pre-miRNA Information
pre-miRNA hsa-mir-4708   
Genomic Coordinates chr14: 65335117 - 65335183
Description Homo sapiens miR-4708 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4708-5p
Sequence 9| AGAGAUGCCGCCUUGCUCCUU |29
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1017378847 9 dbSNP
rs750825813 10 dbSNP
rs1297395738 19 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol CCR6   
Synonyms BN-1, C-C CKR-6, CC-CKR-6, CCR-6, CD196, CKR-L3, CKRL3, CMKBR6, DCR2, DRY6, GPR29, GPRCY4, STRL22
Description C-C motif chemokine receptor 6
Transcript NM_004367   
Other Transcripts NM_031409   
Expression
Putative miRNA Targets on CCR6
3'UTR of CCR6
(miRNA target sites are highlighted)
>CCR6|NM_004367|3'UTR
   1 TAGAAAGCTGAGTCTCCCTAAGGCATGTGTGAAACATACTCATAGATGTTATGCAAAAAAAAGTCTATGGCCAGGTATGC
  81 ATGGAAAATGTGGGAATTAAGCAAAATCAAGCAAGCCTCTCTCCTGCGGGACTTAACGTGCTCATGGGCTGTGTGATCTC
 161 TTCAGGGTGGGGTGGTCTCTGATAGGTAGCATTTTCCAGCACTTTGCAAGGAATGTTTTGTAGCTCTAGGGTATATATCC
 241 GCCTGGCATTTCACAAAACAGCCTTTGGGAAATGCTGAATTAAAGTGAATTGTTGACAAATGTAAACATTTTCAGAAATA
 321 TTCATGAAGCGGTCACAGATCACAGTGTCTTTTGGTTACAGCACAAAATGATGGCAGTGGTTTGAAAAACTAAAACAGAA
 401 AAAAAAATGGAAGCCAACACATCACTCATTTTAGGCAAATGTTTAAACATTTTTATCTATCAGAATGTTTATTGTTGCTG
 481 GTTATAAGCAGCAGGATTGGCCGGCTAGTGTTTCCTCTCATTTCCCTTTGATACAGTCAACAAGCCTGACCCTGTAAAAT
 561 GGAGGTGGAAAGACAAGCTCAAGTGTTCACAACCTGGAAGTGCTTCGGGAAGAAGGGGACAATGGCAGAACAGGTGTTGG
 641 TGACAATTGTCACCAATTGGATAAAGCAGCTCAGGTTGTAGTGGGCCATTAGGAAACTGTCGGTTTGCTTTGATTTCCCT
 721 GGGAGCTGTTCTCTGTCGTGAGTGTCTCTTGTCTAAACGTCCATTAAGCTGAGAGTGCTATGAAGACAGGATCTAGAATA
 801 ATCTTGCTCACAGCTGTGCTCTGAGTGCCTAGCGGAGTTCCAGCAAACAAAATGGACTCAAGAGAGATTTGATTAATGAA
 881 TCGTAATGAAGTTGGGGTTTATTGTACAGTTTAAAATGTTAGATGTTTTTAATTTTTTAAATAAATGGAATACTTTTTTT
 961 TTTTTTTTAAAGAAAGCAACTTTACTGAGACAATGTAGAAAGAAGTTTTGTTCCGTTTCTTTAATGTGGTTGAAGAGCAA
1041 TGTGTGGCTGAAGACTTTTGTTATGAGGAGCTGCAGATTAGCTAGGGGACAGCTGGAATTATGCTGGCTTCTGATAATTA
1121 TTTTAAAGGGGTCTGAAATTTGTGATGGAATCAGATTTTAACAGCTCTCTTCAATGACATAGAAAGTTCATGGAACTCAT
1201 GTTTTTAAAGGGCTATGTAAATATATGAACATTAGAAAAATAGCAACTTGTGTTACAAAAATACAAACACATGTTAGGAA
1281 GGTACTGTCATGGGCTAGGCATGGTGGCTCACACCTGTAATCCCAGCATTTTGGGAAGCTAAGATGGGTGGATCACTTGA
1361 GGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGCGAAACCCCTCTCTACTAAAAATACAAAAATTTGCCAGGCGTGGTG
1441 GCGGGTGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAAGAGAATCGCTTGAACCCAGGAGGCAGAGGTTGCAGTGAG
1521 CCGAGATCGTGCCATTGCACTCCAGCCTGGGTGACAAAGCGAGACTCCATCTCAAAAAAAAAAAAAAAAAAAAAGGAAAG
