pre-miRNA Information
pre-miRNA hsa-mir-4457   
Genomic Coordinates chr5: 1309310 - 1309377
Description Homo sapiens miR-4457 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4457
Sequence 43| UCACAAGGUAUUGACUGGCGUA |64
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1031676297 8 dbSNP
rs1249861366 14 dbSNP
rs115769169 17 dbSNP
rs968846507 19 dbSNP
rs540870271 20 dbSNP
rs993130937 22 dbSNP
Putative Targets

Gene Information
Gene Symbol TMEM81   
Synonyms HC3107, KVLA2788, UNQ2788
Description transmembrane protein 81
Transcript NM_203376   
Expression
Putative miRNA Targets on TMEM81
3'UTR of TMEM81
(miRNA target sites are highlighted)
>TMEM81|NM_203376|3'UTR
   1 CAGCTTCAAGAACTTAACAGCCTTGCTCCTGAAGAACTGGCTGCCCAGGAAGCCAAGCTAGCTTTTTAGGGGAGTGTTCC
  81 AGCTGCTGGTAGTGGATCAGCTTAGAGGGAACACTCCCACAGCCAAAAGAATGAGTGGGAGAAATGGAGGGGACAATCTC
 161 CTGGGAGCTATGCGCAGTAACCTAACTTCCTTATGTCCCATGGATCTCTTCCTGATCTTCCCTGCCCATTGGGTACCCAG
 241 GAAACTGCAAGCATTGCCTGTGTTCCTGGGAAGAGTTCTAAGAAGCTTGCATTCATTTTCTACCCTTTATGACTTGGATG
 321 CCTCCCCACCTCCATTTCCCCTCTTCTGAGCTGTGTATTCATGTAGAGGGATGTATTCAGCCTTTTTAGTGAACATTTTT
 401 TTTCAATAAAAGTAATTCACAGTAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' auGCG-GUCA----GUU-AUGGAACACu 5'
            :|| ||||    :|| | |||| || 
Target 5' taTGCGCAGTAACCTAACTTCCTTATGt 3'
169 - 196 122.00 -11.06
2
miRNA  3' augcggUCAGUUAU-GGAACacu 5'
                | |:|| | |||||   
Target 5' tcaagaACTTAACAGCCTTGctc 3'
6 - 28 112.00 -5.62
3
miRNA  3' auGCGGUCAGUUAUGGA-ACAcu 5'
            :|| || || |:||| |||  
Target 5' acTGCAAG-CATTGCCTGTGTtc 3'
244 - 265 111.00 -11.60
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN511200 1 COSMIC
COSN30139601 14 COSMIC
COSN30614841 14 COSMIC
COSN30469337 28 COSMIC
COSN26999131 43 COSMIC
COSN30462616 44 COSMIC
COSN30160387 45 COSMIC
COSN26999130 63 COSMIC
COSN31525460 71 COSMIC
COSN7983674 89 COSMIC
COSN30135303 129 COSMIC
COSN31599647 146 COSMIC
COSN30527518 166 COSMIC
COSN32065138 238 COSMIC
COSN7199137 347 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs199727080 10 dbSNP
rs1433013101 20 dbSNP
rs151238335 21 dbSNP
rs1362663405 26 dbSNP
rs773391999 28 dbSNP
rs772089533 29 dbSNP
rs1329505795 35 dbSNP
rs762933245 39 dbSNP
rs1173897147 46 dbSNP
rs940279710 52 dbSNP
rs1467109013 54 dbSNP
rs1291782969 56 dbSNP
rs887346624 72 dbSNP
rs1036000623 80 dbSNP
rs1370070023 81 dbSNP
rs772678796 83 dbSNP
rs906189587 97 dbSNP
rs1326570314 101 dbSNP
rs931433577 110 dbSNP
rs199577492 115 dbSNP
rs185457648 126 dbSNP
rs1386607196 130 dbSNP
rs1217635811 143 dbSNP
rs947956938 147 dbSNP
rs372994426 151 dbSNP
rs991504131 152 dbSNP
rs938700681 159 dbSNP
rs1401463978 162 dbSNP
rs1264255045 165 dbSNP
rs1488454735 169 dbSNP
rs539551661 170 dbSNP
rs557171706 173 dbSNP
rs989677492 174 dbSNP
rs1193288968 183 dbSNP
rs1424422297 189 dbSNP
rs1254173330 193 dbSNP
rs1162571477 194 dbSNP
rs1349179085 199 dbSNP
rs1458373798 200 dbSNP
rs538069347 208 dbSNP
rs977378554 211 dbSNP
rs966684632 216 dbSNP
rs192900771 220 dbSNP
rs1398991122 222 dbSNP
rs1021290173 223 dbSNP
rs1278513532 225 dbSNP
rs772588576 228 dbSNP
rs748595389 229 dbSNP
rs1245716406 234 dbSNP
rs924720586 252 dbSNP
rs1226073254 260 dbSNP
rs142284266 261 dbSNP
rs1214479642 263 dbSNP
rs958078643 267 dbSNP
rs977487849 268 dbSNP
rs1294386843 281 dbSNP
rs1487551577 286 dbSNP
rs535379914 288 dbSNP
rs1216124652 290 dbSNP
rs536113807 292 dbSNP
rs1019357403 300 dbSNP
rs996574884 304 dbSNP
rs900905203 314 dbSNP
rs1424263394 324 dbSNP
rs1346952184 329 dbSNP
rs1304038114 331 dbSNP
rs546199250 346 dbSNP
rs1378243055 347 dbSNP
