pre-miRNA Information | |
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pre-miRNA | hsa-mir-452 |
Genomic Coordinates | chrX: 151959628 - 151959712 |
Synonyms | MIRN452, hsa-mir-452, MIR452 |
Description | Homo sapiens miR-452 stem-loop |
Comment | The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies . The 5' end of the miRNA may be offset with respect to previous annotations. |
RNA Secondary Structure | ![]() |
Associated Diseases | ![]() |
Mature miRNA Information | |||||||||||||||||||
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Mature miRNA | hsa-miR-452-3p | ||||||||||||||||||
Sequence | 58| CUCAUCUGCAAAGAAGUAAGUG |79 | ||||||||||||||||||
Evidence | Experimental | ||||||||||||||||||
Experiments | Array-cloned | ||||||||||||||||||
Editing Events in miRNAs |
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SNPs in miRNA |
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Putative Targets |
miRNA Expression profile | |
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miRNAs in Extracellular Vesicles |
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Circulating MicroRNA Expression Profiling |
Gene Information | |||||||||||||||||||||
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Gene Symbol | SAR1A | ||||||||||||||||||||
Synonyms | SAR1, SARA1, Sara, masra2 | ||||||||||||||||||||
Description | secretion associated Ras related GTPase 1A | ||||||||||||||||||||
Transcript | NM_001142648 | ||||||||||||||||||||
Other Transcripts | NM_020150 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on SAR1A | |||||||||||||||||||||
3'UTR of SAR1A (miRNA target sites are highlighted) |
>SAR1A|NM_001142648|3'UTR 1 TGTTTGGACGGTGAAAATAAAAGAGTTTTACTTCTCTGGACTGATCCTATTCACAGCTTCCTCATGAACTTTTCTAATAG 81 AACAAGGAAAGCTCTCCAACCATGTCTGGCGTTGAGAAGCCAAGAGTCTCTGTCAACTCTCTCATTGCCCAGTGGTGACA 161 TGTGCTCTTCTCCACACTGTTGGGAGGTAATGCTGCCCCACGTGCTGGTGCAGGTCAGTATCCTGGGACTTGGAAGCTGG 241 CAGGATTTGCCGGGTAAAGCTGTATGCCATCATGGGGCACCTGAAAAGAAAAACACGTCTCACCACTGTGGTTGATTCAA 321 AAGAAAGTGATTCTATTTTTTAAAGAAAGCGTTGTTAATGTAATTGGTATCCCTCCTAACTTTTTGAGTTCACAATTTAC 401 TTGGTCCAGAGTTTTCTATTCTTTTTTTTTTTTAAACTAATGAATGACATTTAGATACTTCATAAAATTATGAACAGATA 481 TGGAGGCCAGAGCTCATTTGGGTAAACTTACTCCTGCTGAGTTAGCAGGTTGGTGAGAGAAGCTCCCCTGAGCTCACCTG 561 TCTCTCTGACTGCCTTGGAGTAGGTGGCATAACCTTGTGCACAGAGAACTAGAAAAGGGGCAGAACCCCGGCCTTGCAGT 641 TGTGGCAGGTTTCCACTGTGGTAAGCTAGGTTCATTCCTCATCAAGGAATGTGTAGCAGATTGTTCACTGTGGAGGAGTT 721 AATTATAGAATGGGTTATTGTTGTTATTCTTACTCATGAAGTTACAGATTTTAGCCAGTCTTTGCTTTTATACTTTTGTG 801 AAATTTAATTTCTCTCTATAGCACCTTCCTTTTTCGTTTTCAGTTATCAAAAGTGACTTTGACCTCATAAAAGAGTTGAG 881 AACATCTCTCGTGTCACATACTGCAGGTGCATCAGTTACTTTTGCACAGATTCTAGGGGGACATTTTTCTGAATAGGAAG 961 ACAGGACAAAGTTAACAGCTTAAGGGCTCTTAATTCTGTGAGTTGAGGACTTAAAAGTATTGTAGCATTTGTTTGGATCC 1041 ATGAAAAATGTATTCAGTGGGCTTTAAAATTTCCATTTGCAGAATTTGGTCTCTCAGGCTGTTTGGGAGCTCTTTTTTTT 1121 ACATTTTTTCTCCTTTGACACCTATTTTATTGGTGTTTAAAGTAAAGGTTAACATCTGTAGCTTTTCCAGGTTTTTTTTT 1201 TTTTTTTTTTTTTTTTGGTATGAAATTGTCTTTCTCCATTGCAGAAATAAGCTAGGGAAACACTAACCCAAAAACTTTCT 1281 GTAGAGCTGTTCCTTTGGAGGCAGCATCACTTATTGGCAGTAAAGACTCAGTATAAAAGCACCGGCATCCCTACTTGGGT 1361 GATGGGGATTAATTTTATAGCATTCCATTTTCCTAGTGCCACATGTGAAATTGGATTTTGATGATCTTAATCTATATTCT 1441 ACCCTTATAATAAAAGATCAAAAGATATATCTCCTATGAACAGATTGGAGATAGGAGATGAAAAGTTGGGAGGATGTCTT 1521 TATTCTAATGTGAGGGTAGGGAAAATGTGGATAACATTACTGGGGTGAGGGAGGCATTGTTCTTTAGTTGGAGTTCTCAT 1601 TCTTATTCTCCAGTACTGACTTGTGGGGAAAGCATACTTTTTCACTGCCAGGTACTGAATGCAGAGGCTCAGTGAAGTAT 1681 ATATGTGGGAAGTGCATGCATTTCGTTTATTAGCAAACATAGCTGGATTAAGACAAAGTTGTTGGTTTGGAAAGGGGTTA 1761 AAGCCTTAAGTGAACAAATCTAGCTAACAGTGAATGAACTAGGTAATATAACTTGCATATTTTTAATTTCCTTTGGTTAA 1841 AGGTCCCCCATACTTCTCTGTTCGGAGACATGAGAAGTATGATTACTTCAGTGTTAGTTTTCTTAATTTTTTTTTTCCCC 1921 TATTTGTCCCTTGTCACTTTGTTGCAAGCTAGAAATCTGTGGGTTATACATAGGGCAGCTCTTTGTGAAAGTGGTTTATT 2001 CCACTGGAGAAAGGGGATTGAAAATCAGTTAGAACCAATGTATTTCTTGCCCCACGGAACACTATTCCTATAAGATAGCT 2081 GAAAGAAGCTGCTGTGAGGAGCTCAGCTCCAAACACAGGATCAGCACCTTGTATAGGAATTCCCATGAATTATGACTTCT 2161 CATTCTGTTTTATCAGAGTGCATATATGTCCTACTTCAGGAAAAGTAAAACAGTCATTTACGAAAGAAAGTCAATCTGTA 2241 TCCTAAGCATTTTAATAAAAAGTTAAAACAAAAAAAAAAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
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miRNA:Target | ---- | ||||||
Validation Method |
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Conditions | HEK293S | ||||||
