pre-miRNA Information | |
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pre-miRNA | hsa-mir-4749 |
Genomic Coordinates | chr19: 49854591 - 49854651 |
Description | Homo sapiens miR-4749 stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Mature miRNA Information | |||||||||||||||||||||||||||||||
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Mature miRNA | hsa-miR-4749-3p | ||||||||||||||||||||||||||||||
Sequence | 42| CGCCCCUCCUGCCCCCACAG |61 | ||||||||||||||||||||||||||||||
Evidence | Experimental | ||||||||||||||||||||||||||||||
Experiments | Illumina | DRVs in miRNA |
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SNPs in miRNA |
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Putative Targets |
miRNA Expression profile | |
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Human miRNA Tissue Atlas | |
Circulating MicroRNA Expression Profiling |
Gene Information | ||||||||||||||||
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Gene Symbol | RPL35 | |||||||||||||||
Synonyms | L35 | |||||||||||||||
Description | ribosomal protein L35 | |||||||||||||||
Transcript | NM_007209 | |||||||||||||||
Expression | ||||||||||||||||
Putative miRNA Targets on RPL35 | ||||||||||||||||
3'UTR of RPL35 (miRNA target sites are highlighted) |
>RPL35|NM_007209|3'UTR 1 GGGGCGCATTGTCAATAAAGCACAGCTGGCTGAGACTGCAAAAAAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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DRVs in gene 3'UTRs | ||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | Hela |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM1048188. RNA binding protein: AGO2. Condition:Hela_AGO2_CLIP_ptb_knockdown
... - Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al., 2013, Cell. |
Article |
- Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al. - Cell, 2013
The induction of pluripotency or trans-differentiation of one cell type to another can be accomplished with cell-lineage-specific transcription factors. Here, we report that repression of a single RNA binding polypyrimidine-tract-binding (PTB) protein, which occurs during normal brain development via the action of miR-124, is sufficient to induce trans-differentiation of fibroblasts into functional neurons. Besides its traditional role in regulated splicing, we show that PTB has a previously undocumented function in the regulation of microRNA functions, suppressing or enhancing microRNA targeting by competitive binding on target mRNA or altering local RNA secondary structure. A key event during neuronal induction is the relief of PTB-mediated blockage of microRNA action on multiple components of the REST complex, thereby derepressing a large array of neuronal genes, including miR-124 and multiple neuronal-specific transcription factors, in nonneuronal cells. This converts a negative feedback loop to a positive one to elicit cellular reprogramming to the neuronal lineage.
LinkOut: [PMID: 23313552]
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CLIP-seq Support 1 for dataset GSM1048188 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | Hela / Hela_AGO2_CLIP_ptb_knockdown |
Location of target site | ENST00000373570.4 | 3UTR | cuguacccgcugcggaaguacgcggucaaggccugaGGGGCGCAUUGUCAAUAAAGCACAGCUGGCUGAGA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23313552 / GSE42701 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
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MiRNA-Target Expression Profile (TCGA) | |||||||
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63 hsa-miR-4749-3p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT082256 | MED29 | mediator complex subunit 29 | ![]() |
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2 | 4 | ||||||
MIRT112161 | OTUD3 | OTU deubiquitinase 3 | ![]() |
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2 | 2 | ||||||
MIRT150036 | MIDN | midnolin | ![]() |
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2 | 2 | ||||||
MIRT246308 | HIST2H2AA3 | histone cluster 2 H2A family member a3 | ![]() |
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2 | 4 | ||||||
MIRT246320 | HIST2H2AA4 | histone cluster 2 H2A family member a4 | ![]() |
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2 | 4 | ||||||
MIRT248254 | SP1 | Sp1 transcription factor | ![]() |
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2 | 2 | ||||||
MIRT257944 | GIGYF1 | GRB10 interacting GYF protein 1 | ![]() |
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2 | 2 | ||||||
MIRT466973 | STARD7 | StAR related lipid transfer domain containing 7 | ![]() |
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2 | 4 | ||||||
MIRT492322 | SETD1B | SET domain containing 1B | ![]() |
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2 | 2 | ||||||
MIRT496204 | EFCAB1 | EF-hand calcium binding domain 1 | ![]() |
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2 | 2 | ||||||
MIRT497568 | CCR6 | C-C motif chemokine receptor 6 | ![]() |
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2 | 2 | ||||||
MIRT502990 | CCDC71L | coiled-coil domain containing 71 like | ![]() |
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2 | 8 | ||||||
MIRT508300 | SIX5 | SIX homeobox 5 | ![]() |
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2 | 4 | ||||||
MIRT522472 | ZAK | mitogen-activated protein kinase kinase kinase 20 | ![]() |
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2 | 2 | ||||||
MIRT525825 | VIMP | selenoprotein S | ![]() |
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2 | 4 | ||||||
MIRT528154 | BCL2L1 | BCL2 like 1 | ![]() |
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2 | 2 | ||||||
MIRT532606 | SPTLC2 | serine palmitoyltransferase long chain base subunit 2 | ![]() |
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2 | 2 | ||||||
MIRT551302 | RPRM | reprimo, TP53 dependent G2 arrest mediator homolog | ![]() |
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2 | 2 | ||||||
MIRT568777 | FAM53C | family with sequence similarity 53 member C | ![]() |
