pre-miRNA Information
pre-miRNA hsa-mir-4481   
Genomic Coordinates chr10: 12653138 - 12653197
Description Homo sapiens miR-4481 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4481
Sequence 1| GGAGUGGGCUGGUGGUU |17
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1299710636 5 dbSNP
rs1433739601 12 dbSNP
rs1266754238 14 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol ASB16   
Synonyms -
Description ankyrin repeat and SOCS box containing 16
Transcript NM_080863   
Expression
Putative miRNA Targets on ASB16
3'UTR of ASB16
(miRNA target sites are highlighted)
>ASB16|NM_080863|3'UTR
   1 ACCCCATGTCAGGCTGTCCCATGGTGTGTTCTGCCCCTCCCACCTGTCCCCGCCTCCAACTGCGGAGGACCAGTTCCTGG
  81 CCCTCTTTTCTTTTCTTTTTGAGACCTAGTCTCACTCTGTTGCCCAGGCTGGAGTGCAGTGGCGCTATCTCGGCTCACTG
 161 CAACTTCTACCACCTAGGTTCAAGCGATTCTTGTGCCCCAAACTTCCGAGTAGCTGGGACTACAGGCATGAGTCACCACA
 241 CCTGGCTGATTTTGTATTTTTAGTAGAGACAGGGTCTCACCATGTTGGCCAGGCTGGTCTCACACTGACCTCAGGTGATC
 321 CACCCGCCTTGGCCTCCCAAAGTGTTGGGATTACAGGCATGAGCCACTGCGCCTGGTTGCCTGCCCCTCTTTTCTGTACT
 401 CCACGTGCTGTCCTGGTCCACCTCACTCCTCCATGGGCTTCTTGAGACACCTGCTGACCTCTAGCCGGACCTGAGCTTCA
 481 GACCCTCTCTCCAGCAGGAAGGGCTGCAGACTCGGCCTGTTCCCAGACTCGGCCTTCACCTCCCTTCTCCTCCTGTGTTT
 561 AAGTGGATGGCCCCTCTATCCATCCAGGTCCCATGCCAAGAACCAGTGATTCTAGTGGCCCACCACCTCCCTCTCCTGCC
 641 ACCCTTATACCTGTTCAGCCTCCTAAAGAGGGACAAACTCTGTCTCTGCGTGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' uugguGGUCGGGUGAGg 5'
               ||| |:||||| 
Target 5' ctggtCCACCTCACTCc 3'
413 - 429 136.00 -18.10
2
miRNA  3' uuggUGG-UC-GGGUGAGg 5'
              ||| || |:||||| 
Target 5' tgagACCTAGTCTCACTCt 3'
100 - 118 133.00 -14.50
3
miRNA  3' uuGGUGGU--CGGGUGAGg 5'
            ||||||   ||| ||| 
Target 5' gcCCACCACCTCCCTCTCc 3'
618 - 636 125.00 -21.70
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN22337415 11 COSMIC
COSN30137645 38 COSMIC
COSN27046342 64 COSMIC
COSN30172244 77 COSMIC
COSN15279431 385 COSMIC
COSN22372207 652 COSMIC
rs12937692 673 GWAS
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs753116195 2 dbSNP
rs753851123 2 dbSNP
rs754500086 4 dbSNP
rs1410870503 8 dbSNP
rs778609170 9 dbSNP
rs1371882928 13 dbSNP
rs889871035 17 dbSNP
rs747737024 18 dbSNP
rs375748291 20 dbSNP
rs1358924079 21 dbSNP
rs781409238 23 dbSNP
rs1179582384 25 dbSNP
rs746181286 27 dbSNP
rs369351726 34 dbSNP
rs201560232 35 dbSNP
rs763489064 37 dbSNP
rs768697582 41 dbSNP
rs200826931 42 dbSNP
rs1384090672 43 dbSNP
rs1442351143 44 dbSNP
rs1237276561 50 dbSNP
rs761936185 51 dbSNP
rs757065458 52 dbSNP
rs369725603 53 dbSNP
rs1380801742 57 dbSNP
rs1228213216 59 dbSNP
rs533182118 64 dbSNP
rs1180396577 65 dbSNP
rs909214511 72 dbSNP
rs941890890 77 dbSNP
rs990981243 80 dbSNP
rs1473231030 84 dbSNP
rs1398158336 91 dbSNP
rs1165122298 94 dbSNP
rs147737256 96 dbSNP
rs916718970 101 dbSNP
rs1382797976 106 dbSNP
rs1164946952 111 dbSNP
rs967805763 118 dbSNP
rs1379685607 121 dbSNP
rs551504997 122 dbSNP
rs1439389840 124 dbSNP
rs1251015250 127 dbSNP
rs560598270 128 dbSNP
rs1196556527 129 dbSNP
rs1286178474 133 dbSNP
rs527787722 145 dbSNP
rs549158059 146 dbSNP
rs746053107 148 dbSNP
rs567509207 152 dbSNP
rs1221002091 153 dbSNP
