miRTarBase - #MIRT658754


—
pre-miRNA Information
pre-miRNA hsa-mir-4281   
Genomic Coordinates chr5: 176629439 - 176629500
Description Homo sapiens miR-4281 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4281
Sequence 35| GGGUCCCGGGGAGGGGGG |52
Evidence Experimental
Experiments SOLiD
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs921488943 1 dbSNP
rs1040286402 3 dbSNP
rs1375541904 8 dbSNP
rs759166911 12 dbSNP
rs768735686 13 dbSNP
rs746495416 14 dbSNP
rs779751131 18 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol ELAVL3   
Synonyms HUC, HUCL, PLE21
Description ELAV like RNA binding protein 3
Transcript NM_001420   
Other Transcripts NM_032281   
Expression
Putative miRNA Targets on ELAVL3
3'UTR of ELAVL3
(miRNA target sites are highlighted)		 
>ELAVL3|NM_001420|3'UTR
   1 GCCCACCCCGCCTGCCCTCCCACCCCCTCCCCGGGCAGCAGAGAGAGAGAGAGAGAAAGAGAGAGAGAGAGAGAGAAGGG
  81 GCCCAAGAGAGACAGCACAGGCAGCCCCACGGACGACGCGAGGGCCCCACGTCCCTGCGGAAGCCACAGGGTGAGCACTC
 161 TGGGGTGGGAGGGTCTGCAGGGAATTGGGGGGGTGCCCGGGGATCCCCCGCCCCATCCTCCTGCCCCCACCCCAGGCTGG
 241 GCTGTTCACTCTCTCGTCTTGGTTTGGTTCATGGTGAAGGTTTTTGTTTCTTTTTTCGGCTAAAAAGAATGCAGAGATGT
 321 GCCCCCACCCCCACCCTCGACCACCCCCGATGGGATGGCTTGGGGGGCTCCAGGGGGTGCCCTCCCAGACCCCCTTGCCC
 401 AGGCCTCCCCAGCACCTAGGTGGGGCCTGGGGTAGGAGGAACAGGTTTAAAAATCCCCAAAAAAGCGAACCGTGAGGAGG
 481 GGTGTGGGCACCCCCGGCCCAGTGCCCCCTGGTGGAATGCGGGGGAGCAGGCAGTGGGGCTGGAAGCAGAAACAAAATGA
 561 AAAAAAAAGGGGGGTGGGAGGGGAAGAAAAACTCTATTTTTGTAAAAAGGGAAAAAGACCTCGTGGAGAATTTTTACTGG
 641 GGATTCTTGAACTTGAAAAAAAAAAACACAAAAAAAGACAAAAAAAAAAAAAGAAAATATTTTGGCAGGTTATGTTTACC
 721 AACTGGGGCGGGGGTGGGGGGGGCCCAGGGAGCAGGGCTTAGGGGCTAGCAGCCCACGGGGCCACACAGAGAAACAACCA
 801 CGCAGACAGTCACACCACGGGGACACACGGACAGACGCAGACGGACACAGCGACATGCCCCAGACACGTTAAGGGACTGG
 881 TTGGCCAAACTCAGACACGTGGACAGGGATAGACAGAAAGAGACAGAAGCTGGGGCTACGTCCATGTGGACACAGACCAC
 961 AGATGTGGGCACACGAATCCATCCACCTGTCCACGTGCACACGTGAATGTAGCGATAGATATTTGGACATCAAATTTGGA
1041 CACCAGGTCACGGAGAAACACAAACACCACCCAGGAACACGCAGACATATGCCCATAGCATCCCACAGGCCCAGGCAGGA
1121 GGTCCCACCCCAGACCCTGCCCCAGACGCTCCCTCACCCTCTCGGCCCCCTCGCCCTGGCCCCCAGGTTCTCTGCAGAGA
1201 TCTCTCCTGGACCCCCAGCGGTCTCCTTGGCGCCCACGAACACAGGCGTGCACACGCAGCGCACATGCATTGAGACACAC
1281 GATACCTCGTTCCACCTTGGCGTCACGGTGGGCTGGAGGCAGCCCCCCCCCCCACAACACTTGAGGCCAAAGGACACCCC
1361 TGTCCCCGGGGCTCAGCCTCCCCCTGGGCCGAGGCCTTGGCCCACACAGCTGAGGGAGAGGCCCAGCCCTGAGCCTCCCC
1441 CACCAGGACTGGGCCTCCCCGGCAGCAAGACCCCGGCACTCCCCGCCAGGCCCAGGGTGGGGTGGATGGGCCCAAAGGCC
1521 CAGCCCCACACTCCTTTCCCGTCCACTTGTCACCTACCTATCCCCTTTCGGTTTGTTGGGTTTTTCGTCTTTCCAGATTG
1601 CAGTGGACACAGCCCCCGATCTCGAGCCCCGGCCCCCGGCTTCTGTCTGGACATTGCATCGCCCTAGTTTTCTTTCTTTA
1681 AAAAAAAAAAATTCCAACAGAAGCCACAGGCCGGAGCCCCTGGGAGCCCTCGCACCGCTCCCCACCCCACTCAGCGGCCC
1761 CATCGGAACTGAGAATTGCAAAACCCCCGACTTTAGCTATAGTTAAGTTGCCTTCCCCCGGTGTCCCCACGTTTGGTGTC
1841 TGGCTATGGCGCCCCCATCTGACTGTCCAGCTCTCTCTCCCCCACCCCTCCCGGTGTTGTTATTAAACGTCTGTGGAGCT
1921 TCTGCTGCAAGGAACAAAAAAGAAAAAAATCAAAAAAGCGACAAAAAAACACAAACAGAAGAGGAAAAAAAAGCAACAAA
2001 AAAAGAAAACACACAGAAGAGATTTAAAAAAAAAAAAGGAAAAAAAAAAAAGACATAAACTGGCACCAGTTAACTTTCTT
2081 GTACTTTTTTGCTGAATTTAGCTTCTTGTAGTTTTAACTTATTGCTATGTTAACTATTTATTCTCCTCGTTGCCCTGTAA
2161 GGACATCCGTGTATATTTCTGTTACTTCATCCGGTTTGCAAGTTAAAGGAACGACAATGTTCTCTTTGTTTCTTTAAGTT
2241 TTGCCGAGACATGGTTATGCCTAATTTATTTATAAAAGGGGAAGTGGAATCATTAAAGTAATAATAATTATTAATAACAG
2321 TAATGGTAGCCGAGTGGCACGCGGGGGCGTGTGCTCTGCGGGACAGTCCCCACGGCCAGCGACGTCCAGGTCACCAATGG
2401 GATTCTTTTTGCTCTTGTCTTGAGAATTTTTTCAGTCCTATTTAGCTGGTGAAATCCCTAGCTTGTTCTTGATACACGAA
2481 CCTATTTATTCTCGTGGTTTTAAGTCCTCCCTGCCCTCTCTCCTCTCCCCTGCAGCAGGGCAGGGACCCCTCTCCCCTGC
2561 TGTCTCTTGGGGCTCTCTCCCCTCCGCCCTCTGCATTCGGGAACACGCACGTCCGCGTGGGAAGCTTGCAGCAGGGCGTC
2641 CGAGCAATAAGGGCTGGGTTTGTCCCCCACCCTGGGGGGGGCCAGGCTCCAGAGAGGGGGCACCTCCCCACACACCCCCC
2721 CCCCACCGAGGCCTAGGCCCCTGCCACCCCCAAGACTGGGAGGGGACTTCTTTTTCTAAAACACAAAACTCAGCCCAGCC
2801 AGGCCCCCTCCCTGGAGGCCAGCCCCTCCCCGCAGGGGGCCAGGCCGGGGCTCCCAGGCGAGGGGGACTTGGGGCTTCCA
2881 CGTCCCTGGGGGGCAGGGGCCAGGCCAGGGGGGAGGGGGCTCAGCCCCTCCACCCCTCCCTTCATCCTGTTATTTATTCG
2961 GAAGGTTTCAAATCACGACAGAGTCCATGTTGGAGGTGATAAAAAACTGTAAAAAAAAAAAAAAAAAAAAAGACAAAACA
3041 AAAGACAAAAAAAAAAAAAAAACCCAACAAGAACACTTCGCGTTGCGTTTAGACTATTTATTACCGGGATCACAGGCCTG
