pre-miRNA Information
pre-miRNA hsa-mir-4446   
Genomic Coordinates chr3: 113594876 - 113594942
Description Homo sapiens miR-4446 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4446-5p
Sequence 8| AUUUCCCUGCCAUUCCCUUGGC |29
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1356015605 6 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol DNAJB5   
Synonyms Hsc40
Description DnaJ heat shock protein family (Hsp40) member B5
Transcript NM_001135004   
Other Transcripts NM_001135005 , NM_012266   
Expression
Putative miRNA Targets on DNAJB5
3'UTR of DNAJB5
(miRNA target sites are highlighted)
>DNAJB5|NM_001135004|3'UTR
   1 GCTCTGCCCCAGCCAGTCCAGAGCCTACCACAGCAATACCCCCAACACTCACTCCACTCAATGTGCACCCAGCTTGATGT
  81 CCACTGGACACTGGCAACTTTTTCTAAAATGCAAAAAAAAGCCACTGGTTTTCAGGAAAATGTTCCTGTCCCTGACCCCT
 161 TTTAGAGCTGGGCTGCCTGGGGGGAGTGGGAGGGAGGTGGGGAGAGCTAGCCCAGGCCAGGGGTCAATGTTCATGTCACT
 241 AGCTTTCAATCCAGTTTCCACTGCAGTGGCTGGAGAGTGACCTGAGTGCTACTTGAAGATATGTAGAGATTCCTTATCCA
 321 TGCCTGTACATAGCATGTCCTCCTCCCCTCAGCTTTGCTAATACCAGTCCCCTCCCTTCCCTTTGGCTTCCTGCTGTTGG
 401 GGGTGGAAAAGACTAGAAAGGACATTGCTTTCTCAGCCCCACCCCCAGGTCCACAGTGTCCAAGGTAATGGCACACATAT
 481 TCATGCACAAGAAGCACTCACCTAGAGCTGTCTGTGCCTCTCTGGGAGAAAGGAGAGAGGATAAGAAGGGAAAGTTCACA
 561 ACCTGTGAACAGGGACTTGAGCACGAGACACTTTTCATCTGGAGCAGGGGGGTGAGCTCCTCAGCAGCCTCCGTAACAGC
 641 TGCCCTGCCTACACCTGCAGAGCTGGAGGTTCTGTCCTCCCTGCTGCTCTCAGGAGTGTTCAAGGGTGGAGGGCAGGGAA
 721 TGGGGCCTGAGGTATTAAGGCTAAGAGGTGGGGGACAGGGCCCATCTACCAGCCTTCATCAGGAAGGGAAAGGGCTTTGG
 801 GGTCAGGTGGCAGCTATTCCCCACCAAGAGATTCAGGGTCACAGGTTTTTCCCCACACCTCTGAACTCAGGGCCTAGCCA
 881 CCCCCAAACTTCCAAATCCCACTTTTGTGATATGTGAAGCTACTCATTTCTTACCCTTGGAGGCTGTGTGGGGAATTTCC
 961 AGCCCTTTTATGCCTGTGTGATCCCACCCCACCCCCATAGTTGTATAAAGGTCATAGTGAAGAAGCTGGGGGAAGACTGC
1041 TTCAGCCAGATCCTGGGGTGGGGTCTTTAGGTTTTCTCACTTTGACAACCCCCGAATGTTTTTATAGTAGTTTTTTTGTA
1121 TTTTTTTGTAATGACAGTATTATGTAAAAAATAAAGTATTTTAAAAATATGGCAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' cgGUUCC--CUUACCGUCCCUUua 5'
            |||||  |:| |||||||||  
Target 5' ttCAAGGGTGGAGGGCAGGGAAtg 3'
699 - 722 158.00 -26.70
2
miRNA  3' cggUUCCCUUA-CCGUCCCUUUa 5'
             :|| |:||  | ||||||| 
Target 5' ggaGAGAGGATAAGAAGGGAAAg 3'
532 - 554 155.00 -13.40
3
miRNA  3' cgguucccuuaCCGUCCCUUUa 5'
                     || ||||||| 
Target 5' agccttcatcaGGAAGGGAAAg 3'
771 - 792 147.00 -13.36
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN26973735 8 COSMIC
COSN30450408 10 COSMIC
COSN26973736 21 COSMIC
COSN1378300 121 COSMIC
COSN30711457 121 COSMIC
COSN30727328 143 COSMIC
COSN20076771 266 COSMIC
COSN6390030 268 COSMIC
COSN14769963 314 COSMIC
COSN14760468 472 COSMIC
COSN31513665 685 COSMIC
COSN28656328 748 COSMIC
COSN31553747 961 COSMIC
COSN31550275 1081 COSMIC
COSN26559928 1121 COSMIC
COSN31516825 1123 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs373116154 2 dbSNP
rs766874046 4 dbSNP
rs752033759 7 dbSNP
rs1226872177 9 dbSNP
rs1274318724 10 dbSNP
rs755598003 15 dbSNP
rs914891315 16 dbSNP
rs763519432 20 dbSNP
rs753416773 23 dbSNP
