pre-miRNA Information | |
---|---|
pre-miRNA | mmu-mir-302a |
Genomic Coordinates | chr3: 127545496 - 127545564 |
Description | Mus musculus miR-302a stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Mature miRNA Information | |
---|---|
Mature miRNA | mmu-miR-302a-3p |
Sequence | 44| UAAGUGCUUCCAUGUUUUGGUGA |66 |
Evidence | Experimental |
Experiments | Cloned |
Putative Targets |
Gene Information | |||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Gene Symbol | Nr2f2 | ||||||||||||||||||||
Synonyms | 2700033K02Rik, 9430015G03Rik, ARP-1, Aporp1, COUP-TF2, COUP-TFII, COUPTFB, EAR3, SVP40, Tcfcoup2 | ||||||||||||||||||||
Description | nuclear receptor subfamily 2, group F, member 2 | ||||||||||||||||||||
Transcript | NM_009697 | ||||||||||||||||||||
Other Transcripts | NM_183261 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on Nr2f2 | |||||||||||||||||||||
3'UTR of Nr2f2 (miRNA target sites are highlighted) |
>Nr2f2|NM_009697|3'UTR 1 ATAAATCAATCAAAATAAGGGGGAGTGAAACAGAGAAAGAAAAGGCAAAAGACTGGTTTTGTTTGCTTAATTTCCTTCTG 81 TTAAGAAAGGATGTTACAAGTTTGCTAAAAAGAAGAGAGGGGAAGAATTTAATGGACTGTGAATTTCAAAAAGGAGAGAG 161 AGAAAGAGAGAGACTGCCAAATGAACTTTTACAGAATGCATTAAAAAAAAAGAAAGAAAACAACTCCTGTGTTGGGCAGA 241 ACAACCTGCTACTTATCATTTTTGTATAAAAAGGAAATTAGTCTTTTTTTCTTTTTGGTAAATTTTTGAAAAATATTGCT 321 AAAAGTGCATTTAAGGAGATTGGGAGAAAATTAGCAGAATGGACAAAGTAAGTCATTTTTTTCCAAATTATTAATTGTCC 401 TGTGTCTATGTACCTCTAGTTGTTCTTTTTTTTTTTTTTTAACTTTTCTGGTTCCAAACCAGTTTATTCTGTGGTTCAAT 481 AATAAGTTTTGATATAATCTTGGCTTCTTAAAAACTGTGTATCATTAAAATATATGTTCTGCAAGAATTAAAACTGAGTC 561 CATGAAAATAGCATAGGAAAACATAAAACTTTAAAAGGCAACTCAGAGATGGTGGAAATGCACTTACAAGTGGTGGCCAA 641 ATTGTTTTTTTTTTTTTTTTTTTAAGGTAAAGTTGAGCACTCTAATTAGCAAGCTGGGGGAATCACATCAACACTTAGCT 721 TCCCCACCCCCACCCCATACCATGACAAACCTAGCTTTTTAAAAAAAATATTTTAAGAAACAGAAGGAACTGTGGAATTT 801 ATTGGCAGCCAAGGAATGTGTCCAAGACACAAGCTGAGGTTTTTGAATAAAAAGTGAACTTTTGTAATTTGAATTGGGTC 881 CCCCCCCCTTAGTTCTTGAATTGTTATGAATCCTATATCTGTTTGTATATTTGCAAGCCCTTTGTATTATAATTGTTGAT 961 ATTTCCCCTTTTTAAAAAATACCATTGAAATCAGCATGACAAAATAACACTGTTGGCACTTATAGGTAACGTGATTGATT 1041 CAGTATCTTAGAGTTTACAGTTTGTGTTTTTAAAAAACTGAAGGTTTTTTTTTTAAGTGCAACATTTCTGTATACTGTAA 1121 AAGTTATAATAACTGAACTGTTTGGTCGAGTCTTTGTGTGTTATATTCCAAGGAAATTGAAAGTATTCAGAAATTAAAAT 1201 ATTATTTGATATCTGAAATCTGCTTGGCTGTCCCCACTCACTGTCTTTCCACGGAGCTGAGCCCCTGTGAGTTCTCGCTG 1281 AGCCAGCGGGGGCCCCATTTGTTTACTCCCTCAATCAGTTTGTTCAAAGGTAGACTAGTGTATTTGCCTGTTTAATTTGG 1361 GTGTGGTGTGGGGGGGGAGCTGAAGTTAATGGTTTAGCTATGGTTTAGGAAGTGCCACACTGATATAGTAAGCCACCCCC 1441 ATTCACCTAATCCTACTTTTAATTAAAAATGGATTTTCCAGGAAAAAAATAAGGCCCTTATATTTGTCACACTTAAGTGC 1521 CTGCTTAGGGAAGGTATTGTGAAAAGTATTAGAAATTTTGAGATCAGTATCTGTTTTATGATCAGAAAAAAAATGCTCTT 1601 TTGTACATTTGTGACAGTTATGCAGAGGACTGTCCAAGCAAGCTAATCACAGAACTGTAAATAGAGGGCAGTTGTTTGCA 1681 ATGAGTTTTTCCTTAAGTAAGTGTAATTTTTCTTTTTCTTTTTTTCTTTTTTTTTTAAAAATATCCTGAGGTTCTCATTT 1761 AGCGTGGCTGTTGAGAGGATTTTGAATACAGTGATGTAGCTGCTAGCGACGAAGGGTCTGTTTTTCTTGTATATACATGA 1841 TAACTTGCAGTTGCCCTGCCTTTCCCCTCCCCCTCCCTCTTCAGTCTGTTGAGAGCATGGCCACAGGTCAAGGGAATCTT 1921 TACCATTGGAGTTATGTACATAAAAAAAAAAAACCATGAACATTTGGACATTTCAGATTATATAGAAACAATCTGTACTG 2001 CTCTGGGATCCTTTGGTCTTAGAAACCATTTTTGGGGGGGTGGAGAGAGAGAGAGGGAGAGGAGAGAGAGAGAGAGAGAG 2081 AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAATAAAGAAAACTTTACAGGGTGTGCTGATTTGGGAAG 