pre-miRNA Information | |
---|---|
pre-miRNA | mmu-mir-30a |
Genomic Coordinates | chr1: 23272269 - 23272339 |
Synonyms | Mirn30a, mmu-mir-30a, Mir30a |
Description | Mus musculus miR-30a stem-loop |
Comment | Landgraf et al. confirm that the 5' mature miRNA product is the predominant one . |
RNA Secondary Structure | ![]() |
Mature miRNA Information | |
---|---|
Mature miRNA | mmu-miR-30a-5p |
Sequence | 6| UGUAAACAUCCUCGACUGGAAG |27 |
Evidence | Experimental |
Experiments | Cloned |
Putative Targets |
Biomarker Information |
|
---|
Gene Information | |||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Gene Symbol | Il21r | ||||||||||||||||||||
Synonyms | NILR | ||||||||||||||||||||
Description | interleukin 21 receptor | ||||||||||||||||||||
Transcript | NM_021887 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on Il21r | |||||||||||||||||||||
3'UTR of Il21r (miRNA target sites are highlighted) |
>Il21r|NM_021887|3'UTR 1 CATATAATAACCAGCTATAGTGAGAAGAGGCCTCTGAGCCTGGCATTTACAGTGTGAACATGTAGGGGTGTGTGTGTGTG 81 TGTGTGTGTGTGTGTGTGTGTGTCTTGGGTTGTGTGTTAGCACATCCATGTTGGGATTTGGTCTGTTGCTATGTATTGTA 161 ATGCTAAATTCTCTACCCAAAGTTCTAGGCCTACGAGTGAATTCTCATGTTTACAAACTTGCTGTGTAAACCTTGTTCCT 241 TAATTTAATACCATTGGTTAAATAAAATTGGCTGCAACCAATTACTGGAGGGATTAGAGGTAGGGGGCTTTTGAGTTACC 321 TGTTTGGAGATGGAGAAGGAGAGAGGAGAGACCAAGAGGAGAAGGAGGAAGGAGAGGAGAGGAGAGGAGAGGAGAGGAGA 401 GGAGAGGAGAGGAGAGGAGAGGAGAGGAGAGGCTGCCGTGAGGGGAGAGGGACCATGAGCCTGTGGCCAGGAGAAACAGC 481 AAGTATCTGGGGTACACTGGTGAGGAGGTGGCCAGGCCAGCAGTTAGAAGAGTAGATTAGGGGTGACCTCCAGTATTTGT 561 CAAAGCCAATTAAAATAAC Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
|
||||||||||||||||||||
miRNA-target interactions (Predicted by miRanda) |
|
Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
---|---|---|---|---|---|---|---|
miRNA:Target | ---- | ||||||
Validation Method |
|
||||||
Conditions | 293T | ||||||
Disease | chronic autoimmune diseases//multiple sclerosis (MS) | ||||||
Location of target site | 3'UTR | ||||||
Tools used in this research | microRNA.org , TargetScan | ||||||
Original Description (Extracted from the article) |
...
our 茂卢聛ndings imply that miR-30a inhibits Th17 differentiation and the pathogenesis of MS by targeting IL-21R.
... - Qu X; Zhou J; Wang T; Han J; Ma L; Yu H; et al., 2016, Brain, behavior, and immunity. |
||||||
miRNA-target interactions (Provided by authors) |
|
||||||
Article |
- Qu X; Zhou J; Wang T; Han J; Ma L; Yu H; et al. - Brain, behavior, and immunity, 2016
T helper cells 17 (Th17) are recognized as key participants in the pathogenesis of chronic autoimmune diseases such as multiple sclerosis (MS). Regulation of Th17 differentiation is a valuable strategy for diagnosis and treatment of these complicated immune disorders. Here, by genome-wide expression profiling of microRNAs (miRs), we screened miR-30a, whose level was greatly decreased during Th17 differentiation and the process of demyelination disease, both in MS patients and experimental autoimmune encephalomyelitis (EAE) mice. Enforced constitutive expression of miR-30a in naive T cells inhibited their differentiation into Th17, and in vivo overexpression of miR-30a resulted in fewer Th17 and alleviative EAE. Moreover, target prediction analysis and dual luciferase report assay revealed that interleukin-21 receptor (IL-21R) was a direct target of miR-30a, a finding consistent with the results that miR-30a downregulated the expression of IL-21R, while overexpression of IL-21R alleviated the inhibitory effect of miR-30a on Th17 differentiation. Taken together, our findings imply that miR-30a inhibits Th17 differentiation and the pathogenesis of MS by targeting IL-21R.