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2 | 6 | ||||||
MIRT570896 | METTL21A | methyltransferase like 21A | ![]() |
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2 | 2 | ||||||
MIRT570963 | TMBIM4 | transmembrane BAX inhibitor motif containing 4 | ![]() |
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2 | 2 | ||||||
MIRT571167 | ZNF85 | zinc finger protein 85 | ![]() |
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2 | 2 | ||||||
MIRT576751 | Tmem127 | transmembrane protein 127 | ![]() |
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2 | 2 | ||||||
MIRT609854 | DAZAP2 | DAZ associated protein 2 | ![]() |
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2 | 2 | ||||||
MIRT627101 | PDRG1 | p53 and DNA damage regulated 1 | ![]() |
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2 | 2 | ||||||
MIRT637060 | PRKAG1 | protein kinase AMP-activated non-catalytic subunit gamma 1 | ![]() |
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2 | 2 | ||||||
MIRT639423 | PKP1 | plakophilin 1 | ![]() |
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2 | 2 | ||||||
MIRT643208 | TYW3 | tRNA-yW synthesizing protein 3 homolog | ![]() |
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2 | 4 | ||||||
MIRT646251 | PRSS38 | protease, serine 38 | ![]() |
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2 | 2 | ||||||
MIRT647145 | CYP27C1 | cytochrome P450 family 27 subfamily C member 1 | ![]() |
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2 | 2 | ||||||
MIRT647429 | ZKSCAN2 | zinc finger with KRAB and SCAN domains 2 | ![]() |
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2 | 2 | ||||||
MIRT650666 | GAPDHP44 | glyceraldehyde 3 phosphate dehydrogenase pseudogene 44 | ![]() |
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2 | 2 | ||||||
MIRT651816 | USP49 | ubiquitin specific peptidase 49 | ![]() |
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2 | 2 | ||||||
MIRT657376 | HMGA1 | high mobility group AT-hook 1 | ![]() |
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2 | 2 | ||||||
MIRT658105 | FOXK1 | forkhead box K1 | ![]() |
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2 | 2 | ||||||
MIRT658161 | FCHSD1 | FCH and double SH3 domains 1 | ![]() |
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2 | 2 | ||||||
MIRT662754 | LRRC3C | leucine rich repeat containing 3C | ![]() |
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2 | 2 | ||||||
MIRT667209 | NIPAL1 | NIPA like domain containing 1 | ![]() |
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2 | 2 | ||||||
MIRT687003 | RPL35 | ribosomal protein L35 | ![]() |
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2 | 2 | ||||||
MIRT707057 | NACC2 | NACC family member 2 | ![]() |
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2 | 2 | ||||||
MIRT709048 | MRO | maestro | ![]() |
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2 | 2 | ||||||
MIRT709054 | MGAT5B | mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B | ![]() |
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2 | 2 | ||||||
MIRT709446 | VWA2 | von Willebrand factor A domain containing 2 | ![]() |
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2 | 2 | ||||||
MIRT709847 | SNX12 | sorting nexin 12 | ![]() |
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2 | 2 | ||||||
MIRT710790 | IFNLR1 | interferon lambda receptor 1 | ![]() |
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2 | 2 | ||||||
MIRT711696 | GMPR | guanosine monophosphate reductase | ![]() |
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2 | 2 | ||||||
MIRT712462 | KCNC3 | potassium voltage-gated channel subfamily C member 3 | ![]() |
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2 | 2 | ||||||
MIRT713884 | MOB3A | MOB kinase activator 3A | ![]() |
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2 | 2 | ||||||
MIRT715329 | NTN1 | netrin 1 | ![]() |
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2 | 2 | ||||||
MIRT715927 | CHD4 | chromodomain helicase DNA binding protein 4 | ![]() |
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2 | 2 | ||||||
MIRT716533 | ATF5 | activating transcription factor 5 | ![]() |
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2 | 2 | ||||||
MIRT716937 | CACNB1 | calcium voltage-gated channel auxiliary subunit beta 1 | ![]() |
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2 | 2 | ||||||
MIRT717007 | MFSD6 | major facilitator superfamily domain containing 6 | ![]() |
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2 | 2 | ||||||
MIRT719139 | DPYSL5 | dihydropyrimidinase like 5 | ![]() |
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2 | 2 | ||||||
MIRT720853 | MEF2D | myocyte enhancer factor 2D | ![]() |
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2 | 2 | ||||||
MIRT721484 | LTB4R2 | leukotriene B4 receptor 2 | ![]() |
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2 | 2 | ||||||
MIRT721517 | DKK3 | dickkopf WNT signaling pathway inhibitor 3 | ![]() |
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2 | 2 | ||||||
MIRT722895 | LRRC20 | leucine rich repeat containing 20 | ![]() |
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2 | 2 | ||||||
MIRT723066 | GGA1 | golgi associated, gamma adaptin ear containing, ARF binding protein 1 | ![]() |
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2 | 2 | ||||||
MIRT723086 | INSIG1 | insulin induced gene 1 | ![]() |
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2 | 2 | ||||||
MIRT723353 | ASCL2 | achaete-scute family bHLH transcription factor 2 | ![]() |
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2 | 2 | ||||||
MIRT723483 | MINOS1 | mitochondrial inner membrane organizing system 1 | ![]() |
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2 | 2 | ||||||
MIRT724576 | NOTCH2 | notch 2 | ![]() |
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2 | 2 |
miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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