ID	Duplex structure	Position	Score	MFE
1	miRNA 3' ugGUUUCCGUCGG--GACCGUUGa 5' || ||| | |: ||| |||| Target 5' ctCACAGG-ACCTGGCTGTCAACc 3'	23 - 45	125.00	-14.91
2	miRNA 3' ugguUUCCGUCGGGACCGUUga 5' :|| :||::|| ||| Target 5' ----GAGTGGGCTTTGACAAac 3'	1 - 18	106.00	-8.00
3	miRNA 3' ugGUUUCCGUCGGGACCGUUga 5' |||| | :|:||| || Target 5' caCAAA--C-TTCTTGGAAAga 3'	96 - 114	99.00	-6.30

Mutant ID	Mutant Position	Mutant Source
COSN30479841	2	COSMIC
COSN28198026	3	COSMIC
COSN31561345	26	COSMIC
COSN30493224	37	COSMIC
COSN30493158	74	COSMIC
COSN22784388	172	COSMIC
COSN32062054	369	COSMIC
COSN23205694	370	COSMIC
COSN6456826	392	COSMIC
COSN7220248	911	COSMIC
COSN22117303	1342	COSMIC
COSN15991761	2418	COSMIC
COSN25584201	3335	COSMIC
COSN27190222	3348	COSMIC

Mutant ID	Mutant Position	Mutant Source
rs1467690579	9	dbSNP
rs1401155252	15	dbSNP
rs755691908	17	dbSNP
rs1342980928	19	dbSNP
rs1454094906	20	dbSNP
rs777181057	21	dbSNP
rs1381532844	22	dbSNP
rs1450939669	26	dbSNP
rs1281577362	27	dbSNP
rs199769153	28	dbSNP
rs576024869	31	dbSNP
rs773926708	32	dbSNP
rs767610290	34	dbSNP
rs1204343799	35	dbSNP
rs371711865	40	dbSNP
rs768888249	44	dbSNP
rs1460106320	45	dbSNP
rs777243182	46	dbSNP
rs1476305384	47	dbSNP
rs115640157	51	dbSNP
rs372333627	52	dbSNP
rs1160280635	55	dbSNP
rs953696043	68	dbSNP
rs985174669	72	dbSNP
rs1358546674	75	dbSNP
rs1211957746	88	dbSNP
rs1032755238	90	dbSNP
rs1216562228	94	dbSNP
rs558746967	100	dbSNP
rs957148167	101	dbSNP
rs888023280	107	dbSNP
rs1373887145	110	dbSNP
rs1327288432	112	dbSNP
rs988646883	114	dbSNP
rs572375268	116	dbSNP
rs780780157	123	dbSNP
rs901614219	124	dbSNP
rs1224682340	128	dbSNP
rs971315321	134	dbSNP
rs997197058	148	dbSNP
rs1167756893	161	dbSNP
rs1454642317	164	dbSNP
rs541332334	174	dbSNP
rs1191826158	180	dbSNP
rs1468140152	180	dbSNP
rs1233527272	195	dbSNP
rs927261748	196	dbSNP
rs937333420	197	dbSNP
rs1272713660	214	dbSNP
rs192464263	223	dbSNP
rs956795261	224	dbSNP
rs1301953783	228	dbSNP
rs1444156598	229	dbSNP
rs909280762	230	dbSNP
rs184583257	232	dbSNP
rs1036505070	234	dbSNP
rs902029555	236	dbSNP
rs41269519	245	dbSNP
rs1050674641	253	dbSNP
rs1180266059	260	dbSNP
rs1352451675	261	dbSNP
rs191791336	269	dbSNP
rs981547953	273	dbSNP
rs889405809	274	dbSNP
rs531652538	289	dbSNP
rs1377183579	292	dbSNP
rs1171608020	302	dbSNP
rs1006560080	304	dbSNP
rs1408746407	311	dbSNP
rs985155592	313	dbSNP
rs1468765563	318	dbSNP
rs575564390	321	dbSNP
rs1486823567	325	dbSNP
rs1278643181	329	dbSNP
rs1210749314	335	dbSNP
rs1318075533	336	dbSNP
rs1032703093	338	dbSNP
rs957114416	339	dbSNP
rs6670611	340	dbSNP
rs749275557	344	dbSNP
rs1415035658	345	dbSNP
rs1357083882	349	dbSNP
rs1333002304	355	dbSNP
rs1036506938	366	dbSNP
rs200171510	366	dbSNP
rs1161368140	368	dbSNP
rs1335968863	368	dbSNP
rs1185222650	369	dbSNP
