miRTarBase - #MIRT687146


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pre-miRNA Information
pre-miRNA hsa-mir-4301   
Genomic Coordinates chr11: 113450023 - 113450088
Description Homo sapiens miR-4301 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4301
Sequence 11| UCCCACUACUUCACUUGUGA |30
Evidence Experimental
Experiments SOLiD
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs184176277 2 dbSNP
rs1191324913 13 dbSNP
rs1369198005 15 dbSNP
rs1036973189 16 dbSNP
rs944900541 18 dbSNP
Putative Targets

miRNA Expression profile
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol PTPN12   
Synonyms PTP-PEST, PTPG1
Description protein tyrosine phosphatase, non-receptor type 12
Transcript NM_001131008   
Other Transcripts NM_001131009 , NM_002835   
Expression
Putative miRNA Targets on PTPN12
3'UTR of PTPN12
(miRNA target sites are highlighted)		 
>PTPN12|NM_001131008|3'UTR
   1 TTCAGGGAGCTAGAAGACACTTTAAGTTATACTGGAAAATTCAGGTGCCACTGAAAGCCAGATTTATAGTATTCCATCTT
  81 TAATATGTGGGACTAACAGCAGTGTAGATTGTTACCTTAATATTTTTTGCTGGGACCATCTACCTGCCTTATACTACACT
 161 TAGGAAAAAGTATTACATATGGTTTATTTTGAAACTTCAAGTATTATTGCCTTAATGTCTCTTAACCCTGTTACACGCTG
 241 CTTGTAGACATGTTAATATAGTAATACCTTTATGATATATTGAGTTTAAGGACTACTCTTTTTCTGTTTTATCATGTATG
 321 CATTATTTTGTATATGTACAGGGCAAGTAGGTATATAATTTGATAAAGTTGCAATTGAAATATTATTAACAGAAGATGTA
 401 AGAAATTTCTGCATGGTCTAAATCTTTGTGTACTTTATTTGTAAATTATTTGCCCTGGAGTTTTAGAAAATAGTTTCTGA
 481 ATTTTAAACTTGCTGGATTCATGCAGCCAGCTTTGCAGGTTATCAGAGATCAAAGATTGTAATAATAATTTTGTAAATTG
 561 TAAGCAAAAAGTTATTTTTATATTATATACAGTCTAATTGTTCATCCTAATTGTTCCTGTTTTCATCTAGTCAGAGATTC
 641 AGTAAGTGCCTTGGAACAATATTGAATTCTCTTAGCTTGTGTGTGTTTCTTTAATATTTGAACTCAAGTGGGATTAGAAG
 721 ACTATCAAAATACATGTATGTTTCAGGATATTTGACCTGTCATTAAAAAAAACAAACAGTTTTACAGTGCCAAAAAAAAA
 801 AAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' agUGUUCACUUCA--UCACCCu 5'
            |:|  |||| |  |||||| 
Target 5' taATATTTGAACTCAAGTGGGa 3'
 692 - 713 122.00 -12.00
2 
miRNA  3' aguGUUCACUUCAU-CACCCu 5'
             ||  |  | || ||||| 
Target 5' ttcCATCTTTAATATGTGGGa 3'
 72 - 92 113.00 -8.50
3 
miRNA  3' agUGUUCACUUC---AUCACCcu 5'
            |||  | |||   || |||  
Target 5' agACACTTTAAGTTATACTGGaa 3'
 15 - 37 102.00 -6.00
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30480481 6 COSMIC
COSN31600029 8 COSMIC
COSN30476219 43 COSMIC
COSN24388333 75 COSMIC
COSN31612803 75 COSMIC
COSN32064787 173 COSMIC
COSN8517716 193 COSMIC
COSN30171626 227 COSMIC
COSN31544371 235 COSMIC
COSN31606728 405 COSMIC
COSN25396901 414 COSMIC
COSN31569768 733 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs752608673 6 dbSNP
rs1354076243 8 dbSNP
rs755588788 10 dbSNP
rs1457512206 11 dbSNP
