miRTarBase - #MIRT677084


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pre-miRNA Information
pre-miRNA hsa-mir-2355   
Genomic Coordinates chr2: 207109987 - 207110073
Description Homo sapiens miR-2355 stem-loop
Comment None
RNA Secondary Structure
Associated Diseases

Mature miRNA Information
Mature miRNA hsa-miR-2355-5p
Sequence 11| AUCCCCAGAUACAAUGGACAA |31
Evidence Experimental
Experiments SOLiD
Editing Events in miRNAs
Modification Type Position on miR Chromosome DNA Strand Genomic Position (hg38) List of PMIDs Variant details
A-to-I 1 2 - 207110063 29233923 MiREDiBase
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1268296297 4 dbSNP
rs1361046879 5 dbSNP
rs1219551997 6 dbSNP
rs1295756820 12 dbSNP
rs1345273529 15 dbSNP
rs977145706 15 dbSNP
rs1209629984 20 dbSNP
rs759940260 21 dbSNP
Putative Targets

miRNA Expression profile
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol SMIM15   
Synonyms C5orf43
Description small integral membrane protein 15
Transcript NM_001048249   
Expression
Putative miRNA Targets on SMIM15
3'UTR of SMIM15
(miRNA target sites are highlighted)		 
>SMIM15|NM_001048249|3'UTR
   1 AGGACTGAACAGGCTTTGCAACCAGAGGAAAATCATTTGGAAAATTACACAGCTTTGGAAGAATCCACTAAAGTTTCTTC
  81 TTTGGATTTCTTGACAGTATGATTTAGTAAATGAAATTTGACCAAATGGAAGAATCATGTTAGTTCTGACCTCAATACTA
 161 TAGTAACTTTTAGGCGTGGGTGTAGAAGTTTATAGGTTTCTATTGACAGTTATTGTAAATTAGCATTTACTGTGGTACAA
 241 ATTCTTTATAACTGACTTAGTCATTTGCCGCTTAGCAGTTTATATACTGAAATGAAAACATCTTGTGGGGAAAAGTGACT
 321 TTAGATTATGAACTCAATTCAAATGAACTCTATTTAAAATGGGGTCCTATTTTGGACAAAGGAAATTAAGAATGTAAAAG
 401 TCAGAACAGTCTTGAGGTAAAAAGTGTGCTTTGGCTTAAAAGGGATACAGTATATTAATTACATCTTTTATTATTATTGT
 481 TTATTTCTTAGAATCATTTCTGGCTTTCTCAAAACAAAATAATATTAATGAGTACTTCTATTTGCTGCATTTTTCTTATT
 561 ACAGCCTTTGAGACAGCTGGTAATTATAAGTCATTTTCCATTTTTTAAAACATAATTTTATAAAGAATTCTCTTATCTCG
 641 ACTATGTAGAATACCACCTACTGGACAGAACAATTTTTGTACTCACAAACACTGCCATTTTCTTAGAGATGGCTTGAGAG
 721 GAGTAACACTATGGTTTAAAGCTTGCAGTAAAAATGCCAAACACTGTAGTACCTTGGAACCCAGTTTATTCTTGTGCTAA
 801 GCAGAACTGTAAAATAGTTAAAATGTCTTATCAAGTAATTCGCCGATTACAAAGACACCATTTGTTTTTTATTTCATTCT
 881 TTGTTTTAACTCATGTGGTAGTGATATTTAATACTTTCTGATCAAACAGGTTCAAAGTAAAACGTTAAATTTCACATTTC
 961 TTTTAAAGAACTCTTAAAGTGTAACAGTTACGCCATACTTCATAAGTGGTAAAGAAAGGTATAAAATTTGGAAACATTTT
1041 GTTGGGCATAGTAGTGATTGGGTGAAAAGGATAAATTATATCAAAATGAGAATGTGCTGTAATTGGAAGTAGGGAGCTAA
1121 AGGATGTTTCTTTCAGTTTAGTAGAACTGGAACGTTTTACTATTAAACATGGCTTTTATAAATGCATGGTCCAATAATTT
1201 TATTCACTGTTAGTATTTAATTCACTGTCAGCTTATTAATGTTTTCTGTACCCATTAATGAATTTTAAATTACAAAAAAT
1281 TGTCTAGCAGCTACAGTTTAAAAATGAAACTAGACATTAAAATAAATTTGATAATTTTTTATAAAGAGGTCCTGGATTTT
1361 TTTTTTTTTTTTGGAGGAACTAACATTTTAAGTTACATTATGTTATGCAAGTTAGAATTTCAGTTACAGCTAAGCATACT
1441 TGTAAAATTTCAGGACTGTATCTCTCAGAACCTAAGGACCACTGAGAATGTTTATGGACCAGTTTGAATAACACTTAATG
1521 CTATTCTTTTAGATTATCAGTCTCTACTTGGTGCCAGTGTGAGCAACACATTATAACTCAAGGCACTGAAATTATTCAGT
1601 GTTAAGATATATCTATAATATGTTTGGTTTTCTGCAATCAAGCTGTTCATGGAAAGAATTTATGTACATGTTTTACTTAG
1681 GGTCACATTTTAGAGTAAAAGTATTGAAGCAAAGGATATAGTCATAATTCTCAGTTACCTACTAAAGTTATGGTTTCATC
1761 AGTTCTGTTGTAACAACTTAAAGGTATTTAAAGGTCTGTTTTTCTATAATTTATTAGTCTTAACACATGCCAGTTTTGTA
1841 TTTGTTTGACATATGCTTTTTTTTTCCACTTTTTTCCATCATTTAGAGAGCCTTTCTATATATGTGAGATTCATACTTTA
