pre-miRNA Information
pre-miRNA hsa-mir-4301   
Genomic Coordinates chr11: 113450023 - 113450088
Description Homo sapiens miR-4301 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4301
Sequence 11| UCCCACUACUUCACUUGUGA |30
Evidence Experimental
Experiments SOLiD
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs184176277 2 dbSNP
rs1191324913 13 dbSNP
rs1369198005 15 dbSNP
rs1036973189 16 dbSNP
rs944900541 18 dbSNP
Putative Targets

miRNA Expression profile
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol ETV3   
Synonyms METS, PE-1, PE1
Description ETS variant 3
Transcript NM_005240   
Other Transcripts NM_001145312   
Expression
Putative miRNA Targets on ETV3
3'UTR of ETV3
(miRNA target sites are highlighted)
>ETV3|NM_005240|3'UTR
   1 TTTTGAATTGAAAAGAATTTTTAAAAATCCAAATCTAAGACATGGCATGTTTAGGAAGATTTTAGAAACACTAAAATAAT
  81 GTGATCCTTTGGATTGCCTCAATGTTCTTACTCAAGTCATCTCACTTATAAGGAGAGTTATAGGCTATTCAGTATCAAGA
 161 TAGATTTCTTTGGTTTATTTGGTTGGTTCCCTTTTCTGCATATTGTTTGTAATCTCCTAGATACTATTACGCTATCTTGT
 241 TTGGGAATGATGTTTCATAGGTTTGTGATGATCTTTACGTTCAGGACTCAGTTTTAACACCCAGCCCAGTGGTTCTTTCA
 321 TAGATGGGAACCTGTTTCTACAAACACTTCCGATTTTCTGTGAAACTACCAAGCTCTCCCTTATCAAGTGAATATCATCA
 401 AAACCACAGCATCCTTGATCAGAGAAGGGGGAGGTTCACATGTTTGCAGTGAAAAGCAGTGTCTTTGATCTGCAACAGCA
 481 AATCCTCAGAGAAAAAGATTCTGGGGTTACTTGACCTTCTCTCCTGTTAAGTGCAGTAGGGCTTCCCCTCTTGACTTTCC
 561 TGGTTATAGCTTTCCATCACAGCTCCCCACATTCTCTCTTGATGTTGAAAGCAGTCTCTCAAAAGACTTTGTTGTTGTGT
 641 GGTTTTTTGTTTGTGATTTTTTTCCTTATGCAAATCATACTCCTGCCCAAGAAAATACAGTAGTTCCCCTTATCTGAGCA
 721 GTATATGTTCTAAGACCCCTAGTAGATTCGCAAACCACAGATAGTACCAAACTCCATTCATATATATGATGTTTTTTCTT
 801 CCCTTAACCCCACTCATATGTATCTGTGATAACGTTTAATTTATAAATTAGGCACAGTAAGAGATTAATGACAATAATAA
 881 AATAGAAAAATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' aguguucacuucAUC-ACCCu 5'
                      ||| |||| 
Target 5' gtggttctttcaTAGATGGGa 3'
309 - 329 108.00 -8.00
2
miRNA  3' aguGUUCACUUCAUCACccu 5'
             :||||| ||||| |   
Target 5' tgtTAAGTGCAGTAGGGctt 3'
525 - 544 101.00 -12.70
3
miRNA  3' aguguUCAC--UUCAUCACccu 5'
               ||||  ||| ||||   
Target 5' tttgcAGTGAAAAGCAGTGtct 3'
443 - 464 93.00 -7.22
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30147944 10 COSMIC
COSN30497524 10 COSMIC
COSN30518853 10 COSMIC
COSN30129441 15 COSMIC
COSN30520486 15 COSMIC
COSN31566767 26 COSMIC
COSN22776619 35 COSMIC
COSN26975461 35 COSMIC
COSN30107700 36 COSMIC
COSN31597653 40 COSMIC
COSN31531582 101 COSMIC
COSN28836383 145 COSMIC
COSN30111948 168 COSMIC
COSN31553787 231 COSMIC
COSN21413848 252 COSMIC
COSN31523609 458 COSMIC
COSN8320411 459 COSMIC
COSN28725145 521 COSMIC
COSN31536305 586 COSMIC
COSN22399394 867 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs778593027 9 dbSNP