3121 GCCGCGGTAACAGACTTTTACATGGAATTGTTTAATTATTTGTACTTTTCATGCCAGAAAATAAAAGTTCAGAATCTT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ggGGGG--AGGGGCCCUGGg 5'
            ||||  ||||||||:|: 
Target 5' caCCCCTGTCCCCGGGGCTc 3'
 1355 - 1374 138.00 -38.10
2 
miRNA  3' gggggGAGGGGCCCUGgg 5'
               |||: ||||||  
Target 5' gtgtgCTCTGCGGGACag 3'
 2349 - 2366 133.00 -19.00
3 
miRNA  3' ggggggAGGGGCCCUGGg 5'
                |: ||||||:| 
Target 5' tatttaTTACCGGGATCa 3'
 3095 - 3112 132.00 -16.20
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN20079398 26 COSMIC
COSN24340302 28 COSMIC
COSN27959324 40 COSMIC
COSN30516779 45 COSMIC
COSN30509693 53 COSMIC
COSN18742146 55 COSMIC
COSN20054691 57 COSMIC
COSN30538340 59 COSMIC
COSN20294978 490 COSMIC
COSN29940224 495 COSMIC
COSN31572376 520 COSMIC
COSN31563066 559 COSMIC
COSN24306670 568 COSMIC
COSN30542136 568 COSMIC
COSN25896065 575 COSMIC
COSN26464841 649 COSMIC
COSN26557737 667 COSMIC
COSN19661865 669 COSMIC
COSN19656357 677 COSMIC
COSN19661872 679 COSMIC
COSN19655743 680 COSMIC
COSN17907877 729 COSMIC
COSN23091998 735 COSMIC
COSN26600297 759 COSMIC
COSN32057251 808 COSMIC
COSN31571946 975 COSMIC
COSN31603689 1013 COSMIC
COSN8256203 1147 COSMIC
COSN18741568 1163 COSMIC
COSN32057006 1173 COSMIC
COSN26856347 1200 COSMIC
COSN17075787 1229 COSMIC
COSN27415133 1322 COSMIC
COSN27240386 1323 COSMIC
COSN1210743 1503 COSMIC
COSN27205363 1679 COSMIC
COSN27517971 1680 COSMIC
COSN26589868 1710 COSMIC
COSN23426438 1721 COSMIC
COSN7353796 1788 COSMIC
COSN1210741 1804 COSMIC
COSN15661891 2038 COSMIC
COSN20093519 2039 COSMIC
COSN15661890 2040 COSMIC
COSN15290820 2212 COSMIC
COSN1760321 2555 COSMIC
COSN16892373 2937 COSMIC
COSN25987982 2959 COSMIC
COSN9347584 2959 COSMIC
COSN20093517 3032 COSMIC
COSN20093515 3044 COSMIC
COSN20093514 3046 COSMIC
COSN29050568 3046 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs779645608 3 dbSNP
rs757797512 5 dbSNP
rs1484794334 7 dbSNP
rs750136144 9 dbSNP
rs1201093124 10 dbSNP
rs1483574750 11 dbSNP
rs1260006485 13 dbSNP
rs764834247 17 dbSNP
rs1192520096 18 dbSNP
rs1330899498 21 dbSNP
rs1427311219 22 dbSNP
rs1288714676 23 dbSNP
rs761661246 24 dbSNP
rs753613748 25 dbSNP
rs200383181 26 dbSNP
rs760668510 27 dbSNP
rs1179777865 28 dbSNP
rs1187716283 28 dbSNP
rs1471633589 29 dbSNP
rs775255481 29 dbSNP
rs1319078020 31 dbSNP
rs1459180947 32 dbSNP
rs568484090 33 dbSNP
rs1164628546 38 dbSNP
rs1471964787 39 dbSNP
rs1370410416 40 dbSNP
rs1157502851 43 dbSNP
rs1405069186 55 dbSNP
rs1350817219 57 dbSNP
rs35334288 57 dbSNP
rs748089092 57 dbSNP
rs767566028 57 dbSNP
rs911450726 57 dbSNP
rs963554329 59 dbSNP
rs985605661 59 dbSNP
rs1286371994 61 dbSNP
rs1347107089 73 dbSNP
rs1196663104 75 dbSNP
rs936882896 75 dbSNP
rs1024655825 77 dbSNP
rs1180546935 77 dbSNP
rs1440227106 77 dbSNP
rs925424662 77 dbSNP
rs966855065 77 dbSNP
rs977908007 77 dbSNP
rs1469798838 78 dbSNP
rs1273249575 87 dbSNP
rs1016073271 89 dbSNP
rs991785064 91 dbSNP
rs1172540185 93 dbSNP
rs1207583404 105 dbSNP
rs1007789478 110 dbSNP
rs547037629 114 dbSNP
rs1326660016 115 dbSNP
rs1224632060 117 dbSNP
rs1049833660 118 dbSNP
rs1233831144 119 dbSNP
rs369778910 127 dbSNP
rs1342186954 128 dbSNP
rs901135573 130 dbSNP
rs1443709886 131 dbSNP
rs1482553321 134 dbSNP
rs1203419839 138 dbSNP
rs1372421080 139 dbSNP
rs1043041667 148 dbSNP
rs994268769 158 dbSNP
rs1376430586 160 dbSNP
rs1451401279 165 dbSNP
rs1433026582 169 dbSNP
rs1428104883 171 dbSNP
rs1347115613 187 dbSNP
rs200375391 188 dbSNP
rs1414187647 194 dbSNP
rs1436619636 194 dbSNP
rs552353483 198 dbSNP
rs1297744759 199 dbSNP
rs1008154193 205 dbSNP
rs889769517 209 dbSNP
rs1053984953 210 dbSNP
rs1266859894 210 dbSNP
rs1050033568 212 dbSNP
rs112277943 217 dbSNP
rs766524991 224 dbSNP
rs1455497619 226 dbSNP
rs1253581768 227 dbSNP
rs925508709 228 dbSNP
rs926413565 230 dbSNP
rs113632486 234 dbSNP
rs945149656 238 dbSNP
rs1369274994 243 dbSNP
rs1458208620 255 dbSNP
rs991839091 256 dbSNP
rs1458715297 262 dbSNP
rs959058282 267 dbSNP
rs949062470 275 dbSNP
rs1380036403 280 dbSNP
rs1216054811 284 dbSNP
rs1314423063 285 dbSNP
rs984765854 295 dbSNP
rs763575580 297 dbSNP
rs1276914810 298 dbSNP
rs563601251 312 dbSNP
rs972406589 313 dbSNP
rs1349177706 320 dbSNP
rs963347519 322 dbSNP
rs1463546597 324 dbSNP
rs547896436 328 dbSNP
rs986018134 339 dbSNP