Location of target site | 3'UTR | ||||||
Tools used in this research | TargetScan , miRTarCLIP , Piranha | ||||||
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM1084064. RNA binding protein: AGO2. Condition:CLIP_noemetine_AbnovaAb
HITS-CLIP data was present in GSM1084065. RNA binding protein: AGO2. Condition:CLIP_emetine_AbnovaAb
... - Karginov FV; Hannon GJ, 2013, Genes & development. |
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miRNA-target interactions (Provided by authors) |
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Article |
- Karginov FV; Hannon GJ - Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
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CLIP-seq Support 1 for dataset GSM1084064 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_noemetine_AbnovaAb |
Location of target site | ENST00000373242.2 | 3UTR | AAAAGUUGGGAGGAUGU |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset GSM1084065 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_emetine_AbnovaAb |
Location of target site | ENST00000373242.2 | 3UTR | AGGAGAUGAAAAGUUGGGAGGAUGU |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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MiRNA-Target Expression Profile (TCGA) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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87 hsa-miR-452-3p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT150000 | MIDN | midnolin | ![]() |
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2 | 10 | ||||||
MIRT330600 | ZWINT | ZW10 interacting kinetochore protein | ![]() |
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2 | 2 | ||||||
MIRT358701 | SUB1 | SUB1 homolog, transcriptional regulator | ![]() |
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2 | 4 | ||||||
MIRT362854 | EIF4H | eukaryotic translation initiation factor 4H | ![]() |
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2 | 2 | ||||||
MIRT447215 | ATXN7 | ataxin 7 | ![]() |
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2 | 2 | ||||||
MIRT466655 | TAF1D | TATA-box binding protein associated factor, RNA polymerase I subunit D | ![]() |
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2 | 6 | ||||||
MIRT483979 | PANK1 | pantothenate kinase 1 | ![]() |
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2 | 8 | ||||||
MIRT485118 | SF3B3 | splicing factor 3b subunit 3 | ![]() |
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2 | 2 | ||||||
MIRT488864 | AUTS8 | Autism, susceptibility to, 8 | ![]() |
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2 | 2 | ||||||
MIRT492293 | SH2B3 | SH2B adaptor protein 3 | ![]() |
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2 | 2 | ||||||
MIRT492976 | NCK2 | NCK adaptor protein 2 | ![]() |
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2 | 2 | ||||||
MIRT497410 | LRRC40 | leucine rich repeat containing 40 | ![]() |
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2 | 2 | ||||||
MIRT511143 | MRPL17 | mitochondrial ribosomal protein L17 | ![]() |
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2 | 6 | ||||||
MIRT512650 | MAP3K2 | mitogen-activated protein kinase kinase kinase 2 | ![]() |
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2 | 2 | ||||||
MIRT513017 | NSFL1C | NSFL1 cofactor | ![]() |
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2 | 6 | ||||||
MIRT520445 | TSPAN2 | tetraspanin 2 | ![]() |
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2 | 6 | ||||||
MIRT527995 | NDNF | neuron derived neurotrophic factor | ![]() |
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2 | 2 | ||||||
MIRT528667 | PDE4DIP | phosphodiesterase 4D interacting protein | ![]() |
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2 | 2 | ||||||
MIRT533512 | TRIM71 | tripartite motif containing 71 | ![]() |
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2 | 2 | ||||||
MIRT537404 | FBXO47 | F-box protein 47 | ![]() |
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2 | 2 | ||||||
MIRT538161 | DCP2 | decapping mRNA 2 | ![]() |
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2 | 2 | ||||||
MIRT539124 | ARHGEF17 | Rho guanine nucleotide exchange factor 17 | ![]() |
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2 | 2 | ||||||
MIRT540666 | MIS18A | MIS18 kinetochore protein A | ![]() |
