>SLC22A6|NM_153276|3'UTR
   1 GGACTGAGAAGGGGCCTTACAGAACCCTAAAGGGAGGGAAGGTCCTACAGGTCTCCGGCCACCCACACAAGGAGGAGGAA
  81 GAGGAAATGGTGACCCAAGTGTGGGGGTTGTGGTTCAGGAAAGCATCTTCCCAGGGGTCCACCTCCCTTTATAAACCCCA
 161 CCAGAACCACATCATTAAAAGGTTTGACTGCGCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

ID	Duplex structure	Position	Score	MFE
1	miRNA 3' ggUUCUCU-ACAACCGGGAUug 5' |||:|| | ||:|||| Target 5' taAAGGGAGGGAAGGTCCTAca 3'	28 - 49	123.00	-16.50
2	miRNA 3' gguuCUCUACAACCGGGAUug 5' |||| | ||||:|| Target 5' gactGAGAAG-GGGCCTTAca 3'	2 - 21	120.00	-13.00
3	miRNA 3' ggUUCUCUACAACCGGGAUUg 5' |:|| ||| | | || || Target 5' agAGGAAATGGT-GACCCAAg 3'	80 - 99	98.00	-9.40

Mutant ID	Mutant Position	Mutant Source
COSN30489334	1	COSMIC
COSN30482700	12	COSMIC
COSN31534387	22	COSMIC
COSN30511766	27	COSMIC
COSN30456780	33	COSMIC
COSN1135864	44	COSMIC
COSN30513344	47	COSMIC
COSN21567279	63	COSMIC
COSN30449859	84	COSMIC
COSN31492531	88	COSMIC
COSN31503418	92	COSMIC
COSN1135863	116	COSMIC
COSN31522193	156	COSMIC
COSN8687198	166	COSMIC
COSN14922951	191	COSMIC

Mutant ID	Mutant Position	Mutant Source
rs1159008442	4	dbSNP
rs367650942	10	dbSNP
rs781112424	11	dbSNP
rs756987056	14	dbSNP
rs751340783	15	dbSNP
rs374141912	20	dbSNP
rs1393834651	28	dbSNP
rs1246990664	32	dbSNP
rs1193014574	33	dbSNP
rs1206312079	34	dbSNP
rs1448167974	37	dbSNP
rs1325772838	38	dbSNP
rs150811286	46	dbSNP
rs376360109	47	dbSNP
rs758049448	49	dbSNP
rs1003538281	56	dbSNP
rs181212822	57	dbSNP
rs1230155903	60	dbSNP
rs558752459	66	dbSNP
rs1270670357	67	dbSNP
rs1333509251	72	dbSNP
rs1340160653	81	dbSNP
rs1213844307	83	dbSNP
rs1268981608	96	dbSNP
rs1435769312	97	dbSNP
rs1181474448	103	dbSNP
rs1233844544	123	dbSNP
rs1473817554	123	dbSNP
rs923506163	125	dbSNP
rs191546329	130	dbSNP
rs1417842737	136	dbSNP
rs1462004142	137	dbSNP
rs1041787229	156	dbSNP
rs945716192	161	dbSNP
rs185945462	172	dbSNP
rs1327863587	173	dbSNP
rs1350899355	178	dbSNP
rs1054132242	191	dbSNP
rs968175286	192	dbSNP

- Genes & development, 2013

When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.