pre-miRNA Information
pre-miRNA hsa-mir-4697   
Genomic Coordinates chr11: 133898504 - 133898581
Description Homo sapiens miR-4697 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4697-5p
Sequence 10| AGGGGGCGCAGUCACUGACGUG |31
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs549326907 2 dbSNP
rs897782230 7 dbSNP
rs748950280 8 dbSNP
rs978998780 15 dbSNP
rs968922289 19 dbSNP
rs3802919 20 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol NPBWR1
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HeLa
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in Chi_124B_2A8_130_50. RNA binding protein: AGO. Condition:HeLa cell miR-124 + B ...

- Chi SW; Zang JB; Mele A; Darnell RB, 2009, Nature.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' gugcagucacugaCGCGGGGGa 5'
                       ||| ::|| 
Target 5' -------gggcgcGCGGUUCCg 3'
1 - 15
Article - Chi SW; Zang JB; Mele A; Darnell RB
- Nature, 2009
MicroRNAs (miRNAs) have critical roles in the regulation of gene expression; however, as miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA, predicting target mRNAs is a major challenge. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein-RNA interaction sites. Here we use HITS-CLIP to covalently crosslink native argonaute (Ago, also called Eif2c) protein-RNA complexes in mouse brain. This produced two simultaneous data sets-Ago-miRNA and Ago-mRNA binding sites-that were combined with bioinformatic analysis to identify interaction sites between miRNA and target mRNA. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Ago HITS-CLIP provides a general platform for exploring the specificity and range of miRNA action in vivo, and identifies precise sequences for targeting clinically relevant miRNA-mRNA interactions.
LinkOut: [PMID: 19536157]
CLIP-seq Support 1 for dataset Chi_124B_2A8_130_50
Method / RBP HITS-CLIP / AGO
Cell line / Condition HeLa / HeLa cell miR-124 + B
Location of target site ENST00000331251.3 | 3UTR | GGGCGCGCGGUUCCGGCGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 19536157 / Chi_HITSCLIP
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
67 hsa-miR-4697-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT441332 C19orf26 CACN beta subunit associated regulatory protein 2 4
MIRT451390 FARSA phenylalanyl-tRNA synthetase alpha subunit 2 2
MIRT452310 EIF5AL1 eukaryotic translation initiation factor 5A-like 1 2 2
MIRT455043 MEN1 menin 1 2 2
MIRT455256 DDX39B DExD-box helicase 39B 2 10
MIRT461279 COX10 COX10, heme A:farnesyltransferase cytochrome c oxidase assembly factor 2 2
MIRT464945 TXLNA taxilin alpha 2 4
MIRT468043 SIK1 salt inducible kinase 1 2 2
MIRT472523 NACC1 nucleus accumbens associated 1 2 2
MIRT472929 MSN moesin 2 2
MIRT473247 MIDN midnolin 2 2
MIRT475828 HDGF heparin binding growth factor 2 2
MIRT478744 CS citrate synthase 2 2
MIRT480081 CALR calreticulin 2 2
MIRT483482 STMN3 stathmin 3 2 4
MIRT483643 QSOX2 quiescin sulfhydryl oxidase 2 2 4
MIRT483721 THSD4 thrombospondin type 1 domain containing 4 2 2
MIRT484539 BARHL1 BarH like homeobox 1 2 6
MIRT486041 WSCD1 WSC domain containing 1 2 4
MIRT486142 SIX5 SIX homeobox 5 2 6
MIRT486498 MYH11 myosin heavy chain 11 2 2
MIRT486977 STEAP3 STEAP3 metalloreductase 2 4
MIRT487279 AGPAT6 glycerol-3-phosphate acyltransferase 4 2 4
MIRT487742 MIB2 mindbomb E3 ubiquitin protein ligase 2 2 2