rs1297514642 340 dbSNP
rs1423503049 342 dbSNP
rs1415334904 344 dbSNP
rs993711127 345 dbSNP
rs3745686 348 dbSNP
rs1364156032 349 dbSNP
rs1028416123 358 dbSNP
rs774874323 362 dbSNP
rs1289561919 365 dbSNP
rs1395048103 366 dbSNP
rs1291079312 367 dbSNP
rs1352651704 368 dbSNP
rs1229399804 370 dbSNP
rs1288264696 371 dbSNP
rs1315712954 373 dbSNP
rs1447546534 373 dbSNP
rs1008247709 374 dbSNP
rs1310809111 378 dbSNP
rs371309201 380 dbSNP
rs1482981215 387 dbSNP
rs889823172 389 dbSNP
rs998237433 390 dbSNP
rs901187953 394 dbSNP
rs1182701147 398 dbSNP
rs1395553536 416 dbSNP
rs1167874716 429 dbSNP
rs1447649923 431 dbSNP
rs1178175944 438 dbSNP
rs1001132968 464 dbSNP
rs1465119407 465 dbSNP
rs1372093593 466 dbSNP
rs904065028 467 dbSNP
rs1420985324 471 dbSNP
rs1186644535 482 dbSNP
rs1221391183 483 dbSNP
rs1482695080 490 dbSNP
rs1042580610 495 dbSNP
rs1277026582 496 dbSNP
rs1213382018 503 dbSNP
rs945500199 505 dbSNP
rs1205577535 506 dbSNP
rs1486210812 514 dbSNP
rs918078289 520 dbSNP
rs1192793761 521 dbSNP
rs1479550501 521 dbSNP
rs1056251278 523 dbSNP
rs1432088285 533 dbSNP
rs1226799483 535 dbSNP
rs1208358254 540 dbSNP
rs1309835152 546 dbSNP
rs1296330947 547 dbSNP
rs1227845142 549 dbSNP
rs1377168657 553 dbSNP
rs1295366346 558 dbSNP
rs1415693474 559 dbSNP
rs1375838044 560 dbSNP
rs1307221263 561 dbSNP
rs937743064 564 dbSNP
rs1367584042 565 dbSNP
rs559068480 568 dbSNP
rs1299970167 569 dbSNP
rs926314423 569 dbSNP
rs984822366 569 dbSNP
rs1356226604 570 dbSNP
rs952014123 571 dbSNP
rs1247203460 575 dbSNP
rs1305486399 575 dbSNP
rs1468009647 575 dbSNP
rs1170482816 584 dbSNP
rs1422730634 588 dbSNP
rs1249291219 594 dbSNP
rs1415134326 596 dbSNP
rs1185505978 603 dbSNP
rs1447078475 604 dbSNP
rs1417031427 614 dbSNP
rs919138610 622 dbSNP
rs1349783530 623 dbSNP
rs1189606826 638 dbSNP
rs971992519 640 dbSNP
rs1323167746 652 dbSNP
rs1475369390 655 dbSNP
rs1251518280 656 dbSNP
rs541146076 656 dbSNP
rs577009280 659 dbSNP
rs1268634163 662 dbSNP
rs1381694169 665 dbSNP
rs1211677298 667 dbSNP
rs1290277573 667 dbSNP
rs777820255 667 dbSNP
rs1203481604 669 dbSNP
rs1018731062 671 dbSNP
rs1239977834 677 dbSNP
rs879060188 677 dbSNP
rs1286527426 678 dbSNP
rs753451769 679 dbSNP
rs565115544 680 dbSNP
rs1417534317 687 dbSNP
rs1474440256 688 dbSNP
rs1161920804 689 dbSNP
rs1054792230 693 dbSNP
rs1360999445 693 dbSNP
rs1390108729 693 dbSNP
rs56214729 693 dbSNP
rs574010005 693 dbSNP
rs1448968538 695 dbSNP
rs1283833119 696 dbSNP
rs1354508669 696 dbSNP
rs1252413065 698 dbSNP
rs1220858625 710 dbSNP
rs1329825633 713 dbSNP
rs1319990322 722 dbSNP
rs1275204570 723 dbSNP
rs1234092718 724 dbSNP
rs1443668139 727 dbSNP
rs937697773 729 dbSNP
rs1287430310 730 dbSNP
rs1184862241 731 dbSNP
rs1413317718 734 dbSNP
rs1470110877 735 dbSNP
rs866016724 735 dbSNP
rs368875153 736 dbSNP
rs776767853 736 dbSNP
rs954094585 740 dbSNP
rs1300709864 741 dbSNP
rs1028327245 742 dbSNP
rs1238186080 743 dbSNP
rs1001251695 744 dbSNP
rs1362820846 744 dbSNP
rs1219375532 759 dbSNP
rs1418356528 763 dbSNP
rs1020930799 764 dbSNP
rs1345358366 765 dbSNP
rs1009966050 777 dbSNP
rs1009806176 779 dbSNP
rs1166532560 781 dbSNP
rs1437624798 784 dbSNP
rs894091551 785 dbSNP
rs1249033443 786 dbSNP
rs543601133 802 dbSNP
rs938182761 804 dbSNP
rs1180026864 805 dbSNP
rs1421266241 810 dbSNP
rs1393465133 817 dbSNP
rs1251768028 818 dbSNP
rs937744618 819 dbSNP
rs368091614 822 dbSNP
rs1467560345 825 dbSNP
rs1275097950 828 dbSNP
rs554876669 829 dbSNP
rs1048733175 830 dbSNP
rs1440979117 831 dbSNP
rs1302110936 836 dbSNP
rs770742564 837 dbSNP
rs3826813 842 dbSNP
rs1274914600 843 dbSNP
rs1339131390 851 dbSNP
rs918981056 852 dbSNP
rs1452732728 854 dbSNP
rs971787961 857 dbSNP
rs918911222 868 dbSNP
rs748309106 877 dbSNP
rs1368727555 882 dbSNP
rs944618176 884 dbSNP
rs909172567 894 dbSNP
rs985887966 894 dbSNP
rs1327284548 895 dbSNP
rs1392753779 896 dbSNP
rs954174446 898 dbSNP
rs1325153855 900 dbSNP
rs1368064034 907 dbSNP
rs1387384282 908 dbSNP
rs574811401 912 dbSNP
rs553070766 914 dbSNP
rs1325536826 916 dbSNP
rs1225659045 923 dbSNP
rs1286275719 928 dbSNP
rs1357519255 931 dbSNP
rs564177541 933 dbSNP
rs1213556614 936 dbSNP
rs1030142878 939 dbSNP
rs968477850 940 dbSNP
rs1209508176 945 dbSNP
rs570605239 948 dbSNP
rs1427004206 952 dbSNP
rs1189916324 966 dbSNP
rs1453070430 967 dbSNP
