pre-miRNA Information
pre-miRNA hsa-mir-6132   
Genomic Coordinates chr7: 117020211 - 117020319
Description Homo sapiens miR-6132 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-6132
Sequence 21| AGCAGGGCUGGGGAUUGCA |39
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1272646274 4 dbSNP
rs1211969145 8 dbSNP
rs1310278496 11 dbSNP
rs1044115534 13 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol NUP35   
Synonyms MP-44, MP44, NP44, NUP53
Description nucleoporin 35
Transcript NM_138285   
Expression
Putative miRNA Targets on NUP35
3'UTR of NUP35
(miRNA target sites are highlighted)
>NUP35|NM_138285|3'UTR
   1 TAGAACACCAAGAAGGAGGTTGCTACACTAAAACAGAGTTAGCAGAGTGCTGCTGGTTCCTTCGGTTAGTTATATAACTG
  81 TTCCTGCAGTATTGGATAGCTATCTCATACTTCTTTTAGAAAGAAGCCTTTTTCATTAAGGATACAACCTATTTGTAGCT
 161 CGCACTTTAAAAGATGCTTGAGATACATTTTAAAGAAAACTAAAAATCCCTGTAAATAGGATTTTGTGCTTTCTGTAACA
 241 GTGCATGCTTCAGCACAGAAAACTCAGCATTGATTATTGTAAATTAAATAACTGAAATTGTGGTGAGACGTCATAGTCTT
 321 CATGAGAACGTGGGGGTGAATTTCATGAAGGGGAACTATAGTTATTTCTACCGACACAAATATTATAATTAGCAATTTGA
 401 ATTATGGTCTTTTAATTTAGATAGTATTTAATATTTTAATTATCCTTGTTTGTATATGTCCTGTCACAGAGTGTCCTCTT
 481 GGTGTATTCTAAAACGAGCATTCTTTTAAAAAACCTAAAGTTTCTTGATAATAAACATTGTCAATGATATGTG
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' acguuagggguCGGGACGa 5'
                     |:||||: 
Target 5' tgtttgtatatGTCCTGTc 3'
447 - 465 108.00 -7.70
2
miRNA  3' acguuaggggucGGGACGa 5'
                      :||||| 
Target 5' ttatataactgtTCCTGCa 3'
70 - 88 104.00 -9.10
3
miRNA  3' acguuaggggucGGGACGa 5'
                      |||||: 
Target 5' aaaactaaaaatCCCTGTa 3'
196 - 214 104.00 -7.50
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN30456805 10 COSMIC
COSN20121741 38 COSMIC
COSN30473700 51 COSMIC
COSN30117268 85 COSMIC
COSN31582962 124 COSMIC
COSN31610760 177 COSMIC
COSN31558241 206 COSMIC
COSN22547835 259 COSMIC
COSN20090687 384 COSMIC
COSN28158758 405 COSMIC
COSN21067206 472 COSMIC
COSN9120420 497 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs773115663 4 dbSNP
rs1249767936 7 dbSNP
rs762537479 9 dbSNP
rs751039361 12 dbSNP
rs759033297 13 dbSNP
rs202005464 14 dbSNP
rs74849582 17 dbSNP
rs79259445 18 dbSNP
rs1404791252 19 dbSNP
rs192556307 26 dbSNP
rs750745259 28 dbSNP
rs377536338 29 dbSNP
rs1259585483 32 dbSNP
rs1396985013 32 dbSNP
rs1385962929 33 dbSNP
rs1297308935 42 dbSNP
rs1298798996 43 dbSNP
rs1179883364 44 dbSNP
rs1332430349 45 dbSNP
rs1383163689 47 dbSNP
rs547826649 49 dbSNP
rs370967150 52 dbSNP
rs755149474 53 dbSNP
rs1232804634 57 dbSNP
rs1335518215 65 dbSNP
rs571989835 69 dbSNP
rs1031805426 77 dbSNP
rs1335558078 82 dbSNP
rs1288950332 97 dbSNP
rs1386446631 101 dbSNP
rs960518640 103 dbSNP
rs1324781939 104 dbSNP