rs1299690790 25 dbSNP
rs908530318 26 dbSNP
rs756893350 28 dbSNP
rs1261875847 32 dbSNP
rs1451195683 34 dbSNP
rs778591083 36 dbSNP
rs745632299 37 dbSNP
rs368959542 40 dbSNP
rs1430383682 41 dbSNP
rs779994915 43 dbSNP
rs746936238 47 dbSNP
rs1348330966 55 dbSNP
rs534902052 59 dbSNP
rs770051452 62 dbSNP
rs921319578 66 dbSNP
rs1367480334 68 dbSNP
rs1265524389 72 dbSNP
rs910724675 73 dbSNP
rs770118094 74 dbSNP
rs1384257296 76 dbSNP
rs931820652 79 dbSNP
rs780509105 81 dbSNP
rs1288217633 82 dbSNP
rs1325571945 86 dbSNP
rs763366601 90 dbSNP
rs558001237 92 dbSNP
rs759958343 94 dbSNP
rs1263707876 96 dbSNP
rs768131436 98 dbSNP
rs1217751380 103 dbSNP
rs890404627 104 dbSNP
rs1465862475 106 dbSNP
rs759655154 113 dbSNP
rs776616566 113 dbSNP
rs1279638670 115 dbSNP
rs1227781891 117 dbSNP
rs1186193361 121 dbSNP
rs1247341146 127 dbSNP
rs1473110059 132 dbSNP
rs1009219402 137 dbSNP
rs1262240831 138 dbSNP
rs753327078 141 dbSNP
rs1398283796 142 dbSNP
rs1407426717 144 dbSNP
rs1411386666 149 dbSNP
rs756805139 150 dbSNP
rs1426306018 156 dbSNP
rs764830010 159 dbSNP
rs1390853614 160 dbSNP
rs1472613659 163 dbSNP
rs919539948 165 dbSNP
rs1320050442 166 dbSNP
rs1189654835 169 dbSNP
rs902852140 176 dbSNP
rs1461856144 179 dbSNP
rs750098617 180 dbSNP
rs143318274 181 dbSNP
rs929543750 182 dbSNP
rs1378936485 183 dbSNP
rs1195989827 185 dbSNP
rs765433413 185 dbSNP
rs758155863 187 dbSNP
rs887748429 188 dbSNP
rs1288577741 189 dbSNP
rs1330859428 195 dbSNP
rs1246266805 197 dbSNP
rs1252937339 198 dbSNP
rs1206253730 202 dbSNP
rs1382901278 203 dbSNP
rs1013009106 207 dbSNP
rs749499318 223 dbSNP
rs768950473 224 dbSNP
rs1402867797 226 dbSNP
rs1013062520 227 dbSNP
rs1405159519 234 dbSNP
rs750209558 247 dbSNP
rs1165230432 260 dbSNP
rs907280693 266 dbSNP
rs1024416528 269 dbSNP
rs774718576 271 dbSNP
rs1437796347 274 dbSNP
rs971655326 275 dbSNP
rs1182199507 283 dbSNP
rs760077992 285 dbSNP
rs1287233148 286 dbSNP
rs1224323945 291 dbSNP
rs771553658 305 dbSNP
rs983437198 313 dbSNP
rs885451 314 dbSNP
rs772251172 326 dbSNP
rs963045946 330 dbSNP
rs1238578103 333 dbSNP
rs773017644 337 dbSNP
rs1376102046 340 dbSNP
rs1266487369 347 dbSNP
rs974076511 360 dbSNP
rs1489672948 363 dbSNP
rs117178835 370 dbSNP
rs573659909 372 dbSNP
rs1325761527 375 dbSNP
rs760703973 376 dbSNP
rs542392877 378 dbSNP
rs559416357 384 dbSNP
rs1425609068 390 dbSNP
rs139255738 392 dbSNP
rs1465488489 395 dbSNP
rs141639960 397 dbSNP
rs911722798 401 dbSNP
rs1409985444 404 dbSNP
rs1177722497 406 dbSNP
rs944514411 409 dbSNP
rs1477566729 414 dbSNP
rs147075313 416 dbSNP
rs1201092269 422 dbSNP
rs1490424458 426 dbSNP
rs565138942 428 dbSNP
rs1262044195 441 dbSNP
rs1213770696 443 dbSNP
rs1318206115 447 dbSNP
rs181056420 448 dbSNP
rs1000289376 456 dbSNP
rs1343312506 457 dbSNP
rs1370075139 458 dbSNP
rs550193523 460 dbSNP
rs1405756796 461 dbSNP
rs776358163 465 dbSNP
rs41274871 472 dbSNP
rs1012611811 475 dbSNP
rs929576388 485 dbSNP
rs1024467475 488 dbSNP
rs1422770429 489 dbSNP
rs529370593 492 dbSNP
rs1480657037 493 dbSNP
rs1422382392 495 dbSNP
rs1004355534 509 dbSNP
rs1486556174 523 dbSNP