rs368406672 12 dbSNP
rs978882834 13 dbSNP
rs1394976869 15 dbSNP
rs748921321 18 dbSNP
rs770537633 19 dbSNP
rs1481062310 21 dbSNP
rs201138428 22 dbSNP
rs746218253 24 dbSNP
rs987725774 31 dbSNP
rs772633580 32 dbSNP
rs1254512380 38 dbSNP
rs775865352 41 dbSNP
rs761187572 43 dbSNP
rs1188494287 47 dbSNP
rs768641370 48 dbSNP
rs868270181 51 dbSNP
rs150064691 57 dbSNP
rs1180894778 58 dbSNP
rs1438021247 67 dbSNP
rs985831819 68 dbSNP
rs879176220 89 dbSNP
rs1211470924 92 dbSNP
rs1333453791 99 dbSNP
rs192493888 103 dbSNP
rs1234437432 106 dbSNP
rs1382042471 111 dbSNP
rs1278124730 115 dbSNP
rs1441987811 116 dbSNP
rs1336756817 119 dbSNP
rs760626264 121 dbSNP
rs1333559049 122 dbSNP
rs531651120 135 dbSNP
rs1297028342 136 dbSNP
rs1393740700 153 dbSNP
rs765392396 154 dbSNP
rs752673311 158 dbSNP
rs1407002997 160 dbSNP
rs908869115 161 dbSNP
rs931151190 171 dbSNP
rs1273787293 173 dbSNP
rs1470365527 174 dbSNP
rs551659229 178 dbSNP
rs1234415147 180 dbSNP
rs941800733 181 dbSNP
rs1467807786 187 dbSNP
rs1469621601 197 dbSNP
rs1206656530 202 dbSNP
rs971860038 216 dbSNP
rs918975833 217 dbSNP
rs1048192265 235 dbSNP
rs938539552 237 dbSNP
rs1056854129 238 dbSNP
rs1241730394 247 dbSNP
rs1475830977 248 dbSNP
rs1351780785 251 dbSNP
rs1188508938 253 dbSNP
rs763272822 258 dbSNP
rs1438974181 261 dbSNP
rs1411668972 269 dbSNP
rs1326631234 272 dbSNP
rs1438751814 278 dbSNP
rs764214530 279 dbSNP
rs1004559932 287 dbSNP
rs1343540368 294 dbSNP
rs1035608861 296 dbSNP
rs1401224230 297 dbSNP
rs1381936605 299 dbSNP
rs1171590521 304 dbSNP
rs895505476 305 dbSNP
rs1045075448 307 dbSNP
rs1420705410 315 dbSNP
rs751974948 317 dbSNP
rs145390195 323 dbSNP
rs890353538 333 dbSNP
rs527731998 337 dbSNP
rs185478022 343 dbSNP
rs1448141664 344 dbSNP
rs1031095709 346 dbSNP
rs190021603 356 dbSNP
rs778379692 368 dbSNP
rs1232614916 369 dbSNP
rs978937001 373 dbSNP
rs1304387787 375 dbSNP
rs1221176753 380 dbSNP
rs1290300835 381 dbSNP
rs1359881304 382 dbSNP
rs1031871731 384 dbSNP
rs1431373637 387 dbSNP
rs181650872 398 dbSNP
rs1362433193 408 dbSNP
rs536326137 413 dbSNP
rs757627292 421 dbSNP
rs1433828491 436 dbSNP
rs1293822789 441 dbSNP
rs13995 457 dbSNP
rs988169285 460 dbSNP
rs892508729 464 dbSNP
rs1336925235 478 dbSNP
rs1478420145 479 dbSNP
rs1266020073 482 dbSNP
rs912139917 484 dbSNP
rs943373183 490 dbSNP
rs1212509089 492 dbSNP
rs186460907 493 dbSNP
rs867182352 494 dbSNP
rs920699848 506 dbSNP
rs930702024 507 dbSNP
rs1291311023 508 dbSNP
rs1414111010 516 dbSNP
rs1283213166 529 dbSNP
rs1047791985 538 dbSNP
rs569881364 540 dbSNP
rs886980395 542 dbSNP
rs1030501317 545 dbSNP
rs112326175 547 dbSNP
rs1218151951 549 dbSNP
rs1170490885 552 dbSNP
rs1466695396 558 dbSNP
rs368112977 561 dbSNP