rs1246855492	369	dbSNP
rs1349254275	369	dbSNP
rs1399083418	369	dbSNP
rs1422114513	369	dbSNP
rs35859868	369	dbSNP
rs61269457	369	dbSNP
rs1020076710	371	dbSNP
rs1306635965	373	dbSNP
rs971642919	373	dbSNP
rs1375095435	375	dbSNP
rs1239908010	377	dbSNP
rs1257076307	380	dbSNP
rs1424533561	383	dbSNP
rs981329042	383	dbSNP
rs1347803735	387	dbSNP
rs1198799942	389	dbSNP
rs927208133	389	dbSNP
rs11210714	392	dbSNP
rs749472119	392	dbSNP
rs984744763	398	dbSNP
rs1438482557	405	dbSNP
rs1208827843	406	dbSNP
rs1180697595	411	dbSNP
rs1252834901	412	dbSNP
rs1202816395	422	dbSNP
rs1341370801	425	dbSNP
rs1304146563	426	dbSNP
rs796760991	432	dbSNP
rs1236424172	434	dbSNP
rs1236602090	437	dbSNP
rs933474744	448	dbSNP
rs1050166254	456	dbSNP
rs1194068072	462	dbSNP
rs888858687	468	dbSNP
rs1398957335	471	dbSNP
rs538529800	473	dbSNP
rs755464931	476	dbSNP
rs1171940331	478	dbSNP
rs1175999798	484	dbSNP
rs1396509541	485	dbSNP
rs1466761611	488	dbSNP
rs1328811809	490	dbSNP
rs1419274469	492	dbSNP
rs528017772	495	dbSNP
rs112941067	496	dbSNP
rs1482573397	499	dbSNP
rs1302496926	500	dbSNP
rs1318065750	500	dbSNP
rs892398146	500	dbSNP
rs1009466723	503	dbSNP
rs1255456204	504	dbSNP
rs1333323572	506	dbSNP
rs12565942	508	dbSNP
rs1286618052	512	dbSNP
rs1302004197	512	dbSNP
rs1315209957	513	dbSNP
rs116651425	515	dbSNP
rs1365045533	515	dbSNP
rs1453925239	515	dbSNP
rs772291807	515	dbSNP
rs143742810	517	dbSNP
rs1472079133	517	dbSNP
rs75117028	518	dbSNP
rs1318635244	520	dbSNP
rs577392466	523	dbSNP
rs1205660535	526	dbSNP
rs58052136	526	dbSNP
rs1256360680	527	dbSNP
rs1352839054	527	dbSNP
rs202065706	528	dbSNP
rs1034440700	532	dbSNP
rs959007992	541	dbSNP
rs984821495	545	dbSNP
rs1449658518	550	dbSNP
rs933477195	552	dbSNP
rs1319497923	553	dbSNP
rs1198034226	562	dbSNP
rs1373596599	568	dbSNP
rs146120444	583	dbSNP
rs889353512	586	dbSNP
rs1001130019	587	dbSNP
rs1054098548	590	dbSNP
rs1166809270	604	dbSNP
rs892793954	606	dbSNP
rs1471654903	611	dbSNP
rs1422469121	615	dbSNP
rs1367677960	619	dbSNP
rs923515876	621	dbSNP
rs1461696052	629	dbSNP
rs1294942606	634	dbSNP
rs1246238778	643	dbSNP
rs1208234633	647	dbSNP
rs984269231	648	dbSNP
rs1009951298	657	dbSNP
rs1020445943	659	dbSNP
rs1226839778	681	dbSNP
rs1393249204	683	dbSNP
rs1271171824	686	dbSNP
rs1385471100	688	dbSNP
rs1323447109	702	dbSNP
rs1363711611	711	dbSNP
rs1341720807	712	dbSNP
rs1433785930	717	dbSNP
rs1220535793	720	dbSNP
rs933528557	720	dbSNP
rs1050517249	725	dbSNP
rs756985588	727	dbSNP
rs910311330	743	dbSNP
rs549475577	748	dbSNP
rs971209160	760	dbSNP
rs1002642382	762	dbSNP
rs1245710432	774	dbSNP
rs1267488888	778	dbSNP
rs1034173669	779	dbSNP
rs1487355488	790	dbSNP
rs569744811	793	dbSNP
rs1454724158	807	dbSNP
rs1254969604	815	dbSNP
rs958587397	815	dbSNP
rs1219603135	816	dbSNP
rs1451278198	821	dbSNP