rs1016276769 528 dbSNP
rs1242266825 529 dbSNP
rs1436825081 533 dbSNP
rs962973099 541 dbSNP
rs544727559 543 dbSNP
rs974096665 551 dbSNP
rs1317701462 554 dbSNP
rs1276526100 560 dbSNP
rs1245985343 563 dbSNP
rs1028318254 565 dbSNP
rs1302380664 566 dbSNP
rs549173768 568 dbSNP
rs562406314 572 dbSNP
rs986883349 585 dbSNP
rs138273629 586 dbSNP
rs752363852 588 dbSNP
rs938729827 588 dbSNP
rs1056329906 592 dbSNP
rs1168094075 604 dbSNP
rs1429977437 605 dbSNP
rs1419585730 607 dbSNP
rs977260115 608 dbSNP
rs924548359 611 dbSNP
rs757836407 612 dbSNP
rs1054084951 613 dbSNP
rs1206862803 614 dbSNP
rs1361455582 628 dbSNP
rs1308246870 630 dbSNP
rs1009494721 632 dbSNP
rs1241451846 633 dbSNP
rs534842154 634 dbSNP
rs763729483 645 dbSNP
rs948351726 647 dbSNP
rs1258234716 654 dbSNP
rs1399241115 655 dbSNP
rs1045469760 659 dbSNP
rs1463352721 667 dbSNP
rs186297201 669 dbSNP
rs1158184777 672 dbSNP
rs1163642867 682 dbSNP
rs1412419837 692 dbSNP
rs1458565052 693 dbSNP
rs191540392 699 dbSNP
rs1017253772 709 dbSNP
rs1184718013 713 dbSNP
rs965787595 725 dbSNP
rs1236905238 726 dbSNP
rs1004810910 728 dbSNP
rs1265021751 732 dbSNP
rs1219720207 733 dbSNP
rs1015749079 741 dbSNP
rs9608 748 dbSNP
rs1375998289 751 dbSNP
rs1304828342 752 dbSNP
rs1304049047 753 dbSNP
rs1382049499 754 dbSNP
rs1390156065 756 dbSNP
rs1306847971 762 dbSNP
rs950845907 766 dbSNP
rs556901799 769 dbSNP
rs1292577282 777 dbSNP
rs1402760674 785 dbSNP
rs1230581630 793 dbSNP
rs1163614071 795 dbSNP
rs984920107 797 dbSNP
rs1418592479 811 dbSNP
rs1161927924 813 dbSNP
rs182779858 819 dbSNP
rs1056608128 822 dbSNP
rs1261519562 826 dbSNP
rs1420033494 827 dbSNP
rs187429377 833 dbSNP
rs909486543 870 dbSNP
rs995752864 879 dbSNP
rs1028538924 884 dbSNP
rs117965994 885 dbSNP
rs986772200 888 dbSNP
rs1020136550 889 dbSNP
rs1210958867 890 dbSNP
rs1215935370 914 dbSNP
rs966803589 915 dbSNP
rs1241176839 917 dbSNP
rs1479650433 926 dbSNP
rs1176563447 928 dbSNP
rs749630955 941 dbSNP
rs572986461 947 dbSNP
rs1473467244 952 dbSNP
rs924461423 954 dbSNP
rs1346955925 966 dbSNP
rs545212242 968 dbSNP
rs1196992174 976 dbSNP
rs1428216773 980 dbSNP
rs938761110 982 dbSNP
rs1052641 987 dbSNP
rs1414678569 988 dbSNP
rs1382211086 989 dbSNP
rs1177679687 990 dbSNP
rs935957199 994 dbSNP
rs1352279025 996 dbSNP
rs990029271 999 dbSNP
rs755406694 1001 dbSNP
rs1053159872 1002 dbSNP
rs891882813 1010 dbSNP
rs945226523 1029 dbSNP
rs867706133 1037 dbSNP
rs1412117246 1045 dbSNP
rs1281735239 1046 dbSNP
rs565119663 1054 dbSNP
rs1045354322 1062 dbSNP
rs11555365 1081 dbSNP
rs575585834 1086 dbSNP
rs906974502 1090 dbSNP
rs1206798610 1091 dbSNP
rs1017703295 1094 dbSNP
rs779263455 1095 dbSNP
rs1221385094 1105 dbSNP
rs1280931245 1109 dbSNP
rs748445572 1110 dbSNP
rs1283506584 1111 dbSNP
rs901485179 1111 dbSNP
rs997225907 1121 dbSNP
rs1324144497 1144 dbSNP
rs1037643519 1157 dbSNP
rs1382324090 1163 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HeLa
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in Chi_124A_2A8_130_50. RNA binding protein: AGO. Condition:HeLa cell miR-124 + A ...

