miRTarBase - #MIRT641493


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pre-miRNA Information
pre-miRNA hsa-mir-3171   
Genomic Coordinates chr14: 27633205 - 27633278
Description Homo sapiens miR-3171 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-3171
Sequence 10| AGAUGUAUGGAAUCUGUAUAUAUC |33
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1317334268 4 dbSNP
rs1412633952 16 dbSNP
rs967845497 17 dbSNP
rs1278913737 19 dbSNP
rs1348410638 24 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol POLA2   
Synonyms -
Description DNA polymerase alpha 2, accessory subunit
Transcript NM_002689   
Expression
Putative miRNA Targets on POLA2
3'UTR of POLA2
(miRNA target sites are highlighted)		 
>POLA2|NM_002689|3'UTR
   1 GGCTTCTGTCCTCTGCTGTTCTCTGCTGTGTGGGCCCTTAAAGTCTTAGCCAAGAGCCAAGACATAGCCCTGTGACAAGG
  81 TGAACAGTTGGGTGGGAAAGGAGAGAGGAGCCAGCCAGGGAGGGGCAGCTGCAGTGACCAGGCCCAGCAGGGAGGACTTG
 161 TGCAGCCGGGCCTGCCTCTGAGTGGTGCCTCTCCTGGAAGGAAGCTCTTGCTTCTCAGTCCATGCTCCGTGTCCAGAAGT
 241 AAGCCAGCTGTGGATCCCGCCCACTCAGAAAAGGCGAGAAGGCTTTGTGATTTTCTACATGAATCAAACACAGAAACAAC
 321 TTTTGGAGAAATTAAATTCTGAGTGTGACTTCTGAGTTTGACCTTTCTTCTATTTTCTTTTATTTATTTATTTTGTTTTT
 401 AGACGGAGTCTCGATCTGTCGCCCAGGCTGGAGTGCAGTGGTGAGATCTCGGCTCACTGCAAGCTCTGCCGCCCGGGTTC
 481 ATGCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCACCCACCACCACGCCTGGCTAATTTTTTGTATTT
 561 TTAGTGGAGATGGGGTTTCACCATGTTAGCCAGGATGGTCTCGATCTCCTGACCTTGTGATCTGTCCGCCTCAGCCTCCC
 641 AAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCCCCTTTCTTCTATTTTCTTTATCCTGGTAGTAGACAAAGAA
 721 TGTTGCCTATTTGACCTATGTCCAGAGAAGGCAGAGTGTCAGTCACACTGGGTTTCTATCCAGCCCCAGTCCTGTCCAGT
 801 GGGTAACTCTCAGCCCCACTCCTCATCCCCCAAAGGCCCCAGTGACCTCTATGCTGCAGAGGTGCACTGGGTGGGCCCTA
 881 GAGCTGGGCTGCAGAGCAAGCAACTGGCTGCATGCCTGGGCCTCCCTGCGCAGTTCCTCACATGCATGCTGTGGTCCCAG
 961 CCTGACCTCTCCATCTGGAGGGGGCCGCCTCGCTGGGCTGTTCCAAAGCTGGAGTCCTGGTGGCAGCTGCTTTGCCAAGT
1041 CGCGCAGTAGCCAGGGCTTGCCTGCAGCCCAGAACACTCAGACAGGGTCAGGCCTTCCAGGGTTCACAGCACGTCCCATG
1121 CCCCTATGTCCTGGCATCTGGGGAAAAGCTGTCACTGGCCAGACGTGGAGTGGCTTCCTGCCCTAAGCTGGCAGGCTGTC
1201 CTGCCTCACATGCCAGAGCCAAGACCGGCAAACCCCAGCTGTCAGGGCAGGGAAGTGGGCAAGGGGGCAGCAAGCTGCTG
1281 GTGTCTATGCCTTGACTCCGAGATAAATAGATGACCTAACAGTGGCCAACTTTTGCCTCTCACCTTTCTGACGGTGAAAT
1361 GGAATCGAGCCCCAGACCTGACCAGGGTACAGTAAAAGGTCTTCCTTTAAACACAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' cuauAUAUGUCUA--AG-GUAUGUAga 5'
              |: :||| |  || |||:|||  
Target 5' tcccTGCGCAGTTCCTCACATGCATgc 3'
 923 - 949 122.00 -8.86
2 
miRNA  3' cuauAUAUGUCUAAGGUAUGUAga 5'
              | |:: ||||:: |||||  
Target 5' aggcTTTGT-GATTTTCTACATga 3'
 280 - 302 111.00 -5.50
3 
