pre-miRNA Information | |
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pre-miRNA | hsa-mir-103b-1 |
Genomic Coordinates | chr5: 168560904 - 168560965 |
Description | Homo sapiens miR-103b-1 stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Associated Diseases | ![]() |
pre-miRNA | hsa-mir-103b-2 |
Genomic Coordinates | chr20: 3917502 - 3917563 |
Description | Homo sapiens miR-103b-2 stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Associated Diseases | ![]() |
Mature miRNA Information | ||||||||||||||||||||||
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Mature miRNA | hsa-miR-103b | |||||||||||||||||||||
Sequence | 1| UCAUAGCCCUGUACAAUGCUGCU |23 | |||||||||||||||||||||
Evidence | Experimental | |||||||||||||||||||||
Experiments | ChIP-seq | DRVs in miRNA |
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SNPs in miRNA |
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Putative Targets |
Gene Information | |||||||||||||||||||||
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Gene Symbol | ELK1 | ||||||||||||||||||||
Synonyms | - | ||||||||||||||||||||
Description | ELK1, ETS transcription factor | ||||||||||||||||||||
Transcript | NM_001114123 | ||||||||||||||||||||
Other Transcripts | NM_005229 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on ELK1 | |||||||||||||||||||||
3'UTR of ELK1 (miRNA target sites are highlighted) |
>ELK1|NM_001114123|3'UTR 1 CTACTACCACCACCACCACCACCCCTTCTGGGGTCACTCCATCCATGCTCTCTCCAGCCAGCCATCTCAAGGAGAAACAT 81 AGTTCAACTGAAAGACTCATGCTCTGATTGTGGTGGGGTGGGGATCCTTGGGAAGAATTACTCCCAAGAGTAACTCTCAT 161 TATCTCCTCCACAGAAAACACACAGCTTCCACAACTTCTCTGTTTTCTGTCAGTCCCCCAGTGGCCGCCCTTACACGTCT 241 CCTACTTCAATGGTAGGGGCGGTTTATTTATTTATTTTTTGAAGGCCACTGGGAGGAGCCTGACCTAACCTTTTAGGGTG 321 GTTAGGACATCTCCCCCACCTCCCCACTTTTTTCCCCAAGACAAGACAATCGAGGTCTGGCTTGAGAACGACCTTTCTTT 401 CTTTATTTCTCAGCCTGCCCTTGGGGAGATGAGGGAGCCCTGTCTGCGTTTTTGGATGTGAGTAGAAGAGTTAGTTTGTT 481 TTGTTTTATTATTCCTGGCCATACTCAGGGGTCCAGGAAGAATTTGTACCATTTAATGGGTTGGGAGTCTTGGCCAAGGA 561 AGAATCACACCCTTGGAATAGAAATTTCCACCTCCCCAACCTTTCTCTCAGACAGCTTATCCTTTTCAACCAACTTTTTG 641 GCCAGGGAGGAATGTCCCTTTTGTTCTTCCCCCTGAGAAGCCATTCCTTTGTCTGCCAACCTCCCTGGGGTCCTGCCTGT 721 TTCCTCCCAATGGAGGGTTTTTTTGGGGGGTGGTCCCCGTCTGGGGGGCCCCTCCAGCCAGTACTCCAGGTCTCCCTGTC 801 TCTCCCCCGCTGCCATTTTGATAGTATAATCTATTTTTAAATGGGGCTTTTCAATAGGGGAGAGGGAGTCATCTCTTCCT 881 ATATTTGGTGGGGTGGGTGGGAAGGAAGGGATTTGGGGGGGAATCTTCCTGCCGCCTCCCCCACTCCAAGTGTTTATTTT 961 TGATACCAAACATGAATTTTCAGTTCCCTCCCTCCCAGCCCCCCAATTTCCTGCGGGCGGGTACAAAGGACCCTTTCAAT 1041 GTCCCTGGAGTTGGGAGGGAGGAATGGGGGACATAAAGCCTGTCCTGTCTCTATTCTAGGCAAGAGAGAGTGGGTTCAAA 1121 AGACTCCTGGGCTCACCTGTTAGCGCTGGCCCAGCCCAGGCCTTGGGACCTGGGGGTTGGTGATTTGGGGGACAGTGCTA 1201 CACTCGTCTCCACTGTTTGTTTTACTTCCCCAAAATGGACCTTTTTTTTTTCTAAAGAGTCCCAGAGAATGGGGAATTGT 1281 TCCTGTAAATATATATTTTTCAAAGTGATAAAAAAAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | HEK293S |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM1084064. RNA binding protein: AGO2. Condition:CLIP_noemetine_AbnovaAb
... - Karginov FV; Hannon GJ, 2013, Genes & development. |
Article |
- Karginov FV; Hannon GJ - Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
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CLIP-seq Support 1 for dataset GSM1084064 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_noemetine_AbnovaAb |
Location of target site | ENST00000247161.3 | 3UTR | AUCAGACCACUGCACUCCAGC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||||||||||||||||
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MiRNA-Target Expression Profile (TCGA) | |||||||
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53 hsa-miR-103b Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT218386 | E2F3 | E2F transcription factor 3 | ![]() |
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2 | 2 | ||||||
MIRT404221 | RPL7L1 | ribosomal protein L7 like 1 | ![]() |
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2 | 2 | ||||||
MIRT441502 | SPG20 | spartin | ![]() |
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2 | 6 | ||||||
MIRT444124 | ZNRF3 | zinc and ring finger 3 | ![]() |
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2 | 2 | ||||||
MIRT454236 | OSBPL10 | oxysterol binding protein like 10 | ![]() |
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2 | 4 | ||||||
MIRT457919 | ZNF212 | zinc finger protein 212 | ![]() |
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2 | 2 | ||||||
MIRT462254 | LAMA4 | laminin subunit alpha 4 | ![]() |
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2 | 2 | ||||||
MIRT463176 | ZNF281 | zinc finger protein 281 | ![]() |
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2 | 2 | ||||||
MIRT472355 | TSPAN1 | tetraspanin 1 | ![]() |
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2 | 2 | ||||||
MIRT474133 | LIN54 | lin-54 DREAM MuvB core complex component | ![]() |
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2 | 4 | ||||||
MIRT494946 | IFFO2 | intermediate filament family orphan 2 | ![]() |
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2 | 2 | ||||||
MIRT497403 | NPY4R | neuropeptide Y receptor Y4 | ![]() |
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2 | 2 | ||||||
MIRT497641 | GLDN | gliomedin | ![]() |
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2 | 2 | ||||||