rs1186352350 566 dbSNP
rs895692741 567 dbSNP
rs1013016005 571 dbSNP
rs558941059 581 dbSNP
rs1479763384 587 dbSNP
rs1188832257 589 dbSNP
rs1250931047 590 dbSNP
rs1199536794 595 dbSNP
rs1320024137 598 dbSNP
rs750959803 600 dbSNP
rs1271357297 605 dbSNP
rs1420636490 612 dbSNP
rs1232601660 616 dbSNP
rs1335451818 618 dbSNP
rs986363836 619 dbSNP
rs571891324 622 dbSNP
rs1372381718 623 dbSNP
rs963089993 625 dbSNP
rs972106115 640 dbSNP
rs999968702 657 dbSNP
rs1391564081 666 dbSNP
rs1402854745 670 dbSNP
rs919019254 675 dbSNP
rs779661990 682 dbSNP
rs1031423949 690 dbSNP
rs1413611750 691 dbSNP
rs1417656089 697 dbSNP
rs1312891574 706 dbSNP
rs938430366 708 dbSNP
rs1356819629 709 dbSNP
rs956439299 713 dbSNP
rs776262077 716 dbSNP
rs1019620504 729 dbSNP
rs1213194364 735 dbSNP
rs1356920375 756 dbSNP
rs371578953 758 dbSNP
rs1236819843 765 dbSNP
rs974729802 765 dbSNP
rs948661543 766 dbSNP
rs566363168 767 dbSNP
rs1042970238 769 dbSNP
rs1276766477 770 dbSNP
rs1307444579 771 dbSNP
rs1347502401 774 dbSNP
rs1368397364 775 dbSNP
rs527977165 781 dbSNP
rs1440954472 784 dbSNP
rs952042614 785 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions Hela
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1048188. RNA binding protein: AGO2. Condition:Hela_AGO2_CLIP_ptb_knockdown ...

- Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al., 2013, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' aguguucacuucaUCACCCu 5'
                       |||||| 
Target 5' --------acucaAGUGGGa 3'
 		1 - 12
Article - Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al.
- Cell, 2013
The induction of pluripotency or trans-differentiation of one cell type to another can be accomplished with cell-lineage-specific transcription factors. Here, we report that repression of a single RNA binding polypyrimidine-tract-binding (PTB) protein, which occurs during normal brain development via the action of miR-124, is sufficient to induce trans-differentiation of fibroblasts into functional neurons. Besides its traditional role in regulated splicing, we show that PTB has a previously undocumented function in the regulation of microRNA functions, suppressing or enhancing microRNA targeting by competitive binding on target mRNA or altering local RNA secondary structure. A key event during neuronal induction is the relief of PTB-mediated blockage of microRNA action on multiple components of the REST complex, thereby derepressing a large array of neuronal genes, including miR-124 and multiple neuronal-specific transcription factors, in nonneuronal cells. This converts a negative feedback loop to a positive one to elicit cellular reprogramming to the neuronal lineage.