miRNA  3' cuAUAUAUG--UCUAAGG---UAUGUAga 5'
            | |||:|   || |||   ||| ||  
Target 5' tgTCTATGCCTTGACTCCGAGATAAATag 3'
 1282 - 1310 108.00 -9.40
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN16129438 7 COSMIC
COSN31543166 10 COSMIC
COSN30148037 14 COSMIC
COSN30106719 48 COSMIC
COSN30168942 102 COSMIC
COSN2481009 208 COSMIC
COSN15662609 361 COSMIC
COSN17756159 662 COSMIC
COSN28046040 825 COSMIC
COSN21819842 1295 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs759515899 2 dbSNP
rs199847515 4 dbSNP
rs949881118 7 dbSNP
rs751785556 11 dbSNP
rs4590878 14 dbSNP
rs1309646157 16 dbSNP
rs777493668 18 dbSNP
rs943213528 19 dbSNP
rs765269742 21 dbSNP
rs752599856 22 dbSNP
rs1044062201 29 dbSNP
rs762489143 38 dbSNP
rs763521404 41 dbSNP
rs751156997 45 dbSNP
rs1187355977 46 dbSNP
rs1228883689 60 dbSNP
rs1038900417 61 dbSNP
rs905567635 69 dbSNP
rs1294246493 70 dbSNP
rs904332816 78 dbSNP
rs935936799 96 dbSNP
rs1271057699 99 dbSNP
rs1053411546 108 dbSNP
rs1397406928 109 dbSNP
rs1456453505 111 dbSNP
rs41561615 113 dbSNP
rs994697347 113 dbSNP
rs1027544938 116 dbSNP
rs1410443105 155 dbSNP
rs372342062 156 dbSNP
rs1420051585 157 dbSNP
rs1250788562 158 dbSNP
rs1197239772 161 dbSNP
rs1466730173 162 dbSNP
rs1024037012 164 dbSNP
rs542289637 166 dbSNP
rs562456947 168 dbSNP
rs905631309 171 dbSNP
rs1001214474 172 dbSNP
rs1336200454 176 dbSNP
rs1017058322 177 dbSNP
rs8638 179 dbSNP
rs994466647 182 dbSNP
rs1181469716 186 dbSNP
rs1386833225 188 dbSNP
rs1463103327 191 dbSNP
rs1458130722 194 dbSNP
rs975266617 212 dbSNP
rs188243167 213 dbSNP
rs192780935 218 dbSNP
rs533656274 219 dbSNP
rs968064061 223 dbSNP
rs756471737 228 dbSNP
rs979652120 229 dbSNP
rs547350172 230 dbSNP
rs1317660606 231 dbSNP
rs11546563 232 dbSNP
rs911197728 243 dbSNP
rs1282061281 245 dbSNP
rs1204548617 247 dbSNP
rs1351102017 248 dbSNP
rs1262313701 249 dbSNP
rs957052023 253 dbSNP
rs566850487 254 dbSNP
rs992337405 255 dbSNP
rs979473503 259 dbSNP
rs535865410 260 dbSNP
rs189780475 276 dbSNP
rs184394146 277 dbSNP
rs569256903 280 dbSNP
rs538384824 281 dbSNP
rs1387812698 288 dbSNP
rs1171135692 290 dbSNP
rs1460851046 298 dbSNP
rs557660049 303 dbSNP
rs1394029813 311 dbSNP
rs913126434 314 dbSNP
rs1455185495 315 dbSNP
rs1239911901 324 dbSNP
rs949940763 329 dbSNP
rs926940053 336 dbSNP
rs1045389687 340 dbSNP
rs1447475436 343 dbSNP
rs930323967 344 dbSNP
rs1208390143 349 dbSNP
rs1346892557 362 dbSNP
rs779073215 363 dbSNP
rs577451566 385 dbSNP
rs189284056 391 dbSNP
rs1236827508 396 dbSNP
rs1258086850 405 dbSNP
rs1048929289 406 dbSNP
rs748276342 409 dbSNP
rs1370917167 413 dbSNP
rs1476121912 414 dbSNP
rs576277320 422 dbSNP
rs994728773 425 dbSNP
rs1166761140 428 dbSNP