rs1274128010	825	dbSNP
rs984692309	826	dbSNP
rs140081553	828	dbSNP
rs1340638856	842	dbSNP
rs1016227329	848	dbSNP
rs558838896	851	dbSNP
rs892150050	852	dbSNP
rs189803995	858	dbSNP
rs1276128863	859	dbSNP
rs1433824544	861	dbSNP
rs1330190370	862	dbSNP
rs868630700	864	dbSNP
rs1329295015	871	dbSNP
rs1431462543	873	dbSNP
rs59119417	874	dbSNP
rs1167813104	883	dbSNP
rs780733330	889	dbSNP
rs1190864710	906	dbSNP
rs1261768592	922	dbSNP
rs1475254086	923	dbSNP
rs554826564	923	dbSNP
rs1166405306	924	dbSNP
rs933444891	924	dbSNP
rs1396919266	927	dbSNP
rs986287061	928	dbSNP
rs1477805899	937	dbSNP
rs906447729	947	dbSNP
rs910740774	950	dbSNP
rs745481077	951	dbSNP
rs142300553	958	dbSNP
rs769720150	960	dbSNP
rs1482237825	962	dbSNP
rs775371124	969	dbSNP
rs749300396	971	dbSNP
rs1350740562	974	dbSNP
rs1033900267	981	dbSNP
rs892749831	986	dbSNP
rs768442952	989	dbSNP
rs1041405684	991	dbSNP
rs1163106068	996	dbSNP
rs71708944	998	dbSNP
rs1307209808	1004	dbSNP
rs1415304063	1007	dbSNP
rs1360868508	1021	dbSNP
rs1174022676	1029	dbSNP
rs1413919698	1031	dbSNP
rs1408808684	1033	dbSNP
rs543016227	1034	dbSNP
rs1471746147	1035	dbSNP
rs1002593046	1038	dbSNP
rs1034123089	1040	dbSNP
rs1486937156	1044	dbSNP
rs1236296678	1048	dbSNP
rs1339883096	1056	dbSNP
rs761817967	1060	dbSNP
rs894315499	1061	dbSNP
rs1006060338	1079	dbSNP
rs1030541706	1081	dbSNP
rs1396699320	1085	dbSNP
rs1016590617	1089	dbSNP
rs954926014	1091	dbSNP
rs79412243	1098	dbSNP
rs972458375	1100	dbSNP
rs1030363029	1102	dbSNP
rs954751833	1115	dbSNP
rs1381087853	1119	dbSNP
rs1300940018	1120	dbSNP
rs1243793966	1126	dbSNP
rs1286550180	1129	dbSNP
rs1322700698	1153	dbSNP
rs910689805	1156	dbSNP
rs986215307	1158	dbSNP
rs60057196	1161	dbSNP
rs1214279856	1162	dbSNP
rs151253289	1166	dbSNP
rs112032901	1167	dbSNP
rs1181750531	1167	dbSNP
rs1483275701	1167	dbSNP
rs1248086723	1170	dbSNP
rs989686514	1172	dbSNP
rs914121165	1173	dbSNP
rs1253635000	1174	dbSNP
rs1422936894	1182	dbSNP
rs1267030680	1183	dbSNP
rs55643751	1189	dbSNP
rs1213931214	1190	dbSNP
rs945064278	1191	dbSNP
rs1310334245	1198	dbSNP
rs1046062618	1214	dbSNP
rs906460915	1218	dbSNP
rs71654258	1221	dbSNP
rs1381233109	1226	dbSNP
rs1388018281	1227	dbSNP
rs937887033	1230	dbSNP
rs767797798	1232	dbSNP
rs1041746369	1242	dbSNP
rs527855603	1249	dbSNP
rs547646657	1250	dbSNP
rs906887833	1251	dbSNP
rs1164581152	1256	dbSNP
rs1429601623	1262	dbSNP
rs938395646	1269	dbSNP
rs1055478787	1272	dbSNP
rs368983152	1273	dbSNP
rs1360503148	1274	dbSNP
rs888303987	1278	dbSNP
rs1006009648	1281	dbSNP
rs1016124218	1291	dbSNP
rs375341118	1292	dbSNP
rs1377234226	1293	dbSNP
rs1276615293	1300	dbSNP
rs897872835	1305	dbSNP
rs562664881	1312	dbSNP
rs775502261	1313	dbSNP
rs373457309	1321	dbSNP
rs376361119	1323	dbSNP
rs1388720404	1332	dbSNP
rs1378790688	1336	dbSNP