- Chi SW; Zang JB; Mele A; Darnell RB, 2009, Nature.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' cgguucccuuaccgUCCCuuua 5'
                        ||||    
Target 5' -------------aAGGGcuuu 3'
1 - 9
Article - Chi SW; Zang JB; Mele A; Darnell RB
- Nature, 2009
MicroRNAs (miRNAs) have critical roles in the regulation of gene expression; however, as miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA, predicting target mRNAs is a major challenge. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein-RNA interaction sites. Here we use HITS-CLIP to covalently crosslink native argonaute (Ago, also called Eif2c) protein-RNA complexes in mouse brain. This produced two simultaneous data sets-Ago-miRNA and Ago-mRNA binding sites-that were combined with bioinformatic analysis to identify interaction sites between miRNA and target mRNA. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Ago HITS-CLIP provides a general platform for exploring the specificity and range of miRNA action in vivo, and identifies precise sequences for targeting clinically relevant miRNA-mRNA interactions.
LinkOut: [PMID: 19536157]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084064. RNA binding protein: AGO2. Condition:CLIP_noemetine_AbnovaAb HITS-CLIP data was present in GSM1084065. RNA binding protein: AGO2. Condition:CLIP_emetine_AbnovaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM4903825
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / PID14_NS
Location of target site NM_012266 | 3UTR | AUUAAGGCUAAGAGGUGGGGGACAGGGCCCAUCUACCAGCCUUCAUCAGGAAGGGAAAGGGCUUUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161237
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM4903828
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / PID21_9124
Location of target site NM_001135004 | 3UTR | AGGAAGGGAAAGGGCUUUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161237
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM4903833
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / CTL_TD_21_a
Location of target site NM_001135005 | 3UTR | CCCUGCUGCUCUCAGGAGUGUUCAAGGGUGGAGGGCAGGGAAUGGGGCCUGAGGUAUUAAGGCUAAGAGGUGGGGGACAGGGCCCAUCUACCAGCCUUCAUCAGGAAGGGAAAGGGCUUUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM4903834
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / CTL_TD_21_b
Location of target site NM_001135005 | 3UTR | UCAGGAGUGUUCAAGGGUGGAGGGCAGGGAAUGGGGCCUGAGGUAUUAAGGCUAAGAGGUGGGGGACAGGGCCCAUCUACCAGCCUUCAUCAGGAAGGGAAAGGGCUUUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 5 for dataset GSM4903835
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / CTL_TD_21_c
Location of target site NM_001135005 | 3UTR | CAAGGGUGGAGGGCAGGGAAUGGGGCCUGAGGUAUUAAGGCUAAGAGGUGGGGGACAGGGCCCAUCUACCAGCCUUCAUCAGGAAGGGAAAGGGCUUUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 6 for dataset GSM4903836
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / 124_TD_21_a
Location of target site NM_001135005 | 3UTR | CUACCAGCCUUCAUCAGGAAGGGAAAGGGCUUUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 7 for dataset GSM4903837
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / 124_TD_21_b
Location of target site NM_001135004 | 3UTR | GGAAUGGGGCCUGAGGUAUUAAGGCUAAGAGGUGGGGGACAGGGCCCAUCUACCAGCCUUCAUCAGGAAGGGAAAGGGCUUUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 8 for dataset GSM4903838
Method / RBP HITS-CLIP / AGO
Cell line / Condition Dermal fibroblasts / 124_TD_21_c
Location of target site NM_012266 | 3UTR | GGGCAGGGAAUGGGGCCUGAGGUAUUAAGGCUAAGAGGUGGGGGACAGGGCCCAUCUACCAGCCUUCAUCAGGAAGGGAAAGGGCUUUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161239