2161 TCAACTATTTGGTTCTGTCCCTTATTCTCTTTTCCTGGTTTGGGAAGAGCTCACTGCTGGGAACTTGCAATTTGTTCTTA 2241 TTTAGACTTTCCAAGCTGCCCTCCCTGACAATACTTTTACCATGTTGTGGTTTAATCTTAAAACGGGGGAGGGGGCTGGT 2321 GACAGAGGTGAAAGAAAGGAGATCAGTTTGCCAAGTGCATTCAACTTTGATGCTCAGTTCTGGTTCATACATGCAGACCT 2401 GAAAACTCTGCCTGATTTAGGCAGAGATCTTTATCTGACCCTCAGCTTCCCTCTGTAGATATATAGATATATAAATATAA 2481 ATATGAATATAAGTATGTTTTACAGCACAGCATCTGACCTGTAGATGGAGGTTTTGTTGGTTGTTTATTTTCCCCTCTTG 2561 CAAGTGCTACCCATGTGAGTGTGTGAAGTTTCTCTACTAAGTAAAACACAGGCCCTTTTCCTTGTTTGCTTTGTGTTAGC 2641 TTATTGTAAACAGCCATTTGTTGTAAATTATTATTGGCATTAAATTATAATTTATGATTTTCAAAGC Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
|
||||||||||||||||||||
miRNA-target interactions (Predicted by miRanda) |
|
Experimental Support 1 for Functional miRNA-Target Interaction | |
---|---|
miRNA:Target | ---- |
Validation Method |
|
Conditions | MC3T3-E1 |
Location of target site | 3'UTR |
Tools used in this research | TargetScan |
Original Description (Extracted from the article) |
...
"Transfection of miR-302a or BMP2 treatment in MC3T3-E1 cells increased cellular level of miR-302a (Fig. 5A and Supplementary Fig. 3A). COUP-TFII mRNA and protein levels were significantly decreased when cellular level of miR-302a was increased (Figs. 5B and C). To determine the specificity of miR-302a on COUP-TFII expression
... - Kang IH; Jeong BC; Hur SW; Choi H; Choi SH; et al., 2015, Journal of cellular physiology. |
Article |
- Kang IH; Jeong BC; Hur SW; Choi H; Choi SH; et al. - Journal of cellular physiology, 2015
Chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) is a potent transcription factor that represses osteoblast differentiation and bone formation. Previously, we observed that stimuli for osteoblast differentiation, such as bone morphogenetic protein 2 (BMP2), inhibits COUP-TFII expression. This study was undertaken to identify BMP2-regulated and COUP-TFII-targeting microRNAs (miRNAs), and to explore their regulatory roles in osteoblast differentiation. Based on in silico analysis, 12 miRNAs were selected and their expression in BMP2-treated MC3T3-E1 cells was examined. BMP2 induced miR-302a expression in dose- and time-dependent manners with the decrease in COUP-TFII expression. Runx2, a BMP2-downstream transcription factor, specifically regulated miR-302a expression and its promoter activity. A computer-based prediction algorithm led to the identification of two miR-302a binding sites on the 3'-untranslational region of COUP-TFII mRNA (S1: 620-626 bp, S2: 1,016-1,022 bp), and a luciferase assay showed that miR-302a directly targeted S1 and S2. Transfection of miR-302a precursor significantly enhanced expression of osteogenic marker genes with decreasing COUP-TFII mRNA and protein level, alkaline phosphatase activity and matrix mineralization. On the other hand, inhibition of miR-302a significantly attenuated BMP2-induced osteoblast specific gene expression, alkaline phosphatase activity, and matrix mineralization with increasing COUP-TFII mRNA and protein level. These results indicate that miR-302a is induced by osteogenic stimuli and promotes osteoblast differentiation by targeting COUP-TFII. MiR-302a could be a positive regulator for osteoblast differentiation.