MIRT505340 | TMEM245 | transmembrane protein 245 | ![]() |
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2 | 6 | ||||||
MIRT505680 | SESTD1 | SEC14 and spectrin domain containing 1 | ![]() |
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2 | 6 | ||||||
MIRT510706 | SREK1IP1 | SREK1 interacting protein 1 | ![]() |
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2 | 6 | ||||||
MIRT512198 | C1orf43 | chromosome 1 open reading frame 43 | ![]() |
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2 | 2 | ||||||
MIRT522089 | NUFIP2 | NUFIP2, FMR1 interacting protein 2 | ![]() |
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2 | 4 | ||||||
MIRT525074 | FRK | fyn related Src family tyrosine kinase | ![]() |
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2 | 2 | ||||||
MIRT531276 | PPIL3 | peptidylprolyl isomerase like 3 | ![]() |
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2 | 2 | ||||||
MIRT535119 | PLXNA2 | plexin A2 | ![]() |
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2 | 2 | ||||||
MIRT540836 | GNAT1 | G protein subunit alpha transducin 1 | ![]() |
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2 | 4 | ||||||
MIRT541018 | WIPI2 | WD repeat domain, phosphoinositide interacting 2 | ![]() |
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2 | 2 | ||||||
MIRT545752 | CA12 | carbonic anhydrase 12 | ![]() |
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2 | 4 | ||||||
MIRT546404 | SRP9 | signal recognition particle 9 | ![]() |
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2 | 2 | ||||||
MIRT547991 | HCFC2 | host cell factor C2 | ![]() |
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2 | 4 | ||||||
MIRT554502 | SAE1 | SUMO1 activating enzyme subunit 1 | ![]() |
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2 | 2 | ||||||
MIRT558360 | DMTF1 | cyclin D binding myb like transcription factor 1 | ![]() |
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2 | 2 | ||||||
MIRT558863 | CD2AP | CD2 associated protein | ![]() |
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2 | 2 | ||||||
MIRT559939 | ZNF567 | zinc finger protein 567 | ![]() |
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2 | 2 | ||||||
MIRT566300 | PPM1A | protein phosphatase, Mg2+/Mn2+ dependent 1A | ![]() |
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2 | 2 | ||||||
MIRT567490 | FOXK1 | forkhead box K1 | ![]() |
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2 | 2 | ||||||
MIRT617139 | ZNF556 | zinc finger protein 556 | ![]() |
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2 | 2 | ||||||
MIRT625400 | AKR7L | aldo-keto reductase family 7 like (gene/pseudogene) | ![]() |
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2 | 2 | ||||||
MIRT626491 | CEP89 | centrosomal protein 89 | ![]() |
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2 | 2 | ||||||
MIRT629487 | GSN | gelsolin | ![]() |
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2 | 4 | ||||||
MIRT638456 | PLXDC2 | plexin domain containing 2 | ![]() |
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2 | 2 | ||||||
MIRT648498 | CMBL | carboxymethylenebutenolidase homolog | ![]() |
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2 | 2 | ||||||
MIRT654845 | PPM1L | protein phosphatase, Mg2+/Mn2+ dependent 1L | ![]() |
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2 | 2 | ||||||
MIRT664587 | HSD17B12 | hydroxysteroid 17-beta dehydrogenase 12 | ![]() |
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2 | 2 | ||||||
MIRT664977 | TDRD1 | tudor domain containing 1 | ![]() |
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2 | 2 | ||||||
MIRT665028 | ELK1 | ELK1, ETS transcription factor | ![]() |
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2 | 2 | ||||||
MIRT666043 | STON2 | stonin 2 | ![]() |
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2 | 2 | ||||||
MIRT668863 | CRY2 | cryptochrome circadian clock 2 | ![]() |
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2 | 2 | ||||||
MIRT669297 | C17orf85 | nuclear cap binding subunit 3 | ![]() |
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2 | 2 | ||||||
MIRT680975 | DCAF17 | DDB1 and CUL4 associated factor 17 | ![]() |
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2 | 2 | ||||||
MIRT682267 | RS1 | retinoschisin 1 | ![]() |
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2 | 2 | ||||||
MIRT685283 | KIAA1143 | KIAA1143 | ![]() |
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2 | 2 | ||||||
MIRT693375 | PIGP | phosphatidylinositol glycan anchor biosynthesis class P | ![]() |
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2 | 2 | ||||||
MIRT701785 | MSL1 | male specific lethal 1 homolog | ![]() |
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2 | 2 | ||||||
MIRT709373 | SPECC1 | sperm antigen with calponin homology and coiled-coil domains 1 | ![]() |
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2 | 2 | ||||||
MIRT715154 | IL12B | interleukin 12B | ![]() |
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2 | 2 | ||||||
MIRT734499 | ADAMTS5 | ADAM metallopeptidase with thrombospondin type 1 motif 5 | ![]() |
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3 | 0 |
miRNA-Drug Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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