pre-miRNA Information
pre-miRNA hsa-mir-4486   
Genomic Coordinates chr11: 19575310 - 19575372
Description Homo sapiens miR-4486 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4486
Sequence 5| GCUGGGCGAGGCUGGCA |21
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs774299284 8 dbSNP
rs902172817 14 dbSNP
rs1222795726 17 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol KIAA1551   
Synonyms C12orf35, GET, UTA2-1
Description KIAA1551
Transcript NM_018169   
Expression
Putative miRNA Targets on KIAA1551
3'UTR of KIAA1551
(miRNA target sites are highlighted)
>KIAA1551|NM_018169|3'UTR
   1 TTACAAGATGTGGTTTTGTAATTGCCACTGGGAAATTTCTTTCCTTTTCTGTTCAAAATATTTCGCTGAAACTAATGAGA
  81 AATGCCATGATAAAGATTTCTCAGAGTTTGGTTCCCACTTTCATTGTATTTCATTGAAAGTGCTTAATTAAAATGGCTTG
 161 AGAACTTTGGGTAGCCATGTGTAAGAAATGGATGGTATTCACCGGGGAAACAAGGTATTTGAATTTCTACTTTATTGAAC
 241 CAGATTTACCATTATTTTAAAAGGAATGCTTATACAAATCAATTTGAAATTCTACCCATCTTGAGGGAGGACCGTTCCTC
 321 AGTTAAGGACTTGTTTATTTAAATGGGACTGTAAATATGTTTTGGTTTCTAAGCTATATTAGCAAAATTTATTTTTCAAA
 401 AATGCCCACTGTGATGTGAATGTCAAAATATATTCTTAAGTGTTTTATAACTAATTGTAAACTTTTTTTCAGAAGTCTTA
 481 TTTTATACTTGTGAAACTGAACACAATTTTGGGACAACGTTTAAACATTACTTTTCATACTTGAAATAAACATTTATTTT
 561 TTAAAAAACTAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' acggucggaGCGGGUcg 5'
                   :|||||  
Target 5' tttcaaaaaTGCCCAct 3'
394 - 410 104.00 -8.10
2
miRNA  3' acggUCGGAGCGGGUCg 5'
              | | |: |:||| 
Target 5' gaggACCGTTCCTCAGt 3'
307 - 323 85.00 -10.90
3
miRNA  3' acggucggagcGGGUCg 5'
                     |:||| 
Target 5' gataaagatttCTCAGa 3'
89 - 105 84.00 -6.00
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30511422 8 COSMIC
COSN30511420 9 COSMIC
COSN19462192 31 COSMIC
COSN30453398 44 COSMIC
COSN20077742 52 COSMIC
COSN30155073 55 COSMIC
COSN31581171 63 COSMIC
COSN30446987 65 COSMIC
COSN30148604 88 COSMIC
COSN30152275 96 COSMIC
COSN31525488 99 COSMIC
COSN31586002 101 COSMIC
COSN8586000 124 COSMIC
COSN28637255 170 COSMIC
COSN22536184 189 COSMIC
COSN31566841 301 COSMIC
COSN29869481 448 COSMIC
COSN31486611 544 COSMIC
COSN30372258 561 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1244471639 1 dbSNP
rs140283743 2 dbSNP
rs761558428 2 dbSNP
rs769633524 3 dbSNP
rs771759935 4 dbSNP
rs1057085580 5 dbSNP
rs915794016 6 dbSNP
rs369511087 17 dbSNP
rs1388735950 19 dbSNP
rs772819540 21 dbSNP
rs1214383707 33 dbSNP
rs760279522 36 dbSNP
rs1201953055 39 dbSNP
rs771305776 40 dbSNP
rs1262788038 42 dbSNP
rs1184206376 44 dbSNP
rs776800275 47 dbSNP
rs1465553653 49 dbSNP
rs1469897400 52 dbSNP
rs184472712 57 dbSNP
rs11539999 60 dbSNP
rs948440131 61 dbSNP
rs1410938562 62 dbSNP
rs1176610361 65 dbSNP
rs574353842 66 dbSNP
rs1038271779 76 dbSNP
rs552563704 77 dbSNP
rs1464927060 86 dbSNP
rs1249355630 89 dbSNP
rs1207323884 90 dbSNP
rs1464893413 92 dbSNP
rs117220556 93 dbSNP
rs190222249 94 dbSNP
rs146822999 104 dbSNP
rs750910356 113 dbSNP
rs114323263 116 dbSNP
rs562183763 122 dbSNP
rs943946302 123 dbSNP
rs1318690280 125 dbSNP
rs1039603617 136 dbSNP
rs529792454 149 dbSNP
rs1459665882 154 dbSNP
rs549929126 160 dbSNP
rs899746214 169 dbSNP
rs541410860 172 dbSNP
rs998743266 176 dbSNP
rs563258674 178 dbSNP
rs1467349557 185 dbSNP
rs79108287 188 dbSNP
rs1021865622 189 dbSNP
rs1174840475 191 dbSNP
rs1449715882 194 dbSNP
rs1400976670 197 dbSNP
rs375774394 204 dbSNP
rs998987219 205 dbSNP
rs553660884 225 dbSNP
