pre-miRNA Information
pre-miRNA hsa-mir-3622b   
Genomic Coordinates chr8: 27701673 - 27701767
Description Homo sapiens miR-3622b stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-3622b-3p
Sequence 58| UCACCUGAGCUCCCGUGCCUG |78
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1232922758 2 dbSNP
rs530939348 5 dbSNP
rs1439657384 10 dbSNP
rs1157333896 12 dbSNP
rs66683138 14 dbSNP
rs1044808718 15 dbSNP
rs946438979 19 dbSNP
rs551845360 21 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol HSPA6   
Synonyms HSP70B'
Description heat shock protein family A (Hsp70) member 6
Transcript NM_002155   
Expression
Putative miRNA Targets on HSPA6
3'UTR of HSPA6
(miRNA target sites are highlighted)
>HSPA6|NM_002155|3'UTR
   1 ATGGCCCTTCGTGATAAGTCAGCTGTGACTGTCAGGGCTATGCTATGGGCCTTCTAGACTGTCTTCTATGATCCTGCCCT
  81 TCAGAGATGAACTTTCCCTCCAAAGCTAGAACTTTCTTCCCAGGATAACTGAAGTCTTTTGACTTTTTGCGGGGAGGGCG
 161 GTTCATCCTCTTCTGCTTCAAATAAAAAGTCATTAATTTATTAAAACTTGTGTGGCACTTTAACATTGCTTTCACCTATA
 241 TTTTGTGTACTTTGTTACTTGCATGTATGAATTTTGTTATGTAAAATATAGTTATAGACCTAAATAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' guccGUGCCCUCGAGUCCACu 5'
              |::|  |  |||| || 
Target 5' ccttCGTGATAAGTCAGCTGt 3'
6 - 26 113.00 -7.50
2
miRNA  3' guCCGUGCCCUCGAGUccacu 5'
            || | || :|:|||     
Target 5' cgGGGAGGGCGGTTCAtcctc 3'
150 - 170 83.00 -13.23
3
miRNA  3' guccgugcccUCGAGUCCACu 5'
                    | ||:: ||| 
Target 5' aatttattaaAACTTGTGTGg 3'
195 - 215 83.00 -6.31
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN31486338 11 COSMIC
COSN30454765 36 COSMIC
COSN8646064 38 COSMIC
COSN15900125 101 COSMIC
COSN30513898 102 COSMIC
COSN20609487 135 COSMIC
COSN19749875 151 COSMIC
COSN31485761 189 COSMIC
COSN30113416 198 COSMIC
COSN31556743 210 COSMIC
COSN30126216 216 COSMIC
COSN30111185 230 COSMIC
COSN30124990 256 COSMIC
COSN30132489 268 COSMIC
COSN31530164 298 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1455611035 2 dbSNP
rs775967539 4 dbSNP
rs761412401 7 dbSNP
rs1415606586 10 dbSNP
rs766922629 11 dbSNP
rs1348667041 12 dbSNP
rs1465142681 13 dbSNP
rs760176269 15 dbSNP
rs1226521050 17 dbSNP
rs1048617819 25 dbSNP
rs1325833858 34 dbSNP
rs767967823 35 dbSNP
rs1217747245 37 dbSNP
rs1417511007 41 dbSNP
rs1161846335 42 dbSNP
rs1254000453 46 dbSNP
rs753144068 48 dbSNP
rs12562680 58 dbSNP
rs1474012981 62 dbSNP
rs1387311591 64 dbSNP
rs1181776250 71 dbSNP
rs888591388 76 dbSNP
rs1235857379 77 dbSNP
rs1200061206 79 dbSNP
rs60372173 92 dbSNP
rs60515741 94 dbSNP
rs879359847 98 dbSNP
rs1288776732 99 dbSNP
rs1445517104 109 dbSNP
rs1358250214 110 dbSNP
rs548995950 114 dbSNP
rs1037250594 116 dbSNP
rs1445985670 116 dbSNP
rs12073773 120 dbSNP
rs1333454941 123 dbSNP
rs1378571283 130 dbSNP
rs1349506698 134 dbSNP
rs1321906418 136 dbSNP
rs1407408701 138 dbSNP
rs1013958839 143 dbSNP
rs41299262 150 dbSNP
rs1042881 151 dbSNP
rs41299264 152 dbSNP
rs999881047 154 dbSNP
rs1290370004 155 dbSNP
rs1239255573 158 dbSNP
