pre-miRNA Information | |
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pre-miRNA | hsa-mir-6752 |
Genomic Coordinates | chr11: 67490245 - 67490315 |
Description | Homo sapiens miR-6752 stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Mature miRNA Information | ||||||||||||||||
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Mature miRNA | hsa-miR-6752-3p | |||||||||||||||
Sequence | 51| UCCCUGCCCCCAUACUCCCAG |71 | |||||||||||||||
Evidence | Experimental | |||||||||||||||
Experiments | Meta-analysis | |||||||||||||||
SNPs in miRNA |
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Putative Targets |
miRNA Expression profile | |
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miRNAs in Extracellular Vesicles |
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Circulating MicroRNA Expression Profiling |
Gene Information | |||||||||||||||||||||
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Gene Symbol | FBXO2 | ||||||||||||||||||||
Synonyms | FBG1, FBX2, Fbs1, NFB42, OCP1 | ||||||||||||||||||||
Description | F-box protein 2 | ||||||||||||||||||||
Transcript | NM_012168 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on FBXO2 | |||||||||||||||||||||
3'UTR of FBXO2 (miRNA target sites are highlighted) |
>FBXO2|NM_012168|3'UTR 1 GCGACCCTGCCTCCACTCTCCCCCAGCTGCCTGGAGGGGCAAAGGTCGAGGGTAGATAGGCCTTAACTTAGTCCATAGCG 81 TCCTCACCTTCCCCAAGCCACACATCCTCCTCCCATCCCTTGCTCCGATCCCAGCCCCTGAGCAGGGAGAGAGAAGTTTT 161 GTTGGCATAGGTTTGCTTAGGTAGCCGGCTTCTAGAATGTAGATCCGTGAGGGCGTGAGCTTGGACTGGACCCACTGCTG 241 ATTCCCTGCACCTAGCACAGTGCTTGCCACAAAGTGGGCTCTCAATAAATATTATTTGTCCAAGGAAGGAATGAATGAAA 321 AAATGGATAAACAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
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miRNA:Target | ---- | ||||||
Validation Method |
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Conditions | HEK293S | ||||||
Location of target site | 3'UTR | ||||||
Tools used in this research | TargetScan , miRTarCLIP , Piranha | ||||||
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM1084064. RNA binding protein: AGO2. Condition:CLIP_noemetine_AbnovaAb
... - Karginov FV; Hannon GJ, 2013, Genes & development. |
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miRNA-target interactions (Provided by authors) |
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Article |
- Karginov FV; Hannon GJ - Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
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CLIP-seq Support 1 for dataset GSM1084064 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_noemetine_AbnovaAb |
Location of target site | ENST00000354287.4 | 3UTR | AGCAGGGAGAGAGAAGUUUUGUUGGC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
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MiRNA-Target Expression Profile (TCGA) | |||||||
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110 hsa-miR-6752-3p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT152341 | TNFSF9 | TNF superfamily member 9 | ![]() |
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2 | 2 | ||||||
MIRT273594 | SP1 | Sp1 transcription factor | ![]() |
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2 | 2 | ||||||
MIRT291927 | TPM4 | tropomyosin 4 | ![]() |
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2 | 4 | ||||||
MIRT311186 | ANKRD33B | ankyrin repeat domain 33B | ![]() |
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2 | 4 | ||||||
MIRT378521 | CDKN1A | cyclin dependent kinase inhibitor 1A | ![]() |
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2 | 4 | ||||||
MIRT452467 | SORCS2 | sortilin related VPS10 domain containing receptor 2 | ![]() |
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2 | 2 | ||||||
MIRT478636 | CTDNEP1 | CTD nuclear envelope phosphatase 1 | ![]() |
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2 | 2 | ||||||
MIRT496343 | TMEM81 | transmembrane protein 81 | ![]() |
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2 | 2 | ||||||
MIRT496646 | MFAP3 | microfibril associated protein 3 | ![]() |
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2 | 2 | ||||||
MIRT496711 | DNAJC30 | DnaJ heat shock protein family (Hsp40) member C30 | ![]() |
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2 | 2 | ||||||
MIRT499242 | VAV3 | vav guanine nucleotide exchange factor 3 | ![]() |
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2 | 4 | ||||||
