miRTarBase - #MIRT692492


—
pre-miRNA Information
pre-miRNA hsa-mir-758   
Genomic Coordinates chr14: 101026020 - 101026107
Synonyms MIRN758, hsa-mir-758, MIR758
Description Homo sapiens miR-758 stem-loop
Comment None
RNA Secondary Structure
Associated Diseases

Mature miRNA Information
Mature miRNA hsa-miR-758-5p
Sequence 15| GAUGGUUGACCAGAGAGCACAC |36
Evidence Experimental
Experiments SOLiD
Editing Events in miRNAs
Modification Type Position on miR Chromosome DNA Strand Genomic Position (hg38) List of PMIDs Variant details
A-to-I 16 14 + 101026049 29233923 MiREDiBase
A-to-I 21 14 + 101026054 29233923 MiREDiBase
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs372417522 1 dbSNP
rs748092616 2 dbSNP
rs1270901332 4 dbSNP
rs1364206491 5 dbSNP
rs1483583954 8 dbSNP
rs770931807 10 dbSNP
rs776760808 11 dbSNP
rs1360350664 12 dbSNP
rs759742151 13 dbSNP
rs1002938972 15 dbSNP
rs770107042 17 dbSNP
rs369242818 20 dbSNP
rs1217090272 21 dbSNP
rs763341052 22 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol SPIN4   
Synonyms TDRD28
Description spindlin family member 4
Transcript NM_001012968   
Expression
Putative miRNA Targets on SPIN4
3'UTR of SPIN4
(miRNA target sites are highlighted)		 
>SPIN4|NM_001012968|3'UTR
   1 ATTCTTTGTGCTCTTTAGAGAAGTTGTGGATCTGTTAGATGTGAATTATTTTGTGTTCTCGTAGTTGTGAACGCAGAGAA
  81 GAGTTTAGGTGTCAGAAATTCAGGAAAGTGGATAAATTTTATTGTGCCAACAAATCTTACCTTGACTAGTTTCACAATTT
 161 TGCCTCAAGTGTACTTTGCTACTTTTGATATTGCCTTGTTCTGTAACTTAACAGTTAAATTGGGTGCTAATAGAAAATTA
 241 AAAAGTGTTTGCAACCATTGGAACAATGCAAAAATAGATTAAGAAAAATTAATGGTGTAAACCCACACCCCGTCCTTACC
 321 CCTTCCTTTCCTTACCTTGTTTCTTCCCAGAAAACAAGTGTTGAGGATGCGAAGTACATCCTTCCACCTATTTTCCCCAT
 401 CTTCATGCAGAAAGTATGTTCTAGTAGTTTCTGTAAAAATGGCAAGCCACATGCTTTTCTCACTTGACATGTAGATGATA
 481 TCTCTTCTTATCAGTAGCTTCAGTCTTTCTTGCTCTCTCTGGCTCTCTCTGGCCCTCACTCTTACTCTCTTGCACTCTTG
 561 CTCTCCCCCTCCCTCCCTTACTCTAACAGCTGAATACACAGTACTTGTTGATGTACCAAAAAGGCATTCAACCATTTCGT
 641 TATTGATCGTCTTTTATTTCCTTTTTTTATTTTTTTTCAAAATAACACTGCTGTAATACATACTGGTGCTTTTATTTATA
 721 TAGGTTGGATTCCCAGAAATACTATGTAGGCATATGTTTCAGAAATATTACAAGATTACTATCCAAAATGAAAGCGCCCG
 801 TTCCAGCGTCCTCTCCAGCACTCTTTAATGCTTGAAAATATGAAAGGCTAAAAATTGCATTGTATTGCTTGTTAGTTTAA
 881 TTGCCTTTTGTCTGAGTTCAAAGAGGTTTGTTGTTGTTGCTGTCCTTGTTTCTGTTTGGATTTTTCTCTGATTTGCCTGT
 961 TCTTACCCTTTGCTCATGTATCTATTGATGTTTTTCATTATAAATTTGTAGAAATTCTTACTACATAGTGCATATTAATC
1041 ATTTGTAGCTCTAATGTGTTATGACGTTTTCATCTTGACTTTATTGATGATATATTTTCCTTTAAAATTTTTAAACTTTT
