSNP
rs1224571947	699	dbSNP
rs966846776	702	dbSNP
rs531818374	711	dbSNP
rs1281860091	716	dbSNP
rs1436410357	725	dbSNP
rs1359324110	731	dbSNP
rs1001072858	732	dbSNP
rs1402058516	734	dbSNP
rs1424805920	739	dbSNP
rs1028424046	743	dbSNP
rs1480600537	744	dbSNP
rs1365415851	755	dbSNP
rs190318810	758	dbSNP
rs116409496	766	dbSNP
rs971052559	776	dbSNP
rs768906780	788	dbSNP
rs1238208509	794	dbSNP
rs1204550443	795	dbSNP
rs562571421	797	dbSNP
rs181406943	803	dbSNP
rs1360241798	804	dbSNP
rs1223955674	805	dbSNP
rs1264142172	808	dbSNP
rs1488452961	814	dbSNP
rs1031824317	821	dbSNP
rs1363326284	836	dbSNP
rs1270251246	838	dbSNP
rs151107527	840	dbSNP
rs760421909	845	dbSNP
rs1196706064	851	dbSNP
rs1432359179	857	dbSNP
rs990798324	862	dbSNP
rs1240379748	877	dbSNP
rs1319398540	882	dbSNP
rs377326235	889	dbSNP
rs566138928	890	dbSNP
rs1021840802	897	dbSNP
rs970737217	925	dbSNP
rs1176377341	937	dbSNP
rs1379369038	940	dbSNP
rs1048923167	942	dbSNP
rs980099074	949	dbSNP
rs1169721064	950	dbSNP
rs1447663487	952	dbSNP
rs928669823	956	dbSNP
rs943430028	957	dbSNP
rs1241655396	963	dbSNP
rs202207795	963	dbSNP
rs1209921383	965	dbSNP
rs1308591549	966	dbSNP
rs768190254	977	dbSNP
rs1457376191	978	dbSNP
rs1298721585	979	dbSNP
rs1452872956	998	dbSNP
rs1384604946	1000	dbSNP
rs184732363	1004	dbSNP
rs1444376366	1012	dbSNP
rs766180637	1014	dbSNP
rs189141866	1019	dbSNP
rs1172375071	1023	dbSNP
rs1308798601	1024	dbSNP
rs1383817689	1027	dbSNP
rs14491	1030	dbSNP
rs901830628	1030	dbSNP
rs983540716	1036	dbSNP
rs908375322	1037	dbSNP
rs1310700223	1038	dbSNP
rs1411975325	1042	dbSNP
rs750954766	1044	dbSNP
rs776246599	1046	dbSNP
rs997538497	1056	dbSNP
rs1038613433	1064	dbSNP
rs898389247	1065	dbSNP
rs1202983967	1067	dbSNP
rs1042657946	1073	dbSNP
rs932572547	1092	dbSNP
rs1049409430	1094	dbSNP
rs568648829	1096	dbSNP
rs537939919	1103	dbSNP
rs1228762258	1108	dbSNP
rs890817847	1119	dbSNP
rs1007976575	1120	dbSNP
rs1456711013	1122	dbSNP
rs1373729657	1125	dbSNP
rs1031456791	1126	dbSNP
rs1299699808	1129	dbSNP
rs1198411949	1130	dbSNP
rs1032471064	1139	dbSNP
rs891544513	1141	dbSNP
rs554516664	1148	dbSNP
rs1164280990	1149	dbSNP
rs1012640047	1150	dbSNP
rs1011724622	1160	dbSNP
rs1022699473	1174	dbSNP
rs1180529692	1180	dbSNP
rs113936896	1182	dbSNP
rs970323680	1188	dbSNP
rs981114928	1189	dbSNP
rs1489142251	1196	dbSNP
rs980785902	1197	dbSNP
rs112625662	1212	dbSNP
rs1483388425	1227	dbSNP
rs533854658	1230	dbSNP
rs1317187401	1231	dbSNP
rs1183058964	1233	dbSNP
rs1410955171	1243	dbSNP
rs1308855199	1245	dbSNP
rs950603747	1247	dbSNP
rs1349797312	1251	dbSNP
rs1283779586	1252	dbSNP
rs1422782262	1254	dbSNP
rs186242454	1258	dbSNP
rs985118071	1265	dbSNP
rs1171286929	1269	dbSNP
rs1035652394	1281	dbSNP
rs1425772541	1283	dbSNP
rs190725045	1284	dbSNP
rs546247932	1285	dbSNP