rs965477965 971 dbSNP
rs1020953747 974 dbSNP
rs1464513396 975 dbSNP
rs1458337304 979 dbSNP
rs1288981744 991 dbSNP
rs1021449940 995 dbSNP
rs1380116055 997 dbSNP
rs1009610273 1003 dbSNP
rs1356429605 1005 dbSNP
rs1486339420 1013 dbSNP
rs778882292 1014 dbSNP
rs1261043196 1019 dbSNP
rs1032602094 1027 dbSNP
rs1035203120 1030 dbSNP
rs1239825421 1034 dbSNP
rs1260178511 1035 dbSNP
rs768743067 1042 dbSNP
rs1202326755 1047 dbSNP
rs117845984 1051 dbSNP
rs1046570342 1052 dbSNP
rs1187017203 1075 dbSNP
rs537204366 1080 dbSNP
rs905346322 1081 dbSNP
rs1473507620 1087 dbSNP
rs1166932443 1092 dbSNP
rs949539544 1095 dbSNP
rs1049307106 1099 dbSNP
rs897609209 1101 dbSNP
rs1451005027 1106 dbSNP
rs1363819728 1108 dbSNP
rs1036521051 1115 dbSNP
rs897518084 1126 dbSNP
rs1311487417 1128 dbSNP
rs1352750392 1129 dbSNP
rs167516 1136 dbSNP
rs944628207 1138 dbSNP
rs908838869 1140 dbSNP
rs1235586011 1141 dbSNP
rs141996336 1147 dbSNP
rs548895949 1148 dbSNP
rs931864653 1153 dbSNP
rs1209032349 1157 dbSNP
rs1255074790 1159 dbSNP
rs1433256399 1163 dbSNP
rs923165405 1164 dbSNP
rs1174803990 1172 dbSNP
rs975943060 1173 dbSNP
rs1416782303 1177 dbSNP
rs967613760 1178 dbSNP
rs1395279789 1182 dbSNP
rs530304402 1185 dbSNP
rs1171386992 1202 dbSNP
rs559343867 1204 dbSNP
rs1195479824 1219 dbSNP
rs979965031 1220 dbSNP
rs1371867009 1228 dbSNP
rs988595686 1231 dbSNP
rs958076174 1232 dbSNP
rs547067918 1237 dbSNP
rs186459680 1238 dbSNP
rs1277038542 1241 dbSNP
rs182278764 1243 dbSNP
rs750786346 1244 dbSNP
rs1025175965 1248 dbSNP
rs1479642323 1250 dbSNP
rs1013749378 1255 dbSNP
rs781744976 1256 dbSNP
rs139926964 1260 dbSNP
rs145991094 1261 dbSNP
rs757685552 1280 dbSNP
rs142535942 1281 dbSNP
rs1170237919 1289 dbSNP
rs931624197 1301 dbSNP
rs1008489493 1302 dbSNP
rs1327174661 1304 dbSNP
rs923222657 1307 dbSNP
rs1438882603 1318 dbSNP
rs1040275631 1324 dbSNP
rs1362980572 1324 dbSNP
rs1429648783 1327 dbSNP
rs542936485 1328 dbSNP
rs913027058 1329 dbSNP
rs114348792 1330 dbSNP
rs958385282 1331 dbSNP
rs981464148 1332 dbSNP
rs1028240693 1333 dbSNP
rs763546092 1333 dbSNP
rs1234981069 1334 dbSNP
rs1450972086 1334 dbSNP
rs754879241 1334 dbSNP
rs1285687589 1336 dbSNP
rs1334116782 1336 dbSNP
rs543455540 1336 dbSNP
rs544515244 1338 dbSNP
rs969360235 1340 dbSNP
rs1189160654 1341 dbSNP
rs1417740983 1344 dbSNP
rs1474492484 1345 dbSNP
rs961783859 1355 dbSNP
rs1408737018 1361 dbSNP
rs1400199183 1362 dbSNP
rs1025186018 1367 dbSNP
rs1361166799 1368 dbSNP
rs1416358637 1370 dbSNP
rs1313219212 1374 dbSNP
rs1013759149 1380 dbSNP
rs1354605254 1382 dbSNP
rs553375976 1390 dbSNP
rs1015484355 1391 dbSNP
rs1339461945 1399 dbSNP
rs757915974 1406 dbSNP
rs752413167 1414 dbSNP
rs890160115 1422 dbSNP
rs1050043884 1424 dbSNP
rs1006372070 1433 dbSNP
rs534660764 1438 dbSNP
rs1484903431 1440 dbSNP
rs764970946 1441 dbSNP
rs1175595858 1446 dbSNP
rs1043292170 1454 dbSNP
rs865879081 1460 dbSNP
rs1173262532 1461 dbSNP
rs192366977 1461 dbSNP
rs558590483 1472 dbSNP
rs1412606942 1474 dbSNP
rs868608846 1475 dbSNP
rs1040327425 1482 dbSNP
rs1277228413 1483 dbSNP
rs945930244 1484 dbSNP
rs1411081189 1485 dbSNP
rs1279168011 1492 dbSNP
rs891691898 1499 dbSNP
rs1274284449 1500 dbSNP
rs1437715782 1504 dbSNP
rs980757475 1513 dbSNP
rs948323081 1519 dbSNP
rs537130912 1528 dbSNP
rs1193235320 1529 dbSNP
rs1054723278 1540 dbSNP
rs771524803 1541 dbSNP
rs759470826 1546 dbSNP
rs1409255565 1554 dbSNP
rs1329249476 1565 dbSNP
rs569940739 1570 dbSNP
rs925412832 1576 dbSNP
rs980786360 1578 dbSNP
rs948065732 1579 dbSNP
rs917890117 1580 dbSNP
rs1194516596 1587 dbSNP
rs1183901625 1591 dbSNP
rs1468952545 1593 dbSNP
rs1438368060 1595 dbSNP
rs992442188 1600 dbSNP
rs1448920338 1601 dbSNP
rs1251810025 1602 dbSNP
rs1211305210 1603 dbSNP
rs1474965061 1604 dbSNP
rs962632923 1608 dbSNP
rs1015164965 1617 dbSNP
rs985399118 1618 dbSNP
rs952150907 1623 dbSNP
rs776544004 1624 dbSNP
rs778759174 1630 dbSNP
rs904172492 1631 dbSNP
rs1318571417 1634 dbSNP
rs1443529936 1637 dbSNP
rs1370933113 1638 dbSNP
rs1323921485 1643 dbSNP
rs554859375 1645 dbSNP
rs1443209488 1654 dbSNP
rs536992960 1657 dbSNP
rs1010153244 1661 dbSNP
rs371526572 1662 dbSNP
rs1056951048 1673 dbSNP
rs1205875916 1675 dbSNP
rs1399246142 1677 dbSNP
rs1261380006 1679 dbSNP
rs757125510 1679 dbSNP
rs906591924 1680 dbSNP
