miRTarBase - #MIRT664712


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pre-miRNA Information
pre-miRNA hsa-mir-3200   
Genomic Coordinates chr22: 30731557 - 30731641
Description Homo sapiens miR-3200 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-3200-3p
Sequence 54| CACCUUGCGCUACUCAGGUCUG |75
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1354947096 9 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol EIF4G3   
Synonyms eIF-4G 3, eIF4G 3, eIF4GII
Description eukaryotic translation initiation factor 4 gamma 3
Transcript NM_003760   
Expression
Putative miRNA Targets on EIF4G3
3'UTR of EIF4G3
(miRNA target sites are highlighted)		 
>EIF4G3|NM_003760|3'UTR
   1 AACTTCAAATACACAAAATGAAACAAAAGAAACAATTTAAGTATTTTTTTAAAAAGTTTCACGTCTTCGCCAATCACAGT
  81 GCAGCAAGGCCAATTCTCGCAGAAACCCCCACGTGTGCACGAGTGGGAGAGGGGAAAGAGAAAAAAAGGTGATCATGGAG
 161 GAAAAAGGTACTGGATAAAAGTAAACTTCAAACCTTAGGGCGGGAGCACTAAAACCAAAATACATGTATTATTTATAGAA
 241 AATATTTTCTGTTTTAATCTTTTCTTTTTAAACAAGGACTCATACTTAAAAAAATGTTTAGCAAAAAAAAAAAAAGTTGA
 321 GAACTTTTAATTTATTTTAAGGACTGCAAATGCCAGTGTAATTTTTTAATTTGCAGTTTCTGTAAACAACTTGTATAATA
 401 GAAAAGCAGAGAAATAAATTTCCCTCCCCTTCAAGATGCACCTCATGTTTGTTTTAAGGTATAGCATTTAGTCCAGATTT
 481 GAGAAAGTTTGGGGTGAACAAGGTAAGAAAGATTTTTTTTTTTTTGGCATCAAATCTTTCTGCCTGCCTCTCAGCTTGCT
 561 TCAGAAAATTTAAAAAATCACAATAGTAATCAAAACATACATAACATTGAAACAGAAGGAAATGCTGTGGACCACAGAAC
 641 TCCAAGAATTGTTTAAAAAAAAAAAAGTGCTACCCTGAGAAAAGTACTCTTAATACTCTTGAAATCTTTAGAGCAACTTT
 721 AAGGCTTGTAAATACATAGAACAAATATTTAAAAAAACAAAAAGAAATTGACTCAGTACTATTTCTTTTCACTTTGAAAA
 801 TATAAAGAACAAAATAAAGACAAACATTGCAAGTTTAAAAGAAAGTAAAGTGACTTCTCCTTTGGACAGCTGCTGCATGT
 881 GTGCCCATTCCTGGGGGTGCTGTCTGGCTATTTATTGTCTAATTCAAATCACTCCTGAGGGGAGAGAGATAAAACGAGAG
 961 AGAGTGAGAGAGTGTGTGTATGTGCGTGTATGCGCACGTATGTGCATGCACATGTATGTATGTATAT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' gucUGGACUCAUCGCGUUCCac 5'
             |:|  |||:  ||||||  
Target 5' ccaATCACAGTGCAGCAAGGcc 3'
 70 - 91 135.00 -12.10
2 
miRNA  3' gucUGGACUCAUCGCGUUCCAc 5'
             :::||:|  |  |||||| 
Target 5' aaaGTTTGGGGTGAACAAGGTa 3'
 484 - 505 123.00 -11.30
3 
miRNA  3' guCUGGACUCAUCGCGUUCCAc 5'
            |||   |  | :||||| | 
Target 5' aaGAC--AAACATTGCAAGTTt 3'
 817 - 836 112.00 -8.06
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30144439 19 COSMIC
COSN30144440 20 COSMIC
COSN30128586 32 COSMIC
COSN30109064 50 COSMIC
COSN28662583 60 COSMIC
COSN28880779 63 COSMIC
COSN24396658 69 COSMIC
COSN7201178 73 COSMIC
COSN30449735 98 COSMIC
COSN26439891 113 COSMIC
COSN31582184 147 COSMIC
COSN32120382 161 COSMIC
COSN24297766 166 COSMIC
COSN29004732 302 COSMIC
COSN20094488 303 COSMIC
COSN30164385 319 COSMIC
COSN31606598 324 COSMIC
