pre-miRNA Information
pre-miRNA hsa-mir-758   
Genomic Coordinates chr14: 101026020 - 101026107
Synonyms MIRN758, hsa-mir-758, MIR758
Description Homo sapiens miR-758 stem-loop
Comment None
RNA Secondary Structure
Associated Diseases

Mature miRNA Information
Mature miRNA hsa-miR-758-5p
Sequence 15| GAUGGUUGACCAGAGAGCACAC |36
Evidence Experimental
Experiments SOLiD
Editing Events in miRNAs
Modification Type Position on miR Chromosome DNA Strand Genomic Position (hg38) List of PMIDs Variant details
A-to-I 16 14 + 101026049 29233923 MiREDiBase
A-to-I 21 14 + 101026054 29233923 MiREDiBase
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs372417522 1 dbSNP
rs748092616 2 dbSNP
rs1270901332 4 dbSNP
rs1364206491 5 dbSNP
rs1483583954 8 dbSNP
rs770931807 10 dbSNP
rs776760808 11 dbSNP
rs1360350664 12 dbSNP
rs759742151 13 dbSNP
rs1002938972 15 dbSNP
rs770107042 17 dbSNP
rs369242818 20 dbSNP
rs1217090272 21 dbSNP
rs763341052 22 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
miRNAs in Extracellular Vesicles
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol TSPAN1   
Synonyms NET1, TM4C, TM4SF
Description tetraspanin 1
Transcript NM_005727   
Expression
Putative miRNA Targets on TSPAN1
3'UTR of TSPAN1
(miRNA target sites are highlighted)
>TSPAN1|NM_005727|3'UTR
   1 GTCCACTTCTGCCTCTGCCACTACTGCTGCCACATGGGAACTGTGAAGAGGCACCCTGGCAAGCAGCAGTGATTGGGGGA
  81 GGGGACAGGATCTAACAATGTCACTTGGGCCAGAATGGACCTGCCCTTTCTGCTCCAGACTTGGGGCTAGATAGGGACCA
 161 CTCCTTTTAGGCGATGCCTGACTTTCCTTCCATTGGTGGGTGGATGGGTGGGGGGCATTCCAGAGCCTCTAAGGTAGCCA
 241 GTTCTGTTGCCCATTCCCCCAGTCTATTAAACCCTTGATATGCCCCCTAGGCCTAGTGGTGATCCCAGTGCTCTACTGGG
 321 GGATGAGAGAAAGGCATTTTATAGCCTGGGCATAAGTGAAATCAGCAGAGCCTCTGGGTGGATGTGTAGAAGGCACTTCA
 401 AAATGCATAAACCTGTTACAATGTTGCCAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' cacaCGAGAGACCAGUUGGUag 5'
              || | |||| ||| ||  
Target 5' agagGCACCCTGG-CAAGCAgc 3'
47 - 67 109.00 -10.70
2
miRNA  3' cacacGAGAGACCAGUUGGUag 5'
               ||||  || :|:|||  
Target 5' agagcCTCTAAGG-TAGCCAgt 3'
222 - 242 104.00 -12.00
3
miRNA  3' cacACGAGAGACCAGUUGGUag 5'
             ||| |||||  | ||:|  
Target 5' ttcTGC-CTCTG--CCACTAct 3'
7 - 25 94.00 -8.30
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN27000268 39 COSMIC
COSN27000267 41 COSMIC
COSN23014370 52 COSMIC
COSN31612384 52 COSMIC
COSN30475678 85 COSMIC
COSN31517142 119 COSMIC
COSN30478900 126 COSMIC
COSN16128675 131 COSMIC
COSN31479707 139 COSMIC
COSN30104957 164 COSMIC
COSN32065131 369 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs1385327204 2 dbSNP
rs763432931 3 dbSNP
rs769019369 6 dbSNP
rs1162296389 8 dbSNP
rs1197120793 9 dbSNP
rs1489779213 10 dbSNP
rs774960760 13 dbSNP
rs760060391 15 dbSNP
rs199685801 18 dbSNP
rs1319795286 24 dbSNP
rs753427063 26 dbSNP
rs1221004399 31 dbSNP
rs759190086 32 dbSNP
rs1319943806 34 dbSNP
rs1317182350 39 dbSNP
rs1360289464 43 dbSNP
rs1236835755 48 dbSNP
rs929086591 50 dbSNP
rs201843939 51 dbSNP
rs1319090162 52 dbSNP
rs112015052 54 dbSNP
rs890342859 56 dbSNP
rs1444269612 58 dbSNP
rs941445938 59 dbSNP
rs973899735 60 dbSNP
rs1456976655 64 dbSNP
rs1347540895 65 dbSNP
rs865970135 68 dbSNP
rs1419987103 70 dbSNP
rs1379275133 77 dbSNP
rs1177238150 89 dbSNP
rs1802371 92 dbSNP
rs918408766 107 dbSNP
rs1490707132 115 dbSNP
rs1268684578 128 dbSNP
rs373512282 137 dbSNP
rs929867048 143 dbSNP
rs1288196575 145 dbSNP
rs1261811780 146 dbSNP
rs1353308175 153 dbSNP
rs1284141365 160 dbSNP
rs1236687226 165 dbSNP
rs556863534 165 dbSNP
rs1313672333 167 dbSNP
rs1301895681 169 dbSNP
rs1050989202 171 dbSNP
rs944628841 173 dbSNP
rs750243105 174 dbSNP
rs1400077978 178 dbSNP
rs1256810868 191 dbSNP
rs575522016 193 dbSNP
rs1156400033 194 dbSNP
