pre-miRNA Information
pre-miRNA hsa-mir-6132   
Genomic Coordinates chr7: 117020211 - 117020319
Description Homo sapiens miR-6132 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-6132
Sequence 21| AGCAGGGCUGGGGAUUGCA |39
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1272646274 4 dbSNP
rs1211969145 8 dbSNP
rs1310278496 11 dbSNP
rs1044115534 13 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol NIF3L1   
Synonyms ALS2CR1, CALS-7, MDS015
Description NGG1 interacting factor 3 like 1
Transcript NM_001142356   
Other Transcripts NM_001142355 , NM_001136039 , NM_021824   
Expression
Putative miRNA Targets on NIF3L1
3'UTR of NIF3L1
(miRNA target sites are highlighted)
>NIF3L1|NM_001142356|3'UTR
   1 ATGTCATCCTCTGTGAACACAGCAACACTGAACGAGGCTTTCTTTCTGACCTTCGAGATATGCTGGATTCTCACTTGGAG
  81 AATAAGATAAATATTATCCTATCAGAGACTGACAGGGACCCTCTTCAGGTGGTATAATTGCAGAAACATCAGGATAACAC
 161 ATTCTACAAATCAGCTGGATGCCAACTTAAATTTGTAACATGAGTCAGTGGGACTGGTGTGCTTCCAGAGAGTGTCTTCG
 241 AGGGTATCATCATTTCCGGTTTGTTAATCTTATTCACCAAATGTTCTATCGCTCGTAAGGTAAAACTGTAATATAACTAC
 321 CATATTAAATAACAAATGTTCATTATAAACTCTAGGAAAGATTGAATAAAATCTGTTTACTTAACATTCTTAAAAAAAAA
 401 AAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' acguuaggggucGGGACGa 5'
                      |:|||: 
Target 5' ----atgtcatcCTCTGTg 3'
1 - 15 88.00 -7.94
2
miRNA  3' acguuAG-GGGUCGGGACGa 5'
               || ::| | ||| | 
Target 5' ggcttTCTTTCTGACCTTCg 3'
36 - 55 82.00 -6.70
3
miRNA  3' acGUUAGGGGU---CGGGAcga 5'
            :| || |||   |::||   
Target 5' ctTATTCACCAAATGTTCTatc 3'
269 - 290 81.00 -5.40
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSM4090135 6 COSMIC
COSM8554297 30 COSMIC
COSM7563325 34 COSMIC
COSM8697411 35 COSMIC
COSM3990973 52 COSMIC
COSM2906791 56 COSMIC
COSM4457969 71 COSMIC
COSM9428481 78 COSMIC
COSM7793943 96 COSMIC
COSM5642794 104 COSMIC
COSM4090137 108 COSMIC
COSM8179667 112 COSMIC
COSM7144645 124 COSMIC
COSM7989624 124 COSMIC
COSM8625213 131 COSMIC
COSN31563538 183 COSMIC
COSN30551101 209 COSMIC
COSN31610299 210 COSMIC
COSN30503852 212 COSMIC
COSN30128304 233 COSMIC
COSN31581752 240 COSMIC
COSN30623980 241 COSMIC
COSN31533435 261 COSMIC
COSN8608354 307 COSMIC
COSN31572617 346 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs772171472 3 dbSNP
rs1264370535 4 dbSNP
rs567390588 7 dbSNP
rs765030134 9 dbSNP
rs1418160903 12 dbSNP
rs1160818535 15 dbSNP
rs1346162198 20 dbSNP
rs182064368 22 dbSNP
rs771569005 26 dbSNP
rs371020421 28 dbSNP
rs1454099324 29 dbSNP
rs1315215076 32 dbSNP
rs777077429 34 dbSNP
rs199508319 37 dbSNP
rs773192163 38 dbSNP
rs1412664725 39 dbSNP
rs774021292 42 dbSNP
rs1317317979 43 dbSNP
rs762866268 49 dbSNP
rs1254139024 51 dbSNP
rs1440554235 52 dbSNP
rs1462633432 53 dbSNP
rs994390127 55 dbSNP
rs761419088 56 dbSNP
rs767000597 61 dbSNP
