pre-miRNA Information
pre-miRNA hsa-mir-6819   
Genomic Coordinates chr22: 36286847 - 36286907
Description Homo sapiens miR-6819 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-6819-3p
Sequence 41| AAGCCUCUGUCCCCACCCCAG |61
Evidence Experimental
Experiments Meta-analysis
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1279530967 2 dbSNP
rs967579979 3 dbSNP
rs776891214 4 dbSNP
rs771166635 8 dbSNP
rs747037324 9 dbSNP
rs879248090 11 dbSNP
rs1485325191 12 dbSNP
rs778157316 16 dbSNP
Putative Targets

Gene Information
Gene Symbol PSMB5   
Synonyms LMPX, MB1, X
Description proteasome subunit beta 5
Transcript NM_001144932   
Other Transcripts NM_001130725 , NM_002797   
Expression
Putative miRNA Targets on PSMB5
3'UTR of PSMB5
(miRNA target sites are highlighted)
>PSMB5|NM_001144932|3'UTR
   1 AGGGAACCGGATTTCAGGGGCCACCTTCTCTGTAGGTTCTGGCTCTGTGTATGCATATGGGGTCATGGATCGGGGCTATT
  81 CCTATGACCTGGAAGTGGAGCAGGCCTATGATCTGGCCCGTCGAGCCATCTACCAAGCCACCTACAGAGATGCCTACTCA
 161 GGAGGTGCAGTCAACCTCTACCACGTGCGGGAGGATGGCTGGATCCGAGTCTCCAGTGACAATGTGGCTGATCTACATGA
 241 GAAGTATAGTGGCTCTACCCCCTGAAAGAGGGTGGATGCAGCTGCTTGTGTTTCTTGGGGTGACTGTCATTGGTAATACG
 321 GACACAGTGACCCATCCTCCATCCTATTTATAGTGGAAGGGCCTTCAATTGTATCAGTACTTTTTTTTAAGCTCTGGCAC
 401 ATTGACCTCTATGTGTTACCAGTCATTAATGAGCTGCTGCAGAGGTGACTATTTGTTTTACTTTCTTGGATGTTAACATT
 481 ACACTACTCACTACTCAATCTCA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' gaCCCCA-CCCCUGUCUCCGAa 5'
            |||||   |||: |:|||| 
Target 5' atGGGGTCATGGATCGGGGCTa 3'
57 - 78 119.00 -22.70
2
miRNA  3' gaccccaccCCUGUCUCCGAa 5'
                   | |:|| |||| 
Target 5' acatgagaaGTATAGTGGCTc 3'
235 - 255 116.00 -7.60
3
miRNA  3' gaCCCCACCCCU---GUCUCCGaa 5'
            |||    |||   |||:|||  
Target 5' -aGGGAACCGGATTTCAGGGGCca 3'
1 - 23 115.00 -16.40
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSM954599 2 COSMIC
COSM6878687 15 COSMIC
COSM6757697 30 COSMIC
COSM1493206 46 COSMIC
COSM8944445 62 COSMIC
COSM8415692 64 COSMIC
COSM4501823 71 COSMIC
COSM6996000 95 COSMIC
COSM7623643 103 COSMIC
COSM4933016 108 COSMIC
COSM8647751 119 COSMIC
COSM4803677 120 COSMIC
COSM3744269 125 COSMIC
COSM3814515 140 COSMIC
COSM1733926 176 COSMIC
COSM8555187 185 COSMIC
COSM4049937 188 COSMIC
COSM8955599 189 COSMIC
COSM8996277 199 COSMIC
COSM6757694 201 COSMIC
COSM7118997 207 COSMIC
COSM7616155 211 COSMIC
COSM5711875 228 COSMIC
COSM1263255 243 COSMIC
COSM8540447 253 COSMIC
COSM246837 255 COSMIC
COSM9686638 260 COSMIC
COSM3711439 264 COSMIC
COSN1165877 302 COSMIC
COSN510243 319 COSMIC
COSN28846324 381 COSMIC
COSN19649868 391 COSMIC
COSN15660472 404 COSMIC
COSN31499535 424 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs773542531 8 dbSNP
rs372901909 9 dbSNP
rs1354011612 10 dbSNP
rs968890668 17 dbSNP
rs768336631 18 dbSNP
rs748576681 20 dbSNP
rs58556496 21 dbSNP
rs768368849 22 dbSNP
rs369321865 24 dbSNP
rs1173287716 27 dbSNP
rs779779692 28 dbSNP
rs758398552 32 dbSNP
rs750438167 35 dbSNP
rs1285060673 38 dbSNP
rs1480270975 43 dbSNP
rs778741331 45 dbSNP
rs1191849104 55 dbSNP
rs57148615 61 dbSNP
rs1483156871 65 dbSNP
rs753693697 69 dbSNP
rs767565432 71 dbSNP
rs1310796127 72 dbSNP
rs748037051 78 dbSNP
rs759817497 82 dbSNP
rs1220255471 84 dbSNP
rs751623199 88 dbSNP
rs12043 89 dbSNP
rs766491079 94 dbSNP
rs1411701796 95 dbSNP