1921 CAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAAGGGGCTGGAGTATAGAGAATATTGTGAGGTTTGGGATTCTTC
2001 AGAAAAATCACCTCTCACTGCAACAGAGTACTGTTGTTAAAGAATCCCATTTGCTATTATCTAGAATACTTTTAAGAAAT
2081 TGTTTACTTATTACATTGTTTCTGAAGTTCTGAGAACTAAGTACAATCAATTACCATTCTAAAGCTGGTCTTGGGGGAGA
2161 AAGAAAGCTGTCCCCTGGCTGCCTCTAACAAACATTTTATCAAAAATCAGCAAATAAATAAAAGTTTGTATGTCAGAAAA
2241 AAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' aacagguaacauagACCCCUa 5'
                        |||||| 
Target 5' atgaaaacatcttgTGGGGAa 3'
 292 - 312 120.00 -10.20
2 
miRNA  3' aacaGGUAACAU-------A-GACCCCUa 5'
              ||||| ||       | :|||||: 
Target 5' attaCCATTCTAAAGCTGGTCTTGGGGGa 3'
 2130 - 2158 117.00 -13.70
3 
miRNA  3' aacAGGUAACAUAGACCCCua 5'
             ||:|||  |  |||||  
Target 5' aacTCTATTTAAAATGGGGtc 3'
 346 - 366 110.00 -11.80
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30177330 127 COSMIC
COSN31541397 132 COSMIC
COSN31586852 270 COSMIC
COSN24301137 501 COSMIC
COSN31585875 570 COSMIC
COSN31609475 739 COSMIC
COSN7949053 857 COSMIC
COSN15567127 1207 COSMIC
COSN2113075 1268 COSMIC
COSN20101291 1357 COSMIC
COSN20101293 1357 COSMIC
COSN2113074 1664 COSMIC
COSN24448370 1798 COSMIC
COSN7949052 1865 COSMIC
COSN20101289 1922 COSMIC
COSN23382453 1955 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs767657454 2 dbSNP
rs868270757 10 dbSNP
rs1383809861 12 dbSNP
rs774011591 13 dbSNP
rs971029416 14 dbSNP
rs373054353 24 dbSNP
rs1298828282 25 dbSNP
rs764037305 26 dbSNP
rs762926038 31 dbSNP
rs374184400 34 dbSNP
rs769385562 42 dbSNP
rs745388126 45 dbSNP
rs757003437 47 dbSNP
rs776251109 51 dbSNP
rs540511021 65 dbSNP
rs148875191 70 dbSNP
rs1438663415 78 dbSNP
rs1378061787 84 dbSNP
rs1386549075 85 dbSNP
rs769829833 91 dbSNP
rs1427185107 95 dbSNP
rs1165914345 103 dbSNP
rs1163212427 107 dbSNP
rs1473320379 108 dbSNP
rs958614611 129 dbSNP
rs1034105968 134 dbSNP
rs1237481778 138 dbSNP
rs752432553 146 dbSNP
rs1375014733 148 dbSNP
rs1446303547 153 dbSNP
rs1315904406 161 dbSNP
rs1384997181 161 dbSNP
rs1226549550 163 dbSNP
rs1288994562 175 dbSNP
rs557946420 176 dbSNP
rs1263939004 190 dbSNP
rs1216205658 192 dbSNP
rs1457087894 196 dbSNP
rs1351835786 206 dbSNP
rs1183821769 225 dbSNP
rs963223886 230 dbSNP
rs1294193928 234 dbSNP
rs183462815 236 dbSNP
rs1413751545 237 dbSNP
rs1356703089 239 dbSNP
rs1173057713 240 dbSNP
rs1398549804 257 dbSNP
rs1004704939 258 dbSNP
rs1283540577 268 dbSNP
rs890412056 269 dbSNP
rs372931608 270 dbSNP
rs1298320368 273 dbSNP
rs1362635524 293 dbSNP
rs1216242950 294 dbSNP
rs1276146586 300 dbSNP
rs1317005270 301 dbSNP
rs542690975 305 dbSNP
rs1358294102 307 dbSNP
rs1440305677 319 dbSNP
rs1208873560 321 dbSNP
rs575375888 329 dbSNP
rs1476063806 331 dbSNP
rs370908884 340 dbSNP
rs771584172 341 dbSNP
rs1413458032 349 dbSNP
rs1053812842 351 dbSNP
rs936735179 361 dbSNP
rs1177457095 362 dbSNP
rs924010618 364 dbSNP
rs754626965 374 dbSNP
rs1476652676 392 dbSNP
rs1042565410 402 dbSNP
rs751143919 404 dbSNP
rs918216490 405 dbSNP
rs991443144 405 dbSNP
rs556238411 434 dbSNP
rs563892796 435 dbSNP
rs1193102875 446 dbSNP
rs1223858040 447 dbSNP
rs567594273 448 dbSNP
rs958410783 463 dbSNP
rs1487342259 470 dbSNP
