miRTarBase - #MIRT735453


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pre-miRNA Information
pre-miRNA mmu-mir-143   
Genomic Coordinates chr18: 61649196 - 61649258
Synonyms Mirn143, mmu-mir-143, Mir143
Description Mus musculus miR-143 stem-loop
Comment Expression of this miRNA in mouse was independently verified in heart and brain .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-143-3p
Sequence 40| UGAGAUGAAGCACUGUAGCUC |60
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
B7G3SK miR-143 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Lower Blood Real-time polymerase chain reaction
B7G3SK miR-143 Predictive Biomarker (PRD); Safety Biomarker (SAF) Transcriptomic Data . . . .
Gene Information
Gene Symbol Col5a2   
Synonyms 1110014L14Rik
Description collagen, type V, alpha 2
Transcript NM_007737   
Expression
Putative miRNA Targets on Col5a2
3'UTR of Col5a2
(miRNA target sites are highlighted)		 
>Col5a2|NM_007737|3'UTR
   1 CAGAAGCCCAGACATATCGACAATGAGCACTACCATCAATGACCACCACAAGAACTGTGACTGTTTAAAGTTCATCCTGA
  81 GACTCTTGAAGTAATGGCTGGTCCTGCATCAGCTTTGTACATACGATCTGAAGTGCCTGACCACCTTATCCTTCAGAATA
 161 TTTATTTTACTTACAATCCTCAAGTTTAATTGACTTAATTTTTTCAATATGGCCGTTTAGATTAAAGCAACCAATGACAA
 241 TGACCATCTTTACAAAAAGTAAACTGATCAAAAAATAAATATGTTTTCATCAATTCATTTCACTGTAATGAATACGTTGC
 321 TTAGTATTTATGAGAAAATTCTTCTTCCTAGCAGGTAGCTTACATGATGGGGTATGTAAAGCCTCAGCATGTGTTGATTT
 401 TGCTTGGCTGCAGTCTGAAAAGTAGAGAGTAATGCCCCCTGTGTCCTCTTATTGTCAGAACATCAGTGACAAATGAAGAC
 481 CCCCATGCATGTCTTAATATTATACTAACATTATAGAAAAAATTAAGGTGAATATAACACGTTTCCACTGTCTTAAATGA
 561 TATCTTTAAATAACATGTAACCATTGACTTTTAGGAGGAAAAAAATCCATAAAATTGCTTCAACTTTACAATTTATTTTG
 641 CAAAAACTGATACAGTTAGAGTTTTTTCCTCATTCAAATTTAAGGGTAACATTTTCTTTTTGTTGTAATCTTTCAATACA
 721 GATAGAAGTTATATTTGTGGTTTCCTATGTAAGAAGATATTGGATGAAATTAAAAAAAATTACCCACACAATTTTCTAAC
 801 ACACGTGCCCAGTAATGAATGTTAAGACTCTCCCACAGTCACAGTCTCATTTTCCTGAAGCATATCATAAGAAATGAACA
 881 AGAAGTCCAGCATTATAATGATCACTCAAATAAAATGTGAGGATTGGAATTAGAACATTCATAAGAAAATATGACCCAAC
 961 ATTTCTTAGCATGACCTACCTCATCTCTGCAAGCTGGGATAGATTTCCTGTTCTTGTTTATTTTTAGGTGTTGAAAGGTG
1041 CTATGCTTCTGTTGTTATTCCAAGACTGGAGATAGGCAGGGTTAAAAATATTATAACTTTTCTTTTAATGATGGTGCTAA
1121 AATTATTTCTATAAAATTCTTTAAAGATAAAGATTGTTTCATCAATGCTATTTGTGAACACAGAACTGTTAAACATCCCA
1201 TTTGAGAGATAGATCATTATTCCTGGGTCTTTTAACTGTTCCTTTTATGATGTATTTAGAACATTGTGTAATGCTTGCAA
1281 GTGTCTTAACCTAGGACACATAGGTCCCCTTGTGTCTCAAGATTTTTTCCTTAATTGCAGTTGTTTGCTCCATTGTTGTT
1361 TCTTACTTAAATAATCTGGGCCATCCCAAAGGACAAGGCCCACATAGAACTTTGATCATGTATCTCTGCAAAGCATCGAA
1441 TTAAATGCATGCTTCTGTCATGTCAGTCATCTTCAGTTTGTTGAATCCTTTGAACATATTAGGTCCTTTTAGTTTCCAGG
1521 AGTGAAAACTAAGATCTCCTGGTAGTTTTTGTTCACAATTTAGTAGACGATGCAACAAGTACCTACCATTTTGTCTGCAC
1601 TGGCTTAAAAATAAACCTATCTGTAACATTGCCTGCAAAGGCATGTTGACATCAATGAAGACAGTCTATGCGTTAGAAAA
1681 CTCAGGCTAGTGATTGGTAAAGAGTAAAGGAAATGAAAAATCCATTATTGAATAAACTAATAATAAAACATGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' cucgaugucACGAAGUAGAGu 5'
                   | | ||||||| 
Target 5' agcatgaccTACCTCATCTCt 3'
 968 - 988 144.00 -9.50
2 
miRNA  3' cuCGAUGUCACGA-----AGUAGAGu 5'
            ||| | ||  |     ||||||: 
Target 5' atGCTTCTGTCATGTCAGTCATCTTc 3'
 1449 - 1474 131.00 -9.70
3 
miRNA  3' cucGAUGUCACGAAGUAGAGu 5'
             :| ||    |||||:|| 
Target 5' tgtTTTCATCAATTCATTTCa 3'