1601 AACTGTCATGTAAACATACCAACATGTTTAAACCTGACAATGGTGTTATTTGAAACTTTATATTGTTCTTGTAAGCTTTA
1681 ACTATATCTCTCTTTAAAATGCAAAATAATGTCTTAAGATTCAAAGTCTGTATTTTTAAAGCATGGCTTTGGCTTTGCAA
1761 AATAAAAAATGTGTTTTGTACATGAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' uuccUCGUUCCGCCGUAGAGa 5'
              |||:|| |  |||||| 
Target 5' acaaAGCGAGACTCCATCTCa 3'
1554 - 1574 133.00 -13.70
2
miRNA  3' uuccucguuccGCCGUAGAGa 5'
                     :|||||:|| 
Target 5' atatatccgccTGGCATTTCa 3'
233 - 253 130.00 -10.30
3
miRNA  3' uuccUCGUUCCGCCGUAGAGa 5'
              | ||||   || |||| 
Target 5' caaaATCAAGCAAGCCTCTCt 3'
102 - 122 121.00 -9.60
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30463414 19 COSMIC
COSN30452677 21 COSMIC
COSN30462223 32 COSMIC
COSN15664116 63 COSMIC
COSN30140994 72 COSMIC
COSN31549921 75 COSMIC
COSN30156180 76 COSMIC
COSN31494034 81 COSMIC
COSN31555486 89 COSMIC
COSN8513707 102 COSMIC
COSN32077960 139 COSMIC
COSN28790900 141 COSMIC
COSN28148888 180 COSMIC
COSN29358320 226 COSMIC
COSN32062645 589 COSMIC
COSN32064221 804 COSMIC
COSN20103645 968 COSMIC
COSN20103643 969 COSMIC
COSN26403079 1444 COSMIC
COSN9501825 1515 COSMIC
COSN2153321 1760 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1405277610 1 dbSNP
rs757970953 2 dbSNP
rs149010509 3 dbSNP
rs367637187 8 dbSNP
rs371334790 9 dbSNP
rs774136024 15 dbSNP
rs375979417 27 dbSNP
rs368305564 28 dbSNP
rs1236105990 40 dbSNP
rs1472732336 42 dbSNP
rs750367865 44 dbSNP
rs777781807 46 dbSNP
rs1382748999 47 dbSNP
rs1437891185 49 dbSNP
rs373043902 53 dbSNP
rs533011104 54 dbSNP
rs1181327619 55 dbSNP
rs1486256107 55 dbSNP
rs551258713 56 dbSNP
rs1207007536 57 dbSNP
rs950059260 59 dbSNP
rs933190177 61 dbSNP
rs1047555947 68 dbSNP
rs1234984057 69 dbSNP
rs1319841141 72 dbSNP
rs55838414 79 dbSNP
rs886381225 83 dbSNP
rs1311984652 87 dbSNP
rs1214616804 90 dbSNP
rs958228368 99 dbSNP
rs1363404465 102 dbSNP
rs200469053 103 dbSNP
rs1006105474 106 dbSNP
rs927351962 107 dbSNP
rs988260098 113 dbSNP
rs191972938 120 dbSNP
rs1017365981 128 dbSNP
rs1459810674 129 dbSNP
rs1367200921 131 dbSNP
rs777260856 138 dbSNP
rs566693748 139 dbSNP
rs1392040975 142 dbSNP
rs1186764480 145 dbSNP
rs886270348 146 dbSNP
rs1253934682 158 dbSNP
rs1023089234 164 dbSNP
rs1004833485 168 dbSNP
rs971468356 171 dbSNP
rs1040658378 172 dbSNP
rs1294759257 177 dbSNP
rs533971801 179 dbSNP
rs1371248928 184 dbSNP
rs1305225898 185 dbSNP
rs1031826754 188 dbSNP
rs1384744324 197 dbSNP
rs35695660 210 dbSNP
rs746856512 211 dbSNP
rs1305495272 212 dbSNP
rs990395012 218 dbSNP
rs548930998 226 dbSNP
rs1462888142 227 dbSNP