rs1010896826 202 dbSNP
rs542554576 208 dbSNP
rs1378534084 210 dbSNP
rs892844981 210 dbSNP
rs1176006091 211 dbSNP
rs1011747802 212 dbSNP
rs902623329 216 dbSNP
rs1265933341 233 dbSNP
rs1000071848 245 dbSNP
rs1035181556 247 dbSNP
rs1802372 248 dbSNP
rs1261316233 250 dbSNP
rs1185197898 259 dbSNP
rs1202323151 261 dbSNP
rs1346895150 262 dbSNP
rs1802309 274 dbSNP
rs1256318080 275 dbSNP
rs902678577 277 dbSNP
rs1384377686 278 dbSNP
rs1321415258 280 dbSNP
rs11552232 283 dbSNP
rs1158182985 284 dbSNP
rs961183370 294 dbSNP
rs1361236137 301 dbSNP
rs879207176 310 dbSNP
rs1023926464 316 dbSNP
rs561305303 317 dbSNP
rs999671937 318 dbSNP
rs1433504853 321 dbSNP
rs567827047 328 dbSNP
rs960964630 330 dbSNP
rs528452773 334 dbSNP
rs1302673339 338 dbSNP
rs1464853380 347 dbSNP
rs1171937256 353 dbSNP
rs1426483164 353 dbSNP
rs1421517498 354 dbSNP
rs1189903021 376 dbSNP
rs1443259156 381 dbSNP
rs1255734532 383 dbSNP
rs1396937683 384 dbSNP
rs973069641 393 dbSNP
rs920319676 395 dbSNP
rs112046563 415 dbSNP
rs1160698768 418 dbSNP
rs1361219713 420 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HeLa
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in Chi_124A_2A8_130_50. RNA binding protein: AGO. Condition:HeLa cell miR-124 + A ...

- Chi SW; Zang JB; Mele A; Darnell RB, 2009, Nature.

Article - Chi SW; Zang JB; Mele A; Darnell RB
- Nature, 2009
MicroRNAs (miRNAs) have critical roles in the regulation of gene expression; however, as miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA, predicting target mRNAs is a major challenge. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein-RNA interaction sites. Here we use HITS-CLIP to covalently crosslink native argonaute (Ago, also called Eif2c) protein-RNA complexes in mouse brain. This produced two simultaneous data sets-Ago-miRNA and Ago-mRNA binding sites-that were combined with bioinformatic analysis to identify interaction sites between miRNA and target mRNA. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Ago HITS-CLIP provides a general platform for exploring the specificity and range of miRNA action in vivo, and identifies precise sequences for targeting clinically relevant miRNA-mRNA interactions.
LinkOut: [PMID: 19536157]
CLIP-seq Support 1 for dataset Chi_124A_2A8_130_50
Method / RBP HITS-CLIP / AGO
Cell line / Condition HeLa / HeLa cell miR-124 + A
Location of target site ENST00000355029.4 | 3UTR | CUUGGAAAACAAACC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 19536157 / Chi_HITSCLIP
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
62 hsa-miR-758-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT085323 MORC3 MORC family CW-type zinc finger 3 2 2
MIRT089441 STAMBP STAM binding protein 2 2
MIRT089456 TET3 tet methylcytosine dioxygenase 3 2 2
MIRT111856 CCND1 cyclin D1 2 2
MIRT184933 ZNF268 zinc finger protein 268 2 2
MIRT215288 CREBRF CREB3 regulatory factor 2 2
MIRT237300 LPP LIM domain containing preferred translocation partner in lipoma 2 2
MIRT238446 MYO10 myosin X 2 4
MIRT273827 RPL41 ribosomal protein L41 2 2
MIRT282703 HOOK1 hook microtubule tethering protein 1 2 2
MIRT347970 ZNF850 zinc finger protein 850 2 2
MIRT371076 KLF3 Kruppel like factor 3 2 2
MIRT464339 USP6NL USP6 N-terminal like 2 2
MIRT470034 PTP4A1 protein tyrosine phosphatase type IVA, member 1 2 2
MIRT477506 ELL2 elongation factor for RNA polymerase II 2 2 2
MIRT482886 CACNA2D3 calcium voltage-gated channel auxiliary subunit alpha2delta 3 2 2
MIRT492606 POLR3E RNA polymerase III subunit E 2 2
MIRT502294 GNG12 G protein subunit gamma 12 2 6
MIRT507600 DCTN4 dynactin subunit 4 2 4
MIRT510728 SON SON DNA binding protein 2 6
MIRT514065 KCNJ6 potassium voltage-gated channel subfamily J member 6 2 8