rs1237296283 166 dbSNP
rs931879773 174 dbSNP
rs531622744 175 dbSNP
rs1203501527 176 dbSNP
rs770060547 177 dbSNP
rs1363333369 179 dbSNP
rs887582156 186 dbSNP
rs1359795313 187 dbSNP
rs1005608899 189 dbSNP
rs1038398602 196 dbSNP
rs550130290 202 dbSNP
rs1471134357 204 dbSNP
rs1189871934 208 dbSNP
rs775429973 209 dbSNP
rs144711933 211 dbSNP
rs1052661146 214 dbSNP
rs896741426 218 dbSNP
rs749327334 219 dbSNP
rs1023829948 222 dbSNP
rs968192566 225 dbSNP
rs1169243490 228 dbSNP
rs1283689395 229 dbSNP
rs769060341 229 dbSNP
rs538596484 233 dbSNP
rs956438053 234 dbSNP
rs1390739829 238 dbSNP
rs1223346988 242 dbSNP
rs1343237303 255 dbSNP
rs1284853949 268 dbSNP
rs976157351 270 dbSNP
rs1007654432 272 dbSNP
rs1402309792 280 dbSNP
rs1385665870 293 dbSNP
rs1016244346 301 dbSNP
rs553500278 306 dbSNP
rs1427423879 307 dbSNP
rs963390618 316 dbSNP
rs1480899391 317 dbSNP
rs1241531211 318 dbSNP
rs147833434 326 dbSNP
rs536007274 339 dbSNP
rs1263115592 340 dbSNP
rs1203406176 352 dbSNP
rs1321901629 357 dbSNP
rs960143502 361 dbSNP
rs909073576 364 dbSNP
rs543766636 365 dbSNP
rs948470054 366 dbSNP
rs774874599 371 dbSNP
rs116243986 372 dbSNP
rs1336046194 373 dbSNP
rs573927298 378 dbSNP
rs1450397860 384 dbSNP
rs745777987 387 dbSNP
rs544481546 405 dbSNP
rs189533106 406 dbSNP
rs1419157327 409 dbSNP
rs577930824 414 dbSNP
rs930035465 435 dbSNP
rs1183471696 438 dbSNP
rs536872607 438 dbSNP
rs1226819834 441 dbSNP
rs1251169752 443 dbSNP
rs998177289 451 dbSNP
rs896464399 460 dbSNP
rs1254359375 462 dbSNP
rs1313764069 463 dbSNP
rs73316154 467 dbSNP
rs759173914 468 dbSNP
rs1441281011 472 dbSNP
rs1354134476 476 dbSNP
rs1327146059 478 dbSNP
rs560332064 484 dbSNP
rs1390805811 490 dbSNP
rs1163016808 491 dbSNP
rs963360088 514 dbSNP
rs1001032550 515 dbSNP
rs1031130293 523 dbSNP
rs956668334 527 dbSNP
rs764793258 529 dbSNP
rs527777634 531 dbSNP
rs752152358 532 dbSNP
rs986038893 535 dbSNP
rs909123959 537 dbSNP
rs1372368274 538 dbSNP
rs959950107 540 dbSNP
rs542789594 544 dbSNP
rs1219700630 547 dbSNP
rs561043534 556 dbSNP
rs1174064322 564 dbSNP
rs1315700502 565 dbSNP
rs1222961701 567 dbSNP
rs941905088 577 dbSNP
rs531508777 586 dbSNP
rs1444555895 587 dbSNP
rs1371481664 588 dbSNP
rs993167999 591 dbSNP
rs1422875028 595 dbSNP
rs1451432451 597 dbSNP
rs919071125 604 dbSNP
rs550018602 608 dbSNP
rs1320711634 614 dbSNP
rs193201576 621 dbSNP
rs969890777 624 dbSNP
rs1472710542 626 dbSNP
rs1366733609 628 dbSNP
rs1196616949 629 dbSNP
rs1432177248 640 dbSNP
rs930072505 644 dbSNP
rs1291905055 649 dbSNP
rs1214921674 651 dbSNP
rs1315064703 653 dbSNP
rs978470729 657 dbSNP
rs1295512460 659 dbSNP
rs1224191364 660 dbSNP
rs1351320445 661 dbSNP
rs925740905 663 dbSNP
rs796310885 665 dbSNP
rs896519366 672 dbSNP
rs922267240 672 dbSNP
rs12937692 673 dbSNP
rs551598836 678 dbSNP
rs1161715912 687 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions Hela
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1048187. RNA binding protein: AGO2. Condition:Hela_AGO2_CLIP_control ...

- Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al., 2013, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' uugGUGGU-CGGGUGAgg 5'
             ::|:: |:|||||  
