pre-miRNA Information
pre-miRNA hsa-mir-1245a   
Genomic Coordinates chr2: 188978092 - 188978161
Description Homo sapiens miR-1245a stem-loop
Comment None
RNA Secondary Structure
Associated Diseases

Mature miRNA Information
Mature miRNA hsa-miR-1245a
Sequence 45| AAGUGAUCUAAAGGCCUACAU |65
Evidence Experimental
Experiments Illumina
DRVs in miRNA
Mutant ID Mutant Position Mutant Source
COSN31562560 15 COSMIC
COSN26581044 19 COSMIC
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs906938680 2 dbSNP
rs1159117523 3 dbSNP
rs780486044 6 dbSNP
rs771479866 17 dbSNP
rs1002976932 18 dbSNP
rs1428029219 19 dbSNP
Putative Targets

Gene Information
Gene Symbol DARS2   
Synonyms ASPRS, LBSL, MT-ASPRS
Description aspartyl-tRNA synthetase 2, mitochondrial
Transcript NM_018122   
Expression
Putative miRNA Targets on DARS2
3'UTR of DARS2
(miRNA target sites are highlighted)
>DARS2|NM_018122|3'UTR
   1 ATCATGCATACCATGCAGAAAGTTGAGCTTTTAGGTTTTGTCCTCTTTGCTTCCCCAAGGCTAAAGTCAGATCTAGAGTT
  81 CTGCCACAGGTCTAACAATCAAGTCTTTAGATGGAAGGAATCCAGGCAACATTCTTCACCACAACGAAGAAACAGATAAA
 161 AGATACCCAATTTTGACTTGATTTCATGCATCATTTGGATTTTTTTTGGTTAGGACTTTTTTTGAAGTTCCTTTTTACTT
 241 AGGTGTGAAAGATGGTTCTTTGTTGAAATAATATAGTGGTTTAGTGTTTTCAAATCATGTTTCTCATACCCAGATAGTAG
 321 ATTATTCACTTAGGACAGAGGTAATCAAATTATGTGTGAAATGTAGGAAAATGCTTGCCCCTGTAAACTAGTGAGTTGAT
 401 GGAGCATTTGCTTCATCATCCTCATCAAGAGAATCATATAAATTAAGCTTTATAATGACATTTCAACCATCAACATAATA
 481 TAGTGAGGAGTAGCATAATATTTTTTAATAATGCAGAAAACATCACTGAAATGAGAGTCACAAATTTTTCTTCAGTGTTT
 561 CAGCCTGAGTAAGTTACATAAACCTCGCTTAGCCTCCCTTCCTGCTAATGTGTAAAATACATACTTGCCCTGGCTACCTC
 641 ACCGGGCTGTTATTGCTGGAATCAGAGGAGATAACATATATGGAAGATAAAGTGAATAAAAGTACTTTGAAAAACTATAA
 721 AGCATTCCACAAATATGAGATGATGGTATTATCCATCCATAAATAGGTAGATATATCTCTATTTTATAGTTTCAGATTAA
 801 ACAAAACTGATATCAATAGTAAAAGTCATTTTACTTATC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' uacauccggaaAUCUAGUGAa 5'
                     |||||:|:| 
Target 5' tacccagatagTAGATTATTc 3'
307 - 327 118.00 -6.10
2
miRNA  3' uaCAUCCGGAAAUCUAGUGAa 5'
            ||: |::  |:|| ||:| 
Target 5' taGTGAGTTGATGGAGCATTt 3'
389 - 409 107.00 -8.30
3
miRNA  3' uacaUCCGGAAAUCUAgugaa 5'
              | |:||||||||     
Target 5' aatcAAGTCTTTAGATggaag 3'
97 - 117 93.00 -10.42
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
873686 153 ClinVar
293831 169 ClinVar
293832 211 ClinVar
293833 432 ClinVar
873687 588 ClinVar
293834 643 ClinVar
873688 645 ClinVar
293835 654 ClinVar
COSN30110940 47 COSMIC
COSN30485735 76 COSMIC
COSN26027493 81 COSMIC
COSN31565862 81 COSMIC
COSN30510893 83 COSMIC
COSN31557921 106 COSMIC
COSN13493866 128 COSMIC
COSN26434579 147 COSMIC
COSN31563997 185 COSMIC
COSN18714183 203 COSMIC
COSN22609339 503 COSMIC
COSN19460399 587 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs759030417 6 dbSNP