rs749928883 62 dbSNP
rs1187525854 63 dbSNP
rs766294324 65 dbSNP
rs1441144897 73 dbSNP
rs753841759 75 dbSNP
rs754740309 79 dbSNP
rs1205138755 81 dbSNP
rs778730433 83 dbSNP
rs1361396137 85 dbSNP
rs748355781 86 dbSNP
rs1336745254 87 dbSNP
rs1352311401 93 dbSNP
rs758580511 102 dbSNP
rs777843189 106 dbSNP
rs1247672099 109 dbSNP
rs1280955961 109 dbSNP
rs1407645318 111 dbSNP
rs747071549 113 dbSNP
rs774532936 114 dbSNP
rs771193099 115 dbSNP
rs367931180 117 dbSNP
rs1325582988 120 dbSNP
rs1350717910 121 dbSNP
rs370636849 123 dbSNP
rs539765919 125 dbSNP
rs558074352 133 dbSNP
rs1298883998 139 dbSNP
rs775738194 141 dbSNP
rs1359975758 143 dbSNP
rs761482235 152 dbSNP
rs374296091 154 dbSNP
rs1426766002 160 dbSNP
rs1189838561 167 dbSNP
rs367570891 169 dbSNP
rs1481018982 172 dbSNP
rs1002664372 175 dbSNP
rs970460607 178 dbSNP
rs765917328 179 dbSNP
rs753755941 185 dbSNP
rs371643422 186 dbSNP
rs1003282542 188 dbSNP
rs1354736328 192 dbSNP
rs576115734 195 dbSNP
rs1435394276 196 dbSNP
rs1291505543 203 dbSNP
rs764978480 205 dbSNP
rs1487697383 207 dbSNP
rs752440212 209 dbSNP
rs758588079 211 dbSNP
rs1316043581 213 dbSNP
rs777844655 215 dbSNP
rs1273888532 216 dbSNP
rs747320358 218 dbSNP
rs757379692 221 dbSNP
rs781468537 222 dbSNP
rs746325720 223 dbSNP
rs752825552 225 dbSNP
rs1164538709 226 dbSNP
rs1261623064 227 dbSNP
rs764300686 227 dbSNP
rs1476705985 229 dbSNP
rs756282564 232 dbSNP
rs770451116 233 dbSNP
rs775854168 240 dbSNP
rs749606841 241 dbSNP
rs772711658 243 dbSNP
rs1367095190 244 dbSNP
rs1405306033 245 dbSNP
rs1297418031 246 dbSNP
rs1338333326 247 dbSNP
rs1432817994 251 dbSNP
rs184981231 252 dbSNP
rs915512165 253 dbSNP
rs555516983 258 dbSNP
rs1245826123 259 dbSNP
rs765372655 263 dbSNP
rs150758415 270 dbSNP
rs986138294 274 dbSNP
rs527419355 278 dbSNP
rs551837416 289 dbSNP
rs137926202 291 dbSNP
rs750982297 292 dbSNP
rs908602163 295 dbSNP
rs190012248 296 dbSNP
rs919324293 300 dbSNP
rs1434548631 307 dbSNP
rs1356659011 314 dbSNP
rs79365698 321 dbSNP
rs898645511 323 dbSNP
rs1048681272 325 dbSNP
rs1398665799 332 dbSNP
rs1179190162 336 dbSNP
rs888375141 341 dbSNP
rs1433336739 343 dbSNP
rs1012087482 346 dbSNP
rs748769188 357 dbSNP
rs1470931781 358 dbSNP
rs1238599387 369 dbSNP
rs1251831631 371 dbSNP
rs906249866 374 dbSNP
rs1162248606 376 dbSNP
rs1420640704 377 dbSNP
rs1210687638 386 dbSNP
rs1312895763 390 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions Hela
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1048187. RNA binding protein: AGO2. Condition:Hela_AGO2_CLIP_control ...

- Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al., 2013, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' acguuaggggucGGGACGa 5'
                      |||||| 
Target 5' -----------uCCCUGCa 3'
1 - 8
Article - Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al.