COSN30174206 401 COSMIC
COSN31569884 421 COSMIC
COSN20094486 512 COSMIC
COSN20094487 513 COSMIC
COSN31585306 536 COSMIC
COSN25721946 615 COSMIC
COSN25752676 623 COSMIC
COSN30538197 654 COSMIC
COSN27412908 655 COSMIC
COSN31524375 750 COSMIC
COSN26572606 776 COSMIC
COSN1429255 827 COSMIC
COSN29317192 850 COSMIC
COSN7201171 949 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs752197358 4 dbSNP
rs780721683 10 dbSNP
rs754443566 12 dbSNP
rs776091222 12 dbSNP
rs202173650 16 dbSNP
rs765956273 19 dbSNP
rs762166851 20 dbSNP
rs1413299529 27 dbSNP
rs1398647861 29 dbSNP
rs772621170 29 dbSNP
rs1168586680 31 dbSNP
rs754223109 33 dbSNP
rs979665832 36 dbSNP
rs764563737 38 dbSNP
rs1185698014 41 dbSNP
rs761226476 48 dbSNP
rs775476606 50 dbSNP
rs746363580 51 dbSNP
rs767740107 51 dbSNP
rs1346689124 60 dbSNP
rs1272622350 62 dbSNP
rs1228918607 63 dbSNP
rs1327550190 66 dbSNP
rs1295419565 68 dbSNP
rs1021312049 73 dbSNP
rs1357826049 74 dbSNP
rs1313852302 78 dbSNP
rs756586385 90 dbSNP
rs1373832343 96 dbSNP
rs1243800481 98 dbSNP
rs375666603 99 dbSNP
rs371811192 109 dbSNP
rs907719117 112 dbSNP
rs1259424206 113 dbSNP
rs1188365178 114 dbSNP
rs766973359 117 dbSNP
rs756923767 121 dbSNP
rs1276006164 122 dbSNP
rs1047148439 125 dbSNP
rs1230266054 126 dbSNP
rs1339395962 128 dbSNP
rs1250260000 134 dbSNP
rs1360288532 138 dbSNP
rs1270023359 140 dbSNP
rs77692149 141 dbSNP
rs1006167053 147 dbSNP
rs1331073828 148 dbSNP
rs1432104157 148 dbSNP
rs887678267 148 dbSNP
rs931392081 148 dbSNP
rs1363071543 154 dbSNP
rs1168607451 171 dbSNP
rs1301840552 172 dbSNP
rs1045106999 192 dbSNP
rs539256501 201 dbSNP
rs1365682205 202 dbSNP
rs991525500 204 dbSNP
rs1166443274 208 dbSNP
rs1183620107 222 dbSNP
rs1426407053 223 dbSNP
rs1418519112 224 dbSNP
rs763701479 230 dbSNP
rs1249261367 232 dbSNP
rs1196170835 235 dbSNP
rs1470243408 237 dbSNP
rs1274303224 238 dbSNP
rs1233075782 239 dbSNP
rs1354610923 251 dbSNP
rs1186944404 255 dbSNP
rs571738690 256 dbSNP
rs928928801 259 dbSNP
rs982001930 262 dbSNP
rs935390910 279 dbSNP
rs1443146526 282 dbSNP
rs548023315 295 dbSNP
rs1266165272 299 dbSNP
rs1326417610 300 dbSNP
rs1224011104 301 dbSNP
rs1306053387 302 dbSNP
rs1272725791 303 dbSNP
rs979549724 303 dbSNP
rs189760416 304 dbSNP
rs1342808244 306 dbSNP
rs1365176096 316 dbSNP
rs200324002 316 dbSNP
rs946997324 317 dbSNP
rs967551121 326 dbSNP
rs911453252 340 dbSNP
rs1404930576 345 dbSNP
rs1020886257 346 dbSNP
rs1011248452 353 dbSNP
rs1384853028 362 dbSNP
rs1460138478 367 dbSNP
rs1353498441 368 dbSNP
rs957122860 381 dbSNP
rs1028614544 382 dbSNP
rs1435728207 384 dbSNP
rs953164612 395 dbSNP
rs1466958620 396 dbSNP
rs922927402 399 dbSNP
rs1426971430 406 dbSNP
rs995833547 429 dbSNP
rs901744811 436 dbSNP
rs1040267838 439 dbSNP