rs1342509480 473 dbSNP
rs1284347072 479 dbSNP
rs148341488 479 dbSNP
rs1448163530 482 dbSNP
rs1219461025 483 dbSNP
rs1222511426 487 dbSNP
rs1261603970 488 dbSNP
rs1451987602 490 dbSNP
rs973044955 519 dbSNP
rs1226365758 520 dbSNP
rs1477201701 520 dbSNP
rs1172148094 523 dbSNP
rs963152809 523 dbSNP
rs1319975292 524 dbSNP
rs1278745805 527 dbSNP
rs1156812784 530 dbSNP
rs1217551010 530 dbSNP
rs1359874570 535 dbSNP
rs1405467335 540 dbSNP
rs1304796367 547 dbSNP
rs774187769 553 dbSNP
rs1329068595 558 dbSNP
rs1435323951 567 dbSNP
rs1337685484 570 dbSNP
rs1284825810 580 dbSNP
rs1014674160 583 dbSNP
rs1237372622 584 dbSNP
rs1284661161 601 dbSNP
rs1436693420 609 dbSNP
rs1349698796 615 dbSNP
rs1353695535 639 dbSNP
rs1322034830 640 dbSNP
rs368037794 645 dbSNP
rs1269920569 658 dbSNP
rs1411981406 669 dbSNP
rs1450178681 671 dbSNP
rs192242164 679 dbSNP
rs544893518 679 dbSNP
rs996469452 686 dbSNP
rs534244797 687 dbSNP
rs571814252 704 dbSNP
rs1170587525 705 dbSNP
rs1388336738 706 dbSNP
rs749157246 717 dbSNP
rs1404506111 719 dbSNP
rs753312568 720 dbSNP
rs900529519 731 dbSNP
rs1444109777 745 dbSNP
rs1053739104 746 dbSNP
rs1247369153 748 dbSNP
rs1267708600 756 dbSNP
rs1354727325 757 dbSNP
rs1209665476 760 dbSNP
rs1274131306 766 dbSNP
rs1431478094 766 dbSNP
rs1459310097 768 dbSNP
rs1192024627 772 dbSNP
rs1237651791 780 dbSNP
rs1445261107 784 dbSNP
rs549433993 815 dbSNP
rs762997000 821 dbSNP
rs1366685726 833 dbSNP
rs1245176185 836 dbSNP
rs1000810771 841 dbSNP
rs902642837 842 dbSNP
rs72759240 844 dbSNP
rs949584330 845 dbSNP
rs1197874113 851 dbSNP
rs918048367 856 dbSNP
rs1302715956 859 dbSNP
rs1055397270 861 dbSNP
rs938284775 864 dbSNP
rs551961107 871 dbSNP
rs920159829 871 dbSNP
rs963036952 872 dbSNP
rs531765423 877 dbSNP
rs1444899914 878 dbSNP
rs1202297191 887 dbSNP
rs907626363 888 dbSNP
rs1254155339 895 dbSNP
rs1423242344 897 dbSNP
rs1193731322 908 dbSNP
rs983571203 922 dbSNP
rs1378034527 927 dbSNP
rs1169561866 928 dbSNP
rs765146867 929 dbSNP
rs1467245275 937 dbSNP
rs74917535 943 dbSNP
rs530880532 944 dbSNP
rs1408897921 945 dbSNP
rs974584565 949 dbSNP
rs1413962625 959 dbSNP
rs1342808057 965 dbSNP
rs1217226830 968 dbSNP
rs755969211 971 dbSNP
rs180768088 974 dbSNP
rs1179098540 976 dbSNP
rs1324610912 979 dbSNP
rs1235693459 983 dbSNP
rs1032214726 991 dbSNP
rs1482935506 992 dbSNP
rs1000862998 1002 dbSNP
rs1265199798 1006 dbSNP
rs144862587 1009 dbSNP
rs1192738227 1019 dbSNP
rs1434543828 1030 dbSNP
rs540198600 1038 dbSNP
rs1173441635 1041 dbSNP
rs1177973452 1042 dbSNP
rs1376594983 1043 dbSNP
rs1453799426 1051 dbSNP
rs775831887 1058 dbSNP
rs1363057146 1078 dbSNP
rs1021049991 1082 dbSNP
rs1014062361 1093 dbSNP
rs1368666913 1100 dbSNP
rs1257669934 1103 dbSNP
rs1210284032 1109 dbSNP
rs1299407455 1110 dbSNP
rs1231498886 1114 dbSNP
rs896547811 1118 dbSNP
rs1241019092 1130 dbSNP
rs1055727810 1139 dbSNP
rs140536797 1142 dbSNP
rs76253290 1148 dbSNP
rs1215718581 1153 dbSNP
rs1241805012 1154 dbSNP
rs898787490 1166 dbSNP
rs1038591429 1168 dbSNP
rs1266280931 1170 dbSNP
rs1419278985 1181 dbSNP
rs608487 1190 dbSNP
rs941684110 1192 dbSNP
rs907510026 1205 dbSNP
rs1043204 1206 dbSNP
rs755746630 1223 dbSNP
rs1444997007 1232 dbSNP
rs1382035648 1252 dbSNP
rs1303927551 1259 dbSNP