rs78076958 1691 dbSNP
rs779987842 1692 dbSNP
rs948108690 1692 dbSNP
rs1470444464 1696 dbSNP
rs1366135006 1702 dbSNP
rs1454954549 1706 dbSNP
rs188926056 1708 dbSNP
rs1158769747 1712 dbSNP
rs1394437428 1713 dbSNP
rs1398506485 1730 dbSNP
rs532116460 1731 dbSNP
rs1379535457 1732 dbSNP
rs138155859 1733 dbSNP
rs374445012 1735 dbSNP
rs1253157910 1736 dbSNP
rs549937322 1737 dbSNP
rs1444969581 1739 dbSNP
rs1009889458 1748 dbSNP
rs1213316777 1751 dbSNP
rs1282120079 1751 dbSNP
rs891740187 1752 dbSNP
rs1347704574 1755 dbSNP
rs1054373935 1756 dbSNP
rs908060232 1759 dbSNP
rs1237223446 1762 dbSNP
rs1362739310 1763 dbSNP
rs1417430294 1764 dbSNP
rs531412891 1765 dbSNP
rs1288406877 1773 dbSNP
rs1434566069 1778 dbSNP
rs1301593352 1789 dbSNP
rs1376229110 1789 dbSNP
rs905672388 1794 dbSNP
rs1306335539 1804 dbSNP
rs1428866392 1808 dbSNP
rs1374781437 1811 dbSNP
rs1236686805 1812 dbSNP
rs1340778086 1819 dbSNP
rs35525624 1820 dbSNP
rs987563552 1820 dbSNP
rs1254137030 1824 dbSNP
rs1045141785 1829 dbSNP
rs1347203990 1830 dbSNP
rs1196329598 1831 dbSNP
rs773132843 1835 dbSNP
rs777396685 1840 dbSNP
rs1319997362 1844 dbSNP
rs1437284962 1852 dbSNP
rs370159925 1853 dbSNP
rs1420094924 1856 dbSNP
rs1395371121 1871 dbSNP
rs1374685732 1873 dbSNP
rs1299114777 1874 dbSNP
rs1174448266 1877 dbSNP
rs917983228 1878 dbSNP
rs992597142 1883 dbSNP
rs1455562652 1885 dbSNP
rs1298107724 1886 dbSNP
rs1028768636 1893 dbSNP
rs940695185 1894 dbSNP
rs1304413205 1895 dbSNP
rs1370582098 1902 dbSNP
rs1395136385 1907 dbSNP
rs768628925 1908 dbSNP
rs974886848 1909 dbSNP
rs1474197459 1935 dbSNP
rs1243490261 1942 dbSNP
rs1257417656 1943 dbSNP
rs1216884495 1949 dbSNP
rs1215080233 1950 dbSNP
rs1253209545 1950 dbSNP
rs1485544302 1950 dbSNP
rs1487933474 1951 dbSNP
rs1189122687 1957 dbSNP
rs1422009529 1959 dbSNP
rs1429548076 1962 dbSNP
rs1278723790 1969 dbSNP
rs1213610476 1970 dbSNP
rs1422163712 1970 dbSNP
rs1463051024 1970 dbSNP
rs1349298509 1972 dbSNP
rs1294887740 1978 dbSNP
rs1393860013 1980 dbSNP
rs985025693 1980 dbSNP
rs1212914682 1981 dbSNP
rs1364267741 1983 dbSNP
rs1270974020 1984 dbSNP
rs1277462651 1985 dbSNP
rs1316036884 1985 dbSNP
rs968449454 1985 dbSNP
rs1235097322 1990 dbSNP
rs1273219970 1993 dbSNP
rs1320609088 1993 dbSNP
rs755257868 1993 dbSNP
rs1243543520 1994 dbSNP
rs1374591249 1995 dbSNP
rs1310895296 1997 dbSNP
rs1010161272 1998 dbSNP
rs1261519854 1999 dbSNP
rs962864116 2005 dbSNP
rs1166300454 2008 dbSNP
rs1359700735 2010 dbSNP
rs1169047122 2012 dbSNP
rs1407725110 2014 dbSNP
rs1413081415 2015 dbSNP
rs1421099143 2019 dbSNP
rs1186743221 2021 dbSNP
rs1474700521 2025 dbSNP
rs561184608 2026 dbSNP
rs1431162830 2032 dbSNP
rs1297317418 2033 dbSNP
rs1338177069 2037 dbSNP
rs1226163032 2038 dbSNP
rs1293902801 2038 dbSNP
rs547645144 2038 dbSNP
rs1255823798 2039 dbSNP
rs1215472256 2040 dbSNP
rs1317098494 2040 dbSNP
rs1002684001 2041 dbSNP
rs1284248804 2041 dbSNP
rs1189266917 2049 dbSNP
rs75426143 2051 dbSNP
rs1237471139 2052 dbSNP
rs1419281254 2052 dbSNP
rs532683569 2052 dbSNP
rs78477738 2052 dbSNP
rs905674333 2053 dbSNP
rs1358320088 2060 dbSNP
rs1251248840 2066 dbSNP
rs1436417151 2070 dbSNP
rs1313384199 2074 dbSNP
rs1045180686 2075 dbSNP
rs1203104990 2091 dbSNP
rs1309329941 2092 dbSNP
rs1273851056 2094 dbSNP
rs1232875485 2095 dbSNP
rs1353819039 2096 dbSNP
rs1291163903 2100 dbSNP
rs1416391060 2101 dbSNP
rs1445773955 2110 dbSNP
rs948126353 2116 dbSNP
rs952202381 2122 dbSNP
rs1238887988 2127 dbSNP
rs1306714972 2128 dbSNP
rs1297528509 2130 dbSNP
rs749352649 2136 dbSNP
rs1350489164 2138 dbSNP
rs899202548 2141 dbSNP
rs1278499209 2142 dbSNP
rs1029142501 2148 dbSNP
rs1441155424 2149 dbSNP
rs1440022047 2155 dbSNP
rs1355745125 2167 dbSNP
rs143597166 2168 dbSNP
rs974889143 2169 dbSNP
rs1233473203 2171 dbSNP
rs1439172019 2173 dbSNP
rs1460549865 2189 dbSNP
rs965811550 2192 dbSNP
rs940953083 2193 dbSNP
rs183587442 2194 dbSNP
rs1401261091 2197 dbSNP
rs1167468324 2210 dbSNP
rs560142919 2212 dbSNP
rs1010355467 2215 dbSNP
rs955692587 2216 dbSNP
rs191775580 2217 dbSNP
rs1400383250 2218 dbSNP
rs1445478535 2224 dbSNP
rs1328845512 2226 dbSNP
rs1051949973 2228 dbSNP
rs1000091095 2229 dbSNP
rs933522048 2234 dbSNP
rs576859480 2235 dbSNP
rs751780406 2236 dbSNP
rs1343334838 2239 dbSNP
rs905725343 2245 dbSNP
rs1274408313 2251 dbSNP
