pre-miRNA Information | |
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pre-miRNA | hsa-mir-3622a |
Genomic Coordinates | chr8: 27701677 - 27701759 |
Description | Homo sapiens miR-3622a stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Mature miRNA Information | ||||||||||||||||||||||||||||||||||||
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Mature miRNA | hsa-miR-3622a-5p | |||||||||||||||||||||||||||||||||||
Sequence | 14| CAGGCACGGGAGCUCAGGUGAG |35 | |||||||||||||||||||||||||||||||||||
Evidence | Experimental | |||||||||||||||||||||||||||||||||||
Experiments | Illumina | |||||||||||||||||||||||||||||||||||
Editing Events in miRNAs |
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SNPs in miRNA |
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Putative Targets |
miRNA Expression profile | |
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Human miRNA Tissue Atlas | |
miRNAs in Extracellular Vesicles |
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Circulating MicroRNA Expression Profiling |
Gene Information | |||||||||||||||||||||
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Gene Symbol | C9orf64 | ||||||||||||||||||||
Synonyms | - | ||||||||||||||||||||
Description | chromosome 9 open reading frame 64 | ||||||||||||||||||||
Transcript | NM_032307 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on C9orf64 | |||||||||||||||||||||
3'UTR of C9orf64 (miRNA target sites are highlighted) |
>C9orf64|NM_032307|3'UTR 1 CCTCAAGTGTAAACTGATCCAAAGAAAACCCCCTGCGTTTTATATCATATCATCTGTACAGTTTTGCTTTGATATTTAGA 81 GAACATGATCGAGGTTATAGGAAATTGATTGCCCATTCTCACTTGAAAAATACTTCCTAGGCCGGGCACAGTGGTTTATG 161 CCTGTAATCCCAGCACTGTGGGAGGCTGAGGCGGGTGGATCATCTGAGGTCAGGAGTTTGAGACCACCTGGCCAACATGG 241 TGAAACCCCATCTCTACTAAAAATACAAAATTAGCTGGGCGTGGTGGCACGTGCCTGTAATCCCAGTTACTTGGGAGGCT 321 GAGGCAAGAGAATCGCTTGAACTCAGAAGGTGGAGGTTGCAGTGAGGCGAGATTAGGCCATTGCACTCCAGCCTCAGCAA 401 CAAGAGTGAAACTTTGTCTCAAAAAACAACAACAACAACAACAACAACAACAAAACTTCCTAGGCCAGATGTGGTGGCTC 481 ATATGTATAATCTTAGCACTTTGGGAGGCCAAGGCAGGAGGATTGCTTGAGGCGAGGAGTTCAAGACCAGCCTAGGCAAC 561 ATAGGGAGACCCCCATCTCTACAAAAATTTAAAAATTAGCCTAGCATGGTGGCACGCTCTGTAATCCCAAATACGCGGTA 641 GGCTGAGGCTGAGGAGGGTGGATTGCTTGAGCCCAGGGGTTTGAGGCTGCAGTGAGCCACAGTCACGCCACTGCATTCTA 721 TCCTGGGCAACAGATGGAGACCTTGTCTCAAAAAAAAAAAATTCCTGACATCGCTATGTATTCCCAACTTTATCATTTGT 801 CTGCCTGTTTAGTTTTGACTTATGTTTTTTTTTTTTCCCCCCTGTGGACATGTAGTTGACGGAAATCGTGAAGGAACTTT 881 AATATTTTATTTAAATTTCCCAAAACTAATCATGCCTTATGTGACTAATCTTCAGTGATAATATTTCATCTACTGATATA 961 TTTTCTTGAGGTGTGTAATTTTCAGTATACCTTAATCATTTGGTATAAAAAAGAGAGAGGTTTTTGATATATGAATGCTG 1041 TTCTTGTAAAAATCAATCTTGACACTTTATTTTAAACTTTTTATTGGTAATGACAGTGGGTTTTGTACATCATGATTTTC 1121 AATTTAGGATATCTGTCTAATTTGTTTTTTCAGAGTAACTATATTGGAATTCAATAAAAATATTCAAAATTTTTCTTAAA 1201 AAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
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miRNA:Target | ---- | ||||||
Validation Method |
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Conditions | Hela | ||||||
Location of target site | 3'UTR | ||||||
Tools used in this research | TargetScan , miRTarCLIP , Piranha | ||||||
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM1048188. RNA binding protein: AGO2. Condition:Hela_AGO2_CLIP_ptb_knockdown
... - Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al., 2013, Cell. |
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miRNA-target interactions (Provided by authors) |
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Article |
- Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al. - Cell, 2013
The induction of pluripotency or trans-differentiation of one cell type to another can be accomplished with cell-lineage-specific transcription factors. Here, we report that repression of a single RNA binding polypyrimidine-tract-binding (PTB) protein, which occurs during normal brain development via the action of miR-124, is sufficient to induce trans-differentiation of fibroblasts into functional neurons. Besides its traditional role in regulated splicing, we show that PTB has a previously undocumented function in the regulation of microRNA functions, suppressing or enhancing microRNA targeting by competitive binding on target mRNA or altering local RNA secondary structure. A key event during neuronal induction is the relief of PTB-mediated blockage of microRNA action on multiple components of the REST complex, thereby derepressing a large array of neuronal genes, including miR-124 and multiple neuronal-specific transcription factors, in nonneuronal cells. This converts a negative feedback loop to a positive one to elicit cellular reprogramming to the neuronal lineage.