rs983240595 1260 dbSNP
rs1350858429 1261 dbSNP
rs933089388 1269 dbSNP
rs1367233380 1273 dbSNP
rs189861262 1284 dbSNP
rs544439619 1289 dbSNP
rs975017718 1293 dbSNP
rs1374681021 1300 dbSNP
rs1161587232 1309 dbSNP
rs1420500653 1316 dbSNP
rs1239360116 1329 dbSNP
rs1290452506 1332 dbSNP
rs964514482 1336 dbSNP
rs1302241624 1341 dbSNP
rs1379886506 1346 dbSNP
rs1157881442 1351 dbSNP
rs1437578063 1355 dbSNP
rs377439551 1357 dbSNP
rs33918253 1359 dbSNP
rs1258260626 1360 dbSNP
rs1182311215 1369 dbSNP
rs75388059 1371 dbSNP
rs201591595 1372 dbSNP
rs1426053637 1373 dbSNP
rs147178654 1373 dbSNP
rs1480792837 1373 dbSNP
rs1491413475 1373 dbSNP
rs398064853 1373 dbSNP
rs74270782 1373 dbSNP
rs747028434 1373 dbSNP
rs749990011 1373 dbSNP
rs776669369 1373 dbSNP
rs796985974 1373 dbSNP
rs869175256 1373 dbSNP
rs1491133847 1374 dbSNP
rs75150417 1374 dbSNP
rs1349664115 1375 dbSNP
rs573292978 1376 dbSNP
rs1333551202 1384 dbSNP
rs1398237264 1391 dbSNP
rs1446180996 1396 dbSNP
rs1482655726 1406 dbSNP
rs1235200530 1410 dbSNP
rs1381320647 1423 dbSNP
rs1226530031 1428 dbSNP
rs1311747660 1436 dbSNP
rs1218124561 1440 dbSNP
rs1200166367 1447 dbSNP
rs1255719738 1448 dbSNP
rs1354031134 1450 dbSNP
rs1210273146 1458 dbSNP
rs1238690718 1472 dbSNP
rs1284846163 1473 dbSNP
rs1238509966 1476 dbSNP
rs1323806569 1479 dbSNP
rs1478327873 1480 dbSNP
rs1032224438 1481 dbSNP
rs1314078930 1496 dbSNP
rs979286611 1499 dbSNP
rs966698232 1504 dbSNP
rs1377114850 1506 dbSNP
rs1300161847 1508 dbSNP
rs1400671019 1523 dbSNP
rs1302143801 1531 dbSNP
rs1021396094 1535 dbSNP
rs1220284364 1539 dbSNP
rs1296579862 1544 dbSNP
rs1417638374 1557 dbSNP
rs868780256 1565 dbSNP
rs1316987484 1571 dbSNP
rs774423135 1573 dbSNP
rs1280137654 1579 dbSNP
rs1309879726 1588 dbSNP
rs1208678135 1592 dbSNP
rs1013572439 1598 dbSNP
rs1488889648 1621 dbSNP
rs1165435047 1622 dbSNP
rs1196366115 1627 dbSNP
rs1261472214 1641 dbSNP
rs1423214110 1642 dbSNP
rs896595755 1645 dbSNP
rs1173319775 1651 dbSNP
rs1412795770 1656 dbSNP
rs1427382212 1656 dbSNP
rs1175412337 1660 dbSNP
rs1361147222 1666 dbSNP
rs1401754677 1667 dbSNP
rs1296189276 1683 dbSNP
rs1339530748 1690 dbSNP
rs1411502607 1700 dbSNP
rs1436772365 1704 dbSNP
rs1276206934 1707 dbSNP
rs1365009125 1711 dbSNP
rs575339070 1713 dbSNP
rs1218179091 1714 dbSNP
rs1286091624 1715 dbSNP
rs1355129689 1717 dbSNP
rs1195285821 1725 dbSNP
rs562680274 1739 dbSNP
rs1002374732 1743 dbSNP
rs1262022243 1745 dbSNP
rs898630433 1761 dbSNP
rs1213927479 1765 dbSNP
rs1489682625 1782 dbSNP
rs1266366813 1790 dbSNP
rs1156709027 1798 dbSNP
rs1421451274 1798 dbSNP
rs749555040 1798 dbSNP
rs542692658 1806 dbSNP
rs1405777364 1807 dbSNP
rs145523610 1816 dbSNP
rs1329051045 1817 dbSNP
rs1320497225 1826 dbSNP
rs185149570 1827 dbSNP
rs1280638439 1828 dbSNP
rs940232177 1831 dbSNP
rs1339853291 1849 dbSNP
rs553767353 1854 dbSNP
rs1276559659 1861 dbSNP
rs887348929 1865 dbSNP
rs1047880895 1866 dbSNP
rs1213231221 1866 dbSNP
rs1350554102 1866 dbSNP
rs932994818 1866 dbSNP
rs1391682291 1873 dbSNP
rs533902222 1875 dbSNP
rs1441573139 1883 dbSNP
rs1293945072 1890 dbSNP
rs1176818363 1891 dbSNP
rs757432839 1898 dbSNP
rs1457205534 1904 dbSNP
rs1164847640 1913 dbSNP
rs578199604 1916 dbSNP
rs923053920 1919 dbSNP