rs763417612 106 dbSNP
rs60224002 108 dbSNP
rs765075694 112 dbSNP
rs1239499216 113 dbSNP
rs1440245650 115 dbSNP
rs1186208210 116 dbSNP
rs770354735 119 dbSNP
rs145689628 120 dbSNP
rs1418378625 123 dbSNP
rs760437419 127 dbSNP
rs140183720 132 dbSNP
rs1466102387 135 dbSNP
rs768191445 139 dbSNP
rs891067967 143 dbSNP
rs1256262527 150 dbSNP
rs1047397353 151 dbSNP
rs200785674 155 dbSNP
rs1198368373 173 dbSNP
rs746718225 174 dbSNP
rs140179061 176 dbSNP
rs373543398 178 dbSNP
rs749175846 180 dbSNP
rs1329039561 182 dbSNP
rs745405707 183 dbSNP
rs377390691 184 dbSNP
rs56688661 185 dbSNP
rs369529779 188 dbSNP
rs377286048 189 dbSNP
rs1220816063 193 dbSNP
rs930445021 196 dbSNP
rs1381643370 198 dbSNP
rs1303171893 199 dbSNP
rs1456481786 206 dbSNP
rs372740401 207 dbSNP
rs751817666 210 dbSNP
rs766617569 211 dbSNP
rs1344918727 213 dbSNP
rs1367315491 223 dbSNP
rs758424502 224 dbSNP
rs750529649 226 dbSNP
rs558713731 233 dbSNP
rs1038085901 237 dbSNP
rs1289832940 239 dbSNP
rs1256167071 243 dbSNP
rs202216566 244 dbSNP
rs764603824 246 dbSNP
rs760376551 247 dbSNP
rs1330441502 248 dbSNP
rs775245870 250 dbSNP
rs771877767 255 dbSNP
rs1323621911 257 dbSNP
rs773975356 261 dbSNP
rs146647954 262 dbSNP
rs927039184 263 dbSNP
rs981664806 271 dbSNP
rs761732182 273 dbSNP
rs113091841 278 dbSNP
rs367819967 284 dbSNP
rs1322303492 288 dbSNP
rs58302037 294 dbSNP
rs755861710 299 dbSNP
rs747958137 300 dbSNP
rs1458969631 302 dbSNP
rs1368597547 306 dbSNP
rs1167561619 308 dbSNP
rs1447284038 309 dbSNP
rs1270502071 319 dbSNP
rs2230087 320 dbSNP
rs190864003 326 dbSNP
rs1404385038 327 dbSNP
rs550599263 343 dbSNP
rs934667677 345 dbSNP
rs1341345503 346 dbSNP
rs924663593 348 dbSNP
rs1281699888 352 dbSNP
rs978851520 358 dbSNP
rs1276122932 359 dbSNP
rs530817136 362 dbSNP
rs1264224811 366 dbSNP
rs1023132814 368 dbSNP
rs974732294 371 dbSNP
rs992094808 375 dbSNP
rs1219381266 389 dbSNP
rs964818616 409 dbSNP
rs960299967 411 dbSNP
rs58831036 413 dbSNP
rs1036370432 421 dbSNP
rs1003131588 424 dbSNP
rs1025565265 425 dbSNP
rs994449316 437 dbSNP
rs950394600 440 dbSNP
rs1025979701 443 dbSNP
rs994543583 445 dbSNP
rs1437960304 449 dbSNP
rs1158117523 455 dbSNP
rs1426061544 456 dbSNP
rs778571314 461 dbSNP
rs1301699863 464 dbSNP
rs1390411842 467 dbSNP
rs1387675168 469 dbSNP
rs1452613906 480 dbSNP
rs1237414215 484 dbSNP
rs1252318850 486 dbSNP
rs1353993349 488 dbSNP
rs1263488707 490 dbSNP
rs144359924 492 dbSNP
rs1210192646 494 dbSNP
rs769957353 498 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084064. RNA binding protein: AGO2. Condition:CLIP_noemetine_AbnovaAb HITS-CLIP data was present in GSM1084065. RNA binding protein: AGO2. Condition:CLIP_emetine_AbnovaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' gaccccaCCCCUGUCUCCGAa 5'
                 ||| | :|||||| 
Target 5' -------GGGAAGGGAGGCUg 3'
1 - 14
Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084064
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_noemetine_AbnovaAb
Location of target site ENST00000493471.2 | 3UTR | GGAAGGGAGGCUGAGGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM1084065
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_emetine_AbnovaAb
Location of target site ENST00000493471.2 | 3UTR | GGGAAGGGAGGCUGAGGCU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