rs1249302028 7 dbSNP
rs1482222045 15 dbSNP
rs778418949 17 dbSNP
rs1412846820 24 dbSNP
rs749994971 25 dbSNP
rs755664477 34 dbSNP
rs776183062 35 dbSNP
rs200858499 42 dbSNP
rs1250144800 45 dbSNP
rs1000780590 55 dbSNP
rs1186693143 56 dbSNP
rs908756396 59 dbSNP
rs944313360 66 dbSNP
rs546699392 90 dbSNP
rs145246788 99 dbSNP
rs1289688488 108 dbSNP
rs184458663 110 dbSNP
rs1340958985 126 dbSNP
rs748208338 145 dbSNP
rs1019884556 146 dbSNP
rs555583349 153 dbSNP
rs550840346 169 dbSNP
rs972902827 172 dbSNP
rs1025226694 180 dbSNP
rs952422471 187 dbSNP
rs1392206513 198 dbSNP
rs1166986029 200 dbSNP
rs1211523802 200 dbSNP
rs1448909344 202 dbSNP
rs1043109952 206 dbSNP
rs9425758 211 dbSNP
rs529812512 213 dbSNP
rs998790940 217 dbSNP
rs760958796 222 dbSNP
rs1205593670 229 dbSNP
rs959907335 231 dbSNP
rs1475863617 232 dbSNP
rs1484252696 237 dbSNP
rs1271990511 238 dbSNP
rs1169806879 243 dbSNP
rs1227260821 245 dbSNP
rs771259782 262 dbSNP
rs1347940155 271 dbSNP
rs537759436 279 dbSNP
rs990972782 288 dbSNP
rs557695965 291 dbSNP
rs1013500521 295 dbSNP
rs1314050216 307 dbSNP
rs12084787 314 dbSNP
rs1298704938 315 dbSNP
rs1377760616 316 dbSNP
rs1368522500 317 dbSNP
rs918085957 319 dbSNP
rs1025334902 330 dbSNP
rs1395762454 334 dbSNP
rs969233837 335 dbSNP
rs1178311885 336 dbSNP
rs1318054446 339 dbSNP
rs1250122648 355 dbSNP
rs777034042 367 dbSNP
rs1485831061 378 dbSNP
rs981024604 381 dbSNP
rs1257410846 389 dbSNP
rs1026709899 391 dbSNP
rs760039025 394 dbSNP
rs765636264 397 dbSNP
rs982411647 401 dbSNP
rs1348384065 408 dbSNP
rs908201992 409 dbSNP
rs1272657850 412 dbSNP
rs577636691 412 dbSNP
rs1346551754 415 dbSNP
rs1376230689 418 dbSNP
rs1234344319 421 dbSNP
rs1358693927 422 dbSNP
rs1160436364 425 dbSNP
rs886045592 432 dbSNP
rs1473889738 445 dbSNP
rs1181823768 447 dbSNP
rs1475754206 448 dbSNP
rs936684812 457 dbSNP
rs1282447334 485 dbSNP
rs1482632875 490 dbSNP
rs1053684745 492 dbSNP
rs943743175 495 dbSNP
rs889687541 496 dbSNP
rs1210511469 497 dbSNP
rs1326906347 503 dbSNP
rs1267443896 511 dbSNP
rs1233622969 525 dbSNP
rs1007233612 536 dbSNP
rs372838932 538 dbSNP
rs901005378 556 dbSNP
rs1204404801 565 dbSNP
rs765746998 567 dbSNP
rs921541431 579 dbSNP
rs1394557071 587 dbSNP
rs567773377 588 dbSNP
rs1025384256 608 dbSNP
rs1233384056 610 dbSNP
rs1289585655 613 dbSNP
rs1043225083 625 dbSNP
rs189330031 638 dbSNP
rs1387032106 640 dbSNP
rs140743566 643 dbSNP
rs574117039 644 dbSNP
rs542846978 645 dbSNP
rs934737223 647 dbSNP
rs1422952222 650 dbSNP
rs1261999109 652 dbSNP
rs886045593 654 dbSNP
rs1053170457 658 dbSNP
rs375732273 662 dbSNP
rs1453909644 671 dbSNP
rs895798339 673 dbSNP
rs1358171699 681 dbSNP
rs1034441714 695 dbSNP
rs959614964 703 dbSNP
rs1174311706 704 dbSNP
rs1263513457 710 dbSNP