rs1039335592 105 dbSNP
rs1324773875 107 dbSNP
rs183519395 108 dbSNP
rs749709044 110 dbSNP
rs1156878310 111 dbSNP
rs1306726299 111 dbSNP
rs1316284667 115 dbSNP
rs1239618952 117 dbSNP
rs774106282 118 dbSNP
rs1183799409 123 dbSNP
rs1265586670 129 dbSNP
rs557352324 129 dbSNP
rs56987972 130 dbSNP
rs769844038 131 dbSNP
rs1275066002 138 dbSNP
rs536247437 141 dbSNP
rs555424403 143 dbSNP
rs999649704 149 dbSNP
rs1253850819 150 dbSNP
rs775873597 154 dbSNP
rs1329676589 161 dbSNP
rs553435481 162 dbSNP
rs1441118562 163 dbSNP
rs896109684 180 dbSNP
rs572959374 183 dbSNP
rs1396039774 206 dbSNP
rs57022552 210 dbSNP
rs1023152841 218 dbSNP
rs564711138 219 dbSNP
rs1036697862 223 dbSNP
rs575073471 227 dbSNP
rs1385642896 228 dbSNP
rs544135601 240 dbSNP
rs748934668 240 dbSNP
rs764301401 241 dbSNP
rs1476937621 243 dbSNP
rs1193255682 246 dbSNP
rs1454822038 262 dbSNP
rs1255118579 267 dbSNP
rs1206969028 270 dbSNP
rs970239634 284 dbSNP
rs1285620044 292 dbSNP
rs561019003 293 dbSNP
rs919090997 310 dbSNP
rs973534407 311 dbSNP
rs1027744342 312 dbSNP
rs1279978214 314 dbSNP
rs3208309 322 dbSNP
rs1372803421 324 dbSNP
rs983920515 330 dbSNP
rs1161880097 331 dbSNP
rs1326463850 333 dbSNP
rs911911719 334 dbSNP
rs1412630208 336 dbSNP
rs1374629008 346 dbSNP
rs933190736 347 dbSNP
rs966427950 353 dbSNP
rs1365089170 354 dbSNP
rs756764023 354 dbSNP
rs1397170909 359 dbSNP
rs1184965746 362 dbSNP
rs1475142104 366 dbSNP
rs974681495 371 dbSNP
rs1195968292 373 dbSNP
rs1051780198 374 dbSNP
rs1163847476 380 dbSNP
rs889228736 380 dbSNP
rs921846345 383 dbSNP
rs773254221 384 dbSNP
rs72896520 386 dbSNP
rs1213577972 387 dbSNP
rs923732898 391 dbSNP
rs935107086 405 dbSNP
rs766185234 410 dbSNP
rs745814006 411 dbSNP
rs1056602688 420 dbSNP
rs896174962 422 dbSNP
rs1445166594 426 dbSNP
rs999108890 434 dbSNP
rs1012320801 449 dbSNP
rs1416429007 452 dbSNP
rs1368423690 455 dbSNP
rs771941726 457 dbSNP
rs1409542962 464 dbSNP
rs775397957 468 dbSNP
rs1169788155 474 dbSNP
rs1014458687 477 dbSNP
rs1257485706 496 dbSNP
rs546233892 497 dbSNP
rs1486871365 503 dbSNP
rs1023151380 507 dbSNP
rs1203383202 517 dbSNP
rs560204710 518 dbSNP
rs980517713 525 dbSNP
rs1226166305 527 dbSNP
rs1333487315 530 dbSNP
rs908846058 536 dbSNP
rs963411732 537 dbSNP
rs1348411088 538 dbSNP
rs995009999 547 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions Hela
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1048187. RNA binding protein: AGO2. Condition:Hela_AGO2_CLIP_control ...

- Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al., 2013, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' acGUUAGGGGU-CGGGACGa 5'
            |:|||:: :  |||||| 
Target 5' ugCGAUCUUGGCUCCCUGC- 3'
11 - 29
Article - Xue Y; Ouyang K; Huang J; Zhou Y; Ouyang H; et al.