rs1447371516 1920 dbSNP
rs558157290 1922 dbSNP
rs1172644967 1923 dbSNP
rs56157913 1923 dbSNP
rs974480632 1926 dbSNP
rs776846367 1928 dbSNP
rs538466209 1933 dbSNP
rs1477868469 1937 dbSNP
rs943216846 1943 dbSNP
rs1330066873 1945 dbSNP
rs1335749517 1947 dbSNP
rs1422219806 1949 dbSNP
rs1246161684 1951 dbSNP
rs1191107410 1956 dbSNP
rs1491580858 1956 dbSNP
rs1196889921 1957 dbSNP
rs1232927416 1957 dbSNP
rs1249901696 1957 dbSNP
rs1258987997 1957 dbSNP
rs1286329538 1957 dbSNP
rs1354622888 1957 dbSNP
rs1442937192 1957 dbSNP
rs1459238351 1957 dbSNP
rs1491109180 1957 dbSNP
rs71606670 1957 dbSNP
rs866089082 1957 dbSNP
rs1229755266 1958 dbSNP
rs1434588723 1959 dbSNP
rs1338922475 1960 dbSNP
rs1264976466 1963 dbSNP
rs796071432 1963 dbSNP
rs1191042064 1995 dbSNP
rs925092139 1998 dbSNP
rs1487707806 2000 dbSNP
rs927271280 2009 dbSNP
rs1377743721 2011 dbSNP
rs979214555 2013 dbSNP
rs1467100526 2021 dbSNP
rs1170247966 2025 dbSNP
rs1374190419 2030 dbSNP
rs966626437 2046 dbSNP
rs1299266808 2047 dbSNP
rs1342761460 2049 dbSNP
rs913809290 2059 dbSNP
rs992606946 2073 dbSNP
rs146776774 2076 dbSNP
rs573957882 2102 dbSNP
rs1034188023 2106 dbSNP
rs1199757663 2109 dbSNP
rs1002260159 2110 dbSNP
rs1317400599 2112 dbSNP
rs1256280465 2117 dbSNP
rs1235483958 2130 dbSNP
rs962890039 2133 dbSNP
rs1300841574 2136 dbSNP
rs1185608987 2139 dbSNP
rs796581972 2148 dbSNP
rs116698095 2153 dbSNP
rs1434488554 2157 dbSNP
rs1196142331 2158 dbSNP
rs1004371733 2172 dbSNP
rs887392070 2175 dbSNP
rs56145745 2185 dbSNP
rs1368437562 2189 dbSNP
rs1333305839 2193 dbSNP
rs1452125983 2195 dbSNP
rs1466037281 2209 dbSNP
rs555615577 2239 dbSNP
rs1391739746 2242 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084043. RNA binding protein: AGO2. Condition:CLIP_arsenite_rep2 ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' aacagguaacAUAGACCCCUa 5'
                    || ||||||| 
Target 5' ----------UA-CUGGGGAg 3'
 		1 - 10
Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084043
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_arsenite_rep2
Location of target site ENST00000339020.3 | 3UTR | UACUGGGGAGGCUGAGGCAGGAGAAUUGCUUGAACCCAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
164 hsa-miR-2355-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT190653 PABPN1 poly(A) binding protein nuclear 1 2 2
MIRT286477 TAOK1 TAO kinase 1 2 4
MIRT318993 GIGYF1 GRB10 interacting GYF protein 1 2 2
MIRT345622 DCAF7 DDB1 and CUL4 associated factor 7 2 4
MIRT442513 SYT7 synaptotagmin 7 2 4
MIRT444247 CTNND1 catenin delta 1 2 2
MIRT444780 ECE1 endothelin converting enzyme 1 2 2
MIRT456525 TMEM63A transmembrane protein 63A 2 2
MIRT468960 RPRD2 regulation of nuclear pre-mRNA domain containing 2 2 2
MIRT469834 R3HDM4 R3H domain containing 4 2 2
MIRT470027 PTP4A1 protein tyrosine phosphatase type IVA, member 1 2 2
MIRT471167 PHB2 prohibitin 2 2 2
MIRT475819 HDGF heparin binding growth factor 2 4
MIRT479389 CDKN1B cyclin dependent kinase inhibitor 1B 2 6
MIRT481747 APH1A aph-1 homolog A, gamma-secretase subunit 2 2
MIRT485220 PRICKLE1 prickle planar cell polarity protein 1 2 2
MIRT487259 CCNF cyclin F 2 2
MIRT489971 MCC mutated in colorectal cancers 2 4
MIRT491399 C1D C1D nuclear receptor corepressor 2 2