MIRT519777 | ZNF354B | zinc finger protein 354B | ![]() |
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2 | 6 | ||||||
MIRT520883 | STX17 | syntaxin 17 | ![]() |
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2 | 2 | ||||||
MIRT522476 | ZAK | mitogen-activated protein kinase kinase kinase 20 | ![]() |
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2 | 2 | ||||||
MIRT523587 | G3BP2 | G3BP stress granule assembly factor 2 | ![]() |
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2 | 6 | ||||||
MIRT525240 | KCNJ12 | potassium voltage-gated channel subfamily J member 12 | ![]() |
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2 | 2 | ||||||
MIRT527025 | PABPN1L | poly(A) binding protein nuclear 1 like, cytoplasmic | ![]() |
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2 | 2 | ||||||
MIRT527709 | IL17REL | interleukin 17 receptor E like | ![]() |
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2 | 2 | ||||||
MIRT530778 | GPD1 | glycerol-3-phosphate dehydrogenase 1 | ![]() |
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2 | 2 | ||||||
MIRT531883 | SCN1B | sodium voltage-gated channel beta subunit 1 | ![]() |
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2 | 2 | ||||||
MIRT533043 | ZBTB46 | zinc finger and BTB domain containing 46 | ![]() |
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2 | 2 | ||||||
MIRT535062 | PPP2R5D | protein phosphatase 2 regulatory subunit B'delta | ![]() |
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2 | 4 | ||||||
MIRT536512 | KCTD15 | potassium channel tetramerization domain containing 15 | ![]() |
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2 | 2 | ||||||
MIRT543793 | RALGAPB | Ral GTPase activating protein non-catalytic beta subunit | ![]() |
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2 | 2 | ||||||
MIRT563977 | HCFC1 | host cell factor C1 | ![]() |
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2 | 2 | ||||||
MIRT569472 | MAN2A2 | mannosidase alpha class 2A member 2 | ![]() |
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2 | 2 | ||||||
MIRT570600 | NFIX | nuclear factor I X | ![]() |
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2 | 2 | ||||||
MIRT571402 | MED4 | mediator complex subunit 4 | ![]() |
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2 | 2 | ||||||
MIRT608418 | PPIC | peptidylprolyl isomerase C | ![]() |
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2 | 6 | ||||||
MIRT608928 | REXO2 | RNA exonuclease 2 | ![]() |
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2 | 4 | ||||||
MIRT609357 | ZNF664 | zinc finger protein 664 | ![]() |
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2 | 2 | ||||||
MIRT611189 | WBSCR17 | polypeptide N-acetylgalactosaminyltransferase 17 | ![]() |
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2 | 4 | ||||||
MIRT615266 | DPF2 | double PHD fingers 2 | ![]() |
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2 | 2 | ||||||
MIRT615408 | VDAC2 | voltage dependent anion channel 2 | ![]() |
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2 | 2 | ||||||
MIRT616323 | AGO3 | argonaute 3, RISC catalytic component | ![]() |
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2 | 2 | ||||||
MIRT616566 | ZNF512B | zinc finger protein 512B | ![]() |
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2 | 2 | ||||||
MIRT616745 | TAZ | tafazzin | ![]() |
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2 | 2 | ||||||
MIRT617316 | MBOAT1 | membrane bound O-acyltransferase domain containing 1 | ![]() |
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2 | 2 | ||||||
MIRT620030 | NFAM1 | NFAT activating protein with ITAM motif 1 | ![]() |
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2 | 2 | ||||||
MIRT621166 | RTTN | rotatin | ![]() |
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2 | 2 | ||||||
MIRT623501 | KCNQ3 | potassium voltage-gated channel subfamily Q member 3 | ![]() |
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2 | 2 | ||||||
MIRT623524 | KCNK10 | potassium two pore domain channel subfamily K member 10 | ![]() |
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2 | 2 | ||||||
MIRT629683 | TNFAIP8L1 | TNF alpha induced protein 8 like 1 | ![]() |
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2 | 2 | ||||||
MIRT630795 | TGIF2 | TGFB induced factor homeobox 2 | ![]() |
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2 | 2 | ||||||