1121 TAGGTAGACCCTTATCTCTTTTATAGATTTTGGTTTCCTTTTTTTATCACATGGATGCCCCCACCCAAAGTCATACAAAT
1201 ATTCACCTACATTTTCTTCTAATAACTTTATTATTTTTAATGTTTAAATGTTTGATCCATCTGAAATTTATTTTTGTATA
1281 TGGTGTAAGATGGGGATCCAGCTATAACATTTTGTTCACATTGGATACCTGATTGTGACATTTATTTAAAATGTTACCCA
1361 TTTTCAAATTTCTGAGCCAATATCATGATTTAATTATAGTGGCTTCATCGTAAGTTTTAGAATCCGATAAAGCAAGTCCC
1441 ACTTCATTAGTTTTTTTTTTCTTTATATAATATGTCCTAGACATTCATTTTTTCATGTGAAAAAATGAAATGCAGAATTT
1521 TAATAAAATTCTAATTATGATGGCTGACATCACAATTAAAATCCTGCATTTTTGTTTAGAGGGCTCTTTAATAATATTAA
1601 ATCTTAGCACTCAAGAGTCTTCGTACATCATTGAAATCTTTTGGTCTTGTTATTGGAATATTCTTCACGTAAGTATATCA
1681 TAGCTAACTGAATTTATTTCTAAGTATTTTTACAGTTTTATTTCATATTTTGACATTGTGAATTGGTTTTTTTCTTCTCA
1761 TTTGTAATTAGCTATTTTTGACAATATAGACAACCTATAGATTTTTGTATTTTTATCTTCTGTCTAATGAGCTTATTTAA
1841 TCATTGAAATTACAGTAGATTTTTAAGAATATAATATTTTGGGTTTTCTAGATATGAAATTATTTCACCTGAACATATAG
1921 ATCATTTTATTTCCTCTTAATGTTTATACTGATTACTGTTACATTAGCTGGATTTTTCAAAACAATGTTGAAGAGTGATG
2001 ACAGACGTGACTGTCTTGTTCTTAATTTTCATGGAAGTAAGAATGCAAAATATTAATAGGGAATAGTATTCCCTATTAGT
2081 ATGACATTTACTTTTGGTTATTAGTAGGTAGTCATTAACATGTTTAAGAGTTTCCGCTATTCCTGTTTTATAGTGTTATT
2161 GCTAGAAGTGGTTCCTGAATTTTATAAAATGCCTTTTCAGCATCTATTGATAAAATTGTATGATTTTTTTTCTCTTTAAT
2241 TTGTTGATGTAATGAATTAGAATGGTAGGCATTTGATGTGGAACCAAACTTGTATTTCTGGAACAAATACTACTTGGTCA
2321 TTGTGAAATAATGATTTGCTACATGAGTGGATTTTATTTACCAGTATTTAATTTAGAATTATTGCATTTTCATTCCAAAG
2401 TAAAATTGTATTTTGTCCTTCTGATGCTATCTTTATCAAGTTTTGTTATTGAAGTTGTGCTAGCTTTAAAAAAATATGAA
2481 TATGTGAGTTTTCAATTTTTTTCCTGTGGTCTGGAATAATTTAAATAGCATTAGAATTATTTGTTTGTTAAAAGTTAGCT
2561 AGGACAAAGTGTAAAACAATCTAATCCTGATGACACTTTTAATAGTAGATTTTTAATCAGCTTTGTGATTTCTTTAATAG
2641 TTATTGGTTTATTCAGGCTTTTTATTAAAAAGGTATATACATTCTCTGTGACATACAAAGTTAAAAGTTCTCTATTATTG
2721 CTGATTGTACTGGTTGTTGCTTGTTGACTGTACTATTCAGATGGCTTTATGCTTTTGTGTATTTTATGTCACCTAGATCT
2801 AATTCTGAAAACATTGTAATAAAATAATTAGCTATAATGGTA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' caCACGAGAGACCAGUUGGUAg 5'
            |||   |:||  ||||||| 
Target 5' aaGTG---TTTG--CAACCATt 3'
 243 - 259 149.00 -13.00
2 
miRNA  3' cacacgagagaccAGUUGGUAg 5'
                       |||||||| 
Target 5' accaaaaaggcatTCAACCATt 3'
 615 - 636 145.00 -10.30
3 
miRNA  3' caCACGAGAGACCAGUUGGUAg 5'
            | ||| | |  |:||:||| 
Target 5' atGAGCT-TAT--TTAATCATt 3'
 1827 - 1845 119.00 -7.20
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1244160507 4 dbSNP
rs782722677 8 dbSNP
rs781942809 18 dbSNP
rs782317403 20 dbSNP