Target 5' augUGCUGUGUCCACU-- 3'
3 - 18
Article - Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al.
- Cell, 2013
The induction of pluripotency or trans-differentiation of one cell type to another can be accomplished with cell-lineage-specific transcription factors. Here, we report that repression of a single RNA binding polypyrimidine-tract-binding (PTB) protein, which occurs during normal brain development via the action of miR-124, is sufficient to induce trans-differentiation of fibroblasts into functional neurons. Besides its traditional role in regulated splicing, we show that PTB has a previously undocumented function in the regulation of microRNA functions, suppressing or enhancing microRNA targeting by competitive binding on target mRNA or altering local RNA secondary structure. A key event during neuronal induction is the relief of PTB-mediated blockage of microRNA action on multiple components of the REST complex, thereby derepressing a large array of neuronal genes, including miR-124 and multiple neuronal-specific transcription factors, in nonneuronal cells. This converts a negative feedback loop to a positive one to elicit cellular reprogramming to the neuronal lineage.
LinkOut: [PMID: 23313552]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions Cardiac Tissues
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM2202478. RNA binding protein: AGO2. Condition:S3_LV_36yo_Male_AGO2_bound_RNA ...

- Spengler RM; Zhang X; Cheng C; McLendon JM; et al., 2016, Nucleic acids research.

Article - Spengler RM; Zhang X; Cheng C; McLendon JM; et al.
- Nucleic acids research, 2016
MicroRNAs (miRs) have emerged as key biological effectors in human health and disease. These small noncoding RNAs are incorporated into Argonaute (Ago) proteins, where they direct post-transcriptional gene silencing via base-pairing with target transcripts. Although miRs have become intriguing biological entities and attractive therapeutic targets, the translational impacts of miR research remain limited by a paucity of empirical miR targeting data, particularly in human primary tissues. Here, to improve our understanding of the diverse roles miRs play in cardiovascular function and disease, we applied high-throughput methods to globally profile miR:target interactions in human heart tissues. We deciphered Ago2:RNA interactions using crosslinking immunoprecipitation coupled with high-throughput sequencing (HITS-CLIP) to generate the first transcriptome-wide map of miR targeting events in human myocardium, detecting 4000 cardiac Ago2 binding sites across >2200 target transcripts. Our initial exploration of this interactome revealed an abundance of miR target sites in gene coding regions, including several sites pointing to new miR-29 functions in regulating cardiomyocyte calcium, growth and metabolism. Also, we uncovered several clinically-relevant interactions involving common genetic variants that alter miR targeting events in cardiomyopathy-associated genes. Overall, these data provide a critical resource for bolstering translational miR research in heart, and likely beyond.
