pre-miRNA Information
pre-miRNA hsa-mir-6511b-1   
Genomic Coordinates chr16: 2106669 - 2106753
Description Homo sapiens miR-6511b-1 stem-loop
Comment None
RNA Secondary Structure
pre-miRNA hsa-mir-6511b-2   
Genomic Coordinates chr16: 15134075 - 15134145
Description Homo sapiens miR-6511b-2 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-6511b-3p
Sequence 53| CCUCACCACCCCUUCUGCCUGCA |75
Evidence Experimental
Experiments Illumina
DRVs in miRNA
Mutant ID Mutant Position Mutant Source
993639 5 ClinVar
1196654 15 ClinVar
256998 15 ClinVar
433980 16 ClinVar
586295 18 ClinVar
433979 19 ClinVar
COSM3948368 17 COSMIC
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs769351879 1 dbSNP
rs1001283562 2 dbSNP
rs763611191 3 dbSNP
rs1465530682 4 dbSNP
rs1227492873 4 dbSNP
rs745502224 5 dbSNP
rs551972801 6 dbSNP
rs538162642 6 dbSNP
rs751438298 7 dbSNP
rs1285683081 7 dbSNP
rs1340898267 8 dbSNP
rs4018164 9 dbSNP
rs370568831 9 dbSNP
rs1180919709 10 dbSNP
rs1339482497 10 dbSNP
rs758216756 11 dbSNP
rs1323026290 11 dbSNP
rs1239445091 12 dbSNP
rs1310601001 12 dbSNP
rs1431093969 13 dbSNP
rs752690118 15 dbSNP
rs1371790714 15 dbSNP
rs746463189 16 dbSNP
rs1209415208 17 dbSNP
rs761022804 17 dbSNP
rs1242038386 17 dbSNP
rs370411414 18 dbSNP
rs759723934 19 dbSNP
rs1428318191 19 dbSNP
rs1416441031 20 dbSNP
rs754040914 21 dbSNP
rs776062795 21 dbSNP
rs1273686335 22 dbSNP
rs1182772456 22 dbSNP
rs1472619959 23 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol MED7   
Synonyms ARC34, CRSP33, CRSP9
Description mediator complex subunit 7
Transcript NM_001100816   
Other Transcripts NM_004270   
Expression
Putative miRNA Targets on MED7
3'UTR of MED7
(miRNA target sites are highlighted)
>MED7|NM_001100816|3'UTR
   1 AAGATGTTTCTTTTTCTTTTTTTCCTTTTGATAATAGCATCATATATTAGTTCATTTTCTTTTGGACAGTCTTAAGAGAA
  81 GTTTCACTAAAAATGTAAACAGCTTTAATCTTGACTCCAAATTTTTCAATTATGAGATGTCATAGGCAGTAATTTCGCTG
 161 TATAACAAGCATAGACAAATGAGTGTCCCTGCACTAAGAAGAATCACTTTAAAAAGCAAAGTGTTAGCTGCTGTTGTATG
 241 GGACATTCCTATGTTTTAGAGTTGCAGTAAAACTTTGATGATAACCTCAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN26984150 16 COSMIC
COSN30460609 16 COSMIC
COSN31526153 16 COSMIC
COSN26984151 17 COSMIC
COSN30182798 35 COSMIC
COSN31495528 36 COSMIC
COSN30189392 37 COSMIC
COSN30154928 46 COSMIC
COSN30522886 89 COSMIC
COSN30511900 116 COSMIC
COSN22208279 156 COSMIC
COSN18806911 180 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs746321069 3 dbSNP
rs1232442914 4 dbSNP
rs1222386068 6 dbSNP
rs774853668 9 dbSNP
rs1453346557 10 dbSNP
rs1275472565 11 dbSNP
rs1173435028 14 dbSNP
rs759613123 14 dbSNP
rs770361852 17 dbSNP
rs748793891 23 dbSNP
rs1043892 24 dbSNP
rs1043894 25 dbSNP
rs1354172244 29 dbSNP
rs553002745 34 dbSNP
rs1373076585 35 dbSNP
rs1008006735 37 dbSNP
rs1412262575 45 dbSNP
rs1344768476 46 dbSNP
rs1435907389 53 dbSNP
rs879886222 54 dbSNP
rs894007902 59 dbSNP
rs1232053098 66 dbSNP
rs1274147159 69 dbSNP
rs1345534685 71 dbSNP
rs1263679496 75 dbSNP
rs1243961810 82 dbSNP
rs1053865228 86 dbSNP
rs937781922 90 dbSNP
rs1208846832 92 dbSNP
rs1249297162 94 dbSNP
rs1294869047 95 dbSNP
rs1407724598 108 dbSNP
rs890904092 110 dbSNP
rs904924678 113 dbSNP
rs1198856887 117 dbSNP
rs1041428924 118 dbSNP
rs1378701437 127 dbSNP
rs9007 129 dbSNP
rs574191904 132 dbSNP
rs771151158 137 dbSNP
rs908198262 144 dbSNP
rs557515581 148 dbSNP
rs537370276 156 dbSNP
rs1322781609 157 dbSNP
rs369252857 158 dbSNP
rs1388666955 162 dbSNP
rs747432582 171 dbSNP
rs1331554029 174 dbSNP
rs185611895 180 dbSNP
rs1235150813 182 dbSNP
rs879261479 187 dbSNP
rs919780803 191 dbSNP
rs975219950 192 dbSNP
rs1269598635 197 dbSNP
rs1332101723 202 dbSNP