MIRT487987 RXRB retinoid X receptor beta 2 2
MIRT488338 PAX2 paired box 2 2 2
MIRT488668 WWP2 WW domain containing E3 ubiquitin protein ligase 2 2 4
MIRT488752 FXYD1 FXYD domain containing ion transport regulator 1 2 2
MIRT488812 TBC1D28 TBC1 domain family member 28 2 2
MIRT489380 RAB11B RAB11B, member RAS oncogene family 2 2
MIRT489744 TACC3 transforming acidic coiled-coil containing protein 3 2 2
MIRT489960 GNB2 G protein subunit beta 2 2 2
MIRT490418 VPS51 VPS51, GARP complex subunit 2 4
MIRT490639 FEM1A fem-1 homolog A 2 2
MIRT491086 MSI1 musashi RNA binding protein 1 2 4
MIRT491298 VGF VGF nerve growth factor inducible 2 4
MIRT491366 SLC12A5 solute carrier family 12 member 5 2 2
MIRT492464 RASD1 ras related dexamethasone induced 1 2 4
MIRT492872 NFIX nuclear factor I X 2 2
MIRT492951 NEUROD2 neuronal differentiation 2 2 2
MIRT493704 H2AFX H2A histone family member X 2 2
MIRT493977 EIF1 eukaryotic translation initiation factor 1 2 4
MIRT500360 ZNF385A zinc finger protein 385A 2 2
MIRT501154 SLC10A7 solute carrier family 10 member 7 2 6
MIRT509625 RRP7A ribosomal RNA processing 7 homolog A 2 4
MIRT512232 ATG2A autophagy related 2A 2 8
MIRT529822 ARGFX arginine-fifty homeobox 2 4
MIRT531179 SIGLEC12 sialic acid binding Ig like lectin 12 (gene/pseudogene) 2 2
MIRT538971 BCL7A BCL tumor suppressor 7A 2 2
MIRT548357 ENTPD5 ectonucleoside triphosphate diphosphohydrolase 5 2 4
MIRT553634 TJAP1 tight junction associated protein 1 2 2
MIRT558050 EVI5L ecotropic viral integration site 5 like 2 2
MIRT562548 CCDC71L coiled-coil domain containing 71 like 2 4
MIRT568941 RUNX3 runt related transcription factor 3 2 2
MIRT569114 ONECUT3 one cut homeobox 3 2 2
MIRT573591 CERS1 ceramide synthase 1 2 2
MIRT619317 ARHGAP18 Rho GTPase activating protein 18 2 2
MIRT621202 ARPC1B actin related protein 2/3 complex subunit 1B 2 2
MIRT628844 FAM151B family with sequence similarity 151 member B 2 2
MIRT670497 LYRM4 LYR motif containing 4 2 2
MIRT670545 SHISA2 shisa family member 2 2 2
MIRT671026 PCDHB2 protocadherin beta 2 2 2
MIRT688989 ATP6AP1 ATPase H+ transporting accessory protein 1 2 2
MIRT709275 MAPK8IP2 mitogen-activated protein kinase 8 interacting protein 2 2 2
MIRT715084 ELOF1 elongation factor 1 homolog 2 2
MIRT718348 NPBWR1 neuropeptides B and W receptor 1 2 2
MIRT737406 MMP7 matrix metallopeptidase 7 2 0
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-4697 Ceritinib 57379345 NSC776422 approved sensitive cell line (H3122)
hsa-miR-4697-5p Fulvestrant 17756771 NSC719276 approved resistant High Breast Cancer cell line (MCF-7)
hsa-miR-4697-5p Paclitaxel 36314 NSC125973 approved resistant High Breast Cancer cell line (Bads-200, Bats-72, BCap37)
hsa-miR-4697-5p Cisplatin 5460033 NSC119875 approved sensitive cell line (CAL-27) (total RNA)
hsa-miR-4697-5p Osimertinib 71496458 NSC779217 approved resistant cell line (H1975)
hsa-miR-4697-5p Cisplatin 5460033 NSC119875 approved resistant cell line (MGC-803)
hsa-miR-4697-5p Cisplatin 5460033 NSC119875 approved sensitive cell line (A2780)
hsa-miR-4697-5p Neoadjuvant chemotherapy resistant tissue (breast cancer)
hsa-miR-4697-5p Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (100 ng/ml)
hsa-miR-4697-5p Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (1500 ng/ml)

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