pre-miRNA Information
pre-miRNA mmu-mir-302a   
Genomic Coordinates chr3: 127545496 - 127545564
Description Mus musculus miR-302a stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-302a-3p
Sequence 44| UAAGUGCUUCCAUGUUUUGGUGA |66
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Nr2f2   
Synonyms 2700033K02Rik, 9430015G03Rik, ARP-1, Aporp1, COUP-TF2, COUP-TFII, COUPTFB, EAR3, SVP40, Tcfcoup2
Description nuclear receptor subfamily 2, group F, member 2
Transcript NM_009697   
Other Transcripts NM_183261   
Expression
Putative miRNA Targets on Nr2f2
3'UTR of Nr2f2
(miRNA target sites are highlighted)
>Nr2f2|NM_009697|3'UTR
   1 ATAAATCAATCAAAATAAGGGGGAGTGAAACAGAGAAAGAAAAGGCAAAAGACTGGTTTTGTTTGCTTAATTTCCTTCTG
  81 TTAAGAAAGGATGTTACAAGTTTGCTAAAAAGAAGAGAGGGGAAGAATTTAATGGACTGTGAATTTCAAAAAGGAGAGAG
 161 AGAAAGAGAGAGACTGCCAAATGAACTTTTACAGAATGCATTAAAAAAAAAGAAAGAAAACAACTCCTGTGTTGGGCAGA
 241 ACAACCTGCTACTTATCATTTTTGTATAAAAAGGAAATTAGTCTTTTTTTCTTTTTGGTAAATTTTTGAAAAATATTGCT
 321 AAAAGTGCATTTAAGGAGATTGGGAGAAAATTAGCAGAATGGACAAAGTAAGTCATTTTTTTCCAAATTATTAATTGTCC
 401 TGTGTCTATGTACCTCTAGTTGTTCTTTTTTTTTTTTTTTAACTTTTCTGGTTCCAAACCAGTTTATTCTGTGGTTCAAT
 481 AATAAGTTTTGATATAATCTTGGCTTCTTAAAAACTGTGTATCATTAAAATATATGTTCTGCAAGAATTAAAACTGAGTC
 561 CATGAAAATAGCATAGGAAAACATAAAACTTTAAAAGGCAACTCAGAGATGGTGGAAATGCACTTACAAGTGGTGGCCAA
 641 ATTGTTTTTTTTTTTTTTTTTTTAAGGTAAAGTTGAGCACTCTAATTAGCAAGCTGGGGGAATCACATCAACACTTAGCT
 721 TCCCCACCCCCACCCCATACCATGACAAACCTAGCTTTTTAAAAAAAATATTTTAAGAAACAGAAGGAACTGTGGAATTT
 801 ATTGGCAGCCAAGGAATGTGTCCAAGACACAAGCTGAGGTTTTTGAATAAAAAGTGAACTTTTGTAATTTGAATTGGGTC
 881 CCCCCCCCTTAGTTCTTGAATTGTTATGAATCCTATATCTGTTTGTATATTTGCAAGCCCTTTGTATTATAATTGTTGAT
 961 ATTTCCCCTTTTTAAAAAATACCATTGAAATCAGCATGACAAAATAACACTGTTGGCACTTATAGGTAACGTGATTGATT
1041 CAGTATCTTAGAGTTTACAGTTTGTGTTTTTAAAAAACTGAAGGTTTTTTTTTTAAGTGCAACATTTCTGTATACTGTAA
1121 AAGTTATAATAACTGAACTGTTTGGTCGAGTCTTTGTGTGTTATATTCCAAGGAAATTGAAAGTATTCAGAAATTAAAAT
1201 ATTATTTGATATCTGAAATCTGCTTGGCTGTCCCCACTCACTGTCTTTCCACGGAGCTGAGCCCCTGTGAGTTCTCGCTG
1281 AGCCAGCGGGGGCCCCATTTGTTTACTCCCTCAATCAGTTTGTTCAAAGGTAGACTAGTGTATTTGCCTGTTTAATTTGG
1361 GTGTGGTGTGGGGGGGGAGCTGAAGTTAATGGTTTAGCTATGGTTTAGGAAGTGCCACACTGATATAGTAAGCCACCCCC
1441 ATTCACCTAATCCTACTTTTAATTAAAAATGGATTTTCCAGGAAAAAAATAAGGCCCTTATATTTGTCACACTTAAGTGC
