pre-miRNA Information
pre-miRNA hsa-mir-6751   
Genomic Coordinates chr11: 65129916 - 65129978
Description Homo sapiens miR-6751 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-6751-3p
Sequence 43| ACUGAGCCUCUCUCUCUCCAG |63
Evidence Experimental
Experiments Meta-analysis
DRVs in miRNA
Mutant ID Mutant Position Mutant Source
COSM6795708 8 COSMIC
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs749989972 2 dbSNP
rs1266150108 3 dbSNP
rs1488089412 6 dbSNP
rs1259846893 8 dbSNP
rs3802937 10 dbSNP
rs371083440 12 dbSNP
rs1264909843 13 dbSNP
rs751042478 14 dbSNP
rs757551063 19 dbSNP
Putative Targets

Gene Information
Gene Symbol PFKM   
Synonyms ATP-PFK, GSD7, PFK-1, PFK1, PFKA, PFKX, PPP1R122
Description phosphofructokinase, muscle
Transcript NM_000289   
Other Transcripts NM_001166686 , NM_001166687 , NM_001166688   
Expression
Putative miRNA Targets on PFKM
3'UTR of PFKM
(miRNA target sites are highlighted)
>PFKM|NM_000289|3'UTR
   1 ACCTCTCTGGAGTGAGGGGAATAGATTACCTGATCATGGTCAGCTCACACCCTAATAAGTCCACATCTTCTCAGTGTTTT
  81 AGCTGTTTTTTTCATTAGGTTTCCTTTTATTCTGTACCTTGCAGCCATGACCAGTTCTGGCCAGGAGCTGGAGGAGCAGG
 161 CAGTGGGTGGGAGCTCCTTTTAGGTAGAATTTAACATGACTTCTGCCCCAGCTTTATCTGTCACACAAGGCTGGGCACCT
 241 CTAGTGCTACTGCTAGATATCACTTACTCAGTTAGAATTTTCCTAAAAATAAGCTTTATTTATTTCTTTGTGATAACAAA
 321 GAGTCTTGGTTCCTCTACTACTTTTACTACAGTGACAAATTGTAACTACACTAATAAATGCCAACTGGTCACTGTGCTTT
 401 TGCTTCTCCTGTTATCATCTTCCTAAGTGGAATGTAATACTGTCAGCCCCATGTATCAGACACTTGTCTGATGAAGCAGT
 481 AAAGACGTTAAGGGTATCACAGGGGGTGGAGGAAGGGATTATCTCTAGTACACTACTTGCTGGCTGTCTGAAAAATTGTC
 561 ACTGCCAAACTCTAAAAACAGTTCTAAATAGTGACTGAGAAGGTTTGTTGCTGGAGTCAGGGAATAAGGCAGCCAAATAC
 641 TCTTTGCACAGTTCTTTAGTGGGAAGAGAAATTAACAATAAATATCAAGCACTGTGAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' gaccucucucucucCGAGUca 5'
                        |||||  
Target 5' cctgatcatggtcaGCTCAca 3'
29 - 49 100.00 -6.30
2
miRNA  3' gaccucucucucuccGAGUCa 5'
                         ||||| 
Target 5' ataagtccacatcttCTCAGt 3'
55 - 75 100.00 -7.00
3
miRNA  3' gaccucucucucuccGAGUCa 5'
                         ||||| 
Target 5' gctagatatcacttaCTCAGt 3'
252 - 272 100.00 -7.50
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
308959 4 ClinVar
308960 99 ClinVar
308961 135 ClinVar
883071 164 ClinVar
883072 283 ClinVar
883073 412 ClinVar
883074 453 ClinVar
308962 490 ClinVar
308963 504 ClinVar
883843 533 ClinVar
883844 565 ClinVar
308964 623 ClinVar
883845 669 ClinVar
COSN7616338 68 COSMIC
COSN20109530 73 COSMIC
COSN7616339 73 COSMIC
COSN30119440 93 COSMIC
COSN30162152 94 COSMIC
COSN30154563 138 COSMIC
COSN31523265 200 COSMIC
COSN31520256 307 COSMIC
COSN31522056 395 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs367783282 4 dbSNP
rs1442004420 7 dbSNP