MIRT494959 CCDC144A coiled-coil domain containing 144A 2 4
MIRT494979 PPP5D1 PPP5 tetratricopeptide repeat domain containing 1 2 2
MIRT495225 CHST14 carbohydrate sulfotransferase 14 2 2
MIRT496550 TBX15 T-box 15 2 2
MIRT501612 PISD phosphatidylserine decarboxylase 2 2
MIRT509393 TRIM24 tripartite motif containing 24 2 2
MIRT519616 ZNF788 zinc finger family member 788 2 4
MIRT519738 ZNF394 zinc finger protein 394 2 4
MIRT521230 SAR1A secretion associated Ras related GTPase 1A 2 4
MIRT521801 POM121C POM121 transmembrane nucleoporin C 2 2
MIRT526233 C2orf15 chromosome 2 open reading frame 15 2 2
MIRT526932 CMSS1 cms1 ribosomal small subunit homolog (yeast) 2 2
MIRT529189 ACBD7 acyl-CoA binding domain containing 7 2 2
MIRT529329 ZNF649 zinc finger protein 649 2 2
MIRT529698 MRPL30 mitochondrial ribosomal protein L30 2 2
MIRT529921 TOP1MT DNA topoisomerase I mitochondrial 2 2
MIRT530032 SMC1A structural maintenance of chromosomes 1A 2 2
MIRT530898 C9orf3 chromosome 9 open reading frame 3 2 2
MIRT531621 TM4SF5 transmembrane 4 L six family member 5 2 4
MIRT533132 XIAP X-linked inhibitor of apoptosis 2 2
MIRT533210 WAPAL WAPL cohesin release factor 2 2
MIRT534058 ST6GALNAC3 ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 3 2 2
MIRT535487 PARVB parvin beta 2 2
MIRT536749 HOXD9 homeobox D9 2 2
MIRT551456 CARKD NAD(P)HX dehydratase 2 2
MIRT551858 ASB6 ankyrin repeat and SOCS box containing 6 2 2
MIRT552095 FAM8A1 family with sequence similarity 8 member A1 2 2
MIRT552332 ZNF704 zinc finger protein 704 2 2
MIRT552549 ZFP36L2 ZFP36 ring finger protein like 2 2 2
MIRT561505 SRSF2 serine and arginine rich splicing factor 2 2 2
MIRT562702 ZNF415 zinc finger protein 415 2 2
MIRT563052 ZNF138 zinc finger protein 138 2 2
MIRT564592 ZNF791 zinc finger protein 791 2 2
MIRT566422 PIGA phosphatidylinositol glycan anchor biosynthesis class A 2 2
MIRT570176 RCBTB1 RCC1 and BTB domain containing protein 1 2 2
MIRT570866 ZNF525 zinc finger protein 525 2 2
MIRT570875 ZFP1 ZFP1 zinc finger protein 2 2
MIRT571517 ZNF625 zinc finger protein 625 2 2
MIRT572551 DKK3 dickkopf WNT signaling pathway inhibitor 3 2 2
MIRT610894 HINFP histone H4 transcription factor 2 2
MIRT611037 PTGES prostaglandin E synthase 2 2
MIRT611479 HSPA4 heat shock protein family A (Hsp70) member 4 2 4
MIRT612419 SP1 Sp1 transcription factor 2 2
MIRT614478 FRMPD3 FERM and PDZ domain containing 3 2 2
MIRT616318 CACNA1A calcium voltage-gated channel subunit alpha1 A 2 2
MIRT616929 ZNF638 zinc finger protein 638 2 2
MIRT618131 GABARAPL3 GABA type A receptor associated protein like 3 pseudogene 2 2
MIRT618452 ZFP30 ZFP30 zinc finger protein 2 4
MIRT620671 BBS5 Bardet-Biedl syndrome 5 2 2
MIRT620713 ASB16 ankyrin repeat and SOCS box containing 16 2 2
MIRT621126 EMC7 ER membrane protein complex subunit 7 2 4
MIRT621443 TOMM22 translocase of outer mitochondrial membrane 22 2 2
MIRT623491 KCTD11 potassium channel tetramerization domain containing 11 2 2
MIRT623783 GOSR1 golgi SNAP receptor complex member 1 2 2
MIRT623918 FNBP1L formin binding protein 1 like 2 2
MIRT625723 CXorf38 chromosome X open reading frame 38 2 2
MIRT625778 GCNT1 glucosaminyl (N-acetyl) transferase 1, core 2 2 2