CLIP-seq Viewer Link
CLIP-seq Support 9 for dataset Chi_124A_2A8_130_50
Method / RBP HITS-CLIP / AGO
Cell line / Condition HeLa / HeLa cell miR-124 + A
Location of target site ENST00000545841.1 | 3UTR | AAGGGCUUUGGGGUC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 19536157 / Chi_HITSCLIP
CLIP-seq Viewer Link
CLIP-seq Support 10 for dataset GSM1084064
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_noemetine_AbnovaAb
Location of target site ENST00000545841.1 | 3UTR | GAAGGGAAAGGGCUUUGGGGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
CLIP-seq Support 11 for dataset GSM1084065
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_emetine_AbnovaAb
Location of target site ENST00000545841.1 | 3UTR | GAAAGGGCUUUGGGGUC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
223 hsa-miR-4446-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT078060 PCTP phosphatidylcholine transfer protein 2 2
MIRT099072 FOXC1 forkhead box C1 2 2
MIRT130186 TXNIP thioredoxin interacting protein 2 2
MIRT148786 NARS asparaginyl-tRNA synthetase 2 2
MIRT261965 SEPHS1 selenophosphate synthetase 1 2 2
MIRT345867 SRSF2 serine and arginine rich splicing factor 2 2 2
MIRT409087 HNRNPA2B1 heterogeneous nuclear ribonucleoprotein A2/B1 2 2
MIRT451883 SOD2 superoxide dismutase 2 2 4
MIRT469236 RHOB ras homolog family member B 2 2
MIRT469435 REL REL proto-oncogene, NF-kB subunit 2 2
MIRT483055 FOXB1 forkhead box B1 2 8
MIRT484640 TBC1D5 TBC1 domain family member 5 2 6
MIRT493106 MKNK2 MAP kinase interacting serine/threonine kinase 2 2 6
MIRT497307 TMEFF2 transmembrane protein with EGF like and two follistatin like domains 2 2 2
MIRT497798 GABRB1 gamma-aminobutyric acid type A receptor beta1 subunit 2 2
MIRT501273 SCARB2 scavenger receptor class B member 2 2 4
MIRT506756 KMT2D lysine methyltransferase 2D 2 2
MIRT511986 EEF2 eukaryotic translation elongation factor 2 2 4
MIRT513126 ZNF431 zinc finger protein 431 2 2
MIRT519733 ZNF394 zinc finger protein 394 2 4
MIRT520351 UBE2K ubiquitin conjugating enzyme E2 K 2 4
MIRT520542 TPPP tubulin polymerization promoting protein 2 8
MIRT530782 HDHD2 haloacid dehalogenase like hydrolase domain containing 2 2 2
MIRT531112 ZYG11B zyg-11 family member B, cell cycle regulator 2 2
MIRT532144 GALNT8 polypeptide N-acetylgalactosaminyltransferase 8 2 2
MIRT533208 WAPAL WAPL cohesin release factor 2 2
MIRT533419 TWF1 twinfilin actin binding protein 1 2 2
MIRT534063 SRSF10 serine and arginine rich splicing factor 10 2 2
MIRT534288 SLAIN2 SLAIN motif family member 2 2 2
MIRT537796 EFNB2 ephrin B2 2 2
MIRT538017 DNAJC10 DnaJ heat shock protein family (Hsp40) member C10 2 2
MIRT538058 DMD dystrophin 2 2
MIRT538132 DDI2 DNA damage inducible 1 homolog 2 2 2
MIRT538680 CCDC80 coiled-coil domain containing 80 2 2
MIRT538788 C3orf52 chromosome 3 open reading frame 52 2 2
MIRT542762 PPAP2B phospholipid phosphatase 3 2 2
MIRT546861 RAB10 RAB10, member RAS oncogene family 2 4
MIRT547418 MED4 mediator complex subunit 4 2 4
MIRT551633 TRUB1 TruB pseudouridine synthase family member 1 2 4
MIRT552329 ZNF704 zinc finger protein 704 2 2
MIRT556124 MFSD9 major facilitator superfamily domain containing 9 2 4
MIRT559290 ATXN1 ataxin 1 2 2
MIRT562125 IGFBP5 insulin like growth factor binding protein 5 2 2
MIRT563777 HUWE1 HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase 2 2
MIRT564430 EIF2S3 eukaryotic translation initiation factor 2 subunit gamma 2 2
MIRT566102 RBPJ recombination signal binding protein for immunoglobulin kappa J region 2 2