rs888666050 15 dbSNP
rs756900055 17 dbSNP
rs748939441 18 dbSNP
rs778189711 20 dbSNP
rs756506856 33 dbSNP
rs1370585983 34 dbSNP
rs1312701249 36 dbSNP
rs1436980959 37 dbSNP
rs1311394573 38 dbSNP
rs1362649136 41 dbSNP
rs753068761 46 dbSNP
rs767954954 47 dbSNP
rs1351064729 48 dbSNP
rs528882206 51 dbSNP
rs996832546 52 dbSNP
rs1289583857 58 dbSNP
rs561315293 73 dbSNP
rs1394561218 86 dbSNP
rs1336352287 92 dbSNP
rs113772942 93 dbSNP
rs531889229 101 dbSNP
rs201311526 102 dbSNP
rs1020426630 107 dbSNP
rs913733198 121 dbSNP
rs374703320 129 dbSNP
rs936596015 134 dbSNP
rs1161000927 135 dbSNP
rs1457067296 138 dbSNP
rs1385548905 140 dbSNP
rs189182662 154 dbSNP
rs1053449288 159 dbSNP
rs1448051862 166 dbSNP
rs757088145 171 dbSNP
rs899499456 173 dbSNP
rs546072888 176 dbSNP
rs1194548011 185 dbSNP
rs1041116552 186 dbSNP
rs917685087 189 dbSNP
rs943929609 190 dbSNP
rs1160446031 204 dbSNP
rs1265325165 208 dbSNP
rs971839036 212 dbSNP
rs1174543845 213 dbSNP
rs1376232861 217 dbSNP
rs961573005 223 dbSNP
rs572561624 224 dbSNP
rs1307256336 227 dbSNP
rs1348361719 228 dbSNP
rs908473705 230 dbSNP
rs1360426290 231 dbSNP
rs1005682711 237 dbSNP
rs1294230828 240 dbSNP
rs1247809841 243 dbSNP
rs1046866054 246 dbSNP
rs565470316 248 dbSNP
rs1484215639 250 dbSNP
rs1178320657 251 dbSNP
rs931128486 267 dbSNP
rs1267198724 269 dbSNP
rs1431527771 271 dbSNP
rs1431361046 278 dbSNP
rs997281674 279 dbSNP
rs919782489 283 dbSNP
rs1429394519 285 dbSNP
rs758076545 291 dbSNP
rs1368505770 303 dbSNP
rs901155328 312 dbSNP
rs1041563949 316 dbSNP
rs1321005843 321 dbSNP
rs1009644969 325 dbSNP
rs1384999431 326 dbSNP
rs560588550 328 dbSNP
rs1371058815 344 dbSNP
rs1385788101 348 dbSNP
rs1365741007 350 dbSNP
rs1053570088 351 dbSNP
rs1265213337 352 dbSNP
rs542307545 352 dbSNP
rs1487831249 368 dbSNP
rs1216064264 369 dbSNP
rs958813695 371 dbSNP
rs928587675 373 dbSNP
rs981854737 380 dbSNP
rs1168899343 390 dbSNP
rs1392194138 409 dbSNP
rs1044972511 410 dbSNP
rs574920403 413 dbSNP
rs949114274 417 dbSNP
rs147487431 418 dbSNP
rs967624912 427 dbSNP
rs1327776641 428 dbSNP
rs762722298 430 dbSNP
rs1394624803 432 dbSNP
rs1476673608 432 dbSNP
rs1376496464 433 dbSNP
rs1433627993 434 dbSNP
rs1294969503 439 dbSNP
rs569821037 440 dbSNP
rs917584635 441 dbSNP
rs1198183751 456 dbSNP
rs1312513107 461 dbSNP
rs1320270663 463 dbSNP
rs372751602 470 dbSNP
rs765494727 476 dbSNP
rs1274279483 480 dbSNP
rs1271262032 484 dbSNP
rs760001918 492 dbSNP
rs1243914847 494 dbSNP
rs1486336967 496 dbSNP
rs1482878038 497 dbSNP
rs957530083 506 dbSNP
rs1412825024 509 dbSNP
rs1029149752 514 dbSNP
rs1271983273 521 dbSNP
rs996516855 526 dbSNP
rs899594319 527 dbSNP
rs576246266 545 dbSNP
rs1456630163 547 dbSNP
rs1318034337 548 dbSNP
rs1346423407 551 dbSNP
rs1231839528 552 dbSNP
rs1040690608 561 dbSNP