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2 | 4 | ||||||
MIRT542948 | GDF11 | growth differentiation factor 11 | ![]() |
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2 | 2 | ||||||
MIRT547534 | MAML3 | mastermind like transcriptional coactivator 3 | ![]() |
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2 | 2 | ||||||
MIRT559033 | C20orf24 | chromosome 20 open reading frame 24 | ![]() |
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2 | 4 | ||||||
MIRT559554 | ARF6 | ADP ribosylation factor 6 | ![]() |
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2 | 2 | ||||||
MIRT570177 | RCBTB1 | RCC1 and BTB domain containing protein 1 | ![]() |
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2 | 2 | ||||||
MIRT573149 | ITGA9 | integrin subunit alpha 9 | ![]() |
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2 | 2 | ||||||
MIRT575849 | Rab1 | RAB1A, member RAS oncogene family | ![]() |
1 | 1 | |||||||
MIRT611034 | RRP1B | ribosomal RNA processing 1B | ![]() |
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2 | 2 | ||||||
MIRT616281 | HMGB1 | high mobility group box 1 | ![]() |
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2 | 2 | ||||||
MIRT618372 | PRKG2 | protein kinase, cGMP-dependent, type II | ![]() |
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2 | 2 | ||||||
MIRT619540 | PIWIL2 | piwi like RNA-mediated gene silencing 2 | ![]() |
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2 | 2 | ||||||
MIRT622303 | SGIP1 | SH3 domain GRB2 like endophilin interacting protein 1 | ![]() |
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2 | 2 | ||||||
MIRT622590 | PRRG4 | proline rich and Gla domain 4 | ![]() |
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2 | 2 | ||||||
MIRT624441 | CAMK2N1 | calcium/calmodulin dependent protein kinase II inhibitor 1 | ![]() |
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2 | 2 | ||||||
MIRT637089 | KLRD1 | killer cell lectin like receptor D1 | ![]() |
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2 | 2 | ||||||
MIRT639084 | ADCYAP1 | adenylate cyclase activating polypeptide 1 | ![]() |
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2 | 2 | ||||||
MIRT639334 | NINJ1 | ninjurin 1 | ![]() |
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2 | 2 | ||||||
MIRT639798 | EIF3E | eukaryotic translation initiation factor 3 subunit E | ![]() |
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2 | 2 | ||||||
MIRT640851 | RAB3B | RAB3B, member RAS oncogene family | ![]() |
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2 | 2 | ||||||
MIRT641628 | KIAA1244 | ARFGEF family member 3 | ![]() |
1 | 1 | |||||||
MIRT642634 | EPPIN | epididymal peptidase inhibitor | ![]() |
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2 | 2 | ||||||
MIRT643053 | EPPIN-WFDC6 | EPPIN-WFDC6 readthrough | ![]() |
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2 | 2 | ||||||
MIRT643416 | ERVMER34-1 | endogenous retrovirus group MER34 member 1, envelope | ![]() |
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2 | 2 | ||||||
MIRT645586 | SAR1A | secretion associated Ras related GTPase 1A | ![]() |
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2 | 2 | ||||||
MIRT647393 | FAM181B | family with sequence similarity 181 member B | ![]() |
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2 | 2 | ||||||
MIRT649411 | CDC14B | cell division cycle 14B | ![]() |
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2 | 2 | ||||||
MIRT649477 | CLDN16 | claudin 16 | ![]() |
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2 | 2 | ||||||
MIRT651307 | ZDHHC20 | zinc finger DHHC-type containing 20 | ![]() |
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2 | 2 | ||||||
MIRT651387 | ZBTB16 | zinc finger and BTB domain containing 16 | ![]() |
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2 | 2 | ||||||
MIRT653411 | SLC7A2 | solute carrier family 7 member 2 | ![]() |
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2 | 2 | ||||||
MIRT654084 | RSPH4A | radial spoke head 4 homolog A | ![]() |
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2 | 2 | ||||||
MIRT654613 | PTPRM | protein tyrosine phosphatase, receptor type M | ![]() |
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2 | 2 | ||||||
MIRT655338 | PCP4L1 | Purkinje cell protein 4 like 1 | ![]() |