rs1040198902 429 dbSNP
rs1025925201 440 dbSNP
rs1404684116 444 dbSNP
rs773096706 451 dbSNP
rs1468764194 452 dbSNP
rs901702146 468 dbSNP
rs1258010354 471 dbSNP
rs996256519 472 dbSNP
rs1403375815 473 dbSNP
rs1029096677 475 dbSNP
rs573263816 476 dbSNP
rs1341642517 498 dbSNP
rs1291965729 499 dbSNP
rs968008364 505 dbSNP
rs1356470453 508 dbSNP
rs1295460620 509 dbSNP
rs1000870986 514 dbSNP
rs1328368972 521 dbSNP
rs1219451957 525 dbSNP
rs1327775570 527 dbSNP
rs542222090 529 dbSNP
rs1398066353 539 dbSNP
rs1031048417 540 dbSNP
rs956971330 544 dbSNP
rs113888266 548 dbSNP
rs112879677 550 dbSNP
rs1018292115 551 dbSNP
rs773517472 554 dbSNP
rs1205560052 556 dbSNP
rs1439611539 565 dbSNP
rs969981079 567 dbSNP
rs979790763 568 dbSNP
rs927051807 569 dbSNP
rs1336043005 571 dbSNP
rs908896354 573 dbSNP
rs180923821 575 dbSNP
rs1048707671 580 dbSNP
rs907567227 584 dbSNP
rs1373148113 590 dbSNP
rs940605216 595 dbSNP
rs1378694140 604 dbSNP
rs1323248464 607 dbSNP
rs1460477320 610 dbSNP
rs1395039124 612 dbSNP
rs1167098921 614 dbSNP
rs1040716607 617 dbSNP
rs901652738 618 dbSNP
rs1471258770 620 dbSNP
rs1251397287 624 dbSNP
rs996420616 628 dbSNP
rs545127677 629 dbSNP
rs1275598688 633 dbSNP
rs1214355687 634 dbSNP
rs368468609 634 dbSNP
rs903936996 636 dbSNP
rs1225854578 641 dbSNP
rs913039877 646 dbSNP
rs1300809960 657 dbSNP
rs1000985113 661 dbSNP
rs1439514194 664 dbSNP
rs1371168500 669 dbSNP
rs1453519917 671 dbSNP
rs564504973 672 dbSNP
rs1157237719 673 dbSNP
rs1159505139 674 dbSNP
rs760844365 677 dbSNP
rs1400908974 678 dbSNP
rs1302766550 682 dbSNP
rs1173323924 683 dbSNP
rs1013815360 691 dbSNP
rs1454757105 701 dbSNP
rs533593862 710 dbSNP
rs1025663597 730 dbSNP
rs1184885073 739 dbSNP
rs1045588136 748 dbSNP
rs1343732659 751 dbSNP
rs547285769 753 dbSNP
rs969548479 759 dbSNP
rs1038149461 763 dbSNP
rs1347931135 764 dbSNP
rs1302713283 766 dbSNP
rs11826954 779 dbSNP
rs1361118755 781 dbSNP
rs1289382847 784 dbSNP
rs529258559 786 dbSNP
rs73484989 790 dbSNP
rs776463251 791 dbSNP
rs1435525098 794 dbSNP
rs566261818 796 dbSNP
rs891261427 800 dbSNP
rs1427996511 814 dbSNP
rs984533805 815 dbSNP
rs1008493289 825 dbSNP
rs1188859641 832 dbSNP
rs760144141 836 dbSNP
rs940368248 852 dbSNP
rs1212086046 854 dbSNP
rs1485767327 855 dbSNP
rs1489658707 856 dbSNP
rs899856861 860 dbSNP
rs1001261333 865 dbSNP
rs1267406350 892 dbSNP
rs538334673 894 dbSNP
rs923184678 895 dbSNP
rs1032315433 907 dbSNP
rs1292390053 913 dbSNP
rs1157548371 919 dbSNP
rs147258376 923 dbSNP
rs11538533 926 dbSNP
rs537939029 929 dbSNP
rs1050243773 930 dbSNP
rs752143124 931 dbSNP
rs1355238115 935 dbSNP
rs1173419653 938 dbSNP
rs1474490193 946 dbSNP
rs1363901137 950 dbSNP
rs1426761632 953 dbSNP