rs1208663668 206 dbSNP
rs1268670236 206 dbSNP
rs557800004 207 dbSNP
rs1453791159 208 dbSNP
rs949514107 213 dbSNP
rs1251608625 217 dbSNP
rs866001715 221 dbSNP
rs534556910 226 dbSNP
rs1458835086 236 dbSNP
rs1161735404 241 dbSNP
rs964799073 248 dbSNP
rs1457482478 249 dbSNP
rs565781190 251 dbSNP
rs1391405627 253 dbSNP
rs987570737 259 dbSNP
rs1347654221 273 dbSNP
rs767779387 273 dbSNP
rs1410791267 274 dbSNP
rs1182067212 276 dbSNP
rs982877545 280 dbSNP
rs1439115632 283 dbSNP
rs1264616473 286 dbSNP
rs768405631 288 dbSNP
rs549144057 290 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HeLa
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in Chi_124B_2A8_130_50. RNA binding protein: AGO. Condition:HeLa cell miR-124 + B HITS-CLIP data was present in Chi_ControlB_2A8_130_50. RNA binding protein: AGO. Condition:HeLa cell Control B ...

- Chi SW; Zang JB; Mele A; Darnell RB, 2009, Nature.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' acguccgucuuccccACCACUCc 5'
                         ||||||| 
Target 5' -------------uaUGGUGAGg 3'
1 - 10
Article - Chi SW; Zang JB; Mele A; Darnell RB
- Nature, 2009
MicroRNAs (miRNAs) have critical roles in the regulation of gene expression; however, as miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA, predicting target mRNAs is a major challenge. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein-RNA interaction sites. Here we use HITS-CLIP to covalently crosslink native argonaute (Ago, also called Eif2c) protein-RNA complexes in mouse brain. This produced two simultaneous data sets-Ago-miRNA and Ago-mRNA binding sites-that were combined with bioinformatic analysis to identify interaction sites between miRNA and target mRNA. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Ago HITS-CLIP provides a general platform for exploring the specificity and range of miRNA action in vivo, and identifies precise sequences for targeting clinically relevant miRNA-mRNA interactions.
LinkOut: [PMID: 19536157]
CLIP-seq Support 1 for dataset Chi_124B_2A8_130_50
Method / RBP HITS-CLIP / AGO
Cell line / Condition HeLa / HeLa cell miR-124 + B
Location of target site ENST00000286317.5 | 3UTR | UAUGGUGAGGGAGGAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 19536157 / Chi_HITSCLIP
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset Chi_ControlB_2A8_130_50
Method / RBP HITS-CLIP / AGO
Cell line / Condition HeLa / HeLa cell Control B
Location of target site ENST00000286317.5 | 3UTR | UAUGGUGAGGGAGGAGGUU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 19536157 / Chi_HITSCLIP
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
69 hsa-miR-6511b-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT059269 CELF1 CUGBP Elav-like family member 1 2 2
MIRT061287 IPO7 importin 7 2 2
MIRT115533 MAZ MYC associated zinc finger protein 2 2
MIRT345986 BIRC5 baculoviral IAP repeat containing 5 2 8
MIRT379536 HNRNPK heterogeneous nuclear ribonucleoprotein K 2 2
MIRT442491 RBBP5 RB binding protein 5, histone lysine methyltransferase complex subunit 2 8
MIRT443701 HUNK hormonally up-regulated Neu-associated kinase 2 4
MIRT459167 HSPA6 heat shock protein family A (Hsp70) member 6 2 21
MIRT497179 ZBTB40 zinc finger and BTB domain containing 40 2 2
MIRT497846 GATA6 GATA binding protein 6 2 4
MIRT519625 ZNF781 zinc finger protein 781 2 2
MIRT519838 ZFP69B ZFP69 zinc finger protein B 2 4
MIRT528560 DNAAF3 dynein axonemal assembly factor 3 2 2
MIRT530718 ORMDL3 ORMDL sphingolipid biosynthesis regulator 3 2 2
MIRT530810 GPR182 G protein-coupled receptor 182 2 2
MIRT533265 VAV3 vav guanine nucleotide exchange factor 3 2 4