LinkOut: [PMID: 27418678]
CLIP-seq Support 1 for dataset GSM1048187
Method / RBP HITS-CLIP / AGO2
Cell line / Condition Hela / Hela_AGO2_CLIP_control
Location of target site ENST00000293414.1 | 3UTR | ACAUGUGCUGUGUCCACU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23313552 / GSE42701
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
84 hsa-miR-4481 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT100715 TJAP1 tight junction associated protein 1 2 2
MIRT183589 ZC3H11A zinc finger CCCH-type containing 11A 2 2
MIRT338033 DAZAP2 DAZ associated protein 2 2 4
MIRT395796 SPCS3 signal peptidase complex subunit 3 2 2
MIRT443947 LRIT3 leucine rich repeat, Ig-like and transmembrane domains 3 2 2
MIRT450580 HIST1H2BG histone cluster 1 H2B family member g 2 6
MIRT451617 MEIS3P1 Meis homeobox 3 pseudogene 1 2 2
MIRT452331 EIF5AL1 eukaryotic translation initiation factor 5A-like 1 2 2
MIRT453279 EFTUD2 elongation factor Tu GTP binding domain containing 2 2 2
MIRT455212 GNL1 G protein nucleolar 1 (putative) 2 2
MIRT455470 LYPLA2 lysophospholipase II 2 2
MIRT456503 PFKFB2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 2 2
MIRT456687 LDB1 LIM domain binding 1 2 2
MIRT456915 DDA1 DET1 and DDB1 associated 1 2 2
MIRT457603 IDS iduronate 2-sulfatase 2 2
MIRT457848 RNASEH2B ribonuclease H2 subunit B 2 4
MIRT458455 RPRM reprimo, TP53 dependent G2 arrest mediator homolog 2 2
MIRT460179 UNK unkempt family zinc finger 2 6
MIRT461465 SLC19A3 solute carrier family 19 member 3 2 2
MIRT464739 UBE2Q1 ubiquitin conjugating enzyme E2 Q1 2 2
MIRT465279 TRIM28 tripartite motif containing 28 2 2
MIRT468450 SETD1B SET domain containing 1B 2 2
MIRT468620 SUMO1 small ubiquitin-like modifier 1 2 6
MIRT469152 RNF121 ring finger protein 121 2 2
MIRT470073 PTGES2 prostaglandin E synthase 2 2 2
MIRT470172 PSMD11 proteasome 26S subunit, non-ATPase 11 2 4
MIRT473156 MLLT1 MLLT1, super elongation complex subunit 2 2
MIRT474308 LAMC1 laminin subunit gamma 1 2 2
MIRT474782 KIAA0895L KIAA0895 like 2 2
MIRT476484 GATAD2A GATA zinc finger domain containing 2A 2 2
MIRT477613 EFNA3 ephrin A3 2 2
MIRT479525 CDCA4 cell division cycle associated 4 2 2
MIRT479973 CARD10 caspase recruitment domain family member 10 2 2
MIRT480410 C19orf47 chromosome 19 open reading frame 47 2 2
MIRT480426 C17orf85 nuclear cap binding subunit 3 2 2
MIRT483573 SYT2 synaptotagmin 2 2 2
MIRT483664 QSOX2 quiescin sulfhydryl oxidase 2 2 4
MIRT484532 POLD3 DNA polymerase delta 3, accessory subunit 2 2
MIRT484616 SIX3 SIX homeobox 3 2 6
MIRT485897 ZFP36 ZFP36 ring finger protein 2 2
MIRT486505 MYH11 myosin heavy chain 11 2 2
MIRT487505 GRK5 G protein-coupled receptor kinase 5 2 2
MIRT488852 UBTF upstream binding transcription factor, RNA polymerase I 2 2
MIRT489464 MSC musculin 2 2
MIRT491170 LRP3 LDL receptor related protein 3 2 2
MIRT496627 TMEM67 transmembrane protein 67 2 2