rs1202736670 226 dbSNP
rs1482442556 230 dbSNP
rs139268880 234 dbSNP
rs1206119686 244 dbSNP
rs758923720 245 dbSNP
rs1344668189 247 dbSNP
rs1295458289 255 dbSNP
rs959665687 256 dbSNP
rs991703439 260 dbSNP
rs1022680670 264 dbSNP
rs1228202883 267 dbSNP
rs1311428484 268 dbSNP
rs1313300369 270 dbSNP
rs747326881 274 dbSNP
rs77653313 281 dbSNP
rs973624841 287 dbSNP
rs1312115495 297 dbSNP
rs966794374 300 dbSNP
rs978208496 301 dbSNP
rs1283427136 304 dbSNP
rs1487879924 307 dbSNP
rs769119076 310 dbSNP
rs1190149977 313 dbSNP
rs542069976 314 dbSNP
rs181364628 315 dbSNP
rs993055421 334 dbSNP
rs752285759 345 dbSNP
rs911901155 348 dbSNP
rs1204749541 350 dbSNP
rs1431478138 351 dbSNP
rs943391649 359 dbSNP
rs948620564 360 dbSNP
rs1039048411 375 dbSNP
rs563660359 377 dbSNP
rs899777644 389 dbSNP
rs1338667485 390 dbSNP
rs947274834 398 dbSNP
rs1042913432 410 dbSNP
rs902987734 416 dbSNP
rs1295 419 dbSNP
rs1446457655 421 dbSNP
rs1157147502 431 dbSNP
rs746970857 433 dbSNP
rs1164560859 436 dbSNP
rs1035362259 437 dbSNP
rs895566343 447 dbSNP
rs1417838276 454 dbSNP
rs1012568442 457 dbSNP
rs1181663674 463 dbSNP
rs1250076895 463 dbSNP
rs1473754001 463 dbSNP
rs900918024 466 dbSNP
rs1269557365 474 dbSNP
rs1208185765 479 dbSNP
rs934189358 486 dbSNP
rs1050327668 490 dbSNP
rs1228932359 492 dbSNP
rs1420738503 498 dbSNP
rs1330875791 499 dbSNP
rs1023039036 506 dbSNP
rs1300412835 507 dbSNP
rs890048016 514 dbSNP
rs1324932062 515 dbSNP
rs1384519167 516 dbSNP
rs780321126 517 dbSNP
rs537135102 519 dbSNP
rs902552656 520 dbSNP
rs1410199616 527 dbSNP
rs564923172 528 dbSNP
rs1478464748 529 dbSNP
rs777586179 539 dbSNP
rs1379536927 540 dbSNP
rs1197058812 557 dbSNP
rs1451038233 563 dbSNP
rs1035131023 564 dbSNP
rs1026492865 569 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084047. RNA binding protein: AGO2. Condition:CLIP_arsenite_rep4 ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' acGGUCGGAGCGGGUCg 5'
            ||| || ||||||| 
Target 5' cgCCA-CCACGCCCAG- 3'
22 - 36
2
miRNA  3' acggucggagcggGUCg 5'
                       ||| 
Target 5' guagcugggauuaCAGg 3'
1 - 17
Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084047
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_arsenite_rep4
Location of target site ENST00000312561.4 | 3UTR | GUAGCUGGGAUUACAGGCGCCCGCCACCACGCCCAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
108 hsa-miR-4486 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT254654 NF2 neurofibromin 2 2 2
MIRT458684 MRI1 methylthioribose-1-phosphate isomerase 1 2 2
MIRT470845 PLXND1 plexin D1 2 2
MIRT493011 NANOS1 nanos C2HC-type zinc finger 1 2 2
MIRT497264 GRK6 G protein-coupled receptor kinase 6 2 2
MIRT497675 SYNGR1 synaptogyrin 1 2 2
MIRT498219 TLN2 talin 2 2 2
MIRT498310 BCL11B B-cell CLL/lymphoma 11B 2 2
MIRT504048 TOMM5 translocase of outer mitochondrial membrane 5 2 2
MIRT519959 ZCCHC8 zinc finger CCHC-type containing 8 2 2
MIRT531521 NOM1 nucleolar protein with MIF4G domain 1 2 2
MIRT533144 WNT10A Wnt family member 10A 2 2
MIRT533541 TPR translocated promoter region, nuclear basket protein 2 2
MIRT533681 TMEM86A transmembrane protein 86A 2 2
MIRT540321 PIGR polymeric immunoglobulin receptor 2 2
MIRT540719 GUF1 GUF1 homolog, GTPase 2 2
MIRT541566 ZNF43 zinc finger protein 43 2 4
MIRT541787 TBCCD1 TBCC domain containing 1 2 2
MIRT541925 ORC1 origin recognition complex subunit 1 2 4
MIRT542232 FUT9 fucosyltransferase 9 2 2