- Cell, 2013
The induction of pluripotency or trans-differentiation of one cell type to another can be accomplished with cell-lineage-specific transcription factors. Here, we report that repression of a single RNA binding polypyrimidine-tract-binding (PTB) protein, which occurs during normal brain development via the action of miR-124, is sufficient to induce trans-differentiation of fibroblasts into functional neurons. Besides its traditional role in regulated splicing, we show that PTB has a previously undocumented function in the regulation of microRNA functions, suppressing or enhancing microRNA targeting by competitive binding on target mRNA or altering local RNA secondary structure. A key event during neuronal induction is the relief of PTB-mediated blockage of microRNA action on multiple components of the REST complex, thereby derepressing a large array of neuronal genes, including miR-124 and multiple neuronal-specific transcription factors, in nonneuronal cells. This converts a negative feedback loop to a positive one to elicit cellular reprogramming to the neuronal lineage.
LinkOut: [PMID: 23313552]
CLIP-seq Support 1 for dataset GSM1048187
Method / RBP HITS-CLIP / AGO2
Cell line / Condition Hela / Hela_AGO2_CLIP_control
Location of target site ENST00000295119.4 | 3UTR | AGUGCAGUGGUGCGAUCUUGGCUCCCUGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23313552 / GSE42701
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
82 hsa-miR-6132 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT067294 NECAP1 NECAP endocytosis associated 1 2 10
MIRT100110 ABT1 activator of basal transcription 1 2 8
MIRT358583 CANX calnexin 2 2
MIRT445247 SEMA5A semaphorin 5A 2 2
MIRT445764 CCND3 cyclin D3 2 2
MIRT452388 LY6E lymphocyte antigen 6 family member E 2 4
MIRT452829 FAM131B family with sequence similarity 131 member B 2 2
MIRT453450 GLG1 golgi glycoprotein 1 2 2
MIRT455435 ID3 inhibitor of DNA binding 3, HLH protein 2 2
MIRT460629 IGFBP4 insulin like growth factor binding protein 4 2 2
MIRT461607 DPH2 DPH2 homolog 2 2
MIRT461989 PACSIN1 protein kinase C and casein kinase substrate in neurons 1 2 2
MIRT464258 VCL vinculin 2 2
MIRT465713 TNFAIP1 TNF alpha induced protein 1 2 2
MIRT466475 TECPR2 tectonin beta-propeller repeat containing 2 2 7
MIRT467119 SRGAP1 SLIT-ROBO Rho GTPase activating protein 1 2 4
MIRT468299 SFT2D2 SFT2 domain containing 2 2 2
MIRT469696 RAB5B RAB5B, member RAS oncogene family 2 8
MIRT469904 PTRF caveolae associated protein 1 2 2
MIRT470015 PTPLB 3-hydroxyacyl-CoA dehydratase 2 1 1
MIRT471385 PDPR pyruvate dehydrogenase phosphatase regulatory subunit 2 2
MIRT471409 PDP2 pyruvate dehyrogenase phosphatase catalytic subunit 2 2 2
MIRT471719 OTUB1 OTU deubiquitinase, ubiquitin aldehyde binding 1 2 2
MIRT473608 MARK2 microtubule affinity regulating kinase 2 2 2
MIRT476328 GLTSCR1L BRD4 interacting chromatin remodeling complex associated protein like 2 2
MIRT479448 CDK6 cyclin dependent kinase 6 2 2
MIRT482032 AMER1 APC membrane recruitment protein 1 2 2
MIRT482360 AGO2 argonaute 2, RISC catalytic component 2 2
MIRT484661 HOXD3 homeobox D3 2 4
MIRT486828 NDOR1 NADPH dependent diflavin oxidoreductase 1 2 2
MIRT487069 CLASP1 cytoplasmic linker associated protein 1 2 4
MIRT487196 NFASC neurofascin 2 4
MIRT487448 TFAP2B transcription factor AP-2 beta 2 4
MIRT487517 GXYLT2 glucoside xylosyltransferase 2 2 2
MIRT487640 BRSK2 BR serine/threonine kinase 2 2 4