108 hsa-miR-6819-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT358318 PANK3 pantothenate kinase 3 2 2
MIRT454719 UBQLNL ubiquilin like 2 2
MIRT471362 PEG10 paternally expressed 10 2 2
MIRT478101 DLG5 discs large MAGUK scaffold protein 5 2 4
MIRT495554 GOLGA6L4 golgin A6 family-like 4 2 2
MIRT495587 GOLGA6L10 golgin A6 family-like 10 2 2
MIRT508315 RPL18 ribosomal protein L18 2 4
MIRT529183 ADAM33 ADAM metallopeptidase domain 33 2 2
MIRT532035 FHDC1 FH2 domain containing 1 2 2
MIRT545131 ANXA5 annexin A5 2 2
MIRT567288 HNRNPL heterogeneous nuclear ribonucleoprotein L 2 2
MIRT568094 CEBPG CCAAT/enhancer binding protein gamma 2 2
MIRT573291 DLX4 distal-less homeobox 4 2 2
MIRT575169 Fam120c family with sequence similarity 120, member C 2 2
MIRT609495 DNASE1L3 deoxyribonuclease 1 like 3 2 2
MIRT611264 EHD3 EH domain containing 3 2 2
MIRT613580 POC5 POC5 centriolar protein 2 2
MIRT616520 C9orf170 chromosome 9 open reading frame 170 2 2
MIRT617098 ZNF667 zinc finger protein 667 2 2
MIRT618268 DDX51 DEAD-box helicase 51 2 2
MIRT618294 ZNF682 zinc finger protein 682 2 2
MIRT618617 SHOX short stature homeobox 2 2
MIRT619741 SRFBP1 serum response factor binding protein 1 2 2
MIRT619784 NRIP2 nuclear receptor interacting protein 2 2 2
MIRT621119 SP110 SP110 nuclear body protein 2 2
MIRT622530 RAD51 RAD51 recombinase 2 2
MIRT623088 NME6 NME/NM23 nucleoside diphosphate kinase 6 2 2
MIRT623938 FKBP14 FK506 binding protein 14 2 2
MIRT624389 CD84 CD84 molecule 2 2
MIRT624860 ABI2 abl interactor 2 2 2
MIRT628752 TRPV2 transient receptor potential cation channel subfamily V member 2 2 2
MIRT632243 VTA1 vesicle trafficking 1 2 2
MIRT632987 E2F2 E2F transcription factor 2 2 2
MIRT633695 RBM43 RNA binding motif protein 43 2 2
MIRT633766 CENPBD1 CENPB DNA-binding domain containing 1 2 2
MIRT633808 ZNF91 zinc finger protein 91 2 2
MIRT638914 CBLN2 cerebellin 2 precursor 2 2
MIRT641298 SLAMF1 signaling lymphocytic activation molecule family member 1 2 2
MIRT642123 SRCAP Snf2 related CREBBP activator protein 2 2
MIRT642179 HEBP2 heme binding protein 2 2 2
MIRT642617 CDKN3 cyclin dependent kinase inhibitor 3 2 2
MIRT643325 TMEM151B transmembrane protein 151B 2 2
MIRT644534 TMEM134 transmembrane protein 134 2 2
MIRT645820 OMA1 OMA1 zinc metallopeptidase 2 2
MIRT646093 MGST3 microsomal glutathione S-transferase 3 2 2
MIRT646861 SLC35E4 solute carrier family 35 member E4 2 2
MIRT647466 DZIP1L DAZ interacting zinc finger protein 1 like 2 2
MIRT647702 NADSYN1 NAD synthetase 1 2 2
MIRT648082 ZMIZ2 zinc finger MIZ-type containing 2 2 2
MIRT648547 TMEM169 transmembrane protein 169 2 2
MIRT648690 AP1M1 adaptor related protein complex 1 mu 1 subunit 2 2
MIRT649747 GNB5 G protein subunit beta 5 2 2
MIRT650154 ZNF426 zinc finger protein 426 2 2
MIRT650450 CPXM2 carboxypeptidase X, M14 family member 2 2 2
MIRT650943 CTNS cystinosin, lysosomal cystine transporter 2 2
MIRT651544 WNK1 WNK lysine deficient protein kinase 1 2 2
MIRT652304 TNRC6B trinucleotide repeat containing 6B 2 2
MIRT652890 SYVN1 synoviolin 1 2 2
MIRT654222 RNF19B ring finger protein 19B 2 2
MIRT654676 PSMB5 proteasome subunit beta 5 2 2
MIRT658249 FAXC failed axon connections homolog 2 2
MIRT658363 FAM65B RHO family interacting cell polarization regulator 2 2 2