LinkOut: [PMID: 27006279]
|
57 mmu-miR-30a-5p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
![]() |
![]() |
|||||||
Strong evidence | Less strong evidence | |||||||||||
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
|||||
MIRT003714 | Ak1 | adenylate kinase 1 | ![]() |
![]() |
![]() |
![]() |
4 | 1 | ||||
MIRT003715 | Tnrc6a | trinucleotide repeat containing 6a | ![]() |
![]() |
![]() |
![]() |
4 | 1 | ||||
MIRT003716 | Abcb1b | ATP-binding cassette, sub-family B (MDR/TAP), member 1B | ![]() |
![]() |
![]() |
3 | 1 | |||||
MIRT003717 | Inhba | inhibin beta-A | ![]() |
![]() |
![]() |
3 | 1 | |||||
MIRT003718 | Egfr | epidermal growth factor receptor | ![]() |
![]() |
![]() |
![]() |
4 | 2 | ||||
MIRT004770 | Bdnf | brain derived neurotrophic factor | ![]() |
![]() |
![]() |
![]() |
![]() |
5 | 2 | |||
MIRT007045 | Snai1 | snail family zinc finger 1 | ![]() |
![]() |
2 | 1 | ||||||
MIRT054524 | Ccne2 | cyclin E2 | ![]() |
![]() |
2 | 1 | ||||||
MIRT054525 | Celsr3 | cadherin, EGF LAG seven-pass G-type receptor 3 | ![]() |
![]() |
2 | 1 | ||||||
MIRT054526 | Mdm2 | transformed mouse 3T3 cell double minute 2 | ![]() |
![]() |
2 | 1 | ||||||
MIRT054527 | Mtdh | metadherin | ![]() |
![]() |
![]() |
3 | 2 | |||||
MIRT054528 | Smad1 | SMAD family member 1 | ![]() |
![]() |
2 | 1 | ||||||
MIRT054529 | Twf1 | twinfilin actin binding protein 1 | ![]() |
![]() |
2 | 1 | ||||||
MIRT054530 | Timp3 | tissue inhibitor of metalloproteinase 3 | ![]() |
![]() |
2 | 1 | ||||||
MIRT577999 | Pgr | progesterone receptor | ![]() |
1 | 1 | |||||||
MIRT578189 | Narg2 | interactor of little elongation complex ELL subunit 2 | ![]() |
1 | 1 | |||||||
MIRT578280 | Mesdc2 | mesoderm development LRP chaperone | ![]() |
1 | 1 | |||||||
MIRT578462 | Ints12 | integrator complex subunit 12 | ![]() |
1 | 1 | |||||||
MIRT578487 | Il18r1 | interleukin 18 receptor 1 | ![]() |
1 | 2 | |||||||
MIRT580036 | Wdr89 | WD repeat domain 89 | ![]() |
1 | 3 | |||||||
MIRT580042 | Wdr44 | WD repeat domain 44 | ![]() |
1 | 1 | |||||||
MIRT580299 | Tomm70a | translocase of outer mitochondrial membrane 70 homolog A (yeast) | ![]() |
1 | 1 | |||||||
MIRT581001 | Six4 | sine oculis-related homeobox 4 | ![]() |
1 | 1 | |||||||
MIRT581268 | Rrad | Ras-related associated with diabetes | ![]() |
1 | 1 | |||||||
MIRT582213 | Nfatc3 | nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 | ![]() |
1 | 1 | |||||||
MIRT582448 | Mgea5 | meningioma expressed antigen 5 (hyaluronidase) | ![]() |
1 | 1 | |||||||
MIRT583057 | Hoxb3 | homeobox B3 | ![]() |
1 | 1 | |||||||