LinkOut: [PMID: 23313552]
CLIP-seq Support 1 for dataset GSM1048188
Method / RBP HITS-CLIP / AGO2
Cell line / Condition Hela / Hela_AGO2_CLIP_ptb_knockdown
Location of target site ENST00000248594.6 | 3UTR | ACUCAAGUGGGAUUAGAAGACUAUCAAAAUACAUGUA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23313552 / GSE42701
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
83 hsa-miR-4301 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT081134 LDLR low density lipoprotein receptor 2 4
MIRT090911 ARHGEF26 Rho guanine nucleotide exchange factor 26 2 2
MIRT229436 MECP2 methyl-CpG binding protein 2 2 2
MIRT274717 CPSF6 cleavage and polyadenylation specific factor 6 2 2
MIRT350957 BACH1 BTB domain and CNC homolog 1 2 2
MIRT386722 NUFIP2 NUFIP2, FMR1 interacting protein 2 2 2
MIRT446022 PEX3 peroxisomal biogenesis factor 3 2 2
MIRT481303 ATP5G3 ATP synthase, H+ transporting, mitochondrial Fo complex subunit C3 (subunit 9) 2 14
MIRT483959 ZNF354B zinc finger protein 354B 2 6
MIRT495667 TUBAL3 tubulin alpha like 3 2 2
MIRT496554 TBX15 T-box 15 2 2
MIRT497702 ARL6IP6 ADP ribosylation factor like GTPase 6 interacting protein 6 2 2
MIRT498105 RMND5A required for meiotic nuclear division 5 homolog A 2 2
MIRT512535 SEMA4D semaphorin 4D 2 2
MIRT512556 MFN2 mitofusin 2 2 6
MIRT521465 RABGAP1 RAB GTPase activating protein 1 2 6
MIRT526236 C2orf15 chromosome 2 open reading frame 15 2 2
MIRT526743 HLA-DOB major histocompatibility complex, class II, DO beta 2 2
MIRT527578 BRD7 bromodomain containing 7 2 4
MIRT528038 WT1 Wilms tumor 1 2 2
MIRT528267 GPRIN2 G protein regulated inducer of neurite outgrowth 2 2 2
MIRT528505 HTR7 5-hydroxytryptamine receptor 7 2 4
MIRT528758 RPS27 ribosomal protein S27 2 6
MIRT528999 IPO9 importin 9 2 2
MIRT529701 MRPL30 mitochondrial ribosomal protein L30 2 2
MIRT529781 C17orf82 chromosome 17 open reading frame 82 2 2
MIRT533797 TMEM119 transmembrane protein 119 2 6
MIRT535308 PHF12 PHD finger protein 12 2 2
MIRT535917 MKL2 MKL1/myocardin like 2 2 2
MIRT544892 OSBPL1A oxysterol binding protein like 1A 2 2
MIRT555423 PPIC peptidylprolyl isomerase C 2 2
MIRT562068 KLHL15 kelch like family member 15 2 2
MIRT565623 SLC31A1 solute carrier family 31 member 1 2 2
MIRT565661 SIX1 SIX homeobox 1 2 2
MIRT570138 IL1RL2 interleukin 1 receptor like 2 2 2
MIRT571045 YRDC yrdC N6-threonylcarbamoyltransferase domain containing 2 2
MIRT621232 LMAN1 lectin, mannose binding 1 2 2
MIRT622272 SH3TC2 SH3 domain and tetratricopeptide repeats 2 2 2
MIRT623844 GAN gigaxonin 2 2
MIRT626158 NFYA nuclear transcription factor Y subunit alpha 2 2
MIRT626464 CMKLR1 chemerin chemokine-like receptor 1 2 2
MIRT632087 ALDH1A2 aldehyde dehydrogenase 1 family member A2 2 2
MIRT637243 FAM26E calcium homeostasis modulator family member 5 2 2
MIRT642710 FGFR1OP2 FGFR1 oncogene partner 2 2 2
MIRT643611 KANSL3 KAT8 regulatory NSL complex subunit 3 2 2
MIRT644270 PAFAH1B1 platelet activating factor acetylhydrolase 1b regulatory subunit 1 2 2