rs1339180096 160 dbSNP
rs1032822646 161 dbSNP
rs1239041211 165 dbSNP
rs1306388620 181 dbSNP
rs1352251782 184 dbSNP
rs955839684 191 dbSNP
rs1224201262 192 dbSNP
rs866607150 202 dbSNP
rs1440698704 208 dbSNP
rs1205298762 209 dbSNP
rs375069063 212 dbSNP
rs1461907993 216 dbSNP
rs1018902637 229 dbSNP
rs966347992 246 dbSNP
rs1423885198 248 dbSNP
rs10919261 251 dbSNP
rs12128362 263 dbSNP
rs1159277260 277 dbSNP
rs1247013697 280 dbSNP
rs1215613449 281 dbSNP
rs1425074755 282 dbSNP
rs1413306972 292 dbSNP
rs976808057 297 dbSNP
rs1217271077 298 dbSNP
rs1311634926 304 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions Hela
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1048187. RNA binding protein: AGO2. Condition:Hela_AGO2_CLIP_control ...

- Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al., 2013, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' guCCGUGCCCUCGAGUCcacu 5'
            | | || || |||||    
Target 5' --GACUCGUGACCUCAG---- 3'
1 - 15
Article - Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al.
- Cell, 2013
The induction of pluripotency or trans-differentiation of one cell type to another can be accomplished with cell-lineage-specific transcription factors. Here, we report that repression of a single RNA binding polypyrimidine-tract-binding (PTB) protein, which occurs during normal brain development via the action of miR-124, is sufficient to induce trans-differentiation of fibroblasts into functional neurons. Besides its traditional role in regulated splicing, we show that PTB has a previously undocumented function in the regulation of microRNA functions, suppressing or enhancing microRNA targeting by competitive binding on target mRNA or altering local RNA secondary structure. A key event during neuronal induction is the relief of PTB-mediated blockage of microRNA action on multiple components of the REST complex, thereby derepressing a large array of neuronal genes, including miR-124 and multiple neuronal-specific transcription factors, in nonneuronal cells. This converts a negative feedback loop to a positive one to elicit cellular reprogramming to the neuronal lineage.
LinkOut: [PMID: 23313552]
CLIP-seq Support 1 for dataset GSM1048187
Method / RBP HITS-CLIP / AGO2
Cell line / Condition Hela / Hela_AGO2_CLIP_control
Location of target site ENST00000309758.4 | 3UTR | GACUCGUGACCUCAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23313552 / GSE42701
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
THCA 0.045 0.46 -0.107 0.41 7 Click to see details
THCA 0.045 0.46 -0.107 0.41 7 Click to see details
THCA 0.045 0.46 -0.107 0.41 7 Click to see details
THCA 0.045 0.46 -0.107 0.41 7 Click to see details
THCA 0.045 0.46 -0.107 0.41 7 Click to see details
THCA 0.045 0.46 -0.107 0.41 7 Click to see details
102 hsa-miR-3622b-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT076713 NUFIP2 NUFIP2, FMR1 interacting protein 2 2 2