rs716960	1286	dbSNP
rs569254928	1306	dbSNP
rs1202164842	1313	dbSNP
rs1431979383	1327	dbSNP
rs974834692	1336	dbSNP
rs1264618564	1362	dbSNP
rs1485984751	1362	dbSNP
rs1269706789	1366	dbSNP
rs200317908	1366	dbSNP
rs1318719390	1368	dbSNP
rs1434676407	1368	dbSNP
rs181517819	1368	dbSNP
rs70997432	1368	dbSNP
rs1407251401	1370	dbSNP
rs138029232	1376	dbSNP
rs923387764	1377	dbSNP
rs1464966396	1384	dbSNP
rs1377618041	1385	dbSNP
rs1428527483	1387	dbSNP
rs963814101	1387	dbSNP
rs397811308	1388	dbSNP
rs973903171	1389	dbSNP
rs1448477549	1390	dbSNP
rs919782806	1398	dbSNP
rs1412012677	1400	dbSNP
rs796364031	1412	dbSNP
rs1042377469	1414	dbSNP
rs933299980	1414	dbSNP
rs1280570087	1420	dbSNP
rs1383736092	1430	dbSNP
rs1339116599	1436	dbSNP
rs902518088	1438	dbSNP
rs936914025	1440	dbSNP
rs1299299172	1444	dbSNP
rs1463955363	1458	dbSNP
rs1264179002	1460	dbSNP
rs1166122884	1469	dbSNP
rs576649903	1470	dbSNP
rs1385402134	1472	dbSNP
rs895304074	1479	dbSNP
rs1440545691	1490	dbSNP
rs1255189474	1492	dbSNP
rs1211773377	1494	dbSNP
rs1235439736	1501	dbSNP
rs186241226	1505	dbSNP
rs1022667070	1509	dbSNP
rs1227682875	1518	dbSNP
rs1360439986	1524	dbSNP
rs1286239060	1528	dbSNP
rs1242861548	1532	dbSNP
rs943650011	1539	dbSNP
rs10069201	1545	dbSNP
rs1308112638	1552	dbSNP
rs1476817864	1560	dbSNP
rs1369188982	1566	dbSNP
rs1002588339	1569	dbSNP
rs141085785	1573	dbSNP
rs1169923255	1575	dbSNP
rs1425578312	1583	dbSNP
rs1428623934	1588	dbSNP
rs891517855	1596	dbSNP
rs1025898200	1597	dbSNP
rs1471587929	1600	dbSNP
rs1011772262	1609	dbSNP
rs1043214033	1612	dbSNP
rs1174212131	1616	dbSNP
rs950455474	1629	dbSNP
rs150309124	1630	dbSNP
rs1473538862	1638	dbSNP
rs137878316	1640	dbSNP
rs1001735752	1652	dbSNP
rs964571277	1653	dbSNP
rs1490549805	1657	dbSNP
rs1339044076	1677	dbSNP
rs541374820	1683	dbSNP
rs556122133	1683	dbSNP
rs1035869548	1685	dbSNP
rs1292081334	1715	dbSNP
rs564408837	1716	dbSNP
rs1208323495	1722	dbSNP
rs1355850203	1730	dbSNP
rs975014237	1731	dbSNP
rs1230550186	1735	dbSNP
rs960284123	1744	dbSNP
rs533432865	1751	dbSNP
rs1288335224	1759	dbSNP
rs923106654	1769	dbSNP
rs933352457	1772	dbSNP
rs1370166778	1774	dbSNP
rs1014891447	1776	dbSNP
rs1324907943	1786	dbSNP
rs1403266789	1791	dbSNP
rs963480981	1808	dbSNP
rs1275985075	1815	dbSNP
rs1159634143	1825	dbSNP
rs978189000	1827	dbSNP
rs973918660	1828	dbSNP
rs1181423429	1840	dbSNP
rs1480702625	1844	dbSNP
rs34289650	1849	dbSNP
rs1196780007	1850	dbSNP
rs1451259815	1851	dbSNP
rs954046858	1861	dbSNP
rs142465518	1865	dbSNP
rs1287497877	1871	dbSNP
rs985296925	1873	dbSNP
rs531311946	1875	dbSNP
rs1487050199	1895	dbSNP
rs936697257	1897	dbSNP
rs912442275	1898	dbSNP
rs1267862181	1922	dbSNP
rs943951304	1932	dbSNP
rs190700755	1935	dbSNP
rs912282013	1935	dbSNP
rs948240691	1935	dbSNP
rs144632030	1936	dbSNP