rs1198576822 440 dbSNP
rs1395225721 441 dbSNP
rs1480746743 442 dbSNP
rs961773739 466 dbSNP
rs1014795309 471 dbSNP
rs1194376633 475 dbSNP
rs1446715482 484 dbSNP
rs1284315960 487 dbSNP
rs535341005 489 dbSNP
rs1348839078 499 dbSNP
rs1279751734 500 dbSNP
rs952035662 502 dbSNP
rs1365431062 507 dbSNP
rs886239508 511 dbSNP
rs1193049149 512 dbSNP
rs1012133672 513 dbSNP
rs1339672568 518 dbSNP
rs1048927183 525 dbSNP
rs1296107026 526 dbSNP
rs1412219978 526 dbSNP
rs748803491 526 dbSNP
rs749122429 526 dbSNP
rs1479706763 527 dbSNP
rs1187682874 532 dbSNP
rs1255645342 544 dbSNP
rs1250929788 548 dbSNP
rs1056369624 554 dbSNP
rs931382059 572 dbSNP
rs1190471951 578 dbSNP
rs1486137365 589 dbSNP
rs74522845 597 dbSNP
rs1055788941 599 dbSNP
rs1261703678 600 dbSNP
rs1309264292 601 dbSNP
rs1043904490 607 dbSNP
rs1273800698 613 dbSNP
rs946872202 617 dbSNP
rs1313353371 619 dbSNP
rs1276118918 626 dbSNP
rs1304062219 642 dbSNP
rs1355725373 644 dbSNP
rs1389105749 645 dbSNP
rs1372025810 646 dbSNP
rs1301843265 651 dbSNP
rs1331552298 655 dbSNP
rs1445347622 655 dbSNP
rs1259884961 667 dbSNP
rs1465597751 667 dbSNP
rs532703823 667 dbSNP
rs1448602735 668 dbSNP
rs1167578772 672 dbSNP
rs1403598953 676 dbSNP
rs1175525635 679 dbSNP
rs1470242643 684 dbSNP
rs1379240381 685 dbSNP
rs549082543 691 dbSNP
rs1156843969 692 dbSNP
rs1417344926 696 dbSNP
rs1481733714 704 dbSNP
rs1233573953 724 dbSNP
rs937380111 725 dbSNP
rs1179460837 736 dbSNP
rs928647366 738 dbSNP
rs530545337 743 dbSNP
rs981690825 751 dbSNP
rs376512912 758 dbSNP
rs1335309298 762 dbSNP
rs1311726951 764 dbSNP
rs934230146 766 dbSNP
rs1241666218 780 dbSNP
rs922954131 781 dbSNP
rs1445336524 785 dbSNP
rs1328433511 815 dbSNP
rs1398753149 815 dbSNP
rs975733785 828 dbSNP
rs1163097592 829 dbSNP
rs968044211 835 dbSNP
rs953419188 841 dbSNP
rs1408940848 845 dbSNP
rs961824501 864 dbSNP
rs907622888 867 dbSNP
rs913364632 868 dbSNP
rs990319313 869 dbSNP
rs1215544797 877 dbSNP
rs1203365097 878 dbSNP
rs1264676577 892 dbSNP
rs563263591 895 dbSNP
rs984502062 897 dbSNP
rs1309457095 908 dbSNP
rs957111587 913 dbSNP
rs1374105674 926 dbSNP
rs1258240303 931 dbSNP
rs34345098 943 dbSNP
rs1028729102 950 dbSNP
rs1315794173 955 dbSNP
rs144569614 956 dbSNP
rs140906103 962 dbSNP
rs1455628466 964 dbSNP
rs1019034502 970 dbSNP
rs1158208365 971 dbSNP
rs759481461 976 dbSNP
rs1418082203 978 dbSNP
rs1194300780 980 dbSNP
rs960636672 980 dbSNP
rs1433728439 985 dbSNP
rs1266527497 986 dbSNP
rs559222359 988 dbSNP
rs1380197313 993 dbSNP
rs540969188 994 dbSNP
rs773452985 995 dbSNP
rs558491510 997 dbSNP
rs999472637 998 dbSNP
rs1287023722 1002 dbSNP
rs1331060987 1003 dbSNP
rs1453016752 1004 dbSNP
rs1363296390 1005 dbSNP
rs1299750576 1006 dbSNP
rs902642919 1006 dbSNP