MIRT634638 | HIP1 | huntingtin interacting protein 1 | ![]() |
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2 | 4 | ||||||
MIRT636788 | RAB40B | RAB40B, member RAS oncogene family | ![]() |
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2 | 2 | ||||||
MIRT640332 | DAAM2 | dishevelled associated activator of morphogenesis 2 | ![]() |
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2 | 2 | ||||||
MIRT640557 | SMCR8 | Smith-Magenis syndrome chromosome region, candidate 8 | ![]() |
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2 | 2 | ||||||
MIRT643319 | TMEM151B | transmembrane protein 151B | ![]() |
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2 | 2 | ||||||
MIRT643991 | DUSP28 | dual specificity phosphatase 28 | ![]() |
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2 | 2 | ||||||
MIRT644618 | CD300E | CD300e molecule | ![]() |
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2 | 2 | ||||||
MIRT646445 | XRCC2 | X-ray repair cross complementing 2 | ![]() |
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2 | 2 | ||||||
MIRT648020 | SLCO4C1 | solute carrier organic anion transporter family member 4C1 | ![]() |
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2 | 2 | ||||||
MIRT648077 | ZMIZ2 | zinc finger MIZ-type containing 2 | ![]() |
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2 | 2 | ||||||
MIRT649618 | ITPKC | inositol-trisphosphate 3-kinase C | ![]() |
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2 | 2 | ||||||
MIRT650414 | ZNF442 | zinc finger protein 442 | ![]() |
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2 | 2 | ||||||
MIRT650850 | SEMA4G | semaphorin 4G | ![]() |
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2 | 2 | ||||||
MIRT654694 | PSMB5 | proteasome subunit beta 5 | ![]() |
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2 | 2 | ||||||
MIRT655512 | PAIP2B | poly(A) binding protein interacting protein 2B | ![]() |
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2 | 2 | ||||||
MIRT657620 | GRAP2 | GRB2-related adaptor protein 2 | ![]() |
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2 | 2 | ||||||
MIRT658009 | GALNT10 | polypeptide N-acetylgalactosaminyltransferase 10 | ![]() |
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2 | 2 | ||||||
MIRT658329 | FAM83F | family with sequence similarity 83 member F | ![]() |
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2 | 2 | ||||||
MIRT658832 | SRCAP | Snf2 related CREBBP activator protein | ![]() |
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2 | 2 | ||||||
MIRT658920 | DPY19L4 | dpy-19 like 4 | ![]() |
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2 | 2 | ||||||
MIRT661058 | RPL18A | ribosomal protein L18a | ![]() |
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2 | 2 | ||||||
MIRT662342 | MYLK3 | myosin light chain kinase 3 | ![]() |
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2 | 2 | ||||||
MIRT663451 | FBXO2 | F-box protein 2 | ![]() |
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2 | 2 | ||||||
MIRT676752 | SGTB | small glutamine rich tetratricopeptide repeat containing beta | ![]() |
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2 | 4 | ||||||
MIRT684705 | LRRD1 | leucine rich repeats and death domain containing 1 | ![]() |
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2 | 2 | ||||||
MIRT687247 | PDHB | pyruvate dehydrogenase E1 beta subunit | ![]() |
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2 | 2 | ||||||
MIRT693881 | NT5C2 | 5'-nucleotidase, cytosolic II | ![]() |
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2 | 2 | ||||||
MIRT693922 | HNRNPA1L2 | heterogeneous nuclear ribonucleoprotein A1-like 2 | ![]() |
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2 | 2 | ||||||
MIRT695921 | ZNF174 | zinc finger protein 174 | ![]() |
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2 | 2 | ||||||
MIRT702867 | HNRNPA1 | heterogeneous nuclear ribonucleoprotein A1 | ![]() |
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2 | 2 | ||||||
MIRT708863 | TMSB4X | thymosin beta 4, X-linked | ![]() |
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2 | 2 | ||||||
MIRT708936 | CRY2 | cryptochrome circadian clock 2 | ![]() |
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2 | 2 | ||||||
MIRT709476 | FAXC | failed axon connections homolog | ![]() |