1521 CTGCTTAGGGAAGGTATTGTGAAAAGTATTAGAAATTTTGAGATCAGTATCTGTTTTATGATCAGAAAAAAAATGCTCTT
1601 TTGTACATTTGTGACAGTTATGCAGAGGACTGTCCAAGCAAGCTAATCACAGAACTGTAAATAGAGGGCAGTTGTTTGCA
1681 ATGAGTTTTTCCTTAAGTAAGTGTAATTTTTCTTTTTCTTTTTTTCTTTTTTTTTTAAAAATATCCTGAGGTTCTCATTT
1761 AGCGTGGCTGTTGAGAGGATTTTGAATACAGTGATGTAGCTGCTAGCGACGAAGGGTCTGTTTTTCTTGTATATACATGA
1841 TAACTTGCAGTTGCCCTGCCTTTCCCCTCCCCCTCCCTCTTCAGTCTGTTGAGAGCATGGCCACAGGTCAAGGGAATCTT
1921 TACCATTGGAGTTATGTACATAAAAAAAAAAAACCATGAACATTTGGACATTTCAGATTATATAGAAACAATCTGTACTG
2001 CTCTGGGATCCTTTGGTCTTAGAAACCATTTTTGGGGGGGTGGAGAGAGAGAGAGGGAGAGGAGAGAGAGAGAGAGAGAG
2081 AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAATAAAGAAAACTTTACAGGGTGTGCTGATTTGGGAAG
2161 TCAACTATTTGGTTCTGTCCCTTATTCTCTTTTCCTGGTTTGGGAAGAGCTCACTGCTGGGAACTTGCAATTTGTTCTTA
2241 TTTAGACTTTCCAAGCTGCCCTCCCTGACAATACTTTTACCATGTTGTGGTTTAATCTTAAAACGGGGGAGGGGGCTGGT
2321 GACAGAGGTGAAAGAAAGGAGATCAGTTTGCCAAGTGCATTCAACTTTGATGCTCAGTTCTGGTTCATACATGCAGACCT
2401 GAAAACTCTGCCTGATTTAGGCAGAGATCTTTATCTGACCCTCAGCTTCCCTCTGTAGATATATAGATATATAAATATAA
2481 ATATGAATATAAGTATGTTTTACAGCACAGCATCTGACCTGTAGATGGAGGTTTTGTTGGTTGTTTATTTTCCCCTCTTG
2561 CAAGTGCTACCCATGTGAGTGTGTGAAGTTTCTCTACTAAGTAAAACACAGGCCCTTTTCCTTGTTTGCTTTGTGTTAGC
2641 TTATTGTAAACAGCCATTTGTTGTAAATTATTATTGGCATTAAATTATAATTTATGATTTTCAAAGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' agUGGUU--UUGU-ACCUUCGUGAAu 5'
            || ||  |||| ||  :|||||| 
Target 5' tgACAAAATAACACTGTTGGCACTTa 3'
997 - 1022 147.00 -13.50
2
miRNA  3' agUGGUUUUGUACC----UU-CGUGAAu 5'
            ||: |:| ||||    || |||||| 
Target 5' caACTCAGAGATGGTGGAAATGCACTTa 3'
599 - 626 135.00 -16.60
3
miRNA  3' aguGGUUUUGUACC--UUCGUGAau 5'
             ::||:::|  |  :||||||  
Target 5' tttTTAAGGTAAAGTTGAGCACTct 3'
659 - 683 130.00 -7.70
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions MC3T3-E1
Location of target site 3'UTR
Tools used in this research TargetScan
Original Description (Extracted from the article) ... "Transfection of miR-302a or BMP2 treatment in MC3T3-E1 cells increased cellular level of miR-302a (Fig. 5A and Supplementary Fig. 3A). COUP-TFII mRNA and protein levels were significantly decreased when cellular level of miR-302a was increased (Figs. 5B and C). To determine the specificity of miR-302a on COUP-TFII expression ...