rs1166786210 231 dbSNP
rs1373023470 231 dbSNP
rs943006046 232 dbSNP
rs996219402 235 dbSNP
rs770791820 236 dbSNP
rs1395887937 240 dbSNP
rs1201230009 241 dbSNP
rs41411246 242 dbSNP
rs1266324710 249 dbSNP
rs1196227404 253 dbSNP
rs1483987328 258 dbSNP
rs923065966 262 dbSNP
rs368123608 263 dbSNP
rs139105246 277 dbSNP
rs41501544 278 dbSNP
rs886247876 296 dbSNP
rs879232056 301 dbSNP
rs1033219608 310 dbSNP
rs143957696 325 dbSNP
rs1330156465 331 dbSNP
rs941943040 332 dbSNP
rs538687486 336 dbSNP
rs554440150 337 dbSNP
rs1352849622 341 dbSNP
rs1306762229 350 dbSNP
rs1429708390 379 dbSNP
rs956396296 383 dbSNP
rs572685704 394 dbSNP
rs1376096673 396 dbSNP
rs1174404319 397 dbSNP
rs1292445933 397 dbSNP
rs1464726958 399 dbSNP
rs199864702 399 dbSNP
rs554563917 408 dbSNP
rs6931699 409 dbSNP
rs1248508508 412 dbSNP
rs753426256 418 dbSNP
rs1182817008 420 dbSNP
rs188648743 421 dbSNP
rs927278473 422 dbSNP
rs930767595 425 dbSNP
rs543513205 429 dbSNP
rs1261002999 430 dbSNP
rs1229360774 431 dbSNP
rs1331619809 437 dbSNP
rs1286872952 440 dbSNP
rs1165810591 456 dbSNP
rs1341658750 458 dbSNP
rs745643756 460 dbSNP
rs1386460773 463 dbSNP
rs1429638142 466 dbSNP
rs1401729729 472 dbSNP
rs1165719318 475 dbSNP
rs1049124852 481 dbSNP
rs1032275659 485 dbSNP
rs956195223 495 dbSNP
rs1158151975 498 dbSNP
rs1475493732 499 dbSNP
rs1239200492 502 dbSNP
rs1012252013 503 dbSNP
rs17860854 504 dbSNP
rs1380067308 515 dbSNP
rs74592470 516 dbSNP
rs1441383195 519 dbSNP
rs1261857312 520 dbSNP
rs1454423575 525 dbSNP
rs940478403 527 dbSNP
rs1040308726 535 dbSNP
rs1266556340 542 dbSNP
rs532363199 542 dbSNP
rs1315439793 548 dbSNP
rs1357641844 554 dbSNP
rs901730232 566 dbSNP
rs1176553172 569 dbSNP
rs1364459319 574 dbSNP
rs1226072568 585 dbSNP
rs1243293209 594 dbSNP
rs1339892555 602 dbSNP
rs1321313783 604 dbSNP
rs541101238 607 dbSNP
rs1050113782 608 dbSNP
rs3093006 610 dbSNP
rs1415836105 621 dbSNP
rs527610365 622 dbSNP
rs1000651818 632 dbSNP
rs1196415856 650 dbSNP
rs748033664 652 dbSNP
rs1189949566 654 dbSNP
rs41409048 658 dbSNP
rs954631725 660 dbSNP
rs891967008 667 dbSNP
rs180999088 670 dbSNP
rs1452507087 671 dbSNP
rs907707530 676 dbSNP
rs528577100 682 dbSNP
rs1037536754 683 dbSNP
rs1449451597 686 dbSNP
rs980318764 693 dbSNP
rs1230781685 702 dbSNP
rs1363262478 703 dbSNP
rs774950476 707 dbSNP
rs1297194707 710 dbSNP
rs1191676352 717 dbSNP
rs1375214183 727 dbSNP
rs1462077320 738 dbSNP
rs916524706 738 dbSNP
rs549900554 739 dbSNP
rs547158812 747 dbSNP
rs41331945 748 dbSNP
rs1169911539 749 dbSNP
rs965603541 752 dbSNP