MIRT626940 TTR transthyretin 2 2
MIRT627357 TSC22D2 TSC22 domain family member 2 2 2
MIRT632078 ALDH1A2 aldehyde dehydrogenase 1 family member A2 2 2
MIRT633361 FAM71F2 family with sequence similarity 71 member F2 2 2
MIRT639793 MVK mevalonate kinase 2 2
MIRT643761 NAGK N-acetylglucosamine kinase 2 2
MIRT644358 NDUFAF6 NADH:ubiquinone oxidoreductase complex assembly factor 6 2 2
MIRT645558 ZMYM1 zinc finger MYM-type containing 1 2 2
MIRT646588 ANKRD36 ankyrin repeat domain 36 2 2
MIRT646631 SLC25A46 solute carrier family 25 member 46 2 2
MIRT648066 HSPA6 heat shock protein family A (Hsp70) member 6 2 2
MIRT648142 SUCLG2 succinate-CoA ligase GDP-forming beta subunit 2 2
MIRT648416 MYOZ3 myozenin 3 2 2
MIRT649218 AMMECR1L AMMECR1 like 2 2
MIRT649772 MRPS27 mitochondrial ribosomal protein S27 2 4
MIRT650510 UFM1 ubiquitin fold modifier 1 2 2
MIRT651007 ZNF740 zinc finger protein 740 2 2
MIRT651524 WNT2B Wnt family member 2B 2 2
MIRT652014 TTYH3 tweety family member 3 2 2
MIRT652513 TMEM109 transmembrane protein 109 2 2
MIRT652686 THUMPD3 THUMP domain containing 3 2 2
MIRT652772 TENM3 teneurin transmembrane protein 3 2 2
MIRT653160 SPRY4 sprouty RTK signaling antagonist 4 2 2
MIRT653765 SIX5 SIX homeobox 5 2 2
MIRT653792 SIRPA signal regulatory protein alpha 2 2
MIRT655649 OAZ2 ornithine decarboxylase antizyme 2 2 2
MIRT656263 MEX3A mex-3 RNA binding family member A 2 2
MIRT657044 KCNH6 potassium voltage-gated channel subfamily H member 6 2 2
MIRT657375 HMGA1 high mobility group AT-hook 1 2 2
MIRT657532 GTDC1 glycosyltransferase like domain containing 1 2 2
MIRT657542 GSTO2 glutathione S-transferase omega 2 2 2
MIRT657828 GJD3 gap junction protein delta 3 2 2
MIRT658946 DOK6 docking protein 6 2 2
MIRT660363 BACH2 BTB domain and CNC homolog 2 2 2
MIRT660406 ATXN7L3 ataxin 7 like 3 2 2
MIRT660948 ABL2 ABL proto-oncogene 2, non-receptor tyrosine kinase 2 2
MIRT661031 AAK1 AP2 associated kinase 1 2 2
MIRT662435 RALGAPA1 Ral GTPase activating protein catalytic alpha subunit 1 2 2
MIRT668419 FAM217B family with sequence similarity 217 member B 2 2
MIRT669332 BSND barttin CLCNK type accessory beta subunit 2 2
MIRT676105 CLUAP1 clusterin associated protein 1 2 2
MIRT677084 SMIM15 small integral membrane protein 15 2 2
MIRT677127 OGFOD3 2-oxoglutarate and iron dependent oxygenase domain containing 3 2 2
MIRT678001 CPM carboxypeptidase M 2 2
MIRT678531 TMEM78 transmembrane protein 78 2 2
MIRT680000 ZNF860 zinc finger protein 860 2 2
MIRT680098 PXMP4 peroxisomal membrane protein 4 2 2
MIRT686795 SOX12 SRY-box 12 2 2
MIRT698887 SPTBN2 spectrin beta, non-erythrocytic 2 2 2
MIRT699371 SLC30A6 solute carrier family 30 member 6 2 2
MIRT702005 MIDN midnolin 2 2
MIRT708101 IGF2BP1 insulin like growth factor 2 mRNA binding protein 1 2 2
MIRT709563 GYG2 glycogenin 2 2 2
MIRT710559 PPP2R5E protein phosphatase 2 regulatory subunit B'epsilon 2 2
MIRT710585 CDCA4 cell division cycle associated 4 2 2
MIRT710873 NT5DC1 5'-nucleotidase domain containing 1 2 2
MIRT711876 VASP vasodilator stimulated phosphoprotein 2 2
MIRT712226 RIMS3 regulating synaptic membrane exocytosis 3 2 2
MIRT713211 FAM13A family with sequence similarity 13 member A 2 2