rs1301850568 569 dbSNP
rs1377438481 582 dbSNP
rs1008321980 592 dbSNP
rs886808800 593 dbSNP
rs1329148074 604 dbSNP
rs1227058509 607 dbSNP
rs1407585386 607 dbSNP
rs367728346 607 dbSNP
rs1029016067 610 dbSNP
rs1209717999 621 dbSNP
rs557989502 623 dbSNP
rs1235882286 631 dbSNP
rs74116988 634 dbSNP
rs965792967 639 dbSNP
rs1019619682 644 dbSNP
rs3199902 649 dbSNP
rs1009501493 663 dbSNP
rs140715351 664 dbSNP
rs1409557870 664 dbSNP
rs1174931198 667 dbSNP
rs1412015425 669 dbSNP
rs556194916 679 dbSNP
rs1416238547 680 dbSNP
rs1400606574 681 dbSNP
rs1055645608 682 dbSNP
rs1408555928 692 dbSNP
rs1281738418 696 dbSNP
rs1351496353 699 dbSNP
rs1000678395 706 dbSNP
rs1480775374 709 dbSNP
rs1431511269 711 dbSNP
rs1180154776 719 dbSNP
rs754293025 720 dbSNP
rs547108246 723 dbSNP
rs1280842742 727 dbSNP
rs1204884666 741 dbSNP
rs184869094 741 dbSNP
rs1045423434 744 dbSNP
rs192368853 749 dbSNP
rs928722412 750 dbSNP
rs949340613 758 dbSNP
rs150786870 759 dbSNP
rs751313665 766 dbSNP
rs1190197112 768 dbSNP
rs539685895 780 dbSNP
rs187552738 786 dbSNP
rs564635555 790 dbSNP
rs137889851 792 dbSNP
rs1408503218 794 dbSNP
rs1331479004 795 dbSNP
rs1324243356 798 dbSNP
rs1436389647 801 dbSNP
rs913269363 806 dbSNP
rs908988919 815 dbSNP
rs984518407 816 dbSNP
rs1297093736 818 dbSNP
rs1341204346 820 dbSNP
rs1193916387 828 dbSNP
rs760618234 833 dbSNP
rs757930867 834 dbSNP
rs1372092564 841 dbSNP
rs921434843 844 dbSNP
rs1250620032 846 dbSNP
rs1401593463 847 dbSNP
rs957624956 852 dbSNP
rs1444760170 863 dbSNP
rs1193881802 864 dbSNP
rs1339095869 872 dbSNP
rs1333976618 881 dbSNP
rs1474216525 882 dbSNP
rs1470570062 884 dbSNP
rs1029077776 886 dbSNP
rs1170077937 891 dbSNP
rs965676825 893 dbSNP
rs1405345143 899 dbSNP
rs1324410073 917 dbSNP
rs1396824962 919 dbSNP
rs1441643961 925 dbSNP
rs1319954448 930 dbSNP
rs7521824 933 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084064. RNA binding protein: AGO2. Condition:CLIP_noemetine_AbnovaAb HITS-CLIP data was present in GSM1084065. RNA binding protein: AGO2. Condition:CLIP_emetine_AbnovaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' aguguucacuucaUCACCCu 5'
                       |||||| 
Target 5' --------aaaagAGUGGGa 3'
1 - 12
Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084064
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_noemetine_AbnovaAb
Location of target site ENST00000368192.4 | 3UTR | AAAAGAGUGGGAGAUAGUUU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM1084065
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_emetine_AbnovaAb
Location of target site ENST00000368192.4 | 3UTR | AAAAGAGUGGGAGAUAGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
83 hsa-miR-4301 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT081134 LDLR low density lipoprotein receptor 2 4
