pre-miRNA Information
pre-miRNA hsa-mir-8067   
Genomic Coordinates chr15: 62304658 - 62304734
Description Homo sapiens miR-8067 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-8067
Sequence 11| CCUAGAAACUGUAAACUUAGUC |32
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1439702395 3 dbSNP
rs973468045 19 dbSNP
rs1474855764 20 dbSNP
rs183845458 20 dbSNP
Putative Targets

Gene Information
Gene Symbol RPL24   
Synonyms HEL-S-310, L24
Description ribosomal protein L24
Transcript NM_000986   
Expression
Putative miRNA Targets on RPL24
3'UTR of RPL24
(miRNA target sites are highlighted)
>RPL24|NM_000986|3'UTR
   1 ACTGGCAGATTAGATTTTTAAATAAAGATTGGATTATAACTCTAGAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' cugauucaaaugucaaAGAUCc 5'
                          ||||| 
Target 5' aagattggattataacTCTAGa 3'
25 - 46 100.00 -7.10
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN31524591 8 COSMIC
COSN31486243 42 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1489417286 10 dbSNP
rs748024194 13 dbSNP
rs774417834 23 dbSNP
rs1311421254 30 dbSNP
rs1299019781 31 dbSNP
rs768689678 36 dbSNP
rs1345576732 37 dbSNP
rs187855124 44 dbSNP
rs780211272 44 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HeLa
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in Chi_124B_2A8_130_50. RNA binding protein: AGO. Condition:HeLa cell miR-124 + B ...

- Chi SW; Zang JB; Mele A; Darnell RB, 2009, Nature.