MIRT080212 PRKACB protein kinase cAMP-activated catalytic subunit beta 2 2
MIRT081398 GTPBP3 GTP binding protein 3, mitochondrial 2 2
MIRT107158 ZBTB43 zinc finger and BTB domain containing 43 2 2
MIRT254071 BACH1 BTB domain and CNC homolog 1 2 2
MIRT409790 FOXO3 forkhead box O3 2 2
MIRT448679 MAPK9 mitogen-activated protein kinase 9 2 2
MIRT450206 ABHD15 abhydrolase domain containing 15 2 2
MIRT486621 PDK3 pyruvate dehydrogenase kinase 3 2 2
MIRT489267 TTLL1 tubulin tyrosine ligase like 1 2 2
MIRT493117 MKNK2 MAP kinase interacting serine/threonine kinase 2 2 4
MIRT494570 BAK1 BCL2 antagonist/killer 1 2 2
MIRT497395 RALY RALY heterogeneous nuclear ribonucleoprotein 2 2
MIRT504900 CCDC86 coiled-coil domain containing 86 2 2
MIRT505192 USP46 ubiquitin specific peptidase 46 2 4
MIRT506522 MSANTD4 Myb/SANT DNA binding domain containing 4 with coiled-coils 2 6
MIRT508162 ABCC5 ATP binding cassette subfamily C member 5 2 8
MIRT508545 PARVG parvin gamma 2 4
MIRT509850 BIRC5 baculoviral IAP repeat containing 5 2 6
MIRT510088 PPWD1 peptidylprolyl isomerase domain and WD repeat containing 1 2 8
MIRT512094 CRK CRK proto-oncogene, adaptor protein 2 4
MIRT515381 RPL7 ribosomal protein L7 2 2
MIRT519176 SCO1 SCO1, cytochrome c oxidase assembly protein 2 2
MIRT519964 ZCCHC8 zinc finger CCHC-type containing 8 2 2
MIRT522292 NKAP NFKB activating protein 2 2
MIRT523160 HMGB2 high mobility group box 2 2 4
MIRT525314 FANCA Fanconi anemia complementation group A 2 2
MIRT528194 PLEKHM2 pleckstrin homology and RUN domain containing M2 2 2
MIRT532885 ZNF451 zinc finger protein 451 2 2
MIRT538209 CYR61 cysteine rich angiogenic inducer 61 2 2
MIRT539497 ACTN4 actinin alpha 4 2 2
MIRT554600 RRAGC Ras related GTP binding C 2 2
MIRT562444 DCTN6 dynactin subunit 6 2 2
MIRT562748 AOC3 amine oxidase, copper containing 3 2 2
MIRT565798 SEC14L5 SEC14 like lipid binding 5 2 2
MIRT565838 SCML2 Scm polycomb group protein like 2 2 2
MIRT566072 RCC2 regulator of chromosome condensation 2 2 2
MIRT566104 RBPJ recombination signal binding protein for immunoglobulin kappa J region 2 2
MIRT572407 MRPS14 mitochondrial ribosomal protein S14 2 2
MIRT576198 Vsig2 V-set and immunoglobulin domain containing 2 2 2
MIRT576313 Acbd7 acyl-Coenzyme A binding domain containing 7 2 2
MIRT576651 Mill2 MHC I like leukocyte 2 1 1
MIRT576859 Socs6 suppressor of cytokine signaling 6 2 2
MIRT606819 BICD2 BICD cargo adaptor 2 2 2
MIRT610738 NUDT16 nudix hydrolase 16 2 4
MIRT614798 RORA RAR related orphan receptor A 2 2
MIRT619006 NTMT1 N-terminal Xaa-Pro-Lys N-methyltransferase 1 2 2
MIRT619420 NOS1AP nitric oxide synthase 1 adaptor protein 2 2
MIRT620405 MYO1H myosin IH 2 2
MIRT624869 ABHD13 abhydrolase domain containing 13 2 2
MIRT633527 ZFP30 ZFP30 zinc finger protein 2 2
MIRT633927 DNAH9 dynein axonemal heavy chain 9 2 2
MIRT636611 CLIC5 chloride intracellular channel 5 2 2
MIRT640616 MIOX myo-inositol oxygenase 2 2
MIRT642605 C14orf180 chromosome 14 open reading frame 180 2 2