rs1030262047	1339	dbSNP
rs766787435	1340	dbSNP
rs754198294	1341	dbSNP
rs369789656	1347	dbSNP
rs954699693	1355	dbSNP
rs1007597353	1356	dbSNP
rs1429694618	1358	dbSNP
rs1017696451	1359	dbSNP
rs1388986377	1362	dbSNP
rs865781618	1373	dbSNP
rs189388607	1377	dbSNP
rs181794054	1378	dbSNP
rs760130926	1379	dbSNP
rs914085274	1387	dbSNP
rs967342315	1393	dbSNP
rs1253748924	1401	dbSNP
rs531684166	1410	dbSNP
rs982388975	1421	dbSNP
rs1253683087	1423	dbSNP
rs71654259	1425	dbSNP
rs1202879085	1446	dbSNP
rs1341428847	1455	dbSNP
rs928270749	1457	dbSNP
rs1017842794	1458	dbSNP
rs569550326	1460	dbSNP
rs1482645172	1473	dbSNP
rs1198840966	1479	dbSNP
rs185879676	1483	dbSNP
rs552286668	1486	dbSNP
rs1239644459	1492	dbSNP
rs1235601353	1508	dbSNP
rs765642467	1515	dbSNP
rs565910589	1520	dbSNP
rs1055428744	1522	dbSNP
rs1392211435	1526	dbSNP
rs1352661265	1529	dbSNP
rs1306613693	1531	dbSNP
rs1409714854	1537	dbSNP
rs1376664772	1540	dbSNP
rs534836523	1557	dbSNP
rs913657389	1565	dbSNP
rs1407890249	1569	dbSNP
rs941814994	1574	dbSNP
rs1037911309	1575	dbSNP
rs897658259	1577	dbSNP
rs993785915	1582	dbSNP
rs1469386259	1587	dbSNP
rs554434864	1602	dbSNP
rs370594585	1609	dbSNP
rs937679440	1619	dbSNP
rs1402776883	1623	dbSNP
rs890414395	1625	dbSNP
rs536941595	1631	dbSNP
rs542060280	1634	dbSNP
rs576012424	1638	dbSNP
rs1036831646	1640	dbSNP
rs958079046	1645	dbSNP
rs1336641999	1654	dbSNP
rs1394835185	1656	dbSNP
rs1399376377	1671	dbSNP
rs1301805117	1672	dbSNP
rs1457415370	1682	dbSNP
rs1413810593	1691	dbSNP
rs1299135125	1695	dbSNP
rs897928523	1708	dbSNP
rs1368839417	1713	dbSNP
rs993521191	1713	dbSNP
rs545123899	1719	dbSNP
rs1422034054	1720	dbSNP
rs1255514065	1722	dbSNP
rs1051780377	1729	dbSNP
rs1259563013	1733	dbSNP
rs1011008795	1735	dbSNP
rs1021502672	1736	dbSNP
rs1018313764	1746	dbSNP
rs565057172	1751	dbSNP
rs966913099	1756	dbSNP
rs958276640	1759	dbSNP
rs750117568	1760	dbSNP
rs1340444043	1762	dbSNP
rs755819793	1766	dbSNP
rs1313732294	1771	dbSNP
rs1435262713	1776	dbSNP
rs116524098	1780	dbSNP
rs147857060	1781	dbSNP
rs749066760	1784	dbSNP
rs1480140553	1788	dbSNP
rs1164822807	1789	dbSNP
rs367726161	1790	dbSNP
rs1367878938	1797	dbSNP
rs966849748	1799	dbSNP
rs1451334737	1806	dbSNP
rs981886686	1817	dbSNP
rs561279911	1819	dbSNP
rs1464207281	1820	dbSNP
rs959335974	1821	dbSNP
rs1292432083	1826	dbSNP
rs990458777	1832	dbSNP
rs909797208	1835	dbSNP
rs530474004	1847	dbSNP
rs141432140	1848	dbSNP
rs1438385505	1849	dbSNP
rs563309109	1851	dbSNP
rs553441030	1852	dbSNP
rs1052054094	1854	dbSNP
rs1331391436	1863	dbSNP
rs1325260643	1867	dbSNP
rs1403330820	1869	dbSNP
rs1364740244	1884	dbSNP
rs933815421	1887	dbSNP
rs1431574927	1890	dbSNP
rs1051831088	1892	dbSNP
rs1388378373	1894	dbSNP
rs1168083039	1895	dbSNP
rs1478477839	1900	dbSNP
rs890365103	1903	dbSNP