rs1302401897 9 dbSNP
rs1368448353 13 dbSNP
rs557362173 18 dbSNP
rs770576486 27 dbSNP
rs989052611 28 dbSNP
rs371922484 30 dbSNP
rs759302810 38 dbSNP
rs1291450160 40 dbSNP
rs765055747 44 dbSNP
rs752639418 49 dbSNP
rs1250766239 51 dbSNP
rs1452889896 52 dbSNP
rs190580764 55 dbSNP
rs1395958975 62 dbSNP
rs534563976 72 dbSNP
rs1269610425 84 dbSNP
rs945056955 85 dbSNP
rs562155917 86 dbSNP
rs1246709201 90 dbSNP
rs1408468034 93 dbSNP
rs370745910 95 dbSNP
rs1489813507 96 dbSNP
rs886049455 99 dbSNP
rs951703640 118 dbSNP
rs1377059291 121 dbSNP
rs1221423728 126 dbSNP
rs1262036560 130 dbSNP
rs886049456 135 dbSNP
rs1454769410 148 dbSNP
rs1254928314 154 dbSNP
rs1207088177 156 dbSNP
rs907614521 157 dbSNP
rs370250637 164 dbSNP
rs1207328437 166 dbSNP
rs1343293299 172 dbSNP
rs1802781 176 dbSNP
rs182762691 188 dbSNP
rs1372063800 191 dbSNP
rs917391721 197 dbSNP
rs1372582541 199 dbSNP
rs1294706725 205 dbSNP
rs950297924 206 dbSNP
rs943178164 208 dbSNP
rs1345488336 230 dbSNP
rs1319865132 239 dbSNP
rs1412558742 243 dbSNP
rs14683 254 dbSNP
rs149738911 258 dbSNP
rs1044648366 266 dbSNP
rs1455493745 266 dbSNP
rs748854817 270 dbSNP
rs534773615 271 dbSNP
rs1039276533 273 dbSNP
rs905889412 274 dbSNP
rs1475125780 279 dbSNP
rs8977 283 dbSNP
rs1195527608 285 dbSNP
rs1387432855 290 dbSNP
rs1054582893 295 dbSNP
rs994742923 298 dbSNP
rs1026258609 299 dbSNP
rs773679612 311 dbSNP
rs1317904847 318 dbSNP
rs886415081 318 dbSNP
rs1361967849 319 dbSNP
rs1383695740 320 dbSNP
rs3189641 322 dbSNP
rs1445302775 324 dbSNP
rs752194116 325 dbSNP
rs1317129194 333 dbSNP
rs1019213521 341 dbSNP
rs1333516008 341 dbSNP
rs1167874655 344 dbSNP
rs901668412 368 dbSNP
rs1420236661 383 dbSNP
rs1243828468 384 dbSNP
rs1261038995 385 dbSNP
rs756489697 395 dbSNP
rs111633950 398 dbSNP
rs79507126 412 dbSNP
rs1189145068 414 dbSNP
rs951755618 416 dbSNP
rs543335770 418 dbSNP
rs1204356558 425 dbSNP
rs1307600861 434 dbSNP
rs777702144 437 dbSNP
rs867495120 439 dbSNP
rs1203066637 440 dbSNP
rs1247003473 452 dbSNP
rs563335260 453 dbSNP
rs1293518669 454 dbSNP
rs1184860470 461 dbSNP
rs1416455418 462 dbSNP
rs1348337088 463 dbSNP
rs1307349229 466 dbSNP
rs1442787445 473 dbSNP
rs1355210422 474 dbSNP
rs1241855730 477 dbSNP
rs1014557535 480 dbSNP
rs1403647043 482 dbSNP
rs1417495291 487 dbSNP
rs962112960 488 dbSNP
rs886049457 490 dbSNP
rs991899762 495 dbSNP
rs1177156816 497 dbSNP
rs1444500160 498 dbSNP
rs1235069113 503 dbSNP
rs747797192 504 dbSNP
rs1365982302 506 dbSNP
rs1452066272 507 dbSNP
rs980382074 510 dbSNP
rs1488017373 530 dbSNP
rs1407076491 532 dbSNP
rs927525907 533 dbSNP
rs1224109241 534 dbSNP
rs1471017151 545 dbSNP
rs1334733220 557 dbSNP
rs957559622 566 dbSNP
rs1054879611 571 dbSNP
rs1380514716 575 dbSNP
rs1463163682 580 dbSNP
rs573511552 583 dbSNP