- Kang IH; Jeong BC; Hur SW; Choi H; Choi SH; et al., 2015, Journal of cellular physiology.

Article - Kang IH; Jeong BC; Hur SW; Choi H; Choi SH; et al.
- Journal of cellular physiology, 2015
Chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) is a potent transcription factor that represses osteoblast differentiation and bone formation. Previously, we observed that stimuli for osteoblast differentiation, such as bone morphogenetic protein 2 (BMP2), inhibits COUP-TFII expression. This study was undertaken to identify BMP2-regulated and COUP-TFII-targeting microRNAs (miRNAs), and to explore their regulatory roles in osteoblast differentiation. Based on in silico analysis, 12 miRNAs were selected and their expression in BMP2-treated MC3T3-E1 cells was examined. BMP2 induced miR-302a expression in dose- and time-dependent manners with the decrease in COUP-TFII expression. Runx2, a BMP2-downstream transcription factor, specifically regulated miR-302a expression and its promoter activity. A computer-based prediction algorithm led to the identification of two miR-302a binding sites on the 3'-untranslational region of COUP-TFII mRNA (S1: 620-626 bp, S2: 1,016-1,022 bp), and a luciferase assay showed that miR-302a directly targeted S1 and S2. Transfection of miR-302a precursor significantly enhanced expression of osteogenic marker genes with decreasing COUP-TFII mRNA and protein level, alkaline phosphatase activity and matrix mineralization. On the other hand, inhibition of miR-302a significantly attenuated BMP2-induced osteoblast specific gene expression, alkaline phosphatase activity, and matrix mineralization with increasing COUP-TFII mRNA and protein level. These results indicate that miR-302a is induced by osteogenic stimuli and promotes osteoblast differentiation by targeting COUP-TFII. MiR-302a could be a positive regulator for osteoblast differentiation.
LinkOut: [PMID: 25215426]
73 mmu-miR-302a-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT054363 Lats2 large tumor suppressor 2 4 1
MIRT054577 Ccnd1 cyclin D1 4 1
MIRT578213 Myl6b myosin, light polypeptide 6B 1 1
MIRT579773 Zfp568 zinc finger protein 568 1 1
MIRT579903 Zbtb41 zinc finger and BTB domain containing 41 1 1
MIRT582206 Nfatc3 nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 1 1
MIRT589603 Luc7l2 LUC7-like 2 (S. cerevisiae) 1 4
MIRT592531 Mup7 major urinary protein 7 1 1
MIRT592581 Mup13 major urinary protein 13 1 1
MIRT595334 Galnt4 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 1 1
MIRT596464 Fam168b family with sequence similarity 168, member B 1 1
MIRT596522 Zfp882 zinc finger protein 882 1 1
MIRT596544 Zfp866 zinc finger protein 866 1 1
MIRT596766 Usp45 ubiquitin specific petidase 45 1 1
MIRT596795 Ubxn2a UBX domain protein 2A 1 1
MIRT596925 Tprkb Tp53rk binding protein 1 1
MIRT597148 Stxbp4 syntaxin binding protein 4 1 1
MIRT597693 Ppp2r3d protein phosphatase 2 (formerly 2A), regulatory subunit B'', delta 1 1
MIRT598003 Npy1r neuropeptide Y receptor Y1 1 1
MIRT598028 Nol10 nucleolar protein 10 1 1
MIRT598114 Ncl nucleolin 1 1
MIRT598150 Mtif2 mitochondrial translational initiation factor 2 1 1