rs936723982 955 dbSNP
rs1052542123 959 dbSNP
rs1417803074 960 dbSNP
rs892440400 966 dbSNP
rs1189026028 967 dbSNP
rs189353467 968 dbSNP
rs1294237221 975 dbSNP
rs1014010036 980 dbSNP
rs1175181963 985 dbSNP
rs1025183764 987 dbSNP
rs372415280 988 dbSNP
rs1390809330 992 dbSNP
rs1332511854 993 dbSNP
rs905439044 1010 dbSNP
rs1233669657 1017 dbSNP
rs981360862 1028 dbSNP
rs1305102981 1030 dbSNP
rs762544569 1042 dbSNP
rs533757198 1043 dbSNP
rs952904603 1044 dbSNP
rs1459954977 1045 dbSNP
rs1394305760 1051 dbSNP
rs937039187 1057 dbSNP
rs1223268449 1069 dbSNP
rs1459759446 1077 dbSNP
rs1156460333 1084 dbSNP
rs1362474292 1084 dbSNP
rs1265583022 1094 dbSNP
rs974160388 1099 dbSNP
rs984481552 1102 dbSNP
rs1179895218 1103 dbSNP
rs919581176 1112 dbSNP
rs1015100085 1113 dbSNP
rs553607468 1114 dbSNP
rs542070318 1124 dbSNP
rs1258695326 1125 dbSNP
rs944185582 1129 dbSNP
rs1440730246 1138 dbSNP
rs922997690 1147 dbSNP
rs1317683728 1156 dbSNP
rs117341061 1160 dbSNP
rs1383740793 1165 dbSNP
rs986071313 1166 dbSNP
rs900098572 1177 dbSNP
rs941725932 1178 dbSNP
rs1202026005 1179 dbSNP
rs925085293 1191 dbSNP
rs936505193 1193 dbSNP
rs1383482972 1196 dbSNP
rs1052498819 1202 dbSNP
rs1454631545 1203 dbSNP
rs1460472833 1211 dbSNP
rs1296417655 1219 dbSNP
rs1032759347 1220 dbSNP
rs369118672 1227 dbSNP
rs572231587 1228 dbSNP
rs1046821049 1238 dbSNP
rs1314154702 1242 dbSNP
rs1380299062 1245 dbSNP
rs1025010371 1261 dbSNP
rs1259591418 1263 dbSNP
rs905230727 1265 dbSNP
rs1488533944 1275 dbSNP
rs1288413472 1281 dbSNP
rs1232433645 1282 dbSNP
rs555875801 1288 dbSNP
rs1229729676 1291 dbSNP
rs867307903 1299 dbSNP
rs1002391855 1301 dbSNP
rs576021162 1303 dbSNP
rs888585350 1304 dbSNP
rs1325125955 1307 dbSNP
rs1318318853 1308 dbSNP
rs1383111631 1317 dbSNP
rs1362898790 1322 dbSNP
rs1299616283 1323 dbSNP
rs1004341550 1330 dbSNP
rs544755502 1334 dbSNP
rs1374497797 1338 dbSNP
rs1015803011 1340 dbSNP
rs1034118253 1344 dbSNP
rs962094030 1353 dbSNP
rs558707796 1354 dbSNP
rs373182642 1355 dbSNP
rs1265118877 1357 dbSNP
rs1203583017 1367 dbSNP
rs973978433 1368 dbSNP
rs1202241276 1376 dbSNP
rs997304948 1378 dbSNP
rs1270097810 1381 dbSNP
rs919594800 1385 dbSNP
rs1241748693 1387 dbSNP
rs540864209 1390 dbSNP
rs982978304 1394 dbSNP
rs1291776654 1397 dbSNP
rs1414555959 1399 dbSNP
rs1374409884 1407 dbSNP
rs1473947266 1409 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084066. RNA binding protein: AGO2. Condition:CLIP_noemetine_SantaCruzAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' cuaUAUAUGUCUAAGGUAUGUaga 5'
             ||| |::|| | ||||||   
Target 5' ---AUAAAUGGAAU-CAUACAaua 3'
 		1 - 20
Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084066