LinkOut: [PMID: 25215426]
|
73 mmu-miR-302a-3p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
![]() |
![]() |
|||||||
Strong evidence | Less strong evidence | |||||||||||
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
|||||
MIRT054363 | Lats2 | large tumor suppressor 2 | ![]() |
![]() |
![]() |
![]() |
4 | 1 | ||||
MIRT054577 | Ccnd1 | cyclin D1 | ![]() |
![]() |
![]() |
![]() |
4 | 1 | ||||
MIRT578213 | Myl6b | myosin, light polypeptide 6B | ![]() |
1 | 1 | |||||||
MIRT579773 | Zfp568 | zinc finger protein 568 | ![]() |
1 | 1 | |||||||
MIRT579903 | Zbtb41 | zinc finger and BTB domain containing 41 | ![]() |
1 | 1 | |||||||
MIRT582206 | Nfatc3 | nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 | ![]() |
1 | 1 | |||||||
MIRT589603 | Luc7l2 | LUC7-like 2 (S. cerevisiae) | ![]() |
1 | 4 | |||||||
MIRT592531 | Mup7 | major urinary protein 7 | ![]() |
1 | 1 | |||||||
MIRT592581 | Mup13 | major urinary protein 13 | ![]() |
1 | 1 | |||||||
MIRT595334 | Galnt4 | UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 | ![]() |
1 | 1 | |||||||
MIRT596464 | Fam168b | family with sequence similarity 168, member B | ![]() |
1 | 1 | |||||||
MIRT596522 | Zfp882 | zinc finger protein 882 | ![]() |
1 | 1 | |||||||
MIRT596544 | Zfp866 | zinc finger protein 866 | ![]() |
1 | 1 | |||||||
MIRT596766 | Usp45 | ubiquitin specific petidase 45 | ![]() |
1 | 1 | |||||||
MIRT596795 | Ubxn2a | UBX domain protein 2A | ![]() |
1 | 1 | |||||||
MIRT596925 | Tprkb | Tp53rk binding protein | ![]() |
1 | 1 | |||||||
MIRT597148 | Stxbp4 | syntaxin binding protein 4 | ![]() |
1 | 1 | |||||||
MIRT597693 | Ppp2r3d | protein phosphatase 2 (formerly 2A), regulatory subunit B'', delta | ![]() |
1 | 1 | |||||||
MIRT598003 | Npy1r | neuropeptide Y receptor Y1 | ![]() |
1 | 1 | |||||||
MIRT598028 | Nol10 | nucleolar protein 10 | ![]() |
1 | 1 | |||||||
MIRT598114 | Ncl | nucleolin | ![]() |
1 | 1 | |||||||
MIRT598150 | Mtif2 | mitochondrial translational initiation factor 2 | ![]() |
1 | 1 | |||||||
MIRT598303 | Mc1r | melanocortin 1 receptor | ![]() |
1 | 1 | |||||||
MIRT598345 | Mau2 | MAU2 sister chromatid cohesion factor | ![]() |
1 | 1 | |||||||
MIRT598390 | Magt1 | magnesium transporter 1 | ![]() |
1 | 1 | |||||||
MIRT598435 | Lpl | lipoprotein lipase | ![]() |
1 | 1 | |||||||
MIRT598657 | Hpgds | hematopoietic prostaglandin D synthase | ![]() |
1 | 1 | |||||||
MIRT598871 | Glyctk | glycerate kinase | ![]() |
1 | 1 | |||||||
MIRT598885 | Glrx2 | glutaredoxin 2 (thioltransferase) | ![]() |
1 | 1 | |||||||
MIRT599161 | Diap2 | diaphanous related formin 2 | ![]() |
1 | 1 | |||||||
MIRT599255 | Tmem245 | transmembrane protein 245 | ![]() |
1 | 1 | |||||||
MIRT599414 | Chek2 | checkpoint kinase 2 | ![]() |
1 | 1 | |||||||
MIRT599539 | Car5b | carbonic anhydrase 5b, mitochondrial | ![]() |
1 | 1 | |||||||
MIRT599747 | Anapc16 | anaphase promoting complex subunit 16 | ![]() |
1 | 1 | |||||||
MIRT599850 | Adarb1 | adenosine deaminase, RNA-specific, B1 | ![]() |
1 | 1 | |||||||
MIRT599896 | Acat1 | acetyl-Coenzyme A acetyltransferase 1 | ![]() |
1 | 1 | |||||||