MIRT090911 ARHGEF26 Rho guanine nucleotide exchange factor 26 2 2
MIRT229436 MECP2 methyl-CpG binding protein 2 2 2
MIRT274717 CPSF6 cleavage and polyadenylation specific factor 6 2 2
MIRT350957 BACH1 BTB domain and CNC homolog 1 2 2
MIRT386722 NUFIP2 NUFIP2, FMR1 interacting protein 2 2 2
MIRT446022 PEX3 peroxisomal biogenesis factor 3 2 2
MIRT481303 ATP5G3 ATP synthase, H+ transporting, mitochondrial Fo complex subunit C3 (subunit 9) 2 14
MIRT483959 ZNF354B zinc finger protein 354B 2 6
MIRT495667 TUBAL3 tubulin alpha like 3 2 2
MIRT496554 TBX15 T-box 15 2 2
MIRT497702 ARL6IP6 ADP ribosylation factor like GTPase 6 interacting protein 6 2 2
MIRT498105 RMND5A required for meiotic nuclear division 5 homolog A 2 2
MIRT512535 SEMA4D semaphorin 4D 2 2
MIRT512556 MFN2 mitofusin 2 2 6
MIRT521465 RABGAP1 RAB GTPase activating protein 1 2 6
MIRT526236 C2orf15 chromosome 2 open reading frame 15 2 2
MIRT526743 HLA-DOB major histocompatibility complex, class II, DO beta 2 2
MIRT527578 BRD7 bromodomain containing 7 2 4
MIRT528038 WT1 Wilms tumor 1 2 2
MIRT528267 GPRIN2 G protein regulated inducer of neurite outgrowth 2 2 2
MIRT528505 HTR7 5-hydroxytryptamine receptor 7 2 4
MIRT528758 RPS27 ribosomal protein S27 2 6
MIRT528999 IPO9 importin 9 2 2
MIRT529701 MRPL30 mitochondrial ribosomal protein L30 2 2
MIRT529781 C17orf82 chromosome 17 open reading frame 82 2 2
MIRT533797 TMEM119 transmembrane protein 119 2 6
MIRT535308 PHF12 PHD finger protein 12 2 2
MIRT535917 MKL2 MKL1/myocardin like 2 2 2
MIRT544892 OSBPL1A oxysterol binding protein like 1A 2 2
MIRT555423 PPIC peptidylprolyl isomerase C 2 2
MIRT562068 KLHL15 kelch like family member 15 2 2
MIRT565623 SLC31A1 solute carrier family 31 member 1 2 2
MIRT565661 SIX1 SIX homeobox 1 2 2
MIRT570138 IL1RL2 interleukin 1 receptor like 2 2 2
MIRT571045 YRDC yrdC N6-threonylcarbamoyltransferase domain containing 2 2
MIRT621232 LMAN1 lectin, mannose binding 1 2 2
MIRT622272 SH3TC2 SH3 domain and tetratricopeptide repeats 2 2 2
MIRT623844 GAN gigaxonin 2 2
MIRT626158 NFYA nuclear transcription factor Y subunit alpha 2 2
MIRT626464 CMKLR1 chemerin chemokine-like receptor 1 2 2
MIRT632087 ALDH1A2 aldehyde dehydrogenase 1 family member A2 2 2
MIRT637243 FAM26E calcium homeostasis modulator family member 5 2 2
MIRT642710 FGFR1OP2 FGFR1 oncogene partner 2 2 2
MIRT643611 KANSL3 KAT8 regulatory NSL complex subunit 3 2 2
MIRT644270 PAFAH1B1 platelet activating factor acetylhydrolase 1b regulatory subunit 1 2 2
MIRT645470 SPIN3 spindlin family member 3 2 2
MIRT649193 DNPEP aspartyl aminopeptidase 2 2
MIRT650776 POP4 POP4 homolog, ribonuclease P/MRP subunit 2 2
MIRT651345 ZC2HC1C zinc finger C2HC-type containing 1C 2 2