MIRT487755 SKI SKI proto-oncogene 2 4
MIRT489944 CPLX1 complexin 1 2 2
MIRT491101 MSI1 musashi RNA binding protein 1 2 4
MIRT491181 LAMA5 laminin subunit alpha 5 2 2
MIRT492355 SEMA7A semaphorin 7A (John Milton Hagen blood group) 2 2
MIRT493918 FAM127B retrotransposon Gag like 8A 2 4
MIRT493932 FAM127A retrotransposon Gag like 8C 2 4
MIRT494679 ARID3A AT-rich interaction domain 3A 2 2
MIRT494814 AKAP11 A-kinase anchoring protein 11 2 2
MIRT494835 ADCY9 adenylate cyclase 9 2 2
MIRT495455 PNMAL2 paraneoplastic Ma antigen family member 8B 2 2
MIRT496962 MAP1LC3B microtubule associated protein 1 light chain 3 beta 2 2
MIRT526228 MTRNR2L5 MT-RNR2-like 5 2 2
MIRT531028 TDGF1P3 teratocarcinoma-derived growth factor 1 pseudogene 3 2 2
MIRT557110 HOXA3 homeobox A3 2 2
MIRT560514 POGK pogo transposable element derived with KRAB domain 2 2
MIRT567753 DLC1 DLC1 Rho GTPase activating protein 2 2
MIRT569608 TRIM29 tripartite motif containing 29 2 2
MIRT570242 CPNE5 copine 5 2 2
MIRT572327 HSPB6 heat shock protein family B (small) member 6 2 2
MIRT572373 ATOX1 antioxidant 1 copper chaperone 2 2
MIRT575024 Tecpr2 tectonin beta-propeller repeat containing 2 2 5
MIRT576146 Hmox1 heme oxygenase 1 2 2
MIRT612443 SMOC2 SPARC related modular calcium binding 2 2 2
MIRT615404 VDAC2 voltage dependent anion channel 2 2 2
MIRT629114 CYCS cytochrome c, somatic 2 2
MIRT631362 FOXI2 forkhead box I2 2 2
MIRT639322 THBD thrombomodulin 2 2
MIRT643797 ABCC12 ATP binding cassette subfamily C member 12 2 2
MIRT669687 ABLIM1 actin binding LIM protein 1 2 2
MIRT670595 LLGL1 LLGL1, scribble cell polarity complex component 2 4
MIRT691190 NIF3L1 NGG1 interacting factor 3 like 1 2 2
MIRT691688 FLOT2 flotillin 2 2 2
MIRT697127 OTUD5 OTU deubiquitinase 5 2 2
MIRT700814 PHLDA2 pleckstrin homology like domain family A member 2 2 2
MIRT701248 NUP35 nucleoporin 35 2 2
MIRT702362 KLHL15 kelch like family member 15 2 2
MIRT703325 GDPD5 glycerophosphodiester phosphodiesterase domain containing 5 2 2
MIRT706060 PKD1 polycystin 1, transient receptor potential channel interacting 2 2
MIRT710475 CDH5 cadherin 5 2 2
MIRT713018 SLC4A2 solute carrier family 4 member 2 2 2
MIRT716427 RAB15 RAB15, member RAS oncogene family 2 2
MIRT718093 ABHD12 abhydrolase domain containing 12 2 2
MIRT718542 PIGQ phosphatidylinositol glycan anchor biosynthesis class Q 2 2
MIRT719122 CACFD1 calcium channel flower domain containing 1 2 2
MIRT721393 LDLRAD4 low density lipoprotein receptor class A domain containing 4 2 2
MIRT736283 CDC42 cell division cycle 42 2 0
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-6132 Vemurafenib 42611257 NSC761431 approved resistant High Melanoma cell line (A375, IGR37, 501Mel)
hsa-miR-6132 Dabrafenib 44462760 NSC764134 approved resistant High Melanoma cell line (A375, IGR37, 501Mel)
hsa-miR-6132 Paclitaxel 36314 NSC125973 approved resistant High Non-Small Cell Lung Cancer cell line (A549)
hsa-miR-6132 Cisplatin 5460033 NSC119875 approved resistant High Hypopharyngeal Cancer cell line (FaDu)
hsa-miR-6132 Temozolomide 5394 NSC362856 approved sensitive cell line (U251)
hsa-miR-6132 Cisplatin 5460033 NSC119875 approved sensitive cell line
hsa-miR-6132 Doxorubicin 31703 NSC123127 approved resistant cell line (HS578T)
hsa-miR-6132 Osimertinib 71496458 NSC779217 approved resistant cell line (H1975)
hsa-miR-6132 Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (100 ng/ml)

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