rs1436796356	1944	dbSNP
rs1386229255	1954	dbSNP
rs1002557034	1970	dbSNP
rs1414988198	1971	dbSNP
rs1189260766	1973	dbSNP
rs1427100028	1973	dbSNP
rs1170739593	1977	dbSNP
rs1026047339	1979	dbSNP
rs1242755703	1989	dbSNP
rs35429460	1999	dbSNP
rs1463681109	2000	dbSNP
rs1420576389	2009	dbSNP
rs947156533	2012	dbSNP
rs554487506	2020	dbSNP
rs1277199884	2025	dbSNP
rs1219141254	2029	dbSNP
rs1043232773	2030	dbSNP
rs905620194	2037	dbSNP
rs182894790	2046	dbSNP
rs1230194820	2047	dbSNP
rs1362514884	2050	dbSNP
rs746565356	2051	dbSNP
rs574967807	2053	dbSNP
rs1359602026	2062	dbSNP
rs1448572761	2062	dbSNP
rs974660159	2062	dbSNP
rs1352130089	2070	dbSNP
rs62368580	2076	dbSNP
rs1030144815	2077	dbSNP
rs954540069	2098	dbSNP
rs1389710173	2109	dbSNP
rs1057248518	2123	dbSNP
rs1484474756	2127	dbSNP
rs1374556599	2129	dbSNP
rs896043195	2131	dbSNP
rs116460111	2133	dbSNP
rs1274755465	2175	dbSNP
rs1206599911	2179	dbSNP
rs1311072440	2179	dbSNP
rs138690778	2183	dbSNP
rs1014908013	2185	dbSNP
rs963746664	2186	dbSNP
rs539734075	2201	dbSNP
rs1381128156	2202	dbSNP
rs1282172726	2218	dbSNP
rs1447230018	2221	dbSNP
rs943790203	2222	dbSNP
rs1309941805	2223	dbSNP
rs989922099	2227	dbSNP
rs994922075	2231	dbSNP
rs1245884518	2232	dbSNP
rs535252358	2233	dbSNP
rs1029455819	2235	dbSNP
rs1165806997	2240	dbSNP
rs1180734847	2245	dbSNP
rs953442930	2248	dbSNP
rs1425746535	2250	dbSNP
rs985264260	2254	dbSNP
rs770323169	2255	dbSNP
rs991059468	2256	dbSNP
rs535618147	2260	dbSNP
rs930166943	2263	dbSNP
rs1047465813	2265	dbSNP
rs1481816115	2268	dbSNP
rs965272324	2269	dbSNP
rs988768892	2271	dbSNP
rs912890742	2281	dbSNP
rs947009588	2282	dbSNP
rs978453382	2289	dbSNP
rs1350878579	2290	dbSNP
rs886219341	2300	dbSNP
rs576338824	2303	dbSNP
rs1294144419	2311	dbSNP
rs1459658318	2317	dbSNP
rs1404062264	2318	dbSNP
rs927012138	2328	dbSNP
rs545501986	2331	dbSNP
rs879873788	2332	dbSNP
rs1323535305	2337	dbSNP
rs1458152625	2339	dbSNP
rs1414625543	2340	dbSNP
rs1057215333	2341	dbSNP
rs1180699720	2351	dbSNP
rs1472528876	2355	dbSNP
rs1454708141	2356	dbSNP
rs1249237427	2361	dbSNP
rs1401463483	2366	dbSNP
rs1191213890	2369	dbSNP
rs1489260099	2370	dbSNP
rs375050183	2371	dbSNP
rs1005974583	2379	dbSNP
rs896010658	2395	dbSNP
rs541965021	2402	dbSNP
rs1037053562	2405	dbSNP
rs1329667304	2406	dbSNP
rs149288188	2408	dbSNP
rs995205017	2425	dbSNP
rs1029011000	2428	dbSNP
rs1237642410	2438	dbSNP
rs1284307072	2449	dbSNP
rs1302084744	2452	dbSNP
rs1357900969	2454	dbSNP
rs889193512	2458	dbSNP
rs1226517628	2460	dbSNP
rs1293211687	2468	dbSNP
rs1436052473	2477	dbSNP
rs1391329588	2478	dbSNP
rs368384741	2483	dbSNP
rs1019458211	2484	dbSNP
rs1378651636	2486	dbSNP
rs1434456758	2491	dbSNP
rs1422416898	2497	dbSNP
rs965242552	2499	dbSNP
rs554065251	2509	dbSNP
rs1200311133	2517	dbSNP
rs1020583394	2526	dbSNP
