pre-miRNA Information
pre-miRNA hsa-mir-378g   
Genomic Coordinates chr1: 94745860 - 94745900
Description Homo sapiens miR-378g stem-loop
Comment None
RNA Secondary Structure
Associated Diseases

Mature miRNA Information
Mature miRNA hsa-miR-378g
Sequence 2| ACUGGGCUUGGAGUCAGAAG |21
Evidence Experimental
Experiments Illumina
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol MGST3   
Synonyms GST-III
Description microsomal glutathione S-transferase 3
Transcript NM_004528   
Expression
Putative miRNA Targets on MGST3
3'UTR of MGST3
(miRNA target sites are highlighted)
>MGST3|NM_004528|3'UTR
   1 AGAATTATAGGGGTTTAAAAACTCTCATTCATTTTAAATGACTTACCTTTATTTCCAGTTACATTTTTTTTCTAAATATA
  81 ATAAAAACTTACCTGGCATCAGCCTCATACCTAAAACTCCTGACTCTTACCACTCATTTCCGTTTGAGTCTGTATCTGAA
 161 ATCAGTAGCCTAGTCCTACTAGATGAGAAAGGAGCCACAAGTATTGTGCCCTCTCCTCACCCTTCCAGCAGATGCTTCTG
 241 TAGTATGTGAGGTTGAGAAAAAGTCTGATTGTGGTGATGTAGGTATAGTCATGCCACAGTGATGAAAAATTAAAGAAAAA
 321 TCTTCTAGCTCTCAGGATATGCATTATCACTTGCTACAGATACTCCAAGGCCAAAGGAATGCTTGAGCCTAAAAGTTCAA
 401 GACCAGCCTGGGCAACATAGCAAAACCCCATCTCTACAAAAAAATATACAACAGTTAGCCAGGCATGATGGCACATGTCT
 481 GCAGTCCCAGCTACTTGGGAGGCTAAGGTGGGAGGATCACTTGAGCCCAGGGTGTCAAGGCTGCAGTGAGTTATGATCAC
 561 ACTGCTGCACTCCAGCGTGGGTGACAGAGCAAGACCCTGTCTCAAATAAATAAAAATTAAGTTTCTATTAAAAAAAAAAA
 641 A
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' gaagACUGAGGUUCGGGUCa 5'
              | ||| ::||||||| 
Target 5' aggaTCACT-TGAGCCCAGg 3'
513 - 531 151.00 -12.80
2
miRNA  3' gaAGACUGAGGU--UCGGGUCa 5'
            |||||  :||  ||||:|| 
Target 5' taTCTGAAATCAGTAGCCTAGt 3'
153 - 174 144.00 -17.90
3
miRNA  3' gaagacUGAGGUUCGGGUCa 5'
                | |:|||| |||| 
Target 5' cctaaaAGTTCAAGACCAGc 3'
388 - 407 126.00 -11.60
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30121608 3 COSMIC
COSN30447533 25 COSMIC
COSN30109797 41 COSMIC
COSN30497089 46 COSMIC
COSN30495855 72 COSMIC
COSN30747811 72 COSMIC
COSN7182309 97 COSMIC
COSN32083113 342 COSMIC
COSN1411201 418 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1414609614 4 dbSNP
rs766507497 9 dbSNP
rs763840126 13 dbSNP
rs751765754 14 dbSNP
rs1458048046 17 dbSNP
rs1323005378 23 dbSNP
rs1381141854 28 dbSNP
rs552488954 43 dbSNP
rs1285366928 45 dbSNP
rs373059941 47 dbSNP
rs755972776 48 dbSNP
rs8133 59 dbSNP
rs531753887 63 dbSNP
rs888063059 64 dbSNP
rs9333486 64 dbSNP
rs75462110 65 dbSNP
rs10102 73 dbSNP
rs1323308841 75 dbSNP
rs1378429849 76 dbSNP
rs1018644468 77 dbSNP
rs964976951 79 dbSNP
rs1334399552 82 dbSNP
rs1196522785 92 dbSNP
rs1247440863 98 dbSNP
rs1261503884 101 dbSNP
rs1192101861 108 dbSNP
rs187363916 115 dbSNP
rs1027909791 127 dbSNP
rs1251315359 133 dbSNP
rs952277267 134 dbSNP
rs534328458 136 dbSNP
rs9333487 142 dbSNP
rs547905565 143 dbSNP
rs1225178087 155 dbSNP
rs939464325 157 dbSNP
rs570688211 162 dbSNP
rs916615860 167 dbSNP
rs1260101333 171 dbSNP
rs376691545 176 dbSNP
rs948661072 180 dbSNP
rs1044664592 186 dbSNP
rs1198295218 189 dbSNP
rs1248679727 191 dbSNP
rs904445447 193 dbSNP
rs966816804 199 dbSNP
rs1447023498 210 dbSNP
rs765928799 215 dbSNP
rs935803838 221 dbSNP
rs9333488 225 dbSNP