rs41376751 759 dbSNP
rs1193819766 778 dbSNP
rs1364213981 781 dbSNP
rs1380246432 790 dbSNP
rs1297676901 792 dbSNP
rs1451523096 806 dbSNP
rs189354587 808 dbSNP
rs536560287 819 dbSNP
rs3093005 831 dbSNP
rs1011788376 833 dbSNP
rs746764726 834 dbSNP
rs1272857199 835 dbSNP
rs1435557615 838 dbSNP
rs1350996215 843 dbSNP
rs140165498 853 dbSNP
rs1263926771 858 dbSNP
rs543650060 867 dbSNP
rs558610397 868 dbSNP
rs1203712652 872 dbSNP
rs1029954142 882 dbSNP
rs182173696 883 dbSNP
rs1050328108 884 dbSNP
rs1429600667 891 dbSNP
rs187212069 893 dbSNP
rs1426579946 894 dbSNP
rs1477929416 897 dbSNP
rs936284311 897 dbSNP
rs1481101386 898 dbSNP
rs1184906263 903 dbSNP
rs907654975 904 dbSNP
rs76436762 905 dbSNP
rs1210998079 913 dbSNP
rs1314153373 931 dbSNP
rs1276821208 933 dbSNP
rs1218891902 935 dbSNP
rs1329536530 936 dbSNP
rs559501180 945 dbSNP
rs41322047 947 dbSNP
rs1349867005 950 dbSNP
rs973261402 950 dbSNP
rs1413088186 954 dbSNP
rs1457463949 954 dbSNP
rs35220458 954 dbSNP
rs369925665 954 dbSNP
rs536636155 954 dbSNP
rs58535245 954 dbSNP
rs67363454 954 dbSNP
rs764558623 954 dbSNP
rs79569135 954 dbSNP
rs55796656 964 dbSNP
rs916389725 965 dbSNP
rs1451390184 969 dbSNP
rs1471895829 969 dbSNP
rs370035138 970 dbSNP
rs947970783 996 dbSNP
rs1013317184 1000 dbSNP
rs1484252205 1010 dbSNP
rs1386136055 1014 dbSNP
rs1046307596 1015 dbSNP
rs907360677 1016 dbSNP
rs569983083 1024 dbSNP
rs928635736 1036 dbSNP
rs1225353097 1038 dbSNP
rs1284196166 1051 dbSNP
rs938732197 1071 dbSNP
rs1313403398 1072 dbSNP
rs1229989828 1103 dbSNP
rs1220844604 1109 dbSNP
rs1301768767 1115 dbSNP
rs1435287098 1122 dbSNP
rs1390472440 1126 dbSNP
rs1237393048 1132 dbSNP
rs1053528438 1136 dbSNP
rs1322958624 1139 dbSNP
rs1262863766 1154 dbSNP
rs891917210 1168 dbSNP
rs947464261 1169 dbSNP
rs1486514265 1177 dbSNP
rs1166040882 1178 dbSNP
rs1209787247 1179 dbSNP
rs1043211717 1180 dbSNP
rs10946217 1185 dbSNP
rs1490706608 1186 dbSNP
rs1411629186 1197 dbSNP
rs1183138028 1211 dbSNP
rs757987475 1217 dbSNP
rs1199447931 1219 dbSNP
rs1410835064 1225 dbSNP
rs952063798 1226 dbSNP
rs1030571222 1236 dbSNP
rs763472427 1244 dbSNP
rs1004593094 1251 dbSNP
rs1223599967 1253 dbSNP
rs1319858943 1256 dbSNP
rs1311388565 1257 dbSNP
rs1228579116 1264 dbSNP
rs1014914817 1270 dbSNP
rs11575083 1271 dbSNP
rs1433929963 1271 dbSNP
rs116855733 1279 dbSNP
rs1294490994 1292 dbSNP
rs973110352 1308 dbSNP
rs531527941 1311 dbSNP
rs969181838 1316 dbSNP
rs986048423 1318 dbSNP
rs1430528630 1341 dbSNP
rs925168696 1344 dbSNP
rs550036935 1346 dbSNP
rs11575084 1350 dbSNP
rs1267707590 1351 dbSNP