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2 | 2 | ||||||
MIRT709752 | UBD | ubiquitin D | ![]() |
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2 | 2 | ||||||
MIRT710128 | VANGL2 | VANGL planar cell polarity protein 2 | ![]() |
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2 | 2 | ||||||
MIRT710274 | FAM107A | family with sequence similarity 107 member A | ![]() |
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2 | 2 | ||||||
MIRT711146 | TMEM174 | transmembrane protein 174 | ![]() |
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2 | 2 | ||||||
MIRT712582 | ATP2B4 | ATPase plasma membrane Ca2+ transporting 4 | ![]() |
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2 | 2 | ||||||
MIRT712648 | TXNL4A | thioredoxin like 4A | ![]() |
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2 | 2 | ||||||
MIRT713325 | TMEM44 | transmembrane protein 44 | ![]() |
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2 | 2 | ||||||
MIRT713413 | PEX16 | peroxisomal biogenesis factor 16 | ![]() |
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2 | 2 | ||||||
MIRT713891 | RNF19B | ring finger protein 19B | ![]() |
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2 | 2 | ||||||
MIRT714549 | COL14A1 | collagen type XIV alpha 1 chain | ![]() |
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2 | 2 | ||||||
MIRT714809 | ARHGEF19 | Rho guanine nucleotide exchange factor 19 | ![]() |
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2 | 2 | ||||||
MIRT714840 | SCAMP2 | secretory carrier membrane protein 2 | ![]() |
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2 | 2 | ||||||
MIRT715779 | DDX42 | DEAD-box helicase 42 | ![]() |
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2 | 2 | ||||||
MIRT715795 | TBL3 | transducin beta like 3 | ![]() |
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2 | 2 | ||||||
MIRT716088 | RNF150 | ring finger protein 150 | ![]() |
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2 | 2 | ||||||
MIRT716712 | IMP4 | IMP4, U3 small nucleolar ribonucleoprotein | ![]() |
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2 | 2 | ||||||
MIRT716737 | APOL6 | apolipoprotein L6 | ![]() |
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2 | 2 | ||||||
MIRT717069 | MTMR6 | myotubularin related protein 6 | ![]() |
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2 | 2 | ||||||
MIRT718185 | HLCS | holocarboxylase synthetase | ![]() |
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2 | 2 | ||||||
MIRT719386 | SECTM1 | secreted and transmembrane 1 | ![]() |
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2 | 2 | ||||||
MIRT720679 | SLC39A13 | solute carrier family 39 member 13 | ![]() |
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2 | 2 | ||||||
MIRT721315 | FAM58A | cyclin Q | ![]() |
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2 | 2 | ||||||
MIRT721396 | LDLRAD4 | low density lipoprotein receptor class A domain containing 4 | ![]() |
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2 | 2 | ||||||
MIRT721896 | ITPR2 | inositol 1,4,5-trisphosphate receptor type 2 | ![]() |
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2 | 2 | ||||||
MIRT722442 | ZBTB7B | zinc finger and BTB domain containing 7B | ![]() |
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2 | 2 | ||||||
MIRT723009 | FADS1 | fatty acid desaturase 1 | ![]() |
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2 | 2 | ||||||
MIRT723675 | CTC1 | CST telomere replication complex component 1 | ![]() |
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2 | 2 | ||||||
MIRT723774 | ROBO4 | roundabout guidance receptor 4 | ![]() |
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2 | 2 | ||||||
MIRT723814 | OR1L8 | olfactory receptor family 1 subfamily L member 8 | ![]() |
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2 | 2 | ||||||
MIRT723959 | TMEM184A | transmembrane protein 184A | ![]() |
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2 | 2 | ||||||
MIRT724449 | OPA3 | OPA3, outer mitochondrial membrane lipid metabolism regulator | ![]() |
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2 | 2 | ||||||
MIRT724798 | C1D | C1D nuclear receptor corepressor | ![]() |
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2 | 2 | ||||||
MIRT725626 | CAMKV | CaM kinase like vesicle associated | ![]() |
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2 | 2 |