rs1418926211 351 dbSNP
rs1298609367 353 dbSNP
rs1362023294 365 dbSNP
rs1362981918 372 dbSNP
rs1019320479 375 dbSNP
rs1379339705 387 dbSNP
rs12028064 389 dbSNP
rs1317826805 395 dbSNP
rs45462698 396 dbSNP
rs1431576526 397 dbSNP
rs1347124608 401 dbSNP
rs1027841493 404 dbSNP
rs1448888042 405 dbSNP
rs757819514 405 dbSNP
rs1208294817 408 dbSNP
rs1236961002 414 dbSNP
rs1483655865 418 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HeLa
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in Chi_ControlA_2A8_130_50. RNA binding protein: AGO. Condition:HeLa cell Control A ...

- Chi SW; Zang JB; Mele A; Darnell RB, 2009, Nature.

Article - Chi SW; Zang JB; Mele A; Darnell RB
- Nature, 2009
MicroRNAs (miRNAs) have critical roles in the regulation of gene expression; however, as miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA, predicting target mRNAs is a major challenge. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein-RNA interaction sites. Here we use HITS-CLIP to covalently crosslink native argonaute (Ago, also called Eif2c) protein-RNA complexes in mouse brain. This produced two simultaneous data sets-Ago-miRNA and Ago-mRNA binding sites-that were combined with bioinformatic analysis to identify interaction sites between miRNA and target mRNA. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Ago HITS-CLIP provides a general platform for exploring the specificity and range of miRNA action in vivo, and identifies precise sequences for targeting clinically relevant miRNA-mRNA interactions.
LinkOut: [PMID: 19536157]
CLIP-seq Support 1 for dataset Chi_ControlA_2A8_130_50
Method / RBP HITS-CLIP / AGO
Cell line / Condition HeLa / HeLa cell Control A
Location of target site ENST00000367167.3 | 3UTR | UUGUGAAAAAUAAAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 19536157 / Chi_HITSCLIP
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
83 hsa-miR-4457 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT098032 SOBP sine oculis binding protein homolog 2 2
MIRT202580 PCMTD2 protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 2 2 6
MIRT247137 WEE1 WEE1 G2 checkpoint kinase 2 4
MIRT349266 PTBP1 polypyrimidine tract binding protein 1 2 2
MIRT363756 EIF4EBP1 eukaryotic translation initiation factor 4E binding protein 1 2 2
MIRT443586 FAM84B family with sequence similarity 84 member B 2 2
MIRT452333 EIF5AL1 eukaryotic translation initiation factor 5A-like 1 2 2
MIRT453864 ZBTB40 zinc finger and BTB domain containing 40 2 4
MIRT471486 PDE4D phosphodiesterase 4D 2 4
MIRT476290 GMFB glia maturation factor beta 2 8
MIRT479897 CCDC117 coiled-coil domain containing 117 2 4
MIRT484251 ANK1 ankyrin 1 2 2
MIRT491473 TMEM214 transmembrane protein 214 2 2
MIRT493804 GAN gigaxonin 2 6
MIRT499252 VAV3 vav guanine nucleotide exchange factor 3 2 4
MIRT502271 HNRNPA1 heterogeneous nuclear ribonucleoprotein A1 2 4
MIRT504651 RPL9 ribosomal protein L9 2 6
MIRT505211 UBN2 ubinuclein 2 2 8
MIRT508952 SNRPB small nuclear ribonucleoprotein polypeptides B and B1 2 4
MIRT512691 POP1 POP1 homolog, ribonuclease P/MRP subunit 2 2
MIRT513320 SCUBE3 signal peptide, CUB domain and EGF like domain containing 3 2 2
MIRT513870 HOXA5 homeobox A5 2 2
MIRT517342 ZNF529 zinc finger protein 529 2 4
MIRT518947 LSG1 large 60S subunit nuclear export GTPase 1 2 2
MIRT520867 SUGT1 SGT1 homolog, MIS12 kinetochore complex assembly cochaperone 2 2
MIRT528325 GIGYF2 GRB10 interacting GYF protein 2 2 2
MIRT531990 SLCO1B3 solute carrier organic anion transporter family member 1B3 2 2
MIRT533298 USP46 ubiquitin specific peptidase 46 2 2