rs192545575 24 dbSNP
rs1354993404 32 dbSNP
rs1215748380 38 dbSNP
rs782450496 49 dbSNP
rs1483932939 60 dbSNP
rs879983847 63 dbSNP
rs781890822 73 dbSNP
rs1242138153 87 dbSNP
rs1442398118 103 dbSNP
rs1180214769 108 dbSNP
rs1380706600 109 dbSNP
rs1418328016 115 dbSNP
rs188779070 128 dbSNP
rs1360883131 130 dbSNP
rs1467645424 132 dbSNP
rs1337480515 134 dbSNP
rs782679493 143 dbSNP
rs1311868548 144 dbSNP
rs1450755364 144 dbSNP
rs782650921 146 dbSNP
rs1378066753 149 dbSNP
rs1223626424 171 dbSNP
rs782501799 178 dbSNP
rs782373622 194 dbSNP
rs1314531036 195 dbSNP
rs1233254786 223 dbSNP
rs1261980550 224 dbSNP
rs184860541 229 dbSNP
rs1459661797 234 dbSNP
rs192451664 240 dbSNP
rs1200173691 246 dbSNP
rs1238460771 247 dbSNP
rs1480180858 251 dbSNP
rs1175332281 260 dbSNP
rs1427098147 261 dbSNP
rs1433718680 264 dbSNP
rs368515222 267 dbSNP
rs1428724630 276 dbSNP
rs1333589094 290 dbSNP
rs1355391956 292 dbSNP
rs1441923181 296 dbSNP
rs1370286844 302 dbSNP
rs1220337741 308 dbSNP
rs1282043054 309 dbSNP
rs1320758524 316 dbSNP
rs1220003383 344 dbSNP
rs782453876 350 dbSNP
rs1258301297 357 dbSNP
rs1306567020 358 dbSNP
rs1202113503 365 dbSNP
rs782227856 371 dbSNP
rs1248269227 375 dbSNP
rs1488745255 376 dbSNP
rs782764914 379 dbSNP
rs1266742047 381 dbSNP
rs782046345 386 dbSNP
rs1479488259 388 dbSNP
rs781805044 398 dbSNP
rs1420408955 414 dbSNP
rs1409620869 416 dbSNP
rs1168714840 427 dbSNP
rs782801354 440 dbSNP
rs782615391 442 dbSNP
rs782517611 451 dbSNP
rs187487099 471 dbSNP
rs144874474 482 dbSNP
rs1270286749 484 dbSNP
rs1360784241 490 dbSNP
rs374151641 493 dbSNP
rs1322082145 495 dbSNP
rs1221539551 496 dbSNP
rs782175026 498 dbSNP
rs1289493880 521 dbSNP
rs1489231511 523 dbSNP
rs1195860116 525 dbSNP
rs1242797511 527 dbSNP
rs1184969244 529 dbSNP
rs1416290422 534 dbSNP
rs182810226 534 dbSNP
rs1163347615 538 dbSNP
rs1364190924 541 dbSNP
rs1422088332 545 dbSNP
rs781801620 551 dbSNP
rs1325323738 553 dbSNP
rs1428105362 554 dbSNP
rs371693550 555 dbSNP
rs1285573572 565 dbSNP
rs1383153962 568 dbSNP
rs1244885010 577 dbSNP
rs1312658955 585 dbSNP
rs1355703684 598 dbSNP
rs1205676695 615 dbSNP
rs1283524908 617 dbSNP
rs1446477807 623 dbSNP
rs1213816653 624 dbSNP
rs782686008 638 dbSNP
rs1239364214 639 dbSNP
rs1445484857 648 dbSNP
rs1183319919 652 dbSNP
rs1383815213 661 dbSNP
rs192749241 668 dbSNP
rs1159634932 669 dbSNP
rs781948547 670 dbSNP
rs1384951512 673 dbSNP
rs782789539 678 dbSNP
rs1335622363 695 dbSNP
rs1406278078 702 dbSNP