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_noemetine_SantaCruzAb
Location of target site ENST00000265465.3 | 3UTR | AUAAAUGGAAUCAUACAAUAC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
Click to see details
37 hsa-miR-3171 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT117345 MAPRE2 microtubule associated protein RP/EB family member 2 2 2
MIRT152426 ARID3A AT-rich interaction domain 3A 2 2
MIRT227670 SET SET nuclear proto-oncogene 2 2
MIRT293606 PVR poliovirus receptor 2 2
MIRT442923 TFDP2 transcription factor Dp-2 2 2
MIRT447007 RSF1 remodeling and spacing factor 1 2 2
MIRT450195 ZHX3 zinc fingers and homeoboxes 3 2 2
MIRT450883 COL4A5 collagen type IV alpha 5 chain 2 2
MIRT459707 ZNF641 zinc finger protein 641 2 2
MIRT467111 SRI sorcin 2 2
MIRT468271 SFXN1 sideroflexin 1 2 2
MIRT484168 WDR82P1 WD repeat domain 82 pseudogene 1 2 8
MIRT502274 H3F3B H3 histone family member 3B 2 4
MIRT504190 FAM127B retrotransposon Gag like 8A 2 2
MIRT507718 CMPK1 cytidine/uridine monophosphate kinase 1 2 6
MIRT513852 JARID2 jumonji and AT-rich interaction domain containing 2 2 8
MIRT521900 PIAS1 protein inhibitor of activated STAT 1 2 2
MIRT525975 SPA17 sperm autoantigenic protein 17 2 2
MIRT534599 RORA RAR related orphan receptor A 2 2
MIRT537572 ESYT2 extended synaptotagmin 2 2 2
MIRT539261 ANKRD44 ankyrin repeat domain 44 2 2
MIRT544214 SRSF11 serine and arginine rich splicing factor 11 2 4
MIRT553877 SUPT7L SPT7 like, STAGA complex gamma subunit 2 4
MIRT564547 CCDC80 coiled-coil domain containing 80 2 2
MIRT571228 SYT9 synaptotagmin 9 2 2
MIRT572016 H3F3C H3 histone family member 3C 2 2
MIRT613116 EIF4EBP2 eukaryotic translation initiation factor 4E binding protein 2 2 2
MIRT629028 MMP16 matrix metallopeptidase 16 2 4
MIRT635228 CD59 CD59 molecule (CD59 blood group) 2 2
MIRT641493 POLA2 DNA polymerase alpha 2, accessory subunit 2 2
MIRT648008 ZNF431 zinc finger protein 431 2 2
MIRT697344 ZNF544 zinc finger protein 544 2 2
MIRT700991 PDE3A phosphodiesterase 3A 2 2
MIRT704714 CHD9 chromodomain helicase DNA binding protein 9 2 2
MIRT705320 ATP2A2 ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 2 2
MIRT705387 ATP1B3 ATPase Na+/K+ transporting subunit beta 3 2 2
MIRT715811 NUDT7 nudix hydrolase 7 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-3171 Ceritinib 57379345 NSC776422 approved resistant cell line (H3122)
hsa-miR-3171 Gefitinib 123631 NSC715055 approved sensitive cell line (HCC827)
hsa-miR-3171 Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-3171 Ceritinib 57379345 NSC776422 approved resistant cell line (H3122)

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