MIRT583627 | Strip1 | striatin interacting protein 1 | ![]() |
1 | 1 | |||||||
MIRT583684 | Amer1 | APC membrane recruitment 1 | ![]() |
1 | 1 | |||||||
MIRT583926 | Dpy19l1 | dpy-19-like 1 (C. elegans) | ![]() |
1 | 1 | |||||||
MIRT584694 | Cep170b | centrosomal protein 170B | ![]() |
1 | 3 | |||||||
MIRT585022 | Gskip | GSK3B interacting protein | ![]() |
1 | 1 | |||||||
MIRT585237 | Zfp446 | zinc finger protein 446 | ![]() |
1 | 1 | |||||||
MIRT586099 | Reep4 | receptor accessory protein 4 | ![]() |
1 | 2 | |||||||
MIRT587711 | Cd300a | CD300A molecule | ![]() |
1 | 2 | |||||||
MIRT588338 | Zfp711 | zinc finger protein 711 | ![]() |
1 | 1 | |||||||
MIRT588632 | Tnrc6b | trinucleotide repeat containing 6b | ![]() |
1 | 3 | |||||||
MIRT589298 | Pigh | phosphatidylinositol glycan anchor biosynthesis, class H | ![]() |
1 | 1 | |||||||
MIRT590161 | Eno2 | enolase 2, gamma neuronal | ![]() |
1 | 1 | |||||||
MIRT592638 | Lpar3 | lysophosphatidic acid receptor 3 | ![]() |
1 | 2 | |||||||
MIRT593645 | Tbc1d5 | TBC1 domain family, member 5 | ![]() |
1 | 1 | |||||||
MIRT595042 | Camk4 | calcium/calmodulin-dependent protein kinase IV | ![]() |
1 | 1 | |||||||
MIRT595217 | Slc16a1 | solute carrier family 16 (monocarboxylic acid transporters), member 1 | ![]() |
1 | 1 | |||||||
MIRT595451 | Ptrhd1 | peptidyl-tRNA hydrolase domain containing 1 | ![]() |
1 | 1 | |||||||
MIRT595826 | Fam227a | family with sequence similarity 227, member A | ![]() |
1 | 1 | |||||||
MIRT596025 | Cdk13 | cyclin-dependent kinase 13 | ![]() |
1 | 1 | |||||||
MIRT596356 | Serpine1 | serine (or cysteine) peptidase inhibitor, clade E, member 1 | ![]() |
1 | 1 | |||||||
MIRT596419 | Ing1 | inhibitor of growth family, member 1 | ![]() |
1 | 1 | |||||||
MIRT596513 | Zfp9 | zinc finger protein 9 | ![]() |
1 | 1 | |||||||
MIRT599308 | Csf1 | colony stimulating factor 1 (macrophage) | ![]() |
1 | 1 | |||||||
MIRT603175 | Trp53rk | transformation related protein 53 regulating kinase B | ![]() |
1 | 1 | |||||||
MIRT731329 | IL21R | interleukin 21 receptor | ![]() |
1 | 1 | |||||||
MIRT733494 | Gfra1 | glial cell line derived neurotrophic factor family receptor alpha 1 | ![]() |
1 | 0 | |||||||
MIRT735519 | SOCS1 | suppressor of cytokine signaling 1 | ![]() |
![]() |
2 | 0 | ||||||
MIRT755305 | Runx2 | runt related transcription factor 2 | 2 | 1 | ||||||||
MIRT755548 | SOCS3 | suppressor of cytokine signaling 3 | 3 | 1 | ||||||||
MIRT755880 | NCAM1 | neural cell adhesion molecule 1 | 1 | 1 |
miRNA-Drug Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|