rs1167859618 235 dbSNP
rs1460948901 240 dbSNP
rs1372944288 244 dbSNP
rs1368755934 246 dbSNP
rs576228072 250 dbSNP
rs1435341793 251 dbSNP
rs891531751 252 dbSNP
rs1008679980 253 dbSNP
rs34861431 258 dbSNP
rs535442788 258 dbSNP
rs1458345090 269 dbSNP
rs191699838 281 dbSNP
rs4147611 283 dbSNP
rs1194080505 286 dbSNP
rs1480275049 288 dbSNP
rs912449519 299 dbSNP
rs781671733 313 dbSNP
rs1297321721 316 dbSNP
rs1302389248 330 dbSNP
rs1393863601 331 dbSNP
rs943708640 332 dbSNP
rs184727128 336 dbSNP
rs1335280604 354 dbSNP
rs1390258800 357 dbSNP
rs1402282625 360 dbSNP
rs1303970244 367 dbSNP
rs189225437 377 dbSNP
rs1355168268 383 dbSNP
rs1393680091 383 dbSNP
rs9333489 397 dbSNP
rs931070350 398 dbSNP
rs952351433 409 dbSNP
rs140040297 411 dbSNP
rs1004991551 419 dbSNP
rs563202034 422 dbSNP
rs1418647343 429 dbSNP
rs1256917226 438 dbSNP
rs1202155780 445 dbSNP
rs531867339 447 dbSNP
rs1484623353 448 dbSNP
rs1279383101 455 dbSNP
rs1318777716 459 dbSNP
rs1226764130 462 dbSNP
rs1355428362 473 dbSNP
rs1277473764 475 dbSNP
rs1237876274 476 dbSNP
rs1015076462 477 dbSNP
rs1314178777 480 dbSNP
rs1249627823 482 dbSNP
rs1484360420 500 dbSNP
rs960772195 527 dbSNP
rs777910919 528 dbSNP
rs542496705 529 dbSNP
rs916670649 531 dbSNP
rs561985305 532 dbSNP
rs9333490 533 dbSNP
rs1157089363 541 dbSNP
rs547945580 544 dbSNP
rs1469799084 545 dbSNP
rs1369151966 547 dbSNP
rs1185769310 548 dbSNP
rs9333491 549 dbSNP
rs1014044197 551 dbSNP
rs1425687873 555 dbSNP
rs1194286541 556 dbSNP
rs925839279 563 dbSNP
rs1489189081 577 dbSNP
rs935956576 578 dbSNP
rs570922492 586 dbSNP
rs1356560076 595 dbSNP
rs1370777791 599 dbSNP
rs1266701000 608 dbSNP
rs902627211 612 dbSNP
rs1354253543 614 dbSNP
rs1052925339 621 dbSNP
rs576637041 624 dbSNP
rs1401111032 628 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084065. RNA binding protein: AGO2. Condition:CLIP_emetine_AbnovaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM4903836
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / 124_TD_21_a
Location of target site NM_004528 | 3UTR | GUGGGAGGAUCACUUGAGCCCAGG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM4903837
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / 124_TD_21_b
Location of target site NM_004528 | 3UTR | CUUGGGAGGCUAAGGUGGGAGGAUCACUUGAGCCCAGG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM4903838
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / 124_TD_21_c
Location of target site NM_004528 | 3UTR | ACUUGGGAGGCUAAGGUGGGAGGAUCACUUGAGCCCAGG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM1084065
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_emetine_AbnovaAb
Location of target site ENST00000367889.3 | 3UTR | CUAAGGUGGGAGGAUCACUUGAGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
68 hsa-miR-378g Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT444739 SMYD1 SET and MYND domain containing 1 2 2
MIRT456104 VAV3 vav guanine nucleotide exchange factor 3 2 4
MIRT458683 MRI1 methylthioribose-1-phosphate isomerase 1 2 2
MIRT465802 TMEM91 transmembrane protein 91 2 2
MIRT470843 PLXND1 plexin D1 2 2
MIRT497263 GRK6 G protein-coupled receptor kinase 6 2 2