rs968834890 2256 dbSNP
rs1196870744 2267 dbSNP
rs186495240 2269 dbSNP
rs766572253 2283 dbSNP
rs555892783 2284 dbSNP
rs1233478245 2289 dbSNP
rs1376788303 2296 dbSNP
rs1213174983 2302 dbSNP
rs1014030325 2304 dbSNP
rs988447801 2306 dbSNP
rs955959635 2309 dbSNP
rs1332862156 2312 dbSNP
rs1035631008 2314 dbSNP
rs896610302 2318 dbSNP
rs1234190818 2321 dbSNP
rs1056551188 2325 dbSNP
rs1322101931 2332 dbSNP
rs1329709922 2334 dbSNP
rs1433386214 2336 dbSNP
rs1306079998 2340 dbSNP
rs940694021 2341 dbSNP
rs1217356788 2342 dbSNP
rs1275067123 2343 dbSNP
rs543554643 2343 dbSNP
rs1049796056 2344 dbSNP
rs76880598 2345 dbSNP
rs775751854 2348 dbSNP
rs974939774 2349 dbSNP
rs1195114935 2351 dbSNP
rs758494814 2352 dbSNP
rs1391758054 2358 dbSNP
rs1263671076 2359 dbSNP
rs769976972 2360 dbSNP
rs1458130934 2373 dbSNP
rs911631769 2374 dbSNP
rs1458030680 2377 dbSNP
rs1012334560 2380 dbSNP
rs1157401611 2381 dbSNP
rs1421354498 2384 dbSNP
rs988546776 2385 dbSNP
rs1402113962 2390 dbSNP
rs1367945450 2397 dbSNP
rs1381475455 2405 dbSNP
rs1193019801 2408 dbSNP
rs150329191 2417 dbSNP
rs768262774 2422 dbSNP
rs746283996 2423 dbSNP
rs747422325 2423 dbSNP
rs955912470 2427 dbSNP
rs1214525434 2428 dbSNP
rs1295723035 2435 dbSNP
rs1341125625 2446 dbSNP
rs1244220770 2456 dbSNP
rs1263873712 2467 dbSNP
rs1353066473 2468 dbSNP
rs1032658459 2476 dbSNP
rs1004813429 2477 dbSNP
rs999802453 2478 dbSNP
rs1191416141 2483 dbSNP
rs1259606157 2485 dbSNP
rs969977520 2491 dbSNP
rs1423855398 2492 dbSNP
rs781435684 2493 dbSNP
rs149256658 2494 dbSNP
rs1052002561 2510 dbSNP
rs757604112 2511 dbSNP
rs1418316255 2512 dbSNP
rs1403594433 2517 dbSNP
rs536397658 2517 dbSNP
rs1309903387 2521 dbSNP
rs566383889 2523 dbSNP
rs1368110977 2529 dbSNP
rs1305053868 2530 dbSNP
rs1241066119 2532 dbSNP
rs1308893284 2535 dbSNP
rs530582137 2541 dbSNP
rs554136591 2542 dbSNP
rs1352701928 2546 dbSNP
rs1214245689 2551 dbSNP
rs1257571324 2553 dbSNP
rs539323398 2555 dbSNP
rs1483507084 2557 dbSNP
rs1386942814 2561 dbSNP
rs961334290 2561 dbSNP
rs947171121 2564 dbSNP
rs1178847964 2571 dbSNP
rs1383829605 2572 dbSNP
rs571292893 2577 dbSNP
rs1438188352 2578 dbSNP
rs1004944826 2581 dbSNP
rs1175878331 2583 dbSNP
rs1378597263 2585 dbSNP
rs914282816 2586 dbSNP
rs549800327 2587 dbSNP
rs1173553118 2588 dbSNP
rs531476487 2599 dbSNP
rs988541264 2601 dbSNP
rs1330911397 2605 dbSNP
rs567211543 2606 dbSNP
rs930621416 2607 dbSNP
rs549167868 2608 dbSNP
rs1396947403 2610 dbSNP
rs922217145 2611 dbSNP
rs1312558355 2612 dbSNP
rs1232434620 2613 dbSNP
rs1428104318 2613 dbSNP
rs1039262185 2615 dbSNP
rs1203100930 2616 dbSNP
rs1198770181 2617 dbSNP
rs1489141849 2631 dbSNP
rs944862436 2633 dbSNP
rs1237066733 2636 dbSNP
rs535690633 2637 dbSNP
rs988987770 2638 dbSNP
rs981395763 2641 dbSNP
rs955841875 2642 dbSNP
rs1174565120 2654 dbSNP
rs35932742 2659 dbSNP
rs1375340814 2662 dbSNP
rs925681450 2664 dbSNP
rs561905305 2666 dbSNP
rs1398213219 2667 dbSNP
rs1286522990 2668 dbSNP
rs1294404245 2669 dbSNP
rs1368023113 2670 dbSNP
rs969694396 2671 dbSNP
rs1299065889 2673 dbSNP
rs1022840365 2675 dbSNP
rs1256808564 2676 dbSNP
rs1315538245 2679 dbSNP
rs995717027 2680 dbSNP
rs1016333621 2682 dbSNP
rs963510068 2682 dbSNP
rs560277500 2685 dbSNP
rs992674649 2691 dbSNP
rs34915442 2701 dbSNP
rs985504633 2702 dbSNP
rs886483034 2704 dbSNP
rs1325426042 2705 dbSNP
rs1421565580 2710 dbSNP
rs1292978321 2712 dbSNP
rs954091441 2714 dbSNP
rs1341491118 2715 dbSNP
rs1291525231 2716 dbSNP
rs1349556878 2716 dbSNP
rs544926982 2716 dbSNP
rs542073024 2717 dbSNP
rs997787118 2717 dbSNP
rs1039222634 2718 dbSNP
rs1377835101 2719 dbSNP
rs1004440541 2720 dbSNP
rs1261713480 2721 dbSNP
rs947565642 2721 dbSNP
rs886557117 2722 dbSNP
rs893296052 2722 dbSNP
rs1362216125 2723 dbSNP
rs564976823 2723 dbSNP
rs934395124 2724 dbSNP
rs1311070091 2725 dbSNP
rs140160574 2725 dbSNP
rs571280193 2725 dbSNP
rs928286808 2726 dbSNP
rs1027735326 2727 dbSNP
rs1211455391 2728 dbSNP
rs1284364824 2728 dbSNP
rs543370794 2728 dbSNP
rs998183032 2734 dbSNP
rs1371468129 2740 dbSNP
rs1482357618 2741 dbSNP
rs1195595130 2743 dbSNP
rs1180732675 2752 dbSNP
rs1381207999 2752 dbSNP
rs994839922 2753 dbSNP
rs900468322 2762 dbSNP
rs1478298466 2763 dbSNP
rs1263568135 2764 dbSNP
rs1186996450 2766 dbSNP
rs1157790677 2772 dbSNP