rs1242872120 711 dbSNP
rs1063282 719 dbSNP
rs991480699 721 dbSNP
rs1321272782 725 dbSNP
rs1298621755 727 dbSNP
rs1437323064 741 dbSNP
rs918088729 742 dbSNP
rs971337061 745 dbSNP
rs1320451193 746 dbSNP
rs1014697782 747 dbSNP
rs978157865 753 dbSNP
rs1393323580 754 dbSNP
rs1046279739 772 dbSNP
rs1373240299 774 dbSNP
rs924045643 775 dbSNP
rs905125278 782 dbSNP
rs1447099006 792 dbSNP
rs1389065737 799 dbSNP
rs936757075 802 dbSNP
rs1386148921 803 dbSNP
rs1265184609 808 dbSNP
rs546840059 811 dbSNP
rs1321211218 815 dbSNP
rs182319107 818 dbSNP
rs1230956693 825 dbSNP
rs1340127172 830 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084064. RNA binding protein: AGO2. Condition:CLIP_noemetine_AbnovaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' uacaUCCGGAAAUCUAgugaa 5'
              |||:   |:|||     
Target 5' auugAGGUGGGUGGAU----- 3'
3 - 18
Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084064
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_noemetine_AbnovaAb
Location of target site ENST00000361951.4 | 3UTR | AGAUUGAGGUGGGUGGAU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
BLCA -0.92 0.13 -0.500 0.33 3 Click to see details
BRCA 0.632 0.28 0.500 0.33 3 Click to see details
HNSC 0.219 0.32 0.107 0.41 7 Click to see details
UCEC 0.404 0.37 0.500 0.33 3 Click to see details
UCEC 0.404 0.37 0.500 0.33 3 Click to see details
UCEC 0.404 0.37 0.500 0.33 3 Click to see details
UCEC 0.404 0.37 0.500 0.33 3 Click to see details
UCEC 0.404 0.37 0.500 0.33 3 Click to see details
109 hsa-miR-1245a Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT006574 BRCA2 BRCA2, DNA repair associated 2 1
MIRT055432 SHOC2 SHOC2, leucine rich repeat scaffold protein 2 2
MIRT063879 RASSF8 Ras association domain family member 8 2 6
MIRT077025 WIPF2 WAS/WASL interacting protein family member 2 2 2
MIRT095775 GRPEL2 GrpE like 2, mitochondrial 2 10
MIRT099172 MAP3K4 mitogen-activated protein kinase kinase kinase 4 2 2
MIRT175616 OCRL OCRL, inositol polyphosphate-5-phosphatase 2 2
MIRT456518 LYPLAL1 lysophospholipase like 1 2 2
MIRT464457 UGCG UDP-glucose ceramide glucosyltransferase 2 2
MIRT465747 TMPPE transmembrane protein with metallophosphoesterase domain 2 2
MIRT468257 SFXN4 sideroflexin 4 2 2
MIRT469216 RICTOR RPTOR independent companion of MTOR complex 2 2 2
MIRT472045 NPAT nuclear protein, coactivator of histone transcription 2 2
MIRT481795 APEX1 apurinic/apyrimidinic endodeoxyribonuclease 1 2 2
MIRT500154 CREBBP CREB binding protein 2 2
MIRT506514 MSANTD4 Myb/SANT DNA binding domain containing 4 with coiled-coils 2 4
MIRT506915 KBTBD8 kelch repeat and BTB domain containing 8 2 6
MIRT513901 GRB10 growth factor receptor bound protein 10 2 6
MIRT519128 ALDH2 aldehyde dehydrogenase 2 family (mitochondrial) 2 2