rs191584399 1363 dbSNP
rs1487459613 1365 dbSNP
rs1291011774 1367 dbSNP
rs1205218374 1368 dbSNP
rs1343614357 1387 dbSNP
rs1271872880 1392 dbSNP
rs181542353 1396 dbSNP
rs745720012 1397 dbSNP
rs947494952 1415 dbSNP
rs755844205 1418 dbSNP
rs949191837 1419 dbSNP
rs1388937332 1427 dbSNP
rs780256313 1430 dbSNP
rs932176604 1435 dbSNP
rs768821373 1436 dbSNP
rs1410997695 1441 dbSNP
rs41472245 1443 dbSNP
rs11575085 1444 dbSNP
rs374914584 1446 dbSNP
rs1178588621 1452 dbSNP
rs1250709956 1454 dbSNP
rs1423056293 1458 dbSNP
rs1055983314 1462 dbSNP
rs1483995524 1466 dbSNP
rs1248976534 1479 dbSNP
rs1005511890 1482 dbSNP
rs888056770 1488 dbSNP
rs768799983 1489 dbSNP
rs898802553 1490 dbSNP
rs185625723 1492 dbSNP
rs1257432451 1493 dbSNP
rs1230645405 1500 dbSNP
rs1321798532 1510 dbSNP
rs1387061183 1523 dbSNP
rs994601684 1524 dbSNP
rs1306249775 1527 dbSNP
rs1164362025 1529 dbSNP
rs1304772283 1530 dbSNP
rs1464742309 1535 dbSNP
rs1354027994 1536 dbSNP
rs1428257604 1540 dbSNP
rs1159711152 1544 dbSNP
rs1460474350 1553 dbSNP
rs1409727835 1556 dbSNP
rs9366094 1558 dbSNP
rs1257684994 1560 dbSNP
rs11575086 1561 dbSNP
rs1452431294 1562 dbSNP
rs1157876556 1574 dbSNP
rs1184863114 1574 dbSNP
rs1213942869 1574 dbSNP
rs1278341324 1574 dbSNP
rs1320952688 1574 dbSNP
rs1369625854 1574 dbSNP
rs1386220401 1574 dbSNP
rs1424299578 1574 dbSNP
rs1443618925 1574 dbSNP
rs1458654468 1574 dbSNP
rs1473093800 1574 dbSNP
rs1491434028 1574 dbSNP
rs35977074 1574 dbSNP
rs1015379845 1575 dbSNP
rs962065709 1575 dbSNP
rs1466500554 1576 dbSNP
rs1313333821 1577 dbSNP
rs1282627419 1582 dbSNP
rs1385711815 1586 dbSNP
rs1204988414 1588 dbSNP
rs998019977 1588 dbSNP
rs1247330791 1595 dbSNP
rs1272850833 1596 dbSNP
rs1243929351 1598 dbSNP
rs1305261927 1601 dbSNP
rs1205959518 1603 dbSNP
rs1030377682 1609 dbSNP
rs1298554595 1610 dbSNP
rs1251549494 1619 dbSNP
rs534815245 1621 dbSNP
rs6456162 1622 dbSNP
rs1181566159 1625 dbSNP
rs1244734980 1639 dbSNP
rs981979868 1643 dbSNP
rs1035255995 1646 dbSNP
rs1240710800 1650 dbSNP
rs1192989038 1651 dbSNP
rs956655935 1653 dbSNP
rs1452668205 1657 dbSNP
rs1292690522 1660 dbSNP
rs1185866761 1661 dbSNP
rs1220941057 1666 dbSNP
rs1417254931 1670 dbSNP
rs1408299528 1672 dbSNP
rs985933487 1675 dbSNP
rs988421526 1676 dbSNP
rs1337771516 1679 dbSNP
rs1269366135 1688 dbSNP
rs1436994338 1691 dbSNP
rs913247856 1694 dbSNP
rs55701277 1703 dbSNP
rs542251187 1708 dbSNP
rs988067551 1710 dbSNP
rs1170009470 1711 dbSNP
rs142353378 1717 dbSNP
rs576436832 1718 dbSNP
rs1469480292 1723 dbSNP
rs761137457 1724 dbSNP
rs766784882 1725 dbSNP