- Cell, 2013
The induction of pluripotency or trans-differentiation of one cell type to another can be accomplished with cell-lineage-specific transcription factors. Here, we report that repression of a single RNA binding polypyrimidine-tract-binding (PTB) protein, which occurs during normal brain development via the action of miR-124, is sufficient to induce trans-differentiation of fibroblasts into functional neurons. Besides its traditional role in regulated splicing, we show that PTB has a previously undocumented function in the regulation of microRNA functions, suppressing or enhancing microRNA targeting by competitive binding on target mRNA or altering local RNA secondary structure. A key event during neuronal induction is the relief of PTB-mediated blockage of microRNA action on multiple components of the REST complex, thereby derepressing a large array of neuronal genes, including miR-124 and multiple neuronal-specific transcription factors, in nonneuronal cells. This converts a negative feedback loop to a positive one to elicit cellular reprogramming to the neuronal lineage.
LinkOut: [PMID: 23313552]
CLIP-seq Support 1 for dataset GSM1048187
Method / RBP HITS-CLIP / AGO2
Cell line / Condition Hela / Hela_AGO2_CLIP_control
Location of target site ENST00000409588.1 | 3UTR | UCCCUGCAACCUCUGCCUCCCGG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23313552 / GSE42701
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
82 hsa-miR-6132 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT067294 NECAP1 NECAP endocytosis associated 1 2 10
MIRT100110 ABT1 activator of basal transcription 1 2 8
MIRT358583 CANX calnexin 2 2
MIRT445247 SEMA5A semaphorin 5A 2 2
MIRT445764 CCND3 cyclin D3 2 2
MIRT452388 LY6E lymphocyte antigen 6 family member E 2 4
MIRT452829 FAM131B family with sequence similarity 131 member B 2 2
MIRT453450 GLG1 golgi glycoprotein 1 2 2
MIRT455435 ID3 inhibitor of DNA binding 3, HLH protein 2 2
MIRT460629 IGFBP4 insulin like growth factor binding protein 4 2 2
MIRT461607 DPH2 DPH2 homolog 2 2
MIRT461989 PACSIN1 protein kinase C and casein kinase substrate in neurons 1 2 2
MIRT464258 VCL vinculin 2 2
MIRT465713 TNFAIP1 TNF alpha induced protein 1 2 2
MIRT466475 TECPR2 tectonin beta-propeller repeat containing 2 2 7
MIRT467119 SRGAP1 SLIT-ROBO Rho GTPase activating protein 1 2 4
MIRT468299 SFT2D2 SFT2 domain containing 2 2 2
MIRT469696 RAB5B RAB5B, member RAS oncogene family 2 8
MIRT469904 PTRF caveolae associated protein 1 2 2
MIRT470015 PTPLB 3-hydroxyacyl-CoA dehydratase 2 1 1
MIRT471385 PDPR pyruvate dehydrogenase phosphatase regulatory subunit 2 2
MIRT471409 PDP2 pyruvate dehyrogenase phosphatase catalytic subunit 2 2 2
MIRT471719 OTUB1 OTU deubiquitinase, ubiquitin aldehyde binding 1 2 2
MIRT473608 MARK2 microtubule affinity regulating kinase 2 2 2
MIRT476328 GLTSCR1L BRD4 interacting chromatin remodeling complex associated protein like 2 2
MIRT479448 CDK6 cyclin dependent kinase 6 2 2
MIRT482032 AMER1 APC membrane recruitment protein 1 2 2
MIRT482360 AGO2 argonaute 2, RISC catalytic component 2 2
MIRT484661 HOXD3 homeobox D3 2 4
MIRT486828 NDOR1 NADPH dependent diflavin oxidoreductase 1 2 2
MIRT487069 CLASP1 cytoplasmic linker associated protein 1 2 4
MIRT487196 NFASC neurofascin 2 4
MIRT487448 TFAP2B transcription factor AP-2 beta 2 4
MIRT487517 GXYLT2 glucoside xylosyltransferase 2 2 2
MIRT487640 BRSK2 BR serine/threonine kinase 2 2 4