MIRT652237 TRAPPC3L trafficking protein particle complex 3 like 2 2
MIRT652587 TIMM8A translocase of inner mitochondrial membrane 8A 2 2
MIRT654591 PURA purine rich element binding protein A 2 2
MIRT654649 PTAFR platelet activating factor receptor 2 2
MIRT656842 KLF7 Kruppel like factor 7 2 2
MIRT657209 IKZF2 IKAROS family zinc finger 2 2 2
MIRT658513 ETV3 ETS variant 3 2 2
MIRT659448 CNNM2 cyclin and CBS domain divalent metal cation transport mediator 2 2 2
MIRT668816 CYLD CYLD lysine 63 deubiquitinase 2 2
MIRT669067 CELSR3 cadherin EGF LAG seven-pass G-type receptor 3 2 2
MIRT677711 ELOF1 elongation factor 1 homolog 2 4
MIRT687146 PTPN12 protein tyrosine phosphatase, non-receptor type 12 2 2
MIRT698498 THOC2 THO complex 2 2 2
MIRT707930 PPP1R3D protein phosphatase 1 regulatory subunit 3D 4 2
MIRT708737 FAM71F2 family with sequence similarity 71 member F2 2 2
MIRT710031 POLL DNA polymerase lambda 2 2
MIRT712175 STK4 serine/threonine kinase 4 2 2
MIRT715297 MAPK1 mitogen-activated protein kinase 1 2 2
MIRT716892 AGPAT6 glycerol-3-phosphate acyltransferase 4 2 2
MIRT717378 RBM41 RNA binding motif protein 41 2 2
MIRT718082 CLIC5 chloride intracellular channel 5 2 2
MIRT718721 ANKRD18A ankyrin repeat domain 18A 2 2
MIRT719152 DPYSL5 dihydropyrimidinase like 5 2 2
MIRT719224 CAMK4 calcium/calmodulin dependent protein kinase IV 2 2
MIRT719528 SRCIN1 SRC kinase signaling inhibitor 1 2 2
MIRT719861 KLF2 Kruppel like factor 2 2 2
MIRT720326 CAMK2G calcium/calmodulin dependent protein kinase II gamma 2 2
MIRT721596 SREBF1 sterol regulatory element binding transcription factor 1 2 2
MIRT722020 NEBL nebulette 2 2
MIRT722484 QSOX1 quiescin sulfhydryl oxidase 1 2 2
MIRT722614 TEAD1 TEA domain transcription factor 1 2 2
MIRT723664 RPTN repetin 2 2
MIRT723939 SVOP SV2 related protein 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-4 Dexamethasone approved 5743 Microarray primary rat thymocytes 20847043 2010 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-4301 Cisplatin 5460033 NSC119875 approved sensitive High Hepatocellular Carcinoma cell line (Hep3B, 97L)
hsa-miR-4301 Imatinib 5291 NSC743414 approved resistant High Chronic Myelogenous Leukemia cell line (K562)
hsa-mir-4301 Paclitaxel 36314 NSC125973 approved resistant cell line (BAS)
hsa-mir-4301 Paclitaxel 36314 NSC125973 approved sensitive cell line (W1)
hsa-mir-4301 Doxorubicin 31703 NSC123127 approved sensitive cell line (W1)
hsa-mir-4301 Methotrexate 126941 NSC740 approved sensitive cell line (W1)
hsa-mir-4301 Topotecan 60699 NSC609699 approved sensitive cell line (W1)
hsa-miR-4301 Gefitinib 123631 NSC715055 approved resistant cell line (PC9)
hsa-miR-4301 Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)

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