rs1043792573 1007 dbSNP
rs1441635209 1017 dbSNP
rs1418139966 1021 dbSNP
rs1010956529 1022 dbSNP
rs573486266 1024 dbSNP
rs1174359252 1026 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084064. RNA binding protein: AGO2. Condition:CLIP_noemetine_AbnovaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084064
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_noemetine_AbnovaAb
Location of target site ENST00000264211.8 | 3UTR | CAAGGUAAGAAAGAUUUUUUUUUUUUU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
PRAD 0.549 0 0.395 0 44 Click to see details
UCEC 0.305 0.1 0.298 0.11 19 Click to see details
KIRC -0.136 0.14 -0.198 0.06 65 Click to see details
THCA 0.119 0.18 0.098 0.23 59 Click to see details
KICH -0.146 0.24 -0.190 0.18 25 Click to see details
PAAD 0.463 0.27 0.600 0.2 4 Click to see details
STAD 0.11 0.28 0.049 0.4 31 Click to see details
PCPG -0.604 0.29 -0.500 0.33 3 Click to see details
BLCA -0.124 0.32 -0.262 0.16 16 Click to see details
LUAD -0.171 0.33 0.150 0.35 9 Click to see details
CHOL -0.185 0.33 -0.167 0.35 8 Click to see details
HNSC 0.045 0.39 0.144 0.19 38 Click to see details
KIRP -0.043 0.41 -0.121 0.25 32 Click to see details
ESCA -0.097 0.42 0.000 0.5 7 Click to see details
CESC -0.244 0.42 -0.500 0.33 3 Click to see details
LUSC -0.033 0.42 -0.071 0.34 38 Click to see details
BRCA -0.021 0.43 -0.015 0.45 73 Click to see details
LIHC 0.006 0.48 0.097 0.26 45 Click to see details
LIHC 0.006 0.48 0.097 0.26 45 Click to see details
35 hsa-miR-3200-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT052815 CDC37 cell division cycle 37 1 1
MIRT052816 ATP6V1E2 ATPase H+ transporting V1 subunit E2 1 1
MIRT052817 MCM3AP minichromosome maintenance complex component 3 associated protein 1 1
MIRT052818 NSUN4 NOP2/Sun RNA methyltransferase family member 4 1 1
MIRT052819 EXOC4 exocyst complex component 4 1 1
MIRT052820 DDB1 damage specific DNA binding protein 1 1 1
MIRT052821 CAD carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase 1 1
MIRT052822 RPL23 ribosomal protein L23 1 1
MIRT052823 TPT1 tumor protein, translationally-controlled 1 1 1
MIRT052824 RPSA ribosomal protein SA 1 1
MIRT052825 PPIA peptidylprolyl isomerase A 1 1
MIRT052826 SSRP1 structure specific recognition protein 1 1 1
MIRT052827 STX2 syntaxin 2 1 1
MIRT095464 PURA purine rich element binding protein A 2 4
MIRT325192 PSAT1 phosphoserine aminotransferase 1 2 2
MIRT447293 ZNF562 zinc finger protein 562 2 2
MIRT450360 GRAMD3 GRAM domain containing 2B 2 2
MIRT482917 ZNF845 zinc finger protein 845 2 2
MIRT498302 DCAF8 DDB1 and CUL4 associated factor 8 2 2
MIRT500041 PABPC3 poly(A) binding protein cytoplasmic 3 2 8
MIRT541475 ARHGAP12 Rho GTPase activating protein 12 2 12