MIRT487755 SKI SKI proto-oncogene 2 4
MIRT489944 CPLX1 complexin 1 2 2
MIRT491101 MSI1 musashi RNA binding protein 1 2 4
MIRT491181 LAMA5 laminin subunit alpha 5 2 2
MIRT492355 SEMA7A semaphorin 7A (John Milton Hagen blood group) 2 2
MIRT493918 FAM127B retrotransposon Gag like 8A 2 4
MIRT493932 FAM127A retrotransposon Gag like 8C 2 4
MIRT494679 ARID3A AT-rich interaction domain 3A 2 2
MIRT494814 AKAP11 A-kinase anchoring protein 11 2 2
MIRT494835 ADCY9 adenylate cyclase 9 2 2
MIRT495455 PNMAL2 paraneoplastic Ma antigen family member 8B 2 2
MIRT496962 MAP1LC3B microtubule associated protein 1 light chain 3 beta 2 2
MIRT526228 MTRNR2L5 MT-RNR2-like 5 2 2
MIRT531028 TDGF1P3 teratocarcinoma-derived growth factor 1 pseudogene 3 2 2
MIRT557110 HOXA3 homeobox A3 2 2
MIRT560514 POGK pogo transposable element derived with KRAB domain 2 2
MIRT567753 DLC1 DLC1 Rho GTPase activating protein 2 2
MIRT569608 TRIM29 tripartite motif containing 29 2 2
MIRT570242 CPNE5 copine 5 2 2
MIRT572327 HSPB6 heat shock protein family B (small) member 6 2 2
MIRT572373 ATOX1 antioxidant 1 copper chaperone 2 2
MIRT575024 Tecpr2 tectonin beta-propeller repeat containing 2 2 5
MIRT576146 Hmox1 heme oxygenase 1 2 2
MIRT612443 SMOC2 SPARC related modular calcium binding 2 2 2
MIRT615404 VDAC2 voltage dependent anion channel 2 2 2
MIRT629114 CYCS cytochrome c, somatic 2 2
MIRT631362 FOXI2 forkhead box I2 2 2
MIRT639322 THBD thrombomodulin 2 2
MIRT643797 ABCC12 ATP binding cassette subfamily C member 12 2 2
MIRT669687 ABLIM1 actin binding LIM protein 1 2 2
MIRT670595 LLGL1 LLGL1, scribble cell polarity complex component 2 4
MIRT691190 NIF3L1 NGG1 interacting factor 3 like 1 2 2
MIRT691688 FLOT2 flotillin 2 2 2
MIRT697127 OTUD5 OTU deubiquitinase 5 2 2
MIRT700814 PHLDA2 pleckstrin homology like domain family A member 2 2 2
MIRT701248 NUP35 nucleoporin 35 2 2
MIRT702362 KLHL15 kelch like family member 15 2 2
MIRT703325 GDPD5 glycerophosphodiester phosphodiesterase domain containing 5 2 2
MIRT706060 PKD1 polycystin 1, transient receptor potential channel interacting 2 2
MIRT710475 CDH5 cadherin 5 2 2
MIRT713018 SLC4A2 solute carrier family 4 member 2 2 2
MIRT716427 RAB15 RAB15, member RAS oncogene family 2 2
MIRT718093 ABHD12 abhydrolase domain containing 12 2 2
MIRT718542 PIGQ phosphatidylinositol glycan anchor biosynthesis class Q 2 2
MIRT719122 CACFD1 calcium channel flower domain containing 1 2 2
MIRT721393 LDLRAD4 low density lipoprotein receptor class A domain containing 4 2 2
MIRT736283 CDC42 cell division cycle 42 2 0
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-6132 Vemurafenib 42611257 NSC761431 approved resistant High Melanoma cell line (A375, IGR37, 501Mel)
hsa-miR-6132 Dabrafenib 44462760 NSC764134 approved resistant High Melanoma cell line (A375, IGR37, 501Mel)
hsa-miR-6132 Paclitaxel 36314 NSC125973 approved resistant High Non-Small Cell Lung Cancer cell line (A549)
hsa-miR-6132 Cisplatin 5460033 NSC119875 approved resistant High Hypopharyngeal Cancer cell line (FaDu)
hsa-miR-6132 Temozolomide 5394 NSC362856 approved sensitive cell line (U251)
hsa-miR-6132 Cisplatin 5460033 NSC119875 approved sensitive cell line
hsa-miR-6132 Doxorubicin 31703 NSC123127 approved resistant cell line (HS578T)
hsa-miR-6132 Osimertinib 71496458 NSC779217 approved resistant cell line (H1975)
hsa-miR-6132 Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (100 ng/ml)

Error report submission