pre-miRNA Information
pre-miRNA hsa-mir-2115   
Genomic Coordinates chr3: 48316360 - 48316459
Description Homo sapiens miR-2115 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-2115-5p
Sequence 21| AGCUUCCAUGACUCCUGAUGGA |42
Evidence Experimental
Experiments 454
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs372327506 9 dbSNP
rs1259362191 10 dbSNP
rs1215292065 12 dbSNP
rs1197671843 16 dbSNP
rs1468768022 20 dbSNP
rs1273755407 21 dbSNP
Putative Targets

miRNA Expression profile
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol MRPL45   
Synonyms L45mt, MRP-L45
Description mitochondrial ribosomal protein L45
Transcript NM_032351   
Expression
Putative miRNA Targets on MRPL45
3'UTR of MRPL45
(miRNA target sites are highlighted)
>MRPL45|NM_032351|3'UTR
   1 TGACAAAAATGACTTCTAGGGTGAAGCCTGGGTGATGAGGCTGCTGGAAGCTTTGAAGTCTCCCATTCCCCTCATGCTAT
  81 AAAAAGAACTACCTTTGTTCTCTCCCATCCTGCTCAGGTCTTTTCAGCAGTCTCATCATCAGCAACCATGACTGATGACT
 161 GGGCCCTAGCAGGTGGCAGGTATAACATGGCCATGGACACTCTTCTTTTTTAAATTTTATGTCTAGCTTCTGAGTCTAGA
 241 TGAAAGACAGTATGTTTCAGAGAACATTGGATATCAGTTTTTCCCACAGCAGGGACTGTGAGAGACAACCAGCAGCATCC
 321 TCTTTGTAATCACAGGGCAGGGATCAGAGTTTGAAATGAAATGTTGTCAGGGTGTTGGAAAAATTTTGGTGAGTTCTGCA
 401 CATTTCCCCTGGTTCAGGCTGGGCATGGACCAGCCTTCAGATGGCAGAAGTGGAAGATGAGCCTACTTGTGAGCGATGTG
 481 ACTTTAAGGAAATGAAGACTGGGGAAGAATAATTAGTGTTTATAAGACATTTAAGAGGCCCTTTTTCATATACTGACTCA
 561 CTGATGAATCAGCATTTGCATTTTATGGAAAAATATAAATCCAAAGAAATAATTTATCCCTTAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' aggUAGUCCUCAGUACCUUCGa 5'
             || |||  |  ||||||| 
Target 5' gtgATGAGG-CTGCTGGAAGCt 3'
32 - 52 150.00 -16.00
2
miRNA  3' agGUAGUCCUCAGU----ACCUUCga 5'
            | ||||  | ||    ||||||  
Target 5' gcCTTCAGATGGCAGAAGTGGAAGat 3'
433 - 458 134.00 -15.70
3
miRNA  3' agGUAGUCCUCAGUACCUUcga 5'
            ::||||| ||  |||||   
Target 5' gtTGTCAGG-GTGTTGGAAaaa 3'
363 - 383 123.00 -12.62
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs200734868 1 dbSNP
rs760585576 3 dbSNP
rs766154498 9 dbSNP
rs753669889 13 dbSNP
rs373033177 18 dbSNP
rs935752677 24 dbSNP
rs369593896 26 dbSNP
rs1165304817 27 dbSNP
rs752326050 29 dbSNP
rs539916887 35 dbSNP
rs559859866 38 dbSNP
rs780447318 43 dbSNP
rs1412989953 51 dbSNP
rs1238352098 61 dbSNP
rs750268773 68 dbSNP
rs1476124500 72 dbSNP
rs758183868 78 dbSNP
rs531969228 81 dbSNP
rs1192272133 89 dbSNP
rs1487137513 89 dbSNP
rs1270587928 94 dbSNP
rs940690768 117 dbSNP
rs796256520 122 dbSNP
rs1322638367 135 dbSNP
rs1289822391 138 dbSNP
rs766191288 142 dbSNP
rs1227066043 147 dbSNP
rs1320587836 150 dbSNP
rs551844624 157 dbSNP
rs891566495 159 dbSNP
rs1294991622 173 dbSNP
rs1338713483 181 dbSNP
rs1393828666 187 dbSNP
rs1295443354 188 dbSNP