rs1007494381	1906	dbSNP
rs552174801	1918	dbSNP
rs1451329394	1922	dbSNP
rs1253033832	1924	dbSNP
rs1007976191	1934	dbSNP
rs1271848862	1937	dbSNP
rs1213536654	1950	dbSNP
rs1315535542	1952	dbSNP
rs772056883	1956	dbSNP
rs146986590	1967	dbSNP
rs190351518	1969	dbSNP
rs115972876	1972	dbSNP
rs1308461836	1974	dbSNP
rs1411553158	1978	dbSNP
rs760954195	1981	dbSNP
rs1245475330	1983	dbSNP
rs1003698096	1989	dbSNP
rs1490349842	1992	dbSNP
rs1195044260	1993	dbSNP
rs568152367	1995	dbSNP
rs1265357994	2000	dbSNP
rs1035182227	2015	dbSNP
rs1171001635	2016	dbSNP
rs537385819	2017	dbSNP
rs966902149	2019	dbSNP
rs1184866007	2024	dbSNP
rs1167517101	2026	dbSNP
rs1482351588	2030	dbSNP
rs1003708838	2033	dbSNP
rs1406856259	2034	dbSNP
rs991051010	2043	dbSNP
rs1364881590	2045	dbSNP
rs959116682	2046	dbSNP
rs1217092968	2047	dbSNP
rs1322446429	2055	dbSNP
rs111687331	2061	dbSNP
rs556951588	2063	dbSNP
rs1022551201	2070	dbSNP
rs1288743909	2074	dbSNP
rs1230067035	2075	dbSNP
rs1348415856	2077	dbSNP
rs963067451	2084	dbSNP
rs961161121	2087	dbSNP
rs1299975101	2091	dbSNP
rs570382358	2096	dbSNP
rs538662774	2101	dbSNP
rs918993195	2102	dbSNP
rs1429448379	2114	dbSNP
rs1284388176	2117	dbSNP
rs929075634	2120	dbSNP
rs1161664334	2121	dbSNP
rs1471774875	2131	dbSNP
rs1407753869	2134	dbSNP
rs1182901781	2143	dbSNP
rs972655647	2161	dbSNP
rs987273806	2172	dbSNP
rs1207495696	2176	dbSNP
rs1460590793	2177	dbSNP
rs911745585	2185	dbSNP
rs943226628	2201	dbSNP
rs918497654	2208	dbSNP
rs115614064	2209	dbSNP
rs1326769791	2211	dbSNP
rs1230388228	2215	dbSNP
rs986557675	2218	dbSNP
rs1288088749	2221	dbSNP
rs911973048	2233	dbSNP
rs1451928928	2238	dbSNP
rs1217932022	2244	dbSNP
rs1288822423	2245	dbSNP
rs1335059567	2246	dbSNP
rs181538675	2249	dbSNP
rs1487332188	2252	dbSNP
rs1192623580	2262	dbSNP
rs1455861725	2265	dbSNP
rs1238256295	2267	dbSNP
rs1165977801	2274	dbSNP
rs943439864	2275	dbSNP
rs1180340143	2280	dbSNP
rs541164903	2281	dbSNP
rs1443409631	2286	dbSNP
rs946736088	2291	dbSNP
rs1042807127	2294	dbSNP
rs1420101891	2298	dbSNP
rs1190055700	2300	dbSNP
rs1484719604	2307	dbSNP
rs902586410	2308	dbSNP
rs759841882	2312	dbSNP
rs376465495	2313	dbSNP
rs1042697831	2314	dbSNP
rs78939910	2316	dbSNP
rs1299976411	2317	dbSNP
rs1334322339	2318	dbSNP
rs186532688	2320	dbSNP
rs1433130363	2321	dbSNP
rs1361608119	2324	dbSNP
rs1012401472	2326	dbSNP
rs368215836	2327	dbSNP
rs963311525	2328	dbSNP
rs1438982983	2339	dbSNP
rs1348507071	2353	dbSNP
rs1163465621	2365	dbSNP
rs973078023	2370	dbSNP
rs747621577	2372	dbSNP
rs553910204	2373	dbSNP
rs894908726	2374	dbSNP
rs1012023858	2377	dbSNP
rs1260532363	2380	dbSNP
rs544124355	2384	dbSNP
rs1388979746	2388	dbSNP
rs753151801	2394	dbSNP
rs879909866	2407	dbSNP
rs1266535328	2415	dbSNP
rs950473717	2416	dbSNP
rs1342833565	2425	dbSNP
rs1276859038	2437	dbSNP