rs1405553198 2774 dbSNP
rs1416032558 2775 dbSNP
rs1334826083 2778 dbSNP
rs1484611131 2783 dbSNP
rs1358056010 2786 dbSNP
rs1445232492 2788 dbSNP
rs981069496 2796 dbSNP
rs948676051 2798 dbSNP
rs973996355 2804 dbSNP
rs962899409 2817 dbSNP
rs1201019755 2823 dbSNP
rs1015380641 2831 dbSNP
rs576483144 2832 dbSNP
rs983504717 2835 dbSNP
rs1238904776 2841 dbSNP
rs1457448346 2843 dbSNP
rs1177003946 2844 dbSNP
rs1338173617 2847 dbSNP
rs1478918585 2856 dbSNP
rs1249152144 2857 dbSNP
rs950770452 2858 dbSNP
rs1030303056 2859 dbSNP
rs1318184488 2860 dbSNP
rs1293024109 2862 dbSNP
rs182533486 2866 dbSNP
rs1333179943 2871 dbSNP
rs944954532 2875 dbSNP
rs1274747514 2879 dbSNP
rs138354612 2882 dbSNP
rs1391391036 2885 dbSNP
rs1258733786 2889 dbSNP
rs893346976 2892 dbSNP
rs1196965828 2894 dbSNP
rs1292347312 2896 dbSNP
rs554385350 2897 dbSNP
rs1453082230 2898 dbSNP
rs1199252997 2901 dbSNP
rs1394394273 2901 dbSNP
rs1053321517 2902 dbSNP
rs1245932881 2906 dbSNP
rs1195758532 2908 dbSNP
rs1479381858 2908 dbSNP
rs539389362 2909 dbSNP
rs934403693 2912 dbSNP
rs578247953 2913 dbSNP
rs1165772899 2914 dbSNP
rs1466302358 2914 dbSNP
rs1417420052 2916 dbSNP
rs906921133 2917 dbSNP
rs1344045259 2921 dbSNP
rs574176645 2922 dbSNP
rs1402389523 2925 dbSNP
rs1163864872 2927 dbSNP
rs1045417648 2931 dbSNP
rs948411051 2936 dbSNP
rs915574311 2937 dbSNP
rs974438451 2938 dbSNP
rs1474093234 2941 dbSNP
rs1239052698 2947 dbSNP
rs373766267 2959 dbSNP
rs1437219069 2960 dbSNP
rs1288799999 2963 dbSNP
rs1489817974 2964 dbSNP
rs1218689295 2966 dbSNP
rs1247748532 2975 dbSNP
rs1477193058 2976 dbSNP
rs1369722978 2977 dbSNP
rs1263120765 2981 dbSNP
rs1472890995 2982 dbSNP
rs908393059 2984 dbSNP
rs1364084088 2985 dbSNP
rs1200021529 2986 dbSNP
rs1318096290 2987 dbSNP
rs370492835 2991 dbSNP
rs1360401056 2992 dbSNP
rs567379211 2993 dbSNP
rs1358905352 2995 dbSNP
rs1285199032 2998 dbSNP
rs1238896962 3000 dbSNP
rs1244327396 3006 dbSNP
rs1313264854 3007 dbSNP
rs1242106381 3010 dbSNP
rs1257582387 3011 dbSNP
rs1277167236 3011 dbSNP
rs1286518294 3011 dbSNP
rs1442548007 3011 dbSNP
rs1487895488 3011 dbSNP
rs1186369215 3016 dbSNP
rs1383040841 3019 dbSNP
rs1334542154 3025 dbSNP
rs1330901453 3027 dbSNP
rs1447627251 3031 dbSNP
rs1020824895 3032 dbSNP
rs1159575337 3032 dbSNP
rs1305180273 3032 dbSNP
rs1334934171 3032 dbSNP
rs1379348360 3032 dbSNP
rs1380088752 3032 dbSNP
rs1395574688 3032 dbSNP
rs1452180074 3032 dbSNP
rs950823031 3032 dbSNP
rs982551533 3032 dbSNP
rs1030397789 3033 dbSNP
rs77049617 3034 dbSNP
rs1324124360 3035 dbSNP
rs1463246461 3036 dbSNP
rs1458773508 3039 dbSNP
rs373036949 3039 dbSNP
rs976179986 3039 dbSNP
rs1415664113 3040 dbSNP
rs1163650298 3042 dbSNP
rs1178842977 3044 dbSNP
rs1417765085 3044 dbSNP
rs878940568 3044 dbSNP
rs1466008012 3045 dbSNP
rs879016342 3046 dbSNP
rs1402568402 3047 dbSNP
rs369400019 3047 dbSNP
rs375969105 3047 dbSNP
rs965107216 3047 dbSNP
rs1017601175 3048 dbSNP
rs1389497689 3049 dbSNP
rs1303171438 3055 dbSNP
rs1362002937 3055 dbSNP
rs1179911009 3057 dbSNP
rs1457813029 3058 dbSNP
rs1012244112 3059 dbSNP
rs1260629689 3060 dbSNP
rs1251909561 3061 dbSNP
rs770710524 3062 dbSNP
rs1170215691 3063 dbSNP
rs1193246418 3063 dbSNP
rs1268523756 3063 dbSNP
rs1295004946 3063 dbSNP
rs1338863073 3063 dbSNP
rs1352935791 3063 dbSNP
rs1425851802 3063 dbSNP
rs1433043414 3063 dbSNP
rs1478225433 3063 dbSNP
rs1479845275 3063 dbSNP
rs200985797 3065 dbSNP
rs1452993743 3067 dbSNP
rs1404245407 3068 dbSNP
rs1290984231 3071 dbSNP
rs1031855535 3074 dbSNP
rs1218763003 3074 dbSNP
rs1294259028 3079 dbSNP
rs998998272 3080 dbSNP
rs553286536 3081 dbSNP
rs1490304441 3082 dbSNP
rs1220896708 3085 dbSNP
rs1266041055 3086 dbSNP
rs907349743 3087 dbSNP
rs934817147 3093 dbSNP
rs1241582910 3095 dbSNP
rs775190437 3103 dbSNP
rs925765247 3106 dbSNP
rs1438820101 3111 dbSNP
rs1350718616 3124 dbSNP
rs978443086 3125 dbSNP
rs1399912402 3126 dbSNP
rs1012668028 3133 dbSNP
rs1362327277 3133 dbSNP
rs1400569190 3134 dbSNP
rs745322274 3159 dbSNP
rs1359269915 3162 dbSNP
rs1229659705 3165 dbSNP
rs948398852 3183 dbSNP
rs1359126905 3188 dbSNP
rs1160379123 3194 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084065. RNA binding protein: AGO2. Condition:CLIP_emetine_AbnovaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' ggggggaggggCCCUGgg 5'