MIRT497674 SYNGR1 synaptogyrin 1 2 2
MIRT498218 TLN2 talin 2 2 2
MIRT498309 BCL11B B-cell CLL/lymphoma 11B 2 2
MIRT504046 TOMM5 translocase of outer mitochondrial membrane 5 2 2
MIRT518196 CLEC4E C-type lectin domain family 4 member E 2 2
MIRT533143 WNT10A Wnt family member 10A 2 2
MIRT533540 TPR translocated promoter region, nuclear basket protein 2 2
MIRT533679 TMEM86A transmembrane protein 86A 2 2
MIRT540892 SRSF9 serine and arginine rich splicing factor 9 2 2
MIRT541329 G3BP1 G3BP stress granule assembly factor 1 2 2
MIRT541850 PLIN5 perilipin 5 2 2
MIRT551431 F2 coagulation factor II, thrombin 2 2
MIRT552105 PPP1R1A protein phosphatase 1 regulatory inhibitor subunit 1A 2 2
MIRT564912 YTHDF1 YTH N6-methyladenosine RNA binding protein 1 2 2
MIRT568605 ACVR2A activin A receptor type 2A 2 2
MIRT572604 PAPLN papilin, proteoglycan like sulfated glycoprotein 2 2
MIRT574234 DMRT2 doublesex and mab-3 related transcription factor 2 2 2
MIRT575688 Map1b microtubule-associated protein 1B 2 2
MIRT576643 Mill2 MHC I like leukocyte 2 1 1
MIRT609877 RAD54L2 RAD54 like 2 2 4
MIRT610057 MYBPC1 myosin binding protein C, slow type 2 2
MIRT610791 KLK2 kallikrein related peptidase 2 2 2
MIRT617175 GOSR2 golgi SNAP receptor complex member 2 2 2
MIRT617707 RUSC2 RUN and SH3 domain containing 2 2 2
MIRT620577 WBSCR27 methyltransferase like 27 2 4
MIRT622657 POU2F3 POU class 2 homeobox 3 2 4
MIRT624561 BDP1 B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB 2 2
MIRT634255 TIAL1 TIA1 cytotoxic granule associated RNA binding protein like 1 2 2
MIRT634677 GDE1 glycerophosphodiester phosphodiesterase 1 2 2
MIRT635254 FBXL20 F-box and leucine rich repeat protein 20 2 2
MIRT637081 SELPLG selectin P ligand 2 2
MIRT639021 AAK1 AP2 associated kinase 1 2 2
MIRT640396 ZNF785 zinc finger protein 785 2 2
MIRT642441 CLUAP1 clusterin associated protein 1 2 2
MIRT645666 ADK adenosine kinase 2 2
MIRT646083 MGST3 microsomal glutathione S-transferase 3 2 2
MIRT650513 UFM1 ubiquitin fold modifier 1 2 2
MIRT652474 TMEM181 transmembrane protein 181 2 2
MIRT652584 TIMM8A translocase of inner mitochondrial membrane 8A 2 2
MIRT654763 PRKAR2A protein kinase cAMP-dependent type II regulatory subunit alpha 2 2
MIRT655200 PHAX phosphorylated adaptor for RNA export 2 2
MIRT658353 FAM65B RHO family interacting cell polarization regulator 2 2 2
MIRT661820 PRPSAP1 phosphoribosyl pyrophosphate synthetase associated protein 1 2 2
MIRT662190 MEI1 meiotic double-stranded break formation protein 1 2 2
MIRT664375 CYB5A cytochrome b5 type A 2 2
MIRT665025 ELK1 ELK1, ETS transcription factor 2 2
MIRT666492 SBNO1 strawberry notch homolog 1 2 2
MIRT668480 EXOSC2 exosome component 2 2 2
MIRT682768 TMEM120B transmembrane protein 120B 2 2
MIRT689628 NAA30 N(alpha)-acetyltransferase 30, NatC catalytic subunit 2 2
MIRT691846 OSCAR osteoclast associated, immunoglobulin-like receptor 2 2
MIRT696490 COX6B1 cytochrome c oxidase subunit 6B1 2 2
MIRT712480 FSTL3 follistatin like 3 2 2
MIRT712780 ZNF154 zinc finger protein 154 2 2
MIRT716607 MPPED1 metallophosphoesterase domain containing 1 2 2