rs115888574	2439	dbSNP
rs1302164485	2443	dbSNP
rs1025519897	2450	dbSNP
rs911694966	2455	dbSNP
rs955258682	2461	dbSNP
rs569065152	2464	dbSNP
rs545823815	2465	dbSNP
rs1373023551	2466	dbSNP
rs191342737	2468	dbSNP
rs1306728609	2478	dbSNP
rs538168706	2484	dbSNP
rs1372028767	2498	dbSNP
rs1204627871	2501	dbSNP
rs986610061	2510	dbSNP
rs1279339140	2528	dbSNP
rs911079923	2532	dbSNP
rs915144245	2545	dbSNP
rs528229615	2547	dbSNP
rs1242793191	2553	dbSNP
rs547960825	2555	dbSNP
rs1042365570	2557	dbSNP
rs1483334933	2559	dbSNP
rs1253814621	2561	dbSNP
rs1194011817	2572	dbSNP
rs902531843	2575	dbSNP
rs974888759	2578	dbSNP
rs1256689743	2584	dbSNP
rs374341971	2590	dbSNP
rs1321727250	2597	dbSNP
rs1180325008	2615	dbSNP
rs1229328641	2619	dbSNP
rs568273072	2621	dbSNP
rs1378404290	2624	dbSNP
rs1056465352	2626	dbSNP
rs1352715235	2630	dbSNP
rs895273465	2636	dbSNP
rs1286953745	2651	dbSNP
rs1012349013	2655	dbSNP
rs530637675	2658	dbSNP
rs866289347	2670	dbSNP
rs946700462	2675	dbSNP
rs1403661655	2676	dbSNP
rs1302961731	2678	dbSNP
rs1467256562	2685	dbSNP
rs1412291738	2696	dbSNP
rs777469819	2706	dbSNP
rs749165344	2707	dbSNP
rs1413947355	2715	dbSNP
rs1179511695	2723	dbSNP
rs1256918984	2729	dbSNP
rs555211916	2729	dbSNP
rs1400639710	2733	dbSNP
rs1202320919	2734	dbSNP
rs1447968253	2756	dbSNP
rs994845489	2758	dbSNP
rs1262666309	2766	dbSNP
rs1334356813	2767	dbSNP
rs550323594	2773	dbSNP
rs1238919467	2774	dbSNP
rs570421673	2781	dbSNP
rs939558929	2799	dbSNP
rs539299225	2803	dbSNP
rs1312789719	2807	dbSNP
rs1397208976	2811	dbSNP
rs1379949140	2812	dbSNP
rs1335628496	2821	dbSNP
rs950441608	2825	dbSNP
rs1390539491	2827	dbSNP
rs1156638458	2842	dbSNP
rs1452966570	2849	dbSNP
rs558576695	2856	dbSNP
rs1369049184	2857	dbSNP
rs895347946	2864	dbSNP
rs1442811846	2881	dbSNP
rs1008602994	2885	dbSNP
rs565927526	2894	dbSNP
rs1466450924	2902	dbSNP
rs964481200	2925	dbSNP
rs974561779	2940	dbSNP
rs770965500	2953	dbSNP
rs1016725495	2954	dbSNP
rs535157740	2965	dbSNP
rs147603112	2968	dbSNP
rs1357511812	2969	dbSNP
rs993802624	2971	dbSNP
rs1262721054	2972	dbSNP
rs1313804879	2974	dbSNP
rs1458425426	2975	dbSNP
rs574848502	2981	dbSNP
rs1236075708	2982	dbSNP
rs955310875	2990	dbSNP
rs1381389095	2994	dbSNP
rs1391221262	2996	dbSNP
rs967970452	3006	dbSNP
rs1199443358	3020	dbSNP
rs1164872517	3024	dbSNP
rs978417766	3039	dbSNP
rs143061766	3040	dbSNP
rs939351443	3053	dbSNP
rs963950477	3056	dbSNP
rs1240516722	3061	dbSNP
rs1192752797	3072	dbSNP
rs377406611	3075	dbSNP
rs1269943498	3084	dbSNP
rs1220879299	3092	dbSNP
rs1290396582	3095	dbSNP
rs1228582172	3097	dbSNP
rs182700972	3098	dbSNP
rs1268000555	3099	dbSNP
rs968093650	3101	dbSNP
rs916648465	3108	dbSNP
rs753487420	3109	dbSNP
rs1304771453	3113	dbSNP
rs1403021573	3118	dbSNP
rs1394720254	3124	dbSNP
rs1391207523	3133	dbSNP