MIRT556269 MAPK6 mitogen-activated protein kinase 6 2 2
MIRT565440 SURF4 surfeit 4 2 2
MIRT573386 GPR20 G protein-coupled receptor 20 2 2
MIRT627911 OCIAD2 OCIA domain containing 2 2 2
MIRT633645 SKAP2 src kinase associated phosphoprotein 2 2 2
MIRT643472 PDK3 pyruvate dehydrogenase kinase 3 2 2
MIRT646284 GALNT2 polypeptide N-acetylgalactosaminyltransferase 2 2 2
MIRT647889 CD55 CD55 molecule (Cromer blood group) 2 2
MIRT664712 EIF4G3 eukaryotic translation initiation factor 4 gamma 3 2 2
MIRT668135 GINS2 GINS complex subunit 2 2 2
MIRT686490 TRIOBP TRIO and F-actin binding protein 2 2
MIRT709525 IGF2 insulin like growth factor 2 2 2
MIRT722252 NTMT1 N-terminal Xaa-Pro-Lys N-methyltransferase 1 2 2
MIRT755478 CAMK2A calcium/calmodulin dependent protein kinase II alpha 4 1
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-3200 Tamoxifen 2733525 NSC180973 approved resistant High Breast Cancer cell line (MCF-7, T47D)
hsa-mir-3200 Cisplatin 5460033 NSC119875 approved resistant High Gastric Cancer cell line (BGC823)
hsa-mir-3200 Ceritinib 57379345 NSC776422 approved sensitive High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-mir-3200 Tamoxifen 2733525 NSC180973 approved resistant cell line (MCF7)
hsa-mir-3200 Ceritinib 57379345 NSC776422 approved sensitive cell line (H3122)
hsa-mir-3200 Cisplatin 5460033 NSC119875 approved resistant cell line (BGC-823)
hsa-miR-3200-3p Fluorouracil 3385 NSC19893 approved resistant High Pancreatic Cancer cell line (PANC-1)
hsa-miR-3200-3p Gemcitabine 60750 NSC613327 approved resistant High Pancreatic Cancer cell line (PANC-1)
hsa-miR-3200-3p Plx-4720 24180719 NSC757438 sensitive High Thyroid Cancer cell line (8505c, BCPAP)
hsa-miR-3200-3p Ceritinib 57379345 NSC776422 approved sensitive High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-miR-3200-3p Vemurafenib 42611257 NSC761431 approved resistant cell line (451Lu)
hsa-miR-3200-3p Cisplatin 5460033 NSC119875 approved sensitive cell line
hsa-miR-3200-3p Gefitinib 123631 NSC715055 approved sensitive cell line (HCC827)
hsa-miR-3200-3p Paclitaxel 36314 NSC125973 approved sensitive cell line (HS578T)
hsa-miR-3200-3p Doxorubicin 31703 NSC123127 approved sensitive cell line (HS578T)
hsa-miR-3200-3p Fluorouracil 3385 NSC19893 approved sensitive cell line (HCT15)
hsa-miR-3200-3p Platinum 23939 resistant tissue (non-small cell lung cancer)
hsa-miR-3200-3p Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-3200-3p Ceritinib 57379345 NSC776422 approved sensitive cell line (H3122)
hsa-miR-3200-3p Paclitaxel/Docetaxel/Vinorelbine/Doxorubicin/Etoposide sensitive cell line (Bads-200)

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