rs946148459 592 dbSNP
rs1307598071 613 dbSNP
rs532693406 617 dbSNP
rs568063197 623 dbSNP
rs1420041961 640 dbSNP
rs1410871467 642 dbSNP
rs777314667 643 dbSNP
rs1253041063 650 dbSNP
rs1215204061 653 dbSNP
rs987546386 655 dbSNP
rs911601099 658 dbSNP
rs1323739169 661 dbSNP
rs559151269 662 dbSNP
rs535423254 669 dbSNP
rs1205031690 674 dbSNP
rs1318491614 679 dbSNP
rs995963047 691 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084065. RNA binding protein: AGO2. Condition:CLIP_emetine_AbnovaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' gacCUCUCUCUCU-CCGaguca 5'
             |||:|| :|| |||     
Target 5' gaaGAGGGAAGGAUGGC----- 3'
2 - 18
Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084065
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_emetine_AbnovaAb
Location of target site ENST00000312352.7 | 3UTR | UGAAGAGGGAAGGAUGGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
77 hsa-miR-6751-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT130738 GATAD2B GATA zinc finger domain containing 2B 2 4
MIRT134924 CCND2 cyclin D2 2 2
MIRT273034 ZBTB18 zinc finger and BTB domain containing 18 2 2
MIRT276645 KPNA3 karyopherin subunit alpha 3 2 6
MIRT446531 OAS2 2'-5'-oligoadenylate synthetase 2 2 2
MIRT456268 TDRKH tudor and KH domain containing 2 12
MIRT494454 BTG2 BTG anti-proliferation factor 2 2 2
MIRT494965 USP46 ubiquitin specific peptidase 46 2 2
MIRT496689 KREMEN1 kringle containing transmembrane protein 1 2 2
MIRT496697 RGS11 regulator of G protein signaling 11 2 2
MIRT504376 IRF4 interferon regulatory factor 4 2 6
MIRT510980 PFN2 profilin 2 2 6
MIRT512548 MFN2 mitofusin 2 2 6
MIRT513639 TP53INP2 tumor protein p53 inducible nuclear protein 2 2 2
MIRT514016 CAMSAP1 calmodulin regulated spectrin associated protein 1 2 4
MIRT514074 MTRNR2L6 MT-RNR2-like 6 2 2
MIRT515300 C15orf38-AP3S2 C15orf38-AP3S2 readthrough 2 4
MIRT517285 AP3S2 adaptor related protein complex 3 sigma 2 subunit 2 4
MIRT519075 KCNK6 potassium two pore domain channel subfamily K member 6 2 2
MIRT520779 TCF23 transcription factor 23 2 2
MIRT522485 MFSD9 major facilitator superfamily domain containing 9 2 2
MIRT528856 PKP1 plakophilin 1 2 2
MIRT529081 PATE2 prostate and testis expressed 2 2 2
MIRT531209 PLA2G4D phospholipase A2 group IVD 2 2
MIRT533988 TAB3 TGF-beta activated kinase 1 and MAP3K7 binding protein 3 2 2
MIRT537024 GRIN2B glutamate ionotropic receptor NMDA type subunit 2B 2 2
MIRT537498 FAM168B family with sequence similarity 168 member B 2 2
MIRT553125 UBE2Z ubiquitin conjugating enzyme E2 Z 2 2
MIRT555591 PIP5K1C phosphatidylinositol-4-phosphate 5-kinase type 1 gamma 2 2
MIRT556370 LUZP1 leucine zipper protein 1 2 2
MIRT569745 C2orf71 chromosome 2 open reading frame 71 2 2