MIRT599919 | A830018L16Rik | RIKEN cDNA A830018L16 gene | ![]() |
1 | 1 | |||||||
MIRT600228 | Trim56 | tripartite motif-containing 56 | ![]() |
1 | 1 | |||||||
MIRT600269 | Tns4 | tensin 4 | ![]() |
1 | 1 | |||||||
MIRT600435 | Samd8 | sterile alpha motif domain containing 8 | ![]() |
1 | 1 | |||||||
MIRT600863 | Gad2 | glutamic acid decarboxylase 2 | ![]() |
1 | 1 | |||||||
MIRT600876 | Gabpb2 | GA repeat binding protein, beta 2 | ![]() |
1 | 1 | |||||||
MIRT600951 | Elf1 | E74-like factor 1 | ![]() |
1 | 1 | |||||||
MIRT601038 | Cntn2 | contactin 2 | ![]() |
1 | 1 | |||||||
MIRT601252 | Abl2 | v-abl Abelson murine leukemia viral oncogene 2 (arg, Abelson-related gene) | ![]() |
1 | 1 | |||||||
MIRT601369 | Ybey | ybeY metallopeptidase | ![]() |
1 | 1 | |||||||
MIRT601493 | Tmlhe | trimethyllysine hydroxylase, epsilon | ![]() |
1 | 1 | |||||||
MIRT601936 | Ogdh | oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) | ![]() |
1 | 1 | |||||||
MIRT601974 | Nav1 | neuron navigator 1 | ![]() |
1 | 1 | |||||||
MIRT602014 | Mpp7 | membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) | ![]() |
1 | 1 | |||||||
MIRT602186 | Hyal1 | hyaluronoglucosaminidase 1 | ![]() |
1 | 1 | |||||||
MIRT602216 | Hecw1 | HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 | ![]() |
1 | 1 | |||||||
MIRT602228 | H2-T3 | histocompatibility 2, T region locus 3 | ![]() |
1 | 1 | |||||||
MIRT602245 | Grm1 | glutamate receptor, metabotropic 1 | ![]() |
1 | 1 | |||||||
MIRT602367 | Fbxl3 | F-box and leucine-rich repeat protein 3 | ![]() |
1 | 1 | |||||||
MIRT602425 | Ercc4 | excision repair cross-complementing rodent repair deficiency, complementation group 4 | ![]() |
1 | 1 | |||||||
MIRT602490 | Dkc1 | dyskeratosis congenita 1, dyskerin | ![]() |
1 | 1 | |||||||
MIRT602596 | Chrnb4 | cholinergic receptor, nicotinic, beta polypeptide 4 | ![]() |
1 | 1 | |||||||
MIRT602684 | Camk4 | calcium/calmodulin-dependent protein kinase IV | ![]() |
1 | 1 | |||||||
MIRT602761 | Asxl2 | additional sex combs like 2 (Drosophila) | ![]() |
1 | 1 | |||||||
MIRT603033 | Chic1 | cysteine-rich hydrophobic domain 1 | ![]() |
1 | 1 | |||||||
MIRT603526 | Pwwp2a | PWWP domain containing 2A | ![]() |
1 | 1 | |||||||
MIRT603813 | Kif5b | kinesin family member 5B | ![]() |
1 | 1 | |||||||
MIRT603883 | Il10rb | interleukin 10 receptor, beta | ![]() |
1 | 1 | |||||||
MIRT604185 | Cxcl11 | chemokine (C-X-C motif) ligand 11 | ![]() |
1 | 1 | |||||||
MIRT605161 | C030039L03Rik | zinc finger protein 607B | ![]() |
1 | 1 | |||||||
MIRT605480 | Slc6a2 | solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 | ![]() |
1 | 1 | |||||||
MIRT605613 | Mpv17 | MpV17 mitochondrial inner membrane protein | ![]() |
1 | 1 | |||||||
MIRT605789 | Ddx11 | DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 | ![]() |
1 | 1 | |||||||
MIRT605806 | Emc8 | ER membrane protein complex subunit 8 | ![]() |
1 | 1 | |||||||
MIRT605843 | Cd300a | CD300A molecule | ![]() |
1 | 1 | |||||||
MIRT731069 | Nr2f2 | nuclear receptor subfamily 2, group F, member 2 | ![]() |
![]() |
![]() |
3 | 1 | |||||
MIRT734874 | FOXO1 | forkhead box O1 | ![]() |
![]() |
![]() |
3 | 0 |