rs1248840797 1731 dbSNP
rs1051945788 1742 dbSNP
rs1046587617 1756 dbSNP
rs1453750109 1764 dbSNP
rs1270161085 1769 dbSNP
rs926344805 1772 dbSNP
rs56240207 1777 dbSNP
rs1055914757 1783 dbSNP
rs1215862095 1786 dbSNP
rs1272616002 1787 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084044. RNA binding protein: AGO2. Condition:CLIP_noarsenite_rep3 ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' uuccucguuccgccGUAGAGa 5'
                        |||||| 
Target 5' ---------gacucCAUCUCa 3'
1 - 12
Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions Cardiac Tissues
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM2202477. RNA binding protein: AGO2. Condition:S2_LV_25yo_Male_AGO2_bound_RNA HITS-CLIP data was present in GSM2202481. RNA binding protein: AGO2. Condition:S6_LV_61yo_Male_AGO2_bound_RNA ...

- Spengler RM; Zhang X; Cheng C; McLendon JM; et al., 2016, Nucleic acids research.

Article - Spengler RM; Zhang X; Cheng C; McLendon JM; et al.
- Nucleic acids research, 2016
MicroRNAs (miRs) have emerged as key biological effectors in human health and disease. These small noncoding RNAs are incorporated into Argonaute (Ago) proteins, where they direct post-transcriptional gene silencing via base-pairing with target transcripts. Although miRs have become intriguing biological entities and attractive therapeutic targets, the translational impacts of miR research remain limited by a paucity of empirical miR targeting data, particularly in human primary tissues. Here, to improve our understanding of the diverse roles miRs play in cardiovascular function and disease, we applied high-throughput methods to globally profile miR:target interactions in human heart tissues. We deciphered Ago2:RNA interactions using crosslinking immunoprecipitation coupled with high-throughput sequencing (HITS-CLIP) to generate the first transcriptome-wide map of miR targeting events in human myocardium, detecting 4000 cardiac Ago2 binding sites across >2200 target transcripts. Our initial exploration of this interactome revealed an abundance of miR target sites in gene coding regions, including several sites pointing to new miR-29 functions in regulating cardiomyocyte calcium, growth and metabolism. Also, we uncovered several clinically-relevant interactions involving common genetic variants that alter miR targeting events in cardiomyopathy-associated genes. Overall, these data provide a critical resource for bolstering translational miR research in heart, and likely beyond.