MIRT570149 DNAJC10 DnaJ heat shock protein family (Hsp40) member C10 2 2
MIRT571243 FADS6 fatty acid desaturase 6 2 2
MIRT573065 TRIB1 tribbles pseudokinase 1 2 2
MIRT575533 Map4 microtubule-associated protein 4 2 2
MIRT575777 Tnfrsf10b tumor necrosis factor receptor superfamily, member 10b 2 2
MIRT616558 ZNF512B zinc finger protein 512B 2 2
MIRT624851 ABI2 abl interactor 2 2 2
MIRT630078 GRWD1 glutamate rich WD repeat containing 1 2 2
MIRT631479 KLHL21 kelch like family member 21 2 2
MIRT632173 CCL22 C-C motif chemokine ligand 22 2 2
MIRT638397 QSOX2 quiescin sulfhydryl oxidase 2 2 4
MIRT641192 ISG20L2 interferon stimulated exonuclease gene 20 like 2 2 2
MIRT642562 TEX9 testis expressed 9 2 2
MIRT642935 KRTAP5-9 keratin associated protein 5-9 2 2
MIRT649703 ZNF175 zinc finger protein 175 2 2
MIRT650437 CPXM2 carboxypeptidase X, M14 family member 2 2 2
MIRT652113 TRUB2 TruB pseudouridine synthase family member 2 2 2
MIRT652273 TOMM20 translocase of outer mitochondrial membrane 20 2 2
MIRT655215 PFKM phosphofructokinase, muscle 2 2
MIRT682778 ZNF852 zinc finger protein 852 2 2
MIRT683001 MUC20 mucin 20, cell surface associated 2 2
MIRT684320 GTF3C4 general transcription factor IIIC subunit 4 2 2
MIRT689435 CYB561 cytochrome b561 2 2
MIRT693847 ZNF107 zinc finger protein 107 2 2
MIRT696715 TAX1BP3 Tax1 binding protein 3 2 2
MIRT698597 TEX261 testis expressed 261 2 2
MIRT699973 RREB1 ras responsive element binding protein 1 2 2
MIRT703310 GFPT1 glutamine--fructose-6-phosphate transaminase 1 2 2
MIRT706796 RAI1 retinoic acid induced 1 2 2
MIRT709004 CD109 CD109 molecule 2 2
MIRT709181 TBC1D10B TBC1 domain family member 10B 2 2
MIRT709835 PAQR7 progestin and adipoQ receptor family member 7 2 2
MIRT711627 CORO1C coronin 1C 2 2
MIRT712634 RNF103-CHMP3 RNF103-CHMP3 readthrough 2 2
MIRT713694 CYB5R4 cytochrome b5 reductase 4 2 2
MIRT713752 SLC9A8 solute carrier family 9 member A8 2 2
MIRT714908 CHMP3 charged multivesicular body protein 3 2 2
MIRT716371 CBLL1 Cbl proto-oncogene like 1 2 2
MIRT718303 XPOT exportin for tRNA 2 2
MIRT718713 ANKRD18A ankyrin repeat domain 18A 2 2
MIRT718740 ATP9A ATPase phospholipid transporting 9A (putative) 2 2
MIRT719441 NPTX2 neuronal pentraxin 2 2 2
MIRT720896 OTUD4 OTU deubiquitinase 4 2 2
MIRT722448 RXFP4 relaxin/insulin like family peptide receptor 4 2 2
MIRT723882 VKORC1 vitamin K epoxide reductase complex subunit 1 2 2
MIRT724586 SYNJ2BP synaptojanin 2 binding protein 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-6751 Cisplatin 5460033 NSC119875 approved sensitive Low Ovarian Cancer cell line (A2780)
hsa-mir-6751 Triptolide 107985 NSC163062 sensitive Low Ovarian Cancer cell line (A2780)

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