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2 | 2 | ||||||
MIRT656173 | MRPL44 | mitochondrial ribosomal protein L44 | ![]() |
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2 | 2 | ||||||
MIRT657272 | HS3ST3B1 | heparan sulfate-glucosamine 3-sulfotransferase 3B1 | ![]() |
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2 | 2 | ||||||
MIRT657881 | GFPT1 | glutamine--fructose-6-phosphate transaminase 1 | ![]() |
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2 | 2 | ||||||
MIRT659875 | CAPRIN1 | cell cycle associated protein 1 | ![]() |
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2 | 2 | ||||||
MIRT662381 | ICA1L | islet cell autoantigen 1 like | ![]() |
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2 | 4 | ||||||
MIRT666308 | SLC22A3 | solute carrier family 22 member 3 | ![]() |
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2 | 2 | ||||||
MIRT667274 | NAV1 | neuron navigator 1 | ![]() |
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2 | 2 | ||||||
MIRT667614 | LIMCH1 | LIM and calponin homology domains 1 | ![]() |
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2 | 2 | ||||||
MIRT674242 | NUP62 | nucleoporin 62 | ![]() |
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2 | 4 | ||||||
MIRT690386 | PARP15 | poly(ADP-ribose) polymerase family member 15 | ![]() |
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2 | 2 | ||||||
MIRT693402 | NUDT16 | nudix hydrolase 16 | ![]() |
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2 | 2 | ||||||
MIRT702503 | KDELR1 | KDEL endoplasmic reticulum protein retention receptor 1 | ![]() |
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2 | 2 | ||||||
MIRT708632 | STMN4 | stathmin 4 | ![]() |
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2 | 2 | ||||||
MIRT708839 | SCAND3 | zinc finger BED-type containing 9 | ![]() |
1 | 1 | |||||||
MIRT709016 | HSBP1 | heat shock factor binding protein 1 | ![]() |
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2 | 2 | ||||||
MIRT709235 | RANGAP1 | Ran GTPase activating protein 1 | ![]() |
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2 | 2 | ||||||
MIRT710885 | PARL | presenilin associated rhomboid like | ![]() |
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2 | 2 | ||||||
MIRT712262 | PPP1CB | protein phosphatase 1 catalytic subunit beta | ![]() |
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2 | 2 | ||||||
MIRT712573 | ATP2B4 | ATPase plasma membrane Ca2+ transporting 4 | ![]() |
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2 | 2 | ||||||
MIRT715046 | PRPF38A | pre-mRNA processing factor 38A | ![]() |
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2 | 2 | ||||||
MIRT718905 | GALR1 | galanin receptor 1 | ![]() |
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2 | 2 | ||||||
MIRT719368 | FEM1A | fem-1 homolog A | ![]() |
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2 | 2 | ||||||
MIRT719495 | SEC24B | SEC24 homolog B, COPII coat complex component | ![]() |
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2 | 2 | ||||||
MIRT719601 | PRKX | protein kinase, X-linked | ![]() |
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2 | 2 | ||||||
MIRT719891 | RRP36 | ribosomal RNA processing 36 | ![]() |
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2 | 2 | ||||||
MIRT720063 | ZNF449 | zinc finger protein 449 | ![]() |
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2 | 2 | ||||||
MIRT721679 | CMTM4 | CKLF like MARVEL transmembrane domain containing 4 | ![]() |
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2 | 2 | ||||||
MIRT721724 | VTI1A | vesicle transport through interaction with t-SNAREs 1A | ![]() |
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2 | 2 | ||||||
MIRT723370 | ZNF470 | zinc finger protein 470 | ![]() |
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2 | 2 | ||||||
MIRT724941 | TXNL1 | thioredoxin like 1 | ![]() |
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2 | 2 | ||||||
MIRT725302 | NLRC5 | NLR family CARD domain containing 5 | ![]() |
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2 | 2 |
miRNA-Drug Associations | |||||||||||||||||||||||||||||||||||||||||||||
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miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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