MIRT542285 POLR3K RNA polymerase III subunit K 2 2
MIRT542299 QTRTD1 queuine tRNA-ribosyltransferase accessory subunit 2 2 4
MIRT542368 PAICS phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthase 2 2
MIRT542441 C3 complement C3 2 4
MIRT542475 APOC3 apolipoprotein C3 2 2
MIRT542535 MRPS10 mitochondrial ribosomal protein S10 2 2
MIRT542640 TIMM8A translocase of inner mitochondrial membrane 8A 2 2
MIRT542788 PLEKHA3 pleckstrin homology domain containing A3 2 2
MIRT552104 PPP1R1A protein phosphatase 1 regulatory inhibitor subunit 1A 2 2
MIRT564913 YTHDF1 YTH N6-methyladenosine RNA binding protein 1 2 2
MIRT568606 ACVR2A activin A receptor type 2A 2 2
MIRT607389 LANCL3 LanC like 3 2 2
MIRT607451 ZNF543 zinc finger protein 543 2 2
MIRT610058 MYBPC1 myosin binding protein C, slow type 2 2
MIRT610793 KLK2 kallikrein related peptidase 2 2 2
MIRT617176 GOSR2 golgi SNAP receptor complex member 2 2 2
MIRT620579 WBSCR27 methyltransferase like 27 2 4
MIRT622085 SRPX2 sushi repeat containing protein, X-linked 2 2 2
MIRT622542 PXMP4 peroxisomal membrane protein 4 2 2
MIRT630009 PDE6B phosphodiesterase 6B 2 2
MIRT631813 PTDSS2 phosphatidylserine synthase 2 2 2
MIRT632721 MSANTD4 Myb/SANT DNA binding domain containing 4 with coiled-coils 2 2
MIRT632757 MED28 mediator complex subunit 28 2 2
MIRT634821 ASB6 ankyrin repeat and SOCS box containing 6 2 2
MIRT635255 FBXL20 F-box and leucine rich repeat protein 20 2 2
MIRT637082 SELPLG selectin P ligand 2 2
MIRT637357 ZNF460 zinc finger protein 460 2 2
MIRT637472 DEFB105B defensin beta 105B 2 4
MIRT637504 DEFB105A defensin beta 105A 2 4
MIRT639022 AAK1 AP2 associated kinase 1 2 2
MIRT641012 ANKFY1 ankyrin repeat and FYVE domain containing 1 2 2
MIRT642170 HEBP2 heme binding protein 2 2 2
MIRT648977 ACAD8 acyl-CoA dehydrogenase family member 8 2 2
MIRT650515 UFM1 ubiquitin fold modifier 1 2 2
MIRT650949 INMT indolethylamine N-methyltransferase 2 2
MIRT658354 FAM65B RHO family interacting cell polarization regulator 2 2 2
MIRT660736 ALG14 ALG14, UDP-N-acetylglucosaminyltransferase subunit 2 2
MIRT662191 MEI1 meiotic double-stranded break formation protein 1 2 2
MIRT663045 SLC16A4 solute carrier family 16 member 4 2 2
MIRT664811 IRAK3 interleukin 1 receptor associated kinase 3 2 2
MIRT665161 SF3A1 splicing factor 3a subunit 1 2 4
MIRT665346 YES1 YES proto-oncogene 1, Src family tyrosine kinase 2 2
MIRT666493 SBNO1 strawberry notch homolog 1 2 2
MIRT666545 RNF115 ring finger protein 115 2 2
MIRT669351 BMP3 bone morphogenetic protein 3 2 2
MIRT669904 KIAA0754 KIAA0754 2 4
MIRT670323 CEP57L1 centrosomal protein 57 like 1 2 2
MIRT670430 ELP2 elongator acetyltransferase complex subunit 2 2 2
MIRT670672 KIAA1551 KIAA1551 2 2
MIRT670746 HOOK3 hook microtubule tethering protein 3 2 2
MIRT670998 PTGIS prostaglandin I2 synthase 2 2
MIRT671290 RPL37A ribosomal protein L37a 2 2
MIRT671469 AGPAT6 glycerol-3-phosphate acyltransferase 4 2 2
MIRT671833 STIL STIL, centriolar assembly protein 2 2
MIRT673006 TAF1 TATA-box binding protein associated factor 1 2 2
MIRT675881 CSTF1 cleavage stimulation factor subunit 1 2 2
MIRT678625 OLFML2A olfactomedin like 2A 2 2
MIRT678790 NUPL2 nucleoporin like 2 2 2
MIRT679560 LIN9 lin-9 DREAM MuvB core complex component 2 2
MIRT681032 AAED1 AhpC/TSA antioxidant enzyme domain containing 1 2 2
MIRT682480 LIX1L limb and CNS expressed 1 like 2 2