rs1451486696 704 dbSNP
rs1378794103 714 dbSNP
rs1239563791 719 dbSNP
rs1278870045 732 dbSNP
rs1348462149 739 dbSNP
rs1222088138 740 dbSNP
rs1265105088 745 dbSNP
rs1461915191 749 dbSNP
rs1203239503 751 dbSNP
rs1241589825 754 dbSNP
rs1459361959 771 dbSNP
rs1183068176 775 dbSNP
rs1363656504 794 dbSNP
rs1469062956 795 dbSNP
rs1173766485 796 dbSNP
rs1468869058 799 dbSNP
rs1334259849 800 dbSNP
rs782109715 813 dbSNP
rs1402092272 826 dbSNP
rs1391693432 827 dbSNP
rs868908142 834 dbSNP
rs1333860882 851 dbSNP
rs1438565208 857 dbSNP
rs188129702 874 dbSNP
rs1283898694 879 dbSNP
rs1324351085 881 dbSNP
rs1223048874 905 dbSNP
rs1261496790 911 dbSNP
rs782140103 920 dbSNP
rs1196685550 934 dbSNP
rs1250282483 944 dbSNP
rs1436535761 952 dbSNP
rs1202185104 956 dbSNP
rs1270294029 966 dbSNP
rs1479816800 971 dbSNP
rs1173719834 973 dbSNP
rs1425257232 1003 dbSNP
rs1462199129 1011 dbSNP
rs1167067257 1013 dbSNP
rs1394033334 1020 dbSNP
rs1406928110 1032 dbSNP
rs1303679784 1045 dbSNP
rs782327916 1049 dbSNP
rs1404336217 1051 dbSNP
rs1281466883 1062 dbSNP
rs1328369979 1066 dbSNP
rs1228465440 1073 dbSNP
rs1251007928 1074 dbSNP
rs1337828956 1083 dbSNP
rs1225258921 1084 dbSNP
rs182615275 1089 dbSNP
rs1293133171 1092 dbSNP
rs782398389 1093 dbSNP
rs1196590164 1112 dbSNP
rs1263262386 1119 dbSNP
rs781969931 1127 dbSNP
rs1192001229 1129 dbSNP
rs1426316750 1130 dbSNP
rs1163990434 1132 dbSNP
rs1422689387 1135 dbSNP
rs1301325256 1153 dbSNP
rs190868338 1160 dbSNP
rs1295107950 1166 dbSNP
rs1382894093 1166 dbSNP
rs781962476 1174 dbSNP
rs1248537184 1178 dbSNP
rs782302994 1180 dbSNP
rs782186707 1181 dbSNP
rs1263068388 1184 dbSNP
rs185270303 1190 dbSNP
rs1485902991 1214 dbSNP
rs1204112230 1219 dbSNP
rs1259154922 1223 dbSNP
rs1446091326 1232 dbSNP
rs1183915109 1261 dbSNP
rs1384409874 1280 dbSNP
rs1442585690 1294 dbSNP
rs1364368845 1298 dbSNP
rs1454370231 1310 dbSNP
rs1288367152 1353 dbSNP
rs1383003602 1358 dbSNP
rs1454811637 1397 dbSNP
rs1337686879 1399 dbSNP
rs183552163 1402 dbSNP
rs1244313909 1409 dbSNP
rs1309784060 1410 dbSNP
rs12859290 1411 dbSNP
rs1237639606 1412 dbSNP
rs1318056829 1412 dbSNP
rs1258452851 1415 dbSNP
rs6653244 1425 dbSNP
rs1203872366 1433 dbSNP
rs1242195588 1434 dbSNP
rs1459801836 1439 dbSNP
rs1178799037 1440 dbSNP
rs1408538481 1448 dbSNP
rs1409811259 1461 dbSNP
rs1418445712 1461 dbSNP
rs1471025867 1461 dbSNP
rs1177404078 1466 dbSNP
rs1405716703 1474 dbSNP
rs1446191950 1477 dbSNP
rs1300284899 1481 dbSNP
rs111923761 1482 dbSNP