Article - Chi SW; Zang JB; Mele A; Darnell RB
- Nature, 2009
MicroRNAs (miRNAs) have critical roles in the regulation of gene expression; however, as miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA, predicting target mRNAs is a major challenge. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein-RNA interaction sites. Here we use HITS-CLIP to covalently crosslink native argonaute (Ago, also called Eif2c) protein-RNA complexes in mouse brain. This produced two simultaneous data sets-Ago-miRNA and Ago-mRNA binding sites-that were combined with bioinformatic analysis to identify interaction sites between miRNA and target mRNA. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Ago HITS-CLIP provides a general platform for exploring the specificity and range of miRNA action in vivo, and identifies precise sequences for targeting clinically relevant miRNA-mRNA interactions.
LinkOut: [PMID: 19536157]
CLIP-seq Support 1 for dataset Chi_124B_2A8_130_50
Method / RBP HITS-CLIP / AGO
Cell line / Condition HeLa / HeLa cell miR-124 + B
Location of target site ENST00000495401.1 | 3UTR | GUGAUAGAAUUUUUUUUUUU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 19536157 / Chi_HITSCLIP
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
68 hsa-miR-8067 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT084581 BCL2L11 BCL2 like 11 2 4
MIRT088215 NUP50 nucleoporin 50 2 2
MIRT092085 ABHD5 abhydrolase domain containing 5 2 2
MIRT135269 TMBIM6 transmembrane BAX inhibitor motif containing 6 2 8
MIRT175259 PSAT1 phosphoserine aminotransferase 1 2 6
MIRT182508 KLHL20 kelch like family member 20 2 2
MIRT286224 TMEM97 transmembrane protein 97 2 4
MIRT301718 TEF TEF, PAR bZIP transcription factor 2 2
MIRT329381 CCNG1 cyclin G1 2 2
MIRT362146 MTRNR2L6 MT-RNR2-like 6 2 4
MIRT366561 EIF2S3 eukaryotic translation initiation factor 2 subunit gamma 2 2
MIRT442002 NDUFV3 NADH:ubiquinone oxidoreductase subunit V3 2 2
MIRT442039 TRPV2 transient receptor potential cation channel subfamily V member 2 2 2
MIRT443045 HPS4 HPS4, biogenesis of lysosomal organelles complex 3 subunit 2 2 2
MIRT446861 SAMD9L sterile alpha motif domain containing 9 like 2 2
MIRT450346 LHFPL3 LHFPL tetraspan subfamily member 3 2 2
MIRT454306 ZNF134 zinc finger protein 134 2 2
MIRT481951 ANKRD11 ankyrin repeat domain 11 2 2
MIRT484816 ZNFX1 zinc finger NFX1-type containing 1 2 2
MIRT497596 EBAG9 estrogen receptor binding site associated, antigen, 9 2 2
MIRT498620 MTRNR2L10 MT-RNR2-like 10 2 12
MIRT501852 MTRNR2L8 MT-RNR2-like 8 2 15
MIRT502628 DDX3X DEAD-box helicase 3, X-linked 2 8
MIRT503298 GTF2A1 general transcription factor IIA subunit 1 2 6
MIRT506789 KLHL15 kelch like family member 15 2 6
MIRT515069 PLA2G2C phospholipase A2 group IIC 2 2
MIRT521334 RPRD2 regulation of nuclear pre-mRNA domain containing 2 2 2
MIRT523692 FHL2 four and a half LIM domains 2 2 4
MIRT524416 CNOT6L CCR4-NOT transcription complex subunit 6 like 2 4
MIRT525526 FSIP2 fibrous sheath interacting protein 2 2 2
MIRT525771 SOD2 superoxide dismutase 2 2 2
MIRT526262 CCDC169 coiled-coil domain containing 169 2 2
MIRT526545 MDM2 MDM2 proto-oncogene 2 2
MIRT527522 CD59 CD59 molecule (CD59 blood group) 2 2
MIRT530875 TRUB1 TruB pseudouridine synthase family member 1 2 4
MIRT532991 ZKSCAN8 zinc finger with KRAB and SCAN domains 8 2 2
MIRT535581 NUP35 nucleoporin 35 2 2
MIRT536922 HEPHL1 hephaestin like 1 2 2
MIRT540004 NT5DC3 5'-nucleotidase domain containing 3 2 2
MIRT540779 SCO1 SCO1, cytochrome c oxidase assembly protein 2 2
MIRT541006 ZNF136 zinc finger protein 136 2 2
MIRT542072 ASS1 argininosuccinate synthase 1 2 6
MIRT544103 IPMK inositol polyphosphate multikinase 2 2
MIRT547669 KPNA1 karyopherin subunit alpha 1 2 4
MIRT549914 MRPS30 mitochondrial ribosomal protein S30 2 2
MIRT551741 HIST1H2BD histone cluster 1 H2B family member d 2 2
MIRT556374 LUZP1 leucine zipper protein 1 2 2
MIRT557320 HIC2 HIC ZBTB transcriptional repressor 2 2 2
MIRT561442 TOB2 transducer of ERBB2, 2 2 2
MIRT563938 TAF7 TATA-box binding protein associated factor 7 2 2
MIRT622315 SEH1L SEH1 like nucleoporin 2 2
MIRT622334 SEC63 SEC63 homolog, protein translocation regulator 2 2
MIRT627928 KANSL1 KAT8 regulatory NSL complex subunit 1 2 2
MIRT636122 YPEL1 yippee like 1 2 2
MIRT644871 C2orf48 chromosome 2 open reading frame 48 2 2
MIRT645105 LANCL3 LanC like 3 2 2
MIRT650430 BBS9 Bardet-Biedl syndrome 9 2 2
MIRT653330 SMIM18 small integral membrane protein 18 2 2
MIRT658101 FOXN3 forkhead box N3 2 2
MIRT690032 CCDC90B coiled-coil domain containing 90B 2 2
MIRT697283 ZNF800 zinc finger protein 800 2 2
MIRT700266 RAP2B RAP2B, member of RAS oncogene family 2 2
MIRT700310 RABGEF1 RAB guanine nucleotide exchange factor 1 2 2
MIRT705431 ATP11A ATPase phospholipid transporting 11A 2 2
MIRT706193 MED24 mediator complex subunit 24 2 2
MIRT707928 PPP1R3D protein phosphatase 1 regulatory subunit 3D 2 2
MIRT716123 RPL24 ribosomal protein L24 2 2
MIRT720940 PPP1R3E protein phosphatase 1 regulatory subunit 3E 2 2

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