MIRT645470 SPIN3 spindlin family member 3 2 2
MIRT649193 DNPEP aspartyl aminopeptidase 2 2
MIRT650776 POP4 POP4 homolog, ribonuclease P/MRP subunit 2 2
MIRT651345 ZC2HC1C zinc finger C2HC-type containing 1C 2 2
MIRT652237 TRAPPC3L trafficking protein particle complex 3 like 2 2
MIRT652587 TIMM8A translocase of inner mitochondrial membrane 8A 2 2
MIRT654591 PURA purine rich element binding protein A 2 2
MIRT654649 PTAFR platelet activating factor receptor 2 2
MIRT656842 KLF7 Kruppel like factor 7 2 2
MIRT657209 IKZF2 IKAROS family zinc finger 2 2 2
MIRT658513 ETV3 ETS variant 3 2 2
MIRT659448 CNNM2 cyclin and CBS domain divalent metal cation transport mediator 2 2 2
MIRT668816 CYLD CYLD lysine 63 deubiquitinase 2 2
MIRT669067 CELSR3 cadherin EGF LAG seven-pass G-type receptor 3 2 2
MIRT677711 ELOF1 elongation factor 1 homolog 2 4
MIRT687146 PTPN12 protein tyrosine phosphatase, non-receptor type 12 2 2
MIRT698498 THOC2 THO complex 2 2 2
MIRT707930 PPP1R3D protein phosphatase 1 regulatory subunit 3D 4 2
MIRT708737 FAM71F2 family with sequence similarity 71 member F2 2 2
MIRT710031 POLL DNA polymerase lambda 2 2
MIRT712175 STK4 serine/threonine kinase 4 2 2
MIRT715297 MAPK1 mitogen-activated protein kinase 1 2 2
MIRT716892 AGPAT6 glycerol-3-phosphate acyltransferase 4 2 2
MIRT717378 RBM41 RNA binding motif protein 41 2 2
MIRT718082 CLIC5 chloride intracellular channel 5 2 2
MIRT718721 ANKRD18A ankyrin repeat domain 18A 2 2
MIRT719152 DPYSL5 dihydropyrimidinase like 5 2 2
MIRT719224 CAMK4 calcium/calmodulin dependent protein kinase IV 2 2
MIRT719528 SRCIN1 SRC kinase signaling inhibitor 1 2 2
MIRT719861 KLF2 Kruppel like factor 2 2 2
MIRT720326 CAMK2G calcium/calmodulin dependent protein kinase II gamma 2 2
MIRT721596 SREBF1 sterol regulatory element binding transcription factor 1 2 2
MIRT722020 NEBL nebulette 2 2
MIRT722484 QSOX1 quiescin sulfhydryl oxidase 1 2 2
MIRT722614 TEAD1 TEA domain transcription factor 1 2 2
MIRT723664 RPTN repetin 2 2
MIRT723939 SVOP SV2 related protein 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-4 Dexamethasone approved 5743 Microarray primary rat thymocytes 20847043 2010 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-4301 Cisplatin 5460033 NSC119875 approved sensitive High Hepatocellular Carcinoma cell line (Hep3B, 97L)
hsa-miR-4301 Imatinib 5291 NSC743414 approved resistant High Chronic Myelogenous Leukemia cell line (K562)
hsa-mir-4301 Paclitaxel 36314 NSC125973 approved resistant cell line (BAS)
hsa-mir-4301 Paclitaxel 36314 NSC125973 approved sensitive cell line (W1)
hsa-mir-4301 Doxorubicin 31703 NSC123127 approved sensitive cell line (W1)
hsa-mir-4301 Methotrexate 126941 NSC740 approved sensitive cell line (W1)
hsa-mir-4301 Topotecan 60699 NSC609699 approved sensitive cell line (W1)
hsa-miR-4301 Gefitinib 123631 NSC715055 approved resistant cell line (PC9)
hsa-miR-4301 Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)

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