MIRT682758 MDM2 MDM2 proto-oncogene 2 2
MIRT682810 TMCO1 transmembrane and coiled-coil domains 1 2 2
MIRT682867 C9orf156 tRNA methyltransferase O 2 2
MIRT689233 RPS19 ribosomal protein S19 2 2
MIRT689305 C5AR2 complement component 5a receptor 2 2 2
MIRT689363 ZNF101 zinc finger protein 101 2 2
MIRT689629 NAA30 N(alpha)-acetyltransferase 30, NatC catalytic subunit 2 2
MIRT689654 RBM23 RNA binding motif protein 23 2 2
MIRT690148 PPIL6 peptidylprolyl isomerase like 6 2 2
MIRT691407 DNA2 DNA replication helicase/nuclease 2 2 2
MIRT691847 OSCAR osteoclast associated, immunoglobulin-like receptor 2 2
MIRT692234 ALDH1B1 aldehyde dehydrogenase 1 family member B1 2 2
MIRT694320 NLRP9 NLR family pyrin domain containing 9 2 2
MIRT694365 CHST6 carbohydrate sulfotransferase 6 2 2
MIRT696215 LYZ lysozyme 2 2
MIRT697230 ZYG11A zyg-11 family member A, cell cycle regulator 2 2
MIRT700487 PTPRF protein tyrosine phosphatase, receptor type F 2 2
MIRT700612 PRKCA protein kinase C alpha 2 2
MIRT702456 KIAA1467 family with sequence similarity 234 member B 2 2
MIRT702990 HERPUD2 HERPUD family member 2 2 2
MIRT703998 EIF5A2 eukaryotic translation initiation factor 5A2 2 2
MIRT704701 CHRFAM7A CHRNA7 (exons 5-10) and FAM7A (exons A-E) fusion 2 2
MIRT712481 FSTL3 follistatin like 3 2 2
MIRT712781 ZNF154 zinc finger protein 154 2 2
MIRT714369 HP1BP3 heterochromatin protein 1 binding protein 3 2 2
MIRT722570 C1orf95 stum, mechanosensory transduction mediator homolog 2 2
MIRT722839 C17orf102 chromosome 17 open reading frame 102 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-4 Dexamethasone approved 5743 Microarray primary rat thymocytes 20847043 2010 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-4486 Dabrafenib 44462760 NSC764134 approved resistant High Melanoma cell line (A375, IGR37, 501Mel)
hsa-miR-4486 Vemurafenib 42611257 NSC761431 approved resistant High Melanoma cell line (A375, IGR37, 501Mel)
hsa-miR-4486 Doxorubicin 31703 NSC123127 approved sensitive High Breast Cancer cell line (MCF-7)
hsa-miR-4486 Curcumin 969516 NSC32982 approved sensitive High Breast Cancer cell line (MCF-7)
hsa-miR-4486 Paclitaxel 36314 NSC125973 approved resistant High Non-Small Cell Lung Cancer cell line (A549)
hsa-miR-4486 Fulvestrant 17756771 NSC719276 approved sensitive High Breast Cancer cell line (MCF-7)
hsa-miR-4486 Tamoxifen 2733525 NSC180973 approved resistant High Breast Cancer cell line (MCF-7)
hsa-miR-4486 Cisplatin 5460033 NSC119875 approved sensitive High Gastric Cancer cell line (SGC7901)
hsa-miR-4486 Cisplatin 5460033 NSC119875 approved sensitive Low Gastric Cancer cell line (SGC-7901)
hsa-mir-4486 Dabrafenib 44462760 NSC764134 approved resistant cell line (A375)
hsa-mir-4486 Cisplatin 5460033 NSC119875 approved resistant cell line (BxPC3)
hsa-miR-4486 Temozolomide 5394 NSC362856 approved resistant cell line (U251)
hsa-miR-4486 Doxorubicin 31703 NSC123127 approved resistant cell line (BAS)
hsa-miR-4486 Doxorubicin 31703 NSC123127 approved resistant cell line (HS578T)
hsa-miR-4486 Cisplatin 5460033 NSC119875 approved resistant cell line (A2780)
hsa-miR-4486 Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (1500 ng/ml)
hsa-miR-4486 Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (100 ng/ml)
hsa-miR-4486 Gemcitabine 60750 NSC613327 approved resistant cell line (Panc1-GR4)

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