MIRT567126 IRF2BP2 interferon regulatory factor 2 binding protein 2 2 2
MIRT571213 RRS1 ribosome biogenesis regulator homolog 2 2
MIRT571939 LCLAT1 lysocardiolipin acyltransferase 1 2 2
MIRT571967 KIF21A kinesin family member 21A 2 2
MIRT606792 IL1RAPL1 interleukin 1 receptor accessory protein like 1 2 8
MIRT607351 TNS1 tensin 1 2 4
MIRT609416 GJB7 gap junction protein beta 7 2 4
MIRT609522 AZI2 5-azacytidine induced 2 2 4
MIRT611218 FAM174B family with sequence similarity 174 member B 2 2
MIRT611385 TRIP10 thyroid hormone receptor interactor 10 2 2
MIRT612053 KLB klotho beta 2 4
MIRT612102 CHRM3 cholinergic receptor muscarinic 3 2 2
MIRT612146 SIX1 SIX homeobox 1 2 4
MIRT612488 SIX3 SIX homeobox 3 2 4
MIRT613685 QPRT quinolinate phosphoribosyltransferase 2 2
MIRT613763 TTC38 tetratricopeptide repeat domain 38 2 2
MIRT613876 FGD1 FYVE, RhoGEF and PH domain containing 1 2 2
MIRT613965 PPP1R3D protein phosphatase 1 regulatory subunit 3D 2 4
MIRT614894 PAPOLG poly(A) polymerase gamma 2 2
MIRT614912 NFIA nuclear factor I A 2 2
MIRT615013 ERGIC2 ERGIC and golgi 2 2 2
MIRT615106 BCL7A BCL tumor suppressor 7A 2 2
MIRT615242 FAM227A family with sequence similarity 227 member A 2 4
MIRT615695 NEGR1 neuronal growth regulator 1 2 2
MIRT615956 ERBB3 erb-b2 receptor tyrosine kinase 3 2 4
MIRT615979 FSTL4 follistatin like 4 2 2
MIRT616053 PTPRE protein tyrosine phosphatase, receptor type E 2 4
MIRT616073 TBX2 T-box 2 2 4
MIRT616234 NPAS3 neuronal PAS domain protein 3 2 2
MIRT616306 CELF2 CUGBP Elav-like family member 2 2 2
MIRT616474 MACC1 MACC1, MET transcriptional regulator 2 2
MIRT616662 ST3GAL1 ST3 beta-galactoside alpha-2,3-sialyltransferase 1 2 2
MIRT616887 ATP5E ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit 2 2
MIRT616902 LINC00598 long intergenic non-protein coding RNA 598 2 2
MIRT617025 SYT6 synaptotagmin 6 2 2
MIRT617162 SLC16A5 solute carrier family 16 member 5 2 2
MIRT617318 DPF3 double PHD fingers 3 2 2
MIRT617792 CHRM2 cholinergic receptor muscarinic 2 2 2
MIRT618418 DNAJC30 DnaJ heat shock protein family (Hsp40) member C30 2 2
MIRT618663 RPP40 ribonuclease P/MRP subunit p40 2 2
MIRT620212 VN1R1 vomeronasal 1 receptor 1 2 2
MIRT620413 TFDP3 transcription factor Dp family member 3 2 2
MIRT620667 BBS5 Bardet-Biedl syndrome 5 2 2
MIRT620709 ASB16 ankyrin repeat and SOCS box containing 16 2 2
MIRT620956 SFT2D2 SFT2 domain containing 2 2 2
MIRT621225 LMAN1 lectin, mannose binding 1 2 2
MIRT621333 SLC11A1 solute carrier family 11 member 1 2 2
MIRT621696 TSKU tsukushi, small leucine rich proteoglycan 2 2
MIRT623484 KCTD11 potassium channel tetramerization domain containing 11 2 2
MIRT623777 GOSR1 golgi SNAP receptor complex member 1 2 2
MIRT624215 DCAF5 DDB1 and CUL4 associated factor 5 2 2
MIRT624675 ARAP2 ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2 2 2
MIRT627369 PRICKLE4 prickle planar cell polarity protein 4 2 2
MIRT628252 EFCAB14 EF-hand calcium binding domain 14 2 2
MIRT630790 TGIF2 TGFB induced factor homeobox 2 2 2
MIRT635631 PRR15L proline rich 15 like 2 2
MIRT636425 MARCH1 membrane associated ring-CH-type finger 1 2 2
MIRT637295 ACTN2 actinin alpha 2 2 2
MIRT637345 PIGP phosphatidylinositol glycan anchor biosynthesis class P 2 2
MIRT637842 SLC2A9 solute carrier family 2 member 9 2 2
MIRT638327 RCAN1 regulator of calcineurin 1 2 2
MIRT638375 RABL3 RAB, member of RAS oncogene family like 3 2 2
MIRT638751 EPHA4 EPH receptor A4 2 2
MIRT638965 ARHGAP6 Rho GTPase activating protein 6 2 2
MIRT639094 GLIPR1L2 GLI pathogenesis related 1 like 2 2 2
MIRT639172 CEP70 centrosomal protein 70 2 2