rs372619819	2530	dbSNP
rs1206523238	2534	dbSNP
rs202239317	2535	dbSNP
rs572355467	2537	dbSNP
rs968683154	2549	dbSNP
rs978804878	2554	dbSNP
rs1229459230	2555	dbSNP
rs1302715540	2558	dbSNP
rs926989622	2559	dbSNP
rs1318968468	2563	dbSNP
rs1434913377	2571	dbSNP
rs1403340810	2572	dbSNP
rs1188555036	2573	dbSNP
rs1370244178	2574	dbSNP
rs1471884547	2585	dbSNP
rs1391560445	2588	dbSNP
rs937073568	2593	dbSNP
rs1163183444	2611	dbSNP
rs1256562066	2612	dbSNP
rs1272100746	2614	dbSNP
rs1424971300	2615	dbSNP
rs1467454369	2622	dbSNP
rs1169009463	2623	dbSNP
rs1397621250	2626	dbSNP
rs1344400623	2631	dbSNP
rs1390433432	2633	dbSNP
rs1334461070	2644	dbSNP
rs1226940269	2651	dbSNP
rs992576590	2653	dbSNP
rs1341732619	2656	dbSNP
rs572572728	2657	dbSNP
rs1292529834	2661	dbSNP
rs546080096	2662	dbSNP
rs1402046464	2663	dbSNP
rs1220189603	2670	dbSNP
rs940826694	2680	dbSNP
rs1036533030	2681	dbSNP
rs1255955202	2682	dbSNP
rs899387731	2683	dbSNP
rs954510662	2685	dbSNP
rs1201233547	2692	dbSNP
rs1368855922	2694	dbSNP
rs999398639	2701	dbSNP
rs541253228	2703	dbSNP
rs958330566	2706	dbSNP
rs187918518	2707	dbSNP
rs533494108	2708	dbSNP
rs1272534080	2713	dbSNP
rs916546539	2713	dbSNP
rs1186946496	2715	dbSNP
rs1264462584	2716	dbSNP
rs1267615630	2717	dbSNP
rs1289583109	2717	dbSNP
rs397997610	2717	dbSNP
rs564776546	2717	dbSNP
rs70997433	2717	dbSNP
rs889162510	2718	dbSNP
rs1008980763	2722	dbSNP
rs1351834963	2722	dbSNP
rs1019005703	2724	dbSNP
rs1164633688	2724	dbSNP
rs1456251670	2730	dbSNP
rs892557002	2736	dbSNP
rs1169209808	2742	dbSNP
rs1159869404	2746	dbSNP
rs1473508581	2751	dbSNP
rs928257794	2754	dbSNP
rs543664621	2757	dbSNP
rs1466932107	2761	dbSNP
rs1047637756	2774	dbSNP
rs1452409076	2775	dbSNP
rs562156247	2785	dbSNP
rs910052633	2787	dbSNP
rs576750568	2791	dbSNP
rs191223856	2792	dbSNP
rs1230801147	2795	dbSNP
rs1412211304	2795	dbSNP
rs1327630968	2799	dbSNP
rs1277270546	2802	dbSNP
rs1440927520	2810	dbSNP
rs1037468721	2811	dbSNP
rs1020130624	2818	dbSNP
rs553315800	2824	dbSNP
rs1438218288	2828	dbSNP
rs1393496192	2831	dbSNP
rs1320922200	2847	dbSNP
rs752584791	2848	dbSNP
rs1409574201	2852	dbSNP
rs900153454	2859	dbSNP
rs1379417712	2865	dbSNP
rs1197066900	2881	dbSNP
rs1341009530	2883	dbSNP
rs969025501	2884	dbSNP
rs1249053672	2885	dbSNP
rs1245633299	2898	dbSNP
rs375405207	2907	dbSNP
rs1401741945	2908	dbSNP
rs1284251718	2910	dbSNP
rs1220293207	2938	dbSNP
rs1000140021	2942	dbSNP
rs1345157735	2961	dbSNP
rs1276289701	2968	dbSNP
rs1231652548	2969	dbSNP
rs995866055	2973	dbSNP
rs1298061658	2978	dbSNP
rs1033974022	2980	dbSNP
rs958398983	2981	dbSNP
rs568177629	2982	dbSNP
rs1317608100	2984	dbSNP
rs1414933779	2987	dbSNP
rs916955575	2987	dbSNP
rs1404474833	2990	dbSNP

- Genes & development, 2013

When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.