LinkOut: [PMID: 27418678]
CLIP-seq Support 1 for dataset GSM4903829
Method / RBP HITS-CLIP / AGO
Cell line / Condition Human neurons / CTLTD_shCTL_a
Location of target site NM_004367 | 3UTR | GUUGCAGUGAGCCGAGAUCGUGCCAUUGCACUCCAGCCUGGGUGACAAAGCGAGACUCC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161238
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM4903829
Method / RBP HITS-CLIP / AGO
Cell line / Condition Human neurons / CTLTD_shCTL_a
Location of target site NM_004367 | 3UTR | CUCAAAAAAAAAAAAAAAAAAAAAGG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161238
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM4903830
Method / RBP HITS-CLIP / AGO
Cell line / Condition Human neurons / CTLTD_shCTL_b
Location of target site NM_004367 | 3UTR | CUCCAGCCUGGGUGACAAAGCGAGACUCC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE161238
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM1084044
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_noarsenite_rep3
Location of target site ENST00000341935.5 | 3UTR | GACUCCAUCUCAAAAAAAAAAAAAAAAAAAAAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
94 hsa-miR-4708-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT095681 RBM27 RNA binding motif protein 27 2 4
MIRT104033 USP42 ubiquitin specific peptidase 42 2 6
MIRT114773 CMPK1 cytidine/uridine monophosphate kinase 1 2 2
MIRT246923 CCND1 cyclin D1 2 2
MIRT392569 ORAI2 ORAI calcium release-activated calcium modulator 2 2 2
MIRT443949 LRIT3 leucine rich repeat, Ig-like and transmembrane domains 3 2 2
MIRT446695 PAPPA pappalysin 1 2 2
MIRT447383 VOPP1 vesicular, overexpressed in cancer, prosurvival protein 1 2 2
MIRT449321 FAM120AOS family with sequence similarity 120A opposite strand 2 2
MIRT449717 C1orf61 chromosome 1 open reading frame 61 2 2
MIRT449738 TAB2 TGF-beta activated kinase 1/MAP3K7 binding protein 2 2 2
MIRT450531 PGLS 6-phosphogluconolactonase 2 2
MIRT455650 YARS tyrosyl-tRNA synthetase 2 2
MIRT458036 MRPL12 mitochondrial ribosomal protein L12 2 2
MIRT463468 ZC3HAV1L zinc finger CCCH-type containing, antiviral 1 like 2 2
MIRT466677 TAF1D TATA-box binding protein associated factor, RNA polymerase I subunit D 2 4
MIRT467789 SLC2A14 solute carrier family 2 member 14 2 2
MIRT468167 SGPL1 sphingosine-1-phosphate lyase 1 2 2
MIRT468652 SECISBP2L SECIS binding protein 2 like 2 6
MIRT469380 RER1 retention in endoplasmic reticulum sorting receptor 1 2 2
MIRT470346 PPP2R5E protein phosphatase 2 regulatory subunit B'epsilon 2 2
MIRT472418 NCKAP1 NCK associated protein 1 2 2
MIRT474612 KLF3 Kruppel like factor 3 2 2
MIRT478703 CSRNP2 cysteine and serine rich nuclear protein 2 2 2
MIRT481008 BBC3 BCL2 binding component 3 2 4
MIRT483098 TFPI tissue factor pathway inhibitor 2 2
MIRT485113 SHISA6 shisa family member 6 2 2
MIRT497533 ZNF607 zinc finger protein 607 2 2
MIRT500644 TUBB2A tubulin beta 2A class IIa 2 6
MIRT500890 STRN striatin 2 4
MIRT501900 MED13 mediator complex subunit 13 2 2
MIRT506646 MAPK1 mitogen-activated protein kinase 1 2 4
MIRT512675 ENO4 enolase family member 4 2 2
MIRT516977 OR7D2 olfactory receptor family 7 subfamily D member 2 2 2
MIRT528754 RPS27 ribosomal protein S27 2 6
MIRT539670 ZBTB44 zinc finger and BTB domain containing 44 2 2
MIRT544129 PPIL1 peptidylprolyl isomerase like 1 2 2
MIRT546382 STOX2 storkhead box 2 2 4
MIRT562143 IGFBP5 insulin like growth factor binding protein 5 2 2
MIRT568713 TMEM30B transmembrane protein 30B 2 2