MIRT659622 CELF1 CUGBP Elav-like family member 1 2 2
MIRT660171 BNIP3L BCL2 interacting protein 3 like 2 2
MIRT660634 ANKRD52 ankyrin repeat domain 52 2 2
MIRT662428 TMCO1 transmembrane and coiled-coil domains 1 2 2
MIRT662984 PAK3 p21 (RAC1) activated kinase 3 2 2
MIRT663939 ZNF554 zinc finger protein 554 2 2
MIRT666960 PKHD1 PKHD1, fibrocystin/polyductin 2 2
MIRT667945 HNRNPC heterogeneous nuclear ribonucleoprotein C (C1/C2) 2 2
MIRT668847 CYCS cytochrome c, somatic 2 2
MIRT671792 RGS17 regulator of G protein signaling 17 2 2
MIRT672382 RPL37 ribosomal protein L37 2 2
MIRT673516 TNFAIP8L1 TNF alpha induced protein 8 like 1 2 4
MIRT674716 FAM73A mitoguardin 1 2 2
MIRT676352 KLF8 Kruppel like factor 8 2 2
MIRT677820 MRPS10 mitochondrial ribosomal protein S10 2 2
MIRT678440 PDE4C phosphodiesterase 4C 2 2
MIRT678499 ARHGEF39 Rho guanine nucleotide exchange factor 39 2 2
MIRT679243 LRP10 LDL receptor related protein 10 2 2
MIRT693607 SLC39A1 solute carrier family 39 member 1 2 2
MIRT695927 ZNF174 zinc finger protein 174 2 2
MIRT697987 TSPAN6 tetraspanin 6 2 2
MIRT700821 PHLDA2 pleckstrin homology like domain family A member 2 2 2
MIRT701350 NR4A3 nuclear receptor subfamily 4 group A member 3 2 2
MIRT702348 KLHL7 kelch like family member 7 2 2
MIRT703387 GABPB1 GA binding protein transcription factor beta subunit 1 2 2
MIRT703883 ERBB2IP erbb2 interacting protein 2 2
MIRT708237 PPP1R26 protein phosphatase 1 regulatory subunit 26 2 2
MIRT708629 NUDT18 nudix hydrolase 18 2 2
MIRT708990 CABP4 calcium binding protein 4 2 2
MIRT709804 FOXE1 forkhead box E1 2 2
MIRT710203 FLVCR1 feline leukemia virus subgroup C cellular receptor 1 2 2
MIRT710248 VAMP1 vesicle associated membrane protein 1 2 2
MIRT710281 FGF1 fibroblast growth factor 1 2 2
MIRT712992 IRGQ immunity related GTPase Q 2 2
MIRT713887 MOB3A MOB kinase activator 3A 2 2
MIRT715600 ADAM17 ADAM metallopeptidase domain 17 2 2
MIRT715639 ZBTB8B zinc finger and BTB domain containing 8B 2 2
MIRT715686 FRK fyn related Src family tyrosine kinase 2 2
MIRT717053 WIZ widely interspaced zinc finger motifs 2 2
MIRT717611 DSTYK dual serine/threonine and tyrosine protein kinase 2 2
MIRT721539 DKK3 dickkopf WNT signaling pathway inhibitor 3 2 2
MIRT722096 SUSD1 sushi domain containing 1 2 2
MIRT722435 HRNR hornerin 2 2
MIRT722855 NEGR1 neuronal growth regulator 1 2 2
MIRT724621 SNAP25 synaptosome associated protein 25 2 2
MIRT724777 PSG4 pregnancy specific beta-1-glycoprotein 4 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-6819 Ceritinib 57379345 NSC776422 approved resistant High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-miR-6819-3p Ceritinib 57379345 NSC776422 approved resistant High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-miR-6819-3p Cisplatin 5460033 NSC119875 approved resistant High Hypopharyngeal Cancer cell line (FaDu)
hsa-miR-6819-3p Gefitinib 123631 NSC715055 approved sensitive cell line (PC9)
hsa-miR-6819-3p Osimertinib 71496458 NSC779217 approved resistant cell line (HCC827)
hsa-miR-6819-3p Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-6819-3p Osimertinib 71496458 NSC779217 approved resistant cell line (H1975)
hsa-miR-6819-3p Cisplatin 5460033 NSC119875 approved resistant cell line (A2780)

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