MIRT533726 TMEM246 transmembrane protein 246 2 2
MIRT535547 P2RY2 purinergic receptor P2Y2 2 2
MIRT536019 MCUR1 mitochondrial calcium uniporter regulator 1 2 2
MIRT539494 ACTN4 actinin alpha 4 2 2
MIRT541793 MGAT5 mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase 2 8
MIRT554509 RUNX1T1 RUNX1 translocation partner 1 2 2
MIRT558784 CEP55 centrosomal protein 55 2 2
MIRT560013 ZNF525 zinc finger protein 525 2 2
MIRT560078 ZNF195 zinc finger protein 195 2 2
MIRT570135 IL1RL2 interleukin 1 receptor like 2 2 2
MIRT570890 ZNF780A zinc finger protein 780A 2 2
MIRT607972 SNX22 sorting nexin 22 2 2
MIRT608104 CRISPLD2 cysteine rich secretory protein LCCL domain containing 2 2 2
MIRT610471 ADAMTS13 ADAM metallopeptidase with thrombospondin type 1 motif 13 2 4
MIRT611134 GGT7 gamma-glutamyltransferase 7 2 2
MIRT611448 NRIP3 nuclear receptor interacting protein 3 2 2
MIRT613019 GABPB1 GA binding protein transcription factor beta subunit 1 2 4
MIRT615753 C6 complement C6 2 2
MIRT620464 CERS6 ceramide synthase 6 2 2
MIRT632248 VPS41 VPS41, HOPS complex subunit 2 2
MIRT636099 ZDHHC22 zinc finger DHHC-type containing 22 2 2
MIRT637452 ZNF324B zinc finger protein 324B 2 2
MIRT638927 CALCOCO2 calcium binding and coiled-coil domain 2 2 2
MIRT646768 WDR3 WD repeat domain 3 2 2
MIRT652610 TIMM8A translocase of inner mitochondrial membrane 8A 2 2
MIRT652868 TAB1 TGF-beta activated kinase 1 (MAP3K7) binding protein 1 2 2
MIRT653655 SLC27A4 solute carrier family 27 member 4 2 2
MIRT657089 JMY junction mediating and regulatory protein, p53 cofactor 2 2
MIRT657884 GFPT1 glutamine--fructose-6-phosphate transaminase 1 2 2
MIRT662919 MED18 mediator complex subunit 18 2 2
MIRT685622 C12orf49 chromosome 12 open reading frame 49 2 2
MIRT687427 NRIP1 nuclear receptor interacting protein 1 2 2
MIRT692304 CNNM3 cyclin and CBS domain divalent metal cation transport mediator 3 2 2
MIRT695127 PRY2 PTPN13-like, Y-linked 2 2 2
MIRT695144 PRY PTPN13-like, Y-linked 2 2
MIRT696286 IER3IP1 immediate early response 3 interacting protein 1 2 2
MIRT699350 SLC35E1 solute carrier family 35 member E1 2 2
MIRT709901 AGO1 argonaute 1, RISC catalytic component 2 2
MIRT710877 SLC25A42 solute carrier family 25 member 42 2 2
MIRT711365 MED7 mediator complex subunit 7 2 2
MIRT711444 FRMPD3 FERM and PDZ domain containing 3 2 2
MIRT713221 RCAN2 regulator of calcineurin 2 2 2
MIRT713281 LAIR1 leukocyte associated immunoglobulin like receptor 1 2 2
MIRT714195 TRAF7 TNF receptor associated factor 7 2 2
MIRT715152 IL12B interleukin 12B 2 2
MIRT719197 CASP10 caspase 10 2 2
MIRT719469 SRF serum response factor 2 2
MIRT720197 MPP6 membrane palmitoylated protein 6 2 2
MIRT720449 SLC16A5 solute carrier family 16 member 5 2 2
MIRT720461 RAB31 RAB31, member RAS oncogene family 2 2
MIRT721646 ZNF207 zinc finger protein 207 2 2
MIRT722001 CLLU1OS chronic lymphocytic leukemia up-regulated 1 opposite strand 2 2
MIRT725521 FAM229B family with sequence similarity 229 member B 2 2
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-miR-6511b-3p Imatinib 5291 NSC743414 approved sensitive High Chronic Myelogenous Leukemia tissue
hsa-miR-6511b-3p Gefitinib 123631 NSC715055 approved sensitive cell line (HCC827)
hsa-miR-6511b-3p Osimertinib 71496458 NSC779217 approved sensitive cell line (HCC827)
hsa-miR-6511b-3p Osimertinib 71496458 NSC779217 approved resistant cell line (PC9)
hsa-miR-6511b-3p Cisplatin 5460033 NSC119875 approved sensitive cell line (A2780)
hsa-miR-6511b-3p Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (1500 ng/ml)

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