MIRT644624 SRSF2 serine and arginine rich splicing factor 2 2 2
MIRT655113 PHLDA3 pleckstrin homology like domain family A member 3 2 2
MIRT658976 DNAJB5 DnaJ heat shock protein family (Hsp40) member B5 2 4
MIRT661495 CHMP1B charged multivesicular body protein 1B 2 2
MIRT662996 TMEM59 transmembrane protein 59 2 2
MIRT663075 SFR1 SWI5 dependent homologous recombination repair protein 1 2 2
MIRT665405 WEE1 WEE1 G2 checkpoint kinase 2 2
MIRT666106 SSR1 signal sequence receptor subunit 1 2 2
MIRT669562 ALDOA aldolase, fructose-bisphosphate A 2 2
MIRT670070 ZNF783 zinc finger family member 783 2 2
MIRT671192 ZNF891 zinc finger protein 891 2 2
MIRT675361 KLHL26 kelch like family member 26 2 2
MIRT678857 LINC00598 long intergenic non-protein coding RNA 598 2 2
MIRT679443 C19orf52 translocase of inner mitochondrial membrane 29 2 2
MIRT684163 ALDH1B1 aldehyde dehydrogenase 1 family member B1 2 2
MIRT684543 ZNF460 zinc finger protein 460 2 2
MIRT684674 SLC2A11 solute carrier family 2 member 11 2 2
MIRT684971 MINOS1 mitochondrial inner membrane organizing system 1 2 2
MIRT686006 NEK4 NIMA related kinase 4 2 2
MIRT687758 KIAA1328 KIAA1328 2 2
MIRT688961 ATXN3 ataxin 3 2 2
MIRT689409 UQCR11 ubiquinol-cytochrome c reductase, complex III subunit XI 2 2
MIRT690001 MMP17 matrix metallopeptidase 17 2 2
MIRT690165 ELP3 elongator acetyltransferase complex subunit 3 2 2
MIRT690202 C5orf45 MRN complex interacting protein 2 2
MIRT690477 ZNF33A zinc finger protein 33A 2 2
MIRT690566 MICA MHC class I polypeptide-related sequence A 2 2
MIRT692160 C10orf111 chromosome 10 open reading frame 111 2 2
MIRT693430 PLGLB2 plasminogen-like B2 2 2
MIRT693547 ZNF708 zinc finger protein 708 2 2
MIRT693697 PLGLB1 plasminogen-like B1 2 2
MIRT695006 HSPA6 heat shock protein family A (Hsp70) member 6 2 2
MIRT698525 TFRC transferrin receptor 2 2
MIRT699574 SIKE1 suppressor of IKBKE 1 2 2
MIRT703446 FYTTD1 forty-two-three domain containing 1 2 2
MIRT704397 CTSS cathepsin S 2 2
MIRT704485 CPT1A carnitine palmitoyltransferase 1A 2 2
MIRT709822 STPG1 sperm tail PG-rich repeat containing 1 2 2
MIRT710859 COQ7 coenzyme Q7, hydroxylase 2 2
MIRT711893 INSIG2 insulin induced gene 2 2 2
MIRT713796 CPLX2 complexin 2 2 2
MIRT718800 C1GALT1C1 C1GALT1 specific chaperone 1 2 2
MIRT719501 SEC24B SEC24 homolog B, COPII coat complex component 2 2
MIRT719722 PDE6B phosphodiesterase 6B 2 2
MIRT722204 URM1 ubiquitin related modifier 1 2 2
MIRT723523 CLPTM1L CLPTM1 like 2 2
MIRT725471 GRAP2 GRB2-related adaptor protein 2 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-3622b-3p Platinum 23939 sensitive tissue
hsa-miR-3622b-3p Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-3622b-3p Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (1500 ng/ml)
hsa-miR-3622b-3p Paclitaxel/Docetaxel/Vinorelbine/Doxorubicin/Etoposide resistant cell line (Bads-200)

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