MIRT719357 ITPKB inositol-trisphosphate 3-kinase B 2 2
MIRT719739 SLC39A11 solute carrier family 39 member 11 2 2
MIRT722569 C1orf95 stum, mechanosensory transduction mediator homolog 2 2
MIRT722838 C17orf102 chromosome 17 open reading frame 102 2 2
MIRT733138 LINC00963 long intergenic non-protein coding RNA 963 3 0
MIRT733139 CHI3L1 chitinase 3 like 1 3 0
MIRT736944 TARBP2 TARBP2, RISC loading complex RNA binding subunit 2 0
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-378g Fluorouracil 3385 NSC19893 approved resistant High Pancreatic Cancer cell line (PANC-1)
hsa-miR-378g Gemcitabine 60750 NSC613327 approved resistant High Pancreatic Cancer cell line (PANC-1)
hsa-miR-378g Vemurafenib 42611257 NSC761431 approved sensitive High Melanoma cell line (A375, IGR37, 501Mel)
hsa-miR-378g Dabrafenib 44462760 NSC764134 approved sensitive High Melanoma cell line (A375, IGR37, 501Mel)
hsa-miR-378g Trametinib 11707110 NSC758246 approved resistant High Melanoma cell line (WM266) (1uM)
hsa-miR-378g Vemurafenib 42611257 NSC761431 approved resistant High Melanoma cell line (WM266) (1uM)
hsa-miR-378g Trametinib 11707110 NSC758246 approved resistant High Melanoma cell line (WM266) (500nM)
hsa-miR-378g Vemurafenib 42611257 NSC761431 approved resistant High Melanoma cell line (WM266) (500nM)
hsa-miR-378g Vemurafenib 42611257 NSC761431 approved resistant High Melanoma cell line (WM266) (2uM)
hsa-miR-378g Trametinib 11707110 NSC758246 approved resistant High Melanoma cell line (WM266) (2uM)
hsa-miR-378g Gemcitabine 60750 NSC613327 approved sensitive High Pancreatic Cancer cell line (AsPC-1)
hsa-miR-378g Trametinib 11707110 NSC758246 approved sensitive High Melanoma cell line
hsa-miR-378g Vemurafenib 42611257 NSC761431 approved sensitive High Melanoma cell line
hsa-miR-378g Dabrafenib + Trametinib sensitive High Melanoma cell line
hsa-miR-378g Dabrafenib 44462760 NSC764134 approved sensitive High Melanoma cell line
hsa-mir-378g Decitabine 451668 approved sensitive tissue (esopheageal cancer)
hsa-miR-378g Temozolomide 5394 NSC362856 approved resistant cell line (U251)
hsa-miR-378g Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-378g Osimertinib 71496458 NSC779217 approved sensitive cell line (PC9)
hsa-miR-378g Osimertinib 71496458 NSC779217 approved resistant cell line (HCC827)
hsa-miR-378g Tripterygium wilfordii Hook F resistant tissue
hsa-miR-378g Vemurafenib 42611257 NSC761431 approved sensitive cell line (LM17)
hsa-miR-378g Vemurafenib 42611257 NSC761431 approved sensitive cell line (LM36)
hsa-miR-378g Vemurafenib 42611257 NSC761431 approved sensitive cell line (LM16)
hsa-miR-378g Paclitaxel 36314 NSC125973 approved sensitive cell line (BAS)
hsa-miR-378g Doxorubicin 31703 NSC123127 approved sensitive cell line (BAS)
hsa-miR-378g Neoadjuvant chemotherapy resistant tissue (breast cancer)
hsa-miR-378g Gemcitabine 60750 NSC613327 approved resistant cell line (Panc1-GR3)
hsa-miR-378g Gemcitabine 60750 NSC613327 approved resistant cell line (Panc1-GR4)

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