MIRT519718 ZNF512B zinc finger protein 512B 2 4
MIRT520890 STRN striatin 2 2
MIRT521760 PPIL1 peptidylprolyl isomerase like 1 2 6
MIRT526874 ERCC8 ERCC excision repair 8, CSA ubiquitin ligase complex subunit 2 2
MIRT530232 WSB2 WD repeat and SOCS box containing 2 2 2
MIRT532003 ACTR2 ARP2 actin related protein 2 homolog 2 2
MIRT533371 UBE2D4 ubiquitin conjugating enzyme E2 D4 (putative) 2 4
MIRT547106 PIGW phosphatidylinositol glycan anchor biosynthesis class W 2 2
MIRT548189 FOXA1 forkhead box A1 2 2
MIRT552935 VKORC1L1 vitamin K epoxide reductase complex subunit 1 like 1 2 2
MIRT560085 ZNF195 zinc finger protein 195 2 2
MIRT561726 PPP2CA protein phosphatase 2 catalytic subunit alpha 2 2
MIRT562713 ZNF415 zinc finger protein 415 2 2
MIRT562761 ZNF846 zinc finger protein 846 2 2
MIRT564159 ZNF117 zinc finger protein 117 2 2
MIRT565673 SETD5 SET domain containing 5 2 2
MIRT565718 SESN3 sestrin 3 2 2
MIRT566026 RFX1 regulatory factor X1 2 2
MIRT569048 ZNF655 zinc finger protein 655 2 2
MIRT570367 UBE2V1 ubiquitin conjugating enzyme E2 V1 2 2
MIRT570410 TMEM189-UBE2V1 TMEM189-UBE2V1 readthrough 2 2
MIRT570443 TMEM189 transmembrane protein 189 2 2
MIRT571738 RNF11 ring finger protein 11 2 2
MIRT575042 Tpgs2 tubulin polyglutamylase complex subunit 2 2 4
MIRT614330 ZDHHC22 zinc finger DHHC-type containing 22 2 2
MIRT617629 RAB3IP RAB3A interacting protein 2 2
MIRT621667 UBE4B ubiquitination factor E4B 2 2
MIRT639906 SRGAP2 SLIT-ROBO Rho GTPase activating protein 2 2 2
MIRT651436 XRCC5 X-ray repair cross complementing 5 2 2
MIRT683853 ZNF208 zinc finger protein 208 2 2
MIRT684841 TPGS2 tubulin polyglutamylase complex subunit 2 2 5
MIRT689347 ZNF83 zinc finger protein 83 2 2
MIRT692492 SPIN4 spindlin family member 4 2 2
MIRT695711 OLA1 Obg like ATPase 1 2 2
MIRT698219 TMEM248 transmembrane protein 248 2 2
MIRT711560 FAM20B FAM20B, glycosaminoglycan xylosylkinase 2 2
MIRT712867 TMEM67 transmembrane protein 67 2 2
MIRT722956 TSPAN1 tetraspanin 1 2 2
MIRT723622 SOBP sine oculis binding protein homolog 2 2
MIRT724176 ABCF2 ATP binding cassette subfamily F member 2 2 2
MIRT755363 LMBR1 limb development membrane protein 1 3 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-758 Hydroxycamptothecin (HCPT) NULL 97226 Microarray human Tenon's fibroblasts (HTFs) 24681041 2014 down-regulated
miR-758 Diethylstilbestrol approved 448537 Microarray mammosphere-derived epithelial cells (MDEC) 19549897 2009 up-regulated
miR-758 5-Fluorouracil approved 3385 Quantitative real-time PCR human squamous cell carcinoma cell line KYSE410 21743970 2011 up-regulated
miR-758 Reversine NULL 210332 Microarray C2C12 myoblast cells 24513286 2014 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-758 Ceritinib 57379345 NSC776422 approved resistant High Non-Small Cell Lung Cancer cell line (H3122, H2228)
hsa-mir-758 Fluorouracil 3385 NSC19893 approved resistant cell line (KYSE)
hsa-mir-758 Ceritinib 57379345 NSC776422 approved resistant cell line (H3122)
hsa-miR-758-5p Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-758-5p Osimertinib 71496458 NSC779217 approved sensitive cell line (HCC827)
hsa-miR-758-5p Gefitinib 123631 NSC715055 approved resistant cell line (HCC827)
hsa-miR-758-5p Paclitaxel 36314 NSC125973 approved sensitive cell line (BAS)
hsa-miR-758-5p Doxorubicin 31703 NSC123127 approved sensitive cell line (BAS)
hsa-miR-758-5p Osimertinib 71496458 NSC779217 approved resistant cell line (H1975)
hsa-miR-758-5p Gemcitabine 60750 NSC613327 approved resistant cell line (PANC-1) (1500 ng/ml)
hsa-miR-758-5p Ceritinib 57379345 NSC776422 approved resistant cell line (H3122)

Error report submission