MIRT713261 GPR55 G protein-coupled receptor 55 2 2
MIRT713327 DNAJC28 DnaJ heat shock protein family (Hsp40) member C28 2 2
MIRT713889 RNF19B ring finger protein 19B 2 2
MIRT714015 UBAP2L ubiquitin associated protein 2 like 2 2
MIRT714202 NLRP12 NLR family pyrin domain containing 12 2 2
MIRT714717 VPS8 VPS8, CORVET complex subunit 2 2
MIRT714748 ZNF385D zinc finger protein 385D 2 2
MIRT714806 ARHGEF19 Rho guanine nucleotide exchange factor 19 2 2
MIRT714852 ADAMTS17 ADAM metallopeptidase with thrombospondin type 1 motif 17 2 2
MIRT715717 MRPL4 mitochondrial ribosomal protein L4 2 2
MIRT715852 GRIN2A glutamate ionotropic receptor NMDA type subunit 2A 2 2
MIRT716853 MFSD12 major facilitator superfamily domain containing 12 2 2
MIRT717134 EBPL emopamil binding protein like 2 2
MIRT717248 TMEM246 transmembrane protein 246 2 2
MIRT717997 ZNF79 zinc finger protein 79 2 2
MIRT718341 PURA purine rich element binding protein A 2 2
MIRT719587 TFCP2L1 transcription factor CP2 like 1 2 2
MIRT720520 PTGR2 prostaglandin reductase 2 2 2
MIRT720671 SLC39A13 solute carrier family 39 member 13 2 2
MIRT721213 DCLK3 doublecortin like kinase 3 2 2
MIRT721757 INF2 inverted formin, FH2 and WH2 domain containing 2 2
MIRT721826 POU6F1 POU class 6 homeobox 1 2 2
MIRT722091 SUSD1 sushi domain containing 1 2 2
MIRT722275 LURAP1 leucine rich adaptor protein 1 2 2
MIRT722802 ARHGAP19 Rho GTPase activating protein 19 2 2
MIRT722866 FAM212B family with sequence similarity 212 member B 2 2
MIRT723113 ZSCAN16 zinc finger and SCAN domain containing 16 2 2
MIRT724378 NEK8 NIMA related kinase 8 2 2
MIRT732775 PYCR1 pyrroline-5-carboxylate reductase 1 3 0
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-2355 Ceritinib 57379345 NSC776422 approved resistant cell line (H3122)
hsa-miR-2355-5p Gemcitabine 60750 NSC613327 approved sensitive High Pancreatic Cancer cell line (PANC-1)
hsa-miR-2355-5p Fluorouracil 3385 NSC19893 approved sensitive High Pancreatic Cancer cell line (PANC-1)
hsa-miR-2355-5p Imatinib 5291 NSC743414 approved resistant High Chronic Myelogenous Leukemia cell line (K562)
hsa-miR-2355-5p Cisplatin 5460033 NSC119875 approved resistant High Non-Small Cell Lung Cancer cell line (A549, HCC827)
hsa-miR-2355-5p Gefitinib 123631 NSC715055 approved resistant High Non-Small Cell Lung Cancer cell line (A549, HCC827)
hsa-miR-2355-5p Temozolomide 5394 NSC362856 approved resistant High Glioma tissue
hsa-miR-2355-5p Osimertinib 71496458 NSC779217 approved sensitive cell line (HCC827)
hsa-miR-2355-5p Vemurafenib 42611257 NSC761431 approved resistant cell line (451Lu)
hsa-miR-2355-5p Cisplatin 5460033 NSC119875 approved resistant cell line (A549)
hsa-miR-2355-5p Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-2355-5p Tamoxifen+Fulvestrant sensitive cell line (LCC9)
hsa-miR-2355-5p Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-2355-5p Ceritinib 57379345 NSC776422 approved resistant cell line (H3122)
hsa-miR-2355-5p Cisplatin 5460033 NSC119875 approved sensitive cell line (TOV-112D)
hsa-miR-2355-5p Cisplatin 5460033 NSC119875 approved sensitive cell line (OVSAHO)

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