MIRT639254 SLC38A1 solute carrier family 38 member 1 2 4
MIRT639368 ZCCHC24 zinc finger CCHC-type containing 24 2 4
MIRT639504 CACNA1G calcium voltage-gated channel subunit alpha1 G 2 2
MIRT639644 WHAMM WAS protein homolog associated with actin, golgi membranes and microtubules 2 2
MIRT640486 EXOC5 exocyst complex component 5 2 2
MIRT641424 SCUBE3 signal peptide, CUB domain and EGF like domain containing 3 2 2
MIRT641550 LIPG lipase G, endothelial type 2 2
MIRT642180 TOR1AIP1 torsin 1A interacting protein 1 2 2
MIRT642656 RGS6 regulator of G protein signaling 6 2 2
MIRT642873 SAMD1 sterile alpha motif domain containing 1 2 2
MIRT643355 TRIM10 tripartite motif containing 10 2 2
MIRT643531 DNTTIP2 deoxynucleotidyltransferase terminal interacting protein 2 2 2
MIRT643998 PPP1R3G protein phosphatase 1 regulatory subunit 3G 2 2
MIRT644105 PHLPP1 PH domain and leucine rich repeat protein phosphatase 1 2 2
MIRT644421 VDR vitamin D receptor 2 2
MIRT644481 SLFN12 schlafen family member 12 2 2
MIRT644573 SPOP speckle type BTB/POZ protein 2 2
MIRT645354 SPNS1 sphingolipid transporter 1 (putative) 2 2
MIRT645383 FBLIM1 filamin binding LIM protein 1 2 2
MIRT645758 SURF6 surfeit 6 2 2
MIRT646060 VANGL2 VANGL planar cell polarity protein 2 2 2
MIRT646194 DUSP10 dual specificity phosphatase 10 2 2
MIRT647915 RGS5 regulator of G protein signaling 5 2 2
MIRT649216 AMMECR1L AMMECR1 like 2 2
MIRT649265 C17orf64 chromosome 17 open reading frame 64 2 2
MIRT649522 GTF3C3 general transcription factor IIIC subunit 3 2 2
MIRT649605 ITPKC inositol-trisphosphate 3-kinase C 2 2
MIRT650392 ORMDL2 ORMDL sphingolipid biosynthesis regulator 2 2 2
MIRT650835 SEMA4G semaphorin 4G 2 2
MIRT651273 ZDHHC5 zinc finger DHHC-type containing 5 2 2
MIRT651674 VPS37A VPS37A, ESCRT-I subunit 2 2
MIRT651763 VASP vasodilator stimulated phosphoprotein 2 2
MIRT652250 TPD52L3 tumor protein D52 like 3 2 2
MIRT652277 TOM1L2 target of myb1 like 2 membrane trafficking protein 2 2
MIRT652316 TNFSF15 TNF superfamily member 15 2 2
MIRT652342 TMOD3 tropomodulin 3 2 2
MIRT652373 TMEM57 transmembrane protein 57 2 2
MIRT652438 TMEM236 transmembrane protein 236 2 2
MIRT652723 TGFB2 transforming growth factor beta 2 2 2
MIRT652752 TET3 tet methylcytosine dioxygenase 3 2 2
MIRT653310 SMOC1 SPARC related modular calcium binding 1 2 2
MIRT653327 SMIM18 small integral membrane protein 18 2 2
MIRT653406 SLC7A2 solute carrier family 7 member 2 2 2
MIRT653512 SLC41A1 solute carrier family 41 member 1 2 2
MIRT653912 SERPINC1 serpin family C member 1 2 2
MIRT654111 RPS6KA5 ribosomal protein S6 kinase A5 2 2
MIRT654153 RORB RAR related orphan receptor B 2 2
MIRT654548 RAB14 RAB14, member RAS oncogene family 2 2
MIRT654920 POLR3D RNA polymerase III subunit D 2 2
MIRT655169 PHF19 PHD finger protein 19 2 2
MIRT655412 PAN2 PAN2 poly(A) specific ribonuclease subunit 2 2
MIRT655784 NOVA2 NOVA alternative splicing regulator 2 2 2
MIRT655836 NGDN neuroguidin 2 2
MIRT656079 MTA3 metastasis associated 1 family member 3 2 2
MIRT656404 MCTP1 multiple C2 and transmembrane domain containing 1 2 2
MIRT656720 LMLN leishmanolysin like peptidase 2 2
MIRT656774 LARP1 La ribonucleoprotein domain family member 1 2 2
MIRT656922 KIAA1462 junctional cadherin 5 associated 2 2
MIRT657004 KCNMB4 potassium calcium-activated channel subfamily M regulatory beta subunit 4 2 2
MIRT657477 HCAR2 hydroxycarboxylic acid receptor 2 2 2
MIRT657539 GSTO2 glutathione S-transferase omega 2 2 2
MIRT657911 GCC1 GRIP and coiled-coil domain containing 1 2 2
MIRT658478 EXOC8 exocyst complex component 8 2 2