MIRT571029 CENPP centromere protein P 2 2
MIRT572781 ZNF277 zinc finger protein 277 2 2
MIRT573162 SLC30A9 solute carrier family 30 member 9 2 2
MIRT609126 NUDT3 nudix hydrolase 3 2 2
MIRT609284 OAS3 2'-5'-oligoadenylate synthetase 3 2 2
MIRT613430 GALNT6 polypeptide N-acetylgalactosaminyltransferase 6 2 2
MIRT613770 TTC38 tetratricopeptide repeat domain 38 2 2
MIRT616645 LRAT lecithin retinol acyltransferase 2 4
MIRT630892 SLC25A33 solute carrier family 25 member 33 2 2
MIRT636526 FAXC failed axon connections homolog 2 4
MIRT641394 NUBPL nucleotide binding protein like 2 2
MIRT641412 SCN2B sodium voltage-gated channel beta subunit 2 2 2
MIRT642528 CERS4 ceramide synthase 4 2 2
MIRT643186 HYPK huntingtin interacting protein K 2 2
MIRT647800 FRMD8 FERM domain containing 8 2 2
MIRT652150 TRIM71 tripartite motif containing 71 2 2
MIRT652602 TIMM8A translocase of inner mitochondrial membrane 8A 2 2
MIRT661606 C2orf15 chromosome 2 open reading frame 15 2 2
MIRT666339 SKAP2 src kinase associated phosphoprotein 2 2 2
MIRT670414 ELP2 elongator acetyltransferase complex subunit 2 2 2
MIRT671122 ZNF573 zinc finger protein 573 2 2
MIRT671155 ANKRD9 ankyrin repeat domain 9 2 2
MIRT671338 FAM71F2 family with sequence similarity 71 member F2 2 2
MIRT671869 ZNF429 zinc finger protein 429 2 2
MIRT671974 IKZF3 IKAROS family zinc finger 3 2 2
MIRT672064 KIAA0930 KIAA0930 2 2
MIRT672654 SLC25A16 solute carrier family 25 member 16 2 4
MIRT672673 GTF2H5 general transcription factor IIH subunit 5 2 2
MIRT672771 UBE2V2 ubiquitin conjugating enzyme E2 V2 2 2
MIRT672929 LRRC2 leucine rich repeat containing 2 2 2
MIRT673159 C1orf50 chromosome 1 open reading frame 50 2 2
MIRT673272 RUNDC1 RUN domain containing 1 2 2
MIRT673332 THAP1 THAP domain containing 1 2 2
MIRT673351 SLC35F6 solute carrier family 35 member F6 2 2
MIRT673667 ZNF440 zinc finger protein 440 2 2
MIRT673904 DCTN6 dynactin subunit 6 2 2
MIRT674096 PLEKHA1 pleckstrin homology domain containing A1 2 2
MIRT674401 MYCBP MYC binding protein 2 2
MIRT674525 PRR23A proline rich 23A 2 2
MIRT674793 NPR1 natriuretic peptide receptor 1 2 2
MIRT674833 ADAMTS4 ADAM metallopeptidase with thrombospondin type 1 motif 4 2 2
MIRT675066 FGD6 FYVE, RhoGEF and PH domain containing 6 2 2
MIRT675080 CCR6 C-C motif chemokine receptor 6 2 2
MIRT675126 FSD2 fibronectin type III and SPRY domain containing 2 2 2
MIRT679401 IL10RB interleukin 10 receptor subunit beta 2 2
MIRT689229 RPS19 ribosomal protein S19 2 2
MIRT694008 PPIL4 peptidylprolyl isomerase like 4 2 2
MIRT699671 SFT2D2 SFT2 domain containing 2 2 2
MIRT706213 ACOT9 acyl-CoA thioesterase 9 2 2
MIRT706548 GJD2 gap junction protein delta 2 2 2
MIRT707418 RRP7A ribosomal RNA processing 7 homolog A 2 2
MIRT710648 GLUL glutamate-ammonia ligase 2 2
MIRT719393 NPCA1 Nasopharyngeal carcinoma 1 2 2
MIRT720166 PNPO pyridoxamine 5'-phosphate oxidase 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-4708-5p Fulvestrant 17756771 NSC719276 approved sensitive High Breast Cancer cell line (MCF-7)
hsa-miR-4708-5p Tamoxifen 2733525 NSC180973 approved resistant High Breast Cancer cell line (MCF-7)
hsa-miR-4708-5p Tripterygium wilfordii Hook F sensitive tissue
hsa-miR-4708-5p Cisplatin 5460033 NSC119875 approved sensitive cell line
hsa-miR-4708-5p Paclitaxel 36314 NSC125973 approved resistant cell line (A2780)
hsa-miR-4708-5p Cisplatin 5460033 NSC119875 approved resistant cell line (A2780)

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