>ARPC2|NM_005731|3'UTR
   1 TCTTGGGAATAAGAGGAGGAAGCGGCTGGCAACTGAAGGCTGGAACACTTGCTACTGGATAATCGTAGCTTTTAATGTTG
  81 CGCCTCTTCAGGTTCTTAAGGGATTCTCCGTTTTGGTTCCATTTTGTACACGTTTGGAAAATAATCTGCAGAAACGAGCT
 161 GTGCTTGCAAAGACTTCATAGTTCCCAAGAATTAAAAAAAAAAAAAAAAGAATTCCACTTGATCAACTTAATTCCTTTTC
 241 TTTATCTTCCCTCCCTCACTTCCCTTTTCTCCCACCCTCTTTTCCAAGCTGTTTCGCTTTGCAATATATTACTGGTAATG
 321 AGTTGCAGGATAATGCAGTCATAACTTGTTTTCTCCTAAGTATTTGAGTTCAAAACTCCTGTATCTAAAGAAATACGGTT
 401 GGGGTCATTAATAAAGAAAATCTTTCTATCTTAAAAAAAAAA

ID	Duplex structure	Position	Score	MFE
1	miRNA 3' ucUCCGUCC-----AA-G--GACCUAGUa 5' |||| || || | |||||| | Target 5' gaAGGCTGGAACACTTGCTACTGGATAAt 3'	35 - 63	121.00	-15.42
2	miRNA 3' ucuccguccAAG--GACCUAGUa 5' ||| || ||||| Target 5' aaaaaagaaTTCCACTTGATCAa 3'	204 - 226	118.00	-5.60
3	miRNA 3' ucuccguCCAAGGACCUAGUa 5' |||| ||:||| Target 5' gaaatacGGTTG--GGGTCAt 3'	390 - 408	105.00	-11.90

Mutant ID	Mutant Position	Mutant Source
COSN30107346	23	COSMIC
COSN30118443	24	COSMIC
COSN17132134	25	COSMIC
COSN31564272	65	COSMIC
COSN30495708	83	COSMIC
COSN30156398	87	COSMIC
COSN13970839	109	COSMIC
COSN6169600	142	COSMIC
COSN24299556	156	COSMIC
COSN23015929	190	COSMIC
COSN20097826	210	COSMIC
COSN28962358	210	COSMIC
COSN21171738	319	COSMIC

Mutant ID	Mutant Position	Mutant Source
rs969608629	2	dbSNP
rs775251880	6	dbSNP
rs763139971	11	dbSNP
rs762741833	14	dbSNP
rs201131392	16	dbSNP
rs979589589	17	dbSNP
rs1477391180	19	dbSNP
rs903563573	23	dbSNP
rs773762403	24	dbSNP
rs368620700	25	dbSNP
rs1329258475	30	dbSNP
rs1803063	37	dbSNP
rs1803066	40	dbSNP
rs767919551	44	dbSNP
rs750992729	47	dbSNP
rs1211332036	48	dbSNP
rs1232680027	53	dbSNP
rs756470622	56	dbSNP
rs935084825	60	dbSNP
rs766773047	64	dbSNP
rs149956309	65	dbSNP
rs779013233	66	dbSNP
rs1198565391	67	dbSNP
rs748183080	81	dbSNP
rs757161892	82	dbSNP
rs780978675	83	dbSNP
rs745840386	86	dbSNP
rs756347491	90	dbSNP
rs1186527712	91	dbSNP
rs1427364041	93	dbSNP
rs1309523674	94	dbSNP
rs771213264	98	dbSNP
rs769688778	102	dbSNP
rs775446564	103	dbSNP
rs1370290480	105	dbSNP
rs1220322542	106	dbSNP
rs182444966	107	dbSNP
rs768195488	109	dbSNP
rs773850659	110	dbSNP
rs562800634	111	dbSNP
rs943928958	122	dbSNP
rs201998304	127	dbSNP
rs1028884998	133	dbSNP
rs899644688	156	dbSNP
rs1425749305	157	dbSNP
rs1192774442	167	dbSNP
rs73086112	181	dbSNP
rs1299046752	187	dbSNP
rs1178818595	188	dbSNP
rs879157920	190	dbSNP
rs1197420721	192	dbSNP
rs1226726155	193	dbSNP
rs1281330742	194	dbSNP
rs376913304	194	dbSNP
rs879085335	194	dbSNP
rs1272762218	195	dbSNP
rs1321183077	211	dbSNP
rs1359734148	218	dbSNP
rs760743331	228	dbSNP
rs569406671	232	dbSNP
rs972124286	245	dbSNP
rs1036211808	246	dbSNP
rs960213259	264	dbSNP
rs887833022	273	dbSNP
rs1235970680	275	dbSNP
rs992070526	291	dbSNP
rs1004971490	296	dbSNP
rs1014907293	297	dbSNP
rs545341211	305	dbSNP
rs979024952	307	dbSNP
rs560728818	315	dbSNP
rs1023934766	319	dbSNP
rs1249881334	332	dbSNP
rs1308789536	338	dbSNP
rs1453493841	346	dbSNP
rs1381350173	349	dbSNP
rs969953449	356	dbSNP
rs979591865	357	dbSNP
rs1304533197	359	dbSNP
rs1445791620	361	dbSNP
rs1032926341	370	dbSNP
rs1298649516	370	dbSNP
rs956459357	373	dbSNP
rs1421273072	381	dbSNP
rs1378111434	384	dbSNP
rs1157605559	386	dbSNP
rs987862776	388	dbSNP
rs1052163959	395	dbSNP
rs531820099	397	dbSNP
rs965037465	405	dbSNP
rs975298090	406	dbSNP
rs933127586	413	dbSNP
rs1415207295	421	dbSNP
rs764194359	429	dbSNP

- Genes & development, 2013

When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.