MIRT528121 FOXH1 forkhead box H1 2 2
MIRT528942 SFMBT2 Scm like with four mbt domains 2 2 2
MIRT540580 CEP89 centrosomal protein 89 2 2
MIRT545858 ZNF264 zinc finger protein 264 2 4
MIRT547147 PGM3 phosphoglucomutase 3 2 2
MIRT547434 MED4 mediator complex subunit 4 2 2
MIRT554650 ROBO1 roundabout guidance receptor 1 2 2
MIRT558524 CSRNP3 cysteine and serine rich nuclear protein 3 2 2
MIRT559762 ABHD5 abhydrolase domain containing 5 2 2
MIRT560438 GOLGA7B golgin A7 family member B 2 2
MIRT561974 LRRC59 leucine rich repeat containing 59 2 2
MIRT562442 DDIT4 DNA damage inducible transcript 4 2 2
MIRT563489 ZWINT ZW10 interacting kinetochore protein 2 2
MIRT568265 BMP2K BMP2 inducible kinase 2 2
MIRT572522 KIAA0232 KIAA0232 2 2
MIRT612801 LSM14A LSM14A, mRNA processing body assembly factor 2 2
MIRT621533 ZNF507 zinc finger protein 507 2 2
MIRT625205 GSTCD glutathione S-transferase C-terminal domain containing 2 2
MIRT625615 ZNF84 zinc finger protein 84 2 2
MIRT629215 C12orf66 chromosome 12 open reading frame 66 2 2
MIRT629509 AS3MT arsenite methyltransferase 2 2
MIRT629769 STK25 serine/threonine kinase 25 2 2
MIRT630908 GATAD1 GATA zinc finger domain containing 1 2 2
MIRT632508 RAB13 RAB13, member RAS oncogene family 2 2
MIRT636871 ARSE arylsulfatase E (chondrodysplasia punctata 1) 2 2
MIRT637106 CXorf23 BCLAF1 and THRAP3 family member 3 2 2
MIRT637332 FAM9B family with sequence similarity 9 member B 2 2
MIRT637553 CHST6 carbohydrate sulfotransferase 6 2 2
MIRT637639 ZNF431 zinc finger protein 431 2 2
MIRT637836 CACNG8 calcium voltage-gated channel auxiliary subunit gamma 8 2 2
MIRT638326 RNF11 ring finger protein 11 2 2
MIRT638393 RAB11FIP1 RAB11 family interacting protein 1 2 2
MIRT642450 CLUAP1 clusterin associated protein 1 2 2
MIRT642616 APOPT1 apoptogenic 1, mitochondrial 2 2
MIRT643133 FAM71F2 family with sequence similarity 71 member F2 2 2
MIRT644324 NFKBID NFKB inhibitor delta 2 2
MIRT645150 DIS3 DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease 2 2
MIRT645673 ADK adenosine kinase 2 2
MIRT646091 MGST3 microsomal glutathione S-transferase 3 2 2
MIRT646858 SLC35E4 solute carrier family 35 member E4 2 2
MIRT650153 ZNF426 zinc finger protein 426 2 2
MIRT652483 TMEM181 transmembrane protein 181 2 2
MIRT653790 SIRPA signal regulatory protein alpha 2 2
MIRT655526 PAG1 phosphoprotein membrane anchor with glycosphingolipid microdomains 1 2 2
MIRT657077 JPH2 junctophilin 2 2 2
MIRT657322 HOOK3 hook microtubule tethering protein 3 2 2
MIRT662036 FUT2 fucosyltransferase 2 2 2
MIRT662322 ADM2 adrenomedullin 2 2 2
MIRT663211 DARS2 aspartyl-tRNA synthetase 2, mitochondrial 2 2
MIRT663559 CCR6 C-C motif chemokine receptor 6 2 2
MIRT663937 ZNF554 zinc finger protein 554 2 2