rs978103924	3141	dbSNP
rs1417487432	3143	dbSNP
rs1167515650	3146	dbSNP
rs1324745272	3151	dbSNP
rs1379709805	3156	dbSNP
rs1197784291	3159	dbSNP
rs1490327548	3161	dbSNP
rs754732636	3168	dbSNP
rs1038512116	3184	dbSNP
rs1324386182	3186	dbSNP
rs923963596	3187	dbSNP
rs898661942	3189	dbSNP
rs1333378231	3213	dbSNP
rs778710828	3222	dbSNP
rs1270328226	3232	dbSNP
rs994394930	3235	dbSNP
rs1047753765	3237	dbSNP
rs1194856593	3245	dbSNP
rs1345354221	3249	dbSNP
rs1284955776	3262	dbSNP
rs1221391597	3278	dbSNP
rs148216118	3280	dbSNP
rs1288855117	3281	dbSNP
rs745700646	3285	dbSNP
rs1008550500	3289	dbSNP
rs201845381	3300	dbSNP
rs200021730	3302	dbSNP
rs34636426	3302	dbSNP
rs397864167	3302	dbSNP
rs948248932	3304	dbSNP
rs1415239807	3310	dbSNP
rs563973685	3326	dbSNP
rs1249867662	3330	dbSNP
rs964430997	3347	dbSNP
rs1480278755	3355	dbSNP
rs187729887	3356	dbSNP
rs748093321	3357	dbSNP
rs191108633	3358	dbSNP
rs890825778	3363	dbSNP
rs115207699	3372	dbSNP
rs1180557266	3373	dbSNP
rs1018193962	3374	dbSNP
rs964271044	3376	dbSNP
rs1263205242	3379	dbSNP
rs866867565	3391	dbSNP
rs1168043774	3394	dbSNP
rs1268571456	3398	dbSNP
rs1238827930	3400	dbSNP
rs968296368	3405	dbSNP
rs977988305	3409	dbSNP
rs923833857	3412	dbSNP
rs1372183627	3417	dbSNP
rs1461775500	3421	dbSNP
rs1164253609	3430	dbSNP
rs960638051	3435	dbSNP
rs1021443969	3452	dbSNP
rs1469522696	3457	dbSNP
rs967184817	3464	dbSNP
rs978202006	3467	dbSNP
rs541778137	3475	dbSNP
rs1399463451	3477	dbSNP
rs924014670	3479	dbSNP
rs992088960	3482	dbSNP
rs1182967296	3484	dbSNP
rs916599108	3492	dbSNP
rs1244478799	3498	dbSNP
rs1181336259	3509	dbSNP
rs948099350	3513	dbSNP
rs561641178	3515	dbSNP
rs1248392997	3524	dbSNP
rs1217759429	3530	dbSNP
rs1314910945	3557	dbSNP
rs1278825580	3566	dbSNP
rs992530040	3571	dbSNP
rs1401972769	3575	dbSNP
rs1293674855	3576	dbSNP
rs1361645571	3576	dbSNP
rs1313075633	3578	dbSNP
rs1326131941	3578	dbSNP
rs1458147238	3579	dbSNP
rs1290861916	3580	dbSNP
rs1344996358	3580	dbSNP
rs1428993062	3580	dbSNP
rs1452178148	3580	dbSNP
rs113168124	3581	dbSNP
rs1161192291	3581	dbSNP
rs1187913568	3581	dbSNP
rs1427153107	3581	dbSNP
rs1443624834	3581	dbSNP
rs1484740852	3581	dbSNP
rs35987024	3581	dbSNP
rs542705326	3581	dbSNP
rs551817094	3581	dbSNP
rs555028694	3581	dbSNP
rs869192932	3581	dbSNP
rs1245240297	3582	dbSNP
rs75706174	3583	dbSNP
rs1266920447	3585	dbSNP
rs1357023989	3586	dbSNP
rs1245256541	3587	dbSNP
rs796310037	3587	dbSNP
rs1221589026	3588	dbSNP
rs868755190	3589	dbSNP
rs920055911	3590	dbSNP
rs1488966016	3591	dbSNP
rs930133308	3592	dbSNP
rs1047251451	3593	dbSNP
rs201384913	3594	dbSNP
rs891371310	3594	dbSNP
rs1221688317	3595	dbSNP
rs944285126	3595	dbSNP
rs1040040961	3596	dbSNP
rs869045521	3596	dbSNP
rs75806897	3597	dbSNP
rs113754207	3598	dbSNP
rs1022457209	3599	dbSNP