 282 - 302 130.00 -8.50
24 mmu-miR-143-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004492 Prkce protein kinase C, epsilon 4 1
MIRT004532 Vcan versican 4 1
MIRT004772 Elk1 ELK1, member of ETS oncogene family 4 1
MIRT006031 Kras Kirsten rat sarcoma viral oncogene homolog 5 1
MIRT006032 Rreb1 ras responsive element binding protein 1 5 1
MIRT006121 Ptn pleiotrophin 3 1
MIRT054755 Igfbp5 insulin-like growth factor binding protein 5 2 1
MIRT586427 Ogfrl1 opioid growth factor receptor-like 1 1 1
MIRT589192 Ptpn3 protein tyrosine phosphatase, non-receptor type 3 1 1
MIRT591160 Myo5c myosin VC 1 1
MIRT593574 Zyg11b zyg-ll family member B, cell cycle regulator 1 1
MIRT594328 Nav1 neuron navigator 1 1 1
MIRT595606 Mapk8 mitogen-activated protein kinase 8 1 1
MIRT596433 Crebzf CREB/ATF bZIP transcription factor 1 1
MIRT596597 Zfp488 zinc finger protein 488 1 1
MIRT599868 Acnat1 acyl-coenzyme A amino acid N-acyltransferase 1 1 1
MIRT600661 March5 membrane-associated ring finger (C3HC4) 5 1 1
MIRT602320 Gas2l3 growth arrest-specific 2 like 3 1 1
MIRT604071 Egln2 egl-9 family hypoxia-inducible factor 2 1 1
MIRT731148 Dnmt3a DNA methyltransferase 3A 1 1
MIRT733322 Sox2 SRY (sex determining region Y)-box 2 2 0
MIRT734441 Map3k7 mitogen-activated protein kinase kinase kinase 7 2 0
MIRT735453 COL5A2 collagen type V alpha 2 chain 1 0
MIRT756168 Npy neuropeptide Y 2 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-143 Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse liver 19270793 2009 up-regulated
miR-143 Trichostatin A (TSA) NULL 444732 Quantitative real-time PCR myocardial differentiation of mouse ES cells 19521018 2009 down-regulated
miR-143 Sodium butyrate NULL 5222465 Quantitative real-time PCR Burkitt lymphoma cells 24577510 2014 up-regulated
miR-143 XMD8-92 NULL 46843772 Microarray pancreatic ductal adenocarcinoma cell 24880079 2014 up-regulated
miR-143 Medpor NULL NULL Microarray osteoblast-like cells line (MG-63) 18408260 2008 down-regulated
miR-143 17beta-estradiol (E2) approved 5757 Microarray MCF-7AKT breast cancer cells 19528081 2009 down-regulated
miR-143 Enoxacin approved 3229 Quantitative real-time PCR HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-143 Trichostatin A (TSA) NULL 444732 Microarray apoptosis-resistant breast cancer cells 21971930 2011 up-regulated
miR-143 Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-143 Estrogen NULL NULL Quantitative real-time PCR breast cancer cells. 22260523 2012 down-regulated
miR-143 1,2,6-Tri-O-galloyl-beta-D-glucopyranose NULL NULL Microarray HepG2 hepatocarcinoma cells. 22506400 2011 down-regulated
miR-143 Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 up-regulated
miR-143 Conjugated linoleic acid NULL NULL Quantitative real-time PCR retroperitoneal adipose tissue 20886002 2010 down-regulated
miR-143 Ursodeoxycholic acid (UDCA) approved 31401 Microarray rat Liver 20689055 2010 up-regulated
miR-143-3p Docosahexaenoic acid NULL 445580 Quantitative real-time PCR Caco-2 cells 24623846 2014 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-143-3p Doxorubicin 31703 NSC123127 approved sensitive Low Osteosarcoma tissue and cell line (SAOS-2, U-2-OS)

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