MIRT658612 ENPP5 ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative) 2 2
MIRT658823 SRCAP Snf2 related CREBBP activator protein 2 2
MIRT658965 DNAJB5 DnaJ heat shock protein family (Hsp40) member B5 2 4
MIRT659706 CCDC93 coiled-coil domain containing 93 2 2
MIRT660034 C15orf61 chromosome 15 open reading frame 61 2 2
MIRT660108 BTBD3 BTB domain containing 3 2 2
MIRT660697 AMOTL2 angiomotin like 2 2 2
MIRT660763 ALDH6A1 aldehyde dehydrogenase 6 family member A1 2 2
MIRT660884 ADCYAP1R1 ADCYAP receptor type I 2 2
MIRT660956 ABL2 ABL proto-oncogene 2, non-receptor tyrosine kinase 2 2
MIRT661217 SCIMP SLP adaptor and CSK interacting membrane protein 2 2
MIRT661596 C2orf15 chromosome 2 open reading frame 15 2 2
MIRT661624 UGT2B28 UDP glucuronosyltransferase family 2 member B28 2 2
MIRT662430 EID2 EP300 interacting inhibitor of differentiation 2 2 2
MIRT664816 NOX5 NADPH oxidase 5 2 2
MIRT665548 UCHL5 ubiquitin C-terminal hydrolase L5 2 2
MIRT665815 TMEM161B transmembrane protein 161B 2 2
MIRT666668 RBM23 RNA binding motif protein 23 2 2
MIRT667995 HCFC2 host cell factor C2 2 2
MIRT668064 GPR180 G protein-coupled receptor 180 2 2
MIRT669056 CELSR3 cadherin EGF LAG seven-pass G-type receptor 3 2 2
MIRT669637 ACSBG1 acyl-CoA synthetase bubblegum family member 1 2 2
MIRT699368 SLC30A6 solute carrier family 30 member 6 2 2
MIRT699882 RUNX1 runt related transcription factor 1 2 2
MIRT703834 ETV3 ETS variant 3 2 2
MIRT708528 ZNF177 zinc finger protein 177 2 2
MIRT709696 DMWD DM1 locus, WD repeat containing 2 2
MIRT710381 PARD6G par-6 family cell polarity regulator gamma 2 2
MIRT710639 GLUL glutamate-ammonia ligase 2 2
MIRT711584 SETD1A SET domain containing 1A 2 2
MIRT713208 FAM13A family with sequence similarity 13 member A 2 2
MIRT714129 IL20RB interleukin 20 receptor subunit beta 2 2
MIRT714204 MRE11A MRE11 homolog, double strand break repair nuclease 2 2
MIRT714733 CCNO cyclin O 2 2
MIRT714761 ZNF462 zinc finger protein 462 2 2
MIRT715624 ZBTB8B zinc finger and BTB domain containing 8B 2 2
MIRT715755 SKA2 spindle and kinetochore associated complex subunit 2 2 2
MIRT716075 RNF150 ring finger protein 150 2 2
MIRT716179 MTRNR2L1 MT-RNR2-like 1 2 2
MIRT717450 RWDD2A RWD domain containing 2A 2 2
MIRT717866 CACNA2D1 calcium voltage-gated channel auxiliary subunit alpha2delta 1 2 2
MIRT720964 ZBTB43 zinc finger and BTB domain containing 43 2 2
MIRT721564 SLC5A12 solute carrier family 5 member 12 2 2
MIRT722348 BAG2 BCL2 associated athanogene 2 2 2
MIRT723235 BTLA B and T lymphocyte associated 2 2
MIRT724018 LMBRD2 LMBR1 domain containing 2 2 2
MIRT724507 KLHL5 kelch like family member 5 2 2
MIRT725478 GPR26 G protein-coupled receptor 26 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-4446 Aed Therapy sensitive High Epilepsy tissue
hsa-mir-4446 Ceritinib 57379345 NSC776422 approved sensitive High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-mir-4446 Androstenedione+Letrozole sensitive cell line (MCF-7)
hsa-mir-4446 Tamoxifen 2733525 NSC180973 approved resistant cell line (MCF7)
hsa-mir-4446 Cisplatin 5460033 NSC119875 approved resistant cell line (BxPC3)
hsa-mir-4446 Ceritinib 57379345 NSC776422 approved sensitive cell line (H3122)
hsa-miR-4446-5p Cisplatin 5460033 NSC119875 approved resistant cell line (A549)
hsa-miR-4446-5p Paclitaxel 36314 NSC125973 approved resistant cell line (A2780)
hsa-miR-4446-5p Neoadjuvant chemotherapy resistant tissue (breast cancer)
hsa-miR-4446-5p Ceritinib 57379345 NSC776422 approved sensitive cell line (H3122)

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