LinkOut: [PMID: 23313552]
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CLIP-seq Support 1 for dataset GSM4903832 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | Human neurons / 124TD_shELAVL3_b |
Location of target site | NM_032307 | 3UTR | CUCUACUAAAAAUACAAAAUUAGCUGGGCGUGGUGGCACGUGCCUGUA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE161238 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset GSM4903833 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | Dermal fibroblasts / CTL_TD_21_a |
Location of target site | NM_032307 | 3UTR | CAACAUGGUGAAACCCCAUCUCUACUAAAAAUACAAAAUUAGCUGGGCGUGGUGGCACGUGCCUGUA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE161239 |
CLIP-seq Viewer | Link |
CLIP-seq Support 3 for dataset GSM4903836 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | Dermal fibroblasts / 124_TD_21_a |
Location of target site | NM_032307 | 3UTR | GGCCAACAUGGUGAAACCCCAUCUCUACUAAAAAUACAAAAUUAGCUGGGCGUGGUGGCACGUGCCUGUA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE161239 |
CLIP-seq Viewer | Link |
CLIP-seq Support 4 for dataset GSM4903837 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | Dermal fibroblasts / 124_TD_21_b |
Location of target site | NM_032307 | 3UTR | GUGAAACCCCAUCUCUACUAAAAAUACAAAAUUAGCUGGGCGUGGUGGCACGUGCCUGUA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE161239 |
CLIP-seq Viewer | Link |
CLIP-seq Support 5 for dataset GSM1048188 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | Hela / Hela_AGO2_CLIP_ptb_knockdown |
Location of target site | ENST00000376344.3 | 3UTR | CAAAAUUAGCUGGGCGUGGUGGCACGUG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23313552 / GSE42701 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
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MiRNA-Target Expression Profile (TCGA) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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61 hsa-miR-3622a-5p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT166678 | ZSWIM6 | zinc finger SWIM-type containing 6 | ![]() |
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2 | 2 | ||||||
MIRT452081 | ATP6V0B | ATPase H+ transporting V0 subunit b | ![]() |
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2 | 2 | ||||||
MIRT457039 | S1PR3 | sphingosine-1-phosphate receptor 3 | ![]() |
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2 | 2 | ||||||
MIRT471975 | NR3C1 | nuclear receptor subfamily 3 group C member 1 | ![]() |
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2 | 2 | ||||||
MIRT474065 | LMNB2 | lamin B2 | ![]() |
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2 | 2 | ||||||
MIRT475862 | H6PD | hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase | ![]() |
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2 | 2 | ||||||
MIRT476191 | GOLGA8A | golgin A8 family member A | ![]() |
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2 | 2 | ||||||
MIRT476521 | GABRB1 | gamma-aminobutyric acid type A receptor beta1 subunit | ![]() |
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2 | 2 | ||||||
MIRT483046 | C15orf52 | chromosome 15 open reading frame 52 | ![]() |
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2 | 4 | ||||||
MIRT495541 | EIF3H | eukaryotic translation initiation factor 3 subunit H | ![]() |
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2 | 2 | ||||||
MIRT495892 | CLOCK | clock circadian regulator | ![]() |
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2 | 2 | ||||||
MIRT495992 | LTBP2 | latent transforming growth factor beta binding protein 2 | ![]() |
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2 | 2 | ||||||
MIRT496001 | EMP1 | epithelial membrane protein 1 | ![]() |
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2 | 2 | ||||||
MIRT496208 | PLEKHG2 | pleckstrin homology and RhoGEF domain containing G2 | ![]() |
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2 | 2 | ||||||
MIRT496427 | ACTRT3 | actin related protein T3 | ![]() |
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2 | 2 | ||||||
MIRT496438 | ZNF704 | zinc finger protein 704 | ![]() |
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2 | 2 | ||||||
MIRT496473 | SDE2 | SDE2 telomere maintenance homolog | ![]() |
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2 | 2 | ||||||
MIRT496555 | TBX15 | T-box 15 | ![]() |
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2 | 2 | ||||||
MIRT497202 | CECR1 | adenosine deaminase 2 | ![]() |
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2 | 2 | ||||||
MIRT507632 | CREBZF | CREB/ATF bZIP transcription factor | ![]() |
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2 | 2 | ||||||
MIRT513377 | MGAT4A | mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A | ![]() |
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2 | 2 | ||||||
MIRT523460 | GOLGA8J | golgin A8 family member J | ![]() |
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2 | 2 | ||||||
MIRT523465 | GOLGA8I | golgin A8 family member I, pseudogene | ![]() |
1 | 1 | |||||||
MIRT526049 | GMDS | GDP-mannose 4,6-dehydratase | ![]() |