MIRT664689 EIF2B2 eukaryotic translation initiation factor 2B subunit beta 2 2
MIRT664709 PAICS phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthase 2 2
MIRT665291 ZFP14 ZFP14 zinc finger protein 2 2
MIRT665572 TXNL1 thioredoxin like 1 2 2
MIRT667068 PAOX polyamine oxidase 2 2
MIRT667304 MYO5A myosin VA 2 2
MIRT667442 METTL14 methyltransferase like 14 2 2
MIRT668048 GTPBP10 GTP binding protein 10 2 2
MIRT669604 AGO3 argonaute 3, RISC catalytic component 2 2
MIRT671088 ABL2 ABL proto-oncogene 2, non-receptor tyrosine kinase 2 2
MIRT674361 SLC35E3 solute carrier family 35 member E3 2 2
MIRT674962 ORAI2 ORAI calcium release-activated calcium modulator 2 2 2
MIRT676394 SEC24D SEC24 homolog D, COPII coat complex component 2 2
MIRT676819 AGMAT agmatinase 2 2
MIRT676891 ENSA endosulfine alpha 2 2
MIRT677059 ZNF34 zinc finger protein 34 2 2
MIRT678424 ANKRD36 ankyrin repeat domain 36 2 2
MIRT678735 SRCAP Snf2 related CREBBP activator protein 2 2
MIRT678930 XPOT exportin for tRNA 2 2
MIRT678954 MYADM myeloid associated differentiation marker 2 2
MIRT679228 MAN2A2 mannosidase alpha class 2A member 2 2 2
MIRT679765 TLR6 toll like receptor 6 2 2
MIRT681992 HRH4 histamine receptor H4 2 2
MIRT687405 NSUN4 NOP2/Sun RNA methyltransferase family member 4 2 2
MIRT694570 RBMXL1 RNA binding motif protein, X-linked like 1 2 2
MIRT698449 TM4SF1 transmembrane 4 L six family member 1 2 2
MIRT706406 HAS2 hyaluronan synthase 2 2 2
MIRT706582 ZNF432 zinc finger protein 432 2 2
MIRT706838 VHLL VHL like 2 2
MIRT706909 DVL3 dishevelled segment polarity protein 3 2 2
MIRT707052 TRPV2 transient receptor potential cation channel subfamily V member 2 2 2
MIRT708583 C11orf54 chromosome 11 open reading frame 54 2 2
MIRT708988 CABP4 calcium binding protein 4 2 2
MIRT713041 ADRA2B adrenoceptor alpha 2B 2 2
MIRT713731 SUCO SUN domain containing ossification factor 2 2
MIRT714968 RAB21 RAB21, member RAS oncogene family 2 2
MIRT717485 PDE4DIP phosphodiesterase 4D interacting protein 2 2
MIRT722826 KLK2 kallikrein related peptidase 2 2 2
MIRT723305 MOGAT1 monoacylglycerol O-acyltransferase 1 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-1245a Trametinib 11707110 NSC758246 approved resistant High Melanoma cell line (M14) (500nM)
hsa-miR-1245a Vemurafenib 42611257 NSC761431 approved resistant High Melanoma cell line (M14) (500nM)
hsa-miR-1245a Trametinib 11707110 NSC758246 approved resistant High Melanoma cell line (M14) (1uM)
hsa-miR-1245a Vemurafenib 42611257 NSC761431 approved resistant High Melanoma cell line (M14) (1uM)
hsa-miR-1245a Cisplatin 5460033 NSC119875 approved sensitive cell line (A2780)
hsa-miR-1245a Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)

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