rs1436956042 199 dbSNP
rs12094 203 dbSNP
rs10568 206 dbSNP
rs1349046424 213 dbSNP
rs1479275407 219 dbSNP
rs1321301552 221 dbSNP
rs907313968 231 dbSNP
rs938729713 240 dbSNP
rs1408489771 242 dbSNP
rs1055865001 250 dbSNP
rs933798964 253 dbSNP
rs186947847 265 dbSNP
rs191016452 268 dbSNP
rs1007250193 290 dbSNP
rs1246533895 292 dbSNP
rs1219798400 298 dbSNP
rs1459526903 321 dbSNP
rs1022295371 323 dbSNP
rs1323949917 325 dbSNP
rs1218102226 326 dbSNP
rs1332802690 337 dbSNP
rs1233875516 346 dbSNP
rs903432179 348 dbSNP
rs1220071376 352 dbSNP
rs1367142271 359 dbSNP
rs1016920191 363 dbSNP
rs999630140 367 dbSNP
rs1337224360 368 dbSNP
rs551270455 371 dbSNP
rs567843605 380 dbSNP
rs1030613644 384 dbSNP
rs994197020 390 dbSNP
rs1360351956 396 dbSNP
rs77253791 410 dbSNP
rs751502987 412 dbSNP
rs1422607842 413 dbSNP
rs1192229563 432 dbSNP
rs576960361 433 dbSNP
rs1211328952 434 dbSNP
rs953342985 442 dbSNP
rs14911 455 dbSNP
rs868240274 458 dbSNP
rs182353543 460 dbSNP
rs1023848166 473 dbSNP
rs866117436 475 dbSNP
rs571723908 476 dbSNP
rs372681721 478 dbSNP
rs972497547 479 dbSNP
rs1311898909 488 dbSNP
rs969659763 508 dbSNP
rs1200179527 510 dbSNP
rs1377301556 523 dbSNP
rs780712074 526 dbSNP
rs980121503 531 dbSNP
rs1372949888 542 dbSNP
rs1381229967 552 dbSNP
rs1302983451 556 dbSNP
rs1474633648 558 dbSNP
rs1173221995 569 dbSNP
rs567011745 577 dbSNP
rs140365000 604 dbSNP
rs760371005 607 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HeLa
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in Chi_ControlB_2A8_130_50. RNA binding protein: AGO. Condition:HeLa cell Control B ...

- Chi SW; Zang JB; Mele A; Darnell RB, 2009, Nature.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' aggUAGUCCUCAGUACCUUcga 5'
             || |||  |  |||||   
Target 5' gugAUGAGG-CUGCUGGAA--- 3'
3 - 20
Article - Chi SW; Zang JB; Mele A; Darnell RB
- Nature, 2009
MicroRNAs (miRNAs) have critical roles in the regulation of gene expression; however, as miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA, predicting target mRNAs is a major challenge. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein-RNA interaction sites. Here we use HITS-CLIP to covalently crosslink native argonaute (Ago, also called Eif2c) protein-RNA complexes in mouse brain. This produced two simultaneous data sets-Ago-miRNA and Ago-mRNA binding sites-that were combined with bioinformatic analysis to identify interaction sites between miRNA and target mRNA. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Ago HITS-CLIP provides a general platform for exploring the specificity and range of miRNA action in vivo, and identifies precise sequences for targeting clinically relevant miRNA-mRNA interactions.