rs1374619105 1483 dbSNP
rs1391350509 1487 dbSNP
rs1305541337 1495 dbSNP
rs1325041485 1506 dbSNP
rs1223655718 1515 dbSNP
rs1262114471 1523 dbSNP
rs1320977306 1531 dbSNP
rs1206283667 1540 dbSNP
rs1233275089 1548 dbSNP
rs1480211050 1550 dbSNP
rs1175391983 1557 dbSNP
rs1271039467 1563 dbSNP
rs1481458567 1574 dbSNP
rs1200240724 1577 dbSNP
rs1428791979 1583 dbSNP
rs1413007460 1584 dbSNP
rs782672498 1587 dbSNP
rs1355444166 1592 dbSNP
rs1461837523 1594 dbSNP
rs1306700641 1607 dbSNP
rs1348004950 1611 dbSNP
rs35386099 1617 dbSNP
rs1282105262 1622 dbSNP
rs1343202071 1623 dbSNP
rs1215929645 1624 dbSNP
rs1273332820 1626 dbSNP
rs190939032 1629 dbSNP
rs1202268384 1668 dbSNP
rs782577854 1669 dbSNP
rs1490498107 1673 dbSNP
rs1223257147 1699 dbSNP
rs1268302481 1712 dbSNP
rs1477106876 1733 dbSNP
rs1188486662 1736 dbSNP
rs1420568094 1746 dbSNP
rs140248668 1747 dbSNP
rs1167106600 1754 dbSNP
rs1367942650 1755 dbSNP
rs1425871925 1760 dbSNP
rs1303123600 1764 dbSNP
rs782650579 1767 dbSNP
rs782489007 1790 dbSNP
rs1270403950 1807 dbSNP
rs35601060 1807 dbSNP
rs1360840435 1808 dbSNP
rs1225826487 1817 dbSNP
rs1316916997 1819 dbSNP
rs1360010463 1829 dbSNP
rs1221617941 1830 dbSNP
rs1289570881 1832 dbSNP
rs1218125635 1836 dbSNP
rs1242852041 1851 dbSNP
rs1485673743 1865 dbSNP
rs1387507939 1867 dbSNP
rs781791037 1872 dbSNP
rs1163490230 1879 dbSNP
rs1412348444 1884 dbSNP
rs1401871878 1887 dbSNP
rs1318419599 1888 dbSNP
rs1344185988 1893 dbSNP
rs201417572 1913 dbSNP
rs186214876 1914 dbSNP
rs1432483803 1917 dbSNP
rs1338347940 1923 dbSNP
rs782742508 1932 dbSNP
rs1383234346 1968 dbSNP
rs1245052798 1971 dbSNP
rs782509645 1973 dbSNP
rs1282354068 1979 dbSNP
rs1352757869 1994 dbSNP
rs781854712 2001 dbSNP
rs782802724 2006 dbSNP
rs782070999 2007 dbSNP
rs181706942 2008 dbSNP
rs1328703282 2021 dbSNP
rs1207033082 2025 dbSNP
rs1245261038 2037 dbSNP
rs1462041844 2040 dbSNP
rs367922784 2045 dbSNP
rs1183370395 2051 dbSNP
rs73522538 2057 dbSNP
rs1471452896 2065 dbSNP
rs1158595894 2066 dbSNP
rs1406563679 2071 dbSNP
rs143309680 2072 dbSNP
rs781906335 2084 dbSNP
rs1178132104 2086 dbSNP
rs1406452803 2114 dbSNP
rs1451563898 2119 dbSNP
rs1331967838 2126 dbSNP
rs1337412355 2134 dbSNP
rs782766561 2135 dbSNP
rs1278928851 2136 dbSNP
rs1328558759 2153 dbSNP
rs1226720920 2155 dbSNP
rs782007812 2175 dbSNP
rs1265157275 2192 dbSNP
rs1324550116 2202 dbSNP
rs1200186674 2204 dbSNP
rs139546496 2214 dbSNP
rs1439956863 2216 dbSNP
rs1183132330 2218 dbSNP