MIRT545257 TRIM36 tripartite motif containing 36 2 4
MIRT547038 POGZ pogo transposable element derived with ZNF domain 2 2
MIRT556103 MOAP1 modulator of apoptosis 1 2 2
MIRT558321 DR1 down-regulator of transcription 1 2 2
MIRT558521 CSRNP3 cysteine and serine rich nuclear protein 3 2 2
MIRT560906 TMED10 transmembrane p24 trafficking protein 10 2 2
MIRT568448 ARPP19 cAMP regulated phosphoprotein 19 2 2
MIRT570586 OTUD7B OTU deubiquitinase 7B 2 2
MIRT572799 SIGLEC14 sialic acid binding Ig like lectin 14 2 2
MIRT573863 C9orf78 chromosome 9 open reading frame 78 2 2
MIRT575058 P2ry1 purinergic receptor P2Y, G-protein coupled 1 2 5
MIRT609931 SLC38A1 solute carrier family 38 member 1 2 4
MIRT610836 ZNF585A zinc finger protein 585A 2 4
MIRT611474 P2RY1 purinergic receptor P2Y1 2 7
MIRT613569 YY2 YY2 transcription factor 2 2
MIRT618626 GREB1 growth regulation by estrogen in breast cancer 1 2 2
MIRT620606 SAP30 Sin3A associated protein 30 2 2
MIRT621017 CLSTN3 calsyntenin 3 2 4
MIRT635314 FAM179A TOG array regulator of axonemal microtubules 2 2 2
MIRT635919 GLTSCR2 NOP53 ribosome biogenesis factor 2 2
MIRT640598 TM9SF4 transmembrane 9 superfamily member 4 2 2
MIRT641784 YWHAB tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta 2 4
MIRT644067 IQCE IQ motif containing E 2 2
MIRT648288 TRAPPC2L trafficking protein particle complex 2 like 2 2
MIRT658085 FOXR2 forkhead box R2 2 2
MIRT659077 DEPTOR DEP domain containing MTOR interacting protein 2 2
MIRT665307 ZBTB37 zinc finger and BTB domain containing 37 2 2
MIRT665975 SYTL4 synaptotagmin like 4 2 2
MIRT666302 SLC25A25 solute carrier family 25 member 25 2 2
MIRT674906 RASSF9 Ras association domain family member 9 2 2
MIRT680086 THAP1 THAP domain containing 1 2 2
MIRT681488 DIP2A disco interacting protein 2 homolog A 2 2
MIRT691244 DFNB59 pejvakin 2 2
MIRT692362 AGTRAP angiotensin II receptor associated protein 2 2
MIRT693035 MB21D1 Mab-21 domain containing 1 2 2
MIRT693837 STAT5A signal transducer and activator of transcription 5A 2 2
MIRT694479 LRTOMT leucine rich transmembrane and O-methyltransferase domain containing 2 2
MIRT696070 ZNF264 zinc finger protein 264 2 2
MIRT696578 TTC21B tetratricopeptide repeat domain 21B 2 2
MIRT696760 MTFMT mitochondrial methionyl-tRNA formyltransferase 2 2
MIRT697307 ZNF652 zinc finger protein 652 2 2
MIRT700152 RNF115 ring finger protein 115 2 2
MIRT701056 PARP2 poly(ADP-ribose) polymerase 2 2 2
MIRT701198 OTUD3 OTU deubiquitinase 3 2 2
MIRT701335 NSD1 nuclear receptor binding SET domain protein 1 2 2
MIRT702656 ITGA3 integrin subunit alpha 3 2 2
MIRT703618 FBXO45 F-box protein 45 2 2
MIRT704673 CHTOP chromatin target of PRMT1 2 2
MIRT708894 ZNF780A zinc finger protein 780A 2 2
MIRT711620 DGKH diacylglycerol kinase eta 2 2
MIRT713745 TMEM81 transmembrane protein 81 2 2
MIRT719712 CD101 CD101 molecule 2 2
MIRT720294 DLGAP3 DLG associated protein 3 2 2
MIRT722606 CCDC152 coiled-coil domain containing 152 2 2
MIRT724566 ACSBG1 acyl-CoA synthetase bubblegum family member 1 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-4 Dexamethasone approved 5743 Microarray primary rat thymocytes 20847043 2010 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-4457 Dabrafenib 44462760 NSC764134 approved sensitive cell line (A375)
hsa-miR-4457 Gefitinib 123631 NSC715055 approved sensitive cell line (PC9)
hsa-miR-4457 Osimertinib 71496458 NSC779217 approved sensitive cell line (PC9)
hsa-miR-4457 Osimertinib 71496458 NSC779217 approved sensitive cell line (H1975)

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