MIRT598303 Mc1r melanocortin 1 receptor 1 1
MIRT598345 Mau2 MAU2 sister chromatid cohesion factor 1 1
MIRT598390 Magt1 magnesium transporter 1 1 1
MIRT598435 Lpl lipoprotein lipase 1 1
MIRT598657 Hpgds hematopoietic prostaglandin D synthase 1 1
MIRT598871 Glyctk glycerate kinase 1 1
MIRT598885 Glrx2 glutaredoxin 2 (thioltransferase) 1 1
MIRT599161 Diap2 diaphanous related formin 2 1 1
MIRT599255 Tmem245 transmembrane protein 245 1 1
MIRT599414 Chek2 checkpoint kinase 2 1 1
MIRT599539 Car5b carbonic anhydrase 5b, mitochondrial 1 1
MIRT599747 Anapc16 anaphase promoting complex subunit 16 1 1
MIRT599850 Adarb1 adenosine deaminase, RNA-specific, B1 1 1
MIRT599896 Acat1 acetyl-Coenzyme A acetyltransferase 1 1 1
MIRT599919 A830018L16Rik RIKEN cDNA A830018L16 gene 1 1
MIRT600228 Trim56 tripartite motif-containing 56 1 1
MIRT600269 Tns4 tensin 4 1 1
MIRT600435 Samd8 sterile alpha motif domain containing 8 1 1
MIRT600863 Gad2 glutamic acid decarboxylase 2 1 1
MIRT600876 Gabpb2 GA repeat binding protein, beta 2 1 1
MIRT600951 Elf1 E74-like factor 1 1 1
MIRT601038 Cntn2 contactin 2 1 1
MIRT601252 Abl2 v-abl Abelson murine leukemia viral oncogene 2 (arg, Abelson-related gene) 1 1
MIRT601369 Ybey ybeY metallopeptidase 1 1
MIRT601493 Tmlhe trimethyllysine hydroxylase, epsilon 1 1
MIRT601936 Ogdh oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 1 1
MIRT601974 Nav1 neuron navigator 1 1 1
MIRT602014 Mpp7 membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) 1 1
MIRT602186 Hyal1 hyaluronoglucosaminidase 1 1 1
MIRT602216 Hecw1 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 1 1
MIRT602228 H2-T3 histocompatibility 2, T region locus 3 1 1
MIRT602245 Grm1 glutamate receptor, metabotropic 1 1 1
MIRT602367 Fbxl3 F-box and leucine-rich repeat protein 3 1 1
MIRT602425 Ercc4 excision repair cross-complementing rodent repair deficiency, complementation group 4 1 1
MIRT602490 Dkc1 dyskeratosis congenita 1, dyskerin 1 1
MIRT602596 Chrnb4 cholinergic receptor, nicotinic, beta polypeptide 4 1 1
MIRT602684 Camk4 calcium/calmodulin-dependent protein kinase IV 1 1
MIRT602761 Asxl2 additional sex combs like 2 (Drosophila) 1 1
MIRT603033 Chic1 cysteine-rich hydrophobic domain 1 1 1
MIRT603526 Pwwp2a PWWP domain containing 2A 1 1
MIRT603813 Kif5b kinesin family member 5B 1 1
MIRT603883 Il10rb interleukin 10 receptor, beta 1 1
MIRT604185 Cxcl11 chemokine (C-X-C motif) ligand 11 1 1
MIRT605161 C030039L03Rik zinc finger protein 607B 1 1
MIRT605480 Slc6a2 solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 1 1
MIRT605613 Mpv17 MpV17 mitochondrial inner membrane protein 1 1
MIRT605789 Ddx11 DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 1 1
MIRT605806 Emc8 ER membrane protein complex subunit 8 1 1
MIRT605843 Cd300a CD300A molecule 1 1
MIRT731069 Nr2f2 nuclear receptor subfamily 2, group F, member 2 3 1
MIRT734874 FOXO1 forkhead box O1 3 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-302a Catechin approved 9064 Microarray apoE−/− mice 22253797 2012 up-regulated
miR-302a Glucose NULL 5793 Microarray endothelial cells 24394957 2014 up-regulated

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