rs76707308 2219 dbSNP
rs1471323185 2220 dbSNP
rs1200966382 2224 dbSNP
rs1429485238 2232 dbSNP
rs782298511 2232 dbSNP
rs1170554788 2234 dbSNP
rs1398327029 2252 dbSNP
rs1410380464 2264 dbSNP
rs1328095545 2270 dbSNP
rs1348560526 2283 dbSNP
rs782023441 2289 dbSNP
rs1285295655 2303 dbSNP
rs1346341430 2304 dbSNP
rs1232763071 2309 dbSNP
rs1273341804 2311 dbSNP
rs1341901322 2320 dbSNP
rs1196815509 2329 dbSNP
rs1274469009 2332 dbSNP
rs1482369682 2341 dbSNP
rs781829058 2343 dbSNP
rs1224012082 2361 dbSNP
rs1269016420 2361 dbSNP
rs1432135014 2376 dbSNP
rs1374504521 2391 dbSNP
rs189594305 2397 dbSNP
rs1167120511 2416 dbSNP
rs1368575944 2427 dbSNP
rs1426505875 2448 dbSNP
rs1303728083 2454 dbSNP
rs1366244035 2467 dbSNP
rs1386338476 2473 dbSNP
rs1299464080 2474 dbSNP
rs782315796 2475 dbSNP
rs1228543707 2476 dbSNP
rs1297355405 2477 dbSNP
rs1053713 2480 dbSNP
rs782176758 2481 dbSNP
rs1340610858 2486 dbSNP
rs1248765672 2489 dbSNP
rs536589640 2495 dbSNP
rs782612707 2501 dbSNP
rs1487870475 2504 dbSNP
rs1207555170 2509 dbSNP
rs1263328479 2511 dbSNP
rs782750711 2515 dbSNP
rs1187650130 2518 dbSNP
rs1388053483 2521 dbSNP
rs1446269945 2540 dbSNP
rs1164114727 2545 dbSNP
rs1384608327 2548 dbSNP
rs782738238 2558 dbSNP
rs150990347 2560 dbSNP
rs868922729 2562 dbSNP
rs782038255 2563 dbSNP
rs1383022841 2565 dbSNP
rs1320193145 2566 dbSNP
rs1363442170 2568 dbSNP
rs1454970122 2579 dbSNP
rs186348587 2581 dbSNP
rs181249499 2589 dbSNP
rs1316263222 2592 dbSNP
rs1321658075 2594 dbSNP
rs1241923677 2596 dbSNP
rs1350811244 2603 dbSNP
rs1207684519 2615 dbSNP
rs111287985 2619 dbSNP
rs782564137 2625 dbSNP
rs1183978515 2632 dbSNP
rs1255318988 2638 dbSNP
rs1422013232 2639 dbSNP
rs1162724603 2643 dbSNP
rs1365578534 2648 dbSNP
rs781936109 2657 dbSNP
rs1457881797 2667 dbSNP
rs1158691165 2675 dbSNP
rs1386733554 2677 dbSNP
rs1454862309 2679 dbSNP
rs1337826634 2686 dbSNP
rs1379030760 2697 dbSNP
rs1394765714 2709 dbSNP
rs1309843407 2714 dbSNP
rs1335814638 2720 dbSNP
rs1227312377 2729 dbSNP
rs781883839 2737 dbSNP
rs1265782364 2744 dbSNP
rs1325265001 2765 dbSNP
rs782580372 2786 dbSNP
rs1204009744 2799 dbSNP
rs370236859 2814 dbSNP
rs1255229911 2829 dbSNP
rs1482177274 2831 dbSNP
rs782487303 2836 dbSNP
rs1178858729 2840 dbSNP
rs1251231306 2842 dbSNP
rs1472957402 2843 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions Hela
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1048187. RNA binding protein: AGO2. Condition:Hela_AGO2_CLIP_control ...

- Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al., 2013, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' cacacgagagaccAGUUGGUAg 5'
                       |||||||| 
Target 5' -------aggcauUCAACCAUu 3'
 		1 - 15
Article - Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al.
- Cell, 2013
The induction of pluripotency or trans-differentiation of one cell type to another can be accomplished with cell-lineage-specific transcription factors. Here, we report that repression of a single RNA binding polypyrimidine-tract-binding (PTB) protein, which occurs during normal brain development via the action of miR-124, is sufficient to induce trans-differentiation of fibroblasts into functional neurons. Besides its traditional role in regulated splicing, we show that PTB has a previously undocumented function in the regulation of microRNA functions, suppressing or enhancing microRNA targeting by competitive binding on target mRNA or altering local RNA secondary structure. A key event during neuronal induction is the relief of PTB-mediated blockage of microRNA action on multiple components of the REST complex, thereby derepressing a large array of neuronal genes, including miR-124 and multiple neuronal-specific transcription factors, in nonneuronal cells. This converts a negative feedback loop to a positive one to elicit cellular reprogramming to the neuronal lineage.
LinkOut: [PMID: 23313552]
CLIP-seq Support 1 for dataset GSM1048187
Method / RBP HITS-CLIP / AGO2
Cell line / Condition Hela / Hela_AGO2_CLIP_control
Location of target site ENST00000374884.2 | 3UTR | AGGCAUUCAACCAUUUCGUUAUUGAUCGUCUUUUAU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23313552 / GSE42701
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
62 hsa-miR-758-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT085323 MORC3 MORC family CW-type zinc finger 3 2 2
MIRT089441 STAMBP STAM binding protein 2 2
MIRT089456 TET3 tet methylcytosine dioxygenase 3 2 2
MIRT111856 CCND1 cyclin D1 2 2
MIRT184933 ZNF268 zinc finger protein 268 2 2
MIRT215288 CREBRF CREB3 regulatory factor 2 2
MIRT237300 LPP LIM domain containing preferred translocation partner in lipoma 2 2
MIRT238446 MYO10 myosin X 2 4
MIRT273827 RPL41 ribosomal protein L41 2 2
MIRT282703 HOOK1 hook microtubule tethering protein 1 2 2
MIRT347970 ZNF850 zinc finger protein 850 2 2
MIRT371076 KLF3 Kruppel like factor 3 2 2
MIRT464339 USP6NL USP6 N-terminal like 2 2
MIRT470034 PTP4A1 protein tyrosine phosphatase type IVA, member 1 2 2
MIRT477506 ELL2 elongation factor for RNA polymerase II 2 2 2
MIRT482886 CACNA2D3 calcium voltage-gated channel auxiliary subunit alpha2delta 3 2 2
MIRT492606 POLR3E RNA polymerase III subunit E 2 2
MIRT502294 GNG12 G protein subunit gamma 12 2 6
MIRT507600 DCTN4 dynactin subunit 4 2 4
MIRT510728 SON SON DNA binding protein 2 6
MIRT514065 KCNJ6 potassium voltage-gated channel subfamily J member 6 2 8
MIRT519718 ZNF512B zinc finger protein 512B 2 4
MIRT520890 STRN striatin 2 2
MIRT521760 PPIL1 peptidylprolyl isomerase like 1 2 6
MIRT526874 ERCC8 ERCC excision repair 8, CSA ubiquitin ligase complex subunit 2 2
MIRT530232 WSB2 WD repeat and SOCS box containing 2 2 2
MIRT532003 ACTR2 ARP2 actin related protein 2 homolog 2 2