LinkOut: [PMID: 19536157]
CLIP-seq Support 1 for dataset Chi_ControlB_2A8_130_50
Method / RBP HITS-CLIP / AGO
Cell line / Condition HeLa / HeLa cell Control B
Location of target site ENST00000312513.5 | 3UTR | GGGUGAUGAGGCUGCUGGAA
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 19536157 / Chi_HITSCLIP
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
BLCA 0.903 0.14 1.000 0.5 3 Click to see details
BRCA -0.314 0.16 -0.231 0.24 12 Click to see details
LUSC -0.097 0.41 -0.381 0.18 8 Click to see details
HNSC 0.101 0.42 0.143 0.39 6 Click to see details
HNSC 0.101 0.42 0.143 0.39 6 Click to see details
HNSC 0.101 0.42 0.143 0.39 6 Click to see details
HNSC 0.101 0.42 0.143 0.39 6 Click to see details
84 hsa-miR-2115-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT155281 IFNAR2 interferon alpha and beta receptor subunit 2 2 6
MIRT257301 MYLIP myosin regulatory light chain interacting protein 2 8
MIRT441907 SEPN1 selenoprotein N 2 2
MIRT442300 ZNF496 zinc finger protein 496 2 2
MIRT468235 SGK1 serum/glucocorticoid regulated kinase 1 2 2
MIRT469331 RGP1 RGP1 homolog, RAB6A GEF complex partner 1 2 2
MIRT470203 PSAT1 phosphoserine aminotransferase 1 2 6
MIRT475945 GXYLT1 glucoside xylosyltransferase 1 2 4
MIRT481449 ARRB2 arrestin beta 2 2 2
MIRT497440 SLC16A10 solute carrier family 16 member 10 2 2
MIRT498270 KIAA1644 KIAA1644 2 2
MIRT499225 VAV3 vav guanine nucleotide exchange factor 3 2 4
MIRT501742 NSD1 nuclear receptor binding SET domain protein 1 2 2
MIRT501762 NRF1 nuclear respiratory factor 1 2 6
MIRT527902 B3GALT5 beta-1,3-galactosyltransferase 5 2 4
MIRT528559 DNAAF3 dynein axonemal assembly factor 3 2 2
MIRT531252 PDF peptide deformylase, mitochondrial 2 2
MIRT539490 ACTN4 actinin alpha 4 2 2
MIRT550683 YARS tyrosyl-tRNA synthetase 2 2
MIRT573441 APOPT1 apoptogenic 1, mitochondrial 2 2
MIRT616740 DCTN5 dynactin subunit 5 2 2
MIRT617626 RAB3IP RAB3A interacting protein 2 2
MIRT620780 MT1A metallothionein 1A 2 2
MIRT623174 NAA50 N(alpha)-acetyltransferase 50, NatE catalytic subunit 2 2
MIRT624880 AASDHPPT aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase 2 2
MIRT625799 MDC1 mediator of DNA damage checkpoint 1 2 2
MIRT629790 CHRFAM7A CHRNA7 (exons 5-10) and FAM7A (exons A-E) fusion 2 2
MIRT630535 AGO3 argonaute 3, RISC catalytic component 2 2
MIRT630762 ZNF445 zinc finger protein 445 2 2
MIRT630921 UNC93A unc-93 homolog A 2 2
MIRT630949 PANK1 pantothenate kinase 1 2 2
MIRT631688 NQO2 N-ribosyldihydronicotinamide:quinone reductase 2 2 2
MIRT633898 FGF10 fibroblast growth factor 10 2 2
MIRT635935 PLA2G12A phospholipase A2 group XIIA 2 2
MIRT636177 THBD thrombomodulin 2 2
MIRT636730 AFAP1 actin filament associated protein 1 2 2
MIRT638038 SHPK sedoheptulokinase 2 2
MIRT639164 LAMTOR3 late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 2 2
MIRT639260 MANEAL mannosidase endo-alpha like 2 2
MIRT639327 C9orf41 carnosine N-methyltransferase 1 2 2
MIRT639849 YY1 YY1 transcription factor 2 2