                     |||:|  
Target 5' gagagagagaaGGGGC-- 3'
 		32 - 47
Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084065
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_emetine_AbnovaAb
Location of target site ENST00000359227.3 | 3UTR | CAGCAGAGAGAGAGAGAGAGAAAGAGAGAGAGAGAGAGAGAAGGGGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
29 hsa-miR-4281 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT218864 CDKN1A cyclin dependent kinase inhibitor 1A 2 4
MIRT248841 SESN2 sestrin 2 2 2
MIRT452803 PACS2 phosphofurin acidic cluster sorting protein 2 2 2
MIRT462825 BCL3 B-cell CLL/lymphoma 3 2 2
MIRT467812 SLC29A2 solute carrier family 29 member 2 2 2
MIRT477707 EFHD2 EF-hand domain family member D2 2 2
MIRT490047 PRRT2 proline rich transmembrane protein 2 2 2
MIRT491813 ZBTB7B zinc finger and BTB domain containing 7B 2 2
MIRT528905 THBS2 thrombospondin 2 2 2
MIRT534124 SNX33 sorting nexin 33 2 2
MIRT550881 IBA57 IBA57 homolog, iron-sulfur cluster assembly 2 2
MIRT551263 PSMG1 proteasome assembly chaperone 1 2 2
MIRT551984 UGT2B10 UDP glucuronosyltransferase family 2 member B10 2 2
MIRT608916 NCDN neurochondrin 2 6
MIRT637226 TMEM59 transmembrane protein 59 2 2
MIRT644445 ALDOC aldolase, fructose-bisphosphate C 2 2
MIRT657162 IP6K1 inositol hexakisphosphate kinase 1 2 2
MIRT658754 ELAVL3 ELAV like RNA binding protein 3 2 2
MIRT668304 FOSL2 FOS like 2, AP-1 transcription factor subunit 2 2
MIRT687548 MLEC malectin 2 2
MIRT690860 PLEKHG2 pleckstrin homology and RhoGEF domain containing G2 2 2
MIRT697063 PSMC4 proteasome 26S subunit, ATPase 4 2 2
MIRT700860 PER2 period circadian clock 2 2 2
MIRT705073 C4orf29 abhydrolase domain containing 18 2 2
MIRT713930 PIGR polymeric immunoglobulin receptor 2 2
MIRT715904 MFRP membrane frizzled-related protein 2 2
MIRT716060 SFTPB surfactant protein B 2 2
MIRT716729 APOL6 apolipoprotein L6 2 2
MIRT723382 NFAM1 NFAT activating protein with ITAM motif 1 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-4 Dexamethasone approved 5743 Microarray primary rat thymocytes 20847043 2010 up-regulated
miR-4281 Ginsenoside Rh2 NULL 119307 Microarray NSCLC cell line A549 23152132 2013 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-4281 Cisplatin 5460033 NSC119875 approved resistant High Non-Small Cell Lung Cancer cell line (A549)
hsa-miR-4281 Platinum 23939 sensitive High Ovarian Cancer tissue
hsa-miR-4281 Vemurafenib 42611257 NSC761431 approved sensitive High Melanoma cell line (PLX4032, LM16)
hsa-miR-4281 Cisplatin 5460033 NSC119875 approved resistant High Pancreatic Cancer cell line (BxPC-3)
hsa-miR-4281 Vemurafenib 42611257 NSC761431 approved resistant High Melanoma cell line (A375)
hsa-miR-4281 Paclitaxel 36314 NSC125973 approved resistant High Non-Small Cell Lung Cancer cell line (A549)
hsa-miR-4281 Paclitaxel 36314 NSC125973 approved resistant High Non-Small Cell Lung Cancer cell line (H460)
hsa-mir-4281 Dabrafenib 44462760 NSC764134 approved resistant cell line (A375)
hsa-mir-4281 Cisplatin 5460033 NSC119875 approved sensitive cell line
hsa-mir-4281 Doxorubicin 31703 NSC123127 approved resistant cell line (BAS)
hsa-mir-4281 Doxorubicin 31703 NSC123127 approved resistant cell line (HS578T)
hsa-mir-4281 Paclitaxel 36314 NSC125973 approved resistant cell line (W1)
hsa-mir-4281 Androstenedione+Letrozole sensitive cell line (MCF-7)
hsa-mir-4281 Cisplatin 5460033 NSC119875 approved resistant cell line (BxPC3)
hsa-miR-4281 Temozolomide 5394 NSC362856 approved resistant cell line (U251)
hsa-miR-4281 Tamoxifen+Fulvestrant resistant cell line (LCC9)
hsa-miR-4281 Cisplatin 5460033 NSC119875 approved resistant cell line (CIS)
hsa-miR-4281 Cisplatin 5460033 NSC119875 approved resistant cell line (CP20)
hsa-miR-4281 Osimertinib 71496458 NSC779217 approved sensitive cell line (H1975)
hsa-miR-4281 Cisplatin 5460033 NSC119875 approved resistant cell line (A549)
hsa-miR-4281 Vemurafenib 42611257 NSC761431 approved sensitive cell line (LM16)
hsa-miR-4281 Neoadjuvant chemotherapy sensitive tissue (breast cancer)
hsa-miR-4281 Cisplatin 5460033 NSC119875 approved sensitive cell line (TOV-112D)
hsa-miR-4281 Cisplatin 5460033 NSC119875 approved resistant cell line (OVSAHO)

Error report submission