MIRT533371 UBE2D4 ubiquitin conjugating enzyme E2 D4 (putative) 2 4
MIRT547106 PIGW phosphatidylinositol glycan anchor biosynthesis class W 2 2
MIRT548189 FOXA1 forkhead box A1 2 2
MIRT552935 VKORC1L1 vitamin K epoxide reductase complex subunit 1 like 1 2 2
MIRT560085 ZNF195 zinc finger protein 195 2 2
MIRT561726 PPP2CA protein phosphatase 2 catalytic subunit alpha 2 2
MIRT562713 ZNF415 zinc finger protein 415 2 2
MIRT562761 ZNF846 zinc finger protein 846 2 2
MIRT564159 ZNF117 zinc finger protein 117 2 2
MIRT565673 SETD5 SET domain containing 5 2 2
MIRT565718 SESN3 sestrin 3 2 2
MIRT566026 RFX1 regulatory factor X1 2 2
MIRT569048 ZNF655 zinc finger protein 655 2 2
MIRT570367 UBE2V1 ubiquitin conjugating enzyme E2 V1 2 2
MIRT570410 TMEM189-UBE2V1 TMEM189-UBE2V1 readthrough 2 2
MIRT570443 TMEM189 transmembrane protein 189 2 2
MIRT571738 RNF11 ring finger protein 11 2 2
MIRT575042 Tpgs2 tubulin polyglutamylase complex subunit 2 2 4
MIRT614330 ZDHHC22 zinc finger DHHC-type containing 22 2 2
MIRT617629 RAB3IP RAB3A interacting protein 2 2
MIRT621667 UBE4B ubiquitination factor E4B 2 2
MIRT639906 SRGAP2 SLIT-ROBO Rho GTPase activating protein 2 2 2
MIRT651436 XRCC5 X-ray repair cross complementing 5 2 2
MIRT683853 ZNF208 zinc finger protein 208 2 2
MIRT684841 TPGS2 tubulin polyglutamylase complex subunit 2 2 5
MIRT689347 ZNF83 zinc finger protein 83 2 2
MIRT692492 SPIN4 spindlin family member 4 2 2
MIRT695711 OLA1 Obg like ATPase 1 2 2
MIRT698219 TMEM248 transmembrane protein 248 2 2
MIRT711560 FAM20B FAM20B, glycosaminoglycan xylosylkinase 2 2
MIRT712867 TMEM67 transmembrane protein 67 2 2
MIRT722956 TSPAN1 tetraspanin 1 2 2
MIRT723622 SOBP sine oculis binding protein homolog 2 2
MIRT724176 ABCF2 ATP binding cassette subfamily F member 2 2 2
MIRT755363 LMBR1 limb development membrane protein 1 3 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-758 Hydroxycamptothecin (HCPT) NULL 97226 Microarray human Tenon's fibroblasts (HTFs) 24681041 2014 down-regulated
miR-758 Diethylstilbestrol approved 448537 Microarray mammosphere-derived epithelial cells (MDEC) 19549897 2009 up-regulated
miR-758 5-Fluorouracil approved 3385 Quantitative real-time PCR human squamous cell carcinoma cell line KYSE410 21743970 2011 up-regulated
miR-758 Reversine NULL 210332 Microarray C2C12 myoblast cells 24513286 2014 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-758 Ceritinib 57379345 NSC776422 approved resistant High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-mir-758 Fluorouracil 3385 NSC19893 approved resistant cell line (KYSE)
hsa-mir-758 Ceritinib 57379345 NSC776422 approved resistant cell line (H3122)
hsa-miR-758-5p Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-758-5p Osimertinib 71496458 NSC779217 approved sensitive cell line (HCC827)
hsa-miR-758-5p Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-758-5p Paclitaxel 36314 NSC125973 approved sensitive cell line (BAS)
hsa-miR-758-5p Doxorubicin 31703 NSC123127 approved sensitive cell line (BAS)
hsa-miR-758-5p Osimertinib 71496458 NSC779217 approved resistant cell line (H1975)
hsa-miR-758-5p Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (1500 ng/ml)
hsa-miR-758-5p Ceritinib 57379345 NSC776422 approved resistant cell line (H3122)

Error report submission