MIRT641474 B4GALNT3 beta-1,4-N-acetyl-galactosaminyltransferase 3 2 2
MIRT642783 CHCHD3 coiled-coil-helix-coiled-coil-helix domain containing 3 2 2
MIRT643653 MYOCD myocardin 2 2
MIRT645717 PTPRF protein tyrosine phosphatase, receptor type F 2 2
MIRT648018 SLCO4C1 solute carrier organic anion transporter family member 4C1 2 2
MIRT648236 C15orf40 chromosome 15 open reading frame 40 2 2
MIRT648563 MEMO1 mediator of cell motility 1 2 2
MIRT648731 HIST1H2BD histone cluster 1 H2B family member d 2 2
MIRT650179 LILRA2 leukocyte immunoglobulin like receptor A2 2 2
MIRT650721 TNFSF8 TNF superfamily member 8 2 2
MIRT652179 TRIM44 tripartite motif containing 44 2 2
MIRT654272 RFFL ring finger and FYVE like domain containing E3 ubiquitin protein ligase 2 2
MIRT654493 RAD51L3-RFFL RAD51L3-RFFL readthrough 2 2
MIRT654861 PPM1F protein phosphatase, Mg2+/Mn2+ dependent 1F 2 2
MIRT656392 MCU mitochondrial calcium uniporter 2 2
MIRT656623 LRRC15 leucine rich repeat containing 15 2 2
MIRT657085 JMY junction mediating and regulatory protein, p53 cofactor 2 2
MIRT659793 CBLB Cbl proto-oncogene B 2 2
MIRT660155 BRCC3 BRCA1/BRCA2-containing complex subunit 3 2 2
MIRT662444 SERPINB5 serpin family B member 5 2 2
MIRT663116 SPTA1 spectrin alpha, erythrocytic 1 2 2
MIRT665759 TMEM43 transmembrane protein 43 2 2
MIRT666357 SIKE1 suppressor of IKBKE 1 2 2
MIRT668521 ESRRG estrogen related receptor gamma 2 2
MIRT677776 FKTN fukutin 2 2
MIRT678900 TTLL12 tubulin tyrosine ligase like 12 2 2
MIRT702825 HOOK3 hook microtubule tethering protein 3 2 2
MIRT704424 CTNNBIP1 catenin beta interacting protein 1 2 2
MIRT709470 KRTAP19-1 keratin associated protein 19-1 2 2
MIRT710939 MRPL45 mitochondrial ribosomal protein L45 2 2
MIRT713306 TYRP1 tyrosinase related protein 1 2 2
MIRT716932 FAM13A family with sequence similarity 13 member A 2 2
MIRT717539 PYGO2 pygopus family PHD finger 2 2 2
MIRT718060 CYP3A5 cytochrome P450 family 3 subfamily A member 5 2 2
MIRT718934 TRIM66 tripartite motif containing 66 2 2
MIRT719770 ZNF236 zinc finger protein 236 2 2
MIRT720164 PNPO pyridoxamine 5'-phosphate oxidase 2 2
MIRT721280 RAD54L2 RAD54 like 2 2 2
MIRT721359 ENTHD1 ENTH domain containing 1 2 2
MIRT721506 CARHSP1 calcium regulated heat stable protein 1 2 2
MIRT721920 LINGO2 leucine rich repeat and Ig domain containing 2 2 2
MIRT722791 FUT4 fucosyltransferase 4 2 2
MIRT723933 SVOP SV2 related protein 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-2115 Docetaxel+Cisplatin+5-Fluorouracil sensitive tissue (hypopharyngeal squamous cell carcinoma)
hsa-mir-2115 Decitabine 451668 approved sensitive tissue (esopheageal cancer)
hsa-miR-2115-5p Paclitaxel 36314 NSC125973 approved resistant cell line (BAS)
hsa-miR-2115-5p Tamoxifen 2733525 NSC180973 approved sensitive cell line (LCC2)
hsa-miR-2115-5p Cisplatin 5460033 NSC119875 approved sensitive cell line (A549)
hsa-miR-2115-5p Ceritinib 57379345 NSC776422 approved resistant cell line (H3122)

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