MIRT497616 ANG angiogenin 2 2
MIRT497766 KIAA0895 KIAA0895 2 2
MIRT499680 MRE11A MRE11 homolog, double strand break repair nuclease 2 6
MIRT499774 SLC29A2 solute carrier family 29 member 2 2 2
MIRT501745 NSD1 nuclear receptor binding SET domain protein 1 2 2
MIRT504995 ZNF652 zinc finger protein 652 2 2
MIRT511664 HIST1H3C histone cluster 1 H3 family member c 2 2
MIRT511690 HIST1H2BO histone cluster 1 H2B family member o 2 4
MIRT511703 HIST1H2BL histone cluster 1 H2B family member l 2 4
MIRT511734 HIST1H2BE histone cluster 1 H2B family member e 2 8
MIRT512858 TBC1D13 TBC1 domain family member 13 2 2
MIRT513444 EMP1 epithelial membrane protein 1 2 6
MIRT515681 TFPI tissue factor pathway inhibitor 2 2
MIRT523529 GLUL glutamate-ammonia ligase 2 2
MIRT525546 PHB2 prohibitin 2 2 4
MIRT526209 SNX24 sorting nexin 24 2 2
MIRT531554 SRD5A1 steroid 5 alpha-reductase 1 2 2
MIRT533764 TMEM135 transmembrane protein 135 2 2
MIRT545582 SNRPA1 small nuclear ribonucleoprotein polypeptide A' 2 2
MIRT552434 ZNF460 zinc finger protein 460 2 2
MIRT561159 BCL2L12 BCL2 like 12 2 2
MIRT562345 EXOSC2 exosome component 2 2 2
MIRT570659 KDM6B lysine demethylase 6B 2 2
MIRT571076 TCHHL1 trichohyalin like 1 2 2
MIRT571330 TPCN2 two pore segment channel 2 2 2
MIRT571603 TOB2 transducer of ERBB2, 2 2 2
MIRT573013 RPP25 ribonuclease P and MRP subunit p25 2 2
MIRT609041 EP300 E1A binding protein p300 2 2
MIRT613398 DNAH17 dynein axonemal heavy chain 17 2 2
MIRT635589 TTC9C tetratricopeptide repeat domain 9C 2 2
MIRT661118 FPR1 formyl peptide receptor 1 2 2
MIRT690103 PNMA2 paraneoplastic Ma antigen 2 2 2
MIRT694972 PLAC8 placenta specific 8 2 2
MIRT695515 ALPI alkaline phosphatase, intestinal 2 2
MIRT695578 ASB16 ankyrin repeat and SOCS box containing 16 2 2
MIRT699570 SIT1 signaling threshold regulating transmembrane adaptor 1 2 2
MIRT701991 MIER3 MIER family member 3 2 2
MIRT725464 GRAP2 GRB2-related adaptor protein 2 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-4 Dexamethasone approved 5743 Microarray primary rat thymocytes 20847043 2010 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-4481 Platinum 23939 sensitive High Ovarian Cancer tissue
hsa-mir-4481 Androstenedione 6128 NSC9563 sensitive cell line (MCF-7)
hsa-mir-4481 Tamoxifen 2733525 NSC180973 approved resistant cell line (MCF7)
hsa-mir-4481 Cisplatin 5460033 NSC119875 approved resistant cell line (BxPC3)
hsa-miR-4481 Cisplatin 5460033 NSC119875 approved resistant cell line (CAL-27) (cytosolic RNA)
hsa-miR-4481 Osimertinib 71496458 NSC779217 approved sensitive cell line (H1975)
hsa-miR-4481 Neoadjuvant chemotherapy sensitive tissue (breast cancer)
hsa-miR-4481 Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (100 ng/ml)
hsa-miR-4481 Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (1500 ng/ml)

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