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2 | 2 | ||||||
MIRT533122 | YIPF4 | Yip1 domain family member 4 | ![]() |
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2 | 2 | ||||||
MIRT534183 | SLC8A1 | solute carrier family 8 member A1 | ![]() |
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2 | 2 | ||||||
MIRT556618 | LCOR | ligand dependent nuclear receptor corepressor | ![]() |
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2 | 2 | ||||||
MIRT563361 | WHSC1 | nuclear receptor binding SET domain protein 2 | ![]() |
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2 | 2 | ||||||
MIRT563868 | FAM206A | family with sequence similarity 206 member A | ![]() |
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2 | 2 | ||||||
MIRT564360 | PPWD1 | peptidylprolyl isomerase domain and WD repeat containing 1 | ![]() |
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2 | 2 | ||||||
MIRT564661 | ZNF449 | zinc finger protein 449 | ![]() |
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2 | 2 | ||||||
MIRT564805 | ZBTB33 | zinc finger and BTB domain containing 33 | ![]() |
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2 | 2 | ||||||
MIRT564867 | ZBED3 | zinc finger BED-type containing 3 | ![]() |
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2 | 2 | ||||||
MIRT565340 | TMEM104 | transmembrane protein 104 | ![]() |
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2 | 2 | ||||||
MIRT566439 | PHF16 | jade family PHD finger 3 | ![]() |
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2 | 2 | ||||||
MIRT567587 | FCHSD2 | FCH and double SH3 domains 2 | ![]() |
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2 | 2 | ||||||
MIRT569597 | C3orf62 | chromosome 3 open reading frame 62 | ![]() |
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2 | 2 | ||||||
MIRT569700 | FMNL3 | formin like 3 | ![]() |
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2 | 2 | ||||||
MIRT575957 | Nanos1 | nanos homolog 1 (Drosophila) | ![]() |
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2 | 3 | ||||||
MIRT576516 | Slc35e2 | solute carrier family 35, member E2 | ![]() |
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2 | 2 | ||||||
MIRT608480 | RRP36 | ribosomal RNA processing 36 | ![]() |
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2 | 2 | ||||||
MIRT614335 | NANOS1 | nanos C2HC-type zinc finger 1 | ![]() |
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2 | 3 | ||||||
MIRT630816 | ATAT1 | alpha tubulin acetyltransferase 1 | ![]() |
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2 | 4 | ||||||
MIRT631708 | C1QTNF6 | C1q and TNF related 6 | ![]() |
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2 | 2 | ||||||
MIRT632369 | SRRD | SRR1 domain containing | ![]() |
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2 | 2 | ||||||
MIRT633471 | DSN1 | DSN1 homolog, MIS12 kinetochore complex component | ![]() |
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2 | 2 | ||||||
MIRT634478 | PAFAH1B2 | platelet activating factor acetylhydrolase 1b catalytic subunit 2 | ![]() |
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2 | 2 | ||||||
MIRT667336 | MTHFD1L | methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like | ![]() |
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2 | 2 | ||||||
MIRT667654 | LFNG | LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase | ![]() |
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2 | 2 | ||||||
MIRT671046 | SS18 | SS18, nBAF chromatin remodeling complex subunit | ![]() |
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2 | 2 | ||||||
MIRT672823 | VEZT | vezatin, adherens junctions transmembrane protein | ![]() |
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2 | 2 | ||||||
MIRT673049 | SGPL1 | sphingosine-1-phosphate lyase 1 | ![]() |
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2 | 2 | ||||||
MIRT673435 | APAF1 | apoptotic peptidase activating factor 1 | ![]() |
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2 | 2 | ||||||
MIRT676096 | DPP9 | dipeptidyl peptidase 9 | ![]() |
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2 | 2 | ||||||
MIRT678059 | RPL7L1 | ribosomal protein L7 like 1 | ![]() |
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2 | 2 | ||||||
MIRT679783 | GOLGA2 | golgin A2 | ![]() |
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2 | 2 | ||||||
MIRT684219 | C9orf64 | chromosome 9 open reading frame 64 | ![]() |
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2 | 2 | ||||||
MIRT697642 | WRN | Werner syndrome RecQ like helicase | ![]() |
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2 | 2 | ||||||
MIRT706595 | C1RL | complement C1r subcomponent like | ![]() |
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2 | 2 | ||||||
MIRT706603 | CCS | copper chaperone for superoxide dismutase | ![]() |
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2 | 2 | ||||||
MIRT717647 | HLX | H2.0 like homeobox | ![]() |
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2 | 2 |
miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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