rs1361794342	3600	dbSNP
rs1421579348	3601	dbSNP
rs1287256285	3602	dbSNP
rs1411002406	3607	dbSNP
rs1396548387	3610	dbSNP
rs903623497	3616	dbSNP
rs999752546	3627	dbSNP
rs1353496924	3630	dbSNP
rs1176729272	3642	dbSNP
rs1470805947	3652	dbSNP
rs117615366	3686	dbSNP
rs960586161	3694	dbSNP
rs1315134615	3698	dbSNP
rs992039750	3701	dbSNP
rs145239035	3706	dbSNP
rs1419652714	3707	dbSNP
rs1252738587	3709	dbSNP
rs969351988	3710	dbSNP
rs1175622901	3711	dbSNP
rs775661814	3722	dbSNP
rs899766080	3723	dbSNP
rs368591663	3724	dbSNP
rs1321801631	3726	dbSNP
rs374247688	3738	dbSNP
rs546477921	3746	dbSNP
rs1355836445	3747	dbSNP
rs1021540873	3754	dbSNP
rs368289829	3755	dbSNP
rs1372010935	3756	dbSNP
rs983326198	3759	dbSNP
rs1201781671	3760	dbSNP
rs374493415	3767	dbSNP
rs566096753	3779	dbSNP
rs377091538	3780	dbSNP
rs912755270	3794	dbSNP
rs534730510	3807	dbSNP
rs944234280	3808	dbSNP
rs992187207	3810	dbSNP
rs1024035489	3816	dbSNP
rs1243853668	3828	dbSNP
rs1185617575	3840	dbSNP
rs1039988783	3841	dbSNP
rs969398878	3843	dbSNP
rs900121516	3844	dbSNP
rs931620278	3845	dbSNP
rs1259441588	3852	dbSNP
rs548528823	3853	dbSNP
rs903569947	3856	dbSNP
rs1352295894	3859	dbSNP
rs1315202000	3865	dbSNP
rs1002777390	3874	dbSNP
rs1380206438	3876	dbSNP
rs920173304	3877	dbSNP
rs1382516347	3884	dbSNP
rs1466643989	3889	dbSNP
rs1287003171	3896	dbSNP
rs930145818	3897	dbSNP
rs1385913669	3900	dbSNP
rs1404245922	3901	dbSNP
rs534226145	3915	dbSNP
rs1442865720	3917	dbSNP
rs1384783988	3922	dbSNP
rs1194200046	3923	dbSNP
rs113498235	3932	dbSNP
rs537497214	3933	dbSNP
rs557628032	3938	dbSNP
rs1490673220	3942	dbSNP
rs577496098	3949	dbSNP
rs1247446948	3969	dbSNP
rs1306244339	3976	dbSNP
rs1283187959	3981	dbSNP
rs560751288	3982	dbSNP
rs11308713	3983	dbSNP
rs1175786883	3983	dbSNP
rs1293232173	3983	dbSNP
rs1327945563	3983	dbSNP
rs1422771717	3983	dbSNP
rs397757095	3983	dbSNP
rs200770785	3984	dbSNP
rs201899417	3988	dbSNP
rs200482586	3989	dbSNP
rs1371688353	3991	dbSNP
rs1193004792	3994	dbSNP
rs1480970621	3995	dbSNP
rs1039436091	3996	dbSNP
rs1220930308	3998	dbSNP
rs1489614663	3998	dbSNP
rs1195995882	3999	dbSNP
rs899524994	3999	dbSNP
rs1341104660	4005	dbSNP
rs1234847897	4009	dbSNP
rs112002286	4010	dbSNP
rs1217732657	4011	dbSNP
rs1460649545	4017	dbSNP
rs969356294	4026	dbSNP
rs540164593	4033	dbSNP
rs138498466	4035	dbSNP
rs1027001765	4038	dbSNP
rs573446621	4041	dbSNP
rs903063224	4051	dbSNP
rs951523371	4053	dbSNP
rs541467013	4055	dbSNP
rs912702764	4068	dbSNP
rs747070367	4069	dbSNP
rs760937718	4070	dbSNP
rs561677106	4074	dbSNP
rs1250485471	4079	dbSNP
rs1470479410	4081	dbSNP
rs1431941633	4085	dbSNP
rs998696861	4086	dbSNP
rs1035496683	4090	dbSNP
rs1245610038	4093	dbSNP
rs183216869	4094	dbSNP

- Genes & development, 2013

When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.