>PRSS38|NM_183062|3'UTR
   1 GGTCAGGATACCCACTCTAGGATTCTCATGGCTGCACACCCTGCCCCAGCCCAGCTGCCTCCAGACCCCTAAGCATCTCC
  81 TGTCCTGGCCTCTCTGAAGCAGACAAGGGCCACCTATCCCGGGGGTGGATGCTGAGTCCAGGAGGTGATGAGCAAGTGTA
 161 CAAAAGAAAAAAGGGAAGGGGGAGAGGGGCTGGTCAGGGAGAACCCAGCTTGGGCAGAGTGCACCTGAGATTTGATAAGA
 241 TCATTAAATATTTACAAAGCAA

ID	Duplex structure	Position	Score	MFE
1	miRNA 3' gacacCCCCGUCCUCCCCGc 5' |||| | ||||||| Target 5' gggaaGGGGGA-GAGGGGCt 3'	173 - 191	154.00	-24.10
2	miRNA 3' gacACCCCCGUCCUCCCCgc 5' || | ||||||| | Target 5' tgcTGAGTCCAGGAGGTGat 3'	130 - 149	109.00	-13.70
3	miRNA 3' gacacccccguccUCCCCGc 5' | |||| Target 5' ctctgaagcagacAAGGGCc 3'	92 - 111	88.00	-11.10

Mutant ID	Mutant Position	Mutant Source
COSN31492613	12	COSMIC
COSN30119465	13	COSMIC
COSN30090251	15	COSMIC
COSN31547598	18	COSMIC
COSN8407124	18	COSMIC
COSN30120334	26	COSMIC
COSN30148228	44	COSMIC
COSN30449282	50	COSMIC
COSN510222	68	COSMIC
COSN26990580	71	COSMIC
COSN28574688	104	COSMIC
COSN31593113	110	COSMIC
COSN30474974	119	COSMIC
COSN30483840	120	COSMIC
COSN30497733	131	COSMIC
COSN30166598	135	COSMIC
COSN30183343	148	COSMIC
COSN30104814	161	COSMIC

Mutant ID	Mutant Position	Mutant Source
rs757854220	8	dbSNP
rs1331847793	13	dbSNP
rs1235737054	22	dbSNP
rs768128932	29	dbSNP
rs750888184	33	dbSNP
rs1209499584	36	dbSNP
rs756518307	38	dbSNP
rs1279420127	55	dbSNP
rs1272212881	59	dbSNP
rs1056615235	60	dbSNP
rs1466743531	71	dbSNP
rs887246999	74	dbSNP
rs1194973628	76	dbSNP
rs867478008	81	dbSNP
rs1301332343	84	dbSNP
rs943925443	86	dbSNP
rs552950535	90	dbSNP
rs3795766	104	dbSNP
rs1180342815	105	dbSNP
rs929764717	105	dbSNP
rs1406972900	113	dbSNP
rs1173005863	114	dbSNP
rs1048122555	115	dbSNP
rs888642110	119	dbSNP
rs1365890583	121	dbSNP
rs752852221	122	dbSNP
rs535236959	123	dbSNP
rs1488346886	125	dbSNP
rs553262941	126	dbSNP
rs1213224207	132	dbSNP
rs763781041	142	dbSNP
rs891193838	143	dbSNP
rs1014885824	155	dbSNP
rs1294218362	158	dbSNP
rs1391313302	159	dbSNP
rs1020323863	160	dbSNP
rs1304843212	162	dbSNP
rs1337293327	164	dbSNP
rs555789810	167	dbSNP
rs1281951618	168	dbSNP
rs1228845871	169	dbSNP
rs1362755491	171	dbSNP
rs568618736	179	dbSNP
rs567644393	180	dbSNP
rs535611195	185	dbSNP
rs185496995	187	dbSNP
rs530259753	189	dbSNP
rs967719343	191	dbSNP
rs757058636	193	dbSNP
rs985325364	195	dbSNP
rs753330958	212	dbSNP
rs1265488690	223	dbSNP
rs911015084	243	dbSNP
rs369216303	257	dbSNP
rs1421026500	261	dbSNP

- Genes & development, 2013

When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.